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The Beginner's Guide to Statistical Analysis | 5 Steps & Examples

Statistical analysis means investigating trends, patterns, and relationships using quantitative data . It is an important research tool used by scientists, governments, businesses, and other organizations.

To draw valid conclusions, statistical analysis requires careful planning from the very start of the research process . You need to specify your hypotheses and make decisions about your research design, sample size, and sampling procedure.

After collecting data from your sample, you can organize and summarize the data using descriptive statistics . Then, you can use inferential statistics to formally test hypotheses and make estimates about the population. Finally, you can interpret and generalize your findings.

This article is a practical introduction to statistical analysis for students and researchers. We’ll walk you through the steps using two research examples. The first investigates a potential cause-and-effect relationship, while the second investigates a potential correlation between variables.

Table of contents

Step 1: write your hypotheses and plan your research design, step 2: collect data from a sample, step 3: summarize your data with descriptive statistics, step 4: test hypotheses or make estimates with inferential statistics, step 5: interpret your results, other interesting articles.

To collect valid data for statistical analysis, you first need to specify your hypotheses and plan out your research design.

Writing statistical hypotheses

The goal of research is often to investigate a relationship between variables within a population . You start with a prediction, and use statistical analysis to test that prediction.

A statistical hypothesis is a formal way of writing a prediction about a population. Every research prediction is rephrased into null and alternative hypotheses that can be tested using sample data.

While the null hypothesis always predicts no effect or no relationship between variables, the alternative hypothesis states your research prediction of an effect or relationship.

• Null hypothesis: A 5-minute meditation exercise will have no effect on math test scores in teenagers.
• Alternative hypothesis: A 5-minute meditation exercise will improve math test scores in teenagers.
• Null hypothesis: Parental income and GPA have no relationship with each other in college students.
• Alternative hypothesis: Parental income and GPA are positively correlated in college students.

Planning your research design

A research design is your overall strategy for data collection and analysis. It determines the statistical tests you can use to test your hypothesis later on.

First, decide whether your research will use a descriptive, correlational, or experimental design. Experiments directly influence variables, whereas descriptive and correlational studies only measure variables.

• In an experimental design , you can assess a cause-and-effect relationship (e.g., the effect of meditation on test scores) using statistical tests of comparison or regression.
• In a correlational design , you can explore relationships between variables (e.g., parental income and GPA) without any assumption of causality using correlation coefficients and significance tests.
• In a descriptive design , you can study the characteristics of a population or phenomenon (e.g., the prevalence of anxiety in U.S. college students) using statistical tests to draw inferences from sample data.

Your research design also concerns whether you’ll compare participants at the group level or individual level, or both.

• In a between-subjects design , you compare the group-level outcomes of participants who have been exposed to different treatments (e.g., those who performed a meditation exercise vs those who didn’t).
• In a within-subjects design , you compare repeated measures from participants who have participated in all treatments of a study (e.g., scores from before and after performing a meditation exercise).
• In a mixed (factorial) design , one variable is altered between subjects and another is altered within subjects (e.g., pretest and posttest scores from participants who either did or didn’t do a meditation exercise).
• Experimental
• Correlational

First, you’ll take baseline test scores from participants. Then, your participants will undergo a 5-minute meditation exercise. Finally, you’ll record participants’ scores from a second math test.

In this experiment, the independent variable is the 5-minute meditation exercise, and the dependent variable is the math test score from before and after the intervention. Example: Correlational research design In a correlational study, you test whether there is a relationship between parental income and GPA in graduating college students. To collect your data, you will ask participants to fill in a survey and self-report their parents’ incomes and their own GPA.

Measuring variables

When planning a research design, you should operationalize your variables and decide exactly how you will measure them.

For statistical analysis, it’s important to consider the level of measurement of your variables, which tells you what kind of data they contain:

• Categorical data represents groupings. These may be nominal (e.g., gender) or ordinal (e.g. level of language ability).
• Quantitative data represents amounts. These may be on an interval scale (e.g. test score) or a ratio scale (e.g. age).

Many variables can be measured at different levels of precision. For example, age data can be quantitative (8 years old) or categorical (young). If a variable is coded numerically (e.g., level of agreement from 1–5), it doesn’t automatically mean that it’s quantitative instead of categorical.

Identifying the measurement level is important for choosing appropriate statistics and hypothesis tests. For example, you can calculate a mean score with quantitative data, but not with categorical data.

In a research study, along with measures of your variables of interest, you’ll often collect data on relevant participant characteristics.

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In most cases, it’s too difficult or expensive to collect data from every member of the population you’re interested in studying. Instead, you’ll collect data from a sample.

Statistical analysis allows you to apply your findings beyond your own sample as long as you use appropriate sampling procedures . You should aim for a sample that is representative of the population.

Sampling for statistical analysis

There are two main approaches to selecting a sample.

• Probability sampling: every member of the population has a chance of being selected for the study through random selection.
• Non-probability sampling: some members of the population are more likely than others to be selected for the study because of criteria such as convenience or voluntary self-selection.

In theory, for highly generalizable findings, you should use a probability sampling method. Random selection reduces several types of research bias , like sampling bias , and ensures that data from your sample is actually typical of the population. Parametric tests can be used to make strong statistical inferences when data are collected using probability sampling.

But in practice, it’s rarely possible to gather the ideal sample. While non-probability samples are more likely to at risk for biases like self-selection bias , they are much easier to recruit and collect data from. Non-parametric tests are more appropriate for non-probability samples, but they result in weaker inferences about the population.

If you want to use parametric tests for non-probability samples, you have to make the case that:

• your sample is representative of the population you’re generalizing your findings to.
• your sample lacks systematic bias.

Keep in mind that external validity means that you can only generalize your conclusions to others who share the characteristics of your sample. For instance, results from Western, Educated, Industrialized, Rich and Democratic samples (e.g., college students in the US) aren’t automatically applicable to all non-WEIRD populations.

If you apply parametric tests to data from non-probability samples, be sure to elaborate on the limitations of how far your results can be generalized in your discussion section .

Create an appropriate sampling procedure

Based on the resources available for your research, decide on how you’ll recruit participants.

• Will you have resources to advertise your study widely, including outside of your university setting?
• Will you have the means to recruit a diverse sample that represents a broad population?
• Do you have time to contact and follow up with members of hard-to-reach groups?

Your participants are self-selected by their schools. Although you’re using a non-probability sample, you aim for a diverse and representative sample. Example: Sampling (correlational study) Your main population of interest is male college students in the US. Using social media advertising, you recruit senior-year male college students from a smaller subpopulation: seven universities in the Boston area.

Calculate sufficient sample size

Before recruiting participants, decide on your sample size either by looking at other studies in your field or using statistics. A sample that’s too small may be unrepresentative of the sample, while a sample that’s too large will be more costly than necessary.

There are many sample size calculators online. Different formulas are used depending on whether you have subgroups or how rigorous your study should be (e.g., in clinical research). As a rule of thumb, a minimum of 30 units or more per subgroup is necessary.

To use these calculators, you have to understand and input these key components:

• Significance level (alpha): the risk of rejecting a true null hypothesis that you are willing to take, usually set at 5%.
• Statistical power : the probability of your study detecting an effect of a certain size if there is one, usually 80% or higher.
• Expected effect size : a standardized indication of how large the expected result of your study will be, usually based on other similar studies.
• Population standard deviation: an estimate of the population parameter based on a previous study or a pilot study of your own.

Once you’ve collected all of your data, you can inspect them and calculate descriptive statistics that summarize them.

Inspect your data

There are various ways to inspect your data, including the following:

• Organizing data from each variable in frequency distribution tables .
• Displaying data from a key variable in a bar chart to view the distribution of responses.
• Visualizing the relationship between two variables using a scatter plot .

By visualizing your data in tables and graphs, you can assess whether your data follow a skewed or normal distribution and whether there are any outliers or missing data.

A normal distribution means that your data are symmetrically distributed around a center where most values lie, with the values tapering off at the tail ends.

In contrast, a skewed distribution is asymmetric and has more values on one end than the other. The shape of the distribution is important to keep in mind because only some descriptive statistics should be used with skewed distributions.

Extreme outliers can also produce misleading statistics, so you may need a systematic approach to dealing with these values.

Calculate measures of central tendency

Measures of central tendency describe where most of the values in a data set lie. Three main measures of central tendency are often reported:

• Mode : the most popular response or value in the data set.
• Median : the value in the exact middle of the data set when ordered from low to high.
• Mean : the sum of all values divided by the number of values.

However, depending on the shape of the distribution and level of measurement, only one or two of these measures may be appropriate. For example, many demographic characteristics can only be described using the mode or proportions, while a variable like reaction time may not have a mode at all.

Calculate measures of variability

Measures of variability tell you how spread out the values in a data set are. Four main measures of variability are often reported:

• Range : the highest value minus the lowest value of the data set.
• Interquartile range : the range of the middle half of the data set.
• Standard deviation : the average distance between each value in your data set and the mean.
• Variance : the square of the standard deviation.

Once again, the shape of the distribution and level of measurement should guide your choice of variability statistics. The interquartile range is the best measure for skewed distributions, while standard deviation and variance provide the best information for normal distributions.

Using your table, you should check whether the units of the descriptive statistics are comparable for pretest and posttest scores. For example, are the variance levels similar across the groups? Are there any extreme values? If there are, you may need to identify and remove extreme outliers in your data set or transform your data before performing a statistical test.

From this table, we can see that the mean score increased after the meditation exercise, and the variances of the two scores are comparable. Next, we can perform a statistical test to find out if this improvement in test scores is statistically significant in the population. Example: Descriptive statistics (correlational study) After collecting data from 653 students, you tabulate descriptive statistics for annual parental income and GPA.

It’s important to check whether you have a broad range of data points. If you don’t, your data may be skewed towards some groups more than others (e.g., high academic achievers), and only limited inferences can be made about a relationship.

A number that describes a sample is called a statistic , while a number describing a population is called a parameter . Using inferential statistics , you can make conclusions about population parameters based on sample statistics.

Researchers often use two main methods (simultaneously) to make inferences in statistics.

• Estimation: calculating population parameters based on sample statistics.
• Hypothesis testing: a formal process for testing research predictions about the population using samples.

You can make two types of estimates of population parameters from sample statistics:

• A point estimate : a value that represents your best guess of the exact parameter.
• An interval estimate : a range of values that represent your best guess of where the parameter lies.

If your aim is to infer and report population characteristics from sample data, it’s best to use both point and interval estimates in your paper.

You can consider a sample statistic a point estimate for the population parameter when you have a representative sample (e.g., in a wide public opinion poll, the proportion of a sample that supports the current government is taken as the population proportion of government supporters).

There’s always error involved in estimation, so you should also provide a confidence interval as an interval estimate to show the variability around a point estimate.

A confidence interval uses the standard error and the z score from the standard normal distribution to convey where you’d generally expect to find the population parameter most of the time.

Hypothesis testing

Using data from a sample, you can test hypotheses about relationships between variables in the population. Hypothesis testing starts with the assumption that the null hypothesis is true in the population, and you use statistical tests to assess whether the null hypothesis can be rejected or not.

Statistical tests determine where your sample data would lie on an expected distribution of sample data if the null hypothesis were true. These tests give two main outputs:

• A test statistic tells you how much your data differs from the null hypothesis of the test.
• A p value tells you the likelihood of obtaining your results if the null hypothesis is actually true in the population.

Statistical tests come in three main varieties:

• Comparison tests assess group differences in outcomes.
• Regression tests assess cause-and-effect relationships between variables.
• Correlation tests assess relationships between variables without assuming causation.

Your choice of statistical test depends on your research questions, research design, sampling method, and data characteristics.

Parametric tests

Parametric tests make powerful inferences about the population based on sample data. But to use them, some assumptions must be met, and only some types of variables can be used. If your data violate these assumptions, you can perform appropriate data transformations or use alternative non-parametric tests instead.

A regression models the extent to which changes in a predictor variable results in changes in outcome variable(s).

• A simple linear regression includes one predictor variable and one outcome variable.
• A multiple linear regression includes two or more predictor variables and one outcome variable.

Comparison tests usually compare the means of groups. These may be the means of different groups within a sample (e.g., a treatment and control group), the means of one sample group taken at different times (e.g., pretest and posttest scores), or a sample mean and a population mean.

• A t test is for exactly 1 or 2 groups when the sample is small (30 or less).
• A z test is for exactly 1 or 2 groups when the sample is large.
• An ANOVA is for 3 or more groups.

The z and t tests have subtypes based on the number and types of samples and the hypotheses:

• If you have only one sample that you want to compare to a population mean, use a one-sample test .
• If you have paired measurements (within-subjects design), use a dependent (paired) samples test .
• If you have completely separate measurements from two unmatched groups (between-subjects design), use an independent (unpaired) samples test .
• If you expect a difference between groups in a specific direction, use a one-tailed test .
• If you don’t have any expectations for the direction of a difference between groups, use a two-tailed test .

The only parametric correlation test is Pearson’s r . The correlation coefficient ( r ) tells you the strength of a linear relationship between two quantitative variables.

However, to test whether the correlation in the sample is strong enough to be important in the population, you also need to perform a significance test of the correlation coefficient, usually a t test, to obtain a p value. This test uses your sample size to calculate how much the correlation coefficient differs from zero in the population.

You use a dependent-samples, one-tailed t test to assess whether the meditation exercise significantly improved math test scores. The test gives you:

• a t value (test statistic) of 3.00
• a p value of 0.0028

Although Pearson’s r is a test statistic, it doesn’t tell you anything about how significant the correlation is in the population. You also need to test whether this sample correlation coefficient is large enough to demonstrate a correlation in the population.

A t test can also determine how significantly a correlation coefficient differs from zero based on sample size. Since you expect a positive correlation between parental income and GPA, you use a one-sample, one-tailed t test. The t test gives you:

• a t value of 3.08
• a p value of 0.001

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The final step of statistical analysis is interpreting your results.

Statistical significance

In hypothesis testing, statistical significance is the main criterion for forming conclusions. You compare your p value to a set significance level (usually 0.05) to decide whether your results are statistically significant or non-significant.

Statistically significant results are considered unlikely to have arisen solely due to chance. There is only a very low chance of such a result occurring if the null hypothesis is true in the population.

This means that you believe the meditation intervention, rather than random factors, directly caused the increase in test scores. Example: Interpret your results (correlational study) You compare your p value of 0.001 to your significance threshold of 0.05. With a p value under this threshold, you can reject the null hypothesis. This indicates a statistically significant correlation between parental income and GPA in male college students.

Note that correlation doesn’t always mean causation, because there are often many underlying factors contributing to a complex variable like GPA. Even if one variable is related to another, this may be because of a third variable influencing both of them, or indirect links between the two variables.

Effect size

A statistically significant result doesn’t necessarily mean that there are important real life applications or clinical outcomes for a finding.

In contrast, the effect size indicates the practical significance of your results. It’s important to report effect sizes along with your inferential statistics for a complete picture of your results. You should also report interval estimates of effect sizes if you’re writing an APA style paper .

With a Cohen’s d of 0.72, there’s medium to high practical significance to your finding that the meditation exercise improved test scores. Example: Effect size (correlational study) To determine the effect size of the correlation coefficient, you compare your Pearson’s r value to Cohen’s effect size criteria.

Decision errors

Type I and Type II errors are mistakes made in research conclusions. A Type I error means rejecting the null hypothesis when it’s actually true, while a Type II error means failing to reject the null hypothesis when it’s false.

You can aim to minimize the risk of these errors by selecting an optimal significance level and ensuring high power . However, there’s a trade-off between the two errors, so a fine balance is necessary.

Frequentist versus Bayesian statistics

Traditionally, frequentist statistics emphasizes null hypothesis significance testing and always starts with the assumption of a true null hypothesis.

However, Bayesian statistics has grown in popularity as an alternative approach in the last few decades. In this approach, you use previous research to continually update your hypotheses based on your expectations and observations.

Bayes factor compares the relative strength of evidence for the null versus the alternative hypothesis rather than making a conclusion about rejecting the null hypothesis or not.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Student’s  t -distribution
• Normal distribution
• Null and Alternative Hypotheses
• Chi square tests
• Confidence interval

Methodology

• Cluster sampling
• Stratified sampling
• Data cleansing
• Reproducibility vs Replicability
• Peer review
• Likert scale

Research bias

• Implicit bias
• Framing effect
• Cognitive bias
• Placebo effect
• Hawthorne effect
• Hostile attribution bias
• Affect heuristic

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Statistical Methods in Theses: Guidelines and Explanations

Signed August 2018 Naseem Al-Aidroos, PhD, Christopher Fiacconi, PhD Deborah Powell, PhD, Harvey Marmurek, PhD, Ian Newby-Clark, PhD, Jeffrey Spence, PhD, David Stanley, PhD, Lana Trick, PhD

Version:  2.00

This document is an organizational aid, and workbook, for students. We encourage students to take this document to meetings with their advisor and committee. This guide should enhance a committee’s ability to assess key areas of a student’s work. 

In recent years a number of well-known and apparently well-established findings have  failed to replicate , resulting in what is commonly referred to as the replication crisis. The APA Publication Manual 6 th Edition notes that “The essence of the scientific method involves observations that can be repeated and verified by others.” (p. 12). However, a systematic investigation of the replicability of psychology findings published in  Science  revealed that over half of psychology findings do not replicate (see a related commentary in  Nature ). Even more disturbing, a  Bayesian reanalysis of the reproducibility project  showed that 64% of studies had sample sizes so small that strong evidence for or against the null or alternative hypotheses did not exist. Indeed, Morey and Lakens (2016) concluded that most of psychology is statistically unfalsifiable due to small sample sizes and correspondingly low power (see  article ). Our discipline’s reputation is suffering. News of the replication crisis has reached the popular press (e.g.,  The Atlantic ,   The Economist ,   Slate , Last Week Tonight ).

An increasing number of psychologists have responded by promoting new research standards that involve open science and the elimination of  Questionable Research Practices . The open science perspective is made manifest in the  Transparency and Openness Promotion (TOP) guidelines  for journal publications. These guidelines were adopted some time ago by the  Association for Psychological Science . More recently, the guidelines were adopted by American Psychological Association journals ( see details ) and journals published by Elsevier ( see details ). It appears likely that, in the very near future, most journals in psychology will be using an open science approach. We strongly advise readers to take a moment to inspect the  TOP Guidelines Summary Table . 

A key aspect of open science and the TOP guidelines is the sharing of data associated with published research (with respect to medical research, see point #35 in the  World Medical Association Declaration of Helsinki ). This practice is viewed widely as highly important. Indeed, open science is recommended by  all G7 science ministers . All Tri-Agency grants must include a data-management plan that includes plans for sharing: “ research data resulting from agency funding should normally be preserved in a publicly accessible, secure and curated repository or other platform for discovery and reuse by others.”  Moreover, a 2017 editorial published in the  New England Journal of Medicine announced that the  International Committee of Medical Journal Editors believes there is  “an ethical obligation to responsibly share data.”  As of this writing,  60% of highly ranked psychology journals require or encourage data sharing .

The increasing importance of demonstrating that findings are replicable is reflected in calls to make replication a requirement for the promotion of faculty (see details in  Nature ) and experts in open science are now refereeing applications for tenure and promotion (see details at the  Center for Open Science  and  this article ). Most dramatically, in one instance, a paper resulting from a dissertation was retracted due to misleading findings attributable to Questionable Research Practices. Subsequent to the retraction, the Ohio State University’s Board of Trustees unanimously revoked the PhD of the graduate student who wrote the dissertation ( see details ). Thus, the academic environment is changing and it is important to work toward using new best practices in lieu of older practices—many of which are synonymous with Questionable Research Practices. Doing so should help you avoid later career regrets and subsequent  public mea culpas . One way to achieve your research objectives in this new academic environment is  to incorporate replications into your research . Replications are becoming more common and there are even websites dedicated to helping students conduct replications (e.g.,  Psychology Science Accelerator ) and indexing the success of replications (e.g., Curate Science ). You might even consider conducting a replication for your thesis (subject to committee approval).

As early-career researchers, it is important to be aware of the changing academic environment. Senior principal investigators may be  reluctant to engage in open science  (see this student perspective in a  blog post  and  podcast ) and research on resistance to data sharing indicates that one of the barriers to sharing data is that researchers do not feel that they have knowledge of  how to share data online . This document is an educational aid and resource to provide students with introductory knowledge of how to participate in open science and online data sharing to start their education on these subjects. 

Guidelines and Explanations

In light of the changes in psychology, faculty members who teach statistics/methods have reviewed the literature and generated this guide for graduate students. The guide is intended to enhance the quality of student theses by facilitating their engagement in open and transparent research practices and by helping them avoid Questionable Research Practices, many of which are now deemed unethical and covered in the ethics section of textbooks.

This document is an informational tool.

How to Start

In order to follow best practices, some first steps need to be followed. Here is a list of things to do:

• Get an Open Science account. Registration at  osf.io  is easy!
• If conducting confirmatory hypothesis testing for your thesis, pre-register your hypotheses (see Section 1-Hypothesizing). The Open Science Foundation website has helpful  tutorials  and  guides  to get you going.
• Also, pre-register your data analysis plan. Pre-registration typically includes how and when you will stop collecting data, how you will deal with violations of statistical assumptions and points of influence (“outliers”), the specific measures you will use, and the analyses you will use to test each hypothesis, possibly including the analysis script. Again, there is a lot of help available for this. 

Exploratory and Confirmatory Research Are Both of Value, But Do Not Confuse the Two

We note that this document largely concerns confirmatory research (i.e., testing hypotheses). We by no means intend to devalue exploratory research. Indeed, it is one of the primary ways that hypotheses are generated for (possible) confirmation. Instead, we emphasize that it is important that you clearly indicate what of your research is exploratory and what is confirmatory. Be clear in your writing and in your preregistration plan. You should explicitly indicate which of your analyses are exploratory and which are confirmatory. Please note also that if you are engaged in exploratory research, then Null Hypothesis Significance Testing (NHST) should probably be avoided (see rationale in  Gigerenzer  (2004) and  Wagenmakers et al., (2012) ). 

This document is structured around the stages of thesis work:  hypothesizing, design, data collection, analyses, and reporting – consistent with the headings used by Wicherts et al. (2016). We also list the Questionable Research Practices associated with each stage and provide suggestions for avoiding them. We strongly advise going through all of these sections during thesis/dissertation proposal meetings because a priori decisions need to be made prior to data collection (including analysis decisions). 

To help to ensure that the student has informed the committee about key decisions at each stage, there are check boxes at the end of each section.

How to Use This Document in a Proposal Meeting

• Print off a copy of this document and take it to the proposal meeting.
• During the meeting, use the document to seek assistance from faculty to address potential problems.
• Revisit responses to issues raised by this document (especially the Analysis and Reporting Stages) when you are seeking approval to proceed to defense.

Consultation and Help Line

Note that the Center for Open Science now has a help line (for individual researchers and labs) you can call for help with open science issues. They also have training workshops. Please see their  website  for details.

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International Students blog

Thesis life: 7 ways to tackle statistics in your thesis.

By Pranav Kulkarni

Thesis is an integral part of your Masters’ study in Wageningen University and Research. It is the most exciting, independent and technical part of the study. More often than not, most departments in WU expect students to complete a short term independent project or a part of big on-going project for their thesis assignment.

Source : www.coursera.org

This assignment involves proposing a research question, tackling it with help of some observations or experiments, analyzing these observations or results and then stating them by drawing some conclusions.

Since it is an immitigable part of your thesis, you can neither run from statistics nor cry for help.

The penultimate part of this process involves analysis of results which is very crucial for coherence of your thesis assignment.This analysis usually involve use of statistical tools to help draw inferences. Most students who don’t pursue statistics in their curriculum are scared by this prospect. Since it is an immitigable part of your thesis, you can neither run from statistics nor cry for help. But in order to not get intimidated by statistics and its “greco-latin” language, there are a few ways in which you can make your journey through thesis life a pleasant experience.

Make statistics your friend

The best way to end your fear of statistics and all its paraphernalia is to befriend it. Try to learn all that you can about the techniques that you will be using, why they were invented, how they were invented and who did this deed. Personifying the story of statistical techniques makes them digestible and easy to use. Each new method in statistics comes with a unique story and loads of nerdy anecdotes.

If you cannot make friends with statistics, at least make a truce

If you cannot still bring yourself about to be interested in the life and times of statistics, the best way to not hate statistics is to make an agreement with yourself. You must realise that although important, this is only part of your thesis. The better part of your thesis is something you trained for and learned. So, don’t bother to fuss about statistics and make you all nervous. Do your job, enjoy thesis to the fullest and complete the statistical section as soon as possible. At the end, you would have forgotten all about your worries and fears of statistics.

Visualize your data

The best way to understand the results and observations from your study/ experiments, is to visualize your data. See different trends, patterns, or lack thereof to understand what you are supposed to do. Moreover, graphics and illustrations can be used directly in your report. These techniques will also help you decide on which statistical analyses you must perform to answer your research question. Blind decisions about statistics can often influence your study and make it very confusing or worse, make it completely wrong!

Simplify with flowcharts and planning

Similar to graphical visualizations, making flowcharts and planning various steps of your study can prove beneficial to make statistical decisions. Human brain can analyse pictorial information faster than literal information. So, it is always easier to understand your exact goal when you can make decisions based on flowchart or any logical flow-plans.

Source: www.imindq.com

Find examples on internet

Although statistics is a giant maze of complicated terminologies, the internet holds the key to this particular maze. You can find tons of examples on the web. These may be similar to what you intend to do or be different applications of the similar tools that you wish to engage. Especially, in case of Statistical programming languages like R, SAS, Python, PERL, VBA, etc. there is a vast database of example codes, clarifications and direct training examples available on the internet. Various forums are also available for specialized statistical methodologies where different experts and students discuss the issues regarding their own projects.

Comparative studies

Much unlike blindly searching the internet for examples and taking word of advice from online faceless people, you can systematically learn which quantitative tests to perform by rigorously studying literature of relevant research. Since you came up with a certain problem to tackle in your field of study, chances are, someone else also came up with this issue or something quite similar. You can find solutions to many such problems by scouring the internet for research papers which address the issue. Nevertheless, you should be cautious. It is easy to get lost and disheartened when you find many heavy statistical studies with lots of maths and derivations with huge cryptic symbolical text.

When all else fails, talk to an expert

All the steps above are meant to help you independently tackle whatever hurdles you encounter over the course of your thesis. But, when you cannot tackle them yourself it is always prudent and most efficient to ask for help. Talking to students from your thesis ring who have done something similar is one way of help. Another is to make an appointment with your supervisor and take specific questions to him/ her. If that is not possible, you can contact some other teaching staff or researchers from your research group. Try not to waste their as well as you time by making a list of specific problems that you will like to discuss. I think most are happy to help in any way possible.

Talking to students from your thesis ring who have done something similar is one way of help.

Sometimes, with the help of your supervisor, you can make an appointment with someone from the “Biometris” which is the WU’s statistics department. These people are the real deal; chances are, these people can solve all your problems without any difficulty. Always remember, you are in the process of learning, nobody expects you to be an expert in everything. Ask for help when there seems to be no hope.

Apart from these seven ways to make your statistical journey pleasant, you should always engage in reading, watching, listening to stuff relevant to your thesis topic and talking about it to those who are interested. Most questions have solutions in the ether realm of communication. So, best of luck and break a leg!!!

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There are 4 comments.

A perfect approach in a very crisp and clear manner! The sequence suggested is absolutely perfect and will help the students very much. I particularly liked the idea of visualisation!

You are write! I get totally stuck with learning and understanding statistics for my Dissertation!

Statistics is a technical subject that requires extra effort. With the highlighted tips you already highlighted i expect it will offer the much needed help with statistics analysis in my course.

this is so much relevant to me! Don’t forget one more point: try to enrol specific online statistics course (in my case, I’m too late to join any statistic course). The hardest part for me actually to choose what type of statistical test to choose among many options

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• v.25(Suppl 2); 2021 May

An Introduction to Statistics: Choosing the Correct Statistical Test

Priya ranganathan.

1 Department of Anaesthesiology, Critical Care and Pain, Tata Memorial Centre, Homi Bhabha National Institute, Mumbai, Maharashtra, India

The choice of statistical test used for analysis of data from a research study is crucial in interpreting the results of the study. This article gives an overview of the various factors that determine the selection of a statistical test and lists some statistical testsused in common practice.

How to cite this article: Ranganathan P. An Introduction to Statistics: Choosing the Correct Statistical Test. Indian J Crit Care Med 2021;25(Suppl 2):S184–S186.

In a previous article in this series, we looked at different types of data and ways to summarise them. 1 At the end of the research study, statistical analyses are performed to test the hypothesis and either prove or disprove it. The choice of statistical test needs to be carefully performed since the use of incorrect tests could lead to misleading conclusions. Some key questions help us to decide the type of statistical test to be used for analysis of study data. 2

W hat is the R esearch H ypothesis ?

Sometimes, a study may just describe the characteristics of the sample, e.g., a prevalence study. Here, the statistical analysis involves only descriptive statistics . For example, Sridharan et al. aimed to analyze the clinical profile, species distribution, and susceptibility pattern of patients with invasive candidiasis. 3 They used descriptive statistics to express the characteristics of their study sample, including mean (and standard deviation) for normally distributed data, median (with interquartile range) for skewed data, and percentages for categorical data.

Studies may be conducted to test a hypothesis and derive inferences from the sample results to the population. This is known as inferential statistics . The goal of inferential statistics may be to assess differences between groups (comparison), establish an association between two variables (correlation), predict one variable from another (regression), or look for agreement between measurements (agreement). Studies may also look at time to a particular event, analyzed using survival analysis.

A re the C omparisons M atched (P aired ) or U nmatched (U npaired )?

Observations made on the same individual (before–after or comparing two sides of the body) are usually matched or paired . Comparisons made between individuals are usually unpaired or unmatched . Data are considered paired if the values in one set of data are likely to be influenced by the other set (as can happen in before and after readings from the same individual). Examples of paired data include serial measurements of procalcitonin in critically ill patients or comparison of pain relief during sequential administration of different analgesics in a patient with osteoarthritis.

W hat are the T ype of D ata B eing M easured ?

The test chosen to analyze data will depend on whether the data are categorical (and whether nominal or ordinal) or numerical (and whether skewed or normally distributed). Tests used to analyze normally distributed data are known as parametric tests and have a nonparametric counterpart that is used for data, which is distribution-free. 4 Parametric tests assume that the sample data are normally distributed and have the same characteristics as the population; nonparametric tests make no such assumptions. Parametric tests are more powerful and have a greater ability to pick up differences between groups (where they exist); in contrast, nonparametric tests are less efficient at identifying significant differences. Time-to-event data requires a special type of analysis, known as survival analysis.

H ow M any M easurements are B eing C ompared ?

The choice of the test differs depending on whether two or more than two measurements are being compared. This includes more than two groups (unmatched data) or more than two measurements in a group (matched data).

T ests for C omparison

( Table 1 lists the tests commonly used for comparing unpaired data, depending on the number of groups and type of data. As an example, Megahed and colleagues evaluated the role of early bronchoscopy in mechanically ventilated patients with aspiration pneumonitis. 5 Patients were randomized to receive either early bronchoscopy or conventional treatment. Between groups, comparisons were made using the unpaired t test for normally distributed continuous variables, the Mann–Whitney U -test for non-normal continuous variables, and the chi-square test for categorical variables. Chowhan et al. compared the efficacy of left ventricular outflow tract velocity time integral (LVOTVTI) and carotid artery velocity time integral (CAVTI) as predictors of fluid responsiveness in patients with sepsis and septic shock. 6 Patients were divided into three groups— sepsis, septic shock, and controls. Since there were three groups, comparisons of numerical variables were done using analysis of variance (for normally distributed data) or Kruskal–Wallis test (for skewed data).

Tests for comparison of unpaired data

A common error is to use multiple unpaired t -tests for comparing more than two groups; i.e., for a study with three treatment groups A, B, and C, it would be incorrect to run unpaired t -tests for group A vs B, B vs C, and C vs A. The correct technique of analysis is to run ANOVA and use post hoc tests (if ANOVA yields a significant result) to determine which group is different from the others.

( Table 2 lists the tests commonly used for comparing paired data, depending on the number of groups and type of data. As discussed above, it would be incorrect to use multiple paired t -tests to compare more than two measurements within a group. In the study by Chowhan, each parameter (LVOTVTI and CAVTI) was measured in the supine position and following passive leg raise. These represented paired readings from the same individual and comparison of prereading and postreading was performed using the paired t -test. 6 Verma et al. evaluated the role of physiotherapy on oxygen requirements and physiological parameters in patients with COVID-19. 7 Each patient had pretreatment and post-treatment data for heart rate and oxygen supplementation recorded on day 1 and day 14. Since data did not follow a normal distribution, they used Wilcoxon's matched pair test to compare the prevalues and postvalues of heart rate (numerical variable). McNemar's test was used to compare the presupplemental and postsupplemental oxygen status expressed as dichotomous data in terms of yes/no. In the study by Megahed, patients had various parameters such as sepsis-related organ failure assessment score, lung injury score, and clinical pulmonary infection score (CPIS) measured at baseline, on day 3 and day 7. 5 Within groups, comparisons were made using repeated measures ANOVA for normally distributed data and Friedman's test for skewed data.

Tests for comparison of paired data

T ests for A ssociation between V ariables

( Table 3 lists the tests used to determine the association between variables. Correlation determines the strength of the relationship between two variables; regression allows the prediction of one variable from another. Tyagi examined the correlation between ETCO 2 and PaCO 2 in patients with chronic obstructive pulmonary disease with acute exacerbation, who were mechanically ventilated. 8 Since these were normally distributed variables, the linear correlation between ETCO 2 and PaCO 2 was determined by Pearson's correlation coefficient. Parajuli et al. compared the acute physiology and chronic health evaluation II (APACHE II) and acute physiology and chronic health evaluation IV (APACHE IV) scores to predict intensive care unit mortality, both of which were ordinal data. Correlation between APACHE II and APACHE IV score was tested using Spearman's coefficient. 9 A study by Roshan et al. identified risk factors for the development of aspiration pneumonia following rapid sequence intubation. 10 Since the outcome was categorical binary data (aspiration pneumonia— yes/no), they performed a bivariate analysis to derive unadjusted odds ratios, followed by a multivariable logistic regression analysis to calculate adjusted odds ratios for risk factors associated with aspiration pneumonia.

Tests for assessing the association between variables

T ests for A greement between M easurements

( Table 4 outlines the tests used for assessing agreement between measurements. Gunalan evaluated concordance between the National Healthcare Safety Network surveillance criteria and CPIS for the diagnosis of ventilator-associated pneumonia. 11 Since both the scores are examples of ordinal data, Kappa statistics were calculated to assess the concordance between the two methods. In the previously quoted study by Tyagi, the agreement between ETCO 2 and PaCO 2 (both numerical variables) was represented using the Bland–Altman method. 8

Tests for assessing agreement between measurements

T ests for T ime-to -E vent D ata (S urvival A nalysis )

Time-to-event data represent a unique type of data where some participants have not experienced the outcome of interest at the time of analysis. Such participants are considered to be “censored” but are allowed to contribute to the analysis for the period of their follow-up. A detailed discussion on the analysis of time-to-event data is beyond the scope of this article. For analyzing time-to-event data, we use survival analysis (with the Kaplan–Meier method) and compare groups using the log-rank test. The risk of experiencing the event is expressed as a hazard ratio. Cox proportional hazards regression model is used to identify risk factors that are significantly associated with the event.

Hasanzadeh evaluated the impact of zinc supplementation on the development of ventilator-associated pneumonia (VAP) in adult mechanically ventilated trauma patients. 12 Survival analysis (Kaplan–Meier technique) was used to calculate the median time to development of VAP after ICU admission. The Cox proportional hazards regression model was used to calculate hazard ratios to identify factors significantly associated with the development of VAP.

The choice of statistical test used to analyze research data depends on the study hypothesis, the type of data, the number of measurements, and whether the data are paired or unpaired. Reviews of articles published in medical specialties such as family medicine, cytopathology, and pain have found several errors related to the use of descriptive and inferential statistics. 12 – 15 The statistical technique needs to be carefully chosen and specified in the protocol prior to commencement of the study, to ensure that the conclusions of the study are valid. This article has outlined the principles for selecting a statistical test, along with a list of tests used commonly. Researchers should seek help from statisticians while writing the research study protocol, to formulate the plan for statistical analysis.

Priya Ranganathan https://orcid.org/0000-0003-1004-5264

Source of support: Nil

Conflict of interest: None

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What do senior theses in Statistics look like?

This is a brief overview of thesis writing; for more information, please see our  complete guide here . Senior theses in Statistics cover a wide range of topics, across the spectrum from applied to theoretical. Typically, senior theses are expected to have one of the following three flavors:                                                                                                            

1. Novel statistical theory or methodology, supported by extensive mathematical and/or simulation results, along with a clear account of how the research extends or relates to previous related work.

2. An analysis of a complex data set that advances understanding in a related field, such as public health, economics, government, or genetics. Such a thesis may rely entirely on existing methods, but should give useful results and insights into an interesting applied problem.                                                                                 

3. An analysis of a complex data set in which new methods or modifications of published methods are required. While the thesis does not necessarily contain an extensive mathematical study of the new methods, it should contain strong plausibility arguments or simulations supporting the use of the new methods.

A good thesis is clear, readable, and well-motivated, justifying the applicability of the methods used rather than, for example, mechanically running regressions without discussing the assumptions (and whether they are plausible), performing diagnostics, and checking whether the conclusions make sense. 

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Top 9 Statistical Tools Used in Research

Well-designed research requires a well-chosen study sample and a suitable statistical test selection . To plan an epidemiological study or a clinical trial, you’ll need a solid understanding of the data . Improper inferences from it could lead to false conclusions and  unethical behavior . And given the ocean of data available nowadays, it’s often a daunting task for researchers to gauge its credibility and do statistical analysis on it.

With that said, thanks to all the statistical tools available in the market that help researchers make such studies much more manageable.  Statistical tools are   extensively used in academic and research sectors  to study human, animal, and material behaviors and reactions.

Statistical tools  aid in the interpretation and use of data. They can be used to evaluate and comprehend any form of data. Some statistical tools can help you see trends, forecast future sales, and create links between causes and effects. When you’re unsure where to go with your study, other tools can assist you in navigating through enormous amounts of data.

In this article, we will  discuss some  of the best statistical tools and their key features . So, let’s start without any further ado.

What is Statistics? And its Importance in Research

Statistics is the study of collecting, arranging, and interpreting data from samples and inferring it to the total population.  Also  known  as the “Science of Data,” it allows us to derive conclusions from a data set. It may also assist people in all industries in answering research or business queries and forecast outcomes, such as what show you should watch next on your favorite video app.

Statistical Tools Used in Research

Researchers often cannot discern a simple truth from a set of data. They can only draw conclusions from data after statistical analysis. On the other hand, creating a statistical analysis is a difficult task. This is when statistical tools come into play. Researchers can use statistical tools to back up their claims, make sense of a vast set of data, graphically show complex data, or help clarify many things in a short period. 

Let’s go through  the top 9 best statistical tools used in research  below:

SPSS first stores and organizes the data, then compile the data set to generate appropriate output. SPSS is intended to work with a wide range of variable data formats.

R  is a statistical computing and graphics programming language that you may use to clean, analyze and graph your data. It is frequently used to estimate and display results by researchers from various fields and lecturers of statistics and research methodologies. It’s free, making it an appealing option, but it relies upon programming code rather than drop-down menus or buttons. 

Many big tech companies are using SAS due to its support and integration for vast teams. Setting up the tool might be a bit time-consuming initially, but once it’s up and running, it’ll surely streamline your statistical processes.

Moreover, MATLAB provides a multi-paradigm numerical computing environment, which means that the language may be used for both procedural and object-oriented programming. MATLAB is ideal for matrix manipulation, including data function plotting, algorithm implementation, and user interface design, among other things. Last but not least, MATLAB can also  run programs  written in other programming languages. 

Some of the  highlights of Tableau  are:

7. MS EXCEL:

You can apply various formulas and functions to your data in Excel without prior knowledge of statistics. The learning curve is great, and even freshers can achieve great results quickly since everything is just a click away. This makes Excel a great choice not only for amateurs but beginners as well.

8. RAPIDMINER:

RapidMiner  is a valuable platform for data preparation, machine learning, and the deployment of predictive models. RapidMiner makes it simple to develop a data model from the beginning to the end. It comes with a complete data science suite. Machine learning, deep learning, text mining, and predictive analytics are all possible with it.

9. APACHE HADOOP:

Apache Hadoop  is an open-source software that is best known for its top-of-the-drawer scaling capabilities. It is capable of resolving the most challenging computational issues and excels at data-intensive activities as well, given its  distributed architecture . The primary reason why it outperforms its contenders in terms of computational power and speed is that it does not directly transfer files to the node. It divides enormous files into smaller bits and transmits them to separate nodes with specific instructions using  HDFS . More about it  here .

So, if you have massive data on your hands and want something that doesn’t slow you down and works in a distributed way, Hadoop is the way to go.

Some of the  highlights of Apache Hadoop  are:

Learn more about Statistics and Key Tools

Elasticity of Demand Explained in Plain Terms

An introduction to statistical power and a/b testing.

Statistical power is an integral part of A/B testing. And in this article, you will learn everything you need to know about it and how it is applied in A/B testing. A/B

What Data Analytics Tools Are And How To Use Them

Learn More…

There are a variety of software tools available, each of which offers something slightly different to the user – which one you choose will be determined by several things, including your research question, statistical understanding, and coding experience. These factors may indicate that you are on the cutting edge of data analysis, but the quality of the data acquired depends on the study execution, as with any research.

As an IT Engineer, who is passionate about learning and sharing. I have worked and learned quite a bit from Data Engineers, Data Analysts, Business Analysts, and Key Decision Makers almost for the past 5 years. Interested in learning more about Data Science and How to leverage it for better decision-making in my business and hopefully help you do the same in yours.

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2024 Theses Doctoral

Statistically Efficient Methods for Computation-Aware Uncertainty Quantification and Rare-Event Optimization

He, Shengyi

The thesis covers two fundamental topics that are important across the disciplines of operations research, statistics and even more broadly, namely stochastic optimization and uncertainty quantification, with the common theme to address both statistical accuracy and computational constraints. Here, statistical accuracy encompasses the precision of estimated solutions in stochastic optimization, as well as the tightness or reliability of confidence intervals. Computational concerns arise from rare events or expensive models, necessitating efficient sampling methods or computation procedures. In the first half of this thesis, we study stochastic optimization that involves rare events, which arises in various contexts including risk-averse decision-making and training of machine learning models. Because of the presence of rare events, crude Monte Carlo methods can be prohibitively inefficient, as it takes a sample size reciprocal to the rare-event probability to obtain valid statistical information about the rare-event. To address this issue, we investigate the use of importance sampling (IS) to reduce the required sample size. IS is commonly used to handle rare events, and the idea is to sample from an alternative distribution that hits the rare event more frequently and adjusts the estimator with a likelihood ratio to retain unbiasedness. While IS has been long studied, most of its literature focuses on estimation problems and methodologies to obtain good IS in these contexts. Contrary to these studies, the first half of this thesis provides a systematic study on the efficient use of IS in stochastic optimization. In Chapter 2, we propose an adaptive procedure that converts an efficient IS for gradient estimation to an efficient IS procedure for stochastic optimization. Then, in Chapter 3, we provide an efficient IS for gradient estimation, which serves as the input for the procedure in Chapter 2. In the second half of this thesis, we study uncertainty quantification in the sense of constructing a confidence interval (CI) for target model quantities or prediction. We are interested in the setting of expensive black-box models, which means that we are confined to using a low number of model runs, and we also lack the ability to obtain auxiliary model information such as gradients. In this case, a classical method is batching, which divides data into a few batches and then constructs a CI based on the batched estimates. Another method is the recently proposed cheap bootstrap that is constructed on a few resamples in a similar manner as batching. These methods could save computation since they do not need an accurate variability estimator which requires sufficient model evaluations to obtain. Instead, they cancel out the variability when constructing pivotal statistics, and thus obtain asymptotically valid t-distribution-based CIs with only few batches or resamples. The second half of this thesis studies several theoretical aspects of these computation-aware CI construction methods. In Chapter 4, we study the statistical optimality on CI tightness among various computation-aware CIs. Then, in Chapter 5, we study the higher-order coverage errors of batching methods. Finally, Chapter 6 is a related investigation on the higher-order coverage and correction of distributionally robust optimization (DRO) as another CI construction tool, which assumes an amount of analytical information on the model but bears similarity to Chapter 5 in terms of analysis techniques.

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• Stochastic processes--Mathematical models
• Mathematical optimization
• Bootstrap (Statistics)
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Standard statistical tools in research and data analysis

Introduction.

Statistics is a field of science concerned with gathering, organising, analysing, and extrapolating data from samples to the entire population. This necessitates a well-designed study, a well-chosen study sample, and a proper statistical test selection. A good understanding of statistics is required to design epidemiological research or a clinical trial. Improper statistical approaches might lead to erroneous findings and unethical behaviour.

A variable is a trait that differs from one person to the next within a population. Quantitative variables are measured by a scale and provide quantitative information, such as height and weight. Qualitative factors, such as sex and eye colour, provide qualitative information (Figure 1).

Figure 1. Classification of variables [1]

Quantitative variables

Discrete and continuous measures are used to split quantitative or numerical data. Continuous data can take on any value, whereas discrete numerical data is stored as a whole number such as 0, 1, 2, 3,… (integer). Discrete data is made up of countable observations, while continuous data is made up of measurable observations. Discrete data examples include the number of respiratory arrest episodes or re-intubation in an intensive care unit. Continuous data includes serial serum glucose levels, partial pressure of oxygen in arterial blood, and oesophageal temperature. A hierarchical scale with increasing precision can be used based on category, ordinal, interval and ratio scales (Figure 1).

Descriptive statistics try to explain how variables in a sample or population are related. The mean, median, and mode forms, descriptive statistics give an overview of data. Inferential statistics use a random sample of data from that group to characterise and infer about a community as a whole. It’s useful when it’s not possible to investigate every single person in a group.

Descriptive statistics

The central tendency describes how observations cluster about a centre point, whereas the degree of dispersion describes the spread towards the extremes.

Inferential statistics

In inferential statistics, data from a sample is analysed to conclude the entire population. The goal is to prove or disprove the theories. A hypothesis is a suggested explanation for a phenomenon (plural hypotheses). Hypothesis testing is essential to process for making logical choices regarding observed effects’ veracity.

SOFTWARES FOR STATISTICS, SAMPLE SIZE CALCULATION AND POWER ANALYSIS

There are several statistical software packages accessible today. The most commonly used software systems are Statistical Package for the Social Sciences (SPSS – manufactured by IBM corporation), Statistical Analysis System (SAS – developed by SAS Institute North Carolina, Minitab (developed by Minitab Inc), United States of America), R (designed by Ross Ihaka and Robert Gentleman from the R core team), Stata (developed by StataCorp), and MS Excel. There are several websites linked to statistical power studies. Here are a few examples:

• StatPages.net – contains connections to a variety of online power calculators.
• G-Power — a downloadable power analysis software that works on DOS.
• ANOVA power analysis creates an interactive webpage that estimates the power or sample size required to achieve a specified power for one effect in a factorial ANOVA design.
• Sample Power is software created by SPSS. It generates a comprehensive report on the computer screen that may be copied and pasted into another document.

A researcher must be familiar with the most important statistical approaches for doing research. This will aid in the implementation of a well-designed study that yields accurate and valid data. Incorrect statistical approaches can result in erroneous findings, mistakes, and reduced paper’s importance. Poor statistics can lead to poor research, which can lead to immoral behaviour. As a result, proper statistical understanding and the right application of statistical tests are essential. A thorough understanding of fundamental statistical methods will go a long way toward enhancing study designs and creating high-quality medical research that may be used to develop evidence-based guidelines.

[1] Ali, Zulfiqar, and S Bala Bhaskar. “Basic statistical tools in research and data analysis.”  Indian journal of anaesthesia  vol. 60,9 (2016): 662-669. doi:10.4103/0019-5049.190623

[2] Ali, Zulfiqar, and S Bala Bhaskar. “Basic statistical tools in research and data analysis.” Indian journal of anaesthesia vol. 60,9 (2016): 662-669. doi:10.4103/0019-5049.190623

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Statistical Treatment

Statistics Definitions > Statistical Treatment

What is Statistical Treatment?

Statistical treatment can mean a few different things:

• In Data Analysis : Applying any statistical method — like regression or calculating a mean — to data.
• In Factor Analysis : Any combination of factor levels is called a treatment.
• In a Thesis or Experiment : A summary of the procedure, including statistical methods used.

1. Statistical Treatment in Data Analysis

The term “statistical treatment” is a catch all term which means to apply any statistical method to your data. Treatments are divided into two groups: descriptive statistics , which summarize your data as a graph or summary statistic and inferential statistics , which make predictions and test hypotheses about your data. Treatments could include:

• Finding standard deviations and sample standard errors ,
• Finding T-Scores or Z-Scores .
• Calculating Correlation coefficients .

2. Treatments in Factor Analysis

3. Treatments in a Thesis or Experiment

Sometimes you might be asked to include a treatment as part of a thesis. This is asking you to summarize the data and analysis portion of your experiment, including measurements and formulas used. For example, the following experimental summary is from Statistical Treatment in Acta Physiologica Scandinavica. :

Each of the test solutions was injected twice in each subject…30-42 values were obtained for the intensity, and a like number for the duration, of the pain indiced by the solution. The pain values reported in the following are arithmetical means for these 30-42 injections.”

The author goes on to provide formulas for the mean, the standard deviation and the standard error of the mean.

Vogt, W.P. (2005). Dictionary of Statistics & Methodology: A Nontechnical Guide for the Social Sciences . SAGE. Wheelan, C. (2014). Naked Statistics . W. W. Norton & Company Unknown author (1961) Chapter 3: Statistical Treatment. Acta Physiologica Scandinavica. Volume 51, Issue s179 December Pages 16–20.

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Statsomat is a web platform that aims to provide automated guidance and apps for automated statistical analysis of data, specifically designed for adult learners of data analysis and data literacy, who are often students and young researchers. Statsomat aims to simulate unavailable academic consultancy for statistical data analysis. Statsomat supports data literacy education, free of charge.

Statsomat is a project in continuous progress. The core contributor authoring the content of the apps is Dr. Denise Welsch . Other contributors are students of the University of Applied Sciences Koblenz , Department of Mathematics and Technology.

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Table of Contents

In research methodology, statistical tools form the foundation for analysing data, drawing results, and making informed choices. In academic, business, or scientific areas, knowing about different types of statistical tools is crucial to carrying out thorough and significant research. This thorough guide explores the main statistical tools used in research methodology . We focus on how they apply to quantitative research across different areas.

Research Design and Methodology

Rather than starting with specific statistical tools, we must first comprehend the general context of research design and methodology . Research design means the complete plan or strategy selected to incorporate various parts of the study together in an organised and rational manner. The methodology comprises different methods, techniques, procedures, and data collection and analysis tools. Research design and methodology work in tandem to create a structure for systematic investigation.

Quantitative Research Methodology

Quantitative research methodology gathers and studies numerical data to find patterns, connections, and tendencies. Statistical tools are essential in quantitative research because they help researchers measure phenomena, check theories, and make generalisations from sample data for a bigger population set. Certain basic statistical tools employed in quantitative research methodology are as follows:

 1. Descriptive Statistics

Descriptive statistics give a summary and description of the main aspects of a dataset. This includes features like central tendency (mean, median, or mode) and dispersion measures such as standard deviation and range, among others. They help understand the characteristics and distribution of the data.

2. Inferential Statistics

Inferential statistics are methods researchers use to draw conclusions or predictions about a whole group, known as the population, from the information collected in samples. The usual inferential activities include hypothesis testing, confidence intervals, and regression analysis, which help study connections among variables and measure how vital specific findings are.

3. Sampling Methods

Sampling techniques, such as simple random, stratified, or cluster sampling, help ensure that the selected sample represents the population well. This improves the generalizability of research results and allows researchers to make broader inferences about their findings.

4. Correlation and Regression Analysis

In correlation analysis, we investigate the power and direction of connection between two or more variables. This gives us an understanding of patterns in the association. Regression analysis is different because it investigates a predictive relationship between the independent and dependent variables. This allows researchers to create predictive models and recognise what factors are influential.

Business Research Methodology

Within business research methodology, statistical tools are vital in examining market patterns, customer actions, and organisations’ performance. When conducting studies on the market, like surveys or analysing financial aspects along with operational research, they help to gather valuable insights that aid businesses in making thoughtful and strategic decisions. Some critical statistical tools that are utilised in business research methodology involve:

1.Market Research Surveys

Frequency distributions, cross-tabulations, chi-square tests, and more are used in survey design and analysis to analyse survey responses. These tools help examine the answers, find market trends, and understand what customers like.

2.Predictive Analytics

Time series analysis, decision trees, and logistic regression are all predictive analytics models that businesses use. They help predict future results, find market opportunities, and reduce risks.

Statistical tools are necessary for rigorous research across all fields. Knowing the different types of statistical techniques is essential in academic, business, or science investigations to analyse data well and make wise decisions. UpGrad’s ‘ Introduction to Research Methodology’ course can be the stepping stone for your career. When we learn how to use statistical tools correctly and include them in our research design and process, we can improve the quality of our findings.

1. Why are statistical tools important in research methodology?

They are significant in research methodology because they help to analyse data and reach dependable outcomes. They guarantee that research discoveries are precise and trustworthy.

2. What is the difference between descriptive and inferential statistics?

Descriptive statistics summarise and explain the main characteristics of a dataset; on the other hand, inferential statistics are used to make predictions or inferences about a population from sample data.

3. How do I choose the right statistical tool for my research?

The selection of a statistical tool depends on your research question, the data you possess, and the exact analysis needed. You can ask a statistician or utilise statistical software to determine which tool is suitable.

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The Beginner's Guide to Statistical Analysis | 5 Steps & Examples

Statistical analysis means investigating trends, patterns, and relationships using quantitative data . It is an important research tool used by scientists, governments, businesses, and other organisations.

To draw valid conclusions, statistical analysis requires careful planning from the very start of the research process . You need to specify your hypotheses and make decisions about your research design, sample size, and sampling procedure.

After collecting data from your sample, you can organise and summarise the data using descriptive statistics . Then, you can use inferential statistics to formally test hypotheses and make estimates about the population. Finally, you can interpret and generalise your findings.

This article is a practical introduction to statistical analysis for students and researchers. We’ll walk you through the steps using two research examples. The first investigates a potential cause-and-effect relationship, while the second investigates a potential correlation between variables.

Table of contents

Step 1: write your hypotheses and plan your research design, step 2: collect data from a sample, step 3: summarise your data with descriptive statistics, step 4: test hypotheses or make estimates with inferential statistics, step 5: interpret your results, frequently asked questions about statistics.

To collect valid data for statistical analysis, you first need to specify your hypotheses and plan out your research design.

Writing statistical hypotheses

The goal of research is often to investigate a relationship between variables within a population . You start with a prediction, and use statistical analysis to test that prediction.

A statistical hypothesis is a formal way of writing a prediction about a population. Every research prediction is rephrased into null and alternative hypotheses that can be tested using sample data.

While the null hypothesis always predicts no effect or no relationship between variables, the alternative hypothesis states your research prediction of an effect or relationship.

• Null hypothesis: A 5-minute meditation exercise will have no effect on math test scores in teenagers.
• Alternative hypothesis: A 5-minute meditation exercise will improve math test scores in teenagers.
• Null hypothesis: Parental income and GPA have no relationship with each other in college students.
• Alternative hypothesis: Parental income and GPA are positively correlated in college students.

Planning your research design

A research design is your overall strategy for data collection and analysis. It determines the statistical tests you can use to test your hypothesis later on.

First, decide whether your research will use a descriptive, correlational, or experimental design. Experiments directly influence variables, whereas descriptive and correlational studies only measure variables.

• In an experimental design , you can assess a cause-and-effect relationship (e.g., the effect of meditation on test scores) using statistical tests of comparison or regression.
• In a correlational design , you can explore relationships between variables (e.g., parental income and GPA) without any assumption of causality using correlation coefficients and significance tests.
• In a descriptive design , you can study the characteristics of a population or phenomenon (e.g., the prevalence of anxiety in U.S. college students) using statistical tests to draw inferences from sample data.

Your research design also concerns whether you’ll compare participants at the group level or individual level, or both.

• In a between-subjects design , you compare the group-level outcomes of participants who have been exposed to different treatments (e.g., those who performed a meditation exercise vs those who didn’t).
• In a within-subjects design , you compare repeated measures from participants who have participated in all treatments of a study (e.g., scores from before and after performing a meditation exercise).
• In a mixed (factorial) design , one variable is altered between subjects and another is altered within subjects (e.g., pretest and posttest scores from participants who either did or didn’t do a meditation exercise).
• Experimental
• Correlational

First, you’ll take baseline test scores from participants. Then, your participants will undergo a 5-minute meditation exercise. Finally, you’ll record participants’ scores from a second math test.

In this experiment, the independent variable is the 5-minute meditation exercise, and the dependent variable is the math test score from before and after the intervention. Example: Correlational research design In a correlational study, you test whether there is a relationship between parental income and GPA in graduating college students. To collect your data, you will ask participants to fill in a survey and self-report their parents’ incomes and their own GPA.

Measuring variables

When planning a research design, you should operationalise your variables and decide exactly how you will measure them.

For statistical analysis, it’s important to consider the level of measurement of your variables, which tells you what kind of data they contain:

• Categorical data represents groupings. These may be nominal (e.g., gender) or ordinal (e.g. level of language ability).
• Quantitative data represents amounts. These may be on an interval scale (e.g. test score) or a ratio scale (e.g. age).

Many variables can be measured at different levels of precision. For example, age data can be quantitative (8 years old) or categorical (young). If a variable is coded numerically (e.g., level of agreement from 1–5), it doesn’t automatically mean that it’s quantitative instead of categorical.

Identifying the measurement level is important for choosing appropriate statistics and hypothesis tests. For example, you can calculate a mean score with quantitative data, but not with categorical data.

In a research study, along with measures of your variables of interest, you’ll often collect data on relevant participant characteristics.

In most cases, it’s too difficult or expensive to collect data from every member of the population you’re interested in studying. Instead, you’ll collect data from a sample.

Statistical analysis allows you to apply your findings beyond your own sample as long as you use appropriate sampling procedures . You should aim for a sample that is representative of the population.

Sampling for statistical analysis

There are two main approaches to selecting a sample.

• Probability sampling: every member of the population has a chance of being selected for the study through random selection.
• Non-probability sampling: some members of the population are more likely than others to be selected for the study because of criteria such as convenience or voluntary self-selection.

In theory, for highly generalisable findings, you should use a probability sampling method. Random selection reduces sampling bias and ensures that data from your sample is actually typical of the population. Parametric tests can be used to make strong statistical inferences when data are collected using probability sampling.

But in practice, it’s rarely possible to gather the ideal sample. While non-probability samples are more likely to be biased, they are much easier to recruit and collect data from. Non-parametric tests are more appropriate for non-probability samples, but they result in weaker inferences about the population.

If you want to use parametric tests for non-probability samples, you have to make the case that:

• your sample is representative of the population you’re generalising your findings to.
• your sample lacks systematic bias.

Keep in mind that external validity means that you can only generalise your conclusions to others who share the characteristics of your sample. For instance, results from Western, Educated, Industrialised, Rich and Democratic samples (e.g., college students in the US) aren’t automatically applicable to all non-WEIRD populations.

If you apply parametric tests to data from non-probability samples, be sure to elaborate on the limitations of how far your results can be generalised in your discussion section .

Create an appropriate sampling procedure

Based on the resources available for your research, decide on how you’ll recruit participants.

• Will you have resources to advertise your study widely, including outside of your university setting?
• Will you have the means to recruit a diverse sample that represents a broad population?
• Do you have time to contact and follow up with members of hard-to-reach groups?

Your participants are self-selected by their schools. Although you’re using a non-probability sample, you aim for a diverse and representative sample. Example: Sampling (correlational study) Your main population of interest is male college students in the US. Using social media advertising, you recruit senior-year male college students from a smaller subpopulation: seven universities in the Boston area.

Calculate sufficient sample size

Before recruiting participants, decide on your sample size either by looking at other studies in your field or using statistics. A sample that’s too small may be unrepresentative of the sample, while a sample that’s too large will be more costly than necessary.

There are many sample size calculators online. Different formulas are used depending on whether you have subgroups or how rigorous your study should be (e.g., in clinical research). As a rule of thumb, a minimum of 30 units or more per subgroup is necessary.

To use these calculators, you have to understand and input these key components:

• Significance level (alpha): the risk of rejecting a true null hypothesis that you are willing to take, usually set at 5%.
• Statistical power : the probability of your study detecting an effect of a certain size if there is one, usually 80% or higher.
• Expected effect size : a standardised indication of how large the expected result of your study will be, usually based on other similar studies.
• Population standard deviation: an estimate of the population parameter based on a previous study or a pilot study of your own.

Once you’ve collected all of your data, you can inspect them and calculate descriptive statistics that summarise them.

Inspect your data

There are various ways to inspect your data, including the following:

• Organising data from each variable in frequency distribution tables .
• Displaying data from a key variable in a bar chart to view the distribution of responses.
• Visualising the relationship between two variables using a scatter plot .

By visualising your data in tables and graphs, you can assess whether your data follow a skewed or normal distribution and whether there are any outliers or missing data.

A normal distribution means that your data are symmetrically distributed around a center where most values lie, with the values tapering off at the tail ends.

In contrast, a skewed distribution is asymmetric and has more values on one end than the other. The shape of the distribution is important to keep in mind because only some descriptive statistics should be used with skewed distributions.

Extreme outliers can also produce misleading statistics, so you may need a systematic approach to dealing with these values.

Calculate measures of central tendency

Measures of central tendency describe where most of the values in a data set lie. Three main measures of central tendency are often reported:

• Mode : the most popular response or value in the data set.
• Median : the value in the exact middle of the data set when ordered from low to high.
• Mean : the sum of all values divided by the number of values.

However, depending on the shape of the distribution and level of measurement, only one or two of these measures may be appropriate. For example, many demographic characteristics can only be described using the mode or proportions, while a variable like reaction time may not have a mode at all.

Calculate measures of variability

Measures of variability tell you how spread out the values in a data set are. Four main measures of variability are often reported:

• Range : the highest value minus the lowest value of the data set.
• Interquartile range : the range of the middle half of the data set.
• Standard deviation : the average distance between each value in your data set and the mean.
• Variance : the square of the standard deviation.

Once again, the shape of the distribution and level of measurement should guide your choice of variability statistics. The interquartile range is the best measure for skewed distributions, while standard deviation and variance provide the best information for normal distributions.

Using your table, you should check whether the units of the descriptive statistics are comparable for pretest and posttest scores. For example, are the variance levels similar across the groups? Are there any extreme values? If there are, you may need to identify and remove extreme outliers in your data set or transform your data before performing a statistical test.

From this table, we can see that the mean score increased after the meditation exercise, and the variances of the two scores are comparable. Next, we can perform a statistical test to find out if this improvement in test scores is statistically significant in the population. Example: Descriptive statistics (correlational study) After collecting data from 653 students, you tabulate descriptive statistics for annual parental income and GPA.

It’s important to check whether you have a broad range of data points. If you don’t, your data may be skewed towards some groups more than others (e.g., high academic achievers), and only limited inferences can be made about a relationship.

A number that describes a sample is called a statistic , while a number describing a population is called a parameter . Using inferential statistics , you can make conclusions about population parameters based on sample statistics.

Researchers often use two main methods (simultaneously) to make inferences in statistics.

• Estimation: calculating population parameters based on sample statistics.
• Hypothesis testing: a formal process for testing research predictions about the population using samples.

You can make two types of estimates of population parameters from sample statistics:

• A point estimate : a value that represents your best guess of the exact parameter.
• An interval estimate : a range of values that represent your best guess of where the parameter lies.

If your aim is to infer and report population characteristics from sample data, it’s best to use both point and interval estimates in your paper.

You can consider a sample statistic a point estimate for the population parameter when you have a representative sample (e.g., in a wide public opinion poll, the proportion of a sample that supports the current government is taken as the population proportion of government supporters).

There’s always error involved in estimation, so you should also provide a confidence interval as an interval estimate to show the variability around a point estimate.

A confidence interval uses the standard error and the z score from the standard normal distribution to convey where you’d generally expect to find the population parameter most of the time.

Hypothesis testing

Using data from a sample, you can test hypotheses about relationships between variables in the population. Hypothesis testing starts with the assumption that the null hypothesis is true in the population, and you use statistical tests to assess whether the null hypothesis can be rejected or not.

Statistical tests determine where your sample data would lie on an expected distribution of sample data if the null hypothesis were true. These tests give two main outputs:

• A test statistic tells you how much your data differs from the null hypothesis of the test.
• A p value tells you the likelihood of obtaining your results if the null hypothesis is actually true in the population.

Statistical tests come in three main varieties:

• Comparison tests assess group differences in outcomes.
• Regression tests assess cause-and-effect relationships between variables.
• Correlation tests assess relationships between variables without assuming causation.

Your choice of statistical test depends on your research questions, research design, sampling method, and data characteristics.

Parametric tests

Parametric tests make powerful inferences about the population based on sample data. But to use them, some assumptions must be met, and only some types of variables can be used. If your data violate these assumptions, you can perform appropriate data transformations or use alternative non-parametric tests instead.

A regression models the extent to which changes in a predictor variable results in changes in outcome variable(s).

• A simple linear regression includes one predictor variable and one outcome variable.
• A multiple linear regression includes two or more predictor variables and one outcome variable.

Comparison tests usually compare the means of groups. These may be the means of different groups within a sample (e.g., a treatment and control group), the means of one sample group taken at different times (e.g., pretest and posttest scores), or a sample mean and a population mean.

• A t test is for exactly 1 or 2 groups when the sample is small (30 or less).
• A z test is for exactly 1 or 2 groups when the sample is large.
• An ANOVA is for 3 or more groups.

The z and t tests have subtypes based on the number and types of samples and the hypotheses:

• If you have only one sample that you want to compare to a population mean, use a one-sample test .
• If you have paired measurements (within-subjects design), use a dependent (paired) samples test .
• If you have completely separate measurements from two unmatched groups (between-subjects design), use an independent (unpaired) samples test .
• If you expect a difference between groups in a specific direction, use a one-tailed test .
• If you don’t have any expectations for the direction of a difference between groups, use a two-tailed test .

The only parametric correlation test is Pearson’s r . The correlation coefficient ( r ) tells you the strength of a linear relationship between two quantitative variables.

However, to test whether the correlation in the sample is strong enough to be important in the population, you also need to perform a significance test of the correlation coefficient, usually a t test, to obtain a p value. This test uses your sample size to calculate how much the correlation coefficient differs from zero in the population.

You use a dependent-samples, one-tailed t test to assess whether the meditation exercise significantly improved math test scores. The test gives you:

• a t value (test statistic) of 3.00
• a p value of 0.0028

Although Pearson’s r is a test statistic, it doesn’t tell you anything about how significant the correlation is in the population. You also need to test whether this sample correlation coefficient is large enough to demonstrate a correlation in the population.

A t test can also determine how significantly a correlation coefficient differs from zero based on sample size. Since you expect a positive correlation between parental income and GPA, you use a one-sample, one-tailed t test. The t test gives you:

• a t value of 3.08
• a p value of 0.001

The final step of statistical analysis is interpreting your results.

Statistical significance

In hypothesis testing, statistical significance is the main criterion for forming conclusions. You compare your p value to a set significance level (usually 0.05) to decide whether your results are statistically significant or non-significant.

Statistically significant results are considered unlikely to have arisen solely due to chance. There is only a very low chance of such a result occurring if the null hypothesis is true in the population.

This means that you believe the meditation intervention, rather than random factors, directly caused the increase in test scores. Example: Interpret your results (correlational study) You compare your p value of 0.001 to your significance threshold of 0.05. With a p value under this threshold, you can reject the null hypothesis. This indicates a statistically significant correlation between parental income and GPA in male college students.

Note that correlation doesn’t always mean causation, because there are often many underlying factors contributing to a complex variable like GPA. Even if one variable is related to another, this may be because of a third variable influencing both of them, or indirect links between the two variables.

Effect size

A statistically significant result doesn’t necessarily mean that there are important real life applications or clinical outcomes for a finding.

In contrast, the effect size indicates the practical significance of your results. It’s important to report effect sizes along with your inferential statistics for a complete picture of your results. You should also report interval estimates of effect sizes if you’re writing an APA style paper .

With a Cohen’s d of 0.72, there’s medium to high practical significance to your finding that the meditation exercise improved test scores. Example: Effect size (correlational study) To determine the effect size of the correlation coefficient, you compare your Pearson’s r value to Cohen’s effect size criteria.

Decision errors

Type I and Type II errors are mistakes made in research conclusions. A Type I error means rejecting the null hypothesis when it’s actually true, while a Type II error means failing to reject the null hypothesis when it’s false.

You can aim to minimise the risk of these errors by selecting an optimal significance level and ensuring high power . However, there’s a trade-off between the two errors, so a fine balance is necessary.

Frequentist versus Bayesian statistics

Traditionally, frequentist statistics emphasises null hypothesis significance testing and always starts with the assumption of a true null hypothesis.

However, Bayesian statistics has grown in popularity as an alternative approach in the last few decades. In this approach, you use previous research to continually update your hypotheses based on your expectations and observations.

Bayes factor compares the relative strength of evidence for the null versus the alternative hypothesis rather than making a conclusion about rejecting the null hypothesis or not.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

The research methods you use depend on the type of data you need to answer your research question .

• If you want to measure something or test a hypothesis , use quantitative methods . If you want to explore ideas, thoughts, and meanings, use qualitative methods .
• If you want to analyse a large amount of readily available data, use secondary data. If you want data specific to your purposes with control over how they are generated, collect primary data.
• If you want to establish cause-and-effect relationships between variables , use experimental methods. If you want to understand the characteristics of a research subject, use descriptive methods.

Statistical analysis is the main method for analyzing quantitative research data . It uses probabilities and models to test predictions about a population from sample data.

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Statistics Say the Median Retirement Savings for American Households Is $87,000. How to Help Grow That Number to a Million

• Saving from a young age could help you grow a lot of retirement wealth.
• It's also important to invest your savings wisely.
• Motley Fool Issues Rare “All In” Buy Alert

Why settle for a tiny nest egg when you could have a huge one?

The average retiree on Social Security today gets about $1,915 a month, or roughly $23,000 a year. If the idea of having to live on such little income scares you, then the solution is simple: You'll need to build up a retirement nest egg so you have money outside of Social Security to fall back on.

Motley Fool research finds that the average retirement savings balance is $333,945 based on the most recent data available. But the median retirement plan balance is only $87,000. And when you have a median that's considerably lower than the average, it's an indication that the median is a more representative figure.

Image source: Getty Images.

A retirement savings balance of $87,000 is clearly better than a $0 balance. And it's also important to note that $87,000 in retirement savings means something very different for a 35-year-old than for a 62-year-old.

But let's be real -- you probably want to do a lot better than $87,000 in the context of growing your retirement nest egg. The good news is that if you play your cards right, you could wind up with $1 million to your name -- and a really sweet retirement to follow.

It's all about starting young and choosing the right investments

You might assume that to retire with $1 million, you need to give up every possible enjoyable thing you spend money on and save every cent of your paycheck you can. Well, that's just not true. You can actually get away with saving very little of your income and still wind up with $1 million. But to do that, you need two things:

• The right investment strategy.

The first simply means you need to start saving for retirement early on. Don't wait until your 40s even though it may be tough to carve out money for retirement savings when you're younger. The more years you have to enjoy compounded returns in your 401(k) or IRA, the larger a balance you stand to wind up with.

For the second, the right investment strategy should really focus on stocks. While they carry risk, they've historically offered a lot of reward.

If you're not confident in your ability to put together a portfolio of individual stocks, though, fear not. You can do quite well for yourself by loading up on S&P 500 index funds .

The path to $1 million may be pretty smooth

So let's put everything together, shall we? Imagine you're 26 years old and are ready to get serious about retirement savings.

If you sock away $300 a month in a retirement account between now and age 67, which is your full retirement age for Social Security purposes, and your portfolio generates an average annual 8% return, which is a notch below the stock market's average, you could end up with just over $1 million.

Meanwhile, the Bureau of Labor Statistics puts the median annual wage for 26-year-olds today at about $56,000 . Contributing $3,600 of that toward retirement means parting with about 6.5% of your income. That's not an easy thing to do per se, but it's fairly reasonable. After all, it's not like you're looking at giving up 25% of your paycheck.

So there you have it. An $87,000 nest egg might make for a more comfortable retirement than a $0 nest egg. But if you really want to enjoy your senior years to the fullest, then it pays to aim much higher. And you may be surprised at how easy it is to amass a full million by the time your career is set to wrap up.

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THE NOISE AND INFLUENCE ON FLUORESCENCE MICROSCOPY

Fluorescence microscopy, a cornerstone in biological imaging, faces inherent challenges due to photon budget constraints that affect the signal-to-noise ratio (SNR), ultimately limiting imaging performance. This thesis explores theoretical frameworks to address two fundamental issues: the denoising limit of fluorescence microscopy images and the resolution limit in the presence of photon noise. Firstly, we extend the application of the Cramér-Rao Lower Bound (CRLB) to establish a variance lower bound for image denoising algorithms in fluorescence microscopy. By incorporating constraints specific to the imaging system and biological specimens, we provide a benchmark for evaluating the performance of state-of-the-art denoising algorithms. Our analysis reveals that this lower bound is determined by factors such as photon count, readout noise, detection wavelength, effective pixel size, and numerical aperture of the microscope system. Secondly, building upon the pioneering work by Ernest Abbe and leveraging modern fluorescence and nanoscopy advancements, we propose a novel theoretical framework to quantify the resolving power of fluorescence microscopes under finite photon conditions. This model integrates the traditional diffraction limit with photon statistics to determine the practical resolution limit, highlighting the trade-offs between photon noise and resolution enhancement in techniques like confocal microscopy. This dual approach not only refined the theoretical understanding of fluorescence microscopy's capabilities but also assisted in designing and optimizing more effective imaging protocols. Through these investigations, this thesis provided a comprehensive theoretical foundation for improving fluorescence microscopy imaging techniques, paving the way for future innovations in biological imaging.

Degree Type

• Doctor of Philosophy
• Biomedical Engineering

Campus location

• West Lafayette

Advisor/Supervisor/Committee Chair

Additional committee member 2, additional committee member 3, additional committee member 4, usage metrics.

• Biomedical imaging

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EV Life Cycle Assessment Calculator

Explore and compare the lifecycle emissions of conventional and electric cars and the effect of changing variables such as vehicle size

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Reach & Traffic

• Leading websites worldwide 2023, by monthly visits

Between September and November 2023, Google.com held the leading position as the most popular website worldwide with 175 billion average monthly visits. The online platform has held the top spot as the most popular website since June 2010, when it pulled ahead of Yahoo into first place. Second-ranked YouTube generated more than 113 billion monthly visits in the measured period.

The internet leaders: search, social, and e-commerce

What is next for online content, most popular websites worldwide as of november 2023, by total visits (in billions).

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Social Media & User-Generated Content

• Global social networks ranked by number of users 2024

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• Most linked to websites worldwide 2024
• Brazil: most visited websites 2023, by total visits
• China: leading websites 2024, by total visits
• India: leading websites 2023, by total visits
• Most visited websites in Japan 2022-2023, by average monthly traffic
• Page traffic of most popular websites in Russia 2023
• UK: leading websites 2023, by total visits
• U.S. most visited websites 2023, by total visits
• Total global visitor traffic to user-generated content websites 2023
• Total global visitor traffic to Facebook.com 2023
• Total global visitor traffic to Instagram.com 2023
• Total global visitor traffic to Reddit.com 2022-2024, by device
• Total global visitor traffic to Twitter.com 2023
• Total global visitor traffic to Wikipedia.org 2023
• Global market share of leading search engines 2015-2024
• Global market share of leading mobile search engines 2015-2024
• Total global visitor traffic to Google.com 2023
• Total global visitor traffic to Bing.com 2023
• Leading video streaming websites worldwide 2023, based on visit share
• Monthly global visitor traffic to YouTube.com 2023, by device
• Number of SVOD subscribers worldwide 2023, by country
• Number of SVOD subscribers worldwide 2027, by region and platform
• Leading e-commerce and shopping websites worldwide 2023, based on visit share
• YoY online traffic growth worldwide 2022-2023, by e-commerce sector
• Leading beauty and cosmetics websites worldwide 2022-2024, by monthly visits
• Leading food and beverage websites worldwide 2022-2024, by monthly visits
• Leading consumer electronics sites worldwide 2024, by monthly visits
• Number of page views per web session 2022, by vertical & device
• Global news site traffic growth 2024
• Online subscriptions to news websites worldwide 2023
• English-language news websites on TikTok worldwide 2023, by follower count
• Social network usage for news access worldwide 2019-2023
• Decline in aggregate social media traffic to news sites worldwide 2022-2023
• Leading news websites in the United Kingdom (UK) 2023, by monthly visits

Other statistics that may interest you Top websites worldwide

• Basic Statistic Leading websites worldwide 2023, by monthly visits
• Basic Statistic Leading websites worldwide 2023, by unique visitors
• Basic Statistic Leading websites worldwide 2023, by session length
• Basic Statistic Share of global mobile website traffic 2015-2023
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Regional leaders

• Basic Statistic Brazil: most visited websites 2023, by total visits
• Premium Statistic China: leading websites 2024, by total visits
• Premium Statistic India: leading websites 2023, by total visits
• Premium Statistic Most visited websites in Japan 2022-2023, by average monthly traffic
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• Basic Statistic U.S. most visited websites 2023, by total visits

Social platforms

• Basic Statistic Global social networks ranked by number of users 2024
• Basic Statistic Total global visitor traffic to user-generated content websites 2023
• Basic Statistic Total global visitor traffic to Facebook.com 2023
• Basic Statistic Total global visitor traffic to Instagram.com 2023
• Premium Statistic Total global visitor traffic to Reddit.com 2022-2024, by device
• Premium Statistic Total global visitor traffic to Twitter.com 2023
• Basic Statistic Total global visitor traffic to Wikipedia.org 2023
• Basic Statistic Global market share of leading search engines 2015-2024
• Basic Statistic Global market share of leading desktop search engines 2015-2024
• Basic Statistic Global market share of leading mobile search engines 2015-2024
• Basic Statistic Total global visitor traffic to Google.com 2023
• Basic Statistic Total global visitor traffic to Bing.com 2023

Video and streaming

• Basic Statistic Leading video streaming websites worldwide 2023, based on visit share
• Premium Statistic Monthly global visitor traffic to YouTube.com 2023, by device
• Premium Statistic Number of SVOD subscribers worldwide 2023, by country
• Premium Statistic Number of SVOD subscribers worldwide 2027, by region and platform
• Premium Statistic Leading e-commerce and shopping websites worldwide 2023, based on visit share
• Premium Statistic YoY online traffic growth worldwide 2022-2023, by e-commerce sector
• Premium Statistic Leading beauty and cosmetics websites worldwide 2022-2024, by monthly visits
• Premium Statistic Leading food and beverage websites worldwide 2022-2024, by monthly visits
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• Premium Statistic Number of page views per web session 2022, by vertical & device

Spotlight: online news

• Basic Statistic Global news site traffic growth 2024
• Premium Statistic Online subscriptions to news websites worldwide 2023
• Basic Statistic English-language news websites on TikTok worldwide 2023, by follower count
• Premium Statistic Social network usage for news access worldwide 2019-2023
• Basic Statistic Decline in aggregate social media traffic to news sites worldwide 2022-2023
• Premium Statistic Leading news websites in the United Kingdom (UK) 2023, by monthly visits

Further related statistics

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