analysis of data example in research

Data Analysis Techniques in Research – Methods, Tools & Examples

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Data analysis techniques in research are essential because they allow researchers to derive meaningful insights from data sets to support their hypotheses or research objectives.

Data Analysis Techniques in Research : While various groups, institutions, and professionals may have diverse approaches to data analysis, a universal definition captures its essence. Data analysis involves refining, transforming, and interpreting raw data to derive actionable insights that guide informed decision-making for businesses.

A straightforward illustration of data analysis emerges when we make everyday decisions, basing our choices on past experiences or predictions of potential outcomes.

If you want to learn more about this topic and acquire valuable skills that will set you apart in today’s data-driven world, we highly recommend enrolling in the Data Analytics Course by Physics Wallah . And as a special offer for our readers, use the coupon code “READER” to get a discount on this course.

Table of Contents

What is Data Analysis?

Data analysis is the systematic process of inspecting, cleaning, transforming, and interpreting data with the objective of discovering valuable insights and drawing meaningful conclusions. This process involves several steps:

• Inspecting : Initial examination of data to understand its structure, quality, and completeness.
• Cleaning : Removing errors, inconsistencies, or irrelevant information to ensure accurate analysis.
• Transforming : Converting data into a format suitable for analysis, such as normalization or aggregation.
• Interpreting : Analyzing the transformed data to identify patterns, trends, and relationships.

Types of Data Analysis Techniques in Research

Data analysis techniques in research are categorized into qualitative and quantitative methods, each with its specific approaches and tools. These techniques are instrumental in extracting meaningful insights, patterns, and relationships from data to support informed decision-making, validate hypotheses, and derive actionable recommendations. Below is an in-depth exploration of the various types of data analysis techniques commonly employed in research:

1) Qualitative Analysis:

Definition: Qualitative analysis focuses on understanding non-numerical data, such as opinions, concepts, or experiences, to derive insights into human behavior, attitudes, and perceptions.

• Content Analysis: Examines textual data, such as interview transcripts, articles, or open-ended survey responses, to identify themes, patterns, or trends.
• Narrative Analysis: Analyzes personal stories or narratives to understand individuals’ experiences, emotions, or perspectives.
• Ethnographic Studies: Involves observing and analyzing cultural practices, behaviors, and norms within specific communities or settings.

2) Quantitative Analysis:

Quantitative analysis emphasizes numerical data and employs statistical methods to explore relationships, patterns, and trends. It encompasses several approaches:

Descriptive Analysis:

• Frequency Distribution: Represents the number of occurrences of distinct values within a dataset.
• Central Tendency: Measures such as mean, median, and mode provide insights into the central values of a dataset.
• Dispersion: Techniques like variance and standard deviation indicate the spread or variability of data.

Diagnostic Analysis:

• Regression Analysis: Assesses the relationship between dependent and independent variables, enabling prediction or understanding causality.
• ANOVA (Analysis of Variance): Examines differences between groups to identify significant variations or effects.

Predictive Analysis:

• Time Series Forecasting: Uses historical data points to predict future trends or outcomes.
• Machine Learning Algorithms: Techniques like decision trees, random forests, and neural networks predict outcomes based on patterns in data.

Prescriptive Analysis:

• Optimization Models: Utilizes linear programming, integer programming, or other optimization techniques to identify the best solutions or strategies.
• Simulation: Mimics real-world scenarios to evaluate various strategies or decisions and determine optimal outcomes.

Specific Techniques:

• Monte Carlo Simulation: Models probabilistic outcomes to assess risk and uncertainty.
• Factor Analysis: Reduces the dimensionality of data by identifying underlying factors or components.
• Cohort Analysis: Studies specific groups or cohorts over time to understand trends, behaviors, or patterns within these groups.
• Cluster Analysis: Classifies objects or individuals into homogeneous groups or clusters based on similarities or attributes.
• Sentiment Analysis: Uses natural language processing and machine learning techniques to determine sentiment, emotions, or opinions from textual data.

Also Read: AI and Predictive Analytics: Examples, Tools, Uses, Ai Vs Predictive Analytics

Data Analysis Techniques in Research Examples

To provide a clearer understanding of how data analysis techniques are applied in research, let’s consider a hypothetical research study focused on evaluating the impact of online learning platforms on students’ academic performance.

Research Objective:

Determine if students using online learning platforms achieve higher academic performance compared to those relying solely on traditional classroom instruction.

Data Collection:

• Quantitative Data: Academic scores (grades) of students using online platforms and those using traditional classroom methods.
• Qualitative Data: Feedback from students regarding their learning experiences, challenges faced, and preferences.

Data Analysis Techniques Applied:

1) Descriptive Analysis:

• Calculate the mean, median, and mode of academic scores for both groups.
• Create frequency distributions to represent the distribution of grades in each group.

2) Diagnostic Analysis:

• Conduct an Analysis of Variance (ANOVA) to determine if there’s a statistically significant difference in academic scores between the two groups.
• Perform Regression Analysis to assess the relationship between the time spent on online platforms and academic performance.

3) Predictive Analysis:

• Utilize Time Series Forecasting to predict future academic performance trends based on historical data.
• Implement Machine Learning algorithms to develop a predictive model that identifies factors contributing to academic success on online platforms.

4) Prescriptive Analysis:

• Apply Optimization Models to identify the optimal combination of online learning resources (e.g., video lectures, interactive quizzes) that maximize academic performance.
• Use Simulation Techniques to evaluate different scenarios, such as varying student engagement levels with online resources, to determine the most effective strategies for improving learning outcomes.

5) Specific Techniques:

• Conduct Factor Analysis on qualitative feedback to identify common themes or factors influencing students’ perceptions and experiences with online learning.
• Perform Cluster Analysis to segment students based on their engagement levels, preferences, or academic outcomes, enabling targeted interventions or personalized learning strategies.
• Apply Sentiment Analysis on textual feedback to categorize students’ sentiments as positive, negative, or neutral regarding online learning experiences.

By applying a combination of qualitative and quantitative data analysis techniques, this research example aims to provide comprehensive insights into the effectiveness of online learning platforms.

Also Read: Learning Path to Become a Data Analyst in 2024

Data Analysis Techniques in Quantitative Research

Quantitative research involves collecting numerical data to examine relationships, test hypotheses, and make predictions. Various data analysis techniques are employed to interpret and draw conclusions from quantitative data. Here are some key data analysis techniques commonly used in quantitative research:

1) Descriptive Statistics:

• Description: Descriptive statistics are used to summarize and describe the main aspects of a dataset, such as central tendency (mean, median, mode), variability (range, variance, standard deviation), and distribution (skewness, kurtosis).
• Applications: Summarizing data, identifying patterns, and providing initial insights into the dataset.

2) Inferential Statistics:

• Description: Inferential statistics involve making predictions or inferences about a population based on a sample of data. This technique includes hypothesis testing, confidence intervals, t-tests, chi-square tests, analysis of variance (ANOVA), regression analysis, and correlation analysis.
• Applications: Testing hypotheses, making predictions, and generalizing findings from a sample to a larger population.

3) Regression Analysis:

• Description: Regression analysis is a statistical technique used to model and examine the relationship between a dependent variable and one or more independent variables. Linear regression, multiple regression, logistic regression, and nonlinear regression are common types of regression analysis .
• Applications: Predicting outcomes, identifying relationships between variables, and understanding the impact of independent variables on the dependent variable.

4) Correlation Analysis:

• Description: Correlation analysis is used to measure and assess the strength and direction of the relationship between two or more variables. The Pearson correlation coefficient, Spearman rank correlation coefficient, and Kendall’s tau are commonly used measures of correlation.
• Applications: Identifying associations between variables and assessing the degree and nature of the relationship.

5) Factor Analysis:

• Description: Factor analysis is a multivariate statistical technique used to identify and analyze underlying relationships or factors among a set of observed variables. It helps in reducing the dimensionality of data and identifying latent variables or constructs.
• Applications: Identifying underlying factors or constructs, simplifying data structures, and understanding the underlying relationships among variables.

6) Time Series Analysis:

• Description: Time series analysis involves analyzing data collected or recorded over a specific period at regular intervals to identify patterns, trends, and seasonality. Techniques such as moving averages, exponential smoothing, autoregressive integrated moving average (ARIMA), and Fourier analysis are used.
• Applications: Forecasting future trends, analyzing seasonal patterns, and understanding time-dependent relationships in data.

7) ANOVA (Analysis of Variance):

• Description: Analysis of variance (ANOVA) is a statistical technique used to analyze and compare the means of two or more groups or treatments to determine if they are statistically different from each other. One-way ANOVA, two-way ANOVA, and MANOVA (Multivariate Analysis of Variance) are common types of ANOVA.
• Applications: Comparing group means, testing hypotheses, and determining the effects of categorical independent variables on a continuous dependent variable.

8) Chi-Square Tests:

• Description: Chi-square tests are non-parametric statistical tests used to assess the association between categorical variables in a contingency table. The Chi-square test of independence, goodness-of-fit test, and test of homogeneity are common chi-square tests.
• Applications: Testing relationships between categorical variables, assessing goodness-of-fit, and evaluating independence.

These quantitative data analysis techniques provide researchers with valuable tools and methods to analyze, interpret, and derive meaningful insights from numerical data. The selection of a specific technique often depends on the research objectives, the nature of the data, and the underlying assumptions of the statistical methods being used.

Also Read: Analysis vs. Analytics: How Are They Different?

Data Analysis Methods

Data analysis methods refer to the techniques and procedures used to analyze, interpret, and draw conclusions from data. These methods are essential for transforming raw data into meaningful insights, facilitating decision-making processes, and driving strategies across various fields. Here are some common data analysis methods:

• Description: Descriptive statistics summarize and organize data to provide a clear and concise overview of the dataset. Measures such as mean, median, mode, range, variance, and standard deviation are commonly used.
• Description: Inferential statistics involve making predictions or inferences about a population based on a sample of data. Techniques such as hypothesis testing, confidence intervals, and regression analysis are used.

3) Exploratory Data Analysis (EDA):

• Description: EDA techniques involve visually exploring and analyzing data to discover patterns, relationships, anomalies, and insights. Methods such as scatter plots, histograms, box plots, and correlation matrices are utilized.
• Applications: Identifying trends, patterns, outliers, and relationships within the dataset.

4) Predictive Analytics:

• Description: Predictive analytics use statistical algorithms and machine learning techniques to analyze historical data and make predictions about future events or outcomes. Techniques such as regression analysis, time series forecasting, and machine learning algorithms (e.g., decision trees, random forests, neural networks) are employed.
• Applications: Forecasting future trends, predicting outcomes, and identifying potential risks or opportunities.

5) Prescriptive Analytics:

• Description: Prescriptive analytics involve analyzing data to recommend actions or strategies that optimize specific objectives or outcomes. Optimization techniques, simulation models, and decision-making algorithms are utilized.
• Applications: Recommending optimal strategies, decision-making support, and resource allocation.

6) Qualitative Data Analysis:

• Description: Qualitative data analysis involves analyzing non-numerical data, such as text, images, videos, or audio, to identify themes, patterns, and insights. Methods such as content analysis, thematic analysis, and narrative analysis are used.
• Applications: Understanding human behavior, attitudes, perceptions, and experiences.

7) Big Data Analytics:

• Description: Big data analytics methods are designed to analyze large volumes of structured and unstructured data to extract valuable insights. Technologies such as Hadoop, Spark, and NoSQL databases are used to process and analyze big data.
• Applications: Analyzing large datasets, identifying trends, patterns, and insights from big data sources.

8) Text Analytics:

• Description: Text analytics methods involve analyzing textual data, such as customer reviews, social media posts, emails, and documents, to extract meaningful information and insights. Techniques such as sentiment analysis, text mining, and natural language processing (NLP) are used.
• Applications: Analyzing customer feedback, monitoring brand reputation, and extracting insights from textual data sources.

These data analysis methods are instrumental in transforming data into actionable insights, informing decision-making processes, and driving organizational success across various sectors, including business, healthcare, finance, marketing, and research. The selection of a specific method often depends on the nature of the data, the research objectives, and the analytical requirements of the project or organization.

Also Read: Quantitative Data Analysis: Types, Analysis & Examples

Data Analysis Tools

Data analysis tools are essential instruments that facilitate the process of examining, cleaning, transforming, and modeling data to uncover useful information, make informed decisions, and drive strategies. Here are some prominent data analysis tools widely used across various industries:

1) Microsoft Excel:

• Description: A spreadsheet software that offers basic to advanced data analysis features, including pivot tables, data visualization tools, and statistical functions.
• Applications: Data cleaning, basic statistical analysis, visualization, and reporting.

2) R Programming Language :

• Description: An open-source programming language specifically designed for statistical computing and data visualization.
• Applications: Advanced statistical analysis, data manipulation, visualization, and machine learning.

3) Python (with Libraries like Pandas, NumPy, Matplotlib, and Seaborn):

• Description: A versatile programming language with libraries that support data manipulation, analysis, and visualization.
• Applications: Data cleaning, statistical analysis, machine learning, and data visualization.

4) SPSS (Statistical Package for the Social Sciences):

• Description: A comprehensive statistical software suite used for data analysis, data mining, and predictive analytics.
• Applications: Descriptive statistics, hypothesis testing, regression analysis, and advanced analytics.

5) SAS (Statistical Analysis System):

• Description: A software suite used for advanced analytics, multivariate analysis, and predictive modeling.
• Applications: Data management, statistical analysis, predictive modeling, and business intelligence.

6) Tableau:

• Description: A data visualization tool that allows users to create interactive and shareable dashboards and reports.
• Applications: Data visualization , business intelligence , and interactive dashboard creation.

7) Power BI:

• Description: A business analytics tool developed by Microsoft that provides interactive visualizations and business intelligence capabilities.
• Applications: Data visualization, business intelligence, reporting, and dashboard creation.

8) SQL (Structured Query Language) Databases (e.g., MySQL, PostgreSQL, Microsoft SQL Server):

• Description: Database management systems that support data storage, retrieval, and manipulation using SQL queries.
• Applications: Data retrieval, data cleaning, data transformation, and database management.

9) Apache Spark:

• Description: A fast and general-purpose distributed computing system designed for big data processing and analytics.
• Applications: Big data processing, machine learning, data streaming, and real-time analytics.

10) IBM SPSS Modeler:

• Description: A data mining software application used for building predictive models and conducting advanced analytics.
• Applications: Predictive modeling, data mining, statistical analysis, and decision optimization.

These tools serve various purposes and cater to different data analysis needs, from basic statistical analysis and data visualization to advanced analytics, machine learning, and big data processing. The choice of a specific tool often depends on the nature of the data, the complexity of the analysis, and the specific requirements of the project or organization.

Also Read: How to Analyze Survey Data: Methods & Examples

Importance of Data Analysis in Research

The importance of data analysis in research cannot be overstated; it serves as the backbone of any scientific investigation or study. Here are several key reasons why data analysis is crucial in the research process:

• Data analysis helps ensure that the results obtained are valid and reliable. By systematically examining the data, researchers can identify any inconsistencies or anomalies that may affect the credibility of the findings.
• Effective data analysis provides researchers with the necessary information to make informed decisions. By interpreting the collected data, researchers can draw conclusions, make predictions, or formulate recommendations based on evidence rather than intuition or guesswork.
• Data analysis allows researchers to identify patterns, trends, and relationships within the data. This can lead to a deeper understanding of the research topic, enabling researchers to uncover insights that may not be immediately apparent.
• In empirical research, data analysis plays a critical role in testing hypotheses. Researchers collect data to either support or refute their hypotheses, and data analysis provides the tools and techniques to evaluate these hypotheses rigorously.
• Transparent and well-executed data analysis enhances the credibility of research findings. By clearly documenting the data analysis methods and procedures, researchers allow others to replicate the study, thereby contributing to the reproducibility of research findings.
• In fields such as business or healthcare, data analysis helps organizations allocate resources more efficiently. By analyzing data on consumer behavior, market trends, or patient outcomes, organizations can make strategic decisions about resource allocation, budgeting, and planning.
• In public policy and social sciences, data analysis is instrumental in developing and evaluating policies and interventions. By analyzing data on social, economic, or environmental factors, policymakers can assess the effectiveness of existing policies and inform the development of new ones.
• Data analysis allows for continuous improvement in research methods and practices. By analyzing past research projects, identifying areas for improvement, and implementing changes based on data-driven insights, researchers can refine their approaches and enhance the quality of future research endeavors.

However, it is important to remember that mastering these techniques requires practice and continuous learning. That’s why we highly recommend the Data Analytics Course by Physics Wallah . Not only does it cover all the fundamentals of data analysis, but it also provides hands-on experience with various tools such as Excel, Python, and Tableau. Plus, if you use the “ READER ” coupon code at checkout, you can get a special discount on the course.

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Data Analysis Techniques in Research FAQs

What are the 5 techniques for data analysis.

The five techniques for data analysis include: Descriptive Analysis Diagnostic Analysis Predictive Analysis Prescriptive Analysis Qualitative Analysis

What are techniques of data analysis in research?

Techniques of data analysis in research encompass both qualitative and quantitative methods. These techniques involve processes like summarizing raw data, investigating causes of events, forecasting future outcomes, offering recommendations based on predictions, and examining non-numerical data to understand concepts or experiences.

What are the 3 methods of data analysis?

The three primary methods of data analysis are: Qualitative Analysis Quantitative Analysis Mixed-Methods Analysis

What are the four types of data analysis techniques?

The four types of data analysis techniques are: Descriptive Analysis Diagnostic Analysis Predictive Analysis Prescriptive Analysis

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Qualitative Data Analysis Methods 101:

The “big 6” methods + examples.

By: Kerryn Warren (PhD) | Reviewed By: Eunice Rautenbach (D.Tech) | May 2020 (Updated April 2023)

Qualitative data analysis methods. Wow, that’s a mouthful. 

If you’re new to the world of research, qualitative data analysis can look rather intimidating. So much bulky terminology and so many abstract, fluffy concepts. It certainly can be a minefield!

Don’t worry – in this post, we’ll unpack the most popular analysis methods , one at a time, so that you can approach your analysis with confidence and competence – whether that’s for a dissertation, thesis or really any kind of research project.

What (exactly) is qualitative data analysis?

To understand qualitative data analysis, we need to first understand qualitative data – so let’s step back and ask the question, “what exactly is qualitative data?”.

Qualitative data refers to pretty much any data that’s “not numbers” . In other words, it’s not the stuff you measure using a fixed scale or complex equipment, nor do you analyse it using complex statistics or mathematics.

So, if it’s not numbers, what is it?

Words, you guessed? Well… sometimes , yes. Qualitative data can, and often does, take the form of interview transcripts, documents and open-ended survey responses – but it can also involve the interpretation of images and videos. In other words, qualitative isn’t just limited to text-based data.

So, how’s that different from quantitative data, you ask?

Simply put, qualitative research focuses on words, descriptions, concepts or ideas – while quantitative research focuses on numbers and statistics . Qualitative research investigates the “softer side” of things to explore and describe , while quantitative research focuses on the “hard numbers”, to measure differences between variables and the relationships between them. If you’re keen to learn more about the differences between qual and quant, we’ve got a detailed post over here .

So, qualitative analysis is easier than quantitative, right?

Not quite. In many ways, qualitative data can be challenging and time-consuming to analyse and interpret. At the end of your data collection phase (which itself takes a lot of time), you’ll likely have many pages of text-based data or hours upon hours of audio to work through. You might also have subtle nuances of interactions or discussions that have danced around in your mind, or that you scribbled down in messy field notes. All of this needs to work its way into your analysis.

Making sense of all of this is no small task and you shouldn’t underestimate it. Long story short – qualitative analysis can be a lot of work! Of course, quantitative analysis is no piece of cake either, but it’s important to recognise that qualitative analysis still requires a significant investment in terms of time and effort.

Need a helping hand?

In this post, we’ll explore qualitative data analysis by looking at some of the most common analysis methods we encounter. We’re not going to cover every possible qualitative method and we’re not going to go into heavy detail – we’re just going to give you the big picture. That said, we will of course includes links to loads of extra resources so that you can learn more about whichever analysis method interests you.

Without further delay, let’s get into it.

The “Big 6” Qualitative Analysis Methods 

There are many different types of qualitative data analysis, all of which serve different purposes and have unique strengths and weaknesses . We’ll start by outlining the analysis methods and then we’ll dive into the details for each.

The 6 most popular methods (or at least the ones we see at Grad Coach) are:

• Content analysis
• Narrative analysis
• Discourse analysis
• Thematic analysis
• Grounded theory (GT)
• Interpretive phenomenological analysis (IPA)

Let’s take a look at each of them…

QDA Method #1: Qualitative Content Analysis

Content analysis is possibly the most common and straightforward QDA method. At the simplest level, content analysis is used to evaluate patterns within a piece of content (for example, words, phrases or images) or across multiple pieces of content or sources of communication. For example, a collection of newspaper articles or political speeches.

With content analysis, you could, for instance, identify the frequency with which an idea is shared or spoken about – like the number of times a Kardashian is mentioned on Twitter. Or you could identify patterns of deeper underlying interpretations – for instance, by identifying phrases or words in tourist pamphlets that highlight India as an ancient country.

Because content analysis can be used in such a wide variety of ways, it’s important to go into your analysis with a very specific question and goal, or you’ll get lost in the fog. With content analysis, you’ll group large amounts of text into codes , summarise these into categories, and possibly even tabulate the data to calculate the frequency of certain concepts or variables. Because of this, content analysis provides a small splash of quantitative thinking within a qualitative method.

Naturally, while content analysis is widely useful, it’s not without its drawbacks . One of the main issues with content analysis is that it can be very time-consuming , as it requires lots of reading and re-reading of the texts. Also, because of its multidimensional focus on both qualitative and quantitative aspects, it is sometimes accused of losing important nuances in communication.

Content analysis also tends to concentrate on a very specific timeline and doesn’t take into account what happened before or after that timeline. This isn’t necessarily a bad thing though – just something to be aware of. So, keep these factors in mind if you’re considering content analysis. Every analysis method has its limitations , so don’t be put off by these – just be aware of them ! If you’re interested in learning more about content analysis, the video below provides a good starting point.

QDA Method #2: Narrative Analysis 

As the name suggests, narrative analysis is all about listening to people telling stories and analysing what that means . Since stories serve a functional purpose of helping us make sense of the world, we can gain insights into the ways that people deal with and make sense of reality by analysing their stories and the ways they’re told.

You could, for example, use narrative analysis to explore whether how something is being said is important. For instance, the narrative of a prisoner trying to justify their crime could provide insight into their view of the world and the justice system. Similarly, analysing the ways entrepreneurs talk about the struggles in their careers or cancer patients telling stories of hope could provide powerful insights into their mindsets and perspectives . Simply put, narrative analysis is about paying attention to the stories that people tell – and more importantly, the way they tell them.

Of course, the narrative approach has its weaknesses , too. Sample sizes are generally quite small due to the time-consuming process of capturing narratives. Because of this, along with the multitude of social and lifestyle factors which can influence a subject, narrative analysis can be quite difficult to reproduce in subsequent research. This means that it’s difficult to test the findings of some of this research.

Similarly, researcher bias can have a strong influence on the results here, so you need to be particularly careful about the potential biases you can bring into your analysis when using this method. Nevertheless, narrative analysis is still a very useful qualitative analysis method – just keep these limitations in mind and be careful not to draw broad conclusions . If you’re keen to learn more about narrative analysis, the video below provides a great introduction to this qualitative analysis method.

QDA Method #3: Discourse Analysis 

Discourse is simply a fancy word for written or spoken language or debate . So, discourse analysis is all about analysing language within its social context. In other words, analysing language – such as a conversation, a speech, etc – within the culture and society it takes place. For example, you could analyse how a janitor speaks to a CEO, or how politicians speak about terrorism.

To truly understand these conversations or speeches, the culture and history of those involved in the communication are important factors to consider. For example, a janitor might speak more casually with a CEO in a company that emphasises equality among workers. Similarly, a politician might speak more about terrorism if there was a recent terrorist incident in the country.

So, as you can see, by using discourse analysis, you can identify how culture , history or power dynamics (to name a few) have an effect on the way concepts are spoken about. So, if your research aims and objectives involve understanding culture or power dynamics, discourse analysis can be a powerful method.

Because there are many social influences in terms of how we speak to each other, the potential use of discourse analysis is vast . Of course, this also means it’s important to have a very specific research question (or questions) in mind when analysing your data and looking for patterns and themes, or you might land up going down a winding rabbit hole.

Discourse analysis can also be very time-consuming  as you need to sample the data to the point of saturation – in other words, until no new information and insights emerge. But this is, of course, part of what makes discourse analysis such a powerful technique. So, keep these factors in mind when considering this QDA method. Again, if you’re keen to learn more, the video below presents a good starting point.

QDA Method #4: Thematic Analysis

Thematic analysis looks at patterns of meaning in a data set – for example, a set of interviews or focus group transcripts. But what exactly does that… mean? Well, a thematic analysis takes bodies of data (which are often quite large) and groups them according to similarities – in other words, themes . These themes help us make sense of the content and derive meaning from it.

Let’s take a look at an example.

With thematic analysis, you could analyse 100 online reviews of a popular sushi restaurant to find out what patrons think about the place. By reviewing the data, you would then identify the themes that crop up repeatedly within the data – for example, “fresh ingredients” or “friendly wait staff”.

So, as you can see, thematic analysis can be pretty useful for finding out about people’s experiences , views, and opinions . Therefore, if your research aims and objectives involve understanding people’s experience or view of something, thematic analysis can be a great choice.

Since thematic analysis is a bit of an exploratory process, it’s not unusual for your research questions to develop , or even change as you progress through the analysis. While this is somewhat natural in exploratory research, it can also be seen as a disadvantage as it means that data needs to be re-reviewed each time a research question is adjusted. In other words, thematic analysis can be quite time-consuming – but for a good reason. So, keep this in mind if you choose to use thematic analysis for your project and budget extra time for unexpected adjustments.

QDA Method #5: Grounded theory (GT) 

Grounded theory is a powerful qualitative analysis method where the intention is to create a new theory (or theories) using the data at hand, through a series of “ tests ” and “ revisions ”. Strictly speaking, GT is more a research design type than an analysis method, but we’ve included it here as it’s often referred to as a method.

What’s most important with grounded theory is that you go into the analysis with an open mind and let the data speak for itself – rather than dragging existing hypotheses or theories into your analysis. In other words, your analysis must develop from the ground up (hence the name). 

Let’s look at an example of GT in action.

Assume you’re interested in developing a theory about what factors influence students to watch a YouTube video about qualitative analysis. Using Grounded theory , you’d start with this general overarching question about the given population (i.e., graduate students). First, you’d approach a small sample – for example, five graduate students in a department at a university. Ideally, this sample would be reasonably representative of the broader population. You’d interview these students to identify what factors lead them to watch the video.

After analysing the interview data, a general pattern could emerge. For example, you might notice that graduate students are more likely to read a post about qualitative methods if they are just starting on their dissertation journey, or if they have an upcoming test about research methods.

From here, you’ll look for another small sample – for example, five more graduate students in a different department – and see whether this pattern holds true for them. If not, you’ll look for commonalities and adapt your theory accordingly. As this process continues, the theory would develop . As we mentioned earlier, what’s important with grounded theory is that the theory develops from the data – not from some preconceived idea.

So, what are the drawbacks of grounded theory? Well, some argue that there’s a tricky circularity to grounded theory. For it to work, in principle, you should know as little as possible regarding the research question and population, so that you reduce the bias in your interpretation. However, in many circumstances, it’s also thought to be unwise to approach a research question without knowledge of the current literature . In other words, it’s a bit of a “chicken or the egg” situation.

Regardless, grounded theory remains a popular (and powerful) option. Naturally, it’s a very useful method when you’re researching a topic that is completely new or has very little existing research about it, as it allows you to start from scratch and work your way from the ground up .

QDA Method #6:   Interpretive Phenomenological Analysis (IPA)

Interpretive. Phenomenological. Analysis. IPA . Try saying that three times fast…

Let’s just stick with IPA, okay?

IPA is designed to help you understand the personal experiences of a subject (for example, a person or group of people) concerning a major life event, an experience or a situation . This event or experience is the “phenomenon” that makes up the “P” in IPA. Such phenomena may range from relatively common events – such as motherhood, or being involved in a car accident – to those which are extremely rare – for example, someone’s personal experience in a refugee camp. So, IPA is a great choice if your research involves analysing people’s personal experiences of something that happened to them.

It’s important to remember that IPA is subject – centred . In other words, it’s focused on the experiencer . This means that, while you’ll likely use a coding system to identify commonalities, it’s important not to lose the depth of experience or meaning by trying to reduce everything to codes. Also, keep in mind that since your sample size will generally be very small with IPA, you often won’t be able to draw broad conclusions about the generalisability of your findings. But that’s okay as long as it aligns with your research aims and objectives.

Another thing to be aware of with IPA is personal bias . While researcher bias can creep into all forms of research, self-awareness is critically important with IPA, as it can have a major impact on the results. For example, a researcher who was a victim of a crime himself could insert his own feelings of frustration and anger into the way he interprets the experience of someone who was kidnapped. So, if you’re going to undertake IPA, you need to be very self-aware or you could muddy the analysis.

How to choose the right analysis method

In light of all of the qualitative analysis methods we’ve covered so far, you’re probably asking yourself the question, “ How do I choose the right one? ”

Much like all the other methodological decisions you’ll need to make, selecting the right qualitative analysis method largely depends on your research aims, objectives and questions . In other words, the best tool for the job depends on what you’re trying to build. For example:

• Perhaps your research aims to analyse the use of words and what they reveal about the intention of the storyteller and the cultural context of the time.
• Perhaps your research aims to develop an understanding of the unique personal experiences of people that have experienced a certain event, or
• Perhaps your research aims to develop insight regarding the influence of a certain culture on its members.

As you can probably see, each of these research aims are distinctly different , and therefore different analysis methods would be suitable for each one. For example, narrative analysis would likely be a good option for the first aim, while grounded theory wouldn’t be as relevant. 

It’s also important to remember that each method has its own set of strengths, weaknesses and general limitations. No single analysis method is perfect . So, depending on the nature of your research, it may make sense to adopt more than one method (this is called triangulation ). Keep in mind though that this will of course be quite time-consuming.

As we’ve seen, all of the qualitative analysis methods we’ve discussed make use of coding and theme-generating techniques, but the intent and approach of each analysis method differ quite substantially. So, it’s very important to come into your research with a clear intention before you decide which analysis method (or methods) to use.

Start by reviewing your research aims , objectives and research questions to assess what exactly you’re trying to find out – then select a qualitative analysis method that fits. Never pick a method just because you like it or have experience using it – your analysis method (or methods) must align with your broader research aims and objectives.

Let’s recap on QDA methods…

In this post, we looked at six popular qualitative data analysis methods:

• First, we looked at content analysis , a straightforward method that blends a little bit of quant into a primarily qualitative analysis.
• Then we looked at narrative analysis , which is about analysing how stories are told.
• Next up was discourse analysis – which is about analysing conversations and interactions.
• Then we moved on to thematic analysis – which is about identifying themes and patterns.
• From there, we went south with grounded theory – which is about starting from scratch with a specific question and using the data alone to build a theory in response to that question.
• And finally, we looked at IPA – which is about understanding people’s unique experiences of a phenomenon.

Of course, these aren’t the only options when it comes to qualitative data analysis, but they’re a great starting point if you’re dipping your toes into qualitative research for the first time.

If you’re still feeling a bit confused, consider our private coaching service , where we hold your hand through the research process to help you develop your best work.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

87 Comments

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Precise explanation of method.

Hi, may we use 2 data analysis methods in our qualitative research?

Thanks for your comment. Most commonly, one would use one type of analysis method, but it depends on your research aims and objectives.

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Thank nicely explained can I ask is Qualitative content analysis the same as thematic analysis?

Thanks for your comment. No, QCA and thematic are two different types of analysis. This article might help clarify – https://onlinelibrary.wiley.com/doi/10.1111/nhs.12048

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Hi Derek, What other theories/methods would you recommend when the data is a whole speech?

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Very insightful. Please, which of this approach could be used for a research that one is trying to elicit students’ misconceptions in a particular concept ?

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Nicely written especially for novice academic researchers like me! Thank you.

choosing a right method for a paper is always a hard job for a student, this is a useful information, but it would be more useful personally for me, if the author provide me with a little bit more information about the data analysis techniques in type of explanatory research. Can we use qualitative content analysis technique for explanatory research ? or what is the suitable data analysis method for explanatory research in social studies?

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so educative…. but Ijust want to know which method is coding of the qualitative or tallying done?

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Is there a video the captures the practical process of coding using automated applications?

Thanks for the comment. We don’t recommend using automated applications for coding, as they are not sufficiently accurate in our experience.

content analysis can be qualitative research?

THANK YOU VERY MUCH.

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do you have any material on Data collection

What a powerful explanation of the QDA methods. Thank you.

Great explanation both written and Video. i have been using of it on a day to day working of my thesis project in accounting and finance. Thank you very much for your support.

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I certainly hope to hear from you

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Home » Data Analysis – Process, Methods and Types

Data Analysis – Process, Methods and Types

Table of Contents

Data Analysis

Definition:

Data analysis refers to the process of inspecting, cleaning, transforming, and modeling data with the goal of discovering useful information, drawing conclusions, and supporting decision-making. It involves applying various statistical and computational techniques to interpret and derive insights from large datasets. The ultimate aim of data analysis is to convert raw data into actionable insights that can inform business decisions, scientific research, and other endeavors.

Data Analysis Process

The following are step-by-step guides to the data analysis process:

Define the Problem

The first step in data analysis is to clearly define the problem or question that needs to be answered. This involves identifying the purpose of the analysis, the data required, and the intended outcome.

Collect the Data

The next step is to collect the relevant data from various sources. This may involve collecting data from surveys, databases, or other sources. It is important to ensure that the data collected is accurate, complete, and relevant to the problem being analyzed.

Clean and Organize the Data

Once the data has been collected, it needs to be cleaned and organized. This involves removing any errors or inconsistencies in the data, filling in missing values, and ensuring that the data is in a format that can be easily analyzed.

Analyze the Data

The next step is to analyze the data using various statistical and analytical techniques. This may involve identifying patterns in the data, conducting statistical tests, or using machine learning algorithms to identify trends and insights.

Interpret the Results

After analyzing the data, the next step is to interpret the results. This involves drawing conclusions based on the analysis and identifying any significant findings or trends.

Communicate the Findings

Once the results have been interpreted, they need to be communicated to stakeholders. This may involve creating reports, visualizations, or presentations to effectively communicate the findings and recommendations.

Take Action

The final step in the data analysis process is to take action based on the findings. This may involve implementing new policies or procedures, making strategic decisions, or taking other actions based on the insights gained from the analysis.

Types of Data Analysis

Types of Data Analysis are as follows:

Descriptive Analysis

This type of analysis involves summarizing and describing the main characteristics of a dataset, such as the mean, median, mode, standard deviation, and range.

Inferential Analysis

This type of analysis involves making inferences about a population based on a sample. Inferential analysis can help determine whether a certain relationship or pattern observed in a sample is likely to be present in the entire population.

Diagnostic Analysis

This type of analysis involves identifying and diagnosing problems or issues within a dataset. Diagnostic analysis can help identify outliers, errors, missing data, or other anomalies in the dataset.

Predictive Analysis

This type of analysis involves using statistical models and algorithms to predict future outcomes or trends based on historical data. Predictive analysis can help businesses and organizations make informed decisions about the future.

Prescriptive Analysis

This type of analysis involves recommending a course of action based on the results of previous analyses. Prescriptive analysis can help organizations make data-driven decisions about how to optimize their operations, products, or services.

Exploratory Analysis

This type of analysis involves exploring the relationships and patterns within a dataset to identify new insights and trends. Exploratory analysis is often used in the early stages of research or data analysis to generate hypotheses and identify areas for further investigation.

Data Analysis Methods

Data Analysis Methods are as follows:

Statistical Analysis

This method involves the use of mathematical models and statistical tools to analyze and interpret data. It includes measures of central tendency, correlation analysis, regression analysis, hypothesis testing, and more.

Machine Learning

This method involves the use of algorithms to identify patterns and relationships in data. It includes supervised and unsupervised learning, classification, clustering, and predictive modeling.

Data Mining

This method involves using statistical and machine learning techniques to extract information and insights from large and complex datasets.

Text Analysis

This method involves using natural language processing (NLP) techniques to analyze and interpret text data. It includes sentiment analysis, topic modeling, and entity recognition.

Network Analysis

This method involves analyzing the relationships and connections between entities in a network, such as social networks or computer networks. It includes social network analysis and graph theory.

Time Series Analysis

This method involves analyzing data collected over time to identify patterns and trends. It includes forecasting, decomposition, and smoothing techniques.

Spatial Analysis

This method involves analyzing geographic data to identify spatial patterns and relationships. It includes spatial statistics, spatial regression, and geospatial data visualization.

Data Visualization

This method involves using graphs, charts, and other visual representations to help communicate the findings of the analysis. It includes scatter plots, bar charts, heat maps, and interactive dashboards.

Qualitative Analysis

This method involves analyzing non-numeric data such as interviews, observations, and open-ended survey responses. It includes thematic analysis, content analysis, and grounded theory.

Multi-criteria Decision Analysis

This method involves analyzing multiple criteria and objectives to support decision-making. It includes techniques such as the analytical hierarchy process, TOPSIS, and ELECTRE.

Data Analysis Tools

There are various data analysis tools available that can help with different aspects of data analysis. Below is a list of some commonly used data analysis tools:

• Microsoft Excel: A widely used spreadsheet program that allows for data organization, analysis, and visualization.
• SQL : A programming language used to manage and manipulate relational databases.
• R : An open-source programming language and software environment for statistical computing and graphics.
• Python : A general-purpose programming language that is widely used in data analysis and machine learning.
• Tableau : A data visualization software that allows for interactive and dynamic visualizations of data.
• SAS : A statistical analysis software used for data management, analysis, and reporting.
• SPSS : A statistical analysis software used for data analysis, reporting, and modeling.
• Matlab : A numerical computing software that is widely used in scientific research and engineering.
• RapidMiner : A data science platform that offers a wide range of data analysis and machine learning tools.

Applications of Data Analysis

Data analysis has numerous applications across various fields. Below are some examples of how data analysis is used in different fields:

• Business : Data analysis is used to gain insights into customer behavior, market trends, and financial performance. This includes customer segmentation, sales forecasting, and market research.
• Healthcare : Data analysis is used to identify patterns and trends in patient data, improve patient outcomes, and optimize healthcare operations. This includes clinical decision support, disease surveillance, and healthcare cost analysis.
• Education : Data analysis is used to measure student performance, evaluate teaching effectiveness, and improve educational programs. This includes assessment analytics, learning analytics, and program evaluation.
• Finance : Data analysis is used to monitor and evaluate financial performance, identify risks, and make investment decisions. This includes risk management, portfolio optimization, and fraud detection.
• Government : Data analysis is used to inform policy-making, improve public services, and enhance public safety. This includes crime analysis, disaster response planning, and social welfare program evaluation.
• Sports : Data analysis is used to gain insights into athlete performance, improve team strategy, and enhance fan engagement. This includes player evaluation, scouting analysis, and game strategy optimization.
• Marketing : Data analysis is used to measure the effectiveness of marketing campaigns, understand customer behavior, and develop targeted marketing strategies. This includes customer segmentation, marketing attribution analysis, and social media analytics.
• Environmental science : Data analysis is used to monitor and evaluate environmental conditions, assess the impact of human activities on the environment, and develop environmental policies. This includes climate modeling, ecological forecasting, and pollution monitoring.

When to Use Data Analysis

Data analysis is useful when you need to extract meaningful insights and information from large and complex datasets. It is a crucial step in the decision-making process, as it helps you understand the underlying patterns and relationships within the data, and identify potential areas for improvement or opportunities for growth.

Here are some specific scenarios where data analysis can be particularly helpful:

• Problem-solving : When you encounter a problem or challenge, data analysis can help you identify the root cause and develop effective solutions.
• Optimization : Data analysis can help you optimize processes, products, or services to increase efficiency, reduce costs, and improve overall performance.
• Prediction: Data analysis can help you make predictions about future trends or outcomes, which can inform strategic planning and decision-making.
• Performance evaluation : Data analysis can help you evaluate the performance of a process, product, or service to identify areas for improvement and potential opportunities for growth.
• Risk assessment : Data analysis can help you assess and mitigate risks, whether it is financial, operational, or related to safety.
• Market research : Data analysis can help you understand customer behavior and preferences, identify market trends, and develop effective marketing strategies.
• Quality control: Data analysis can help you ensure product quality and customer satisfaction by identifying and addressing quality issues.

Purpose of Data Analysis

The primary purposes of data analysis can be summarized as follows:

• To gain insights: Data analysis allows you to identify patterns and trends in data, which can provide valuable insights into the underlying factors that influence a particular phenomenon or process.
• To inform decision-making: Data analysis can help you make informed decisions based on the information that is available. By analyzing data, you can identify potential risks, opportunities, and solutions to problems.
• To improve performance: Data analysis can help you optimize processes, products, or services by identifying areas for improvement and potential opportunities for growth.
• To measure progress: Data analysis can help you measure progress towards a specific goal or objective, allowing you to track performance over time and adjust your strategies accordingly.
• To identify new opportunities: Data analysis can help you identify new opportunities for growth and innovation by identifying patterns and trends that may not have been visible before.

Examples of Data Analysis

Some Examples of Data Analysis are as follows:

• Social Media Monitoring: Companies use data analysis to monitor social media activity in real-time to understand their brand reputation, identify potential customer issues, and track competitors. By analyzing social media data, businesses can make informed decisions on product development, marketing strategies, and customer service.
• Financial Trading: Financial traders use data analysis to make real-time decisions about buying and selling stocks, bonds, and other financial instruments. By analyzing real-time market data, traders can identify trends and patterns that help them make informed investment decisions.
• Traffic Monitoring : Cities use data analysis to monitor traffic patterns and make real-time decisions about traffic management. By analyzing data from traffic cameras, sensors, and other sources, cities can identify congestion hotspots and make changes to improve traffic flow.
• Healthcare Monitoring: Healthcare providers use data analysis to monitor patient health in real-time. By analyzing data from wearable devices, electronic health records, and other sources, healthcare providers can identify potential health issues and provide timely interventions.
• Online Advertising: Online advertisers use data analysis to make real-time decisions about advertising campaigns. By analyzing data on user behavior and ad performance, advertisers can make adjustments to their campaigns to improve their effectiveness.
• Sports Analysis : Sports teams use data analysis to make real-time decisions about strategy and player performance. By analyzing data on player movement, ball position, and other variables, coaches can make informed decisions about substitutions, game strategy, and training regimens.
• Energy Management : Energy companies use data analysis to monitor energy consumption in real-time. By analyzing data on energy usage patterns, companies can identify opportunities to reduce energy consumption and improve efficiency.

Characteristics of Data Analysis

Characteristics of Data Analysis are as follows:

• Objective : Data analysis should be objective and based on empirical evidence, rather than subjective assumptions or opinions.
• Systematic : Data analysis should follow a systematic approach, using established methods and procedures for collecting, cleaning, and analyzing data.
• Accurate : Data analysis should produce accurate results, free from errors and bias. Data should be validated and verified to ensure its quality.
• Relevant : Data analysis should be relevant to the research question or problem being addressed. It should focus on the data that is most useful for answering the research question or solving the problem.
• Comprehensive : Data analysis should be comprehensive and consider all relevant factors that may affect the research question or problem.
• Timely : Data analysis should be conducted in a timely manner, so that the results are available when they are needed.
• Reproducible : Data analysis should be reproducible, meaning that other researchers should be able to replicate the analysis using the same data and methods.
• Communicable : Data analysis should be communicated clearly and effectively to stakeholders and other interested parties. The results should be presented in a way that is understandable and useful for decision-making.

Advantages of Data Analysis

Advantages of Data Analysis are as follows:

• Better decision-making: Data analysis helps in making informed decisions based on facts and evidence, rather than intuition or guesswork.
• Improved efficiency: Data analysis can identify inefficiencies and bottlenecks in business processes, allowing organizations to optimize their operations and reduce costs.
• Increased accuracy: Data analysis helps to reduce errors and bias, providing more accurate and reliable information.
• Better customer service: Data analysis can help organizations understand their customers better, allowing them to provide better customer service and improve customer satisfaction.
• Competitive advantage: Data analysis can provide organizations with insights into their competitors, allowing them to identify areas where they can gain a competitive advantage.
• Identification of trends and patterns : Data analysis can identify trends and patterns in data that may not be immediately apparent, helping organizations to make predictions and plan for the future.
• Improved risk management : Data analysis can help organizations identify potential risks and take proactive steps to mitigate them.
• Innovation: Data analysis can inspire innovation and new ideas by revealing new opportunities or previously unknown correlations in data.

Limitations of Data Analysis

• Data quality: The quality of data can impact the accuracy and reliability of analysis results. If data is incomplete, inconsistent, or outdated, the analysis may not provide meaningful insights.
• Limited scope: Data analysis is limited by the scope of the data available. If data is incomplete or does not capture all relevant factors, the analysis may not provide a complete picture.
• Human error : Data analysis is often conducted by humans, and errors can occur in data collection, cleaning, and analysis.
• Cost : Data analysis can be expensive, requiring specialized tools, software, and expertise.
• Time-consuming : Data analysis can be time-consuming, especially when working with large datasets or conducting complex analyses.
• Overreliance on data: Data analysis should be complemented with human intuition and expertise. Overreliance on data can lead to a lack of creativity and innovation.
• Privacy concerns: Data analysis can raise privacy concerns if personal or sensitive information is used without proper consent or security measures.

About the author

Muhammad Hassan

Researcher, Academic Writer, Web developer

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Home Market Research

Qualitative Data Analysis: What is it, Methods + Examples

In a world rich with information and narrative, understanding the deeper layers of human experiences requires a unique vision that goes beyond numbers and figures. This is where the power of qualitative data analysis comes to light.

In this blog, we’ll learn about qualitative data analysis, explore its methods, and provide real-life examples showcasing its power in uncovering insights.

What is Qualitative Data Analysis?

Qualitative data analysis is a systematic process of examining non-numerical data to extract meaning, patterns, and insights.

In contrast to quantitative analysis, which focuses on numbers and statistical metrics, the qualitative study focuses on the qualitative aspects of data, such as text, images, audio, and videos. It seeks to understand every aspect of human experiences, perceptions, and behaviors by examining the data’s richness.

Companies frequently conduct this analysis on customer feedback. You can collect qualitative data from reviews, complaints, chat messages, interactions with support centers, customer interviews, case notes, or even social media comments. This kind of data holds the key to understanding customer sentiments and preferences in a way that goes beyond mere numbers.

Importance of Qualitative Data Analysis

Qualitative data analysis plays a crucial role in your research and decision-making process across various disciplines. Let’s explore some key reasons that underline the significance of this analysis:

In-Depth Understanding

It enables you to explore complex and nuanced aspects of a phenomenon, delving into the ‘how’ and ‘why’ questions. This method provides you with a deeper understanding of human behavior, experiences, and contexts that quantitative approaches might not capture fully.

Contextual Insight

You can use this analysis to give context to numerical data. It will help you understand the circumstances and conditions that influence participants’ thoughts, feelings, and actions. This contextual insight becomes essential for generating comprehensive explanations.

Theory Development

You can generate or refine hypotheses via qualitative data analysis. As you analyze the data attentively, you can form hypotheses, concepts, and frameworks that will drive your future research and contribute to theoretical advances.

Participant Perspectives

When performing qualitative research, you can highlight participant voices and opinions. This approach is especially useful for understanding marginalized or underrepresented people, as it allows them to communicate their experiences and points of view.

Exploratory Research

The analysis is frequently used at the exploratory stage of your project. It assists you in identifying important variables, developing research questions, and designing quantitative studies that will follow.

Types of Qualitative Data

When conducting qualitative research, you can use several qualitative data collection methods , and here you will come across many sorts of qualitative data that can provide you with unique insights into your study topic. These data kinds add new views and angles to your understanding and analysis.

Interviews and Focus Groups

Interviews and focus groups will be among your key methods for gathering qualitative data. Interviews are one-on-one talks in which participants can freely share their thoughts, experiences, and opinions.

Focus groups, on the other hand, are discussions in which members interact with one another, resulting in dynamic exchanges of ideas. Both methods provide rich qualitative data and direct access to participant perspectives.

Observations and Field Notes

Observations and field notes are another useful sort of qualitative data. You can immerse yourself in the research environment through direct observation, carefully documenting behaviors, interactions, and contextual factors.

These observations will be recorded in your field notes, providing a complete picture of the environment and the behaviors you’re researching. This data type is especially important for comprehending behavior in their natural setting.

Textual and Visual Data

Textual and visual data include a wide range of resources that can be qualitatively analyzed. Documents, written narratives, and transcripts from various sources, such as interviews or speeches, are examples of textual data.

Photographs, films, and even artwork provide a visual layer to your research. These forms of data allow you to investigate what is spoken and the underlying emotions, details, and symbols expressed by language or pictures.

When to Choose Qualitative Data Analysis over Quantitative Data Analysis

As you begin your research journey, understanding why the analysis of qualitative data is important will guide your approach to understanding complex events. If you analyze qualitative data, it will provide new insights that complement quantitative methodologies, which will give you a broader understanding of your study topic.

It is critical to know when to use qualitative analysis over quantitative procedures. You can prefer qualitative data analysis when:

• Complexity Reigns: When your research questions involve deep human experiences, motivations, or emotions, qualitative research excels at revealing these complexities.
• Exploration is Key: Qualitative analysis is ideal for exploratory research. It will assist you in understanding a new or poorly understood topic before formulating quantitative hypotheses.
• Context Matters: If you want to understand how context affects behaviors or results, qualitative data analysis provides the depth needed to grasp these relationships.
• Unanticipated Findings: When your study provides surprising new viewpoints or ideas, qualitative analysis helps you to delve deeply into these emerging themes.
• Subjective Interpretation is Vital: When it comes to understanding people’s subjective experiences and interpretations, qualitative data analysis is the way to go.

You can make informed decisions regarding the right approach for your research objectives if you understand the importance of qualitative analysis and recognize the situations where it shines.

Qualitative Data Analysis Methods and Examples

Exploring various qualitative data analysis methods will provide you with a wide collection for making sense of your research findings. Once the data has been collected, you can choose from several analysis methods based on your research objectives and the data type you’ve collected.

There are five main methods for analyzing qualitative data. Each method takes a distinct approach to identifying patterns, themes, and insights within your qualitative data. They are:

Method 1: Content Analysis

Content analysis is a methodical technique for analyzing textual or visual data in a structured manner. In this method, you will categorize qualitative data by splitting it into manageable pieces and assigning the manual coding process to these units.

As you go, you’ll notice ongoing codes and designs that will allow you to conclude the content. This method is very beneficial for detecting common ideas, concepts, or themes in your data without losing the context.

Steps to Do Content Analysis

Follow these steps when conducting content analysis:

• Collect and Immerse: Begin by collecting the necessary textual or visual data. Immerse yourself in this data to fully understand its content, context, and complexities.
• Assign Codes and Categories: Assign codes to relevant data sections that systematically represent major ideas or themes. Arrange comparable codes into groups that cover the major themes.
• Analyze and Interpret: Develop a structured framework from the categories and codes. Then, evaluate the data in the context of your research question, investigate relationships between categories, discover patterns, and draw meaning from these connections.

Benefits & Challenges

There are various advantages to using content analysis:

• Structured Approach: It offers a systematic approach to dealing with large data sets and ensures consistency throughout the research.
• Objective Insights: This method promotes objectivity, which helps to reduce potential biases in your study.
• Pattern Discovery: Content analysis can help uncover hidden trends, themes, and patterns that are not always obvious.
• Versatility: You can apply content analysis to various data formats, including text, internet content, images, etc.

However, keep in mind the challenges that arise:

• Subjectivity: Even with the best attempts, a certain bias may remain in coding and interpretation.
• Complexity: Analyzing huge data sets requires time and great attention to detail.
• Contextual Nuances: Content analysis may not capture all of the contextual richness that qualitative data analysis highlights.

Example of Content Analysis

Suppose you’re conducting market research and looking at customer feedback on a product. As you collect relevant data and analyze feedback, you’ll see repeating codes like “price,” “quality,” “customer service,” and “features.” These codes are organized into categories such as “positive reviews,” “negative reviews,” and “suggestions for improvement.”

According to your findings, themes such as “price” and “customer service” stand out and show that pricing and customer service greatly impact customer satisfaction. This example highlights the power of content analysis for obtaining significant insights from large textual data collections.

Method 2: Thematic Analysis

Thematic analysis is a well-structured procedure for identifying and analyzing recurring themes in your data. As you become more engaged in the data, you’ll generate codes or short labels representing key concepts. These codes are then organized into themes, providing a consistent framework for organizing and comprehending the substance of the data.

The analysis allows you to organize complex narratives and perspectives into meaningful categories, which will allow you to identify connections and patterns that may not be visible at first.

Steps to Do Thematic Analysis

Follow these steps when conducting a thematic analysis:

• Code and Group: Start by thoroughly examining the data and giving initial codes that identify the segments. To create initial themes, combine relevant codes.
• Code and Group: Begin by engaging yourself in the data, assigning first codes to notable segments. To construct basic themes, group comparable codes together.
• Analyze and Report: Analyze the data within each theme to derive relevant insights. Organize the topics into a consistent structure and explain your findings, along with data extracts that represent each theme.

Thematic analysis has various benefits:

• Structured Exploration: It is a method for identifying patterns and themes in complex qualitative data.
• Comprehensive knowledge: Thematic analysis promotes an in-depth understanding of the complications and meanings of the data.
• Application Flexibility: This method can be customized to various research situations and data kinds.

However, challenges may arise, such as:

• Interpretive Nature: Interpreting qualitative data in thematic analysis is vital, and it is critical to manage researcher bias.
• Time-consuming: The study can be time-consuming, especially with large data sets.
• Subjectivity: The selection of codes and topics might be subjective.

Example of Thematic Analysis

Assume you’re conducting a thematic analysis on job satisfaction interviews. Following your immersion in the data, you assign initial codes such as “work-life balance,” “career growth,” and “colleague relationships.” As you organize these codes, you’ll notice themes develop, such as “Factors Influencing Job Satisfaction” and “Impact on Work Engagement.”

Further investigation reveals the tales and experiences included within these themes and provides insights into how various elements influence job satisfaction. This example demonstrates how thematic analysis can reveal meaningful patterns and insights in qualitative data.

Method 3: Narrative Analysis

The narrative analysis involves the narratives that people share. You’ll investigate the histories in your data, looking at how stories are created and the meanings they express. This method is excellent for learning how people make sense of their experiences through narrative.

Steps to Do Narrative Analysis

The following steps are involved in narrative analysis:

• Gather and Analyze: Start by collecting narratives, such as first-person tales, interviews, or written accounts. Analyze the stories, focusing on the plot, feelings, and characters.
• Find Themes: Look for recurring themes or patterns in various narratives. Think about the similarities and differences between these topics and personal experiences.
• Interpret and Extract Insights: Contextualize the narratives within their larger context. Accept the subjective nature of each narrative and analyze the narrator’s voice and style. Extract insights from the tales by diving into the emotions, motivations, and implications communicated by the stories.

There are various advantages to narrative analysis:

• Deep Exploration: It lets you look deeply into people’s personal experiences and perspectives.
• Human-Centered: This method prioritizes the human perspective, allowing individuals to express themselves.

However, difficulties may arise, such as:

• Interpretive Complexity: Analyzing narratives requires dealing with the complexities of meaning and interpretation.
• Time-consuming: Because of the richness and complexities of tales, working with them can be time-consuming.

Example of Narrative Analysis

Assume you’re conducting narrative analysis on refugee interviews. As you read the stories, you’ll notice common themes of toughness, loss, and hope. The narratives provide insight into the obstacles that refugees face, their strengths, and the dreams that guide them.

The analysis can provide a deeper insight into the refugees’ experiences and the broader social context they navigate by examining the narratives’ emotional subtleties and underlying meanings. This example highlights how narrative analysis can reveal important insights into human stories.

Method 4: Grounded Theory Analysis

Grounded theory analysis is an iterative and systematic approach that allows you to create theories directly from data without being limited by pre-existing hypotheses. With an open mind, you collect data and generate early codes and labels that capture essential ideas or concepts within the data.

As you progress, you refine these codes and increasingly connect them, eventually developing a theory based on the data. Grounded theory analysis is a dynamic process for developing new insights and hypotheses based on details in your data.

Steps to Do Grounded Theory Analysis

Grounded theory analysis requires the following steps:

• Initial Coding: First, immerse yourself in the data, producing initial codes that represent major concepts or patterns.
• Categorize and Connect: Using axial coding, organize the initial codes, which establish relationships and connections between topics.
• Build the Theory: Focus on creating a core category that connects the codes and themes. Regularly refine the theory by comparing and integrating new data, ensuring that it evolves organically from the data.

Grounded theory analysis has various benefits:

• Theory Generation: It provides a one-of-a-kind opportunity to generate hypotheses straight from data and promotes new insights.
• In-depth Understanding: The analysis allows you to deeply analyze the data and reveal complex relationships and patterns.
• Flexible Process: This method is customizable and ongoing, which allows you to enhance your research as you collect additional data.

However, challenges might arise with:

• Time and Resources: Because grounded theory analysis is a continuous process, it requires a large commitment of time and resources.
• Theoretical Development: Creating a grounded theory involves a thorough understanding of qualitative data analysis software and theoretical concepts.
• Interpretation of Complexity: Interpreting and incorporating a newly developed theory into existing literature can be intellectually hard.

Example of Grounded Theory Analysis

Assume you’re performing a grounded theory analysis on workplace collaboration interviews. As you open code the data, you will discover notions such as “communication barriers,” “team dynamics,” and “leadership roles.” Axial coding demonstrates links between these notions, emphasizing the significance of efficient communication in developing collaboration.

You create the core “Integrated Communication Strategies” category through selective coding, which unifies new topics.

This theory-driven category serves as the framework for understanding how numerous aspects contribute to effective team collaboration. This example shows how grounded theory analysis allows you to generate a theory directly from the inherent nature of the data.

Method 5: Discourse Analysis

Discourse analysis focuses on language and communication. You’ll look at how language produces meaning and how it reflects power relations, identities, and cultural influences. This strategy examines what is said and how it is said; the words, phrasing, and larger context of communication.

The analysis is precious when investigating power dynamics, identities, and cultural influences encoded in language. By evaluating the language used in your data, you can identify underlying assumptions, cultural standards, and how individuals negotiate meaning through communication.

Steps to Do Discourse Analysis

Conducting discourse analysis entails the following steps:

• Select Discourse: For analysis, choose language-based data such as texts, speeches, or media content.
• Analyze Language: Immerse yourself in the conversation, examining language choices, metaphors, and underlying assumptions.
• Discover Patterns: Recognize the dialogue’s reoccurring themes, ideologies, and power dynamics. To fully understand the effects of these patterns, put them in their larger context.

There are various advantages of using discourse analysis:

• Understanding Language: It provides an extensive understanding of how language builds meaning and influences perceptions.
• Uncovering Power Dynamics: The analysis reveals how power dynamics appear via language.
• Cultural Insights: This method identifies cultural norms, beliefs, and ideologies stored in communication.

However, the following challenges may arise:

• Complexity of Interpretation: Language analysis involves navigating multiple levels of nuance and interpretation.
• Subjectivity: Interpretation can be subjective, so controlling researcher bias is important.
• Time-Intensive: Discourse analysis can take a lot of time because careful linguistic study is required in this analysis.

Example of Discourse Analysis

Consider doing discourse analysis on media coverage of a political event. You notice repeating linguistic patterns in news articles that depict the event as a conflict between opposing parties. Through deconstruction, you can expose how this framing supports particular ideologies and power relations.

You can illustrate how language choices influence public perceptions and contribute to building the narrative around the event by analyzing the speech within the broader political and social context. This example shows how discourse analysis can reveal hidden power dynamics and cultural influences on communication.

How to do Qualitative Data Analysis with the QuestionPro Research suite?

QuestionPro is a popular survey and research platform that offers tools for collecting and analyzing qualitative and quantitative data. Follow these general steps for conducting qualitative data analysis using the QuestionPro Research Suite:

• Collect Qualitative Data: Set up your survey to capture qualitative responses. It might involve open-ended questions, text boxes, or comment sections where participants can provide detailed responses.
• Export Qualitative Responses: Export the responses once you’ve collected qualitative data through your survey. QuestionPro typically allows you to export survey data in various formats, such as Excel or CSV.
• Prepare Data for Analysis: Review the exported data and clean it if necessary. Remove irrelevant or duplicate entries to ensure your data is ready for analysis.
• Code and Categorize Responses: Segment and label data, letting new patterns emerge naturally, then develop categories through axial coding to structure the analysis.
• Identify Themes: Analyze the coded responses to identify recurring themes, patterns, and insights. Look for similarities and differences in participants’ responses.
• Generate Reports and Visualizations: Utilize the reporting features of QuestionPro to create visualizations, charts, and graphs that help communicate the themes and findings from your qualitative research.
• Interpret and Draw Conclusions: Interpret the themes and patterns you’ve identified in the qualitative data. Consider how these findings answer your research questions or provide insights into your study topic.
• Integrate with Quantitative Data (if applicable): If you’re also conducting quantitative research using QuestionPro, consider integrating your qualitative findings with quantitative results to provide a more comprehensive understanding.

Qualitative data analysis is vital in uncovering various human experiences, views, and stories. If you’re ready to transform your research journey and apply the power of qualitative analysis, now is the moment to do it. Book a demo with QuestionPro today and begin your journey of exploration.

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Welcome to the community

Qualitative Data Analysis: Step-by-Step Guide (Manual vs. Automatic)

When we conduct qualitative methods of research, need to explain changes in metrics or understand people's opinions, we always turn to qualitative data. Qualitative data is typically generated through:

• Interview transcripts
• Surveys with open-ended questions
• Contact center transcripts
• Texts and documents
• Audio and video recordings
• Observational notes

Compared to quantitative data, which captures structured information, qualitative data is unstructured and has more depth. It can answer our questions, can help formulate hypotheses and build understanding.

It's important to understand the differences between quantitative data & qualitative data . But unfortunately, analyzing qualitative data is difficult. While tools like Excel, Tableau and PowerBI crunch and visualize quantitative data with ease, there are a limited number of mainstream tools for analyzing qualitative data . The majority of qualitative data analysis still happens manually.

That said, there are two new trends that are changing this. First, there are advances in natural language processing (NLP) which is focused on understanding human language. Second, there is an explosion of user-friendly software designed for both researchers and businesses. Both help automate the qualitative data analysis process.

In this post we want to teach you how to conduct a successful qualitative data analysis. There are two primary qualitative data analysis methods; manual & automatic. We will teach you how to conduct the analysis manually, and also, automatically using software solutions powered by NLP. We’ll guide you through the steps to conduct a manual analysis, and look at what is involved and the role technology can play in automating this process.

More businesses are switching to fully-automated analysis of qualitative customer data because it is cheaper, faster, and just as accurate. Primarily, businesses purchase subscriptions to feedback analytics platforms so that they can understand customer pain points and sentiment.

We’ll take you through 5 steps to conduct a successful qualitative data analysis. Within each step we will highlight the key difference between the manual, and automated approach of qualitative researchers. Here's an overview of the steps:

The 5 steps to doing qualitative data analysis

• Gathering and collecting your qualitative data
• Organizing and connecting into your qualitative data
• Coding your qualitative data
• Analyzing the qualitative data for insights
• Reporting on the insights derived from your analysis

What is Qualitative Data Analysis?

Qualitative data analysis is a process of gathering, structuring and interpreting qualitative data to understand what it represents.

Qualitative data is non-numerical and unstructured. Qualitative data generally refers to text, such as open-ended responses to survey questions or user interviews, but also includes audio, photos and video.

Businesses often perform qualitative data analysis on customer feedback. And within this context, qualitative data generally refers to verbatim text data collected from sources such as reviews, complaints, chat messages, support centre interactions, customer interviews, case notes or social media comments.

How is qualitative data analysis different from quantitative data analysis?

Understanding the differences between quantitative & qualitative data is important. When it comes to analyzing data, Qualitative Data Analysis serves a very different role to Quantitative Data Analysis. But what sets them apart?

Qualitative Data Analysis dives into the stories hidden in non-numerical data such as interviews, open-ended survey answers, or notes from observations. It uncovers the ‘whys’ and ‘hows’ giving a deep understanding of people’s experiences and emotions.

Quantitative Data Analysis on the other hand deals with numerical data, using statistics to measure differences, identify preferred options, and pinpoint root causes of issues.  It steps back to address questions like "how many" or "what percentage" to offer broad insights we can apply to larger groups.

In short, Qualitative Data Analysis is like a microscope,  helping us understand specific detail. Quantitative Data Analysis is like the telescope, giving us a broader perspective. Both are important, working together to decode data for different objectives.

Qualitative Data Analysis methods

Once all the data has been captured, there are a variety of analysis techniques available and the choice is determined by your specific research objectives and the kind of data you’ve gathered.  Common qualitative data analysis methods include:

Content Analysis

This is a popular approach to qualitative data analysis. Other qualitative analysis techniques may fit within the broad scope of content analysis. Thematic analysis is a part of the content analysis.  Content analysis is used to identify the patterns that emerge from text, by grouping content into words, concepts, and themes. Content analysis is useful to quantify the relationship between all of the grouped content. The Columbia School of Public Health has a detailed breakdown of content analysis .

Narrative Analysis

Narrative analysis focuses on the stories people tell and the language they use to make sense of them.  It is particularly useful in qualitative research methods where customer stories are used to get a deep understanding of customers’ perspectives on a specific issue. A narrative analysis might enable us to summarize the outcomes of a focused case study.

Discourse Analysis

Discourse analysis is used to get a thorough understanding of the political, cultural and power dynamics that exist in specific situations.  The focus of discourse analysis here is on the way people express themselves in different social contexts. Discourse analysis is commonly used by brand strategists who hope to understand why a group of people feel the way they do about a brand or product.

Thematic Analysis

Thematic analysis is used to deduce the meaning behind the words people use. This is accomplished by discovering repeating themes in text. These meaningful themes reveal key insights into data and can be quantified, particularly when paired with sentiment analysis . Often, the outcome of thematic analysis is a code frame that captures themes in terms of codes, also called categories. So the process of thematic analysis is also referred to as “coding”. A common use-case for thematic analysis in companies is analysis of customer feedback.

Grounded Theory

Grounded theory is a useful approach when little is known about a subject. Grounded theory starts by formulating a theory around a single data case. This means that the theory is “grounded”. Grounded theory analysis is based on actual data, and not entirely speculative. Then additional cases can be examined to see if they are relevant and can add to the original grounded theory.

Challenges of Qualitative Data Analysis

While Qualitative Data Analysis offers rich insights, it comes with its challenges. Each unique QDA method has its unique hurdles. Let’s take a look at the challenges researchers and analysts might face, depending on the chosen method.

• Time and Effort (Narrative Analysis): Narrative analysis, which focuses on personal stories, demands patience. Sifting through lengthy narratives to find meaningful insights can be time-consuming, requires dedicated effort.
• Being Objective (Grounded Theory): Grounded theory, building theories from data, faces the challenges of personal biases. Staying objective while interpreting data is crucial, ensuring conclusions are rooted in the data itself.
• Complexity (Thematic Analysis): Thematic analysis involves identifying themes within data, a process that can be intricate. Categorizing and understanding themes can be complex, especially when each piece of data varies in context and structure. Thematic Analysis software can simplify this process.
• Generalizing Findings (Narrative Analysis): Narrative analysis, dealing with individual stories, makes drawing broad challenging. Extending findings from a single narrative to a broader context requires careful consideration.
• Managing Data (Thematic Analysis): Thematic analysis involves organizing and managing vast amounts of unstructured data, like interview transcripts. Managing this can be a hefty task, requiring effective data management strategies.
• Skill Level (Grounded Theory): Grounded theory demands specific skills to build theories from the ground up. Finding or training analysts with these skills poses a challenge, requiring investment in building expertise.

Benefits of qualitative data analysis

Qualitative Data Analysis (QDA) is like a versatile toolkit, offering a tailored approach to understanding your data. The benefits it offers are as diverse as the methods. Let’s explore why choosing the right method matters.

• Tailored Methods for Specific Needs: QDA isn't one-size-fits-all. Depending on your research objectives and the type of data at hand, different methods offer unique benefits. If you want emotive customer stories, narrative analysis paints a strong picture. When you want to explain a score, thematic analysis reveals insightful patterns
• Flexibility with Thematic Analysis: thematic analysis is like a chameleon in the toolkit of QDA. It adapts well to different types of data and research objectives, making it a top choice for any qualitative analysis.
• Deeper Understanding, Better Products: QDA helps you dive into people's thoughts and feelings. This deep understanding helps you build products and services that truly matches what people want, ensuring satisfied customers
• Finding the Unexpected: Qualitative data often reveals surprises that we miss in quantitative data. QDA offers us new ideas and perspectives, for insights we might otherwise miss.
• Building Effective Strategies: Insights from QDA are like strategic guides. They help businesses in crafting plans that match people’s desires.
• Creating Genuine Connections: Understanding people’s experiences lets businesses connect on a real level. This genuine connection helps build trust and loyalty, priceless for any business.

How to do Qualitative Data Analysis: 5 steps

Now we are going to show how you can do your own qualitative data analysis. We will guide you through this process step by step. As mentioned earlier, you will learn how to do qualitative data analysis manually , and also automatically using modern qualitative data and thematic analysis software.

To get best value from the analysis process and research process, it’s important to be super clear about the nature and scope of the question that’s being researched. This will help you select the research collection channels that are most likely to help you answer your question.

Depending on if you are a business looking to understand customer sentiment, or an academic surveying a school, your approach to qualitative data analysis will be unique.

Once you’re clear, there’s a sequence to follow. And, though there are differences in the manual and automatic approaches, the process steps are mostly the same.

The use case for our step-by-step guide is a company looking to collect data (customer feedback data), and analyze the customer feedback - in order to improve customer experience. By analyzing the customer feedback the company derives insights about their business and their customers. You can follow these same steps regardless of the nature of your research. Let’s get started.

Step 1: Gather your qualitative data and conduct research (Conduct qualitative research)

The first step of qualitative research is to do data collection. Put simply, data collection is gathering all of your data for analysis. A common situation is when qualitative data is spread across various sources.

Classic methods of gathering qualitative data

Most companies use traditional methods for gathering qualitative data: conducting interviews with research participants, running surveys, and running focus groups. This data is typically stored in documents, CRMs, databases and knowledge bases. It’s important to examine which data is available and needs to be included in your research project, based on its scope.

Using your existing qualitative feedback

As it becomes easier for customers to engage across a range of different channels, companies are gathering increasingly large amounts of both solicited and unsolicited qualitative feedback.

Most organizations have now invested in Voice of Customer programs , support ticketing systems, chatbot and support conversations, emails and even customer Slack chats.

These new channels provide companies with new ways of getting feedback, and also allow the collection of unstructured feedback data at scale.

The great thing about this data is that it contains a wealth of valubale insights and that it’s already there! When you have a new question about user behavior or your customers, you don’t need to create a new research study or set up a focus group. You can find most answers in the data you already have.

Typically, this data is stored in third-party solutions or a central database, but there are ways to export it or connect to a feedback analysis solution through integrations or an API.

Utilize untapped qualitative data channels

There are many online qualitative data sources you may not have considered. For example, you can find useful qualitative data in social media channels like Twitter or Facebook. Online forums, review sites, and online communities such as Discourse or Reddit also contain valuable data about your customers, or research questions.

If you are considering performing a qualitative benchmark analysis against competitors - the internet is your best friend, and review analysis is a great place to start. Gathering feedback in competitor reviews on sites like Trustpilot, G2, Capterra, Better Business Bureau or on app stores is a great way to perform a competitor benchmark analysis.

Customer feedback analysis software often has integrations into social media and review sites, or you could use a solution like DataMiner to scrape the reviews.

Step 2: Connect & organize all your qualitative data

Now you all have this qualitative data but there’s a problem, the data is unstructured. Before feedback can be analyzed and assigned any value, it needs to be organized in a single place. Why is this important? Consistency!

If all data is easily accessible in one place and analyzed in a consistent manner, you will have an easier time summarizing and making decisions based on this data.

The manual approach to organizing your data

The classic method of structuring qualitative data is to plot all the raw data you’ve gathered into a spreadsheet.

Typically, research and support teams would share large Excel sheets and different business units would make sense of the qualitative feedback data on their own. Each team collects and organizes the data in a way that best suits them, which means the feedback tends to be kept in separate silos.

An alternative and a more robust solution is to store feedback in a central database, like Snowflake or Amazon Redshift .

Keep in mind that when you organize your data in this way, you are often preparing it to be imported into another software. If you go the route of a database, you would need to use an API to push the feedback into a third-party software.

Computer-assisted qualitative data analysis software (CAQDAS)

Traditionally within the manual analysis approach (but not always), qualitative data is imported into CAQDAS software for coding.

In the early 2000s, CAQDAS software was popularised by developers such as ATLAS.ti, NVivo and MAXQDA and eagerly adopted by researchers to assist with the organizing and coding of data.  

The benefits of using computer-assisted qualitative data analysis software:

• Assists in the organizing of your data
• Opens you up to exploring different interpretations of your data analysis
• Allows you to share your dataset easier and allows group collaboration (allows for secondary analysis)

However you still need to code the data, uncover the themes and do the analysis yourself. Therefore it is still a manual approach.

Organizing your qualitative data in a feedback repository

Another solution to organizing your qualitative data is to upload it into a feedback repository where it can be unified with your other data , and easily searchable and taggable. There are a number of software solutions that act as a central repository for your qualitative research data. Here are a couple solutions that you could investigate:  

• Dovetail: Dovetail is a research repository with a focus on video and audio transcriptions. You can tag your transcriptions within the platform for theme analysis. You can also upload your other qualitative data such as research reports, survey responses, support conversations ( conversational analytics ), and customer interviews. Dovetail acts as a single, searchable repository. And makes it easier to collaborate with other people around your qualitative research.
• EnjoyHQ: EnjoyHQ is another research repository with similar functionality to Dovetail. It boasts a more sophisticated search engine, but it has a higher starting subscription cost.

Organizing your qualitative data in a feedback analytics platform

If you have a lot of qualitative customer or employee feedback, from the likes of customer surveys or employee surveys, you will benefit from a feedback analytics platform. A feedback analytics platform is a software that automates the process of both sentiment analysis and thematic analysis . Companies use the integrations offered by these platforms to directly tap into their qualitative data sources (review sites, social media, survey responses, etc.). The data collected is then organized and analyzed consistently within the platform.

If you have data prepared in a spreadsheet, it can also be imported into feedback analytics platforms.

Once all this rich data has been organized within the feedback analytics platform, it is ready to be coded and themed, within the same platform. Thematic is a feedback analytics platform that offers one of the largest libraries of integrations with qualitative data sources.

Step 3: Coding your qualitative data

Your feedback data is now organized in one place. Either within your spreadsheet, CAQDAS, feedback repository or within your feedback analytics platform. The next step is to code your feedback data so we can extract meaningful insights in the next step.

Coding is the process of labelling and organizing your data in such a way that you can then identify themes in the data, and the relationships between these themes.

To simplify the coding process, you will take small samples of your customer feedback data, come up with a set of codes, or categories capturing themes, and label each piece of feedback, systematically, for patterns and meaning. Then you will take a larger sample of data, revising and refining the codes for greater accuracy and consistency as you go.

If you choose to use a feedback analytics platform, much of this process will be automated and accomplished for you.

The terms to describe different categories of meaning (‘theme’, ‘code’, ‘tag’, ‘category’ etc) can be confusing as they are often used interchangeably.  For clarity, this article will use the term ‘code’.

To code means to identify key words or phrases and assign them to a category of meaning. “I really hate the customer service of this computer software company” would be coded as “poor customer service”.

How to manually code your qualitative data

• Decide whether you will use deductive or inductive coding. Deductive coding is when you create a list of predefined codes, and then assign them to the qualitative data. Inductive coding is the opposite of this, you create codes based on the data itself. Codes arise directly from the data and you label them as you go. You need to weigh up the pros and cons of each coding method and select the most appropriate.
• Read through the feedback data to get a broad sense of what it reveals. Now it’s time to start assigning your first set of codes to statements and sections of text.
• Keep repeating step 2, adding new codes and revising the code description as often as necessary.  Once it has all been coded, go through everything again, to be sure there are no inconsistencies and that nothing has been overlooked.
• Create a code frame to group your codes. The coding frame is the organizational structure of all your codes. And there are two commonly used types of coding frames, flat, or hierarchical. A hierarchical code frame will make it easier for you to derive insights from your analysis.
• Based on the number of times a particular code occurs, you can now see the common themes in your feedback data. This is insightful! If ‘bad customer service’ is a common code, it’s time to take action.

We have a detailed guide dedicated to manually coding your qualitative data .

Using software to speed up manual coding of qualitative data

An Excel spreadsheet is still a popular method for coding. But various software solutions can help speed up this process. Here are some examples.

• CAQDAS / NVivo - CAQDAS software has built-in functionality that allows you to code text within their software. You may find the interface the software offers easier for managing codes than a spreadsheet.
• Dovetail/EnjoyHQ - You can tag transcripts and other textual data within these solutions. As they are also repositories you may find it simpler to keep the coding in one platform.
• IBM SPSS - SPSS is a statistical analysis software that may make coding easier than in a spreadsheet.
• Ascribe - Ascribe’s ‘Coder’ is a coding management system. Its user interface will make it easier for you to manage your codes.

Automating the qualitative coding process using thematic analysis software

In solutions which speed up the manual coding process, you still have to come up with valid codes and often apply codes manually to pieces of feedback. But there are also solutions that automate both the discovery and the application of codes.

Advances in machine learning have now made it possible to read, code and structure qualitative data automatically. This type of automated coding is offered by thematic analysis software .

Automation makes it far simpler and faster to code the feedback and group it into themes. By incorporating natural language processing (NLP) into the software, the AI looks across sentences and phrases to identify common themes meaningful statements. Some automated solutions detect repeating patterns and assign codes to them, others make you train the AI by providing examples. You could say that the AI learns the meaning of the feedback on its own.

Thematic automates the coding of qualitative feedback regardless of source. There’s no need to set up themes or categories in advance. Simply upload your data and wait a few minutes. You can also manually edit the codes to further refine their accuracy.  Experiments conducted indicate that Thematic’s automated coding is just as accurate as manual coding .

Paired with sentiment analysis and advanced text analytics - these automated solutions become powerful for deriving quality business or research insights.

You could also build your own , if you have the resources!

The key benefits of using an automated coding solution

Automated analysis can often be set up fast and there’s the potential to uncover things that would never have been revealed if you had given the software a prescribed list of themes to look for.

Because the model applies a consistent rule to the data, it captures phrases or statements that a human eye might have missed.

Complete and consistent analysis of customer feedback enables more meaningful findings. Leading us into step 4.

Step 4: Analyze your data: Find meaningful insights

Now we are going to analyze our data to find insights. This is where we start to answer our research questions. Keep in mind that step 4 and step 5 (tell the story) have some overlap . This is because creating visualizations is both part of analysis process and reporting.

The task of uncovering insights is to scour through the codes that emerge from the data and draw meaningful correlations from them. It is also about making sure each insight is distinct and has enough data to support it.

Part of the analysis is to establish how much each code relates to different demographics and customer profiles, and identify whether there’s any relationship between these data points.

Manually create sub-codes to improve the quality of insights

If your code frame only has one level, you may find that your codes are too broad to be able to extract meaningful insights. This is where it is valuable to create sub-codes to your primary codes. This process is sometimes referred to as meta coding.

Note: If you take an inductive coding approach, you can create sub-codes as you are reading through your feedback data and coding it.

While time-consuming, this exercise will improve the quality of your analysis. Here is an example of what sub-codes could look like.

You need to carefully read your qualitative data to create quality sub-codes. But as you can see, the depth of analysis is greatly improved. By calculating the frequency of these sub-codes you can get insight into which  customer service problems you can immediately address.

Correlate the frequency of codes to customer segments

Many businesses use customer segmentation . And you may have your own respondent segments that you can apply to your qualitative analysis. Segmentation is the practise of dividing customers or research respondents into subgroups.

Segments can be based on:

• Demographic
• And any other data type that you care to segment by

It is particularly useful to see the occurrence of codes within your segments. If one of your customer segments is considered unimportant to your business, but they are the cause of nearly all customer service complaints, it may be in your best interest to focus attention elsewhere. This is a useful insight!

Manually visualizing coded qualitative data

There are formulas you can use to visualize key insights in your data. The formulas we will suggest are imperative if you are measuring a score alongside your feedback.

If you are collecting a metric alongside your qualitative data this is a key visualization. Impact answers the question: “What’s the impact of a code on my overall score?”. Using Net Promoter Score (NPS) as an example, first you need to:

• Calculate overall NPS
• Calculate NPS in the subset of responses that do not contain that theme
• Subtract B from A

Then you can use this simple formula to calculate code impact on NPS .

You can then visualize this data using a bar chart.

You can download our CX toolkit - it includes a template to recreate this.

Trends over time

This analysis can help you answer questions like: “Which codes are linked to decreases or increases in my score over time?”

We need to compare two sequences of numbers: NPS over time and code frequency over time . Using Excel, calculate the correlation between the two sequences, which can be either positive (the more codes the higher the NPS, see picture below), or negative (the more codes the lower the NPS).

Now you need to plot code frequency against the absolute value of code correlation with NPS. Here is the formula:

The visualization could look like this:

These are two examples, but there are more. For a third manual formula, and to learn why word clouds are not an insightful form of analysis, read our visualizations article .

Using a text analytics solution to automate analysis

Automated text analytics solutions enable codes and sub-codes to be pulled out of the data automatically. This makes it far faster and easier to identify what’s driving negative or positive results. And to pick up emerging trends and find all manner of rich insights in the data.

Another benefit of AI-driven text analytics software is its built-in capability for sentiment analysis, which provides the emotive context behind your feedback and other qualitative textual data therein.

Thematic provides text analytics that goes further by allowing users to apply their expertise on business context to edit or augment the AI-generated outputs.

Since the move away from manual research is generally about reducing the human element, adding human input to the technology might sound counter-intuitive. However, this is mostly to make sure important business nuances in the feedback aren’t missed during coding. The result is a higher accuracy of analysis. This is sometimes referred to as augmented intelligence .

Step 5: Report on your data: Tell the story

The last step of analyzing your qualitative data is to report on it, to tell the story. At this point, the codes are fully developed and the focus is on communicating the narrative to the audience.

A coherent outline of the qualitative research, the findings and the insights is vital for stakeholders to discuss and debate before they can devise a meaningful course of action.

Creating graphs and reporting in Powerpoint

Typically, qualitative researchers take the tried and tested approach of distilling their report into a series of charts, tables and other visuals which are woven into a narrative for presentation in Powerpoint.

Using visualization software for reporting

With data transformation and APIs, the analyzed data can be shared with data visualisation software, such as Power BI or Tableau , Google Studio or Looker. Power BI and Tableau are among the most preferred options.

Visualizing your insights inside a feedback analytics platform

Feedback analytics platforms, like Thematic, incorporate visualisation tools that intuitively turn key data and insights into graphs.  This removes the time consuming work of constructing charts to visually identify patterns and creates more time to focus on building a compelling narrative that highlights the insights, in bite-size chunks, for executive teams to review.

Using a feedback analytics platform with visualization tools means you don’t have to use a separate product for visualizations. You can export graphs into Powerpoints straight from the platforms.

Conclusion - Manual or Automated?

There are those who remain deeply invested in the manual approach - because it’s familiar, because they’re reluctant to spend money and time learning new software, or because they’ve been burned by the overpromises of AI.  

For projects that involve small datasets, manual analysis makes sense. For example, if the objective is simply to quantify a simple question like “Do customers prefer X concepts to Y?”. If the findings are being extracted from a small set of focus groups and interviews, sometimes it’s easier to just read them

However, as new generations come into the workplace, it’s technology-driven solutions that feel more comfortable and practical. And the merits are undeniable.  Especially if the objective is to go deeper and understand the ‘why’ behind customers’ preference for X or Y. And even more especially if time and money are considerations.

The ability to collect a free flow of qualitative feedback data at the same time as the metric means AI can cost-effectively scan, crunch, score and analyze a ton of feedback from one system in one go. And time-intensive processes like focus groups, or coding, that used to take weeks, can now be completed in a matter of hours or days.

But aside from the ever-present business case to speed things up and keep costs down, there are also powerful research imperatives for automated analysis of qualitative data: namely, accuracy and consistency.

Finding insights hidden in feedback requires consistency, especially in coding.  Not to mention catching all the ‘unknown unknowns’ that can skew research findings and steering clear of cognitive bias.

Some say without manual data analysis researchers won’t get an accurate “feel” for the insights. However, the larger data sets are, the harder it is to sort through the feedback and organize feedback that has been pulled from different places.  And, the more difficult it is to stay on course, the greater the risk of drawing incorrect, or incomplete, conclusions grows.

Though the process steps for qualitative data analysis have remained pretty much unchanged since psychologist Paul Felix Lazarsfeld paved the path a hundred years ago, the impact digital technology has had on types of qualitative feedback data and the approach to the analysis are profound.  

If you want to try an automated feedback analysis solution on your own qualitative data, you can get started with Thematic .

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What is data analysis? Examples and how to get started

Even with years of professional experience working with data, the term "data analysis" still sets off a panic button in my soul. And yes, when it comes to serious data analysis for your business, you'll eventually want data scientists on your side. But if you're just getting started, no panic attacks are required.

Table of contents:

What is data analysis?

Data analysis is the process of examining, filtering, adapting, and modeling data to help solve problems. Data analysis helps determine what is and isn't working, so you can make the changes needed to achieve your business goals. 

Keep in mind that data analysis includes analyzing both quantitative data (e.g., profits and sales) and qualitative data (e.g., surveys and case studies) to paint the whole picture. Here are two simple examples (of a nuanced topic) to show you what I mean.

An example of quantitative data analysis is an online jewelry store owner using inventory data to forecast and improve reordering accuracy. By collecting and analyzing inventory data on their SKUs, they're forecasting to improve reordering accuracy. The next time they order inventory, they order twice as many gold pieces as silver to meet customer demand.

An example of qualitative data analysis is a fitness studio owner collecting customer feedback to improve class offerings. The studio owner sends out an open-ended survey asking customers what types of exercises they enjoy the most. The owner then performs qualitative content analysis to identify the most frequently suggested exercises and incorporates these into future workout classes.

Why is data analysis important?

In case you need to convince your bosses, h ere's why it's worth implementing data analysis for your business.

Understand your target audience

You might think you know how to best target your audience, but are your assumptions backed by data? Data analysis can help answer questions like, "What demographics define my target audience?" or "What is my audience motivated by?"

Inform decisions

You don't need to toss and turn over a decision when the data points clearly to the answer. For instance, a restaurant could analyze which dishes on the menu are selling the most, helping them decide which ones to keep and which ones to change.

Improve budget accuracy

Data analysis can highlight areas in your business that are performing well and are worth investing more in, as well as areas that aren't generating enough revenue and should be cut. For example, a B2B software company might discover their product for enterprises is thriving while their small business solution lags behind. This discovery could prompt them to allocate more budget toward the enterprise product, resulting in better resource utilization.

Identify and solve problems

Let's say a cell phone manufacturer notices data showing a lot of customers returning a certain model. When they investigate, they find that model also happens to have the highest number of crashes. Once they identify and solve the technical issue, they can reduce the number of returns.

Types of data analysis (with examples)

There are five main types of data analysis—with increasingly scary-sounding names. Each one serves a different purpose, so take a look to see which makes the most sense for your situation. It's ok if you can't pronounce the one you choose. 

Text analysis: What is happening?

Here are a few methods used to perform text analysis, to give you a sense of how it's different from a human reading through the text: 

Word frequency identifies the most frequently used words. For example, a restaurant monitors social media mentions and measures the frequency of positive and negative keywords like "delicious" or "expensive" to determine how customers feel about their experience. 

Language detection indicates the language of text. For example, a global software company may use language detection on support tickets to connect customers with the appropriate agent. 

Keyword extraction automatically identifies the most used terms. For example, instead of sifting through thousands of reviews, a popular brand uses a keyword extractor to summarize the words or phrases that are most relevant. 

Statistical analysis: What happened?

Statistical analysis pulls past data to identify meaningful trends. Two primary categories of statistical analysis exist: descriptive and inferential.

Descriptive analysis

Here are a few methods used to perform descriptive analysis: 

Measures of frequency identify how frequently an event occurs. For example, a popular coffee chain sends out a survey asking customers what their favorite holiday drink is and uses measures of frequency to determine how often a particular drink is selected. 

Measures of central tendency use mean, median, and mode to identify results. For example, a dating app company might use measures of central tendency to determine the average age of its users.

Measures of dispersion measure how data is distributed across a range. For example, HR may use measures of dispersion to determine what salary to offer in a given field. 

Inferential analysis

Inferential analysis uses a sample of data to draw conclusions about a much larger population. This type of analysis is used when the population you're interested in analyzing is very large. 

Here are a few methods used when performing inferential analysis: 

Hypothesis testing identifies which variables impact a particular topic. For example, a business uses hypothesis testing to determine if increased sales were the result of a specific marketing campaign. 

Regression analysis shows the effect of independent variables on a dependent variable. For example, a rental car company may use regression analysis to determine the relationship between wait times and number of bad reviews. 

Diagnostic analysis: Why did it happen?

Diagnostic analysis, also referred to as root cause analysis, uncovers the causes of certain events or results. 

Here are a few methods used to perform diagnostic analysis: 

Time-series analysis analyzes data collected over a period of time. A retail store may use time-series analysis to determine that sales increase between October and December every year. 

Correlation analysis determines the strength of the relationship between variables. For example, a local ice cream shop may determine that as the temperature in the area rises, so do ice cream sales. 

Predictive analysis: What is likely to happen?

Predictive analysis aims to anticipate future developments and events. By analyzing past data, companies can predict future scenarios and make strategic decisions.  

Here are a few methods used to perform predictive analysis: 

Decision trees map out possible courses of action and outcomes. For example, a business may use a decision tree when deciding whether to downsize or expand. 

Prescriptive analysis: What action should we take?

The highest level of analysis, prescriptive analysis, aims to find the best action plan. Typically, AI tools model different outcomes to predict the best approach. While these tools serve to provide insight, they don't replace human consideration, so always use your human brain before going with the conclusion of your prescriptive analysis. Otherwise, your GPS might drive you into a lake.

Here are a few methods used to perform prescriptive analysis: 

Algorithms are used in technology to perform specific tasks. For example, banks use prescriptive algorithms to monitor customers' spending and recommend that they deactivate their credit card if fraud is suspected. 

Data analysis process: How to get started

The actual analysis is just one step in a much bigger process of using data to move your business forward. Here's a quick look at all the steps you need to take to make sure you're making informed decisions. 

Data decision

As with almost any project, the first step is to determine what problem you're trying to solve through data analysis. 

Make sure you get specific here. For example, a food delivery service may want to understand why customers are canceling their subscriptions. But to enable the most effective data analysis, they should pose a more targeted question, such as "How can we reduce customer churn without raising costs?" 

Data collection

Next, collect the required data from both internal and external sources. 

Internal data comes from within your business (think CRM software, internal reports, and archives), and helps you understand your business and processes.

External data originates from outside of the company (surveys, questionnaires, public data) and helps you understand your industry and your customers. 

Data cleaning

Data can be seriously misleading if it's not clean. So before you analyze, make sure you review the data you collected.  Depending on the type of data you have, cleanup will look different, but it might include: 

Removing unnecessary information 

Addressing structural errors like misspellings

Deleting duplicates

Trimming whitespace

Human checking for accuracy 

Data analysis

Now that you've compiled and cleaned the data, use one or more of the above types of data analysis to find relationships, patterns, and trends. 

Data analysis tools can speed up the data analysis process and remove the risk of inevitable human error. Here are some examples.

Spreadsheets sort, filter, analyze, and visualize data. 

Structured query language (SQL) tools manage and extract data in relational databases. 

Data interpretation

After you analyze the data, you'll need to go back to the original question you posed and draw conclusions from your findings. Here are some common pitfalls to avoid:

Correlation vs. causation: Just because two variables are associated doesn't mean they're necessarily related or dependent on one another. 

Confirmation bias: This occurs when you interpret data in a way that confirms your own preconceived notions. To avoid this, have multiple people interpret the data. 

Small sample size: If your sample size is too small or doesn't represent the demographics of your customers, you may get misleading results. If you run into this, consider widening your sample size to give you a more accurate representation. 

Data visualization

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Data analysis FAQ

Need a quick summary or still have a few nagging data analysis questions? I'm here for you.

What are the five types of data analysis?

The five types of data analysis are text analysis, statistical analysis, diagnostic analysis, predictive analysis, and prescriptive analysis.

Each type offers a unique lens for understanding data: text analysis provides insights into text-based content, statistical analysis focuses on numerical trends, diagnostic analysis looks into problem causes, predictive analysis deals with what may happen in the future, and prescriptive analysis gives actionable recommendations.

What is the data analysis process?

The data analysis process involves data decision, collection, cleaning, analysis, interpretation, and visualization. Every stage comes together to transform raw data into meaningful insights. Decision determines what data to collect, collection gathers the relevant information, cleaning ensures accuracy, analysis uncovers patterns, interpretation assigns meaning, and visualization presents the insights.

What is the main purpose of data analysis?

In business, the main purpose of data analysis is to uncover patterns, trends, and anomalies, and then use that information to make decisions, solve problems, and reach your business goals.

Related reading: 

This article was originally published in October 2022 and has since been updated with contributions from Cecilia Gillen and Dylan Reber. The most recent update was in September 2024.

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Shea is a content writer currently living in Charlotte, North Carolina. After graduating with a degree in Marketing from East Carolina University, she joined the digital marketing industry focusing on content and social media. In her free time, you can find Shea visiting her local farmers market, attending a country music concert, or planning her next adventure.

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Data Analysis in Research

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Data analysis in research involves systematically applying statistical and logical techniques to describe, illustrate, condense, and evaluate data. It is a crucial step that enables researchers to identify patterns, relationships, and trends within the data, transforming raw information into valuable insights. Through methods such as descriptive statistics, inferential statistics, and qualitative analysis, researchers can interpret their findings, draw conclusions, and support decision-making processes. An effective data analysis plan and robust methodology ensure the accuracy and reliability of research outcomes, ultimately contributing to the advancement of knowledge across various fields.

What is Data Analysis in Research?

Data analysis in research involves using statistical and logical techniques to describe, summarize, and compare collected data. This includes inspecting, cleaning, transforming, and modeling data to find useful information and support decision-making. Quantitative data provides measurable insights, and a solid research design ensures accuracy and reliability. This process helps validate hypotheses, identify patterns, and make informed conclusions, making it a crucial step in the scientific method.

Examples of Data analysis in Research

• Survey Analysis : Researchers collect survey responses from a sample population to gauge opinions, behaviors, or characteristics. Using descriptive statistics, they summarize the data through means, medians, and modes, and then inferential statistics to generalize findings to a larger population.
• Experimental Analysis : In scientific experiments, researchers manipulate one or more variables to observe the effect on a dependent variable. Data is analyzed using methods such as ANOVA or regression analysis to determine if changes in the independent variable(s) significantly affect the dependent variable.
• Content Analysis : Qualitative research often involves analyzing textual data, such as interview transcripts or open-ended survey responses. Researchers code the data to identify recurring themes, patterns, and categories, providing a deeper understanding of the subject matter.
• Correlation Studies : Researchers explore the relationship between two or more variables using correlation coefficients. For example, a study might examine the correlation between hours of study and academic performance to identify if there is a significant positive relationship.
• Longitudinal Analysis : This type of analysis involves collecting data from the same subjects over a period of time. Researchers analyze this data to observe changes and developments, such as studying the long-term effects of a specific educational intervention on student achievement.
• Meta-Analysis : By combining data from multiple studies, researchers perform a meta-analysis to increase the overall sample size and enhance the reliability of findings. This method helps in synthesizing research results to draw broader conclusions about a particular topic or intervention.

Data analysis in Qualitative Research

Data analysis in qualitative research involves systematically examining non-numeric data, such as interviews, observations, and textual materials, to identify patterns, themes, and meanings. Here are some key steps and methods used in qualitative data analysis:

• Coding : Researchers categorize the data by assigning labels or codes to specific segments of the text. These codes represent themes or concepts relevant to the research question.
• Thematic Analysis : This method involves identifying and analyzing patterns or themes within the data. Researchers review coded data to find recurring topics and construct a coherent narrative around these themes.
• Content Analysis : A systematic approach to categorize verbal or behavioral data to classify, summarize, and tabulate the data. This method often involves counting the frequency of specific words or phrases.
• Narrative Analysis : Researchers focus on the stories and experiences shared by participants, analyzing the structure, content, and context of the narratives to understand how individuals make sense of their experiences.
• Grounded Theory : This method involves generating a theory based on the data collected. Researchers collect and analyze data simultaneously, continually refining and adjusting their theoretical framework as new data emerges.
• Discourse Analysis : Examining language use and communication patterns within the data, researchers analyze how language constructs social realities and power relationships.
• Case Study Analysis : An in-depth analysis of a single case or multiple cases, exploring the complexities and unique aspects of each case to gain a deeper understanding of the phenomenon under study.

Data analysis in Quantitative Research

Data analysis in quantitative research involves the systematic application of statistical techniques to numerical data to identify patterns, relationships, and trends. Here are some common methods used in quantitative data analysis:

• Descriptive Statistics : This includes measures such as mean, median, mode, standard deviation, and range, which summarize and describe the main features of a data set.
• Inferential Statistics : Techniques like t-tests, chi-square tests, and ANOVA (Analysis of Variance) are used to make inferences or generalizations about a population based on a sample.
• Regression Analysis : This method examines the relationship between dependent and independent variables. Simple linear regression analyzes the relationship between two variables, while multiple regression examines the relationship between one dependent variable and several independent variables.
• Correlation Analysis : Researchers use correlation coefficients to measure the strength and direction of the relationship between two variables.
• Factor Analysis : This technique is used to identify underlying relationships between variables by grouping them into factors based on their correlations.
• Cluster Analysis : A method used to group a set of objects or cases into clusters, where objects in the same cluster are more similar to each other than to those in other clusters.
• Hypothesis Testing : This involves testing an assumption or hypothesis about a population parameter. Common tests include z-tests, t-tests, and chi-square tests, which help determine if there is enough evidence to reject the null hypothesis.
• Time Series Analysis : This method analyzes data points collected or recorded at specific time intervals to identify trends, cycles, and seasonal variations.
• Multivariate Analysis : Techniques like MANOVA (Multivariate Analysis of Variance) and PCA (Principal Component Analysis) are used to analyze data that involves multiple variables to understand their effect and relationships.
• Structural Equation Modeling (SEM) : A multivariate statistical analysis technique that is used to analyze structural relationships. This method is a combination of factor analysis and multiple regression analysis and is used to analyze the structural relationship between measured variables and latent constructs.

Data analysis in Research Methodology

Data analysis in research methodology involves the process of systematically applying statistical and logical techniques to describe, condense, recap, and evaluate data. Here are the key components and methods involved:

• Data Preparation : This step includes collecting, cleaning, and organizing raw data. Researchers ensure data quality by handling missing values, removing duplicates, and correcting errors.
• Descriptive Analysis : Researchers use descriptive statistics to summarize the basic features of the data. This includes measures such as mean, median, mode, standard deviation, and graphical representations like histograms and pie charts.
• Inferential Analysis : This involves using statistical tests to make inferences about the population from which the sample was drawn. Common techniques include t-tests, chi-square tests, ANOVA, and regression analysis.
• Qualitative Data Analysis : For non-numeric data, researchers employ methods like coding, thematic analysis, content analysis, narrative analysis, and discourse analysis to identify patterns and themes.
• Quantitative Data Analysis : For numeric data, researchers apply statistical methods such as correlation, regression, factor analysis, cluster analysis, and time series analysis to identify relationships and trends.
• Hypothesis Testing : Researchers test hypotheses using statistical methods to determine whether there is enough evidence to reject the null hypothesis. This involves calculating p-values and confidence intervals.
• Data Interpretation : This step involves interpreting the results of the data analysis. Researchers draw conclusions based on the statistical findings and relate them back to the research questions and objectives.
• Validation and Reliability : Ensuring the validity and reliability of the analysis is crucial. Researchers check for consistency in the results and use methods like cross-validation and reliability testing to confirm their findings.
• Visualization : Effective data visualization techniques, such as charts, graphs, and plots, are used to present the data in a clear and understandable manner, aiding in the interpretation and communication of results.
• Reporting : The final step involves reporting the results in a structured format, often including an introduction, methodology, results, discussion, and conclusion. This report should clearly convey the findings and their implications for the research question.

Types of Data analysis in Research

• Purpose : To summarize and describe the main features of a dataset.
• Methods : Mean, median, mode, standard deviation, frequency distributions, and graphical representations like histograms and pie charts.
• Example : Calculating the average test scores of students in a class.
• Purpose : To make inferences or generalizations about a population based on a sample.
• Methods : T-tests, chi-square tests, ANOVA (Analysis of Variance), regression analysis, and confidence intervals.
• Example : Testing whether a new teaching method significantly affects student performance compared to a traditional method.
• Purpose : To analyze data sets to find patterns, anomalies, and test hypotheses.
• Methods : Visualization techniques like box plots, scatter plots, and heat maps; summary statistics.
• Example : Visualizing the relationship between hours of study and exam scores using a scatter plot.
• Purpose : To make predictions about future outcomes based on historical data.
• Methods : Regression analysis, machine learning algorithms (e.g., decision trees, neural networks), and time series analysis.
• Example : Predicting student graduation rates based on their academic performance and demographic data.
• Purpose : To provide recommendations for decision-making based on data analysis.
• Methods : Optimization algorithms, simulation, and decision analysis.
• Example : Suggesting the best course of action for improving student retention rates based on various predictive factors.
• Purpose : To identify and understand cause-and-effect relationships.
• Methods : Controlled experiments, regression analysis, path analysis, and structural equation modeling (SEM).
• Example : Determining the impact of a specific intervention, like a new curriculum, on student learning outcomes.
• Purpose : To understand the specific mechanisms through which variables affect one another.
• Methods : Detailed modeling and simulation, often used in scientific research to understand biological or physical processes.
• Example : Studying how a specific drug interacts with biological pathways to affect patient health.

How to write Data analysis in Research

Data analysis is crucial for interpreting collected data and drawing meaningful conclusions. Follow these steps to write an effective data analysis section in your research.

1. Prepare Your Data

Ensure your data is clean and organized:

• Remove duplicates and irrelevant data.
• Check for errors and correct them.
• Categorize data if necessary.

2. Choose the Right Analysis Method

Select a method that fits your data type and research question:

• Quantitative Data : Use statistical analysis such as t-tests, ANOVA, regression analysis.
• Qualitative Data : Use thematic analysis, content analysis, or narrative analysis.

3. Describe Your Analytical Techniques

Clearly explain the methods you used:

• Software and Tools : Mention any software (e.g., SPSS, NVivo) used.
• Statistical Tests : Detail the statistical tests applied, such as chi-square tests or correlation analysis.
• Qualitative Techniques : Describe coding and theme identification processes.

4. Present Your Findings

Organize your findings logically:

• Use Tables and Figures : Display data in tables, graphs, and charts for clarity.
• Summarize Key Results : Highlight the most significant findings.
• Include Relevant Statistics : Report p-values, confidence intervals, means, and standard deviations.

5. Interpret the Results

Explain what your findings mean in the context of your research:

• Compare with Hypotheses : State whether the results support your hypotheses.
• Relate to Literature : Compare your results with previous studies.
• Discuss Implications : Explain the significance of your findings.

6. Discuss Limitations

Acknowledge any limitations in your data or analysis:

• Sample Size : Note if the sample size was small.
• Biases : Mention any potential biases in data collection.
• External Factors : Discuss any factors that might have influenced the results.

7. Conclude with a Summary

Wrap up your data analysis section:

• Restate Key Findings : Briefly summarize the main results.
• Future Research : Suggest areas for further investigation.

Importance of Data analysis in Research

Data analysis is a fundamental component of the research process. Here are five key points highlighting its importance:

• Enhances Accuracy and Reliability Data analysis ensures that research findings are accurate and reliable. By using statistical techniques, researchers can minimize errors and biases, ensuring that the results are dependable.
• Facilitates Informed Decision-Making Through data analysis, researchers can make informed decisions based on empirical evidence. This is crucial in fields like healthcare, business, and social sciences, where decisions impact policies, strategies, and outcomes.
• Identifies Trends and Patterns Analyzing data helps researchers uncover trends and patterns that might not be immediately visible. These insights can lead to new hypotheses and areas of study, advancing knowledge in the field.
• Supports Hypothesis Testing Data analysis is vital for testing hypotheses. Researchers can use statistical methods to determine whether their hypotheses are supported or refuted, which is essential for validating theories and advancing scientific understanding.
• Provides a Basis for Predictions By analyzing current and historical data, researchers can develop models that predict future outcomes. This predictive capability is valuable in numerous fields, including economics, climate science, and public health.

FAQ’s

What is the difference between qualitative and quantitative data analysis.

Qualitative analysis focuses on non-numerical data to understand concepts, while quantitative analysis deals with numerical data to identify patterns and relationships.

What is descriptive statistics?

Descriptive statistics summarize and describe the features of a data set, including measures like mean, median, mode, and standard deviation.

What is inferential statistics?

Inferential statistics use sample data to make generalizations about a larger population, often through hypothesis testing and confidence intervals.

What is regression analysis?

Regression analysis examines the relationship between dependent and independent variables, helping to predict outcomes and understand variable impacts.

What is the role of software in data analysis?

Software like SPSS, R, and Excel facilitate data analysis by providing tools for statistical calculations, visualization, and data management.

What are data visualization techniques?

Data visualization techniques include charts, graphs, and maps, which help in presenting data insights clearly and effectively.

What is data cleaning?

Data cleaning involves removing errors, inconsistencies, and missing values from a data set to ensure accuracy and reliability in analysis.

What is the significance of sample size in data analysis?

Sample size affects the accuracy and generalizability of results; larger samples generally provide more reliable insights.

How does correlation differ from causation?

Correlation indicates a relationship between variables, while causation implies one variable directly affects the other.

What are the ethical considerations in data analysis?

Ethical considerations include ensuring data privacy, obtaining informed consent, and avoiding data manipulation or misrepresentation.

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• How to Do Thematic Analysis | Step-by-Step Guide & Examples

How to Do Thematic Analysis | Step-by-Step Guide & Examples

Published on September 6, 2019 by Jack Caulfield . Revised on June 22, 2023.

Thematic analysis is a method of analyzing qualitative data . It is usually applied to a set of texts, such as an interview or transcripts . The researcher closely examines the data to identify common themes – topics, ideas and patterns of meaning that come up repeatedly.

There are various approaches to conducting thematic analysis, but the most common form follows a six-step process: familiarization, coding, generating themes, reviewing themes, defining and naming themes, and writing up. Following this process can also help you avoid confirmation bias when formulating your analysis.

This process was originally developed for psychology research by Virginia Braun and Victoria Clarke . However, thematic analysis is a flexible method that can be adapted to many different kinds of research.

Table of contents

When to use thematic analysis, different approaches to thematic analysis, step 1: familiarization, step 2: coding, step 3: generating themes, step 4: reviewing themes, step 5: defining and naming themes, step 6: writing up, other interesting articles.

Thematic analysis is a good approach to research where you’re trying to find out something about people’s views, opinions, knowledge, experiences or values from a set of qualitative data – for example, interview transcripts , social media profiles, or survey responses .

Some types of research questions you might use thematic analysis to answer:

• How do patients perceive doctors in a hospital setting?
• What are young women’s experiences on dating sites?
• What are non-experts’ ideas and opinions about climate change?
• How is gender constructed in high school history teaching?

To answer any of these questions, you would collect data from a group of relevant participants and then analyze it. Thematic analysis allows you a lot of flexibility in interpreting the data, and allows you to approach large data sets more easily by sorting them into broad themes.

However, it also involves the risk of missing nuances in the data. Thematic analysis is often quite subjective and relies on the researcher’s judgement, so you have to reflect carefully on your own choices and interpretations.

Pay close attention to the data to ensure that you’re not picking up on things that are not there – or obscuring things that are.

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Once you’ve decided to use thematic analysis, there are different approaches to consider.

There’s the distinction between inductive and deductive approaches:

• An inductive approach involves allowing the data to determine your themes.
• A deductive approach involves coming to the data with some preconceived themes you expect to find reflected there, based on theory or existing knowledge.

Ask yourself: Does my theoretical framework give me a strong idea of what kind of themes I expect to find in the data (deductive), or am I planning to develop my own framework based on what I find (inductive)?

There’s also the distinction between a semantic and a latent approach:

• A semantic approach involves analyzing the explicit content of the data.
• A latent approach involves reading into the subtext and assumptions underlying the data.

Ask yourself: Am I interested in people’s stated opinions (semantic) or in what their statements reveal about their assumptions and social context (latent)?

After you’ve decided thematic analysis is the right method for analyzing your data, and you’ve thought about the approach you’re going to take, you can follow the six steps developed by Braun and Clarke .

The first step is to get to know our data. It’s important to get a thorough overview of all the data we collected before we start analyzing individual items.

This might involve transcribing audio , reading through the text and taking initial notes, and generally looking through the data to get familiar with it.

Next up, we need to code the data. Coding means highlighting sections of our text – usually phrases or sentences – and coming up with shorthand labels or “codes” to describe their content.

Let’s take a short example text. Say we’re researching perceptions of climate change among conservative voters aged 50 and up, and we have collected data through a series of interviews. An extract from one interview looks like this:

In this extract, we’ve highlighted various phrases in different colors corresponding to different codes. Each code describes the idea or feeling expressed in that part of the text.

At this stage, we want to be thorough: we go through the transcript of every interview and highlight everything that jumps out as relevant or potentially interesting. As well as highlighting all the phrases and sentences that match these codes, we can keep adding new codes as we go through the text.

After we’ve been through the text, we collate together all the data into groups identified by code. These codes allow us to gain a a condensed overview of the main points and common meanings that recur throughout the data.

Next, we look over the codes we’ve created, identify patterns among them, and start coming up with themes.

Themes are generally broader than codes. Most of the time, you’ll combine several codes into a single theme. In our example, we might start combining codes into themes like this:

At this stage, we might decide that some of our codes are too vague or not relevant enough (for example, because they don’t appear very often in the data), so they can be discarded.

Other codes might become themes in their own right. In our example, we decided that the code “uncertainty” made sense as a theme, with some other codes incorporated into it.

Again, what we decide will vary according to what we’re trying to find out. We want to create potential themes that tell us something helpful about the data for our purposes.

Now we have to make sure that our themes are useful and accurate representations of the data. Here, we return to the data set and compare our themes against it. Are we missing anything? Are these themes really present in the data? What can we change to make our themes work better?

If we encounter problems with our themes, we might split them up, combine them, discard them or create new ones: whatever makes them more useful and accurate.

For example, we might decide upon looking through the data that “changing terminology” fits better under the “uncertainty” theme than under “distrust of experts,” since the data labelled with this code involves confusion, not necessarily distrust.

Now that you have a final list of themes, it’s time to name and define each of them.

Defining themes involves formulating exactly what we mean by each theme and figuring out how it helps us understand the data.

Naming themes involves coming up with a succinct and easily understandable name for each theme.

For example, we might look at “distrust of experts” and determine exactly who we mean by “experts” in this theme. We might decide that a better name for the theme is “distrust of authority” or “conspiracy thinking”.

Finally, we’ll write up our analysis of the data. Like all academic texts, writing up a thematic analysis requires an introduction to establish our research question, aims and approach.

We should also include a methodology section, describing how we collected the data (e.g. through semi-structured interviews or open-ended survey questions ) and explaining how we conducted the thematic analysis itself.

The results or findings section usually addresses each theme in turn. We describe how often the themes come up and what they mean, including examples from the data as evidence. Finally, our conclusion explains the main takeaways and shows how the analysis has answered our research question.

In our example, we might argue that conspiracy thinking about climate change is widespread among older conservative voters, point out the uncertainty with which many voters view the issue, and discuss the role of misinformation in respondents’ perceptions.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Measures of central tendency
• Chi square tests
• Confidence interval
• Quartiles & Quantiles
• Cluster sampling
• Stratified sampling
• Discourse analysis
• Cohort study
• Peer review
• Ethnography

Research bias

• Implicit bias
• Cognitive bias
• Conformity bias
• Hawthorne effect
• Availability heuristic
• Attrition bias
• Social desirability bias

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Data Analysis

• Introduction to Data Analysis
• Quantitative Analysis Tools
• Qualitative Analysis Tools
• Mixed Methods Analysis
• Geospatial Analysis
• Further Reading

What is Data Analysis?

According to the federal government, data analysis is "the process of systematically applying statistical and/or logical techniques to describe and illustrate, condense and recap, and evaluate data" ( Responsible Conduct in Data Management ). Important components of data analysis include searching for patterns, remaining unbiased in drawing inference from data, practicing responsible  data management , and maintaining "honest and accurate analysis" ( Responsible Conduct in Data Management ). 

In order to understand data analysis further, it can be helpful to take a step back and understand the question "What is data?". Many of us associate data with spreadsheets of numbers and values, however, data can encompass much more than that. According to the federal government, data is "The recorded factual material commonly accepted in the scientific community as necessary to validate research findings" ( OMB Circular 110 ). This broad definition can include information in many formats. 

Some examples of types of data are as follows:

• Photographs 
• Hand-written notes from field observation
• Machine learning training data sets
• Ethnographic interview transcripts
• Sheet music
• Scripts for plays and musicals 
• Observations from laboratory experiments ( CMU Data 101 )

Thus, data analysis includes the processing and manipulation of these data sources in order to gain additional insight from data, answer a research question, or confirm a research hypothesis. 

Data analysis falls within the larger research data lifecycle, as seen below. 

( University of Virginia )

Why Analyze Data?

Through data analysis, a researcher can gain additional insight from data and draw conclusions to address the research question or hypothesis. Use of data analysis tools helps researchers understand and interpret data. 

What are the Types of Data Analysis?

Data analysis can be quantitative, qualitative, or mixed methods. 

Quantitative research typically involves numbers and "close-ended questions and responses" ( Creswell & Creswell, 2018 , p. 3). Quantitative research tests variables against objective theories, usually measured and collected on instruments and analyzed using statistical procedures ( Creswell & Creswell, 2018 , p. 4). Quantitative analysis usually uses deductive reasoning. 

Qualitative  research typically involves words and "open-ended questions and responses" ( Creswell & Creswell, 2018 , p. 3). According to Creswell & Creswell, "qualitative research is an approach for exploring and understanding the meaning individuals or groups ascribe to a social or human problem" ( 2018 , p. 4). Thus, qualitative analysis usually invokes inductive reasoning. 

Mixed methods  research uses methods from both quantitative and qualitative research approaches. Mixed methods research works under the "core assumption... that the integration of qualitative and quantitative data yields additional insight beyond the information provided by either the quantitative or qualitative data alone" ( Creswell & Creswell, 2018 , p. 4). 

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• Indian J Anaesth
• v.60(9); 2016 Sep

Basic statistical tools in research and data analysis

Zulfiqar ali.

Department of Anaesthesiology, Division of Neuroanaesthesiology, Sheri Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu and Kashmir, India

S Bala Bhaskar

1 Department of Anaesthesiology and Critical Care, Vijayanagar Institute of Medical Sciences, Bellary, Karnataka, India

Statistical methods involved in carrying out a study include planning, designing, collecting data, analysing, drawing meaningful interpretation and reporting of the research findings. The statistical analysis gives meaning to the meaningless numbers, thereby breathing life into a lifeless data. The results and inferences are precise only if proper statistical tests are used. This article will try to acquaint the reader with the basic research tools that are utilised while conducting various studies. The article covers a brief outline of the variables, an understanding of quantitative and qualitative variables and the measures of central tendency. An idea of the sample size estimation, power analysis and the statistical errors is given. Finally, there is a summary of parametric and non-parametric tests used for data analysis.

INTRODUCTION

Statistics is a branch of science that deals with the collection, organisation, analysis of data and drawing of inferences from the samples to the whole population.[ 1 ] This requires a proper design of the study, an appropriate selection of the study sample and choice of a suitable statistical test. An adequate knowledge of statistics is necessary for proper designing of an epidemiological study or a clinical trial. Improper statistical methods may result in erroneous conclusions which may lead to unethical practice.[ 2 ]

Variable is a characteristic that varies from one individual member of population to another individual.[ 3 ] Variables such as height and weight are measured by some type of scale, convey quantitative information and are called as quantitative variables. Sex and eye colour give qualitative information and are called as qualitative variables[ 3 ] [ Figure 1 ].

Classification of variables

Quantitative variables

Quantitative or numerical data are subdivided into discrete and continuous measurements. Discrete numerical data are recorded as a whole number such as 0, 1, 2, 3,… (integer), whereas continuous data can assume any value. Observations that can be counted constitute the discrete data and observations that can be measured constitute the continuous data. Examples of discrete data are number of episodes of respiratory arrests or the number of re-intubations in an intensive care unit. Similarly, examples of continuous data are the serial serum glucose levels, partial pressure of oxygen in arterial blood and the oesophageal temperature.

A hierarchical scale of increasing precision can be used for observing and recording the data which is based on categorical, ordinal, interval and ratio scales [ Figure 1 ].

Categorical or nominal variables are unordered. The data are merely classified into categories and cannot be arranged in any particular order. If only two categories exist (as in gender male and female), it is called as a dichotomous (or binary) data. The various causes of re-intubation in an intensive care unit due to upper airway obstruction, impaired clearance of secretions, hypoxemia, hypercapnia, pulmonary oedema and neurological impairment are examples of categorical variables.

Ordinal variables have a clear ordering between the variables. However, the ordered data may not have equal intervals. Examples are the American Society of Anesthesiologists status or Richmond agitation-sedation scale.

Interval variables are similar to an ordinal variable, except that the intervals between the values of the interval variable are equally spaced. A good example of an interval scale is the Fahrenheit degree scale used to measure temperature. With the Fahrenheit scale, the difference between 70° and 75° is equal to the difference between 80° and 85°: The units of measurement are equal throughout the full range of the scale.

Ratio scales are similar to interval scales, in that equal differences between scale values have equal quantitative meaning. However, ratio scales also have a true zero point, which gives them an additional property. For example, the system of centimetres is an example of a ratio scale. There is a true zero point and the value of 0 cm means a complete absence of length. The thyromental distance of 6 cm in an adult may be twice that of a child in whom it may be 3 cm.

STATISTICS: DESCRIPTIVE AND INFERENTIAL STATISTICS

Descriptive statistics[ 4 ] try to describe the relationship between variables in a sample or population. Descriptive statistics provide a summary of data in the form of mean, median and mode. Inferential statistics[ 4 ] use a random sample of data taken from a population to describe and make inferences about the whole population. It is valuable when it is not possible to examine each member of an entire population. The examples if descriptive and inferential statistics are illustrated in Table 1 .

Example of descriptive and inferential statistics

Descriptive statistics

The extent to which the observations cluster around a central location is described by the central tendency and the spread towards the extremes is described by the degree of dispersion.

Measures of central tendency

The measures of central tendency are mean, median and mode.[ 6 ] Mean (or the arithmetic average) is the sum of all the scores divided by the number of scores. Mean may be influenced profoundly by the extreme variables. For example, the average stay of organophosphorus poisoning patients in ICU may be influenced by a single patient who stays in ICU for around 5 months because of septicaemia. The extreme values are called outliers. The formula for the mean is

where x = each observation and n = number of observations. Median[ 6 ] is defined as the middle of a distribution in a ranked data (with half of the variables in the sample above and half below the median value) while mode is the most frequently occurring variable in a distribution. Range defines the spread, or variability, of a sample.[ 7 ] It is described by the minimum and maximum values of the variables. If we rank the data and after ranking, group the observations into percentiles, we can get better information of the pattern of spread of the variables. In percentiles, we rank the observations into 100 equal parts. We can then describe 25%, 50%, 75% or any other percentile amount. The median is the 50 th percentile. The interquartile range will be the observations in the middle 50% of the observations about the median (25 th -75 th percentile). Variance[ 7 ] is a measure of how spread out is the distribution. It gives an indication of how close an individual observation clusters about the mean value. The variance of a population is defined by the following formula:

where σ 2 is the population variance, X is the population mean, X i is the i th element from the population and N is the number of elements in the population. The variance of a sample is defined by slightly different formula:

where s 2 is the sample variance, x is the sample mean, x i is the i th element from the sample and n is the number of elements in the sample. The formula for the variance of a population has the value ‘ n ’ as the denominator. The expression ‘ n −1’ is known as the degrees of freedom and is one less than the number of parameters. Each observation is free to vary, except the last one which must be a defined value. The variance is measured in squared units. To make the interpretation of the data simple and to retain the basic unit of observation, the square root of variance is used. The square root of the variance is the standard deviation (SD).[ 8 ] The SD of a population is defined by the following formula:

where σ is the population SD, X is the population mean, X i is the i th element from the population and N is the number of elements in the population. The SD of a sample is defined by slightly different formula:

where s is the sample SD, x is the sample mean, x i is the i th element from the sample and n is the number of elements in the sample. An example for calculation of variation and SD is illustrated in Table 2 .

Example of mean, variance, standard deviation

Normal distribution or Gaussian distribution

Most of the biological variables usually cluster around a central value, with symmetrical positive and negative deviations about this point.[ 1 ] The standard normal distribution curve is a symmetrical bell-shaped. In a normal distribution curve, about 68% of the scores are within 1 SD of the mean. Around 95% of the scores are within 2 SDs of the mean and 99% within 3 SDs of the mean [ Figure 2 ].

Normal distribution curve

Skewed distribution

It is a distribution with an asymmetry of the variables about its mean. In a negatively skewed distribution [ Figure 3 ], the mass of the distribution is concentrated on the right of Figure 1 . In a positively skewed distribution [ Figure 3 ], the mass of the distribution is concentrated on the left of the figure leading to a longer right tail.

Curves showing negatively skewed and positively skewed distribution

Inferential statistics

In inferential statistics, data are analysed from a sample to make inferences in the larger collection of the population. The purpose is to answer or test the hypotheses. A hypothesis (plural hypotheses) is a proposed explanation for a phenomenon. Hypothesis tests are thus procedures for making rational decisions about the reality of observed effects.

Probability is the measure of the likelihood that an event will occur. Probability is quantified as a number between 0 and 1 (where 0 indicates impossibility and 1 indicates certainty).

In inferential statistics, the term ‘null hypothesis’ ( H 0 ‘ H-naught ,’ ‘ H-null ’) denotes that there is no relationship (difference) between the population variables in question.[ 9 ]

Alternative hypothesis ( H 1 and H a ) denotes that a statement between the variables is expected to be true.[ 9 ]

The P value (or the calculated probability) is the probability of the event occurring by chance if the null hypothesis is true. The P value is a numerical between 0 and 1 and is interpreted by researchers in deciding whether to reject or retain the null hypothesis [ Table 3 ].

P values with interpretation

If P value is less than the arbitrarily chosen value (known as α or the significance level), the null hypothesis (H0) is rejected [ Table 4 ]. However, if null hypotheses (H0) is incorrectly rejected, this is known as a Type I error.[ 11 ] Further details regarding alpha error, beta error and sample size calculation and factors influencing them are dealt with in another section of this issue by Das S et al .[ 12 ]

Illustration for null hypothesis

PARAMETRIC AND NON-PARAMETRIC TESTS

Numerical data (quantitative variables) that are normally distributed are analysed with parametric tests.[ 13 ]

Two most basic prerequisites for parametric statistical analysis are:

• The assumption of normality which specifies that the means of the sample group are normally distributed
• The assumption of equal variance which specifies that the variances of the samples and of their corresponding population are equal.

However, if the distribution of the sample is skewed towards one side or the distribution is unknown due to the small sample size, non-parametric[ 14 ] statistical techniques are used. Non-parametric tests are used to analyse ordinal and categorical data.

Parametric tests

The parametric tests assume that the data are on a quantitative (numerical) scale, with a normal distribution of the underlying population. The samples have the same variance (homogeneity of variances). The samples are randomly drawn from the population, and the observations within a group are independent of each other. The commonly used parametric tests are the Student's t -test, analysis of variance (ANOVA) and repeated measures ANOVA.

Student's t -test

Student's t -test is used to test the null hypothesis that there is no difference between the means of the two groups. It is used in three circumstances:

where X = sample mean, u = population mean and SE = standard error of mean

where X 1 − X 2 is the difference between the means of the two groups and SE denotes the standard error of the difference.

• To test if the population means estimated by two dependent samples differ significantly (the paired t -test). A usual setting for paired t -test is when measurements are made on the same subjects before and after a treatment.

The formula for paired t -test is:

where d is the mean difference and SE denotes the standard error of this difference.

The group variances can be compared using the F -test. The F -test is the ratio of variances (var l/var 2). If F differs significantly from 1.0, then it is concluded that the group variances differ significantly.

Analysis of variance

The Student's t -test cannot be used for comparison of three or more groups. The purpose of ANOVA is to test if there is any significant difference between the means of two or more groups.

In ANOVA, we study two variances – (a) between-group variability and (b) within-group variability. The within-group variability (error variance) is the variation that cannot be accounted for in the study design. It is based on random differences present in our samples.

However, the between-group (or effect variance) is the result of our treatment. These two estimates of variances are compared using the F-test.

A simplified formula for the F statistic is:

where MS b is the mean squares between the groups and MS w is the mean squares within groups.

Repeated measures analysis of variance

As with ANOVA, repeated measures ANOVA analyses the equality of means of three or more groups. However, a repeated measure ANOVA is used when all variables of a sample are measured under different conditions or at different points in time.

As the variables are measured from a sample at different points of time, the measurement of the dependent variable is repeated. Using a standard ANOVA in this case is not appropriate because it fails to model the correlation between the repeated measures: The data violate the ANOVA assumption of independence. Hence, in the measurement of repeated dependent variables, repeated measures ANOVA should be used.

Non-parametric tests

When the assumptions of normality are not met, and the sample means are not normally, distributed parametric tests can lead to erroneous results. Non-parametric tests (distribution-free test) are used in such situation as they do not require the normality assumption.[ 15 ] Non-parametric tests may fail to detect a significant difference when compared with a parametric test. That is, they usually have less power.

As is done for the parametric tests, the test statistic is compared with known values for the sampling distribution of that statistic and the null hypothesis is accepted or rejected. The types of non-parametric analysis techniques and the corresponding parametric analysis techniques are delineated in Table 5 .

Analogue of parametric and non-parametric tests

Median test for one sample: The sign test and Wilcoxon's signed rank test

The sign test and Wilcoxon's signed rank test are used for median tests of one sample. These tests examine whether one instance of sample data is greater or smaller than the median reference value.

This test examines the hypothesis about the median θ0 of a population. It tests the null hypothesis H0 = θ0. When the observed value (Xi) is greater than the reference value (θ0), it is marked as+. If the observed value is smaller than the reference value, it is marked as − sign. If the observed value is equal to the reference value (θ0), it is eliminated from the sample.

If the null hypothesis is true, there will be an equal number of + signs and − signs.

The sign test ignores the actual values of the data and only uses + or − signs. Therefore, it is useful when it is difficult to measure the values.

Wilcoxon's signed rank test

There is a major limitation of sign test as we lose the quantitative information of the given data and merely use the + or – signs. Wilcoxon's signed rank test not only examines the observed values in comparison with θ0 but also takes into consideration the relative sizes, adding more statistical power to the test. As in the sign test, if there is an observed value that is equal to the reference value θ0, this observed value is eliminated from the sample.

Wilcoxon's rank sum test ranks all data points in order, calculates the rank sum of each sample and compares the difference in the rank sums.

Mann-Whitney test

It is used to test the null hypothesis that two samples have the same median or, alternatively, whether observations in one sample tend to be larger than observations in the other.

Mann–Whitney test compares all data (xi) belonging to the X group and all data (yi) belonging to the Y group and calculates the probability of xi being greater than yi: P (xi > yi). The null hypothesis states that P (xi > yi) = P (xi < yi) =1/2 while the alternative hypothesis states that P (xi > yi) ≠1/2.

Kolmogorov-Smirnov test

The two-sample Kolmogorov-Smirnov (KS) test was designed as a generic method to test whether two random samples are drawn from the same distribution. The null hypothesis of the KS test is that both distributions are identical. The statistic of the KS test is a distance between the two empirical distributions, computed as the maximum absolute difference between their cumulative curves.

Kruskal-Wallis test

The Kruskal–Wallis test is a non-parametric test to analyse the variance.[ 14 ] It analyses if there is any difference in the median values of three or more independent samples. The data values are ranked in an increasing order, and the rank sums calculated followed by calculation of the test statistic.

Jonckheere test

In contrast to Kruskal–Wallis test, in Jonckheere test, there is an a priori ordering that gives it a more statistical power than the Kruskal–Wallis test.[ 14 ]

Friedman test

The Friedman test is a non-parametric test for testing the difference between several related samples. The Friedman test is an alternative for repeated measures ANOVAs which is used when the same parameter has been measured under different conditions on the same subjects.[ 13 ]

Tests to analyse the categorical data

Chi-square test, Fischer's exact test and McNemar's test are used to analyse the categorical or nominal variables. The Chi-square test compares the frequencies and tests whether the observed data differ significantly from that of the expected data if there were no differences between groups (i.e., the null hypothesis). It is calculated by the sum of the squared difference between observed ( O ) and the expected ( E ) data (or the deviation, d ) divided by the expected data by the following formula:

A Yates correction factor is used when the sample size is small. Fischer's exact test is used to determine if there are non-random associations between two categorical variables. It does not assume random sampling, and instead of referring a calculated statistic to a sampling distribution, it calculates an exact probability. McNemar's test is used for paired nominal data. It is applied to 2 × 2 table with paired-dependent samples. It is used to determine whether the row and column frequencies are equal (that is, whether there is ‘marginal homogeneity’). The null hypothesis is that the paired proportions are equal. The Mantel-Haenszel Chi-square test is a multivariate test as it analyses multiple grouping variables. It stratifies according to the nominated confounding variables and identifies any that affects the primary outcome variable. If the outcome variable is dichotomous, then logistic regression is used.

SOFTWARES AVAILABLE FOR STATISTICS, SAMPLE SIZE CALCULATION AND POWER ANALYSIS

Numerous statistical software systems are available currently. The commonly used software systems are Statistical Package for the Social Sciences (SPSS – manufactured by IBM corporation), Statistical Analysis System ((SAS – developed by SAS Institute North Carolina, United States of America), R (designed by Ross Ihaka and Robert Gentleman from R core team), Minitab (developed by Minitab Inc), Stata (developed by StataCorp) and the MS Excel (developed by Microsoft).

There are a number of web resources which are related to statistical power analyses. A few are:

• StatPages.net – provides links to a number of online power calculators
• G-Power – provides a downloadable power analysis program that runs under DOS
• Power analysis for ANOVA designs an interactive site that calculates power or sample size needed to attain a given power for one effect in a factorial ANOVA design
• SPSS makes a program called SamplePower. It gives an output of a complete report on the computer screen which can be cut and paste into another document.

It is important that a researcher knows the concepts of the basic statistical methods used for conduct of a research study. This will help to conduct an appropriately well-designed study leading to valid and reliable results. Inappropriate use of statistical techniques may lead to faulty conclusions, inducing errors and undermining the significance of the article. Bad statistics may lead to bad research, and bad research may lead to unethical practice. Hence, an adequate knowledge of statistics and the appropriate use of statistical tests are important. An appropriate knowledge about the basic statistical methods will go a long way in improving the research designs and producing quality medical research which can be utilised for formulating the evidence-based guidelines.

Financial support and sponsorship

Conflicts of interest.

There are no conflicts of interest.

• Open access
• Published: 09 September 2024

The causal relationship between CSF metabolites and GBM: a two-sample mendelian randomization analysis

• Haijun Bao 1 , 2 ,
• Yiyang Chen 1 ,
• Zijun Meng 1 &
• Zheng Chu 1 , 2  

BMC Cancer volume  24 , Article number:  1119 ( 2024 ) Cite this article

Metrics details

Glioblastoma multiforme (GBM) is a highly aggressive primary malignant brain tumor characterized by rapid progression, poor prognosis, and high mortality rates. Understanding the relationship between cerebrospinal fluid (CSF) metabolites and GBM is crucial for identifying potential biomarkers and pathways involved in the pathogenesis of this devastating disease.

In this study, Mendelian randomization (MR) analysis was employed to investigate the causal relationship between 338 CSF metabolites and GBM. The data for metabolites were obtained from a genome-wide association study summary dataset based on 291 individuals, and the GBM data was derived from FinnGen included 91 cases and 174,006 controls of European descent. The Inverse Variance Weighted method was utilized to estimate the causal effects. Supplementary comprehensive assessments of causal effects between CSF metabolites and GBM were conducted using MR-Egger regression, Weighted Median, Simple Mode, and Weighted Mode methods. Additionally, tests for heterogeneity and pleiotropy were performed.

Through MR analysis, a total of 12 identified metabolites and 2 with unknown chemical properties were found to have a causal relationship with GBM. 1-palmitoyl-2-stearoyl-gpc (16:0/18:0), 7-alpha-hydroxy-3-oxo-4-cholestenoate, Alpha-tocopherol, Behenoyl sphingomyelin (d18:1/22:0), Cysteinylglycine, Maleate, Uracil, Valine, X-12,101, X-12,104 and Butyrate (4:0) are associated with an increased risk of GBM. N1-methylinosine, Stachydrine and Succinylcarnitine (c4-dc) are associated with decreased GBM risk.

In conclusion, this study sheds light on the intricate interplay between CSF metabolites and GBM, offering novel perspectives on disease mechanisms and potential treatment avenues. By elucidating the role of CSF metabolites in GBM pathogenesis, this research contributes to the advancement of diagnostic capabilities and targeted therapeutic interventions for this aggressive brain tumor. Further exploration of these findings may lead to improved management strategies and better outcomes for patients with GBM.

Peer Review reports

Introduction

Glioblastoma multiforme (GBM) is one of the most aggressive primary malignant brain tumors, characterized by rapid progression, poor prognosis, and high mortality rates [ 1 ]. According to the 2016 WHO classification, gliomas are divided into four grades: low-grade gliomas (LGG grades I and II) and high-grade gliomas (HGG grades III and IV) [ 2 ]. GBM, the most malignant subtype, accounts for over 50% of all gliomas and more than 15% of primary brain tumors [ 3 ]. Due to its immunosuppressive microenvironment and tendency for recurrence, GBM stands out as one of the most challenging tumors, imposing a significant societal burden.

Cerebrospinal fluid (CSF) envelops the cerebral and spinal regions within the meningeal cavities, playing a crucial role in maintaining brain homeostasis, providing nutrients, and clearing waste. Thanks to the presence of the blood-brain barrier, CSF maintains its distinctive environment, safeguarding the normal functioning of neurons. Due to their direct exposure to GBM, CSF metabolites hold the potential to mirror metabolic alterations linked to tumor initiation, presence, and progression [ 4 ]. GBM, as a highly invasive brain tumor, can disrupt the normal flow and composition of CSF, leading to increased intracranial pressure and hydrocephalus. Additionally, GBM cells can infiltrate the soft meninges and spread along the CSF pathways [ 5 ]. Therefore, CSF analysis, including cytology and molecular spectrum analysis, is pivotal for diagnosing and monitoring GBM, as it may contain tumor cells and biomarkers reflecting the disease state. Understanding the dynamic interplay between GBM and CSF is crucial for enhancing diagnostic and therapeutic strategies against this devastating disease.

Studying CSF presents both challenges and valuable opportunities in understanding various neurological disorders. One of the primary hurdles lies in obtaining CSF samples, typically acquired through invasive procedures like lumbar punctures, posing risks and discomfort to patients. Consequently, most human metabolomics research has focused on more accessible sample types such as blood or urine. Furthermore, CSF composition is dynamic, influenced by factors like age, gender, and disease status, making standardization and interpretation complex [ 6 ]. Despite these challenges, researching CSF holds immense value. CSF serves as a direct window into the central nervous system, reflecting biochemical, cellular, and molecular changes associated with neurological disorders like Alzheimer’s disease, multiple sclerosis, and brain tumors [ 7 , 8 , 9 ]. Analysis of CSF biomarkers provides diagnostic, prognostic, and therapeutic insights, aiding in disease detection, monitoring, and treatment response assessment. Rogachev et al. found that there were partial differences in the correlation of plasma and CSF metabolites between HGG and healthy control groups [ 4 ]. Ji et al. demonstrated that CSF metabolites could be used to predict glioma grade and leptomeningeal metastasis [ 10 ]. So, by overcoming technical barriers and leveraging the rich information provided by CSF, researchers can significantly enhance our understanding and management of neurological diseases, ultimately improving patient outcomes.

In this study, we employed Mendelian randomization (MR) analysis to investigate the causal relationship between CSF metabolites and GBM. MR is a robust epidemiological method that utilizes single nucleotide polymorphisms (SNPs) as instrumental variables (IVs), less susceptible to unmeasured confounders and reverse causality [ 11 ]. This study aims to elucidate potential disease mechanisms, identify novel therapeutic avenues, and provide insights for the diagnosis, treatment, and prevention of GBM.

Materials and methods

Study design.

Our study provides an overview of two sample MR surveys used to explore the causal relationship between CSF metabolites and GBM. In MR studies, adherence to three fundamental instrumental variable (IV) assumptions is crucial: (1) genetic variants must correlate with the exposure; (2) these variants should be independent of confounding factors; and (3) they should exclusively impact the outcome through the exposure [ 11 ]. Strict quality control measures, including tests for multicollinearity and heterogeneity, were conducted to enhance the reliability of causal results.

CSF metabolites data synopsis

The exposure factor we studied is 338 CSF metabolites, based on a metabolome-wide association study by Panyard et al. [ 12 ]. In this study, a total of 291 independent samples of individuals of European descent were retained after quality control and further data cleaning steps. All participants were in a state of cognitive health when CSF was extracted. Among the 338 metabolites, 38 are still chemically undefined, whereas 296 have undergone chemical validation and are categorized into eight primary metabolic groups, encompassing amino acid, carbohydrate, cofactor and vitamin, energy, lipid, nucleotide, peptide, and xenobiotic metabolism.

GBM data synopsis

The outcome variable under scrutiny is GBM, for which the genome-wide association study (GWAS) summary dataset was sourced from FinnGen included 91 cases and 174,006 controls of European descent and comprised 16,380,303 SNPs.

Selection of IVs

SNPs are commonly used genetic variations in MR Analysis, reflecting DNA sequence diversity resulting from a single nucleotide change at the genomic level. In our study, we meticulously constructed a unique set of IVs for each of the 338 CSF metabolites. This approach was taken to ensure that the genetic associations specific to each metabolite were accurately represented. Firstly, considering the limited number of SNPs available to achieve genome-wide significance for metabolites, we relaxed the threshold by testing with a p-value set to less than 1 × 10^ −5 [ 13 ]. Then, linkage disequilibrium criteria were set at r 2  < 0.001 and a genetic distance of 10,000 kb, with highly correlated SNPs excluded to ensure the independence of included SNPs. Furthermore, to mitigate bias resulting from weak instrumentality, the F-value of each SNP was computed, and SNPs with an F-value < 10 were identified as weak instruments [ 14 ]. F = R 2 ( N  − 2)/(1 − R2), in which R 2 and N denotes the proportion of variance explained by the chosen SNPs and the sample size of the GWAS for the SNP respectively. What’s more, we utilized the IEU OpenGWAS database to meticulously identify and exclude any SNPs that showed significant associations with potential confounders. Finally, project the SNPs related to CSF metabolites onto the GWAS merged data of GBM and extract the corresponding statistical parameters.

Statistical analysis

In this study, we employed the Inverse Variance Weighted (IVW), MR-Egger, and Weighted Median, Weighted mode, and Simple mode methods to estimate dependent effects. Given the IVW method’s superior test efficacy compared to other MR methods, we selected it as the primary method. The IVW method assumes the validity of all genetic variants as instrumental variables. It calculates causal effect values for individual instrumental variables using the ratio method, then combines each estimate through weighted linear regression to derive the total effect value. Nonetheless, there might be unidentified confounding variables leading to genetic pleiotropy and bias in effect size estimation. Therefore, MR-Egger regression and weighted median methods were employed as supplementary approaches to corroborate the causal impact of exposure on the outcome. The “leave-one-out” method was employed to identify any instrumental variables that could influence the estimation of causal effects. Horizontal pleiotropy was assessed using the Egger intercept, and the Mendelian randomization pleiotropy residual sum and outlier (MR-PRESSO) test was performed to identify and exclude SNPs that may be influenced by pleiotropy. Heterogeneity tests were conducted using the Cochran Q test to evaluate the diversity of the SNPs. All analyses were performed using the TwoSampleMR R packages in R software 4.4.1.

After investigating the quality control of IVs, we identified 14 CSF metabolites associated with GBM, including 21–196 SNPs (with Stachydrine genetically represented by 21 SNPs and Butyrate (4:0) having the highest representation with 196 SNPs). The F-statistic data range for CSF metabolites is from 19.37 to 78.49, all exceeding a threshold greater than 10, indicating they are less likely to be influenced by instrument biases (Supplementary Table S1 ). Among the 14 CSF metabolites associated with GBM, there are 12 identified metabolites and 2 with unknown chemical properties. The identified metabolites chemically classify into lipids, vitamins, amino acids, nucleotides, energy and xenobiotics (Supplementary Table S2 ).

The causal relationship between the risk of GBM and 14 CSF metabolites is as follows (Fig.  1 ): 1-palmitoyl-2-stearoyl-gpc (16:0/18:0) (OR 1.661, 95% CI: 1.039–2.654, p  = 0.034), 7-alpha-hydroxy-3-oxo-4-cholestenoate (7-HOCA) (OR 4.272, 95% CI: 1.335–13.673, p  = 0.014), Alpha-tocopherol (12:0) (OR 1.693, 95% CI: 1.037–2.764, p  = 0.035), Behenoyl sphingomyelin (d18:1/22:0) (OR 1.515, 95% CI: 1.120–2.051, p  = 0.007), Cysteinylglycine (OR 1.361, 95% CI: 1.072–1.726, p  = 0.011), Maleate (OR 1.517, 95% CI: 1.077–2.137, p  = 0.017), N1-methylinosine (OR 0.467, 95% CI: 0.232–0.941, p  = 0.033), Stachydrine (OR 0.738, 95% CI: 0.578–0.942, p  = 0.015), Succinylcarnitine (c4-dc) (OR 0.328, 95% CI: 0.110–0.978, p  = 0.046), Uracil (OR 5.053, 95% CI: 1.044–24.449, p  = 0.044), Valine (OR 5.284, 95% CI: 1.170–23.868, p  = 0.030), X-12,101 (OR 2.219, 95% CI: 1.148–4.290, p  = 0.018), X-12,104 (OR 2.943, 95% CI: 1.023–8.463, p  = 0.045), Butyrate (4:0) (OR 1.422, 95% CI: 1.063–1.904, p  = 0.018).

Forest plot of mendelian randomization estimates between CSF metabolites and GBM

In brief, the IVW-derived estimates demonstrated significance ( p  < 0.05), with a consistent direction and magnitude observed across IVW, MR-Egger, Weighted mode, Weighted median and Simple mode estimates (Supplementary Table S3 ). The conclusions of the other four methods remain largely consistent with those of the IWV method. Both the MR-Egger intercept test and the Cochran Q test strongly support the absence of pleiotropy and heterogeneity, except the Egger intercept of Behenoyl sphingomyelin (d18:1/22:0) showing pleiotropy (Table  1 ). Scatter plots for 14 identified CSF metabolites across various tests are displayed in Fig.  2 . The leave-one-out analysis confirmed that excluding any single SNP did not introduce bias into the MR estimation (Supplementary Figure S1 ). The funnel plots are presented in Supplementary Figure S2 .

Scatter plots of 14 CSF metabolites related to the risk of GBM

Our study delved into the intricate relationship between CSF metabolites and GBM, shedding light on potential biomarkers and pathways implicated in the pathogenesis of this aggressive brain tumor. By investigating the relationship between CSF metabolites and GBM risk through MR analysis, we identified 14 CSF metabolites significantly associated with the risk of GBM. These metabolites encompass a variety of biochemical classes, including lipids, vitamins, amino acids, nucleotides, energy and xenobiotics, underscoring the multifactorial nature of GBM development and progression. Understanding the dynamic interplay between CSF metabolites and GBM not only enhances our diagnostic capabilities but also holds promise for the development of targeted therapeutic interventions aimed at disrupting key metabolic pathways implicated in GBM tumorigenesis and progression.

Lipids, including sterols, phospholipids, and diacyl/triacylglycerols, are components of biofilms, which emerge as pivotal players in the landscape of GBM research. Lipids serve not only as structural components of cell membranes but also as signaling molecules involved in various cellular processes, including proliferation, migration, and apoptosis. The dysregulation of lipid metabolism has been implicated in tumor initiation, progression, and therapy resistance, making lipid metabolites attractive candidates for biomarker discovery and therapeutic targeting in GBM [ 15 ]. So far, there have been no literature reports on the roles of 1-palmitoyl-2-steroyl-gpc (16:0/18:0) and Behenoyl sphingomyelin (d18:1/22:0) in cancer. We speculate that these two phospholipid metabolites may be associated with enhanced lipid synthesis and metabolism typically exhibited by cancer cells. Not only do they provide the structural support required for cancer cell proliferation, but they also participate in regulating cell signaling pathways and epigenetic events, promoting the survival and metastasis ability of cancer cells. Halama et al. have discovered that in colon and ovarian cancer, endothelial cells induce metabolic reprogramming by promoting the overexpression of glycerophospholipids and polyunsaturated fatty acids in cancer cells [ 16 ].

In our study, we are pioneering the association between 7-HOCA and GBM for the first time. 7-HOCA is the primary metabolite of oxysterol 27-hydroxycholesterol in the brain, and the increase of 7-HOCA in CSF has been shown to reflect blood-brain barrier damage [ 17 , 18 ]. Feng et al. found that 7-HOCA is closely related to the occurrence of lung cancer, showing a significantly increased risk [ 19 ]. Combining our research findings, 7-HOCA may play a significant role in the development of cancer. Butyrate (4:0) is the salt form of butyric acid, which stands as a significant byproduct in fatty acid metabolism. In vivo, butyric acid can be generated through various pathways, including its production from the metabolism of fatty acids or its fermentation from dietary fiber by intestinal microbiota. Once produced, butyric acid actively participates in the regulation and modulation of lipid metabolism, influencing the synthesis, breakdown, and utilization of fats in the body. Tumor cells can obtain fatty acids through lipolysis to support their growth. During periods of adequate nutritional supply, fatty acids are stored in adipose tissue as triglycerides (TG), which are broken down to release fatty acids when energy levels are low. Recent studies have revealed the significant role of butyrate salts in the occurrence and progression of colorectal and lung cancers [ 20 ]. However, this is the first report on the relationship between butyrate salts and GBM. We are also presenting, for the first time, the causal relationship between maleate in CSF and GBM. Maleate is a salt form of malic acid, present in the human body as a metabolite or dietary source. Further research is needed to understand its involvement in the onset and progression of GBM.

The importance of amino acid metabolism in GBM lies in its role as the building blocks of proteins. Amino acids not only provide essential materials for cell growth and proliferation but also participate in regulating cellular signaling, gene expression, and metabolic pathways. GBM cells typically exhibit high metabolic activity, leading to an increased demand for amino acids. Therefore, abnormalities in amino acid supply and metabolism may influence the development and progression of glioblastoma. In our study, valine and cysteinylglycine was found to have a positive relationship with GBM. Similar to our results, Yao et al. found that the valine, leucine, and isoleucine biosynthesis metabolic pathway can be used for screening and diagnosis of ovarian cancer [ 21 ]. Valine, leucine and isoleucine constitute the group of branched-chain amino acids (BCAAs), which can be utilized and processed by astrocytes to perform various functions, including serving as fuel materials for brain energy metabolism [ 22 ]. The diagnostic role of valine metabolites in cancer has also been found in saliva. In another study, valine was found to play a dominant role in diagnosing multiple cancers through saliva [ 23 ]. Cysteinylglycine, produced during the breakdown of glutathione, has been proposed as a prooxidant, contributing to oxidative stress and lipid peroxidation, which are implicated in the development of human cancers. Consistent with our results, Lin et al. found that the increase of cysteinylglycine level was associated with the increased risk of breast cancer in the high oxidative stress group of middle-aged and elderly women [ 24 ]. However, there are also studies suggesting the opposite perspective. For instance, Miranti et al. found a negative correlation between serum cysteinylglycine and esophageal adenocarcinoma [ 25 ]. For understanding and intervening in the metabolic characteristics and therapeutic mechanisms of GBM, in-depth investigation into the role of amino acid metabolism is crucial.

As the fundamental substances that ensure the structure of DNA, the metabolism of nucleotides is closely intertwined with the initiation and progression of tumors. As shown in this study, uracil may act as a metabolite and participant in the onset and progression of tumors. UPP1, as a critical enzyme in uracil metabolism, catalyzes the dephosphorylation of uridine to uracil and ribose-1-phosphate. Its activation of the AKT signaling pathway can enhance tumorigenesis and resistance to anticancer drugs [ 26 ]. N1-methylinosine is a known modification of ribonucleosides, formed by adding a methyl group to the N1 position of the purine ring of a nucleotide. This methylation modification typically occurs within RNA molecules. In biological organisms, methylation modifications of ribonucleosides are common epigenetic regulatory mechanisms that can influence RNA stability, translation, and interactions. Studies by Li et al. detected N1-methylinosine in urine samples from cancer patients, suggesting its potential as a biomarker for cancer [ 27 ]. The presence of N1-methylinosine may indicate abnormalities in ribonucleoside metabolism within cells, which could be associated with the development and progression of cancer.

In our study, except N1-methylinosine, another two metabolites that showed a negative causal relationship with GBM were Stachydrine and Succinylcarnitine (c4-dc). Stachydrine is renowned for its antioxidant and anti-inflammatory properties [ 28 ]. Consistent with our results, some studies have demonstrated that Stachydrine can inhibit the proliferation of breast cancer and liver cancer cells, while inducing apoptosis, autophagy, and cellular senescence. Its mechanisms of action may include inhibiting survival signaling pathways such as Akt and ERK, as well as regulating the expression of cell cycle proteins [ 29 , 30 ]. Liu et al. found that Stachydrine may prevent liver damage by attenuating inflammatory responses, inhibiting the ERK and AKT pathways, and suppressing the expression of macrophage stimulating protein [ 31 ]. Succinylcarnitine is an intermediate in the tricarboxylic acid (TCA) cycle and amino acid-based energy metabolism, responsible for fatty acid β-oxidation and mitochondrial function, serving as an energy-related metabolic product [ 32 ]. An MR study identified maternal succinylcarnitine during pregnancy as a significant factor contributing to the risk of congenital heart disease in offspring [ 33 ]. Kim et al. found that the decrease in plasma succinylcarnitine levels was associated with the inhibition of prostate tumor growth by a whole walnut diet, possibly achieved through influencing cellular energy metabolism status and regulating relevant gene expression [ 34 ]. However, further research is needed to elucidate the specific mechanisms of these two CSF metabolites.

Several limitations exist within our study. the Pleiotropy test result of Behenoyl sphingomyelin (d18:1/22:0) was less than 0.05, indicating the presence of pleiotropy. However, further SNP screening was performed using MR-PRESSO, but the results showed no SNPs that needed to be removed. There are many possible reasons for this result, such as sample size, insufficient statistical power, complexity of pleiotropy, and methodological limitations. Therefore, it is emphasized that readers need to be cautious when reviewing the results, which does not mean that there is no pleiotropy, but may be due to the influence of the above factors. Additionally, our study may have another important limitation, which is the lack of research on the potential correlation of metabolites at SNP and phenotype levels. This oversight may have implications for the interpretation of our findings, as unaccounted correlations could influence the results of the Mendelian Randomization analysis.

These findings from our study open several avenues for future research. It is essential to delve into the mechanistic roles of the identified CSF metabolites in GBM biology, exploring their interactions with cellular pathways and the tumor microenvironment. Additionally, longitudinal studies are needed to understand the dynamics of these metabolites over the course of the disease and in response to treatments. Investigating the generalizability of our findings across diverse populations and the integration of metabolomics data with other omics platforms will be critical for identifying robust biomarker panels. Furthermore, the potential of these metabolites as therapeutic targets should be rigorously tested in preclinical models to advance towards novel treatment strategies for GBM.

In conclusion, our study provides valuable insights into the intricate relationship between CSF metabolites and GBM, shedding light on potential biomarkers and pathways involved in the pathogenesis of this aggressive brain tumor. Through MR analysis, we identified 14 CSF metabolites significantly associated with GBM risk, spanning various biochemical classes such as lipids, vitamins, amino acids, nucleotides, energy, and xenobiotics. This underscores the multifactorial nature of GBM development and progression. Our findings highlight the importance of understanding the dynamic interplay between CSF metabolites and GBM, not only for enhancing diagnostic capabilities but also for potentially developing targeted therapeutic interventions aimed at disrupting key metabolic pathways implicated in GBM tumorigenesis and progression.

Data availability

The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.

Abbreviations

7-alpha-hydroxy-3-oxo-4-cholestenoate

• Cerebrospinal fluid

Glioblastoma multiforme

Genome-wide association study

Instrumental variables

• Mendelian randomization

Single nucleotide polymorphisms

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Acknowledgements

The authors thank all the participants and researchers who contributed and collected data.

This research was supported by Research Foundation for Talented Scholars of Xuzhou Medical University (D2021011).

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Haijun Bao, Yiyang Chen, Zijun Meng & Zheng Chu

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HjB: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Projection administration; Software, Validation, Writing – original draft. YyC: Formal analysis, Investigation, Writing – original draft. ZjM: Conceptualization, Data curation, Investigation, Writing – review & editing. ZC: Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Projection administration; Software, Validation, Writing – review & editing.

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Bao, H., Chen, Y., Meng, Z. et al. The causal relationship between CSF metabolites and GBM: a two-sample mendelian randomization analysis. BMC Cancer 24 , 1119 (2024). https://doi.org/10.1186/s12885-024-12901-7

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Received : 25 March 2024

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DOI : https://doi.org/10.1186/s12885-024-12901-7

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