null and alternative hypothesis in experimental research

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Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis ( H 0 ): There’s no effect in the population .
• Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

• The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
• The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question.
• They both make claims about the population.
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

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To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
• Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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8.1 – The null and alternative hypotheses

• Introduction

Statistical Inference in the NHST Framework

Nhst workflow, null hypothesis, alternative hypothesis, alternative hypothesis often may be the research hypothesis, how to interpret the results of a statistical test, outcomes of an experiment, chapter 8 contents.

• a calculated test statistic
• degrees of freedom associated with the calculation of the test statistic
• Recall from our previous discussion (Chapter 8.2) that this is not strictly the interpretation of p-value, but a short-hand for how likely the data fit the null hypothesis. P-value alone can’t tell us about “truth.”
• in the event we reject the null hypothesis, we provisionally accept the alternative hypothesis .

By inference we mean to imply some formal process by which a conclusion is reached from data analysis of outcomes of an experiment. The process at its best leads to conclusions based on evidence. In statistics, evidence comes about from the careful and reasoned application of statistical procedures and the evaluation of probability (Abelson 1995).

Formally, statistics is rich in inference process. We begin by defining the  classical frequentist,  aka Neyman-Pearson approach, to inference, which involves the pairing of two kinds of statistical hypotheses: the null hypothesis (H O ) and the alternate hypothesis (H A ). Whether we accept the hull hypothesis or not is evaluated against a decision criterion, a fixed statistical significance level  (Lehmann 1992). Significance level refers to the setting of a  p-value threshold before testing is done. The threshold is often set to Type I error of 5% (Cowles & Davis 1982), but researchers should always consider whether this threshold is appropriate for their work (Benjamin et al 2017).

This inference process is referred to as Null Hypothesis Significance Testing, NHST. Additionally, a probability value will be obtained for the test outcome or test statistic value. In the Fisherian likelihood tradition, the magnitude of this statistic value can be associated with a probability value, the p-value, of how likely the result is given the null hypothesis is “true”. (Again, keep in mind that this is not strictly the interpretation of p-value, it’s a short-hand for how likely the data fit the null hypothesis. P-value alone can’t tell us about “truth”, per our  discussion, Chapter 8.2 .)

About -logP . P-values are traditionally reported as a decimal, like 0.000134, in the closed (set) interval (0,1) — p-values can never be exactly zero or one. The smaller the value, the less the chance our data agree with the null prediction. Small numbers like this can be confusing, particularly if many p-values are reported, like in many genomics works, e.g., GWAS studies. Instead of reporting vanishingly small p-values, studies may report the negative log 10 p-value , or -logP . Instead of small numbers, large numbers are reported, the larger, the more against the null hypothesis. Thus, our p-value becomes 3.87 -logP.

Why log 10 and not some other base transform? Just that log 10 is convenience — powers of 10.

The antilog of 3.87 returns our p-value

For convenience, a partial p-value -logP transform table

On your own, complete the table up to -logP 5 – 10. See Question 7 below .

We presented in the introduction to Chapter 8 without discussion a simple flow chart to illustrate the process of decision (Figure 1). Here, we repeat the flow chart diagram and follow with descriptions of the elements.

Figure 1. Flow chart of inductive statistical reasoning.

What’s missing from the flow chart is the very necessary caveat that interpretation of the null hypothesis is associated with two kinds of error, Type I error and Type II error. These points and others are discussed in the following sections.

We start with the hypothesis statements. For illustration we discuss hypotheses in terms of comparisons involving just two groups, also called two sample tests . One sample tests in contrast refer to scenarios where you compare a sample statistic to a population value. Extending these concepts to more than two samples is straight-forward, but we leave that discussion to Chapters 12 – 18.

By far the most common application of the null hypothesis testing paradigm involves the comparisons of different treatment groups on some outcome variable. These kinds of null hypotheses are the subject of Chapters 8 through 12.

The  Null hypothesis  (H O ) is a statement about the comparisons, e.g., between a sample statistic and the population, or between two treatment groups. The former is referred to as a one tailed test whereas the latter is called a two-tailed test . The null hypothesis is typically “no statistical difference” between the comparisons.

For example, a one sample, two tailed null hypothesis.

and we read it as “there is no statistical difference between our sample mean and the population mean.” For the more likely case in which no population mean is available, we provide another example, a two sample, two tailed null hypothesis.

Here, we read the statement as “there is no difference between our two sample means.” Equivalently, we interpret the statement as both sample means estimate the same population mean.

Under the Neyman-Pearson approach to inference we have two hypotheses: the null hypothesis and the alternate hypothesis. The hull hypothesis was defined above.

Tails of a test are discussed further in chapter 8.4 .

Alternative hypothesis  (H A ): If we conclude that the null hypothesis is false, or rather and more precisely, we find that we provisionally fail to reject the null hypothesis, then we provisionally accept the alternative hypothesis . The view then is that something other than random chance has influenced the sample observations. Note that the pairing of null and alternative hypotheses covers all possible outcomes. We do not, however, say that we have evidence for the alternative hypothesis under this statistical regimen (Abelson 1995). We tested the null hypothesis, not the alternative hypothesis. Thus, it is incorrect to write that, having found a statistical difference between two drug treatments, say aspirin and acetaminophen for relief of migraine symptoms, it is not correct to conclude that we have proven the case that acetaminophen improves improves symptoms of migraine sufferers.

For the one sample, two tailed null hypothesis, the alternative hypothesis is

and we read it as “there is a statistical difference between our sample mean and the population mean.” For the two sample, two tailed null hypothesis, the alternative hypothesis would be

and we read it as “there is a statistical difference between our two sample means.”

It may be helpful to distinguish between technical hypotheses, scientific hypothesis, or the equality of different kinds of treatments. Tests of technical hypotheses include the testing of statistical assumptions like normality assumption (see Chapter 13.3 ) and homogeneity of variances ( Chapter 13.4 ). The results of inferences about technical hypotheses are used by the statistician to justify selection of parametric statistical tests ( Chapter 13 ). The testing of some scientific hypothesis like whether or not there is a positive link between lifespan and insulin-like growth factor levels in humans (Fontana et al 2008), like the link between lifespan and IGFs in other organisms (Holtzenberger et al 2003), can be further advanced by considering multiple hypotheses and a test of nested hypotheses and evaluated either in Bayesian or likelihood approaches ( Chapter 16 and Chapter 17 ).

Any number of statistical tests may be used to calculate the value of the  test statistic . For example, a one sample t-test may be used to evaluate the difference between the sample mean and the population mean ( Chapter 8.5 ) or the independent sample t-test may be used to evaluate the difference between means of the control group and the treatment group ( Chapter 10 ). The test statistic is the particular value of the outcome of our evaluation of the hypothesis and it is associated with the p-value. In other words, given the assumption of a particular probability distribution, in this case the t-distribution, we can associate a probability, the p-value, that we observed the particular value of the test statistic and the null hypothesis is true in the reference population.

By convention, we determine  statistical significance  (Cox 1982; Whitley & Ball 2002) by assigning ahead of time a decision probability called the  Type I error rate , often given the symbol  α  (alpha). The practice is to look up the  critical value  that corresponds to the outcome of the test with degrees of freedom like your experiment and at the Type I error rate that you selected. The  Degrees of Freedom  (DF, df, or sometimes noted by the symbol  v ), are the number of independent pieces of information available to you. Knowing the degrees of freedom is a crucial piece of information for making the correct tests. Each statistical test has a specific formula for obtaining the independent information available for the statistical test. We first were introduced to DF when we calculated the sample variance with the Bessel correction , n – 1, instead of dividing through by n. With the df in hand, the value of the test statistic is compared to the critical value for our null hypothesis. If the test statistic is smaller than the critical value, we fail to reject the null hypothesis. If, however, the test statistic is greater than the critical value, then we provisionally reject the null hypothesis. This critical value comes from a probability distribution appropriate for the kind of sampling and properties of the measurement we are using. In other words, the rejection criterion for the null hypothesis is set to a critical value, which corresponds to a known probability, the Type I error rate.

Before proceeding with yet another interpretation, and hopefully less technical discussion about test statistics and critical values, we need to discuss the two types of statistical errors. The Type I error rate is the statistical error assigned to the probability that we may reject a null hypothesis as a result of our evaluation of our data when in fact in the reference population, the null hypothesis is, in fact, true. In Biology we generally use Type I error α = 0.05 level of significance. We say that the probability of obtaining the observed value AND H O  is true is 1 in 20 (5%) if α = 0.05. Put another way, we are willing to reject the Null Hypothesis when there is only a 5% chance that the observations could occur and the Null hypothesis is still true. Our test statistic is associated with the p-value; the critical value is associated with the Type I error rate. If and only if the test statistic value equals the critical value will the p-value equal the Type I error rate.

The second error type associated with hypothesis testing is, β, the  Type II statistical error rate . This is the case where we accept or fail to reject a null hypothesis based on our data, but in the reference population, the situation is that indeed, the null hypothesis is actually false.

Thus, we end with a concept that may take you a while to come to terms with — there are four, not two possible outcomes of an experiment.

What are the possible outcomes of a comparative experiment\? We have two treatments, one in which subjects are given a treatment and the other, subjects receive a placebo. Subjects are followed and an outcome is measured. We calculate the descriptive statistics aka summary statistics, means, standard deviations, and perhaps other statistics, and then ask whether there is a difference between the statistics for the groups. So, two possible outcomes of the experiment, correct\? If the treatment has no effect, then we would expect the two groups to have roughly the same values for means, etc., in other words, any difference between the groups is due to chance fluctuations in the measurements and not because of any systematic effect due to the treatment received. Conversely, then if there is a difference due to the treatment, we expect to see a large enough difference in the statistics so that we would notice the systematic effect due to the treatment.

Actually, there are four, not two, possible outcomes of an experiment, just as there were four and not two conclusions about the results of a clinical assay. The four possible outcomes of a test of a statistical null hypothesis are illustrated in Table 1.

Table 1. When conducting hypothesis testing, four outcomes are possible.

In the actual population, a thing happens or it doesn’t. The null hypothesis is either true or it is not. But we don’t have access to the reference population, we don’t have a census. In other words, there is truth, but we don’t have access to the truth. We can weight, assigned as a probability or p-value,  our decisions by how likely our results are given the assumption that the truth is indeed “no difference.”

If you recall, we’ve seen a table like Table 1 before in our discussion of conditional probability and risk analysis ( Chapter 7.3 ). We made the point that statistical inference and the interpretation of clinical tests are similar (Browner and Newman 1987). From the perspective of ordering a diagnostic test , the proper null hypothesis would be the patient does not have the disease. For your review, here’s that table (Table 2).

Table 2. Interpretations of results of a diagnostic or clinical test.

Thus, a positive diagnostic test result is interpreted as rejecting the null hypothesis. If the person actually does not have the disease, then the positive diagnostic test is a false positive.

• Match the corresponding entries in the two tables. For example, which outcome from the inference/hypothesis table matches  specificity of the test\ ?
• Find three sources on the web for definitions of the p-value. Write out these definitions in your notes and compare them.
• In your own words distinguish between the test statistic and the critical value.
• Can the p-value associated with the test statistic ever be zero\? Explain.
• Since the p-value is associated with the test statistic and the null hypothesis is true, what value must the p-value be for us to provisionally reject the null hypothesis\?
• All of our discussions have been about testing the null hypothesis, about accepting or rejecting, provisionally, the null hypothesis. If we reject the null hypothesis, can we say that we have evidence for the alternate hypothesis\?
• What are the p-values for -logP of 5, 6, 7, 8, 9, and 10\? Complete the p-value -logP transform table .
• Instead of log 10 transform, create a similar table but for negative natural log transform. Which is more convenient? Hint: log(x, base=exp(1))
• The null and alternative hypotheses
• The controversy over proper hypothesis testing
• Sampling distribution and hypothesis testing
• Tails of a test
• One sample t-test
• Confidence limits for the estimate of population mean
• References and suggested readings

Microbe Notes

Null hypothesis and alternative hypothesis with 9 differences

Table of Contents

Interesting Science Videos

Null hypothesis definition

The null hypothesis is a general statement that states that there is no relationship between two phenomenons under consideration or that there is no association between two groups.

• A hypothesis, in general, is an assumption that is yet to be proved with sufficient pieces of evidence. A null hypothesis thus is the hypothesis a researcher is trying to disprove.
• A null hypothesis is a hypothesis capable of being objectively verified, tested, and even rejected.
• If a study is to compare method A with method B about their relationship, and if the study is preceded on the assumption that both methods are equally good, then this assumption is termed as the null hypothesis.
• The null hypothesis should always be a specific hypothesis, i.e., it should not state about or approximately a certain value.

Null hypothesis symbol

• The symbol for the null hypothesis is H 0, and it is read as H-null, H-zero, or H-naught.
• The null hypothesis is usually associated with just ‘equals to’ sign as a null hypothesis can either be accepted or rejected.

Null hypothesis purpose

• The main purpose of a null hypothesis is to verify/ disprove the proposed statistical assumptions.
• Some scientific null hypothesis help to advance a theory.
• The null hypothesis is also used to verify the consistent results of multiple experiments. For e.g., the null hypothesis stating that there is no relation between some medication and age of the patients supports the general effectiveness conclusion, and allows recommendations.

Null hypothesis principle

• The principle of the null hypothesis is collecting the data and determining the chances of the collected data in the study of a random sample, proving that the null hypothesis is true.
• In situations or studies where the collected data doesn’t complete the expectation of the null hypothesis, it is concluded that the data doesn’t provide sufficient or reliable pieces of evidence to support the null hypothesis and thus, it is rejected.
• The data collected is tested through some statistical tool which is designed to measure the extent of departure of the date from the null hypothesis.
• The procedure decides whether the observed departure obtained from the statistical tool is larger than a defined value so that the probability of occurrence of a high departure value is very small under the null hypothesis.
• However, some data might not contradict the null hypothesis which explains that only a weak conclusion can be made and that the data doesn’t provide strong pieces of evidence against the null hypothesis and the null hypothesis might or might not be true.
• Under some other conditions, if the data collected is sufficient and is capable of providing enough evidence, the null hypothesis can be considered valid, indicating no relationship between the phenomena.

When to reject null hypothesis?

• When the p-value of the data is less than the significant level of the test, the null hypothesis is rejected, indicating the test results are significant.
• However, if the p-value is higher than the significant value, the null hypothesis is not rejected, and the results are considered not significant.
• The level of significance is an important concept while hypothesis testing as it determines the percentage risk of rejecting the null hypothesis when H 0 might happen to be true.
• In other words, if we take the level of significance at 5%, it means that the researcher is willing to take as much as a 5 percent risk of rejecting the null hypothesis when it (H 0 ) happens to be true.
• The null hypothesis cannot be accepted because the lack of evidence only means that the relationship is not proven. It doesn’t prove that something doesn’t exist, but it just means that there are not enough shreds of evidence and the study might have missed it.

Null hypothesis examples

The following are some examples of null hypothesis:

• If the hypothesis is that “the consumption of a particular medicine reduces the chances of heart arrest”, the null hypothesis will be “the consumption of the medicine doesn’t reduce the chances of heart arrest.”
• If the hypothesis is that, “If random test scores are collected from men and women, does the score of one group differ from the other?” a possible null hypothesis will be that the mean test score of men is the same as that of the women.

H 0 : µ 1 = µ 2

H 0 = null hypothesis µ 1 = mean score of men µ 2 = mean score of women

Alternative hypothesis definition

An alternative hypothesis is a statement that describes that there is a relationship between two selected variables in a study.

• An alternative hypothesis is usually used to state that a new theory is preferable to the old one (null hypothesis).
• This hypothesis can be simply termed as an alternative to the null hypothesis.
• The alternative hypothesis is the hypothesis that is to be proved that indicates that the results of a study are significant and that the sample observation is not results just from chance but from some non-random cause.
• If a study is to compare method A with method B about their relationship and we assume that the method A is superior or the method B is inferior, then such a statement is termed as an alternative hypothesis.
• Alternative hypotheses should be clearly stated, considering the nature of the research problem.

Alternative hypothesis symbol

• The symbol of the alternative hypothesis is either H 1 or H a while using less than, greater than or not equal signs.

Alternative hypothesis purpose

• An alternative hypothesis provides the researchers with some specific restatements and clarifications of the research problem.
• An alternative hypothesis provides a direction to the study, which then can be utilized by the researcher to obtain the desired results.
• Since the alternative hypothesis is selected before conducting the study, it allows the test to prove that the study is supported by evidence, separating it from the researchers’ desires and values.
• An alternative hypothesis provides a chance of discovering new theories that can disprove an existing one that might not be supported by evidence.
• The alternative hypothesis is important as they prove that a relationship exists between two variables selected and that the results of the study conducted are relevant and significant.

Alternative hypothesis principle

• The principle behind the alternative hypothesis is similar to that of the null hypothesis.
• The alternative hypothesis is based on the concept that when sufficient evidence is collected from the data of random sample, it provides a basis for proving the assumption made by the researcher regarding the study.
• Like in the null hypothesis, the data collected from a random sample is passed through a statistical tool that measures the extent of departure of the data from the null hypothesis.
• If the departure is small under the selected level of significance, the alternative hypothesis is accepted, and the null hypothesis is rejected.
• If the data collected don’t have chances of being in the study of the random sample and are instead decided by the relationship within the sample of the study, an alternative hypothesis stands true.

Alternative hypothesis examples

The following are some examples of alternative hypothesis:

1. If a researcher is assuming that the bearing capacity of a bridge is more than 10 tons, then the hypothesis under this study will be:

Null hypothesis H 0 : µ= 10 tons Alternative hypothesis H a : µ>10 tons

2. Under another study that is trying to test whether there is a significant difference between the effectiveness of medicine against heart arrest, the alternative hypothesis will be that there is a relationship between the medicine and chances of heart arrest.

Null hypothesis vs Alternative hypothesis

• R. Kothari (1990) Research Methodology. Vishwa Prakasan. India.
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• https://byjus.com/maths/null-hypothesis/
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• 1% – https://www.thoughtco.com/definition-of-null-hypothesis-and-examples-605436
• 1% – https://www.quora.com/What-are-the-different-types-of-hypothesis-and-what-are-some-examples-of-them
• 1% – https://www.dummies.com/education/math/statistics/what-a-p-value-tells-you-about-statistical-data/
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Null Hypothesis and Alternative Hypothesis

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Hypothesis testing involves the careful construction of two statements: the null hypothesis and the alternative hypothesis. These hypotheses can look very similar but are actually different.

How do we know which hypothesis is the null and which one is the alternative? We will see that there are a few ways to tell the difference.

The Null Hypothesis

The null hypothesis reflects that there will be no observed effect in our experiment. In a mathematical formulation of the null hypothesis, there will typically be an equal sign. This hypothesis is denoted by H 0 .

The null hypothesis is what we attempt to find evidence against in our hypothesis test. We hope to obtain a small enough p-value that it is lower than our level of significance alpha and we are justified in rejecting the null hypothesis. If our p-value is greater than alpha, then we fail to reject the null hypothesis.

If the null hypothesis is not rejected, then we must be careful to say what this means. The thinking on this is similar to a legal verdict. Just because a person has been declared "not guilty", it does not mean that he is innocent. In the same way, just because we failed to reject a null hypothesis it does not mean that the statement is true.

For example, we may want to investigate the claim that despite what convention has told us, the mean adult body temperature is not the accepted value of 98.6 degrees Fahrenheit . The null hypothesis for an experiment to investigate this is “The mean adult body temperature for healthy individuals is 98.6 degrees Fahrenheit.” If we fail to reject the null hypothesis, then our working hypothesis remains that the average adult who is healthy has a temperature of 98.6 degrees. We do not prove that this is true.

If we are studying a new treatment, the null hypothesis is that our treatment will not change our subjects in any meaningful way. In other words, the treatment will not produce any effect in our subjects.

The Alternative Hypothesis

The alternative or experimental hypothesis reflects that there will be an observed effect for our experiment. In a mathematical formulation of the alternative hypothesis, there will typically be an inequality, or not equal to symbol. This hypothesis is denoted by either H a or by H 1 .

The alternative hypothesis is what we are attempting to demonstrate in an indirect way by the use of our hypothesis test. If the null hypothesis is rejected, then we accept the alternative hypothesis. If the null hypothesis is not rejected, then we do not accept the alternative hypothesis. Going back to the above example of mean human body temperature, the alternative hypothesis is “The average adult human body temperature is not 98.6 degrees Fahrenheit.”

If we are studying a new treatment, then the alternative hypothesis is that our treatment does, in fact, change our subjects in a meaningful and measurable way.

The following set of negations may help when you are forming your null and alternative hypotheses. Most technical papers rely on just the first formulation, even though you may see some of the others in a statistics textbook.

• Null hypothesis: “ x is equal to y .” Alternative hypothesis “ x is not equal to y .”
• Null hypothesis: “ x is at least y .” Alternative hypothesis “ x is less than y .”
• Null hypothesis: “ x is at most y .” Alternative hypothesis “ x is greater than y .”
• What 'Fail to Reject' Means in a Hypothesis Test
• Type I and Type II Errors in Statistics
• An Example of a Hypothesis Test
• The Runs Test for Random Sequences
• An Example of Chi-Square Test for a Multinomial Experiment
• The Difference Between Type I and Type II Errors in Hypothesis Testing
• What Level of Alpha Determines Statistical Significance?
• What Is the Difference Between Alpha and P-Values?
• What Is ANOVA?
• How to Find Critical Values with a Chi-Square Table
• Example of a Permutation Test
• Degrees of Freedom for Independence of Variables in Two-Way Table
• Example of an ANOVA Calculation
• How to Find Degrees of Freedom in Statistics
• How to Construct a Confidence Interval for a Population Proportion
• Degrees of Freedom in Statistics and Mathematics

• Key Differences

Know the Differences & Comparisons

Difference Between Null and Alternative Hypothesis

Null hypothesis implies a statement that expects no difference or effect. On the contrary, an alternative hypothesis is one that expects some difference or effect. Null hypothesis This article excerpt shed light on the fundamental differences between null and alternative hypothesis.

Content: Null Hypothesis Vs Alternative Hypothesis

Comparison chart.

Definition of Null Hypothesis

A null hypothesis is a statistical hypothesis in which there is no significant difference exist between the set of variables. It is the original or default statement, with no effect, often represented by H 0 (H-zero). It is always the hypothesis that is tested. It denotes the certain value of population parameter such as µ, s, p. A null hypothesis can be rejected, but it cannot be accepted just on the basis of a single test.

Definition of Alternative Hypothesis

A statistical hypothesis used in hypothesis testing, which states that there is a significant difference between the set of variables. It is often referred to as the hypothesis other than the null hypothesis, often denoted by H 1 (H-one). It is what the researcher seeks to prove in an indirect way, by using the test. It refers to a certain value of sample statistic, e.g., x¯, s, p

The acceptance of alternative hypothesis depends on the rejection of the null hypothesis i.e. until and unless null hypothesis is rejected, an alternative hypothesis cannot be accepted.

Key Differences Between Null and Alternative Hypothesis

The important points of differences between null and alternative hypothesis are explained as under:

• A null hypothesis is a statement, in which there is no relationship between two variables. An alternative hypothesis is a statement; that is simply the inverse of the null hypothesis, i.e. there is some statistical significance between two measured phenomenon.
• A null hypothesis is what, the researcher tries to disprove whereas an alternative hypothesis is what the researcher wants to prove.
• A null hypothesis represents, no observed effect whereas an alternative hypothesis reflects, some observed effect.
• If the null hypothesis is accepted, no changes will be made in the opinions or actions. Conversely, if the alternative hypothesis is accepted, it will result in the changes in the opinions or actions.
• As null hypothesis refers to population parameter, the testing is indirect and implicit. On the other hand, the alternative hypothesis indicates sample statistic, wherein, the testing is direct and explicit.
• A null hypothesis is labelled as H 0 (H-zero) while an alternative hypothesis is represented by H 1 (H-one).
• The mathematical formulation of a null hypothesis is an equal sign but for an alternative hypothesis is not equal to sign.
• In null hypothesis, the observations are the outcome of chance whereas, in the case of the alternative hypothesis, the observations are an outcome of real effect.

There are two outcomes of a statistical test, i.e. first, a null hypothesis is rejected and alternative hypothesis is accepted, second, null hypothesis is accepted, on the basis of the evidence. In simple terms, a null hypothesis is just opposite of alternative hypothesis.

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Zipporah Thuo says

February 22, 2018 at 6:06 pm

The comparisons between the two hypothesis i.e Null hypothesis and the Alternative hypothesis are the best.Thank you.

Getu Gamo says

March 4, 2019 at 3:42 am

Thank you so much for the detail explanation on two hypotheses. Now I understood both very well, including their differences.

Jyoti Bhardwaj says

May 28, 2019 at 6:26 am

Thanks, Surbhi! Appreciate the clarity and precision of this content.

January 9, 2020 at 6:16 am

John Jenstad says

July 20, 2020 at 2:52 am

Thanks very much, Surbhi, for your clear explanation!!

Navita says

July 2, 2021 at 11:48 am

Thanks for the Comparison chart! it clears much of my doubt.

GURU UPPALA says

July 21, 2022 at 8:36 pm

Thanks for the Comparison chart!

Enock kipkoech says

September 22, 2022 at 1:57 pm

What are the examples of null hypothesis and substantive hypothesis

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What is The Null Hypothesis & When Do You Reject The Null Hypothesis

Julia Simkus

Editor at Simply Psychology

BA (Hons) Psychology, Princeton University

Julia Simkus is a graduate of Princeton University with a Bachelor of Arts in Psychology. She is currently studying for a Master's Degree in Counseling for Mental Health and Wellness in September 2023. Julia's research has been published in peer reviewed journals.

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Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Associate Editor for Simply Psychology

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On This Page:

A null hypothesis is a statistical concept suggesting no significant difference or relationship between measured variables. It’s the default assumption unless empirical evidence proves otherwise.

The null hypothesis states no relationship exists between the two variables being studied (i.e., one variable does not affect the other).

The null hypothesis is the statement that a researcher or an investigator wants to disprove.

Testing the null hypothesis can tell you whether your results are due to the effects of manipulating ​ the dependent variable or due to random chance. 

How to Write a Null Hypothesis

Null hypotheses (H0) start as research questions that the investigator rephrases as statements indicating no effect or relationship between the independent and dependent variables.

It is a default position that your research aims to challenge or confirm.

For example, if studying the impact of exercise on weight loss, your null hypothesis might be:

There is no significant difference in weight loss between individuals who exercise daily and those who do not.

Examples of Null Hypotheses

When Do We Reject The Null Hypothesis? 

We reject the null hypothesis when the data provide strong enough evidence to conclude that it is likely incorrect. This often occurs when the p-value (probability of observing the data given the null hypothesis is true) is below a predetermined significance level.

If the collected data does not meet the expectation of the null hypothesis, a researcher can conclude that the data lacks sufficient evidence to back up the null hypothesis, and thus the null hypothesis is rejected. 

Rejecting the null hypothesis means that a relationship does exist between a set of variables and the effect is statistically significant ( p > 0.05).

If the data collected from the random sample is not statistically significance , then the null hypothesis will be accepted, and the researchers can conclude that there is no relationship between the variables. 

You need to perform a statistical test on your data in order to evaluate how consistent it is with the null hypothesis. A p-value is one statistical measurement used to validate a hypothesis against observed data.

Calculating the p-value is a critical part of null-hypothesis significance testing because it quantifies how strongly the sample data contradicts the null hypothesis.

The level of statistical significance is often expressed as a  p  -value between 0 and 1. The smaller the p-value, the stronger the evidence that you should reject the null hypothesis.

Usually, a researcher uses a confidence level of 95% or 99% (p-value of 0.05 or 0.01) as general guidelines to decide if you should reject or keep the null.

When your p-value is less than or equal to your significance level, you reject the null hypothesis.

In other words, smaller p-values are taken as stronger evidence against the null hypothesis. Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis.

In this case, the sample data provides insufficient data to conclude that the effect exists in the population.

Because you can never know with complete certainty whether there is an effect in the population, your inferences about a population will sometimes be incorrect.

When you incorrectly reject the null hypothesis, it’s called a type I error. When you incorrectly fail to reject it, it’s called a type II error.

Why Do We Never Accept The Null Hypothesis?

The reason we do not say “accept the null” is because we are always assuming the null hypothesis is true and then conducting a study to see if there is evidence against it. And, even if we don’t find evidence against it, a null hypothesis is not accepted.

A lack of evidence only means that you haven’t proven that something exists. It does not prove that something doesn’t exist. 

It is risky to conclude that the null hypothesis is true merely because we did not find evidence to reject it. It is always possible that researchers elsewhere have disproved the null hypothesis, so we cannot accept it as true, but instead, we state that we failed to reject the null. 

One can either reject the null hypothesis, or fail to reject it, but can never accept it.

Why Do We Use The Null Hypothesis?

We can never prove with 100% certainty that a hypothesis is true; We can only collect evidence that supports a theory. However, testing a hypothesis can set the stage for rejecting or accepting this hypothesis within a certain confidence level.

The null hypothesis is useful because it can tell us whether the results of our study are due to random chance or the manipulation of a variable (with a certain level of confidence).

A null hypothesis is rejected if the measured data is significantly unlikely to have occurred and a null hypothesis is accepted if the observed outcome is consistent with the position held by the null hypothesis.

Rejecting the null hypothesis sets the stage for further experimentation to see if a relationship between two variables exists. 

Hypothesis testing is a critical part of the scientific method as it helps decide whether the results of a research study support a particular theory about a given population. Hypothesis testing is a systematic way of backing up researchers’ predictions with statistical analysis.

It helps provide sufficient statistical evidence that either favors or rejects a certain hypothesis about the population parameter. 

Purpose of a Null Hypothesis 

• The primary purpose of the null hypothesis is to disprove an assumption. 
• Whether rejected or accepted, the null hypothesis can help further progress a theory in many scientific cases.
• A null hypothesis can be used to ascertain how consistent the outcomes of multiple studies are.

Do you always need both a Null Hypothesis and an Alternative Hypothesis?

The null (H0) and alternative (Ha or H1) hypotheses are two competing claims that describe the effect of the independent variable on the dependent variable. They are mutually exclusive, which means that only one of the two hypotheses can be true. 

While the null hypothesis states that there is no effect in the population, an alternative hypothesis states that there is statistical significance between two variables. 

The goal of hypothesis testing is to make inferences about a population based on a sample. In order to undertake hypothesis testing, you must express your research hypothesis as a null and alternative hypothesis. Both hypotheses are required to cover every possible outcome of the study. 

What is the difference between a null hypothesis and an alternative hypothesis?

The alternative hypothesis is the complement to the null hypothesis. The null hypothesis states that there is no effect or no relationship between variables, while the alternative hypothesis claims that there is an effect or relationship in the population.

It is the claim that you expect or hope will be true. The null hypothesis and the alternative hypothesis are always mutually exclusive, meaning that only one can be true at a time.

What are some problems with the null hypothesis?

One major problem with the null hypothesis is that researchers typically will assume that accepting the null is a failure of the experiment. However, accepting or rejecting any hypothesis is a positive result. Even if the null is not refuted, the researchers will still learn something new.

Why can a null hypothesis not be accepted?

We can either reject or fail to reject a null hypothesis, but never accept it. If your test fails to detect an effect, this is not proof that the effect doesn’t exist. It just means that your sample did not have enough evidence to conclude that it exists.

We can’t accept a null hypothesis because a lack of evidence does not prove something that does not exist. Instead, we fail to reject it.

Failing to reject the null indicates that the sample did not provide sufficient enough evidence to conclude that an effect exists.

If the p-value is greater than the significance level, then you fail to reject the null hypothesis.

Is a null hypothesis directional or non-directional?

A hypothesis test can either contain an alternative directional hypothesis or a non-directional alternative hypothesis. A directional hypothesis is one that contains the less than (“<“) or greater than (“>”) sign.

A nondirectional hypothesis contains the not equal sign (“≠”).  However, a null hypothesis is neither directional nor non-directional.

A null hypothesis is a prediction that there will be no change, relationship, or difference between two variables.

The directional hypothesis or nondirectional hypothesis would then be considered alternative hypotheses to the null hypothesis.

Gill, J. (1999). The insignificance of null hypothesis significance testing.  Political research quarterly ,  52 (3), 647-674.

Krueger, J. (2001). Null hypothesis significance testing: On the survival of a flawed method.  American Psychologist ,  56 (1), 16.

Masson, M. E. (2011). A tutorial on a practical Bayesian alternative to null-hypothesis significance testing.  Behavior research methods ,  43 , 679-690.

Nickerson, R. S. (2000). Null hypothesis significance testing: a review of an old and continuing controversy.  Psychological methods ,  5 (2), 241.

Rozeboom, W. W. (1960). The fallacy of the null-hypothesis significance test.  Psychological bulletin ,  57 (5), 416.

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Difference between Null and Alternate Hypothesis

Hypothesis is a statement or an assumption that may be true or false. There are six types of hypotheses mainly the Simple hypothesis, Complex hypothesis, Directional hypothesis, Associative hypothesis, and Null hypothesis. Usually, the hypothesis is the start point of any scientific investigation, It gives the right direction to the process of investigation. It avoids the blind search and gives direction to the search. It acts as a compass in the process.

Null hypothesis suggests that there is no relationship between the two variables. Null hypothesis is also exactly the opposite of the alternative hypothesis. Null hypothesis is generally what researchers or scientists try to disprove and if the null hypothesis gets accepted then we have to make changes in our opinion i.e. we have to make changes in our original opinion or statement in order to match null hypothesis. Null hypothesis is represented as H0. If my alternative hypothesis is that 55% of boys in my town are taller than girls then my alternative hypothesis will be that 55% of boys in my town are not taller than girls. 

Alternative hypothesis is a method for reaching a conclusion and making inferences and judgements about certain facts or a statement. This is done on the basis of the data which is available. Usually, the statement which we check regarding the null hypothesis is commonly known as the alternative hypothesis. Most of the times alternative hypothesis is exactly the opposite of the null hypothesis. This is what generally researchers or scientists try to approve. Alternative hypothesis is represented as Ha or H1. If my null hypothesis is that 55% of boys in my town are not taller than girls then my alternative hypothesis will be that 55% of boys in my town are taller than girls.

Following is the difference between the null hypothesis and alternate hypothesis:

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Null vs Alternative hypothesis in practice

From the beginning of the most introductory statistics course, the declaration of a null and alternative hypothesis is the "first step" of any good experiment and subsequent analysis. Now that I have been venturing into more complex courses and topics, I see this exercise still being performed. I have always perceived the proposal of the null v. alternative as a teachable example of how to think of a study or experiment rather than a necessity (once you have an understanding of hypothesis testing and experimental design).

Do statisticians consistently propose a null and alternative in practice for all tests they perform? Is this common practice in the field or within a company? Or rather is it a "mental" heuristic when approaching statistical testing?

From my time doing experiments in biological research, we have always had a hypothesis to guide and motivate an experiment, but there was never the clear definition of a null and alternative. Is this an example of lack of scientific rigor?

Sorry for the naiveté of question as I am not a statistician.

• hypothesis-testing

• 1 $\begingroup$ Although it's unclear what you mean by "propose these hypotheses," perhaps it's relevant to know there is a theoretical statistical literature that provides clear, rigorous definitions of null and alternative hypotheses. In that literature, the null must imply a definite distribution of a test statistic. In all but the simplest cases (consisting of a "simple null" and "simple alternative"), that uniquely determines the null. Any lack of rigor, then, must originate within the intro stats courses to which you refer, not in the discipline itself. $\endgroup$ –  whuber ♦ Commented Jun 7, 2023 at 19:24
• $\begingroup$ In light of that, perhaps one of these posts about null hypotheses answers your questions. The thread at stats.stackexchange.com/questions/31 comes particularly to mind, because many of the answers there refer to null hypotheses and attempt to characterize them. Please check it out. $\endgroup$ –  whuber ♦ Commented Jun 7, 2023 at 19:25
• 2 $\begingroup$ It’s so common to test a null hypothesis of no effect against an alternative of some effect that it’s kind of a slang to leave that implicit. (Whether or not this is good for the field or science in general is a different matter.) $\endgroup$ –  Dave Commented Jun 7, 2023 at 19:34
• 1 $\begingroup$ While the null and alternative hypothesis has a strong theoretical basis - its use is in fact quite extensive in the real world. In A.H. Studenmund's Using Econometrics: A Practical Guide - the author explains how the FDA tests new products before bringing them to market. For instance, if side effects were observed more frequently then would be expected by chance, then the product would be withheld. Under such a scenario, there would be a difference between the two groups (what would typically be observed and what is actually observed), and the null would be rejected in this scenario. $\endgroup$ –  Michael Grogan Commented Jun 7, 2023 at 19:39
• 4 $\begingroup$ "From my time doing experiments in biological research, we have always had a hypothesis to guide and motivate an experiment, [...]". It is worth remembering a scientific / research hypothesis is different from a statistical hypothesis. The individual concepts are probably rigorously studied, though there is probably a weaker link in between (e.g., coming up with a statistical hypothesis based on the research hypothesis) in practice, leading to concepts like Type III error . $\endgroup$ –  B.Liu Commented Jun 7, 2023 at 20:18

3 Answers 3

Your question starts out as if the statistical null and alternative hypotheses are what you are interested in, but the penultimate sentence makes me think that you might be more interested in the difference between scientific and statistical hypotheses.

Statistical hypotheses can only be those that are expressible within a statistical model. They typically concern values of parameters within the statistical model. Scientific hypotheses almost invariably concern the real world, and they often do not directly translate into the much more limited universe of the chosen statistical model. Few introductory stats books spend any real time considering what constitutes a statistical model (it can be very complicated) and the trivial examples used have scientific hypotheses so simple that the distinction between model and real-world hypotheses is blurry.

I have written an extensive account of hypothesis and significance testing that includes several sections dealing with the distinction between scientific and statistical hypotheses, as well as the dangers that might come from assuming a match between the statistical model and the real-world scientific concerns: A Reckless Guide to P-values

So, to answer your explicit questions:

• No, statisticians do not always use null and alternative hypotheses. Many statistical methods do not require them.

• It is common practice in some disciplines (and maybe some schools of statistics) to specify the null and alternative hypothesis when a hypothesis test is being used. However, you should note that a hypotheses test requires an explicit alternative for the planning stage (e.g. for sample size determination) but once the data are in hand that alternative is no longer relevant. Many times the post-data alternative can be no more than 'not the null'.

• I'm not sure of the mental heuristic thing, but it does seem possible to me that the beginner courses omit so much detail in the service of simplicity that the word 'hypothesis' loses its already vague meaning.

the declaration of a null and alternative hypothesis is the "first step" of any good experiment and subsequent analysis.

Well, you did put quotes around first step, but I'd say the first step in an experiment is figuring out what you want to figure out.

As to "subsequent analysis", it might even be that the subsequent analysis does not involve testing a hypothesis! Maybe you just want to estimate a parameter. Personally, I think tests are overused.

Often, you know in advance that the null is false and you just want to see what is actually going on.

A null hypothesis is not meaningful in every case where statistics are used. It is useful for questions of the type "Do this effect a measurable change on something?"

But if I want to see "how far can I consistently throw a frisbee", there is no null hypothesis. There is still statistics; do a lot of throws, average distances, until I can say with 95% confidence that I can indeed throw the frisbee this far.

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Null Hypothesis

The null hypothesis, H 0 , is an essential part of any research design, and is always tested, even indirectly.

This article is a part of the guide:

• Research Hypothesis
• Defining a Research Problem
• Selecting Method
• Test Hypothesis

Browse Full Outline

• 1 Scientific Method
• 2.1.1 Null Hypothesis
• 2.1.2 Research Hypothesis
• 2.2 Prediction
• 2.3 Conceptual Variable
• 3.1 Operationalization
• 3.2 Selecting Method
• 3.3 Measurements
• 3.4 Scientific Observation
• 4.1 Empirical Evidence
• 5.1 Generalization
• 5.2 Errors in Conclusion

The simplistic definition of the null is as the opposite of the alternative hypothesis , H 1 , although the principle is a little more complex than that.

The null hypothesis (H 0 ) is a hypothesis which the researcher tries to disprove, reject or nullify.

The 'null' often refers to the common view of something, while the alternative hypothesis is what the researcher really thinks is the cause of a phenomenon.

An experiment conclusion always refers to the null, rejecting or accepting H 0 rather than H 1 .

Despite this, many researchers neglect the null hypothesis when testing hypotheses , which is poor practice and can have adverse effects.

Examples of the Null Hypothesis

A researcher may postulate a hypothesis:

H 1 : Tomato plants exhibit a higher rate of growth when planted in compost rather than in soil.

And a null hypothesis:

H 0 : Tomato plants do not exhibit a higher rate of growth when planted in compost rather than soil.

It is important to carefully select the wording of the null, and ensure that it is as specific as possible. For example, the researcher might postulate a null hypothesis:

H 0 : Tomato plants show no difference in growth rates when planted in compost rather than soil.

There is a major flaw with this H 0 . If the plants actually grow more slowly in compost than in soil, an impasse is reached. H 1 is not supported, but neither is H 0 , because there is a difference in growth rates.

If the null is rejected, with no alternative, the experiment may be invalid. This is the reason why science uses a battery of deductive and inductive processes to ensure that there are no flaws in the hypotheses.

Many scientists neglect the null, assuming that it is merely the opposite of the alternative, but it is good practice to spend a little time creating a sound hypothesis. It is not possible to change any hypothesis retrospectively, including H 0 .

Significance Tests

If significance tests generate 95% or 99% likelihood that the results do not fit the null hypothesis, then it is rejected, in favor of the alternative.

Otherwise, the null is accepted. These are the only correct assumptions, and it is incorrect to reject, or accept, H 1 .

Accepting the null hypothesis does not mean that it is true. It is still a hypothesis, and must conform to the principle of falsifiability , in the same way that rejecting the null does not prove the alternative.

Perceived Problems With the Null

The major problem with the H 0 is that many researchers, and reviewers, see accepting the null as a failure of the experiment . This is very poor science, as accepting or rejecting any hypothesis is a positive result.

Even if the null is not refuted, the world of science has learned something new. Strictly speaking, the term ‘failure’, should only apply to errors in the experimental design , or incorrect initial assumptions.

Development of the Null

The Flat Earth model was common in ancient times, such as in the civilizations of the Bronze Age or Iron Age. This may be thought of as the null hypothesis, H 0 , at the time.

H 0 : World is Flat

Many of the Ancient Greek philosophers assumed that the sun, moon and other objects in the universe circled around the Earth. Hellenistic astronomy established the spherical shape of the earth around 300 BC.

H 0 : The Geocentric Model: Earth is the centre of the Universe and it is Spherical

Copernicus had an alternative hypothesis , H 1 that the world actually circled around the sun, thus being the center of the universe. Eventually, people got convinced and accepted it as the null, H 0 .

H 0 : The Heliocentric Model: Sun is the centre of the universe

Later someone proposed an alternative hypothesis that the sun itself also circled around the something within the galaxy, thus creating a new H 0 . This is how research works - the H 0 gets closer to the reality each time, even if it isn't correct, it is better than the last H 0 .

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Martyn Shuttleworth (Feb 3, 2008). Null Hypothesis. Retrieved Sep 01, 2024 from Explorable.com: https://explorable.com/null-hypothesis

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