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A Practical Guide to Writing Quantitative and Qualitative Research Questions and Hypotheses in Scholarly Articles

Edward barroga.

1 Department of General Education, Graduate School of Nursing Science, St. Luke’s International University, Tokyo, Japan.

Glafera Janet Matanguihan

2 Department of Biological Sciences, Messiah University, Mechanicsburg, PA, USA.

The development of research questions and the subsequent hypotheses are prerequisites to defining the main research purpose and specific objectives of a study. Consequently, these objectives determine the study design and research outcome. The development of research questions is a process based on knowledge of current trends, cutting-edge studies, and technological advances in the research field. Excellent research questions are focused and require a comprehensive literature search and in-depth understanding of the problem being investigated. Initially, research questions may be written as descriptive questions which could be developed into inferential questions. These questions must be specific and concise to provide a clear foundation for developing hypotheses. Hypotheses are more formal predictions about the research outcomes. These specify the possible results that may or may not be expected regarding the relationship between groups. Thus, research questions and hypotheses clarify the main purpose and specific objectives of the study, which in turn dictate the design of the study, its direction, and outcome. Studies developed from good research questions and hypotheses will have trustworthy outcomes with wide-ranging social and health implications.

INTRODUCTION

Scientific research is usually initiated by posing evidenced-based research questions which are then explicitly restated as hypotheses. 1 , 2 The hypotheses provide directions to guide the study, solutions, explanations, and expected results. 3 , 4 Both research questions and hypotheses are essentially formulated based on conventional theories and real-world processes, which allow the inception of novel studies and the ethical testing of ideas. 5 , 6

It is crucial to have knowledge of both quantitative and qualitative research 2 as both types of research involve writing research questions and hypotheses. 7 However, these crucial elements of research are sometimes overlooked; if not overlooked, then framed without the forethought and meticulous attention it needs. Planning and careful consideration are needed when developing quantitative or qualitative research, particularly when conceptualizing research questions and hypotheses. 4

There is a continuing need to support researchers in the creation of innovative research questions and hypotheses, as well as for journal articles that carefully review these elements. 1 When research questions and hypotheses are not carefully thought of, unethical studies and poor outcomes usually ensue. Carefully formulated research questions and hypotheses define well-founded objectives, which in turn determine the appropriate design, course, and outcome of the study. This article then aims to discuss in detail the various aspects of crafting research questions and hypotheses, with the goal of guiding researchers as they develop their own. Examples from the authors and peer-reviewed scientific articles in the healthcare field are provided to illustrate key points.

DEFINITIONS AND RELATIONSHIP OF RESEARCH QUESTIONS AND HYPOTHESES

A research question is what a study aims to answer after data analysis and interpretation. The answer is written in length in the discussion section of the paper. Thus, the research question gives a preview of the different parts and variables of the study meant to address the problem posed in the research question. 1 An excellent research question clarifies the research writing while facilitating understanding of the research topic, objective, scope, and limitations of the study. 5

On the other hand, a research hypothesis is an educated statement of an expected outcome. This statement is based on background research and current knowledge. 8 , 9 The research hypothesis makes a specific prediction about a new phenomenon 10 or a formal statement on the expected relationship between an independent variable and a dependent variable. 3 , 11 It provides a tentative answer to the research question to be tested or explored. 4

Hypotheses employ reasoning to predict a theory-based outcome. 10 These can also be developed from theories by focusing on components of theories that have not yet been observed. 10 The validity of hypotheses is often based on the testability of the prediction made in a reproducible experiment. 8

Conversely, hypotheses can also be rephrased as research questions. Several hypotheses based on existing theories and knowledge may be needed to answer a research question. Developing ethical research questions and hypotheses creates a research design that has logical relationships among variables. These relationships serve as a solid foundation for the conduct of the study. 4 , 11 Haphazardly constructed research questions can result in poorly formulated hypotheses and improper study designs, leading to unreliable results. Thus, the formulations of relevant research questions and verifiable hypotheses are crucial when beginning research. 12

CHARACTERISTICS OF GOOD RESEARCH QUESTIONS AND HYPOTHESES

Excellent research questions are specific and focused. These integrate collective data and observations to confirm or refute the subsequent hypotheses. Well-constructed hypotheses are based on previous reports and verify the research context. These are realistic, in-depth, sufficiently complex, and reproducible. More importantly, these hypotheses can be addressed and tested. 13

There are several characteristics of well-developed hypotheses. Good hypotheses are 1) empirically testable 7 , 10 , 11 , 13 ; 2) backed by preliminary evidence 9 ; 3) testable by ethical research 7 , 9 ; 4) based on original ideas 9 ; 5) have evidenced-based logical reasoning 10 ; and 6) can be predicted. 11 Good hypotheses can infer ethical and positive implications, indicating the presence of a relationship or effect relevant to the research theme. 7 , 11 These are initially developed from a general theory and branch into specific hypotheses by deductive reasoning. In the absence of a theory to base the hypotheses, inductive reasoning based on specific observations or findings form more general hypotheses. 10

TYPES OF RESEARCH QUESTIONS AND HYPOTHESES

Research questions and hypotheses are developed according to the type of research, which can be broadly classified into quantitative and qualitative research. We provide a summary of the types of research questions and hypotheses under quantitative and qualitative research categories in Table 1 .

Research questions in quantitative research

In quantitative research, research questions inquire about the relationships among variables being investigated and are usually framed at the start of the study. These are precise and typically linked to the subject population, dependent and independent variables, and research design. 1 Research questions may also attempt to describe the behavior of a population in relation to one or more variables, or describe the characteristics of variables to be measured ( descriptive research questions ). 1 , 5 , 14 These questions may also aim to discover differences between groups within the context of an outcome variable ( comparative research questions ), 1 , 5 , 14 or elucidate trends and interactions among variables ( relationship research questions ). 1 , 5 We provide examples of descriptive, comparative, and relationship research questions in quantitative research in Table 2 .

Hypotheses in quantitative research

In quantitative research, hypotheses predict the expected relationships among variables. 15 Relationships among variables that can be predicted include 1) between a single dependent variable and a single independent variable ( simple hypothesis ) or 2) between two or more independent and dependent variables ( complex hypothesis ). 4 , 11 Hypotheses may also specify the expected direction to be followed and imply an intellectual commitment to a particular outcome ( directional hypothesis ) 4 . On the other hand, hypotheses may not predict the exact direction and are used in the absence of a theory, or when findings contradict previous studies ( non-directional hypothesis ). 4 In addition, hypotheses can 1) define interdependency between variables ( associative hypothesis ), 4 2) propose an effect on the dependent variable from manipulation of the independent variable ( causal hypothesis ), 4 3) state a negative relationship between two variables ( null hypothesis ), 4 , 11 , 15 4) replace the working hypothesis if rejected ( alternative hypothesis ), 15 explain the relationship of phenomena to possibly generate a theory ( working hypothesis ), 11 5) involve quantifiable variables that can be tested statistically ( statistical hypothesis ), 11 6) or express a relationship whose interlinks can be verified logically ( logical hypothesis ). 11 We provide examples of simple, complex, directional, non-directional, associative, causal, null, alternative, working, statistical, and logical hypotheses in quantitative research, as well as the definition of quantitative hypothesis-testing research in Table 3 .

Research questions in qualitative research

Unlike research questions in quantitative research, research questions in qualitative research are usually continuously reviewed and reformulated. The central question and associated subquestions are stated more than the hypotheses. 15 The central question broadly explores a complex set of factors surrounding the central phenomenon, aiming to present the varied perspectives of participants. 15

There are varied goals for which qualitative research questions are developed. These questions can function in several ways, such as to 1) identify and describe existing conditions ( contextual research question s); 2) describe a phenomenon ( descriptive research questions ); 3) assess the effectiveness of existing methods, protocols, theories, or procedures ( evaluation research questions ); 4) examine a phenomenon or analyze the reasons or relationships between subjects or phenomena ( explanatory research questions ); or 5) focus on unknown aspects of a particular topic ( exploratory research questions ). 5 In addition, some qualitative research questions provide new ideas for the development of theories and actions ( generative research questions ) or advance specific ideologies of a position ( ideological research questions ). 1 Other qualitative research questions may build on a body of existing literature and become working guidelines ( ethnographic research questions ). Research questions may also be broadly stated without specific reference to the existing literature or a typology of questions ( phenomenological research questions ), may be directed towards generating a theory of some process ( grounded theory questions ), or may address a description of the case and the emerging themes ( qualitative case study questions ). 15 We provide examples of contextual, descriptive, evaluation, explanatory, exploratory, generative, ideological, ethnographic, phenomenological, grounded theory, and qualitative case study research questions in qualitative research in Table 4 , and the definition of qualitative hypothesis-generating research in Table 5 .

Qualitative studies usually pose at least one central research question and several subquestions starting with How or What . These research questions use exploratory verbs such as explore or describe . These also focus on one central phenomenon of interest, and may mention the participants and research site. 15

Hypotheses in qualitative research

Hypotheses in qualitative research are stated in the form of a clear statement concerning the problem to be investigated. Unlike in quantitative research where hypotheses are usually developed to be tested, qualitative research can lead to both hypothesis-testing and hypothesis-generating outcomes. 2 When studies require both quantitative and qualitative research questions, this suggests an integrative process between both research methods wherein a single mixed-methods research question can be developed. 1

FRAMEWORKS FOR DEVELOPING RESEARCH QUESTIONS AND HYPOTHESES

Research questions followed by hypotheses should be developed before the start of the study. 1 , 12 , 14 It is crucial to develop feasible research questions on a topic that is interesting to both the researcher and the scientific community. This can be achieved by a meticulous review of previous and current studies to establish a novel topic. Specific areas are subsequently focused on to generate ethical research questions. The relevance of the research questions is evaluated in terms of clarity of the resulting data, specificity of the methodology, objectivity of the outcome, depth of the research, and impact of the study. 1 , 5 These aspects constitute the FINER criteria (i.e., Feasible, Interesting, Novel, Ethical, and Relevant). 1 Clarity and effectiveness are achieved if research questions meet the FINER criteria. In addition to the FINER criteria, Ratan et al. described focus, complexity, novelty, feasibility, and measurability for evaluating the effectiveness of research questions. 14

The PICOT and PEO frameworks are also used when developing research questions. 1 The following elements are addressed in these frameworks, PICOT: P-population/patients/problem, I-intervention or indicator being studied, C-comparison group, O-outcome of interest, and T-timeframe of the study; PEO: P-population being studied, E-exposure to preexisting conditions, and O-outcome of interest. 1 Research questions are also considered good if these meet the “FINERMAPS” framework: Feasible, Interesting, Novel, Ethical, Relevant, Manageable, Appropriate, Potential value/publishable, and Systematic. 14

As we indicated earlier, research questions and hypotheses that are not carefully formulated result in unethical studies or poor outcomes. To illustrate this, we provide some examples of ambiguous research question and hypotheses that result in unclear and weak research objectives in quantitative research ( Table 6 ) 16 and qualitative research ( Table 7 ) 17 , and how to transform these ambiguous research question(s) and hypothesis(es) into clear and good statements.

a These statements were composed for comparison and illustrative purposes only.

b These statements are direct quotes from Higashihara and Horiuchi. 16

a This statement is a direct quote from Shimoda et al. 17

The other statements were composed for comparison and illustrative purposes only.

CONSTRUCTING RESEARCH QUESTIONS AND HYPOTHESES

To construct effective research questions and hypotheses, it is very important to 1) clarify the background and 2) identify the research problem at the outset of the research, within a specific timeframe. 9 Then, 3) review or conduct preliminary research to collect all available knowledge about the possible research questions by studying theories and previous studies. 18 Afterwards, 4) construct research questions to investigate the research problem. Identify variables to be accessed from the research questions 4 and make operational definitions of constructs from the research problem and questions. Thereafter, 5) construct specific deductive or inductive predictions in the form of hypotheses. 4 Finally, 6) state the study aims . This general flow for constructing effective research questions and hypotheses prior to conducting research is shown in Fig. 1 .

Research questions are used more frequently in qualitative research than objectives or hypotheses. 3 These questions seek to discover, understand, explore or describe experiences by asking “What” or “How.” The questions are open-ended to elicit a description rather than to relate variables or compare groups. The questions are continually reviewed, reformulated, and changed during the qualitative study. 3 Research questions are also used more frequently in survey projects than hypotheses in experiments in quantitative research to compare variables and their relationships.

Hypotheses are constructed based on the variables identified and as an if-then statement, following the template, ‘If a specific action is taken, then a certain outcome is expected.’ At this stage, some ideas regarding expectations from the research to be conducted must be drawn. 18 Then, the variables to be manipulated (independent) and influenced (dependent) are defined. 4 Thereafter, the hypothesis is stated and refined, and reproducible data tailored to the hypothesis are identified, collected, and analyzed. 4 The hypotheses must be testable and specific, 18 and should describe the variables and their relationships, the specific group being studied, and the predicted research outcome. 18 Hypotheses construction involves a testable proposition to be deduced from theory, and independent and dependent variables to be separated and measured separately. 3 Therefore, good hypotheses must be based on good research questions constructed at the start of a study or trial. 12

In summary, research questions are constructed after establishing the background of the study. Hypotheses are then developed based on the research questions. Thus, it is crucial to have excellent research questions to generate superior hypotheses. In turn, these would determine the research objectives and the design of the study, and ultimately, the outcome of the research. 12 Algorithms for building research questions and hypotheses are shown in Fig. 2 for quantitative research and in Fig. 3 for qualitative research.

EXAMPLES OF RESEARCH QUESTIONS FROM PUBLISHED ARTICLES

• EXAMPLE 1. Descriptive research question (quantitative research)
• - Presents research variables to be assessed (distinct phenotypes and subphenotypes)
• “BACKGROUND: Since COVID-19 was identified, its clinical and biological heterogeneity has been recognized. Identifying COVID-19 phenotypes might help guide basic, clinical, and translational research efforts.
• RESEARCH QUESTION: Does the clinical spectrum of patients with COVID-19 contain distinct phenotypes and subphenotypes? ” 19
• EXAMPLE 2. Relationship research question (quantitative research)
• - Shows interactions between dependent variable (static postural control) and independent variable (peripheral visual field loss)
• “Background: Integration of visual, vestibular, and proprioceptive sensations contributes to postural control. People with peripheral visual field loss have serious postural instability. However, the directional specificity of postural stability and sensory reweighting caused by gradual peripheral visual field loss remain unclear.
• Research question: What are the effects of peripheral visual field loss on static postural control ?” 20
• EXAMPLE 3. Comparative research question (quantitative research)
• - Clarifies the difference among groups with an outcome variable (patients enrolled in COMPERA with moderate PH or severe PH in COPD) and another group without the outcome variable (patients with idiopathic pulmonary arterial hypertension (IPAH))
• “BACKGROUND: Pulmonary hypertension (PH) in COPD is a poorly investigated clinical condition.
• RESEARCH QUESTION: Which factors determine the outcome of PH in COPD?
• STUDY DESIGN AND METHODS: We analyzed the characteristics and outcome of patients enrolled in the Comparative, Prospective Registry of Newly Initiated Therapies for Pulmonary Hypertension (COMPERA) with moderate or severe PH in COPD as defined during the 6th PH World Symposium who received medical therapy for PH and compared them with patients with idiopathic pulmonary arterial hypertension (IPAH) .” 21
• EXAMPLE 4. Exploratory research question (qualitative research)
• - Explores areas that have not been fully investigated (perspectives of families and children who receive care in clinic-based child obesity treatment) to have a deeper understanding of the research problem
• “Problem: Interventions for children with obesity lead to only modest improvements in BMI and long-term outcomes, and data are limited on the perspectives of families of children with obesity in clinic-based treatment. This scoping review seeks to answer the question: What is known about the perspectives of families and children who receive care in clinic-based child obesity treatment? This review aims to explore the scope of perspectives reported by families of children with obesity who have received individualized outpatient clinic-based obesity treatment.” 22
• EXAMPLE 5. Relationship research question (quantitative research)
• - Defines interactions between dependent variable (use of ankle strategies) and independent variable (changes in muscle tone)
• “Background: To maintain an upright standing posture against external disturbances, the human body mainly employs two types of postural control strategies: “ankle strategy” and “hip strategy.” While it has been reported that the magnitude of the disturbance alters the use of postural control strategies, it has not been elucidated how the level of muscle tone, one of the crucial parameters of bodily function, determines the use of each strategy. We have previously confirmed using forward dynamics simulations of human musculoskeletal models that an increased muscle tone promotes the use of ankle strategies. The objective of the present study was to experimentally evaluate a hypothesis: an increased muscle tone promotes the use of ankle strategies. Research question: Do changes in the muscle tone affect the use of ankle strategies ?” 23

EXAMPLES OF HYPOTHESES IN PUBLISHED ARTICLES

• EXAMPLE 1. Working hypothesis (quantitative research)
• - A hypothesis that is initially accepted for further research to produce a feasible theory
• “As fever may have benefit in shortening the duration of viral illness, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response when taken during the early stages of COVID-19 illness .” 24
• “In conclusion, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response . The difference in perceived safety of these agents in COVID-19 illness could be related to the more potent efficacy to reduce fever with ibuprofen compared to acetaminophen. Compelling data on the benefit of fever warrant further research and review to determine when to treat or withhold ibuprofen for early stage fever for COVID-19 and other related viral illnesses .” 24
• EXAMPLE 2. Exploratory hypothesis (qualitative research)
• - Explores particular areas deeper to clarify subjective experience and develop a formal hypothesis potentially testable in a future quantitative approach
• “We hypothesized that when thinking about a past experience of help-seeking, a self distancing prompt would cause increased help-seeking intentions and more favorable help-seeking outcome expectations .” 25
• “Conclusion
• Although a priori hypotheses were not supported, further research is warranted as results indicate the potential for using self-distancing approaches to increasing help-seeking among some people with depressive symptomatology.” 25
• EXAMPLE 3. Hypothesis-generating research to establish a framework for hypothesis testing (qualitative research)
• “We hypothesize that compassionate care is beneficial for patients (better outcomes), healthcare systems and payers (lower costs), and healthcare providers (lower burnout). ” 26
• Compassionomics is the branch of knowledge and scientific study of the effects of compassionate healthcare. Our main hypotheses are that compassionate healthcare is beneficial for (1) patients, by improving clinical outcomes, (2) healthcare systems and payers, by supporting financial sustainability, and (3) HCPs, by lowering burnout and promoting resilience and well-being. The purpose of this paper is to establish a scientific framework for testing the hypotheses above . If these hypotheses are confirmed through rigorous research, compassionomics will belong in the science of evidence-based medicine, with major implications for all healthcare domains.” 26
• EXAMPLE 4. Statistical hypothesis (quantitative research)
• - An assumption is made about the relationship among several population characteristics ( gender differences in sociodemographic and clinical characteristics of adults with ADHD ). Validity is tested by statistical experiment or analysis ( chi-square test, Students t-test, and logistic regression analysis)
• “Our research investigated gender differences in sociodemographic and clinical characteristics of adults with ADHD in a Japanese clinical sample. Due to unique Japanese cultural ideals and expectations of women's behavior that are in opposition to ADHD symptoms, we hypothesized that women with ADHD experience more difficulties and present more dysfunctions than men . We tested the following hypotheses: first, women with ADHD have more comorbidities than men with ADHD; second, women with ADHD experience more social hardships than men, such as having less full-time employment and being more likely to be divorced.” 27
• “Statistical Analysis
• ( text omitted ) Between-gender comparisons were made using the chi-squared test for categorical variables and Students t-test for continuous variables…( text omitted ). A logistic regression analysis was performed for employment status, marital status, and comorbidity to evaluate the independent effects of gender on these dependent variables.” 27

EXAMPLES OF HYPOTHESIS AS WRITTEN IN PUBLISHED ARTICLES IN RELATION TO OTHER PARTS

• EXAMPLE 1. Background, hypotheses, and aims are provided
• “Pregnant women need skilled care during pregnancy and childbirth, but that skilled care is often delayed in some countries …( text omitted ). The focused antenatal care (FANC) model of WHO recommends that nurses provide information or counseling to all pregnant women …( text omitted ). Job aids are visual support materials that provide the right kind of information using graphics and words in a simple and yet effective manner. When nurses are not highly trained or have many work details to attend to, these job aids can serve as a content reminder for the nurses and can be used for educating their patients (Jennings, Yebadokpo, Affo, & Agbogbe, 2010) ( text omitted ). Importantly, additional evidence is needed to confirm how job aids can further improve the quality of ANC counseling by health workers in maternal care …( text omitted )” 28
• “ This has led us to hypothesize that the quality of ANC counseling would be better if supported by job aids. Consequently, a better quality of ANC counseling is expected to produce higher levels of awareness concerning the danger signs of pregnancy and a more favorable impression of the caring behavior of nurses .” 28
• “This study aimed to examine the differences in the responses of pregnant women to a job aid-supported intervention during ANC visit in terms of 1) their understanding of the danger signs of pregnancy and 2) their impression of the caring behaviors of nurses to pregnant women in rural Tanzania.” 28
• EXAMPLE 2. Background, hypotheses, and aims are provided
• “We conducted a two-arm randomized controlled trial (RCT) to evaluate and compare changes in salivary cortisol and oxytocin levels of first-time pregnant women between experimental and control groups. The women in the experimental group touched and held an infant for 30 min (experimental intervention protocol), whereas those in the control group watched a DVD movie of an infant (control intervention protocol). The primary outcome was salivary cortisol level and the secondary outcome was salivary oxytocin level.” 29
• “ We hypothesize that at 30 min after touching and holding an infant, the salivary cortisol level will significantly decrease and the salivary oxytocin level will increase in the experimental group compared with the control group .” 29
• EXAMPLE 3. Background, aim, and hypothesis are provided
• “In countries where the maternal mortality ratio remains high, antenatal education to increase Birth Preparedness and Complication Readiness (BPCR) is considered one of the top priorities [1]. BPCR includes birth plans during the antenatal period, such as the birthplace, birth attendant, transportation, health facility for complications, expenses, and birth materials, as well as family coordination to achieve such birth plans. In Tanzania, although increasing, only about half of all pregnant women attend an antenatal clinic more than four times [4]. Moreover, the information provided during antenatal care (ANC) is insufficient. In the resource-poor settings, antenatal group education is a potential approach because of the limited time for individual counseling at antenatal clinics.” 30
• “This study aimed to evaluate an antenatal group education program among pregnant women and their families with respect to birth-preparedness and maternal and infant outcomes in rural villages of Tanzania.” 30
• “ The study hypothesis was if Tanzanian pregnant women and their families received a family-oriented antenatal group education, they would (1) have a higher level of BPCR, (2) attend antenatal clinic four or more times, (3) give birth in a health facility, (4) have less complications of women at birth, and (5) have less complications and deaths of infants than those who did not receive the education .” 30

Research questions and hypotheses are crucial components to any type of research, whether quantitative or qualitative. These questions should be developed at the very beginning of the study. Excellent research questions lead to superior hypotheses, which, like a compass, set the direction of research, and can often determine the successful conduct of the study. Many research studies have floundered because the development of research questions and subsequent hypotheses was not given the thought and meticulous attention needed. The development of research questions and hypotheses is an iterative process based on extensive knowledge of the literature and insightful grasp of the knowledge gap. Focused, concise, and specific research questions provide a strong foundation for constructing hypotheses which serve as formal predictions about the research outcomes. Research questions and hypotheses are crucial elements of research that should not be overlooked. They should be carefully thought of and constructed when planning research. This avoids unethical studies and poor outcomes by defining well-founded objectives that determine the design, course, and outcome of the study.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Barroga E, Matanguihan GJ.
• Methodology: Barroga E, Matanguihan GJ.
• Writing - original draft: Barroga E, Matanguihan GJ.
• Writing - review & editing: Barroga E, Matanguihan GJ.

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Home Market Research

Quantitative Research: What It Is, Practices & Methods

Quantitative research involves analyzing and gathering numerical data to uncover trends, calculate averages, evaluate relationships, and derive overarching insights. It’s used in various fields, including the natural and social sciences. Quantitative data analysis employs statistical techniques for processing and interpreting numeric data.

Research designs in the quantitative realm outline how data will be collected and analyzed with methods like experiments and surveys. Qualitative methods complement quantitative research by focusing on non-numerical data, adding depth to understanding. Data collection methods can be qualitative or quantitative, depending on research goals. Researchers often use a combination of both approaches to gain a comprehensive understanding of phenomena.

What is Quantitative Research?

Quantitative research is a systematic investigation of phenomena by gathering quantifiable data and performing statistical, mathematical, or computational techniques. Quantitative research collects statistically significant information from existing and potential customers using sampling methods and sending out online surveys , online polls , and questionnaires , for example.

One of the main characteristics of this type of research is that the results can be depicted in numerical form. After carefully collecting structured observations and understanding these numbers, it’s possible to predict the future of a product or service, establish causal relationships or Causal Research , and make changes accordingly. Quantitative research primarily centers on the analysis of numerical data and utilizes inferential statistics to derive conclusions that can be extrapolated to the broader population.

An example of a quantitative research study is the survey conducted to understand how long a doctor takes to tend to a patient when the patient walks into the hospital. A patient satisfaction survey can be administered to ask questions like how long a doctor takes to see a patient, how often a patient walks into a hospital, and other such questions, which are dependent variables in the research. This kind of research method is often employed in the social sciences, and it involves using mathematical frameworks and theories to effectively present data, ensuring that the results are logical, statistically sound, and unbiased.

Data collection in quantitative research uses a structured method and is typically conducted on larger samples representing the entire population. Researchers use quantitative methods to collect numerical data, which is then subjected to statistical analysis to determine statistically significant findings. This approach is valuable in both experimental research and social research, as it helps in making informed decisions and drawing reliable conclusions based on quantitative data.

Quantitative Research Characteristics

Quantitative research has several unique characteristics that make it well-suited for specific projects. Let’s explore the most crucial of these characteristics so that you can consider them when planning your next research project:

• Structured tools: Quantitative research relies on structured tools such as surveys, polls, or questionnaires to gather quantitative data . Using such structured methods helps collect in-depth and actionable numerical data from the survey respondents, making it easier to perform data analysis.
• Sample size: Quantitative research is conducted on a significant sample size  representing the target market . Appropriate Survey Sampling methods, a fundamental aspect of quantitative research methods, must be employed when deriving the sample to fortify the research objective and ensure the reliability of the results.
• Close-ended questions: Closed-ended questions , specifically designed to align with the research objectives, are a cornerstone of quantitative research. These questions facilitate the collection of quantitative data and are extensively used in data collection processes.
• Prior studies: Before collecting feedback from respondents, researchers often delve into previous studies related to the research topic. This preliminary research helps frame the study effectively and ensures the data collection process is well-informed.
• Quantitative data: Typically, quantitative data is represented using tables, charts, graphs, or other numerical forms. This visual representation aids in understanding the collected data and is essential for rigorous data analysis, a key component of quantitative research methods.
• Generalization of results: One of the strengths of quantitative research is its ability to generalize results to the entire population. It means that the findings derived from a sample can be extrapolated to make informed decisions and take appropriate actions for improvement based on numerical data analysis.

Quantitative Research Methods

Quantitative research methods are systematic approaches used to gather and analyze numerical data to understand and draw conclusions about a phenomenon or population. Here are the quantitative research methods:

• Primary quantitative research methods
• Secondary quantitative research methods

Primary Quantitative Research Methods

Primary quantitative research is the most widely used method of conducting market research. The distinct feature of primary research is that the researcher focuses on collecting data directly rather than depending on data collected from previously done research. Primary quantitative research design can be broken down into three further distinctive tracks and the process flow. They are:

A. Techniques and Types of Studies

There are multiple types of primary quantitative research. They can be distinguished into the four following distinctive methods, which are:

01. Survey Research

Survey Research is fundamental for all quantitative outcome research methodologies and studies. Surveys are used to ask questions to a sample of respondents, using various types such as online polls, online surveys, paper questionnaires, web-intercept surveys , etc. Every small and big organization intends to understand what their customers think about their products and services, how well new features are faring in the market, and other such details.

By conducting survey research, an organization can ask multiple survey questions , collect data from a pool of customers, and analyze this collected data to produce numerical results. It is the first step towards collecting data for any research. You can use single ease questions . A single-ease question is a straightforward query that elicits a concise and uncomplicated response.

This type of research can be conducted with a specific target audience group and also can be conducted across multiple groups along with comparative analysis . A prerequisite for this type of research is that the sample of respondents must have randomly selected members. This way, a researcher can easily maintain the accuracy of the obtained results as a huge variety of respondents will be addressed using random selection. 

Traditionally, survey research was conducted face-to-face or via phone calls. Still, with the progress made by online mediums such as email or social media, survey research has also spread to online mediums.There are two types of surveys , either of which can be chosen based on the time in hand and the kind of data required:

Cross-sectional surveys: Cross-sectional surveys are observational surveys conducted in situations where the researcher intends to collect data from a sample of the target population at a given point in time. Researchers can evaluate various variables at a particular time. Data gathered using this type of survey is from people who depict similarity in all variables except the variables which are considered for research . Throughout the survey, this one variable will stay constant.

• Cross-sectional surveys are popular with retail, SMEs, and healthcare industries. Information is garnered without modifying any parameters in the variable ecosystem.
• Multiple samples can be analyzed and compared using a cross-sectional survey research method.
• Multiple variables can be evaluated using this type of survey research.
• The only disadvantage of cross-sectional surveys is that the cause-effect relationship of variables cannot be established as it usually evaluates variables at a particular time and not across a continuous time frame.

Longitudinal surveys: Longitudinal surveys are also observational surveys , but unlike cross-sectional surveys, longitudinal surveys are conducted across various time durations to observe a change in respondent behavior and thought processes. This time can be days, months, years, or even decades. For instance, a researcher planning to analyze the change in buying habits of teenagers over 5 years will conduct longitudinal surveys.

• In cross-sectional surveys, the same variables were evaluated at a given time, and in longitudinal surveys, different variables can be analyzed at different intervals.
• Longitudinal surveys are extensively used in the field of medicine and applied sciences. Apart from these two fields, they are also used to observe a change in the market trend analysis , analyze customer satisfaction, or gain feedback on products/services.
• In situations where the sequence of events is highly essential, longitudinal surveys are used.
• Researchers say that when research subjects need to be thoroughly inspected before concluding, they rely on longitudinal surveys.

02. Correlational Research

A comparison between two entities is invariable. Correlation research is conducted to establish a relationship between two closely-knit entities and how one impacts the other, and what changes are eventually observed. This research method is carried out to give value to naturally occurring relationships, and a minimum of two different groups are required to conduct this quantitative research method successfully. Without assuming various aspects, a relationship between two groups or entities must be established.

Researchers use this quantitative research design to correlate two or more variables using mathematical analysis methods. Patterns, relationships, and trends between variables are concluded as they exist in their original setup. The impact of one of these variables on the other is observed, along with how it changes the relationship between the two variables. Researchers tend to manipulate one of the variables to attain the desired results.

Ideally, it is advised not to make conclusions merely based on correlational research. This is because it is not mandatory that if two variables are in sync that they are interrelated.

Example of Correlational Research Questions :

• The relationship between stress and depression.
• The equation between fame and money.
• The relation between activities in a third-grade class and its students.

03. Causal-comparative Research

This research method mainly depends on the factor of comparison. Also called quasi-experimental research , this quantitative research method is used by researchers to conclude the cause-effect equation between two or more variables, where one variable is dependent on the other independent variable. The independent variable is established but not manipulated, and its impact on the dependent variable is observed. These variables or groups must be formed as they exist in the natural setup. As the dependent and independent variables will always exist in a group, it is advised that the conclusions are carefully established by keeping all the factors in mind.

Causal-comparative research is not restricted to the statistical analysis of two variables but extends to analyzing how various variables or groups change under the influence of the same changes. This research is conducted irrespective of the type of relationship that exists between two or more variables. Statistical analysis plan is used to present the outcome using this quantitative research method.

Example of Causal-Comparative Research Questions:

• The impact of drugs on a teenager. The effect of good education on a freshman. The effect of substantial food provision in the villages of Africa.

04. Experimental Research

Also known as true experimentation, this research method relies on a theory. As the name suggests, experimental research is usually based on one or more theories. This theory has yet to be proven before and is merely a supposition. In experimental research, an analysis is done around proving or disproving the statement. This research method is used in natural sciences. Traditional research methods are more effective than modern techniques.

There can be multiple theories in experimental research. A theory is a statement that can be verified or refuted.

After establishing the statement, efforts are made to understand whether it is valid or invalid. This quantitative research method is mainly used in natural or social sciences as various statements must be proved right or wrong.

• Traditional research methods are more effective than modern techniques.
• Systematic teaching schedules help children who struggle to cope with the course.
• It is a boon to have responsible nursing staff for ailing parents.

B. Data Collection Methodologies

The second major step in primary quantitative research is data collection. Data collection can be divided into sampling methods and data collection using surveys and polls.

01. Data Collection Methodologies: Sampling Methods

There are two main sampling methods for quantitative research: Probability and Non-probability sampling .

Probability sampling: A theory of probability is used to filter individuals from a population and create samples in probability sampling . Participants of a sample are chosen by random selection processes. Each target audience member has an equal opportunity to be selected in the sample.

There are four main types of probability sampling:

• Simple random sampling: As the name indicates, simple random sampling is nothing but a random selection of elements for a sample. This sampling technique is implemented where the target population is considerably large.
• Stratified random sampling: In the stratified random sampling method , a large population is divided into groups (strata), and members of a sample are chosen randomly from these strata. The various segregated strata should ideally not overlap one another.
• Cluster sampling: Cluster sampling is a probability sampling method using which the main segment is divided into clusters, usually using geographic segmentation and demographic segmentation parameters.
• Systematic sampling: Systematic sampling is a technique where the starting point of the sample is chosen randomly, and all the other elements are chosen using a fixed interval. This interval is calculated by dividing the population size by the target sample size.

Non-probability sampling: Non-probability sampling is where the researcher’s knowledge and experience are used to create samples. Because of the researcher’s involvement, not all the target population members have an equal probability of being selected to be a part of a sample.

There are five non-probability sampling models:

• Convenience sampling: In convenience sampling , elements of a sample are chosen only due to one prime reason: their proximity to the researcher. These samples are quick and easy to implement as there is no other parameter of selection involved.
• Consecutive sampling: Consecutive sampling is quite similar to convenience sampling, except for the fact that researchers can choose a single element or a group of samples and conduct research consecutively over a significant period and then perform the same process with other samples.
• Quota sampling: Using quota sampling , researchers can select elements using their knowledge of target traits and personalities to form strata. Members of various strata can then be chosen to be a part of the sample as per the researcher’s understanding.
• Snowball sampling: Snowball sampling is conducted with target audiences who are difficult to contact and get information. It is popular in cases where the target audience for analysis research is rare to put together.
• Judgmental sampling: Judgmental sampling is a non-probability sampling method where samples are created only based on the researcher’s experience and research skill .

02. Data collection methodologies: Using surveys & polls

Once the sample is determined, then either surveys or polls can be distributed to collect the data for quantitative research.

Using surveys for primary quantitative research

A survey is defined as a research method used for collecting data from a pre-defined group of respondents to gain information and insights on various topics of interest. The ease of survey distribution and the wide number of people it can reach depending on the research time and objective makes it one of the most important aspects of conducting quantitative research.

Fundamental levels of measurement – nominal, ordinal, interval, and ratio scales

Four measurement scales are fundamental to creating a multiple-choice question in a survey. They are nominal, ordinal, interval, and ratio measurement scales without the fundamentals of which no multiple-choice questions can be created. Hence, it is crucial to understand these measurement levels to develop a robust survey.

Use of different question types

To conduct quantitative research, close-ended questions must be used in a survey. They can be a mix of multiple question types, including multiple-choice questions like semantic differential scale questions , rating scale questions , etc.

Survey Distribution and Survey Data Collection

In the above, we have seen the process of building a survey along with the research design to conduct primary quantitative research. Survey distribution to collect data is the other important aspect of the survey process. There are different ways of survey distribution. Some of the most commonly used methods are:

• Email: Sending a survey via email is the most widely used and effective survey distribution method. This method’s response rate is high because the respondents know your brand. You can use the QuestionPro email management feature to send out and collect survey responses.
• Buy respondents: Another effective way to distribute a survey and conduct primary quantitative research is to use a sample. Since the respondents are knowledgeable and are on the panel by their own will, responses are much higher.
• Embed survey on a website: Embedding a survey on a website increases a high number of responses as the respondent is already in close proximity to the brand when the survey pops up.
• Social distribution: Using social media to distribute the survey aids in collecting a higher number of responses from the people that are aware of the brand.
• QR code: QuestionPro QR codes store the URL for the survey. You can print/publish this code in magazines, signs, business cards, or on just about any object/medium.
• SMS survey: The SMS survey is a quick and time-effective way to collect a high number of responses.
• Offline Survey App: The QuestionPro App allows users to circulate surveys quickly, and the responses can be collected both online and offline.

Survey example

An example of a survey is a short customer satisfaction (CSAT) survey that can quickly be built and deployed to collect feedback about what the customer thinks about a brand and how satisfied and referenceable the brand is.

Using polls for primary quantitative research

Polls are a method to collect feedback using close-ended questions from a sample. The most commonly used types of polls are election polls and exit polls . Both of these are used to collect data from a large sample size but using basic question types like multiple-choice questions.

C. Data Analysis Techniques

The third aspect of primary quantitative research design is data analysis . After collecting raw data, there must be an analysis of this data to derive statistical inferences from this research. It is important to relate the results to the research objective and establish the statistical relevance of the results.

Remember to consider aspects of research that were not considered for the data collection process and report the difference between what was planned vs. what was actually executed.

It is then required to select precise Statistical Analysis Methods , such as SWOT, Conjoint, Cross-tabulation, etc., to analyze the quantitative data.

• SWOT analysis: SWOT Analysis stands for the acronym of Strengths, Weaknesses, Opportunities, and Threat analysis. Organizations use this statistical analysis technique to evaluate their performance internally and externally to develop effective strategies for improvement.
• Conjoint Analysis: Conjoint Analysis is a market analysis method to learn how individuals make complicated purchasing decisions. Trade-offs are involved in an individual’s daily activities, and these reflect their ability to decide from a complex list of product/service options.
• Cross-tabulation: Cross-tabulation is one of the preliminary statistical market analysis methods which establishes relationships, patterns, and trends within the various parameters of the research study.
• TURF Analysis: TURF Analysis , an acronym for Totally Unduplicated Reach and Frequency Analysis, is executed in situations where the reach of a favorable communication source is to be analyzed along with the frequency of this communication. It is used for understanding the potential of a target market.

Inferential statistics methods such as confidence interval, the margin of error, etc., can then be used to provide results.

Secondary Quantitative Research Methods

Secondary quantitative research or desk research is a research method that involves using already existing data or secondary data. Existing data is summarized and collated to increase the overall effectiveness of the research.

This research method involves collecting quantitative data from existing data sources like the internet, government resources, libraries, research reports, etc. Secondary quantitative research helps to validate the data collected from primary quantitative research and aid in strengthening or proving, or disproving previously collected data.

The following are five popularly used secondary quantitative research methods:

• Data available on the internet: With the high penetration of the internet and mobile devices, it has become increasingly easy to conduct quantitative research using the internet. Information about most research topics is available online, and this aids in boosting the validity of primary quantitative data.
• Government and non-government sources: Secondary quantitative research can also be conducted with the help of government and non-government sources that deal with market research reports. This data is highly reliable and in-depth and hence, can be used to increase the validity of quantitative research design.
• Public libraries: Now a sparingly used method of conducting quantitative research, it is still a reliable source of information, though. Public libraries have copies of important research that was conducted earlier. They are a storehouse of valuable information and documents from which information can be extracted.
• Educational institutions: Educational institutions conduct in-depth research on multiple topics, and hence, the reports that they publish are an important source of validation in quantitative research.
• Commercial information sources: Local newspapers, journals, magazines, radio, and TV stations are great sources to obtain data for secondary quantitative research. These commercial information sources have in-depth, first-hand information on market research, demographic segmentation, and similar subjects.

Quantitative Research Examples

Some examples of quantitative research are:

• A customer satisfaction template can be used if any organization would like to conduct a customer satisfaction (CSAT) survey . Through this kind of survey, an organization can collect quantitative data and metrics on the goodwill of the brand or organization in the customer’s mind based on multiple parameters such as product quality, pricing, customer experience, etc. This data can be collected by asking a net promoter score (NPS) question , matrix table questions, etc. that provide data in the form of numbers that can be analyzed and worked upon.
• Another example of quantitative research is an organization that conducts an event, collecting feedback from attendees about the value they see from the event. By using an event survey , the organization can collect actionable feedback about the satisfaction levels of customers during various phases of the event such as the sales, pre and post-event, the likelihood of recommending the organization to their friends and colleagues, hotel preferences for the future events and other such questions.

What are the Advantages of Quantitative Research?

There are many advantages to quantitative research. Some of the major advantages of why researchers use this method in market research are:

Collect Reliable and Accurate Data:

Quantitative research is a powerful method for collecting reliable and accurate quantitative data. Since data is collected, analyzed, and presented in numbers, the results obtained are incredibly reliable and objective. Numbers do not lie and offer an honest and precise picture of the conducted research without discrepancies. In situations where a researcher aims to eliminate bias and predict potential conflicts, quantitative research is the method of choice.

Quick Data Collection:

Quantitative research involves studying a group of people representing a larger population. Researchers use a survey or another quantitative research method to efficiently gather information from these participants, making the process of analyzing the data and identifying patterns faster and more manageable through the use of statistical analysis. This advantage makes quantitative research an attractive option for projects with time constraints.

Wider Scope of Data Analysis:

Quantitative research, thanks to its utilization of statistical methods, offers an extensive range of data collection and analysis. Researchers can delve into a broader spectrum of variables and relationships within the data, enabling a more thorough comprehension of the subject under investigation. This expanded scope is precious when dealing with complex research questions that require in-depth numerical analysis.

Eliminate Bias:

One of the significant advantages of quantitative research is its ability to eliminate bias. This research method leaves no room for personal comments or the biasing of results, as the findings are presented in numerical form. This objectivity makes the results fair and reliable in most cases, reducing the potential for researcher bias or subjectivity.

In summary, quantitative research involves collecting, analyzing, and presenting quantitative data using statistical analysis. It offers numerous advantages, including the collection of reliable and accurate data, quick data collection, a broader scope of data analysis, and the elimination of bias, making it a valuable approach in the field of research. When considering the benefits of quantitative research, it’s essential to recognize its strengths in contrast to qualitative methods and its role in collecting and analyzing numerical data for a more comprehensive understanding of research topics.

Best Practices to Conduct Quantitative Research

Here are some best practices for conducting quantitative research:

• Differentiate between quantitative and qualitative: Understand the difference between the two methodologies and apply the one that suits your needs best.
• Choose a suitable sample size: Ensure that you have a sample representative of your population and large enough to be statistically weighty.
• Keep your research goals clear and concise: Know your research goals before you begin data collection to ensure you collect the right amount and the right quantity of data.
• Keep the questions simple: Remember that you will be reaching out to a demographically wide audience. Pose simple questions for your respondents to understand easily.

Quantitative Research vs Qualitative Research

Quantitative research and qualitative research are two distinct approaches to conducting research, each with its own set of methods and objectives. Here’s a comparison of the two:

Quantitative Research

• Objective: The primary goal of quantitative research is to quantify and measure phenomena by collecting numerical data. It aims to test hypotheses, establish patterns, and generalize findings to a larger population.
• Data Collection: Quantitative research employs systematic and standardized approaches for data collection, including techniques like surveys, experiments, and observations that involve predefined variables. It is often collected from a large and representative sample.
• Data Analysis: Data is analyzed using statistical techniques, such as descriptive statistics, inferential statistics, and mathematical modeling. Researchers use statistical tests to draw conclusions and make generalizations based on numerical data.
• Sample Size: Quantitative research often involves larger sample sizes to ensure statistical significance and generalizability.
• Results: The results are typically presented in tables, charts, and statistical summaries, making them highly structured and objective.
• Generalizability: Researchers intentionally structure quantitative research to generate outcomes that can be helpful to a larger population, and they frequently seek to establish causative connections.
• Emphasis on Objectivity: Researchers aim to minimize bias and subjectivity, focusing on replicable and objective findings.

Qualitative Research

• Objective: Qualitative research seeks to gain a deeper understanding of the underlying motivations, behaviors, and experiences of individuals or groups. It explores the context and meaning of phenomena.
• Data Collection: Qualitative research employs adaptable and open-ended techniques for data collection, including methods like interviews, focus groups, observations, and content analysis. It allows participants to express their perspectives in their own words.
• Data Analysis: Data is analyzed through thematic analysis, content analysis, or grounded theory. Researchers focus on identifying patterns, themes, and insights in the data.
• Sample Size: Qualitative research typically involves smaller sample sizes due to the in-depth nature of data collection and analysis.
• Results: Findings are presented in narrative form, often in the participants’ own words. Results are subjective, context-dependent, and provide rich, detailed descriptions.
• Generalizability: Qualitative research does not aim for broad generalizability but focuses on in-depth exploration within a specific context. It provides a detailed understanding of a particular group or situation.
• Emphasis on Subjectivity: Researchers acknowledge the role of subjectivity and the researcher’s influence on the Research Process . Participant perspectives and experiences are central to the findings.

Researchers choose between quantitative and qualitative research methods based on their research objectives and the nature of the research question. Each approach has its advantages and drawbacks, and the decision between them hinges on the particular research objectives and the data needed to address research inquiries effectively.

Quantitative research is a structured way of collecting and analyzing data from various sources. Its purpose is to quantify the problem and understand its extent, seeking results that someone can project to a larger population.

Companies that use quantitative rather than qualitative research typically aim to measure magnitudes and seek objectively interpreted statistical results. So if you want to obtain quantitative data that helps you define the structured cause-and-effect relationship between the research problem and the factors, you should opt for this type of research.

At QuestionPro , we have various Best Data Collection Tools and features to conduct investigations of this type. You can create questionnaires and distribute them through our various methods. We also have sample services or various questions to guarantee the success of your study and the quality of the collected data.

Quantitative research is a systematic and structured approach to studying phenomena that involves the collection of measurable data and the application of statistical, mathematical, or computational techniques for analysis.

Quantitative research is characterized by structured tools like surveys, substantial sample sizes, closed-ended questions, reliance on prior studies, data presented numerically, and the ability to generalize findings to the broader population.

The two main methods of quantitative research are Primary quantitative research methods, involving data collection directly from sources, and Secondary quantitative research methods, which utilize existing data for analysis.

1.Surveying to measure employee engagement with numerical rating scales. 2.Analyzing sales data to identify trends in product demand and market share. 4.Examining test scores to assess the impact of a new teaching method on student performance. 4.Using website analytics to track user behavior and conversion rates for an online store.

1.Differentiate between quantitative and qualitative approaches. 2.Choose a representative sample size. 3.Define clear research goals before data collection. 4.Use simple and easily understandable survey questions.

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Quantitative and Qualitative Research

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Quantitative methodology is the dominant research framework in the social sciences. It refers to a set of strategies, techniques and assumptions used to study psychological, social and economic processes through the exploration of numeric patterns . Quantitative research gathers a range of numeric data. Some of the numeric data is intrinsically quantitative (e.g. personal income), while in other cases the numeric structure is  imposed (e.g. ‘On a scale from 1 to 10, how depressed did you feel last week?’). The collection of quantitative information allows researchers to conduct simple to extremely sophisticated statistical analyses that aggregate the data (e.g. averages, percentages), show relationships among the data (e.g. ‘Students with lower grade point averages tend to score lower on a depression scale’) or compare across aggregated data (e.g. the USA has a higher gross domestic product than Spain). Quantitative research includes methodologies such as questionnaires, structured observations or experiments and stands in contrast to qualitative research. Qualitative research involves the collection and analysis of narratives and/or open-ended observations through methodologies such as interviews, focus groups or ethnographies.

Coghlan, D., Brydon-Miller, M. (2014).  The SAGE encyclopedia of action research  (Vols. 1-2). London, : SAGE Publications Ltd doi: 10.4135/9781446294406

What is the purpose of quantitative research?

The purpose of quantitative research is to generate knowledge and create understanding about the social world. Quantitative research is used by social scientists, including communication researchers, to observe phenomena or occurrences affecting individuals. Social scientists are concerned with the study of people. Quantitative research is a way to learn about a particular group of people, known as a sample population. Using scientific inquiry, quantitative research relies on data that are observed or measured to examine questions about the sample population.

Allen, M. (2017).  The SAGE encyclopedia of communication research methods  (Vols. 1-4). Thousand Oaks, CA: SAGE Publications, Inc doi: 10.4135/9781483381411

How do I know if the study is a quantitative design?  What type of quantitative study is it?

Quantitative Research Designs: Descriptive non-experimental, Quasi-experimental or Experimental?

Studies do not always explicitly state what kind of research design is being used.  You will need to know how to decipher which design type is used.  The following video will help you determine the quantitative design type.

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Your ultimate guide to quantitative research.

12 min read You may be already using quantitative research and want to check your understanding, or you may be starting from the beginning. Here’s an exploration of this research method and how you can best use it for maximum effect for your business.

You may be already using quantitative research and want to check your understanding, or you may be starting from the beginning. Here’s an exploration of this research method and how you can best use it for maximum effect for your business.

What is quantitative research?

Quantitative is the research method of collecting quantitative data – this is data that can be converted into numbers or numerical data, which can be easily quantified, compared, and analyzed.

Quantitative research deals with primary and secondary sources where data is represented in numerical form. This can include closed-question poll results, statistics, and census information or demographic data .

Quantitative data tends to be used when researchers are interested in understanding a particular moment in time and examining data sets over time to find trends and patterns.

To collect numerical data, surveys are often employed as one of the main research methods to source first-hand information in primary research . Quantitative research can also come from third-party research studies .

Quantitative research is widely used in the realms of social sciences, such as biology, chemistry, psychology, economics, sociology, and marketing .

Research teams collect data that is significant to proving or disproving a hypothesis research question – known as the research objective. When they collect quantitative data, researchers will aim to use a sample size that is representative of the total population of the target market they’re interested in.

Then the data collected will be manually or automatically stored and compared for insights.

Free eBook: The ultimate guide to conducting market research

Quantitative vs qualitative research

While the quantitative research definition focuses on numerical data, qualitative research is defined as data that supplies non-numerical information.

Quantitative research focuses on the thoughts, feelings, and values of a participant , to understand why people act in the way they do . They result in data types like quotes, symbols, images, and written testimonials.

These data types tell researchers subjective information, which can help us assign people into categories, such as a participant’s religion, gender , social class, political alignment, likely favored products to buy, or their preferred training learning style.

For this reason, qualitative research is often used in social research, as this gives a window into the behavior and actions of people.

In general, if you’re interested in measuring something or testing a hypothesis, use quantitative methods. If you want to explore ideas, thoughts, and meanings, use qualitative methods.

However, quantitative and qualitative research methods are both recommended when you’re looking to understand a point in time, while also finding out the reason behind the facts.

Quantitative research data collection methods

Quantitative research methods can use structured research instruments like:

• Surveys : A survey is a simple-to-create and easy-to-distribute research method , which helps gather information from large groups of participants quickly. Traditionally, paper-based surveys can now be made online, so costs can stay quite low.

Quantitative questions tend to be closed questions that ask for a numerical result, based on a range of options, or a yes/no answer that can be tallied quickly.

• Face-to-face or phone interviews: Interviews are a great way to connect with participants , though they require time from the research team to set up and conduct.

Researchers may also have issues connecting with participants in different geographical regions . The researcher uses a set of predefined close-ended questions, which ask for yes/no or numerical values.

• Polls: Polls can be a shorter version of surveys , used to get a ‘flavor’ of what the current situation is with participants. Online polls can be shared easily, though polls are best used with simple questions that request a range or a yes/no answer.

Quantitative data is the opposite of qualitative research, another dominant framework for research in the social sciences, explored further below.

Quantitative data types

Quantitative research methods often deliver the following data types:

• Test Scores
• Percent of training course completed
• Performance score out of 100
• Number of support calls active
• Customer Net Promoter Score (NPS)

When gathering numerical data, the emphasis is on how specific the data is, and whether they can provide an indication of what ‘is’ at the time of collection. Pre-existing statistical data can tell us what ‘was’ for the date and time range that it represented

Quantitative research design methods (with examples)

Quantitative research has a number of quantitative research designs you can choose from:

Descriptive

This design type describes the state of a data type is telling researchers, in its native environment. There won’t normally be a clearly defined research question to start with. Instead, data analysis will suggest a conclusion , which can become the hypothesis to investigate further.

Examples of descriptive quantitative design include:

• A description of child’s Christmas gifts they received that year
• A description of what businesses sell the most of during Black Friday
• A description of a product issue being experienced by a customer

Correlational

This design type looks at two or more data types, the relationship between them, and the extent that they differ or align. This does not look at the causal links deeper – instead statistical analysis looks at the variables in a natural environment.

Examples of correlational quantitative design include:

• The relationship between a child’s Christmas gifts and their perceived happiness level
• The relationship between a business’ sales during Black Friday and the total revenue generated over the year
• The relationship between a customer’s product issue and the reputation of the product

Causal-Comparative/Quasi-Experimental

This design type looks at two or more data types and tries to explain any relationship and differences between them, using a cause-effect analysis. The research is carried out in a near-natural environment, where information is gathered from two groups – a naturally occurring group that matches the original natural environment, and one that is not naturally present.

This allows for causal links to be made, though they might not be correct, as other variables may have an impact on results.

Examples of causal-comparative/quasi-experimental quantitative design include:

• The effect of children’s Christmas gifts on happiness
• The effect of Black Friday sales figures on the productivity of company yearly sales
• The effect of product issues on the public perception of a product

Experimental Research

This design type looks to make a controlled environment in which two or more variables are observed to understand the exact cause and effect they have. This becomes a quantitative research study, where data types are manipulated to assess the effect they have. The participants are not naturally occurring groups, as the setting is no longer natural. A quantitative research study can help pinpoint the exact conditions in which variables impact one another.

Examples of experimental quantitative design include:

• The effect of children’s Christmas gifts on a child’s dopamine (happiness) levels
• The effect of Black Friday sales on the success of the company
• The effect of product issues on the perceived reliability of the product

Quantitative research methods need to be carefully considered, as your data collection of a data type can be used to different effects. For example, statistics can be descriptive or correlational (or inferential). Descriptive statistics help us to summarize our data, while inferential statistics help infer conclusions about significant differences.

Advantages of quantitative research

• Easy to do : Doing quantitative research is more straightforward, as the results come in numerical format, which can be more easily interpreted.
• Less interpretation : Due to the factual nature of the results, you will be able to accept or reject your hypothesis based on the numerical data collected.
• Less bias : There are higher levels of control that can be applied to the research, so bias can be reduced , making your data more reliable and precise.

Disadvantages of quantitative research

• Can’t understand reasons: Quantitative research doesn’t always tell you the full story, meaning you won’t understand the context – or the why, of the data you see, why do you see the results you have uncovered?
• Useful for simpler situations: Quantitative research on its own is not great when dealing with complex issues. In these cases, quantitative research may not be enough.

How to use quantitative research to your business’s advantage

Quantitative research methods may help in areas such as:

• Identifying which advert or landing page performs better
• Identifying how satisfied your customers are
• How many customers are likely to recommend you
• Tracking how your brand ranks in awareness and customer purchase intent
• Learn what consumers are likely to buy from your brand.

6 steps to conducting good quantitative research

Businesses can benefit from quantitative research by using it to evaluate the impact of data types. There are several steps to this:

• Define your problem or interest area : What do you observe is happening and is it frequent? Identify the data type/s you’re observing.
• Create a hypothesis : Ask yourself what could be the causes for the situation with those data types.
• Plan your quantitative research : Use structured research instruments like surveys or polls to ask questions that test your hypothesis.
• Data Collection : Collect quantitative data and understand what your data types are telling you. Using data collected on different types over long time periods can give you information on patterns.
• Data analysis : Does your information support your hypothesis? (You may need to redo the research with other variables to see if the results improve)
• Effectively present data : Communicate the results in a clear and concise way to help other people understand the findings.

How Qualtrics products can enhance & simplify the quantitative research process

The Qualtrics XM system gives you an all-in-one, integrated solution to help you all the way through conducting quantitative research. From survey creation and data collection to statistical analysis and data reporting, it can help all your internal teams gain insights from your numerical data.

Quantitative methods are catered to your business through templates or advanced survey designs. While you can manually collect data and conduct data analysis in a spreadsheet program, this solution helps you automate the process of quantitative research, saving you time and administration work.

Using computational techniques helps you to avoid human errors, and participant results come in are already incorporated into the analysis in real-time.

Our key tools, Stats IQ™ and Driver IQ™ make analyzing numerical data easy and simple. Choose to highlight key findings based on variables or highlight statistically insignificant findings. The choice is yours.

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A Quick Guide to Quantitative Research in the Social Sciences

(12 reviews)

Christine Davies, Carmarthen, Wales

Copyright Year: 2020

Last Update: 2021

Publisher: University of Wales Trinity Saint David

Language: English

Formats Available

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Learn more about reviews.

Reviewed by Jennifer Taylor, Assistant Professor, Texas A&M University-Corpus Christi on 4/18/24

This resource is a quick guide to quantitative research in the social sciences and not a comprehensive resource. It provides a VERY general overview of quantitative research but offers a good starting place for students new to research. It... read more

Comprehensiveness rating: 4 see less

This resource is a quick guide to quantitative research in the social sciences and not a comprehensive resource. It provides a VERY general overview of quantitative research but offers a good starting place for students new to research. It offers links and references to additional resources that are more comprehensive in nature.

Content Accuracy rating: 4

The content is relatively accurate. The measurement scale section is very sparse. Not all types of research designs or statistical methods are included, but it is a guide, so details are meant to be limited.

Relevance/Longevity rating: 4

The examples were interesting and appropriate. The content is up to date and will be useful for several years.

Clarity rating: 5

The text was clearly written. Tables and figures are not referenced in the text, which would have been nice.

Consistency rating: 5

The framework is consistent across chapters with terminology clearly highlighted and defined.

Modularity rating: 5

The chapters are subdivided into section that can be divided and assigned as reading in a course. Most chapters are brief and concise, unless elaboration is necessary, such as with the data analysis chapter. Again, this is a guide and not a comprehensive text, so sections are shorter and don't always include every subtopic that may be considered.

Organization/Structure/Flow rating: 5

The guide is well organized. I appreciate that the topics are presented in a logical and clear manner. The topics are provided in an order consistent with traditional research methods.

Interface rating: 5

The interface was easy to use and navigate. The images were clear and easy to read.

Grammatical Errors rating: 5

I did not notice any grammatical errors.

Cultural Relevance rating: 5

The materials are not culturally insensitive or offensive in any way.

I teach a Marketing Research course to undergraduates. I would consider using some of the chapters or topics included, especially the overview of the research designs and the analysis of data section.

Reviewed by Tiffany Kindratt, Assistant Professor, University of Texas at Arlington on 3/9/24

The text provides a brief overview of quantitative research topics that is geared towards research in the fields of education, sociology, business, and nursing. The author acknowledges that the textbook is not a comprehensive resource but offers... read more

Comprehensiveness rating: 3 see less

The text provides a brief overview of quantitative research topics that is geared towards research in the fields of education, sociology, business, and nursing. The author acknowledges that the textbook is not a comprehensive resource but offers references to other resources that can be used to deepen the knowledge. The text does not include a glossary or index. The references in the figures for each chapter are not included in the reference section. It would be helpful to include those.

Overall, the text is accurate. For example, Figure 1 on page 6 provides a clear overview of the research process. It includes general definitions of primary and secondary research. It would be helpful to include more details to explain some of the examples before they are presented. For instance, the example on page 5 was unclear how it pertains to the literature review section.

In general, the text is relevant and up-to-date. The text includes many inferences of moving from qualitative to quantitative analysis. This was surprising to me as a quantitative researcher. The author mentions that moving from a qualitative to quantitative approach should only be done when needed. As a predominantly quantitative researcher, I would not advice those interested in transitioning to using a qualitative approach that qualitative research would enhance their research—not something that should only be done if you have to.

Clarity rating: 4

The text is written in a clear manner. It would be helpful to the reader if there was a description of the tables and figures in the text before they are presented.

Consistency rating: 4

The framework for each chapter and terminology used are consistent.

Modularity rating: 4

The text is clearly divided into sections within each chapter. Overall, the chapters are a similar brief length except for the chapter on data analysis, which is much more comprehensive than others.

Organization/Structure/Flow rating: 4

The topics in the text are presented in a clear and logical order. The order of the text follows the conventional research methodology in social sciences.

I did not encounter any interface issues when reviewing this text. All links worked and there were no distortions of the images or charts that may confuse the reader.

Grammatical Errors rating: 3

There are some grammatical/typographical errors throughout. Of note, for Section 5 in the table of contents. “The” should be capitalized to start the title. In the title for Table 3, the “t” in typical should be capitalized.

Cultural Relevance rating: 4

The examples are culturally relevant. The text is geared towards learners in the UK, but examples are relevant for use in other countries (i.e., United States). I did not see any examples that may be considered culturally insensitive or offensive in any way.

I teach a course on research methods in a Bachelor of Science in Public Health program. I would consider using some of the text, particularly in the analysis chapter to supplement the current textbook in the future.

Reviewed by Finn Bell, Assistant Professor, University of Michigan, Dearborn on 1/3/24

For it being a quick guide and only 26 pages, it is very comprehensive, but it does not include an index or glossary. read more

For it being a quick guide and only 26 pages, it is very comprehensive, but it does not include an index or glossary.

Content Accuracy rating: 5

As far as I can tell, the text is accurate, error-free and unbiased.

Relevance/Longevity rating: 5

This text is up-to-date, and given the content, unlikely to become obsolete any time soon.

The text is very clear and accessible.

The text is internally consistent.

Given how short the text is, it seems unnecessary to divide it into smaller readings, nonetheless, it is clearly labelled such that an instructor could do so.

The text is well-organized and brings readers through basic quantitative methods in a logical, clear fashion.

Easy to navigate. Only one table that is split between pages, but not in a way that is confusing.

There were no noticeable grammatical errors.

The examples in this book don't give enough information to rate this effectively.

This text is truly a very quick guide at only 26 double-spaced pages. Nonetheless, Davies packs a lot of information on the basics of quantitative research methods into this text, in an engaging way with many examples of the concepts presented. This guide is more of a brief how-to that takes readers as far as how to select statistical tests. While it would be impossible to fully learn quantitative research from such a short text, of course, this resource provides a great introduction, overview, and refresher for program evaluation courses.

Reviewed by Shari Fedorowicz, Adjunct Professor, Bridgewater State University on 12/16/22

The text is indeed a quick guide for utilizing quantitative research. Appropriate and effective examples and diagrams were used throughout the text. The author clearly differentiates between use of quantitative and qualitative research providing... read more

Comprehensiveness rating: 5 see less

The text is indeed a quick guide for utilizing quantitative research. Appropriate and effective examples and diagrams were used throughout the text. The author clearly differentiates between use of quantitative and qualitative research providing the reader with the ability to distinguish two terms that frequently get confused. In addition, links and outside resources are provided to deepen the understanding as an option for the reader. The use of these links, coupled with diagrams and examples make this text comprehensive.

The content is mostly accurate. Given that it is a quick guide, the author chose a good selection of which types of research designs to include. However, some are not provided. For example, correlational or cross-correlational research is omitted and is not discussed in Section 3, but is used as a statistical example in the last section.

Examples utilized were appropriate and associated with terms adding value to the learning. The tables that included differentiation between types of statistical tests along with a parametric/nonparametric table were useful and relevant.

The purpose to the text and how to use this guide book is stated clearly and is established up front. The author is also very clear regarding the skill level of the user. Adding to the clarity are the tables with terms, definitions, and examples to help the reader unpack the concepts. The content related to the terms was succinct, direct, and clear. Many times examples or figures were used to supplement the narrative.

The text is consistent throughout from contents to references. Within each section of the text, the introductory paragraph under each section provides a clear understanding regarding what will be discussed in each section. The layout is consistent for each section and easy to follow.

The contents are visible and address each section of the text. A total of seven sections, including a reference section, is in the contents. Each section is outlined by what will be discussed in the contents. In addition, within each section, a heading is provided to direct the reader to the subtopic under each section.

The text is well-organized and segues appropriately. I would have liked to have seen an introductory section giving a narrative overview of what is in each section. This would provide the reader with the ability to get a preliminary glimpse into each upcoming sections and topics that are covered.

The book was easy to navigate and well-organized. Examples are presented in one color, links in another and last, figures and tables. The visuals supplemented the reading and placed appropriately. This provides an opportunity for the reader to unpack the reading by use of visuals and examples.

No significant grammatical errors.

The text is not offensive or culturally insensitive. Examples were inclusive of various races, ethnicities, and backgrounds.

This quick guide is a beneficial text to assist in unpacking the learning related to quantitative statistics. I would use this book to complement my instruction and lessons, or use this book as a main text with supplemental statistical problems and formulas. References to statistical programs were appropriate and were useful. The text did exactly what was stated up front in that it is a direct guide to quantitative statistics. It is well-written and to the point with content areas easy to locate by topic.

Reviewed by Sarah Capello, Assistant Professor, Radford University on 1/18/22

The text claims to provide "quick and simple advice on quantitative aspects of research in social sciences," which it does. There is no index or glossary, although vocabulary words are bolded and defined throughout the text. read more

The text claims to provide "quick and simple advice on quantitative aspects of research in social sciences," which it does. There is no index or glossary, although vocabulary words are bolded and defined throughout the text.

The content is mostly accurate. I would have preferred a few nuances to be hashed out a bit further to avoid potential reader confusion or misunderstanding of the concepts presented.

The content is current; however, some of the references cited in the text are outdated. Newer editions of those texts exist.

The text is very accessible and readable for a variety of audiences. Key terms are well-defined.

There are no content discrepancies within the text. The author even uses similarly shaped graphics for recurring purposes throughout the text (e.g., arrow call outs for further reading, rectangle call outs for examples).

The content is chunked nicely by topics and sections. If it were used for a course, it would be easy to assign different sections of the text for homework, etc. without confusing the reader if the instructor chose to present the content in a different order.

The author follows the structure of the research process. The organization of the text is easy to follow and comprehend.

All of the supplementary images (e.g., tables and figures) were beneficial to the reader and enhanced the text.

There are no significant grammatical errors.

I did not find any culturally offensive or insensitive references in the text.

This text does the difficult job of introducing the complicated concepts and processes of quantitative research in a quick and easy reference guide fairly well. I would not depend solely on this text to teach students about quantitative research, but it could be a good jumping off point for those who have no prior knowledge on this subject or those who need a gentle introduction before diving in to more advanced and complex readings of quantitative research methods.

Reviewed by J. Marlie Henry, Adjunct Faculty, University of Saint Francis on 12/9/21

Considering the length of this guide, this does a good job of addressing major areas that typically need to be addressed. There is a contents section. The guide does seem to be organized accordingly with appropriate alignment and logical flow of... read more

Considering the length of this guide, this does a good job of addressing major areas that typically need to be addressed. There is a contents section. The guide does seem to be organized accordingly with appropriate alignment and logical flow of thought. There is no glossary but, for a guide of this length, a glossary does not seem like it would enhance the guide significantly.

The content is relatively accurate. Expanding the content a bit more or explaining that the methods and designs presented are not entirely inclusive would help. As there are different schools of thought regarding what should/should not be included in terms of these designs and methods, simply bringing attention to that and explaining a bit more would help.

Relevance/Longevity rating: 3

This content needs to be updated. Most of the sources cited are seven or more years old. Even more, it would be helpful to see more currently relevant examples. Some of the source authors such as Andy Field provide very interesting and dynamic instruction in general, but they have much more current information available.

The language used is clear and appropriate. Unnecessary jargon is not used. The intent is clear- to communicate simply in a straightforward manner.

The guide seems to be internally consistent in terms of terminology and framework. There do not seem to be issues in this area. Terminology is internally consistent.

For a guide of this length, the author structured this logically into sections. This guide could be adopted in whole or by section with limited modifications. Courses with fewer than seven modules could also logically group some of the sections.

This guide does present with logical organization. The topics presented are conceptually sequenced in a manner that helps learners build logically on prior conceptualization. This also provides a simple conceptual framework for instructors to guide learners through the process.

Interface rating: 4

The visuals themselves are simple, but they are clear and understandable without distracting the learner. The purpose is clear- that of learning rather than visuals for the sake of visuals. Likewise, navigation is clear and without issues beyond a broken link (the last source noted in the references).

This guide seems to be free of grammatical errors.

It would be interesting to see more cultural integration in a guide of this nature, but the guide is not culturally insensitive or offensive in any way. The language used seems to be consistent with APA's guidelines for unbiased language.

Reviewed by Heng Yu-Ku, Professor, University of Northern Colorado on 5/13/21

The text covers all areas and ideas appropriately and provides practical tables, charts, and examples throughout the text. I would suggest the author also provides a complete research proposal at the end of Section 3 (page 10) and a comprehensive... read more

The text covers all areas and ideas appropriately and provides practical tables, charts, and examples throughout the text. I would suggest the author also provides a complete research proposal at the end of Section 3 (page 10) and a comprehensive research study as an Appendix after section 7 (page 26) to help readers comprehend information better.

For the most part, the content is accurate and unbiased. However, the author only includes four types of research designs used on the social sciences that contain quantitative elements: 1. Mixed method, 2) Case study, 3) Quasi-experiment, and 3) Action research. I wonder why the correlational research is not included as another type of quantitative research design as it has been introduced and emphasized in section 6 by the author.

I believe the content is up-to-date and that necessary updates will be relatively easy and straightforward to implement.

The text is easy to read and provides adequate context for any technical terminology used. However, the author could provide more detailed information about estimating the minimum sample size but not just refer the readers to use the online sample calculators at a different website.

The text is internally consistent in terms of terminology and framework. The author provides the right amount of information with additional information or resources for the readers.

The text includes seven sections. Therefore, it is easier for the instructor to allocate or divide the content into different weeks of instruction within the course.

Yes, the topics in the text are presented in a logical and clear fashion. The author provides clear and precise terminologies, summarizes important content in Table or Figure forms, and offers examples in each section for readers to check their understanding.

The interface of the book is consistent and clear, and all the images and charts provided in the book are appropriate. However, I did encounter some navigation problems as a couple of links are not working or requires permission to access those (pages 10 and 27).

No grammatical errors were found.

No culturally incentive or offensive in its language and the examples provided were found.

As the book title stated, this book provides “A Quick Guide to Quantitative Research in Social Science. It offers easy-to-read information and introduces the readers to the research process, such as research questions, research paradigms, research process, research designs, research methods, data collection, data analysis, and data discussion. However, some links are not working or need permissions to access them (pages 10 and 27).

Reviewed by Hsiao-Chin Kuo, Assistant Professor, Northeastern Illinois University on 4/26/21, updated 4/28/21

As a quick guide, it covers basic concepts related to quantitative research. It starts with WHY quantitative research with regard to asking research questions and considering research paradigms, then provides an overview of research design and... read more

As a quick guide, it covers basic concepts related to quantitative research. It starts with WHY quantitative research with regard to asking research questions and considering research paradigms, then provides an overview of research design and process, discusses methods, data collection and analysis, and ends with writing a research report. It also identifies its target readers/users as those begins to explore quantitative research. It would be helpful to include more examples for readers/users who are new to quantitative research.

Its content is mostly accurate and no bias given its nature as a quick guide. Yet, it is also quite simplified, such as its explanations of mixed methods, case study, quasi-experimental research, and action research. It provides resources for extended reading, yet more recent works will be helpful.

The book is relevant given its nature as a quick guide. It would be helpful to provide more recent works in its resources for extended reading, such as the section for Survey Research (p. 12). It would also be helpful to include more information to introduce common tools and software for statistical analysis.

The book is written with clear and understandable language. Important terms and concepts are presented with plain explanations and examples. Figures and tables are also presented to support its clarity. For example, Table 4 (p. 20) gives an easy-to-follow overview of different statistical tests.

The framework is very consistent with key points, further explanations, examples, and resources for extended reading. The sample studies are presented following the layout of the content, such as research questions, design and methods, and analysis. These examples help reinforce readers' understanding of these common research elements.

The book is divided into seven chapters. Each chapter clearly discusses an aspect of quantitative research. It can be easily divided into modules for a class or for a theme in a research method class. Chapters are short and provides additional resources for extended reading.

The topics in the chapters are presented in a logical and clear structure. It is easy to follow to a degree. Though, it would be also helpful to include the chapter number and title in the header next to its page number.

The text is easy to navigate. Most of the figures and tables are displayed clearly. Yet, there are several sections with empty space that is a bit confusing in the beginning. Again, it can be helpful to include the chapter number/title next to its page number.

Grammatical Errors rating: 4

No major grammatical errors were found.

There are no cultural insensitivities noted.

Given the nature and purpose of this book, as a quick guide, it provides readers a quick reference for important concepts and terms related to quantitative research. Because this book is quite short (27 pages), it can be used as an overview/preview about quantitative research. Teacher's facilitation/input and extended readings will be needed for a deeper learning and discussion about aspects of quantitative research.

Reviewed by Yang Cheng, Assistant Professor, North Carolina State University on 1/6/21

It covers the most important topics such as research progress, resources, measurement, and analysis of the data. read more

It covers the most important topics such as research progress, resources, measurement, and analysis of the data.

The book accurately describes the types of research methods such as mixed-method, quasi-experiment, and case study. It talks about the research proposal and key differences between statistical analyses as well.

The book pinpointed the significance of running a quantitative research method and its relevance to the field of social science.

The book clearly tells us the differences between types of quantitative methods and the steps of running quantitative research for students.

The book is consistent in terms of terminologies such as research methods or types of statistical analysis.

It addresses the headlines and subheadlines very well and each subheading should be necessary for readers.

The book was organized very well to illustrate the topic of quantitative methods in the field of social science.

The pictures within the book could be further developed to describe the key concepts vividly.

The textbook contains no grammatical errors.

It is not culturally offensive in any way.

Overall, this is a simple and quick guide for this important topic. It should be valuable for undergraduate students who would like to learn more about research methods.

Reviewed by Pierre Lu, Associate Professor, University of Texas Rio Grande Valley on 11/20/20

As a quick guide to quantitative research in social sciences, the text covers most ideas and areas. read more

As a quick guide to quantitative research in social sciences, the text covers most ideas and areas.

Mostly accurate content.

As a quick guide, content is highly relevant.

Succinct and clear.

Internally, the text is consistent in terms of terminology used.

The text is easily and readily divisible into smaller sections that can be used as assignments.

I like that there are examples throughout the book.

Easy to read. No interface/ navigation problems.

No grammatical errors detected.

I am not aware of the culturally insensitive description. After all, this is a methodology book.

I think the book has potential to be adopted as a foundation for quantitative research courses, or as a review in the first weeks in advanced quantitative course.

Reviewed by Sarah Fischer, Assistant Professor, Marymount University on 7/31/20

It is meant to be an overview, but it incredibly condensed and spends almost no time on key elements of statistics (such as what makes research generalizable, or what leads to research NOT being generalizable). read more

It is meant to be an overview, but it incredibly condensed and spends almost no time on key elements of statistics (such as what makes research generalizable, or what leads to research NOT being generalizable).

Content Accuracy rating: 1

Contains VERY significant errors, such as saying that one can "accept" a hypothesis. (One of the key aspect of hypothesis testing is that one either rejects or fails to reject a hypothesis, but NEVER accepts a hypothesis.)

Very relevant to those experiencing the research process for the first time. However, it is written by someone working in the natural sciences but is a text for social sciences. This does not explain the errors, but does explain why sometimes the author assumes things about the readers ("hail from more subjectivist territory") that are likely not true.

Clarity rating: 3

Some statistical terminology not explained clearly (or accurately), although the author has made attempts to do both.

Very consistently laid out.

Chapters are very short yet also point readers to outside texts for additional information. Easy to follow.

Generally logically organized.

Easy to navigate, images clear. The additional sources included need to linked to.

Minor grammatical and usage errors throughout the text.

Makes efforts to be inclusive.

The idea of this book is strong--short guides like this are needed. However, this book would likely be strengthened by a revision to reduce inaccuracies and improve the definitions and technical explanations of statistical concepts. Since the book is specifically aimed at the social sciences, it would also improve the text to have more examples that are based in the social sciences (rather than the health sciences or the arts).

Reviewed by Michelle Page, Assistant Professor, Worcester State University on 5/30/20

This text is exactly intended to be what it says: A quick guide. A basic outline of quantitative research processes, akin to cliff notes. The content provides only the essentials of a research process and contains key terms. A student or new... read more

This text is exactly intended to be what it says: A quick guide. A basic outline of quantitative research processes, akin to cliff notes. The content provides only the essentials of a research process and contains key terms. A student or new researcher would not be able to use this as a stand alone guide for quantitative pursuits without having a supplemental text that explains the steps in the process more comprehensively. The introduction does provide this caveat.

Content Accuracy rating: 3

There are no biases or errors that could be distinguished; however, it’s simplicity in content, although accurate for an outline of process, may lack a conveyance of the deeper meanings behind the specific processes explained about qualitative research.

The content is outlined in traditional format to highlight quantitative considerations for formatting research foundational pieces. The resources/references used to point the reader to literature sources can be easily updated with future editions.

The jargon in the text is simple to follow and provides adequate context for its purpose. It is simplified for its intention as a guide which is appropriate.

Each section of the text follows a consistent flow. Explanation of the research content or concept is defined and then a connection to literature is provided to expand the readers understanding of the section’s content. Terminology is consistent with the qualitative process.

As an “outline” and guide, this text can be used to quickly identify the critical parts of the quantitative process. Although each section does not provide deeper content for meaningful use as a stand alone text, it’s utility would be excellent as a reference for a course and can be used as an content guide for specific research courses.

The text’s outline and content are aligned and are in a logical flow in terms of the research considerations for quantitative research.

The only issue that the format was not able to provide was linkable articles. These would have to be cut and pasted into a browser. Functional clickable links in a text are very successful at leading the reader to the supplemental material.

No grammatical errors were noted.

This is a very good outline “guide” to help a new or student researcher to demystify the quantitative process. A successful outline of any process helps to guide work in a logical and systematic way. I think this simple guide is a great adjunct to more substantial research context.

Table of Contents

• Section 1: What will this resource do for you?
• Section 2: Why are you thinking about numbers? A discussion of the research question and paradigms.
• Section 3: An overview of the Research Process and Research Designs
• Section 4: Quantitative Research Methods
• Section 5: the data obtained from quantitative research
• Section 6: Analysis of data
• Section 7: Discussing your Results

Ancillary Material

About the book.

This resource is intended as an easy-to-use guide for anyone who needs some quick and simple advice on quantitative aspects of research in social sciences, covering subjects such as education, sociology, business, nursing. If you area qualitative researcher who needs to venture into the world of numbers, or a student instructed to undertake a quantitative research project despite a hatred for maths, then this booklet should be a real help.

The booklet was amended in 2022 to take into account previous review comments.  

About the Contributors

Christine Davies , Ph.D

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A Comprehensive Guide to Quantitative Research: Types, Characteristics, Methods & Examples

Step into the fascinating world of quantitative research, where numbers reveal extraordinary insights!

By gathering and studying data in a systematic way, quantitative research empowers us to understand our ever-changing world better. It helps understand a problem or an already-formed hypothesis by generating numerical data. The results don’t end here, as you can process these numbers to get actionable insights that aid decision-making.

You can use quantitative research to quantify opinions, behaviors, attitudes, and other definitive variables related to the market, customers, competitors, etc. The research is conducted on a larger sample population to draw predictive, average, and pattern-based insights.

Here, we delve into the intricacies of this research methodology, exploring various quantitative methods, their advantages, and real-life examples that showcase their impact and relevance.

Ready to embark on a journey of discovery and knowledge? Let’s go!

What Is Quantitative Research?

Quantitative research is a method that uses numbers and statistics to test theories about customer attitudes and behaviors. It helps researchers gather and analyze data systematically to gain valuable insights and draw evidence-based conclusions about customer preferences and trends.

Researchers use online surveys , questionnaires , polls , and quizzes to question a large number of people to obtain measurable and bias-free data.

In technical terms, quantitative research is mainly concerned with discovering facts about social phenomena while assuming a fixed and measurable reality.

Offering numbers and stats-based insights, this research methodology is a crucial part of primary research and helps understand how well an organizational decision is going to work out.

Let’s consider an example.

Suppose your qualitative analysis shows that your customers are looking for social media-based customer support . In that case, quantitative analysis will help you see how many of your customers are looking for this support.

If 10% of your customers are looking for such a service, you might or might not consider offering this feature. But, if 40% of your regular customers are seeking support via social media, then it is something you just cannot overlook.

Characteristics of Quantitative Research

Quantitative research clarifies the fuzziness of research data from qualitative research analysis. With numerical insights, you can formulate a better and more profitable business decision.

Hence, quantitative research is more readily contestable, sharpens intelligent discussion, helps you see the rival hypotheses, and dynamically contributes to the research process.

Let us have a quick look at some of its characteristics.

• Measurable Variables

The data collection methods in quantitative research are structured and contain items requiring measurable variables, such as age, number of family members, salary range, highest education, etc.

These structured data collection methods comprise polls, surveys, questionnaires, etc., and may have questions like the ones shown in the following image:

As you can see, all the variables are measurable. This ensures that the research is in-depth and provides less erroneous data for reliable, actionable insights.

• Sample Size

No matter what data analysis methods for quantitative research are being used, the sample size is kept such that it represents the target market.

As the main aim of the research methodology is to get numerical insights, the sample size should be fairly large. Depending on the survey objective and scope, it might span hundreds of thousands of people.

• Normal Population Distribution

To maintain the reliability of a quantitative research methodology, we assume that the population distribution curve is normal.

This type of population distribution curve is preferred over a non-normal distribution as the sample size is large, and the characteristics of the sample vary with its size.

This requires adhering to the random sampling principle to avoid the researcher’s bias in result interpretation. Any bias can ruin the fairness of the entire process and defeats the purpose of research.

• Well-Structured Data Representation

Data analysis in quantitative research produces highly structured results and can form well-defined graphical representations. Some common examples include tables, figures, graphs, etc., that combine large blocks of data.

This way, you can discover hidden data trends, relationships, and differences among various measurable variables. This can help researchers understand the survey data and formulate actionable insights for decision-making.

• Predictive Outcomes

Quantitative analysis of data can also be used for estimations and prediction outcomes. You can construct if-then scenarios and analyze the data for the identification of any upcoming trends or events.

However, this requires advanced analytics and involves complex mathematical computations. So, it is mostly done via quantitative research tools that come with advanced analytics capabilities.

8 Best Practices to Conduct Quantitative Research

Here are some best practices to keep in mind while conducting quantitative research:

1. Define Research Objectives

There can be many ways to collect data via quantitative research methods that are chosen as per the research objective and scope. These methods allow you to build your own observations regarding any hypotheses – unknown, entirely new, or unexplained. 

You can hypothesize a proof and build a prediction of outcomes supporting the same. You can also create a detailed stepwise plan for data collection, analysis, and testing. 

Below, we explore quantitative research methods and discuss some examples to enhance your understanding of them.

2. Keep Your Questions Simple

The surveys are meant to reach people en-masse, and that includes a wide demographic range with recipients from all walks of life. Asking simple questions will ensure that they grasp what’s being asked easily.

Read More: Proven Tips to Avoid Leading and Loaded Questions in Your Survey

3. Develop a Solid Research Design

Choose an appropriate research design that aligns with your objectives, whether it’s experimental, quasi-experimental, or correlational. You also need to pay attention to the sample size and sampling technique such that it represents the target population accurately.

4. Use Reliable & Valid Instruments

It’s crucial to select or develop measurement instruments such as questionnaires, scales, or tests that have been validated and are reliable. Before proceeding with the main study, pilot-test these instruments on a small sample to assess their effectiveness and make any necessary improvements.

5. Ensure Data Quality

Implement data collection protocols to minimize errors and bias during data gathering. Double-check data entries and cleaning procedures to eliminate any inconsistencies or missing values that may affect the accuracy of your results. For instance, you might regularly cross-verify data entries to identify and correct any discrepancies.

6. Employ Appropriate Data Analysis Techniques

Select statistical methods that match the nature of your data and research questions. Whether it’s regression analysis, t-tests, ANOVA, or other techniques, using the right approach is important for drawing meaningful conclusions. Utilize software tools like SPSS or R for data analysis to ensure the accuracy and reproducibility of your findings.

7. Interpret Results Objectively

Present your findings in a clear and unbiased manner. Avoid making unwarranted causal claims, especially in correlational studies. Instead, focus on describing the relationships and patterns observed in your data.

8. Address Ethical Considerations

Prioritize ethical considerations throughout your research process. Obtain informed consent from participants, ensuring their voluntary participation and confidentiality of data. Comply with ethical guidelines and gain approval from a governing body if necessary.

Read More: How to Find Survey Participants & Respondents

Types of Quantitative Research Methods

Quantitative research is usually conducted using two methods. They are-

• Primary quantitative research methods
• Secondary quantitative research methods

1. Primary Methods

Primary quantitative research is the most popular way of conducting market research. The differentiating factor of this method is that the researcher relies on collecting data firsthand instead of relying on data collected from previous research.

There are multiple types of primary quantitative research. They can be distinguished based on three distinctive aspects, which are:

A. Techniques & Types of Studies:

• Survey Research

Surveys are the easiest, most common, and one of the most sought-after quantitative research techniques. The main aim of a survey is to widely gather and describe the characteristics of a target population or customers. Surveys are the foremost quantitative method preferred by both small and large organizations.

They help them understand their customers, products, and other brand offerings in a proper manner.

Surveys can be conducted using various methods, such as online polls, web-based surveys, paper questionnaires, phone calls, or face-to-face interviews. Survey research allows organizations to understand customer opinions, preferences, and behavior, making it crucial for market research and decision-making.

You can watch this quick video to learn more about creating surveys.

Surveys are of two types:

• Cross-Sectional Surveys Cross-sectional surveys are used to collect data from a sample of the target population at a specific point in time. Researchers evaluate various variables simultaneously to understand the relationships and patterns within the data.
• Cross-sectional surveys are popular in retail, small and medium-sized enterprises (SMEs), and healthcare industries, where they assess customer satisfaction, market trends, and product feedback.
• Longitudinal Surveys Longitudinal surveys are conducted over an extended period, observing changes in respondent behavior and thought processes.
• Researchers gather data from the same sample multiple times, enabling them to study trends and developments over time. These surveys are valuable in fields such as medicine, applied sciences, and market trend analysis.

Surveys can be distributed via various channels. Some of the most popular ones are listed below:

• Email: Sending surveys via email is a popular and effective method. People recognize your brand, leading to a higher response rate. With ProProfs Survey Maker’s in-mail survey-filling feature, you can easily send out and collect survey responses.
• Embed on a website: Boost your response rate by embedding the survey on your website. When visitors are already engaged with your brand, they are more likely to take the survey.
• Social media: Take advantage of social media platforms to distribute your survey. People familiar with your brand are likely to respond, increasing your response numbers.
• QR codes: QR codes store your survey’s URL, and you can print or publish these codes in magazines, signs, business cards, or any object to make it easy for people to access your survey.
• SMS survey: Collect a high number of responses quickly with SMS surveys. It’s a time-effective way to reach your target audience.

Read More: 24 Different Types of Survey Methods With Examples

2. Correlational Research:

Correlational research aims to establish relationships between two or more variables.

Researchers use statistical analysis to identify patterns and trends in the data, but it does not determine causality between the variables. This method helps understand how changes in one variable may impact another.

Examples of correlational research questions include studying the relationship between stress and depression, fame and money, or classroom activities and student performance.

3. Causal-Comparative Research:

Causal-comparative research, also known as quasi-experimental research, seeks to determine cause-and-effect relationships between variables.

Researchers analyze how an independent variable influences a dependent variable, but they do not manipulate the independent variable. Instead, they observe and compare different groups to draw conclusions.

Causal-comparative research is useful in situations where it’s not ethical or feasible to conduct true experiments.

Examples of questions for this type of research include analyzing the effect of training programs on employee performance, studying the influence of customer support on client retention, investigating the impact of supply chain efficiency on cost reduction, etc.

4. Experimental Research:

Experimental research is based on testing theories to validate or disprove them. Researchers conduct experiments and manipulate variables to observe their impact on the outcomes.

This type of research is prevalent in natural and social sciences, and it is a powerful method to establish cause-and-effect relationships. By randomly assigning participants to experimental and control groups, researchers can draw more confident conclusions.

Examples of experimental research include studying the effectiveness of a new drug, the impact of teaching methods on student performance, or the outcomes of a marketing campaign.

B. Data collection methodologies

After defining research objectives, the next significant step in primary quantitative research is data collection. This involves using two main methods: sampling and conducting surveys or polls.

Sampling methods:

In quantitative research, there are two primary sampling methods: Probability and Non-probability sampling.

Probability Sampling

In probability sampling, researchers use the concept of probability to create samples from a population. This method ensures that every individual in the target audience has an equal chance of being selected for the sample.

There are four main types of probability sampling:

• Simple random sampling: Here, the elements or participants of a sample are selected randomly, and this technique is used in studies that are conducted over considerably large audiences. It works well for large target populations.
• Stratified random sampling: In this method, the entire population is divided into strata or groups, and the sample members get chosen randomly from these strata only. It is always ensured that different segregated strata do not overlap with each other.
• Cluster sampling: Here, researchers divide the population into clusters, often based on geography or demographics. Then, random clusters are selected for the sample.
• Systematic sampling: In this method, only the starting point of the sample is randomly chosen. All the other participants are chosen using a fixed interval. Researchers calculate this interval by dividing the size of the study population by the target sample size.

Non-probability Sampling

Non-probability sampling is a method where the researcher’s knowledge and experience guide the selection of samples. This approach doesn’t give all members of the target population an equal chance of being included in the sample.

There are five non-probability sampling models:

• Convenience sampling: The elements or participants are chosen on the basis of their nearness to the researcher. The people in close proximity can be studied and analyzed easily and quickly, as there is no other selection criterion involved. Researchers simply choose samples based on what is most convenient for them.
• Consecutive sampling: Similar to convenience sampling, researchers select samples one after another over a significant period. They can opt for a single participant or a group of samples to conduct quantitative research in a consecutive manner for a significant period of time. Once this is over, they can conduct the research from the start.
• Quota sampling: With quota sampling, researchers use their understanding of target traits and personalities to form groups (strata). They then choose samples from each stratum based on their own judgment.
• Snowball sampling: This method is used where the target audiences are difficult to contact and interviewed for data collection. Researchers start with a few participants and then ask them to refer others, creating a snowball effect.
• Judgmental sampling: In judgmental sampling, researchers rely solely on their experience and research skills to handpick samples that they believe will be most relevant to the study.

Read More: Data Collection Methods: Definition, Types & Examples

C. Data analysis techniques

To analyze the quantitative data accurately, you’ll need to use specific statistical methods such as:

• SWOT Analysis: This stands for Strengths, Weaknesses, Opportunities, and Threats analysis. Organizations use SWOT analysis to evaluate their performance internally and externally. It helps develop effective improvement strategies.
• Conjoint Analysis: This market research method uncovers how individuals make complex purchasing decisions. It involves considering trade-offs in their daily activities when choosing from a list of product/service options.
• Cross-tabulation: A preliminary statistical market analysis method that reveals relationships, patterns, and trends within various research study parameters.
• TURF Analysis: Short for Totally Unduplicated Reach and Frequency Analysis, this method helps analyze the reach and frequency of favorable communication sources. It provides insights into the potential of a target market.
• By using these statistical techniques and inferential statistics methods like confidence intervals and margin of error, you can draw meaningful insights from your primary quantitative research that you can use in making informed decisions.

II. Secondary Quantitative Research Methods

• Secondary quantitative research, also known as desk research, is a valuable method that uses existing data, called secondary data.
• Instead of collecting new data, researchers analyze and combine already available information to enhance their research. This approach involves gathering quantitative data from various sources such as the internet, government databases, libraries, and research reports.
• Secondary quantitative research plays a crucial role in validating data collected through primary quantitative research. It helps reinforce or challenge existing findings.

Here are five commonly used secondary quantitative research methods:

A. Data Available on the Internet:

The Internet has become a vast repository of data, making it easier for researchers to access a wealth of information. Online databases, websites, and research repositories provide valuable quantitative data for researchers to analyze and validate their primary research findings.

B. Government and Non-Government Sources:

Government agencies and non-government organizations often conduct extensive research and publish reports. These reports cover a wide range of topics, providing researchers with reliable and comprehensive data for quantitative analysis.

C. Public Libraries:

While less commonly used in the digital age, public libraries still hold valuable research reports, historical data, and publications that can contribute to quantitative research.

D. Educational Institutions:

Educational institutions frequently conduct research on various subjects. Their research reports and publications can serve as valuable sources of information for researchers, validating and supporting primary quantitative research outcomes.

E. Commercial Information Sources:

Commercial sources such as local newspapers, journals, magazines, and media outlets often publish relevant data on economic trends, market research, and demographic analyses. Researchers can access this data to supplement their own findings and draw better conclusions.

Advantages of Quantitative Research Methods

Quantitative research data is often standardized and can be easily used to generalize findings for making crucial business decisions and uncover insights to supplement the qualitative research findings.

Here are some core benefits this research methodology offers.

Direct Result Comparison

As the studies can be replicated for different cultural settings and different times, even with different groups of participants, they tend to be extremely useful. Researchers can compare the results of different studies in a statistical manner and arrive at comprehensive conclusions for a broader understanding.

Replication

Researchers can repeat the study by using standardized data collection protocols over well-structured data sets. They can also apply tangible definitions of abstract concepts to arrive at different conclusions for similar research objectives with minor variations.

Large Samples

As the research data comes from large samples, the researchers can process and analyze the data via highly reliable and consistent analysis procedures. They can arrive at well-defined conclusions that can be used to make the primary research more thorough and reliable.

Hypothesis Testing

This research methodology follows standardized and established hypothesis testing procedures. So, you have to be careful while reporting and analyzing your research data , and the overall quality of results gets improved.

Proven Examples of Quantitative Research Methods

Below, we discuss two excellent examples of quantitative research methods that were used by highly distinguished business and consulting organizations. Both examples show how different types of analysis can be performed with qualitative approaches and how the analysis is done once the data is collected.

1. STEP Project Global Consortium / KPMG 2019 Global Family Business survey

This research utilized quantitative methods to identify ways that kept the family businesses sustainably profitable with time.

The study also identified the ways in which the family business behavior changed with demographic changes and had “why” and “how” questions. Their qualitative research methods allowed the KPMG team to dig deeper into the mindsets and perspectives of the business owners and uncover unexpected research avenues as well.

It was a joint effort in which STEP Project Global Consortium collected 26 cases, and KPMG collected 11 cases.

The research reached the stage of data analysis in 2020, and the analysis process spanned over 4 stages.

The results, which were also the reasons why family businesses tend to lose their strength with time, were found to be:

• Family governance
• Family business legacy

2. EY Seren Teams Research 2020

This is yet another commendable example of qualitative research where the EY Seren Team digs into the unexplored depths of human behavior and how it affected their brand or service expectations.

The research was done across 200+ sources and involved in-depth virtual interviews with people in their homes, exploring their current needs and wishes. It also involved diary studies across the entire UK customer base to analyze human behavior changes and patterns.

The study also included interviews with professionals and design leaders from a wide range of industries to explore how COVID-19 transformed their industries. Finally, quantitative surveys were conducted to gain insights into the EY community after every 15 days.

The insights and results were:

• A culture of fear, daily resilience, and hopes for a better world and a better life – these were the macro trends.
• People felt massive digitization to be a resourceful yet demanding aspect as they have to adapt every day.
• Some people wished to have a new world with lots of possibilities, and some were looking for a new purpose.

Enhance Your Quantitative Research With Cutting-Edge Software

While no single research methodology can produce 100% reliable results, you can always opt for a hybrid research method by opting for the methods that are most relevant to your objective.

This understanding comes gradually as you learn how to implement the correct combination of qualitative and quantitative research methods for your research projects. For the best results, we recommend investing in smart, efficient, and scalable research tools that come with delightful reporting and advanced analytics to make every research initiative a success.

These software tools, such as ProProfs Survey Maker, come with pre-built survey templates and question libraries and allow you to create a high-converting survey in just a few minutes.

So, choose the best research partner, create the right research plan, and gather insights that drive sustainable growth for your business.

About the author

Emma David is a seasoned market research professional with 8+ years of experience. Having kick-started her journey in research, she has developed rich expertise in employee engagement, survey creation and administration, and data management. Emma believes in the power of data to shape business performance positively. She continues to help brands and businesses make strategic decisions and improve their market standing through her understanding of research methodologies.

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Research Methodologies

Quantitative research methodologies.

• Qualitative Research Methodologies
• Systematic Reviews
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What is quantitative research.

Quantitative methodologies use statistics to analyze numerical data gathered by researchers to answer their research questions. Quantitative methods can be used to answer questions such as:

• What are the relationships between two or more variables? 
• What factors are at play in an environment that might affect the behavior or development of the organisms in that environment?

Quantitative methods can also be used to test hypotheses by conducting quasi-experimental studies or designing experiments.

Independent and Dependent Variables

In quantitative research, a variable is something (an intervention technique, a pharmaceutical, a temperature, etc.) that changes. There are two kinds of variables:  independent variables and dependent variables . In the simplest terms, the independent variable is whatever the researchers are using to attempt to make a change in their dependent variable.

* This is a real, repeatable experiment you can try on your plants.

Correlational

Researchers will compare two sets of numbers to try and identify a relationship (if any) between two things.

• Köse S., & Murat, M. (2021). Examination of the relationship between smartphone addiction and cyberchondria in adolescents. Archives of Psychiatric Nursing, 35(6): 563-570.
• Pilger et al. (2021). Spiritual well-being, religious/spiritual coping and quality of life among the elderly undergoing hemodialysis: a correlational study. Journal of Religion, Spirituality & Aging, 33(1): 2-15.

Descriptive

Researchers will attempt to quantify a variety of factors at play as they study a particular type of phenomenon or action. For example, researchers might use a descriptive methodology to understand the effects of climate change on the life cycle of a plant or animal. 

• Lakshmi, E. (2021). Food consumption pattern and body mass index of adolescents: A descriptive study. International Journal of Nutrition, Pharmacology, Neurological Diseases, 11(4), 293–297.
• Lin, J., Singh, S., Sha, L., Tan, W., Lang, D., Gašević, D., & Chen, G. (2022). Is it a good move? Mining effective tutoring strategies from human–human tutorial dialogues. Future Generation Computer Systems, 127, 194–207.

Experimental

To understand the effects of a variable, researchers will design an experiment where they can control as many factors as possible. This can involve creating control and experimental groups. The experimental group will be exposed to the variable to study its effects. The control group provides data about what happens when the variable is absent. For example, in a study about online teaching, the control group might receive traditional face-to-face instruction while the experimental group would receive their instruction virtually. 

• Jinzhang Jia, Yinuo Chen, Guangbo Che, Jinchao Zhu, Fengxiao Wang, & Peng Jia. (2021). Experimental study on the explosion characteristics of hydrogen-methane premixed gas in complex pipe networks. Scientific Reports, 11(1), 1–11.
• Sasaki, R. et al. (2021). Effects of cryotherapy applied at different temperatures on inflammatory pain during the acute phase of arthritis in rats. Physical Therapy, 101(2), 1–9.

Quasi-Experimental/Quasi-Comparative

Researchers will attempt to determine what (if any) effect a variable can have. These studies may have multiple independent variables (causes) and multiple dependent variables (effects), but this can complicate researchers' efforts to find out if A can cause B or if X, Y,  and  Z are also playing a role.

• Jafari, A., Alami, A., Charoghchian, E., Delshad Noghabi, A., & Nejatian, M. (2021). The impact of effective communication skills training on the status of marital burnout among married women. BMC Women’s Health, 21(1), 1-10.
• Phillips, S. W., Kim, D.-Y., Sobol, J. J., & Gayadeen, S. M. (2021). Total recall?: A quasi-experimental study of officer’s recollection in shoot - don’t shoot simulators. Police Practice and Research, 22(3), 1229–1240.

Surveys can be considered a quantitative methodology if the researchers require their respondents to choose from pre-determined responses. 

• Harries et al. (2021). Effects of the COVID-19 pandemic on medical students: A multicenter quantitative study. BMC Medical Education, 21(14), 1-8.
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Quantitative Research: Examples of Research Questions and Solutions

Are you ready to embark on a journey into the world of quantitative research? Whether you’re a seasoned researcher or just beginning your academic journey, understanding how to formulate effective research questions is essential for conducting meaningful studies. In this blog post, we’ll explore examples of quantitative research questions across various disciplines and discuss how StatsCamp.org courses can provide the tools and support you need to overcome any challenges you may encounter along the way.

Understanding Quantitative Research Questions

Quantitative research involves collecting and analyzing numerical data to answer research questions and test hypotheses. These questions typically seek to understand the relationships between variables, predict outcomes, or compare groups. Let’s explore some examples of quantitative research questions across different fields:

• What is the relationship between class size and student academic performance?
• Does the use of technology in the classroom improve learning outcomes?
• How does parental involvement affect student achievement?
• What is the effect of a new drug treatment on reducing blood pressure?
• Is there a correlation between physical activity levels and the risk of cardiovascular disease?
• How does socioeconomic status influence access to healthcare services?
• What factors influence consumer purchasing behavior?
• Is there a relationship between advertising expenditure and sales revenue?
• How do demographic variables affect brand loyalty?

Stats Camp: Your Solution to Mastering Quantitative Research Methodologies

At StatsCamp.org, we understand that navigating the complexities of quantitative research can be daunting. That’s why we offer a range of courses designed to equip you with the knowledge and skills you need to excel in your research endeavors. Whether you’re interested in learning about regression analysis, experimental design, or structural equation modeling, our experienced instructors are here to guide you every step of the way.

Bringing Your Own Data

One of the unique features of StatsCamp.org is the opportunity to bring your own data to the learning process. Our instructors provide personalized guidance and support to help you analyze your data effectively and overcome any roadblocks you may encounter. Whether you’re struggling with data cleaning, model specification, or interpretation of results, our team is here to help you succeed.

Courses Offered at StatsCamp.org

• Latent Profile Analysis Course : Learn how to identify subgroups, or profiles, within a heterogeneous population based on patterns of responses to multiple observed variables.
• Bayesian Statistics Course : A comprehensive introduction to Bayesian data analysis, a powerful statistical approach for inference and decision-making. Through a series of engaging lectures and hands-on exercises, participants will learn how to apply Bayesian methods to a wide range of research questions and data types.
• Structural Equation Modeling (SEM) Course : Dive into advanced statistical techniques for modeling complex relationships among variables.
• Multilevel Modeling Course : A in-depth exploration of this advanced statistical technique, designed to analyze data with nested structures or hierarchies. Whether you’re studying individuals within groups, schools within districts, or any other nested data structure, multilevel modeling provides the tools to account for the dependencies inherent in such data.

As you embark on your journey into quantitative research, remember that StatsCamp.org is here to support you every step of the way. Whether you’re formulating research questions, analyzing data, or interpreting results, our courses provide the knowledge and expertise you need to succeed. Join us today and unlock the power of quantitative research!

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Home » 500+ Quantitative Research Titles and Topics

500+ Quantitative Research Titles and Topics

Table of Contents

Quantitative research involves collecting and analyzing numerical data to identify patterns, trends, and relationships among variables. This method is widely used in social sciences, psychology , economics , and other fields where researchers aim to understand human behavior and phenomena through statistical analysis. If you are looking for a quantitative research topic, there are numerous areas to explore, from analyzing data on a specific population to studying the effects of a particular intervention or treatment. In this post, we will provide some ideas for quantitative research topics that may inspire you and help you narrow down your interests.

Quantitative Research Titles

Quantitative Research Titles are as follows:

Business and Economics

• “Statistical Analysis of Supply Chain Disruptions on Retail Sales”
• “Quantitative Examination of Consumer Loyalty Programs in the Fast Food Industry”
• “Predicting Stock Market Trends Using Machine Learning Algorithms”
• “Influence of Workplace Environment on Employee Productivity: A Quantitative Study”
• “Impact of Economic Policies on Small Businesses: A Regression Analysis”
• “Customer Satisfaction and Profit Margins: A Quantitative Correlation Study”
• “Analyzing the Role of Marketing in Brand Recognition: A Statistical Overview”
• “Quantitative Effects of Corporate Social Responsibility on Consumer Trust”
• “Price Elasticity of Demand for Luxury Goods: A Case Study”
• “The Relationship Between Fiscal Policy and Inflation Rates: A Time-Series Analysis”
• “Factors Influencing E-commerce Conversion Rates: A Quantitative Exploration”
• “Examining the Correlation Between Interest Rates and Consumer Spending”
• “Standardized Testing and Academic Performance: A Quantitative Evaluation”
• “Teaching Strategies and Student Learning Outcomes in Secondary Schools: A Quantitative Study”
• “The Relationship Between Extracurricular Activities and Academic Success”
• “Influence of Parental Involvement on Children’s Educational Achievements”
• “Digital Literacy in Primary Schools: A Quantitative Assessment”
• “Learning Outcomes in Blended vs. Traditional Classrooms: A Comparative Analysis”
• “Correlation Between Teacher Experience and Student Success Rates”
• “Analyzing the Impact of Classroom Technology on Reading Comprehension”
• “Gender Differences in STEM Fields: A Quantitative Analysis of Enrollment Data”
• “The Relationship Between Homework Load and Academic Burnout”
• “Assessment of Special Education Programs in Public Schools”
• “Role of Peer Tutoring in Improving Academic Performance: A Quantitative Study”

Medicine and Health Sciences

• “The Impact of Sleep Duration on Cardiovascular Health: A Cross-sectional Study”
• “Analyzing the Efficacy of Various Antidepressants: A Meta-Analysis”
• “Patient Satisfaction in Telehealth Services: A Quantitative Assessment”
• “Dietary Habits and Incidence of Heart Disease: A Quantitative Review”
• “Correlations Between Stress Levels and Immune System Functioning”
• “Smoking and Lung Function: A Quantitative Analysis”
• “Influence of Physical Activity on Mental Health in Older Adults”
• “Antibiotic Resistance Patterns in Community Hospitals: A Quantitative Study”
• “The Efficacy of Vaccination Programs in Controlling Disease Spread: A Time-Series Analysis”
• “Role of Social Determinants in Health Outcomes: A Quantitative Exploration”
• “Impact of Hospital Design on Patient Recovery Rates”
• “Quantitative Analysis of Dietary Choices and Obesity Rates in Children”

Social Sciences

• “Examining Social Inequality through Wage Distribution: A Quantitative Study”
• “Impact of Parental Divorce on Child Development: A Longitudinal Study”
• “Social Media and its Effect on Political Polarization: A Quantitative Analysis”
• “The Relationship Between Religion and Social Attitudes: A Statistical Overview”
• “Influence of Socioeconomic Status on Educational Achievement”
• “Quantifying the Effects of Community Programs on Crime Reduction”
• “Public Opinion and Immigration Policies: A Quantitative Exploration”
• “Analyzing the Gender Representation in Political Offices: A Quantitative Study”
• “Impact of Mass Media on Public Opinion: A Regression Analysis”
• “Influence of Urban Design on Social Interactions in Communities”
• “The Role of Social Support in Mental Health Outcomes: A Quantitative Analysis”
• “Examining the Relationship Between Substance Abuse and Employment Status”

Engineering and Technology

• “Performance Evaluation of Different Machine Learning Algorithms in Autonomous Vehicles”
• “Material Science: A Quantitative Analysis of Stress-Strain Properties in Various Alloys”
• “Impacts of Data Center Cooling Solutions on Energy Consumption”
• “Analyzing the Reliability of Renewable Energy Sources in Grid Management”
• “Optimization of 5G Network Performance: A Quantitative Assessment”
• “Quantifying the Effects of Aerodynamics on Fuel Efficiency in Commercial Airplanes”
• “The Relationship Between Software Complexity and Bug Frequency”
• “Machine Learning in Predictive Maintenance: A Quantitative Analysis”
• “Wearable Technologies and their Impact on Healthcare Monitoring”
• “Quantitative Assessment of Cybersecurity Measures in Financial Institutions”
• “Analysis of Noise Pollution from Urban Transportation Systems”
• “The Influence of Architectural Design on Energy Efficiency in Buildings”

Quantitative Research Topics

Quantitative Research Topics are as follows:

• The effects of social media on self-esteem among teenagers.
• A comparative study of academic achievement among students of single-sex and co-educational schools.
• The impact of gender on leadership styles in the workplace.
• The correlation between parental involvement and academic performance of students.
• The effect of mindfulness meditation on stress levels in college students.
• The relationship between employee motivation and job satisfaction.
• The effectiveness of online learning compared to traditional classroom learning.
• The correlation between sleep duration and academic performance among college students.
• The impact of exercise on mental health among adults.
• The relationship between social support and psychological well-being among cancer patients.
• The effect of caffeine consumption on sleep quality.
• A comparative study of the effectiveness of cognitive-behavioral therapy and pharmacotherapy in treating depression.
• The relationship between physical attractiveness and job opportunities.
• The correlation between smartphone addiction and academic performance among high school students.
• The impact of music on memory recall among adults.
• The effectiveness of parental control software in limiting children’s online activity.
• The relationship between social media use and body image dissatisfaction among young adults.
• The correlation between academic achievement and parental involvement among minority students.
• The impact of early childhood education on academic performance in later years.
• The effectiveness of employee training and development programs in improving organizational performance.
• The relationship between socioeconomic status and access to healthcare services.
• The correlation between social support and academic achievement among college students.
• The impact of technology on communication skills among children.
• The effectiveness of mindfulness-based stress reduction programs in reducing symptoms of anxiety and depression.
• The relationship between employee turnover and organizational culture.
• The correlation between job satisfaction and employee engagement.
• The impact of video game violence on aggressive behavior among children.
• The effectiveness of nutritional education in promoting healthy eating habits among adolescents.
• The relationship between bullying and academic performance among middle school students.
• The correlation between teacher expectations and student achievement.
• The impact of gender stereotypes on career choices among high school students.
• The effectiveness of anger management programs in reducing violent behavior.
• The relationship between social support and recovery from substance abuse.
• The correlation between parent-child communication and adolescent drug use.
• The impact of technology on family relationships.
• The effectiveness of smoking cessation programs in promoting long-term abstinence.
• The relationship between personality traits and academic achievement.
• The correlation between stress and job performance among healthcare professionals.
• The impact of online privacy concerns on social media use.
• The effectiveness of cognitive-behavioral therapy in treating anxiety disorders.
• The relationship between teacher feedback and student motivation.
• The correlation between physical activity and academic performance among elementary school students.
• The impact of parental divorce on academic achievement among children.
• The effectiveness of diversity training in improving workplace relationships.
• The relationship between childhood trauma and adult mental health.
• The correlation between parental involvement and substance abuse among adolescents.
• The impact of social media use on romantic relationships among young adults.
• The effectiveness of assertiveness training in improving communication skills.
• The relationship between parental expectations and academic achievement among high school students.
• The correlation between sleep quality and mood among adults.
• The impact of video game addiction on academic performance among college students.
• The effectiveness of group therapy in treating eating disorders.
• The relationship between job stress and job performance among teachers.
• The correlation between mindfulness and emotional regulation.
• The impact of social media use on self-esteem among college students.
• The effectiveness of parent-teacher communication in promoting academic achievement among elementary school students.
• The impact of renewable energy policies on carbon emissions
• The relationship between employee motivation and job performance
• The effectiveness of psychotherapy in treating eating disorders
• The correlation between physical activity and cognitive function in older adults
• The effect of childhood poverty on adult health outcomes
• The impact of urbanization on biodiversity conservation
• The relationship between work-life balance and employee job satisfaction
• The effectiveness of eye movement desensitization and reprocessing (EMDR) in treating trauma
• The correlation between parenting styles and child behavior
• The effect of social media on political polarization
• The impact of foreign aid on economic development
• The relationship between workplace diversity and organizational performance
• The effectiveness of dialectical behavior therapy in treating borderline personality disorder
• The correlation between childhood abuse and adult mental health outcomes
• The effect of sleep deprivation on cognitive function
• The impact of trade policies on international trade and economic growth
• The relationship between employee engagement and organizational commitment
• The effectiveness of cognitive therapy in treating postpartum depression
• The correlation between family meals and child obesity rates
• The effect of parental involvement in sports on child athletic performance
• The impact of social entrepreneurship on sustainable development
• The relationship between emotional labor and job burnout
• The effectiveness of art therapy in treating dementia
• The correlation between social media use and academic procrastination
• The effect of poverty on childhood educational attainment
• The impact of urban green spaces on mental health
• The relationship between job insecurity and employee well-being
• The effectiveness of virtual reality exposure therapy in treating anxiety disorders
• The correlation between childhood trauma and substance abuse
• The effect of screen time on children’s social skills
• The impact of trade unions on employee job satisfaction
• The relationship between cultural intelligence and cross-cultural communication
• The effectiveness of acceptance and commitment therapy in treating chronic pain
• The correlation between childhood obesity and adult health outcomes
• The effect of gender diversity on corporate performance
• The impact of environmental regulations on industry competitiveness.
• The impact of renewable energy policies on greenhouse gas emissions
• The relationship between workplace diversity and team performance
• The effectiveness of group therapy in treating substance abuse
• The correlation between parental involvement and social skills in early childhood
• The effect of technology use on sleep patterns
• The impact of government regulations on small business growth
• The relationship between job satisfaction and employee turnover
• The effectiveness of virtual reality therapy in treating anxiety disorders
• The correlation between parental involvement and academic motivation in adolescents
• The effect of social media on political engagement
• The impact of urbanization on mental health
• The relationship between corporate social responsibility and consumer trust
• The correlation between early childhood education and social-emotional development
• The effect of screen time on cognitive development in young children
• The impact of trade policies on global economic growth
• The relationship between workplace diversity and innovation
• The effectiveness of family therapy in treating eating disorders
• The correlation between parental involvement and college persistence
• The effect of social media on body image and self-esteem
• The impact of environmental regulations on business competitiveness
• The relationship between job autonomy and job satisfaction
• The effectiveness of virtual reality therapy in treating phobias
• The correlation between parental involvement and academic achievement in college
• The effect of social media on sleep quality
• The impact of immigration policies on social integration
• The relationship between workplace diversity and employee well-being
• The effectiveness of psychodynamic therapy in treating personality disorders
• The correlation between early childhood education and executive function skills
• The effect of parental involvement on STEM education outcomes
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• The impact of urbanization on public safety services.

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• Methodology
• Qualitative vs Quantitative Research | Examples & Methods

Qualitative vs Quantitative Research | Examples & Methods

Published on 4 April 2022 by Raimo Streefkerk . Revised on 8 May 2023.

When collecting and analysing data, quantitative research deals with numbers and statistics, while qualitative research  deals with words and meanings. Both are important for gaining different kinds of knowledge.

Common quantitative methods include experiments, observations recorded as numbers, and surveys with closed-ended questions. Qualitative research Qualitative research is expressed in words . It is used to understand concepts, thoughts or experiences. This type of research enables you to gather in-depth insights on topics that are not well understood.

Table of contents

The differences between quantitative and qualitative research, data collection methods, when to use qualitative vs quantitative research, how to analyse qualitative and quantitative data, frequently asked questions about qualitative and quantitative research.

Quantitative and qualitative research use different research methods to collect and analyse data, and they allow you to answer different kinds of research questions.

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Quantitative and qualitative data can be collected using various methods. It is important to use a data collection method that will help answer your research question(s).

Many data collection methods can be either qualitative or quantitative. For example, in surveys, observations or case studies , your data can be represented as numbers (e.g. using rating scales or counting frequencies) or as words (e.g. with open-ended questions or descriptions of what you observe).

However, some methods are more commonly used in one type or the other.

Quantitative data collection methods

• Surveys :  List of closed or multiple choice questions that is distributed to a sample (online, in person, or over the phone).
• Experiments : Situation in which variables are controlled and manipulated to establish cause-and-effect relationships.
• Observations: Observing subjects in a natural environment where variables can’t be controlled.

Qualitative data collection methods

• Interviews : Asking open-ended questions verbally to respondents.
• Focus groups: Discussion among a group of people about a topic to gather opinions that can be used for further research.
• Ethnography : Participating in a community or organisation for an extended period of time to closely observe culture and behavior.
• Literature review : Survey of published works by other authors.

A rule of thumb for deciding whether to use qualitative or quantitative data is:

• Use quantitative research if you want to confirm or test something (a theory or hypothesis)
• Use qualitative research if you want to understand something (concepts, thoughts, experiences)

For most research topics you can choose a qualitative, quantitative or mixed methods approach . Which type you choose depends on, among other things, whether you’re taking an inductive vs deductive research approach ; your research question(s) ; whether you’re doing experimental , correlational , or descriptive research ; and practical considerations such as time, money, availability of data, and access to respondents.

Quantitative research approach

You survey 300 students at your university and ask them questions such as: ‘on a scale from 1-5, how satisfied are your with your professors?’

You can perform statistical analysis on the data and draw conclusions such as: ‘on average students rated their professors 4.4’.

Qualitative research approach

You conduct in-depth interviews with 15 students and ask them open-ended questions such as: ‘How satisfied are you with your studies?’, ‘What is the most positive aspect of your study program?’ and ‘What can be done to improve the study program?’

Based on the answers you get you can ask follow-up questions to clarify things. You transcribe all interviews using transcription software and try to find commonalities and patterns.

Mixed methods approach

You conduct interviews to find out how satisfied students are with their studies. Through open-ended questions you learn things you never thought about before and gain new insights. Later, you use a survey to test these insights on a larger scale.

It’s also possible to start with a survey to find out the overall trends, followed by interviews to better understand the reasons behind the trends.

Qualitative or quantitative data by itself can’t prove or demonstrate anything, but has to be analysed to show its meaning in relation to the research questions. The method of analysis differs for each type of data.

Analysing quantitative data

Quantitative data is based on numbers. Simple maths or more advanced statistical analysis is used to discover commonalities or patterns in the data. The results are often reported in graphs and tables.

Applications such as Excel, SPSS, or R can be used to calculate things like:

• Average scores
• The number of times a particular answer was given
• The correlation or causation between two or more variables
• The reliability and validity of the results

Analysing qualitative data

Qualitative data is more difficult to analyse than quantitative data. It consists of text, images or videos instead of numbers.

Some common approaches to analysing qualitative data include:

• Qualitative content analysis : Tracking the occurrence, position and meaning of words or phrases
• Thematic analysis : Closely examining the data to identify the main themes and patterns
• Discourse analysis : Studying how communication works in social contexts

Quantitative research deals with numbers and statistics, while qualitative research deals with words and meanings.

Quantitative methods allow you to test a hypothesis by systematically collecting and analysing data, while qualitative methods allow you to explore ideas and experiences in depth.

In mixed methods research , you use both qualitative and quantitative data collection and analysis methods to answer your research question .

The research methods you use depend on the type of data you need to answer your research question .

• If you want to measure something or test a hypothesis , use quantitative methods . If you want to explore ideas, thoughts, and meanings, use qualitative methods .
• If you want to analyse a large amount of readily available data, use secondary data. If you want data specific to your purposes with control over how they are generated, collect primary data.
• If you want to establish cause-and-effect relationships between variables , use experimental methods. If you want to understand the characteristics of a research subject, use descriptive methods.

Data collection is the systematic process by which observations or measurements are gathered in research. It is used in many different contexts by academics, governments, businesses, and other organisations.

There are various approaches to qualitative data analysis , but they all share five steps in common:

• Prepare and organise your data.
• Review and explore your data.
• Develop a data coding system.
• Assign codes to the data.
• Identify recurring themes.

The specifics of each step depend on the focus of the analysis. Some common approaches include textual analysis , thematic analysis , and discourse analysis .

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Raimo Streefkerk

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• Published: 27 May 2024

Hypothalamic protein profiling from mice subjected to social defeat stress

• Shiladitya Mitra   ORCID: orcid.org/0000-0002-0741-9424 1 , 2   na1 ,
• Ghantasala S. Sameer Kumar 2   na1 ,
• Anumita Samanta 2 , 3 ,
• Mathias V. Schmidt 1 &
• Suman S. Thakur 2  

Molecular Brain volume  17 , Article number:  30 ( 2024 ) Cite this article

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The Hypothalmic-Pituitary-Adrenal axis also known as the HPA axis is central to stress response. It also acts as the relay center between the body and the brain. We analysed hypothalamic proteome from mice subjected to chronic social defeat paradigm using iTRAQ based quantitative proteomics to identify changes associated with stress response. We identified greater than 2000 proteins after processing our samples analysed through Q-Exactive (Thermo) and Orbitrap Velos (Thermo) at 5% FDR. Analysis of data procured from the runs showed that the proteins whose levels were affected belonged primarily to mitochondrial and metabolic processes, translation, complement pathway among others. We also found increased levels of fibrinogen, myelin basic protein (MBP) and neurofilaments (NEFL, NEFM, NEFH) in the hypothalamus from socially defeated mice. Interestingly, research indicates that these proteins are upregulated in blood and CSF of subjects exposed to trauma and stress. Since hypothalamus secreted proteins can be found in blood and CSF, their utility as biomarkers in depression holds an impressive probability and should be validated in clinical samples.

Introduction

Depression is one of the major causes of morbidity worldwide. Depression can arise due to genetic as well as environmental factors. Despite a high rate of prevalence of depression, its causes and proper treatment still remains elusive [ 1 ]. In this context, animal models of depression are of interest as they aid in understanding the etio-pathology of depression, finding molecular causes and well as diagnostic methods and screening of drugs. Social defeat model has been designed in a manner that it mimics multiple human situations like bullying, getting beaten up, physical abuse etc. [ 2 ]. This make this model an interesting choice to be studied and analysed, results of which may be correlated to human beings.

Multiple brain regions have been studied that may have a profound influence on the pathology of depression. This includes the hippocampus, amygdala, nucleus accumbens, pre-frontal cortex etc. [ 3 ]. A study investigating the proteomics changes in the hippocampus associated with susceptibility and resilience in chronic social defeat stress model identified LRP6, NPY and NPY2R as key molecules [ 4 ]. In another study, Lipocalin 2 (LCN2) identified again from hippocampus was attributed as a key player in susceptibility in a repeated social defeat stress model [ 5 ]. A proteomic study using repeated psycho-social stress model in rats, had identified proteins in the hippocampus belonging to energy metabolism, cytoskeleton, synaptic functions and chaperone activity to be differentially expressed in the stressed group as compared to controls [ 6 ]. Interestingly using CSDS model, Guo et al. had shown that ephrin receptor signaling pathway, particularly EPBH6 and Erk pathway to be differentially regulated in the pre-frontal cortex proteome [ 7 ]. Thus, while certain molecular changes might be global, different regions of the brain exhibit specific changes in response to stress – particularly social defeat stress.

The Hypothalamic Pituitary Adrenal axis (HPA axis) is at the foremost in response of the animal to stress as it acts as a relay center for the brain and the body. Theoretically, modulation of the HPA axis can make an animal resilient or more susceptible to stress [ 8 ]. The expression and release of gluco-corticoids—which are key to stress response, are regulated by the HPA axis [ 9 , 10 ]. The hypothalamus is the main orchestrator of the HPA axis. While the action of HPA axis when an animal is subjected to stress, is known, interestingly molecular changes in the hypothalamus that might be instrumental in understanding stress response—notably in case when an animal is subjected to social defeat stress, is understudied. The hypothalamus, particularly ventromedial hypothalamus has been shown to be critical to be part of neural circuitry for coping with social defeat stress [ 11 ]. Studies have been performed on hypothalamus for investigating differentially expressed genes after social defeat stress [ 12 , 13 ]. But a proteomics analysis has not been performed and only correlation of genes to proteins had been mentioned in literature. That said, hypothalamus from rats have been subjected to proteomics analysis after Chronic Mild Stress where a subset of genes has been identified and attributed to susceptibility [ 14 ]. Similar stress model in mice led to identification of proteins involved in glutamate balance and energy metabolism to be dysregulated in hypothalamic proteome [ 15 ]. In a lipo-polysaccharide induced depression model, proteins belonging to Ephrin receptor pathway, Glutamate transmission and AKT pathway was found to be altered in the hypothalamus of stressed mice [ 16 ]. But it must be noted that changes in protein levels can differ depending upon the stressor and the intensity of it. It has also been studied in sleep wake cycle [ 17 ] and obesity [ 18 ] among other conditions. We hope that this study adds further information and aids in understanding of hypothalamic changes under different stressors.

As stated earlier, molecular changes that may happen to the hypothalamus when an animal is subjected to chronic stress may reveal the mode of action of the hypothalamic regions and may also indicate potential bio-markers. Mass spectrometry, particularly iTRAQ based quantitative proteomics has developed into a powerful tool to identify potential bio-marker(s) [ 19 , 20 , 21 ]. Hence by employing proteomics to analyse hypothalamus of a mice subjected to social defeat stress, we attempted to find molecular changes that may be extrapolated to depressed human subjects.

Materials and methods

Animals used.

We used male C57Bl/6 J mice at 3–4 months of age for our experiment. The mice were housed in our in-house facility with 12 h of light and dark cycle. Food was provided ad-libitum. CD1 mice were chosen as the aggressor strain. For this purpose CD1 mice, which were retired breeders, were used. All the experiments were approved by Institutional Animal Ethics Committee (IAEC, CCMB; Reg. No. CPCSEA 20/1999).

Social defeat experiment

We followed a modified protocol that has been previously described [ 22 , 23 ]. Briefly, CD1 and C57Bl/6 J mice were housed together with a transparent partition between them. Once a day for 10 min over 10 days, C57Bl/6 J mice were placed in the partition of the CD1 mice ( n  = 10) and were allowed to bully the former. Care was taken that no physical injury was inflicted on the C57Bl/6 J strain. A Latin square approach was followed to prevent habituation.

A separate set of C57Bl/6 J mice ( n  = 7) were not exposed to social defeat served as wild-type controls.

Behavioral experiment

We performed social avoidance experiment as a test for defeat. The protocol followed has been discussed in other reports [ 22 , 23 ]. In short, C57Bl/6 J mice were kept in an open field with a transparent grilled enclosure/cage at one side. It was either empty or contained a CD1 aggressor mice. Area around the box was demarcated as interaction zone. Using Noldus Ethovision we tracked the duration and the path of the C57Bl/6 J mice in the interaction zone in the presence or absence of the aggressor. A ratio between the two was calculated as the interaction ratio and was measured as a percentage of total time spent in both conditions.

Sample collection

Mice were sacrificed the day after the behavioral tests were performed within 3 h of one round of social defeat and hypothalami were collected and flash frozen in liquid nitrogen for further analysis. Social avoidance data showed that interaction ratio of the defeated group to be significantly lower than that of the controls ( p  < 0.01). We selected 7 mice based on interaction ratio from the cohort and randomly assigned 3 mice for proteomics experiment and 4 mice for validation using Western Blotting and real time rtPCR analysis.

Proteomic experiment

Samples were processed and analysis was carried out using methods described previously [ 24 ]. Data are available via ProteomeXchange with identifier PXD005644. Briefly,

iTRAQ 4 plex: Three each of wild-type and social defeat mice were selected and hypothalamus were collected and pooled. We used 0.5% SDS to extract the proteins. After quantification using BCA kit (Thermo Fischer), we used 200ug of protein from both groups to proceed with labelling reaction. The protocol followed was as described by the manufacturer (ABSciex). TCEP (tris (2-carboxyethyl) phosphine) was used to reduce and methyl methanethiosulfonate (MMTS)- a cysteine blocker for reducing the samples. Samples were subsequently digested with trypsin (Promega Cat#:V511A) overnight using 1:20 (w/w) at 37 °C. 100 ug of each sample was used for technical duplicate and labelled with iTRAQ 4plex (ABSciex) reagents as per manufacturer’s protocol. Peptides from wild type samples were labelled with 114 and 115 tags, while those from social defeat samples were labelled with 116 and 117 tags. After labelling, samples were pooled, desalted using C18 spin columns (Pierce®) as per manufacturer’s protocol. The samples were subsequently processed for LC–MS/MS analysis after SCX fractionation using protocol described in a previous study [ 25 ].

LC–MS/MS analysis: Samples were analyzed on UPLC (Dionex The UltiMate® 3000 HPLC) interfaced Orbitrap Analyser (Thermo Scientific, Bremen, Germany) or Q-Exactive. Labelled peptides were introduced in a 15 cm long column (EASY-Spray column ES800, 15 cm × 75 µm ID, PepMap C18, 3 µm) and heated to 30° C. Peptides were separated using linear gradient from 2 to 98% of buffer B (95% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min. The gradient length was 50 min. Normalized collision energy (NCE) was set to 27 for fragmentation. Precursor ions were selected based on the charge state with priority of 2 + , 3 + and > 3 + . The dynamic exclusion was set as 30 s during data acquisition. The nano source was operated with 2.2 kV and the capillary temperature at 300 °C.

Data analysis: The data was analysed using Proteome Discoverer (Thermo Scientific, Bremen, Germany) software. NCBI fasta (for mouse) database was used to search for peptides. The workflow consisted of spectrum files, spectrum selector, reporter ion quantification and Sequest. Parameters included Methylthio (C), iTRAQ label at N-terminus of peptide. Lysine were used as a fixed modifications, whereas oxidation of methionine (M) and deamination (N/Q) were used as variable modification. The parent and fragment mass error tolerance were set as 20 ppm and 0.2 Da respectively. Using data from runs from both the machines, we acquired a total 77,751 MS/MS spectra and a total of 23,100 sequences were searched. We applied 5% FDR in our analysis and proteins with minimum of 1 unique peptide were considered.

Real time analysis

cDNA was prepared from the hypothalamus of wild-type and social defeated C57Bl/6 J mice ( n  = 4) using protocol listed elsewhere [ 26 ]. Shortly, RNA was extracted using Trizol (Sigma) and 1-Bromo 3-Chloror Propane followed by isopropanol precipitation of aqueous layer. cDNA was prepared using random primers and manufacturer’s instruction (Primescript cDNA synthesis kit, Takara). Real time analysis was performed using Sybr Green (Invitrogen) reagent in ABI HT 4900 machine according the manufacturer’s instructions and with 58℃ as the annealing temperature. The primers used for the experiment are listed below:

Western blotting

Proteins from hypothalamus from wild type ( n  = 4) and social defeat ( n  = 4) were extracted in RIPA buffer supplemented with Protease Cocktail Inhibitor. 50ug was loaded in an 10% SDS PAGE gel and transferred onto Nitrocellulose membrane using iBlot dry transfer (Invitrogen) according to manufacturer’s recommendations and blotted with antibodies against Neurofilament Medium (NF-M) (Proteintech 20664-1-AP, 1:1000) and Vinculin as housekeeping (Cell Signaling E1E9V, 1:1000). Images were taken with Chemidoc (BioRad).

Statistical analysis

Behavioral data were subjected to students t-test. Data from real time PCR and Western Blotting were subjected to non-parametric test. Proteomics data was subjected to FDR analysis.

The HPA axis is one of the major circuits that play an important role in an organism’s stress response. The hypothalamus is central to the HPA axis’ response to stress [ 27 ]. We carried out 4plex iTRAQ quantitative proteomic experiment to study the molecular changes in the hypothalamus when mice were subjected to chronic stress specifically social defeat stress (Fig.  1 A).

A Experimental outline. Social Defeat Stress procedure —Male CD1 and C57Bl/6 J are housed in one cage with transparent perforated partition in between. CD1 mice allowed to interact (bully) with the C57Bl/6 J mice for 10 min every day for 10 days. Analysis of chronic stress by Social Avoidance Test - C57BL/6 J mice of defeated and control groups are allowed to explore a box containing a target (CD1 mice) in one small perforated grilled cage. The social interaction ratio is calculated as the ratio between time spent in the interaction zone with the target compared to without the presence of the target. Sample processing and analysis – Mice were sacrificed and hypothalami were collected and subjected to either iTRAQ based proteomics or validation through rtPCR and Western Blotting. B The time spent by the defeated mice (SD) in the interaction zone was significantly lesser ( p  < 0.05) in the presence of a target as compared to without the target. There was significant difference in the time spent in the interaction zone by the unstressed controls (Wt). C The social interaction ratio was significantly lesser ( p  < 0.01) in the defeated group (SD) than the unstressed controls (Wt). Graphical figures have been generated by BioRender

A total of 17 mice were used for this study. While 7 mice were used as unstressed controls, 10 mice were subjected to social defeat paradigm. Mice subjected to social defeat stress spent significantly less time ( p  < 0.05) in the interaction zone with the presence of the target than without (Fig.  1 B). The non-stressed controls did not show any significant differences. Equivalently, the group of mice subjected to social defeat stress showed significantly lesser interaction ratio ( p  < 0.01) as compared to the controls (Fig.  1 C).

We used iTRAQ based quantitative proteomics to search for differentially expressed proteins in the hypothalamus of mice after chronic stress. In our analysis, combining runs from both Orbitrap Velos and Q-Exactive, we were able to identify more than 2000 unique proteins (Supplementary Table 1). We found a total of 181 proteins upregulated (> 1.5 folds (116 + 117/113 + 114 or social defeat/unstressed) in the hypothalamus of the mice subjected to social defeat stress as compared to the unstressed controls (Supplementary Table 2). In our analysis we failed to find any major downregulated proteins. The most plausible reason behind this would be technical limitations of our runs. However, it has been reported that after social defeat, the number of genes that were downregulated in the hypothalamus were considerably lesser than those upregulated [ 13 ].

The upregulated proteins were subjected to network analysis using STRING software v 12.0. They were arranged based on KEGG and GO Biological Processes pathway analysis using the same program. The top 6 KEGG pathways and top 10 Biological Processes affected have been depicted in Fig.  2 B and in Supplementary Table 3 and 4 respectively. Network analysis was carried out with medium confidence setting with only primary nodes. The proteins were clustered using k-means clustering in 10 prominent interacting groups (Fig.  2 , Supplementary Table 5). The groups consisted of the proteins (based on Biological Processes) associated with response to stress and biological regulation (cluster 1), structure and development (cluster 2), nervous system development and neurofilament (cluster 3), translation and metabolic processes (cluster 4), mitochondrial electron transport chain and cellular respiration (cluster 5), redox, ROS and homeostasis pathways (cluster 6), microtubule and cell adhesion (cluster 7), chromatin and nucleosome (cluster 8), spliceosome (cluster 9) and transcriptional regulation (cluster 10).

A Medium confidence network map of upregulated proteins (SD/Wt)—with thickness of lines depicting increasing confidence (0.15–0.90). k- means clustering with 10 clusters (dotted lines) was applied on these proteins. The network maps have been generated using String v.12. Each color represents a cluster. B Top 6 KEGG pathways and top 10 Biological processes affected from analysed proteins. SD: Social Defeat group, Wt: control group

Since stress might be affecting gene expression at the transcription level, we wanted to check whether upregulation was there at the transcript level of the genes whose protein levels were found upregulated in our study. We selected 4 proteins to check for their respective mRNA levels.

Gata1 is a transcription factor that has been shown to be associated with depression [ 28 ]. Though it was not picked up in our proteomics analysis, we decided to do a qPCR analysis of the same since being a master regulator its change would indicate validation of the model.

Hspe1 is subunit of heat shock protein. Heat shock proteins are transcription factors that have been found to be involved in multiple disorders and have been found to be upregulated under stressful conditions [ 29 ].

Nefm is one of the three neurofilaments that we indicated in our manuscript can be used as biomarkers for social defeat or trauma induced depression in concurrence with existing literature [ 30 ]. We wanted to check if the upregulation of Nefm is only at protein level or at mRNA level as well – the latter would indicate that it is transcriptionally upregulated as well.

Vimentin is an intermediate filament protein that has been shown to play a critical role in stress response [ 31 ]. It also interacts with multiple proteins including 14–3-3 which has been shown to be involved in stress adaptation [ 32 ]

Interestingly, we found equivalent changes in the transcript levels of Gata1, Hspe1 , Nefm and Vim ( p  < 0.05; Fig.  3 ) thus indicating that the changes at protein levels a result of direct transcriptional regulation. Its noteworthy that protein level changes may not correlate to mRNA level changes, however the reverse has more chances to be true and aids to confidence in the data set generated [ 33 ].

A qPCR analysis of genes was carried out for proteins found upregulated. Equivalently transcripts of Gata1, Hspe1, Nefm and Vim were found to be upregulated ( p  < 0.05) in defeated group (SD) as compared to unstressed controls (Wt); indicating that the effect of social defeat stress might be at the transcriptional level. B Western Blotting analysis of levels of NEFM to housekeeping Vinculin and relative density plot showing increased protein levels of NEFM in Social Defeat (SD) group as compared to controls (Wt). * Indicates p  < 0.05

To further validate our findings we decided to perform a Western Blotting analysis of one of the key proteins from our data—Neurofilament Medium (NEFM). The result (Fig.  3 B) concurred with our proteomics and qPCR data.

Discussions

Social Defeat induces molecular as well as physiological changes in the brain. Multiple studies have been done to see the effect of chronic stress like social defeat in different brain regions like hippocampus, amygdala, pre-frontal cortex etc. [ 34 , 35 , 36 , 37 ]. In this present study, we analysed protein profile of hypothalamus, the major constituent of the HPA axis to understand the molecular changes that take place in hypothalamus on subjection to social defeat stress and whether we can correlate them to the response to stress.

A considerable fraction of the proteins found up regulated in the hypothalamus of stressed mice as compared to the unstressed controls belonged to the mitochondrial oxidation–reduction pathway and metabolism. Oxidative stress has been shown to be one of the major players behind pathogenesis of multiple brain disorders like Alzheimers, Parkinson, Huntington and Depression [ 38 , 39 ]. We found peroredoxin and thioredoxin, both potent anti-oxidants to be upregulated. We also found upregulation in Cytochrome c, which also has anti-oxidant properties. Cytochrome c can bind to super-oxides produced from electron transport chain in mitochondria and neutralise the free radical. Thus, Cytochrome c aids in limiting and regulating the production of oxygen free radicals which may lead to oxidative stress [ 40 ]. Similarly, there was an upregulation in the levels of glutathione S-transferases (GST). GST has been known for its role in inactivating electrophilic compounds and for de-toxification [ 41 , 42 , 43 , 44 ]. Hence upregulation of these anti-oxidants might be a response to the build up of free radicals due to oxidative stress generated due to the chronic stress of social defeat in hypothalamus of mice.

We found upregulation in levels of neurofilaments NEFL, NEFM and NEFH. Transcript analysis indicates that NEFM is also transcriptionally upregulated. Neurofilaments are part of the axo-skeleton and also participate in axonal transport. NF may be accumulated in injury due to mechanical failure of the NF network [ 45 ]. In multiple neurodegenerative conditions, NFs acts as a plasma [ 46 ] and blood [ 47 ] biomarkers for axonal injury and neuronal damage. Interestingly, NEFL has been found to be significantly upregulated in CSF of a concussed boxer even 30 days post-concussion indicating its usefulness as a biological marker for injury and recovery [ 48 ]. Taken together, the detection of elevated neurofilament in hypothalamus of mice brain post social defeat stress indicates two points. Firstly, it validates social defeat stress as a model for depression. Secondly, as in the case of concussed boxers, the present study uphold the potential of neurofilaments to be used as biomarkers in several human traumatic/stressful events akin to social defeat model in mice viz., bullying, aggression, subordination and humiliation. Recently few studies have shown that this might be just the case [ 49 , 50 , 51 ].

In this study, we observed upregulation of Myelin Basic Protein (MBP) and associated proteins in the brain following stress in accordance with previous studies. MBP has been associated with neuronal death and trauma and has been found to be increased in CSF of dogs post traumatic brain injury [ 52 , 53 ].

Additionally, upregulation in the levels of several RPS subunits, like RPS14, 26 (a ribosomal protein subunit) were also observed. Ribosomal genes have been found to be upregulated in one more study that analysed de-regulated gene expression in the hypothalamus post social defeat stress [ 13 ]. This validates our finding at transcriptional level and also reflects upon the importance of protein translation under stress.

Changes were also found in levels of S100beta, a marker of astrocytes. An increase may indicate towards increased reactive astrocytes as a result of stress [ 54 , 55 ]. Similar lines hold for microglia, which has already been shown to be increased in social defeat model and in other stress models [ 56 ]. An increase in CDC42 found in our proteomic dataset indicated to possible increase in microglial activation as a result of social defeat [ 57 , 58 ].

We also found increased expression of Fibrinogen alpha, beta and gamma chains along with elevated levels of ApoA1. A complex of alpha–beta Fibrinogen can activate microglia thus leading to neuro-degeneration [ 59 ]. Fibrinogen and ApoA1 usually accumulate in the brain from the periphery [ 59 , 60 ] and their increased levels in hypothalamus might indicate compromised Blood Brain Barrier (BBB) as a result of social defeat stress. In fact recent investigations have shown that leaky BBB might be associated with different regions of brain in mice subjected to stressful paradigms including social defeat and associated with progression of depression like phenotype [ 61 , 62 , 63 ]. This might also facilitate the movement of NEFM, NEFL and S100beta into the CSF – the elevated levels of which have been reported in clinical samples from individuals subjected to stress, particularly Traumatic Stress. This re-ignites the debate whether social defeat model is a model for depression like symptoms or ideally a model of PTSD (post-traumatic stress disorder) [ 64 ].

Of the different proteins found up regulated, SLC4A1 also deserves special attention as it has been shown to be a promising biomarker in patients with MDD [ 65 ].

Several researches have been performed for transcriptomic analysis of different brain regions after social defeat stress. Few of the genes from meta-analysis performed on those studies have been also found in our data set. These include ribosomal genes, heamoglobin genes (Hbb-bs), myelination genes (Mbp), stress and response to insult (Col1a1, Slc25a5) [ 66 , 67 ]. However, it must be noted that majority of the studies have been performed from hippocampus, prefrontal cortex, amygdala, nucleus accumbens and Ventral Tegmental area, with very few from Hypothalamus and bed nucleus of stria terminalis.

It must be noted that in our study after social defeat stress, we did not segregate the groups into susceptible and resilient populations. The analysis of the proteins can thus be done as a future follow up study after such classification. This can yield further information on protein levels and behavioral outcomes. However, based on our qPCR and western blot analysis, we are confident that at least a subset of the proteins, particularly the neurofilaments show an uniform increase in their levels in socially defeated groups as compare to the controls, thus increasing our confidence in them as potential biomarkers. Interestingly, a subset of the proteins identified in this study including the neurofilament proteins have also been identified to be dysregulated in hypothalamic proteome of rats after chronic mild stress [ 14 ].

Taken together, this present study gives information about de-regulated proteins in the hypothalamus of social defeat stressed mice. A large number of changes have been found in multiple studies in different brain regions. We found a considerable proportion of proteins in our data to be similar to those reported in the literature. Changes in hypothalamus are interesting bearing in mind that it interacts directly with the peripheral circulation with modified BBB. We report markers for neuronal death and atrophy and add weightage to the notion that neurofilaments like NEFL, NEFM and MBP may act as potential biomarkers in human stress and traumatic disorders.

Availability of data and materials

The mass spectrometry proteomics (raw) data have been deposited to and available at the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD005644. The final processed data has been included in the Supplementary Information.

Abbreviations

Hypothalamic-Pituitary-Adrenal axis

Neurofilament

Ribosomal Protein Subunit

Blood Brain Barrier

Major Depressive Disorder

Cerebrospinal Fluid

Hollis F, Kabbaj M. Social defeat as an animal model for depression. ILAR J. 2014;55(2):221–32.

Article   CAS   PubMed   Google Scholar  

Krishnan V, Nestler EJ. Animal models of depression: molecular perspectives. Curr Top Behav Neurosci. 2011;7(1):121–47.

Article   PubMed   PubMed Central   Google Scholar  

Nestler EJ, Barrot M, DiLeone RJ, Eisch AJ, Gold SJ, Monteggia LM. Neurobiology of depression. Neuron. 2002;34:13–25.

He Y, Li W, Tian Y, Chen X, Cheng K, Xu K, et al. iTRAQ-based proteomics suggests LRP6, NPY and NPY2R perturbation in the hippocampus involved in CSDS may induce resilience and susceptibility. Life Sci. 2018;211:102–17.

Chen YH, Xie SY, Chen CW, Lu DY. Electroacupuncture improves repeated social defeat stress-elicited social avoidance and anxiety-like behaviors by reducing Lipocalin-2 in the hippocampus. Mol Brain. 2021;14(1):150.

Article   CAS   PubMed   PubMed Central   Google Scholar  

Carboni L, Piubelli C, Pozzato C, Astner H, Arban R, Righetti PG, et al. Proteomic analysis of rat hippocampus after repeated psychosocial stress. Neuroscience. 2006;137(4):1237–46.

Guo H, Huang ZL, Wang W, Zhang SX, Li J, Cheng K, et al. iTRAQ-based proteomics suggests Ephb6 as a potential regulator of the ERK pathway in the prefrontal cortex of chronic social defeat stress model mice. Prot Clin Appl. 2017;11:1700115.  https://doi.org/10.1002/prca.201700115 .

Pfau ML, Russo SJ. Peripheral and central mechanisms of stress resilience. Neurobiol Stress. 2015;1:66–79.

Article   PubMed   Google Scholar  

Sapolsky RM, Romero LM, Munck AU. How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocr Rev. 2000;21:55–89.

CAS   PubMed   Google Scholar  

Reul JMHM, Collins A, Saliba RS, Mifsud KR, Carter SD, Gutierrez-Mecinas M, et al. Glucocorticoids, epigenetic control and stress resilience. Neurobiol Stress. 2015;1:44–59.

Diaz V, Lin D. Neural circuits for coping with social defeat. Curr Opin Neurobiol. 2020;60:99–107. Available from: https://www.sciencedirect.com/science/article/pii/S0959438819301278 .

Galyamina AG, Smagin DA, Kovalenko IL, Redina OE, Babenko VN, Kudryavtseva NN. The dysfunction of carcinogenesis- and apoptosis-associated genes that develops in the hypothalamus under chronic social defeat stress in male mice. Biochem Mosc. 2022;87(9):1050–64. https://doi.org/10.1134/S0006297922090152 .

Article   CAS   Google Scholar  

Smagin DA, Kovalenko IL, Galyamina AG, Bragin AO, Orlov YL, Kudryavtseva NN. Dysfunction in ribosomal gene expression in the hypothalamus and hippocampus following chronic social defeat stress in male mice as revealed by RNA-Seq. Neural Plast. 2016;2016:3289187.

Gong W, Liao W, Fang C, Liu Y, Xie H, Yi F, et al. Analysis of chronic mild stress-induced hypothalamic proteome: identification of protein dysregulations associated with vulnerability and resiliency to depression or anxiety. Front Mol Neurosci. 2021;14:633398. Available from: https://www.frontiersin.org/articles/10.3389/fnmol.2021.633398 .

Rao C, Shi H, Zhou C, Zhu D, Zhao M, Wang Z, et al. Hypothalamic proteomic analysis reveals dysregulation of glutamate balance and energy metabolism in a mouse model of chronic mild stress-induced depression. Neurochem Res. 2016;41(9):2443–56.

Wu Y, Wei Z, Li Y, Wei C, Li Y, Cheng P, et al. Perturbation of ephrin receptor signaling and glutamatergic transmission in the hypothalamus in depression using proteomics integrated with metabolomics. Front Neurosci. 2019;13:492187.

Article   Google Scholar  

Jha PK, Valekunja UK, Ray S, Nollet M, Reddy AB. Single-cell transcriptomics and cell-specific proteomics reveals molecular signatures of sleep. Commun Biol. 2022;5(1):846. https://doi.org/10.1038/s42003-022-03800-3 .

Manousopoulou A, Koutmani Y, Karaliota S, Woelk CH, Manolakos ES, Karalis K, et al. Hypothalamus proteomics from mouse models with obesity and anorexia reveals therapeutic targets of appetite regulation. Nutr Diabetes. 2016;6(4):e204. https://doi.org/10.1038/nutd.2016.10 .

DeSouza LV, Voisin SN, Siu KWM. ITRAQ-labeling for biomarker discovery. Methods Mol Biol. 2013;1002:105–14.

Crutchfield CA, Thomas SN, Sokoll LJ, Chan DW. Advances in mass spectrometry-based clinical biomarker discovery. Clin Proteomics. 2016;13:1.

Street JM, Dear JW. The application of mass-spectrometry-based protein biomarker discovery to theragnostics. Br J Clin Pharmacol. 2010;69:367–78.

Golden SA, Covington HE 3rd, Berton O, Russo SJ. A standardized protocol for repeated social defeat stress in mice. Nat Protoc. 2011;6(8):1183–91.

Wilkinson MB, Xiao G, Kumar A, LaPlant Q, Renthal W, Sikder D, et al. Imipramine treatment and resiliency exhibit similar chromatin regulation in the mouse nucleus accumbens in depression models. J Neurosci. 2009;29(24):7820–32.

Mitra S, Sameer Kumar GS, Jyothilakshmi L, Thakur SS, Kumar S. Absence of Wdr13 gene predisposes mice to mild social isolation - chronic stress, leading to depression-like phenotype associated with differential expression of synaptic proteins. Front Mol Neurosci. 2018;11:133.

Venugopal AK, Ghantasala SSK, Selvan LDN, Mahadevan A, Renuse S, Kumar P, et al. Quantitative proteomics for identifying biomarkers for Rabies. Clin Proteomics. 2013;10(1):3.

Mitra S, Sameer Kumar GS, Tiwari V, Lakshmi BJ, Thakur SS, Kumar S. Implication of genetic deletion of Wdr13 in mice: mild anxiety, better performance in spatial memory task, with upregulation of multiple synaptic proteins. Front Mol Neurosci. 2016;9:73.

Smith SM, Vale WW. The role of the hypothalamic-pituitary-adrenal axis in neuroendocrine responses to stress. Dialogues Clin Neurosci. 2006;8:383–95.

Kang HJ, Voleti B, Hajszan T, Rajkowska G, Stockmeier CA, Licznerski P, et al. Decreased expression of synapse-related genes and loss of synapses in major depressive disorder. Nat Med. 2012;18(9):1413–7.

Gomez-Pastor R, Burchfiel ET, Thiele DJ. Regulation of heat shock transcription factors and their roles in physiology and disease. Nat Rev Mol Cell Biol. 2018;19(1):4–19. https://doi.org/10.1038/nrm.2017.73 .

Khalil M, Teunissen CE, Otto M, Piehl F, Sormani MP, Gattringer T, et al. Neurofilaments as biomarkers in neurological disorders. Nat Rev Neurol. 2018;14(10):577–89. https://doi.org/10.1038/s41582-018-0058-z .

Pérez-Sala D, Oeste CL, Martínez AE, Carrasco MJ, Garzón B, Cañada FJ. Vimentin filament organization and stress sensing depend on its single cysteine residue and zinc binding. Nat Commun. 2015;6(1):7287. https://doi.org/10.1038/ncomms8287 .

Pennington KL, Chan TY, Torres MP, Andersen JL. The dynamic and stress-adaptive signaling hub of 14–3–3: emerging mechanisms of regulation and context-dependent protein–protein interactions. Oncogene. 2018;37(42):5587–604. https://doi.org/10.1038/s41388-018-0348-3 .

Buccitelli C, Selbach M. mRNAs, proteins and the emerging principles of gene expression control. Nat Rev Genet. 2020;21(10):630–44. https://doi.org/10.1038/s41576-020-0258-4 .

Fan L, Yang L, Li X, Teng T, Xiang Y, Liu X, et al. Proteomic and metabolomic characterization of amygdala in chronic social defeat stress rats. Behav Brain Res. 2021;412:113407.

Iñiguez SD, Riggs LM, Nieto SJ, Dayrit G, Zamora NN, Shawhan KL, et al. Social defeat stress induces a depression-like phenotype in adolescent male c57BL/6 mice. Stress. 2014;17(3):247–55.

Iñiguez SD, Aubry A, Riggs LM, Alipio JB, Zanca RM, Flores-Ramirez FJ, et al. Social defeat stress induces depression-like behavior and alters spine morphology in the hippocampus of adolescent male C57BL/6 mice. Neurobiol Stress. 2016;5:54–64.

Colyn L, Venzala E, Marco S, Perez-Otaño I, Tordera RM. Chronic social defeat stress induces sustained synaptic structural changes in the prefrontal cortex and amygdala. Behav Brain Res. 2019;373:112079.

Jiménez-Fernández S, Gurpegui M, Díaz-Atienza F, Pérez-Costillas L, Gerstenberg M, Correll CU. Oxidative stress and antioxidant parameters in patients with major depressive disorder compared to healthy controls before and after antidepressant treatment: results from a meta-analysis. J Clin Psychiatry. 2015;76(12):1658–67.

Liu T, Zhong S, Liao X, Chen J, He T, Lai S, et al. A meta-analysis of oxidative stress markers in depression. PLoS ONE. 2015;10(10):e0138904.

Bowman SEJ, Bren KL. The chemistry and biochemistry of heme c: functional bases for covalent attachment. Nat Prod Rep. 2008;25(6):1118–30.

Townsend DM, Tew KD, Tapiero H. The importance of glutathione in human disease. Biomed Pharmacother. 2003;57(3–4):145–55.

Townsend DM, Findlay VL, Tew KD. Glutathione S-transferases as regulators of kinase pathways and anticancer drug targets. Methods Enzymol. 2005;401:287–307.

Wu G, Fang YZ, Yang S, Lupton JR, Turner ND. Glutathione metabolism and its implications for health. J Nutr. 2004;134(3):489–92.

Tirona RG, Pang KS. Bimolecular glutathione conjugation kinetics of ethacrynic acid in rat liver: in vitro and perfusion studies. J Pharmacol Exp Ther. 1999;290(3):1230–41.

Meythaler JM, Peduzzi JD, Eleftheriou E, Novack TA. Current concepts: diffuse axonal injury-associated traumatic brain injury. Arch Phys Med Rehabil. 2001;82(10):1461–71.

Ashton NJ, Janelidze S, Al Khleifat A, Leuzy A, van der Ende EL, Karikari TK, et al. A multicentre validation study of the diagnostic value of plasma neurofilament light. Nat Commun. 2021;12(1):3400.

Anderson KJ, Scheff SW, Miller KM, Roberts KN, Gilmer LK, Yang C, et al. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury. J Neurotrauma. 2008;25(9):1079–85.

Neselius S, Zetterberg H, Blennow K, Marcusson J, Brisby H. Increased CSF levels of phosphorylated neurofilament heavy protein following bout in amateur boxers. PLoS ONE. 2013;8(11):e81249.

Chen MH, Liu YL, Kuo HW, Tsai SJ, Hsu JW, Huang KL, et al. Neurofilament light chain is a novel biomarker for major depression and related executive dysfunction. Int J Neuropsychopharmacol. 2022;25(2):99–105.

Bacioglu M, Maia LF, Preische O, Schelle J, Apel A, Kaeser SA, et al. Neurofilament light chain in blood and CSF as marker of disease progression in mouse models and in neurodegenerative diseases. Neuron. 2016;91(1):56–66.

Travica N, Berk M, Marx W. Neurofilament light protein as a biomarker in depression and cognitive function. Curr Opin Psychiatry. 2022;35(1):30–7.

Bohnert S, Wirth C, Schmitz W, Trella S, Monoranu CM, Ondruschka B, et al. Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury. Int J Legal Med. 2021;135(4):1525–35.

Su E, Bell MJ, Kochanek PM, Wisniewski SR, Bayir H, Clark RSB, et al. Increased CSF concentrations of myelin basic protein after TBI in infants and children: absence of significant effect of therapeutic hypothermia. Neurocrit Care. 2012;17(3):401–7.

Kleindienst A, Hesse F, Bullock MR, Buchfelder M. The neurotrophic protein S100B: value as a marker of brain damage and possible therapeutic implications. Prog Brain Res. 2007;161:317–25.

Borella A, Sumangali R, Ko J, Whitaker-Azmitia PM. Characterization of social behaviors and oxytocinergic neurons in the S-100 beta overexpressing mouse model of Down Syndrome. Behav Brain Res. 2003;141(2):229–36.

Stein DJ, Vasconcelos MF, Albrechet-Souza L, Ceresér KMM, de Almeida RMM. Microglial Over-activation by social defeat stress contributes to anxiety- and depressive-like behaviors. Front Behav Neurosci. 2017;11:207.

Barcia C, Ros CM, Annese V, Carrillo-de Sauvage MA, Ros-Bernal F, Gómez A, et al. ROCK/Cdc42-mediated microglial motility and gliapse formation lead to phagocytosis of degenerating dopaminergic neurons in vivo. Sci Rep. 2012;2(1):809.

de Pablos RM, Herrera AJ, Espinosa-Oliva AM, Sarmiento M, Muñoz MF, Machado A, et al. Chronic stress enhances microglia activation and exacerbates death of nigral dopaminergic neurons under conditions of inflammation. J Neuroinflammation. 2014;11:34.

Ryu JK, Davalos D, Akassoglou K. Fibrinogen signal transduction in the nervous system. J Thromb Haemost. 2009;7 Suppl 1(Suppl 1):151–4.

Zhou AL, Swaminathan SK, Curran GL, Poduslo JF, Lowe VJ, Li L, et al. Apolipoprotein A-I crosses the blood-brain barrier through clathrin-independent and cholesterol-mediated endocytosis. J Pharmacol Exp Ther. 2019;369(3):481–8.

Welcome MO, Mastorakis NE. Stress-induced blood brain barrier disruption: molecular mechanisms and signaling pathways. Pharmacol Res. 2020;157:104769.

Menard C, Pfau ML, Hodes GE, Kana V, Wang VX, Bouchard S, et al. Social stress induces neurovascular pathology promoting depression. Nat Neurosci. 2017;20(12):1752–60.

Dion-Albert L, Cadoret A, Doney E, Kaufmann FN, Dudek KA, Daigle B, et al. Vascular and blood-brain barrier-related changes underlie stress responses and resilience in female mice and depression in human tissue. Nat Commun. 2022;13(1):164.

Richter-Levin G, Stork O, Schmidt MV. Animal models of PTSD: a challenge to be met. Mol Psychiatry. 2019;24(8):1135–56.

Pajer K, Andrus BM, Gardner W, Lourie A, Strange B, Campo J, et al. Discovery of blood transcriptomic markers for depression in animal models and pilot validation in subjects with early-onset major depression. Transl Psychiatry. 2012;2(4):e101.

Flati T, Gioiosa S, Chillemi G, Mele A, Oliverio A, Mannironi C, et al. A gene expression atlas for different kinds of stress in the mouse brain. Sci Data. 2020;7(1):437. https://doi.org/10.1038/s41597-020-00772-z .

Reshetnikov VV, Kisaretova PE, Ershov NI, Merkulova TI, Bondar NP. Social defeat stress in adult mice causes alterations in gene expression, alternative splicing, and the epigenetic landscape of H3K4me3 in the prefrontal cortex: an impact of early-life stress. Prog Neuropsychopharmacol Biol Psychiatry. 2021;106:110068. Available from: https://www.sciencedirect.com/science/article/pii/S0278584620303845 .

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Acknowledgements

We thank the Brain and Behavior Facility at CCMB for their help and support with the experiment. We thank the Proteomic Facility at CCMB for aiding in running the samples and analysis. We thank Dr. Satish Kumar, CCMB for his support and feedback. We thank Dr. Margherita Springer, MPIP for her help and advice.

Open Access funding enabled and organized by Projekt DEAL. The work has been funded by CSIR-CCMB and Max Planck Institute for Psychiatry in house funding.

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Shiladitya Mitra and Ghantasala S. Sameer Kumar contributed equally to this work.

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Max Planck Institute of Psychiatry, Kraepelinstr 2-10, Munich, 80804, Germany

Shiladitya Mitra & Mathias V. Schmidt

CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Habsiguda, Hyderabad, 500007, India

Shiladitya Mitra, Ghantasala S. Sameer Kumar, Anumita Samanta & Suman S. Thakur

Donders Institute for Brain Cognition and Behavior, Radboud University, Postbs 9010, Nijmegen, 6500GL, Netherlands

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AS and SM performed behavioral analysis and collected samples. SM and GSS processed the samples. GSS performed mass spectrometry of the samples. SM and GSS analyzed the data. SM wrote the manuscript. ST gave intellectual and technical inputs and helped compiling the manuscript. MVS helped in performing experiments and gave valuable inputs.

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Mitra, S., Sameer Kumar, G.S., Samanta, A. et al. Hypothalamic protein profiling from mice subjected to social defeat stress. Mol Brain 17 , 30 (2024). https://doi.org/10.1186/s13041-024-01096-4

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Marketers often seek to understand their customers deeply. Qualitative research methods such as face-to-face interviews, focus groups, and qualitative observations can provide valuable insights into your products, your market, and your customers’ opinions and motivations. Understanding these nuances can significantly enhance marketing strategies and overall customer satisfaction.

What is Qualitative Research

Qualitative research is a market research method that focuses on obtaining data through open-ended and conversational communication. This method focuses on the “why” rather than the “what” people think about you. Thus, qualitative research seeks to uncover the underlying motivations, attitudes, and beliefs that drive people’s actions. 

Let’s say you have an online shop catering to a general audience. You do a demographic analysis and you find out that most of your customers are male. Naturally, you will want to find out why women are not buying from you. And that’s what qualitative research will help you find out.

In the case of your online shop, qualitative research would involve reaching out to female non-customers through methods such as in-depth interviews or focus groups. These interactions provide a platform for women to express their thoughts, feelings, and concerns regarding your products or brand. Through qualitative analysis, you can uncover valuable insights into factors such as product preferences, user experience, brand perception, and barriers to purchase.

Types of Qualitative Research Methods

Qualitative research methods are designed in a manner that helps reveal the behavior and perception of a target audience regarding a particular topic.

The most frequently used qualitative analysis methods are one-on-one interviews, focus groups, ethnographic research, case study research, record keeping, and qualitative observation.

1. One-on-one interviews

Conducting one-on-one interviews is one of the most common qualitative research methods. One of the advantages of this method is that it provides a great opportunity to gather precise data about what people think and their motivations.

Spending time talking to customers not only helps marketers understand who their clients are, but also helps with customer care: clients love hearing from brands. This strengthens the relationship between a brand and its clients and paves the way for customer testimonials.

• A company might conduct interviews to understand why a product failed to meet sales expectations.
• A researcher might use interviews to gather personal stories about experiences with healthcare.

These interviews can be performed face-to-face or on the phone and usually last between half an hour to over two hours. 

When a one-on-one interview is conducted face-to-face, it also gives the marketer the opportunity to read the body language of the respondent and match the responses.

2. Focus groups

Focus groups gather a small number of people to discuss and provide feedback on a particular subject. The ideal size of a focus group is usually between five and eight participants. The size of focus groups should reflect the participants’ familiarity with the topic. For less important topics or when participants have little experience, a group of 10 can be effective. For more critical topics or when participants are more knowledgeable, a smaller group of five to six is preferable for deeper discussions.

The main goal of a focus group is to find answers to the “why”, “what”, and “how” questions. This method is highly effective in exploring people’s feelings and ideas in a social setting, where group dynamics can bring out insights that might not emerge in one-on-one situations.

• A focus group could be used to test reactions to a new product concept.
• Marketers might use focus groups to see how different demographic groups react to an advertising campaign.

One advantage that focus groups have is that the marketer doesn’t necessarily have to interact with the group in person. Nowadays focus groups can be sent as online qualitative surveys on various devices.

Focus groups are an expensive option compared to the other qualitative research methods, which is why they are typically used to explain complex processes.

3. Ethnographic research

Ethnographic research is the most in-depth observational method that studies individuals in their naturally occurring environment.

This method aims at understanding the cultures, challenges, motivations, and settings that occur.

• A study of workplace culture within a tech startup.
• Observational research in a remote village to understand local traditions.

Ethnographic research requires the marketer to adapt to the target audiences’ environments (a different organization, a different city, or even a remote location), which is why geographical constraints can be an issue while collecting data.

This type of research can last from a few days to a few years. It’s challenging and time-consuming and solely depends on the expertise of the marketer to be able to analyze, observe, and infer the data.

4. Case study research

The case study method has grown into a valuable qualitative research method. This type of research method is usually used in education or social sciences. It involves a comprehensive examination of a single instance or event, providing detailed insights into complex issues in real-life contexts.  

• Analyzing a single school’s innovative teaching method.
• A detailed study of a patient’s medical treatment over several years.

Case study research may seem difficult to operate, but it’s actually one of the simplest ways of conducting research as it involves a deep dive and thorough understanding of the data collection methods and inferring the data.

5. Record keeping

Record keeping is similar to going to the library: you go over books or any other reference material to collect relevant data. This method uses already existing reliable documents and similar sources of information as a data source.

• Historical research using old newspapers and letters.
• A study on policy changes over the years by examining government records.

This method is useful for constructing a historical context around a research topic or verifying other findings with documented evidence.

6. Qualitative observation

Qualitative observation is a method that uses subjective methodologies to gather systematic information or data. This method deals with the five major sensory organs and their functioning, sight, smell, touch, taste, and hearing.

• Sight : Observing the way customers visually interact with product displays in a store to understand their browsing behaviors and preferences.
• Smell : Noting reactions of consumers to different scents in a fragrance shop to study the impact of olfactory elements on product preference.
• Touch : Watching how individuals interact with different materials in a clothing store to assess the importance of texture in fabric selection.
• Taste : Evaluating reactions of participants in a taste test to identify flavor profiles that appeal to different demographic groups.
• Hearing : Documenting responses to changes in background music within a retail environment to determine its effect on shopping behavior and mood.

Below we are also providing real-life examples of qualitative research that demonstrate practical applications across various contexts:

Qualitative Research Real World Examples

Let’s explore some examples of how qualitative research can be applied in different contexts.

1. Online grocery shop with a predominantly male audience

Method used: one-on-one interviews.

Let’s go back to one of the previous examples. You have an online grocery shop. By nature, it addresses a general audience, but after you do a demographic analysis you find out that most of your customers are male.

One good method to determine why women are not buying from you is to hold one-on-one interviews with potential customers in the category.

Interviewing a sample of potential female customers should reveal why they don’t find your store appealing. The reasons could range from not stocking enough products for women to perhaps the store’s emphasis on heavy-duty tools and automotive products, for example. These insights can guide adjustments in inventory and marketing strategies.

2. Software company launching a new product

Method used: focus groups.

Focus groups are great for establishing product-market fit.

Let’s assume you are a software company that wants to launch a new product and you hold a focus group with 12 people. Although getting their feedback regarding users’ experience with the product is a good thing, this sample is too small to define how the entire market will react to your product.

So what you can do instead is holding multiple focus groups in 20 different geographic regions. Each region should be hosting a group of 12 for each market segment; you can even segment your audience based on age. This would be a better way to establish credibility in the feedback you receive.

3. Alan Pushkin’s “God’s Choice: The Total World of a Fundamentalist Christian School”

Method used: ethnographic research.

Moving from a fictional example to a real-life one, let’s analyze Alan Peshkin’s 1986 book “God’s Choice: The Total World of a Fundamentalist Christian School”.

Peshkin studied the culture of Bethany Baptist Academy by interviewing the students, parents, teachers, and members of the community alike, and spending eighteen months observing them to provide a comprehensive and in-depth analysis of Christian schooling as an alternative to public education.

The study highlights the school’s unified purpose, rigorous academic environment, and strong community support while also pointing out its lack of cultural diversity and openness to differing viewpoints. These insights are crucial for understanding how such educational settings operate and what they offer to students.

Even after discovering all this, Peshkin still presented the school in a positive light and stated that public schools have much to learn from such schools.

Peshkin’s in-depth research represents a qualitative study that uses observations and unstructured interviews, without any assumptions or hypotheses. He utilizes descriptive or non-quantifiable data on Bethany Baptist Academy specifically, without attempting to generalize the findings to other Christian schools.

4. Understanding buyers’ trends

Method used: record keeping.

Another way marketers can use quality research is to understand buyers’ trends. To do this, marketers need to look at historical data for both their company and their industry and identify where buyers are purchasing items in higher volumes.

For example, electronics distributors know that the holiday season is a peak market for sales while life insurance agents find that spring and summer wedding months are good seasons for targeting new clients.

5. Determining products/services missing from the market

Conducting your own research isn’t always necessary. If there are significant breakthroughs in your industry, you can use industry data and adapt it to your marketing needs.

The influx of hacking and hijacking of cloud-based information has made Internet security a topic of many industry reports lately. A software company could use these reports to better understand the problems its clients are facing.

As a result, the company can provide solutions prospects already know they need.

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Qualitative Research Approaches

Once the marketer has decided that their research questions will provide data that is qualitative in nature, the next step is to choose the appropriate qualitative approach.

The approach chosen will take into account the purpose of the research, the role of the researcher, the data collected, the method of data analysis , and how the results will be presented. The most common approaches include:

• Narrative : This method focuses on individual life stories to understand personal experiences and journeys. It examines how people structure their stories and the themes within them to explore human existence. For example, a narrative study might look at cancer survivors to understand their resilience and coping strategies.
• Phenomenology : attempts to understand or explain life experiences or phenomena; It aims to reveal the depth of human consciousness and perception, such as by studying the daily lives of those with chronic illnesses.
• Grounded theory : investigates the process, action, or interaction with the goal of developing a theory “grounded” in observations and empirical data. 
• Ethnography : describes and interprets an ethnic, cultural, or social group;
• Case study : examines episodic events in a definable framework, develops in-depth analyses of single or multiple cases, and generally explains “how”. An example might be studying a community health program to evaluate its success and impact.

How to Analyze Qualitative Data

Analyzing qualitative data involves interpreting non-numerical data to uncover patterns, themes, and deeper insights. This process is typically more subjective and requires a systematic approach to ensure reliability and validity. 

1. Data Collection

Ensure that your data collection methods (e.g., interviews, focus groups, observations) are well-documented and comprehensive. This step is crucial because the quality and depth of the data collected will significantly influence the analysis.

2. Data Preparation

Once collected, the data needs to be organized. Transcribe audio and video recordings, and gather all notes and documents. Ensure that all data is anonymized to protect participant confidentiality where necessary.

3. Familiarization

Immerse yourself in the data by reading through the materials multiple times. This helps you get a general sense of the information and begin identifying patterns or recurring themes.

Develop a coding system to tag data with labels that summarize and account for each piece of information. Codes can be words, phrases, or acronyms that represent how these segments relate to your research questions.

• Descriptive Coding : Summarize the primary topic of the data.
• In Vivo Coding : Use language and terms used by the participants themselves.
• Process Coding : Use gerunds (“-ing” words) to label the processes at play.
• Emotion Coding : Identify and record the emotions conveyed or experienced.

5. Thematic Development

Group codes into themes that represent larger patterns in the data. These themes should relate directly to the research questions and form a coherent narrative about the findings.

6. Interpreting the Data

Interpret the data by constructing a logical narrative. This involves piecing together the themes to explain larger insights about the data. Link the results back to your research objectives and existing literature to bolster your interpretations.

7. Validation

Check the reliability and validity of your findings by reviewing if the interpretations are supported by the data. This may involve revisiting the data multiple times or discussing the findings with colleagues or participants for validation.

8. Reporting

Finally, present the findings in a clear and organized manner. Use direct quotes and detailed descriptions to illustrate the themes and insights. The report should communicate the narrative you’ve built from your data, clearly linking your findings to your research questions.

Limitations of qualitative research

The disadvantages of qualitative research are quite unique. The techniques of the data collector and their own unique observations can alter the information in subtle ways. That being said, these are the qualitative research’s limitations:

1. It’s a time-consuming process

The main drawback of qualitative study is that the process is time-consuming. Another problem is that the interpretations are limited. Personal experience and knowledge influence observations and conclusions.

Thus, qualitative research might take several weeks or months. Also, since this process delves into personal interaction for data collection, discussions often tend to deviate from the main issue to be studied.

2. You can’t verify the results of qualitative research

Because qualitative research is open-ended, participants have more control over the content of the data collected. So the marketer is not able to verify the results objectively against the scenarios stated by the respondents. For example, in a focus group discussing a new product, participants might express their feelings about the design and functionality. However, these opinions are influenced by individual tastes and experiences, making it difficult to ascertain a universally applicable conclusion from these discussions.

3. It’s a labor-intensive approach

Qualitative research requires a labor-intensive analysis process such as categorization, recording, etc. Similarly, qualitative research requires well-experienced marketers to obtain the needed data from a group of respondents.

4. It’s difficult to investigate causality

Qualitative research requires thoughtful planning to ensure the obtained results are accurate. There is no way to analyze qualitative data mathematically. This type of research is based more on opinion and judgment rather than results. Because all qualitative studies are unique they are difficult to replicate.

5. Qualitative research is not statistically representative

Because qualitative research is a perspective-based method of research, the responses given are not measured.

Comparisons can be made and this can lead toward duplication, but for the most part, quantitative data is required for circumstances that need statistical representation and that is not part of the qualitative research process.

While doing a qualitative study, it’s important to cross-reference the data obtained with the quantitative data. By continuously surveying prospects and customers marketers can build a stronger database of useful information.

Quantitative vs. Qualitative Research

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Quantitative and qualitative research are two distinct methodologies used in the field of market research, each offering unique insights and approaches to understanding consumer behavior and preferences.

As we already defined, qualitative analysis seeks to explore the deeper meanings, perceptions, and motivations behind human behavior through non-numerical data. On the other hand, quantitative research focuses on collecting and analyzing numerical data to identify patterns, trends, and statistical relationships.  

Let’s explore their key differences: 

Nature of Data:

• Quantitative research : Involves numerical data that can be measured and analyzed statistically.
• Qualitative research : Focuses on non-numerical data, such as words, images, and observations, to capture subjective experiences and meanings.

Research Questions:

• Quantitative research : Typically addresses questions related to “how many,” “how much,” or “to what extent,” aiming to quantify relationships and patterns.
• Qualitative research: Explores questions related to “why” and “how,” aiming to understand the underlying motivations, beliefs, and perceptions of individuals.

Data Collection Methods:

• Quantitative research : Relies on structured surveys, experiments, or observations with predefined variables and measures.
• Qualitative research : Utilizes open-ended interviews, focus groups, participant observations, and textual analysis to gather rich, contextually nuanced data.

Analysis Techniques:

• Quantitative research: Involves statistical analysis to identify correlations, associations, or differences between variables.
• Qualitative research: Employs thematic analysis, coding, and interpretation to uncover patterns, themes, and insights within qualitative data.

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• Last modified: January 3, 2023
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Quantitative determination of selected heavy metals and micronutrients in branded dairy products sold in nigeria using atomic absorption spectroscopy (aas), sunday nwankwo okafor, chinenye josse okafor, emmanuella tochukwu ogbonna, chinelo ezenwafor, patience ogoamaka osadebe.

Quantitative analysis of heavy metals and nutrients in food helps indicate the safety and quality of food for final consumers. The present  study was conducted to assess the presence of heavy metals (arsenic, copper, mercury, chromium, and lead) and the nutritional value of  calcium in branded milk and yogurt to evaluate health risks for consumers. Ten (10) samples of branded milk and dairy products  manufactured in Nigeria were purchased. The metal contents of the samples were determined using atomic absorption spectroscopy.  The concentrations of calcium in the milk samples were between 9.33 ± 0.0023 and 18 ± 0.0071 ppm and were detected in all samples.  Arsenic concentrations ranged from 0.45 ± 0.00042 to 2.48 ± 0.00064 ppm in eight branded samples but were undetected in two samples.  Chromium levels were undetected in most samples, except for two with concentrations of 0.12±0.00049 ppm and 0.23±0.00021 ppm, respectively. Copper ranged from 0.032±0.00021 ppm to 0.129±0.00021 ppm in six samples. Mercury levels were detected in six samples  at a concentration of 1.0±1.0 ppm. Lead concentrations ranged from 0.15±0.00064 to 0.29±0.00028 ppm in three samples. The study found heavy metals above the ideal concentration in branded milk and dairy products in Nigeria, highlighting the need for quality control  measures during production to prevent contamination. 

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