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Test Cross: An Introduction

A test cross is a cross between a person with an unknown genotype and another person with a homozygous recessive genotype. A test cross determines or discloses the genotype of the original person. A test cross can assist in determining if a dominant phenotype is homozygous or heterozygous for a certain trait.

A test cross is a genetic technique discovered by Gregor Mendel that entails mating an individual with all phenotypically recessive individuals, to ascertain the zygosity of the former by evaluating the proportions of offspring phenotypes. Zygosity can be homozygous or heterozygous. Heterozygous individuals have one dominant and one recessive allele .

Individuals with homozygous dominant alleles have two dominant alleles, whereas individuals with homozygous recessive alleles have two recessive alleles. The alleles inherited from the parents define the genotype of an offspring for each of its genes.

The allele combination is the product of the maternal and paternal chromosomes provided by each gamete during the fertilisation of that offspring. During meiosis in gametes, homologous chromosomes undergo genetic recombination and randomly separate into haploid daughter cells , each with a unique combination of maternally and paternally coded genes. Dominant alleles will overpower recessive allele expression.

The genotypic ratios may be derived using mathematical probability, but the genotypic composition cannot be determined merely by looking at the phenotype of a dominant feature. It is impossible to determine whether a tall plant from F1 or F2 has TT or Tt composition.

As a result, Mendel crossed a tall plant from F2 with a dwarf plant to identify the genotype of a tall plant at F2. It is known as a test cross. Instead of self-pollination, a dominant phenotypic organism is mated with a recessive parent in a normal test cross. The offspring of such a hybrid is simply analysed to predict the genotype of the test organism.

Test Cross Example

Let’s assume that you were handed a tall pea plant with no knowledge of its parentage. Since tallness is a dominant feature in peas, your plant may be homozygous (TT) or heterozygous (Tt), but you'd have no idea. In this case, a test cross can be used to establish its genotype.

If the plant were homozygous (TT), a test cross would produce all tall progeny (TT 🡪 tt: all Tt); if the plant were heterozygous (Tt), the test cross would produce half tall progeny and half short progeny (Tt 🡪 tt: Tt and tt).

Any recessive allele in the unknown genotype is expressed in the progeny of a test cross because it is linked with a recessive allele from the homozygous recessive parent.

Test Cross

What is Homozygous and Heterozygous?

Mendel hypothesised that the allelic pair of genes for height in a pure breeding tall or dwarf pea variety are identical. TT and tt, Bateson and Saunders referred to this situation as “homozygous.” A hybrid is an individual who has two distinct alleles (Tt). This condition was termed heterozygous by Bateson and Saunders.

Individual phenotype, DD and Dd are tall, but dd is a dwarf. When both alleles are the same (DD or dd), the individual is called a homozygote; when the alleles are different (Dd), the words heterozygous and heterozygote are used.

Homozygous Recessive

The organism with an unknown genotype that expresses the dominant phenotype is crossed with a known homozygous recessive individual.

Cross Genetics

Monohybrid Cross

A monohybrid cross is a cross between two individuals who have homozygous genotypes for the opposing phenotype for a certain genetic characteristic. Geneticists utilise monohybrid crosses to study how homozygous children express heterozygous genes acquired from their parents.

Dihybrid Cross

A dihybrid cross is a cross between two distinct genes whose observable features differ. It is the result of a cross between two heterozygous people, for two separate features.

Two lines are crossed to produce a hybrid in a back cross. The offspring are then selected and crossed with one of the parents. Back crosses are extremely beneficial in plant breeding because they allow breeders to hybridise a high-yielding variety with another variety to introduce the desired feature, then back cross to ensure the progeny have the same desirable qualities as the high-yielding variety.

Back Cross

This article covers the test cross, who discovered the test cross and various examples of it. It also stated the difference between homozygous and heterozygous . We have seen a homozygous example and a heterozygous example, and understood the difference between dominant and recessive. Also, how heterozygous recessive can’t show a trait but can become the carrier.

FAQs on Test Cross

1. What is the significance of the test cross?

The significance of the test cross:

Very important in identifying an organism's genetic composition.

Simpler and faster way of obtaining desirable characteristics in homozygous individuals.

Used to establish linkage relationships between genes.

Extremely beneficial to breeders and geneticists.

It aids in determining an organism's genetic make-up.

2. Explain the differences between monohybrid and dihybrid crosses.

A monohybrid cross occurs when two individuals have homozygous genotypes for the opposite phenotype for a certain genetic trait. A dihybrid cross is a cross between two distinct genes whose observable features differ. It is the result of a cross between two people who are heterozygous for two separate features.

The number of characteristics investigated in the offspring distinguishes a monohybrid cross from a dihybrid cross. The inheritance of a single gene is anticipated in a monohybrid cross since the parents are homozygous, however in a dihybrid cross, the parents vary in two separate features.

3. What are the disadvantages of test cross?

The disadvantages of test cross are:

It is ineffective for quantitative traits.

It is more limited in recessive traits.

In reality, portions of the genome from non-recurrent parents are frequently present and can be associated with undesirable features.

Limited recombination may keep thousands of 'foreign' genes within the elite cultivar over extremely broad crosses.

Many backcrosses are needed to create a new cultivar, which might take years.

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2.5: The Dihybrid Test Cross

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  • Page ID 132146

  • Natasha Ramroop Singh
  • Thompson Rivers University

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While the cross of an F 1 x F 1 gives a ratio of 9:3:3:1, there is a better, easier cross to test for independent assortment: the dihybrid test cross. In a dihybrid test cross, independent assortment is seen as a ratio of 1:1:1:1, which is easier to score than the 9:3:3:1 ratio. This test cross will also be easier to use when testing for linkage.

Like in monohybrid crosses (Chapter 1), you can do test crosses with dihybrids to determine the genotype of an individual with dominant phenotypes, to see if they are heterozygous or homozygous dominant. This type of cross is set up in the same fashion; an individual with an unknown genotype in two loci is crossed to an individual that is homozygous recessive for both loci.

Two punnet squares showing different outcomes of a test cross depending on homozygous or heterozygous dihybrid organism

Take a look at the video, Two-Gene Test Cross Explained , by Nicole Lantz (2020) on YouTube, for some worked examples.

Thumbnail for the embedded element "Two-Gene Test Cross Explained"

A YouTube element has been excluded from this version of the text. You can view it online here: https://opengenetics.pressbooks.tru.ca/?p=395

Punnett squares should be done ahead of the crosses, so you know what to expect for any of the possible outcomes. Using the example from the rest of this chapter, you cross a double homozygous recessive pea plant (r/r ; y/y. green and wrinkled) to an unknown individual that has two dominant phenotypes (R/_ ; Y/_. yellow and round). There are four possible genotypes the unknown individual could be: R/R ; Y/Y or R/R ; Y/y or R/r ; Y/Y or R/r; Y/y. The Punnett squares for the first two are shown in Figure 2.5.1 . Notice on the left, you only get the dominant phenotype for both, so you know both genes in the unknown are homozygous dominant. On the right, you get only the dominant phenotype for round peas — but you get 50% yellow and 50% green peas, showing that the unknown is homozygous for round, but heterozygous for colour of the peas. Figure 2.5.2 is blank for you to fill in the other two gamete and genotype possibilities.

Blank punnet squares for learner to complete

Media Attributions

  • Figure 2.5.1 , Original by L.Canham (2017), CC BY-NC 3.0
  • Figure 2.5.2 , Original by L.Canham (2017), CC BY-NC 3.0

Canham, L. (2017). Figures: 7. Punnett square for a test cross; 8. Blank Punnett squares to fill in the other two possibilities of the test cross [digital images]. In Locke, J., Harrington, M., Canham, L. and Min Ku Kang (Eds.), Open Genetics Lectures, Fall 2017 (Chapter 17, p. 6-7). Dataverse/ BCcampus. http://solr.bccampus.ca:8001/bcc/file/7a7b00f9-fb56-4c49-81a9-cfa3ad80e6d8/1/OpenGeneticsLectures_Fall2017.pdf

Nicole Lantz. (2020, May 5). Two-gene test cross explained [Video file]. YouTube. https://youtu.be/GM0by2axiLM

Long Descriptions

  • Figure 2.5.1 Two Punnett squares: The first is a testcross between a dihybrid homozygous dominant organism (RRYY) and the tester, which is a dihybrid homozygous recessive organism (rryy). The offspring and their genotypes in this test cross are shown, with all possessing the heterozygous condition for both traits (RrYy). The second is a testcross between a dihybrid heterozygous organism (RrYy) and the tester, which is a dihybrid homozygous recessive organism (rryy). The offspring and their genotypes in this test cross are shown, with half possessing the heterozygous condition for both traits (RrYy) and the other half expressing the genotype Rryy. [Back to Figure 2.5.1 ]
  • Figure 2.5.2 A blank Punnett square can be used for practice, and to fill in the other two gamete and genotype possibilities for the question in the text directly preceding this figure. [Back to Figure 2.5.2 ]

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2.5 The Dihybrid Test Cross

While the cross of an F 1 x F 1 gives a ratio of 9:3:3:1, there is a better, easier cross to test for independent assortment: the dihybrid test cross. In a dihybrid test cross, independent assortment is seen as a ratio of 1:1:1:1, which is easier to score than the 9:3:3:1 ratio. This test cross will also be easier to use when testing for linkage.

Like in monohybrid crosses ( Chapter 1 ), you can do test crosses with dihybrids to determine the genotype of an individual with dominant phenotypes, to see if they are heterozygous or homozygous dominant. This type of cross is set up in the same fashion; an individual with an unknown genotype in two loci is crossed to an individual that is homozygous recessive for both loci.

Take a look at the video, Two-Gene Test Cross Explained , by Nicole Lantz (2020) on YouTube, for some worked examples.

Punnett squares should be done ahead of the crosses, so you know what to expect for any of the possible outcomes. Using the example from the rest of this chapter, you cross a double homozygous recessive pea plant (r/r ; y/y. green and wrinkled) to an unknown individual that has two dominant phenotypes (R/_ ; Y/_. yellow and round). There are four possible genotypes the unknown individual could be: R/R ; Y/Y or R/R ; Y/y or R/r ; Y/Y or R/r; Y/y. The Punnett squares for the first two are shown in Figure 2.5.1 . Notice on the left, you only get the dominant phenotype for both, so you know both genes in the unknown are homozygous dominant. On the right, you get only the dominant phenotype for round peas — but you get 50% yellow and 50% green peas, showing that the unknown is homozygous for round, but heterozygous for colour of the peas. Figure 2.5.2  is blank for you to fill in the other two gamete and genotype possibilities.

Media Attributions

  • Figure 2.5.1 , Original by L.Canham (2017), CC BY-NC 3.0
  • Figure 2.5.2 , Original by L.Canham (2017), CC BY-NC 3.0

Canham, L. (2017). Figures: 7. Punnett square for a test cross; 8. Blank Punnett squares to fill in the other two possibilities of the test cross [digital images]. In Locke, J., Harrington, M., Canham, L. and Min Ku Kang (Eds.),  Open Genetics Lectures, Fall 2017 (Chapter 17, p. 6-7). Dataverse/ BCcampus. http://solr.bccampus.ca:8001/bcc/file/7a7b00f9-fb56-4c49-81a9-cfa3ad80e6d8/1/OpenGeneticsLectures_Fall2017.pdf

Nicole Lantz. (2020, May 5). Two-gene test cross explained [Video file]. YouTube. https://youtu.be/GM0by2axiLM

Long Descriptions

  • Figure 2.5.1 Two Punnett squares: The first is a testcross between a dihybrid homozygous dominant organism (RRYY) and the tester, which is a dihybrid homozygous recessive organism (rryy). The offspring and their genotypes in this test cross are shown, with all possessing the heterozygous condition for both traits (RrYy). The second is a testcross between a dihybrid heterozygous organism (RrYy) and the tester, which is a dihybrid homozygous recessive organism (rryy). The offspring and their genotypes in this test cross are shown, with half possessing the heterozygous condition for both traits (RrYy) and the other half expressing the genotype Rryy. [Back to Figure 2.5.1 ]
  • Figure 2.5.2 A blank Punnett square can be used for practice, and to fill in the other two gamete and genotype possibilities for the question in the text directly preceding this figure. [Back to Figure 2.5.2 ]

Introduction to Genetics Copyright © 2023 by Natasha Ramroop Singh, Thompson Rivers University is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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United Nations Educational, Scientific and Cultural Organization

A test cross is a way to explore the genotpye of an organism. Early use of the test cross was as an experimental mating test used to determine what alleles are present in the genotype. An organism's genetic makeup is called its genotype, and it reflects all of the alleles, or forms of the gene, that are carried by the organism. Consequently, a test cross can help determine whether a dominant phenotype is homozygous or heterozygous for a specific allele.

Diploid organisms, like humans, have two alleles at each genetic locus, or position, and one allele is inherited from each parent. Different alleles do not always produce equal outward effects or phenotypes. One allele can be dominant and mask the effect of a second recessive allele in a heterozygous organism that carries two different alleles at a specific locus. Recessive alleles only express their phenotype if an organism carries two identical copies of the recessive allele, meaning it is homozygous for the recessive allele. This means that the genotype of an organism with a dominant phenotype may be either homozygous or heterozygous for the dominant allele. Therefore, it is impossible to identify the genotype of an organism with a dominant trait by visually examining its phenotype.

To identify whether an organism exhibiting a dominant trait is homozygous or heterozygous for a specific allele, a scientist can perform a test cross. The organism in question is crossed with an organism that is homozygous for the recessive trait, and the offspring of the test cross are examined. If the test cross results in any recessive offspring, then the parent organism is heterozygous for the allele in question. If the test cross results in only phenotypically dominant offspring, then the parent organism is homozygous dominant for the allele in question.

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What Is Test Cross?

Test cross: a test cross is a cross between an individual with an unknown genotype with a homozygous recessive genotype. the test cross research was initially used by gregor johann mendel. it is used to know whether the trait which is dominant is heterozygous or homozygous..

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Cross Matching : Types, Principle, Procedure and Interpretation

  • Most recipient antibodies directed against donor’s red blood cell antigens.
  • Most donor antibodies directed against recipient’s red blood cell antigens.
  • Major errors in ABO grouping , labeling and identification of donor and recipients.
  • Principle of Cross Matching

Cross matching is based on the principle of serological detection of any clinically significant irregular/unexpected antibodies in either donor or recipient’s blood. There are two types of cross matches:

  • Major Cross Match : It involves testing the donor’s red cells with recipient’s serum to determine the presence of any antibody which may cause hemolysis or agglutination of donor red cells.This is more important than minor cross match.
  • Minor Cross Match : It involves testing of donor’s plasma with recipient’s red cells to determine the presence of any antibody which may cause hemolysis or agglutination of recipient’s red cells.
  • Procedure of Cross Matching

There are different methods for cross matching, as shown in table. Among them most commonly used technique is Anti-human globulin (AHG) cross match.

  • Major Cross Match
  • Prepare donor and recipient’s blood sample: Donor’s red cells and recipient’s serum/plasma .
  • Prepare 3-5% saline cell suspension of red cells.
  • Label a test tube.
  • Add two drops of recipient’s serum and one drop of donor cell suspension.
  • Mix and incubate the tubes at 37 degree Celsius for about 60 minutes.
  • Decant the serum completely and wash the cells three times in saline.
  • Add two drops of Anti-human Globulin (AHG) and mix. Allow to stand at room temperature for 5 minutes.
  • Centrifuge at 1500 rpm for 1 minute.
  • Observe macroscopically and microscopically for agglutination.
  • If macroscopic agglutination is not observed, transfer a small amount onto a glass slide and examine for microscopic agglutination. Rouleaux is not an indication of incompatibility.

Major-Cross-Matching

  • Minor Cross Match
  • Prepare donor and recipient’s blood sample: Recipient’s red cells and donor’s serum/plasma .
  • Add two drops of donor’s serum and one drop of recipient’s cell suspension.

Minor-Cross-Matching

Results and Interpretation

Compatible donor and recipient blood should show no agglutination in both major and minor cross match. Blood which shows incompatibility in major cross match should never be transfused, because the large plasma volume of the recipient blood containing antibodies can destroy the donor’s red cells easily. The minor incompatibility is less important because the donor’s serum which contains the antibodies is diluted in the recipient’s own plasma, making the antibodies very dilute and ineffective.

  • Cross Matching
  • Interpretation of Cross Matching

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Thankyou so much sir

Why is it that after incubation of anti-human globulin for 60 minutes in a water-bath anti-human globulin reagent is added again?

1. to wash away antibody (globulin) that not grab on the red blood cells’ surface. you want to wash away the free antibody (globulin. 2. bath again with the antihuman globulin because: if there is any antibody (globulin) that have grabed on the red blood cells will show a agglutination reaction by this antihuman globulin you added. absolutely not a agglutination reaction between the free antibody (globulin) and antihuman globulin you added.

GOOD EXPLANATION AND ILLUSTRATIONS HERE. THANK YOU.

thanks good sir

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Astros pitcher Ronel Blanco suspended for 10 games after being ejected over foreign-substance check vs. A's

Blanco threw the only no-hitter of the 2024 mlb season to date on april 1.

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Houston Astros right-hander Ronel Blanco , who threw the season's only no-hitter on April 1  against the Toronto Blue Jays , has been suspended 10 games and fined an undisclosed amount for violating the league's foreign substance policy, MLB announced Wednesday. The suspension begins immediately and Blanco will not appeal, GM Dana Brown said ( via The Athletic ).

Blanco was ejected from Tuesday's win against the Oakland Athletics ( HOU 2, OAK 1 ) after umpires conferred and examined his glove prior to the start of the fourth inning. Here's the ejection:

Manager Joe Espada told reporters after the game that he saw sweat mixed with rosin in Blanco's glove ( via The Athletic ). Pitchers are not allowed to apply rosin on their non-pitching hands, however, which may have triggered the ejection.

The Astros were already planning to use a six-man rotation in the coming weeks, so they'll simply shift back to a five-man rotation during Blanco's suspension. Spencer Arrighetti , Hunter Brown , Cristian Javier , Framber Valdez , and Justin Verlander are Houston's other five starters. Blanco's next scheduled start was this Sunday, at home against the  Milwaukee Brewers .

Houston must play with a 25-man roster during Blanco's suspension. They can not replace him on the roster. Umpires conduct regular glove and uniform checks throughout the game to ensure that pitchers are not using banned substances.  The league reportedly urged umpires to be more attentive with their checks back in spring 2023 . 

Blanco, 30, owns a 2.09 ERA (182 ERA+) and a 2.14 strikeout-to-walk ratio over his first eight starts. He'd thrown three scoreless frames on Tuesday, surrendering four hits and a walk while striking out four of the 14 batters he had faced. On the whole, Blanco has been a revelation for an Astros club that, earlier this spring, was without an entire rotation's worth of veteran starting pitchers.

In the past, several other notable pitchers have been ejected and suspended under MLB's grip-enhancing substance policy. Among those: Texas Rangers ace Max Scherzer , Pittsburgh Pirates minor-league right-hander Domingo Germán, and San Diego Padres closer Robert Suárez. In all, six big-league pitchers have been punished for violating the policy.

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They may not have won much on the field, but they sure won the back page.

The 1990 Yankees, the franchise’s worst team in the past 100 years but a roster that sure kept things interesting, took centerstage Thursday with the release of the three-part docuseries “Bronx Zoo ‘90: Crime, Chaos and Baseball” on NBC’s Peacock, in conjunction with The Post.

Directed by veteran filmmaker DJ Caruso, the docuseries is based off New York Post sports columnist and then-Yankees beat writer Joel Sherman’s eight-part series in June 2020 that relived the true nadir before the birth of the dynasty in 1996 as chaos engulfed a 67-95 team.

The three-part docuseries features 60-minute episodes.

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The 1990 Yankees hold the dubious distinction of owning the fourth-worst winning percentage in franchise history and the second-worst winning percentage since the franchise following the Highlanders days.

It’s not often that such a team would be memorable, but this Yankees group kept things quite interesting.

“I covered the team when free agents used the Yankees to bid themselves up with no plan whatsoever to sign with them,” Sherman told Decider. “Who wanted to play there when the owner might target you, like he did to Dave Winfield and others?… It was it was the worst show on on Earth, and nobody wanted to be part of it.”

The docuseries covers all the unbelievable moments, including manager Bucky Dent’s stunning firing in Boston — home of his greatest moment as a player.

Bucky Dent during his managerial days.

“When Bucky got fired, he’s willing to talk and he said, ‘I just can’t believe he fired me in Boston,'” Kay says in the series . “Because it was the ultimate insult. This was Bucky-bleeping-Dent from 1978, the MVP.

“He got fired in Boston, which was, I mean, probably the unkindest kick in the cubes you could have.”

Other memorable moments from that season include George Steinbrenner being suspended after paying gambler Howie Spira — who is interviewed — for dirt on Winfield, a star outfielder.

There’s Mel Hall’s penchant for bringing two cougars into the clubhouse, along with his scandalous relationship with 15-year-old Chastity Easterly.

In 2009, Hall landed in jail on a 45-year sentence for raping a 12-year-old girl, and both he and Easterly –the former from prison, the latter for the first time — are interviewed in the series.

“It was really eye-opening for me to kind of go there and interview Mel in jail,” Caruso told NBC. “It wasn’t what I expected. I expected someone to kind of maybe have a little bit more remorse, but he didn’t really seem to have that. It was interesting. He sort of was still portraying himself as the victim in his own story.”

Buck Showalter (l) and Georgie Steinbrenner (r).

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And who could forget Pascual Perez, who signed a three-year, $5.7 million contract in the offseason, no-showing the start of camp and being summoned for a workout before lasting just three starts.

There’s a reason Showalter is quoted in the docuseries saying: “I’ve been trying to forget that team.”

“Think about how arms are trying to be protected these days, especially guys you spend a lot of money on,” Sherman said. “Here they were, they rolled this guy out to throw a bullpen at night to punish him in front of the media. And I’m picking out something crazy from a year of cougar cubs and the owner being suspended and a guy throwing a no-hitter and losing a game.”

A headline in the New York Post after Andy Hawkins' tossed a no-hitter in a losing effort.

Through all the craziness, that season did help pave the way for the Yankees’ run of four titles in five years, spearheaded by general manager Gene Michael .

“It just became to me, thematically, a look under the hood or look behind the curtain of this dynasty that emerged in the ’90s,” Caruso told NBC. “How could a team that was so bad on the field and off the field in three years turn this thing around and become the greatest franchise in the history of all sports?”

He added: “Like all good stories, whenever you go through these periods of darkness, it’s how you come out of it on the other end. So, you’re going to see that this franchise rose from this dark period and came out with great light. But within the darkness, there are so many great interpersonal stories. Some are tragic, some are mind-boggling.”

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Consumers looking for a three-row crossover SUV for the family will struggle to top the 2024 Kia EV9.

The EV9 electric SUV took top honors this year and was named The Car Connection Best Car To Buy 2024 and Best Family Car To Buy 2024 . Months later, I have no regrets about the way we voted. This thing is aces.

With impressive efficiency, a comfortable and well-packaged interior, and easy-to-use controls the EV9 wins over adults and kids. Its TCC Rating of 8.6 out of 10 is one of the highest of the 260 or so vehicles on The Car Connection, but it misses out on a point here or there due to over- and under-thinking a few things, its smartphone companion app isn’t the best, and it gets expensive. 

Here are the pros and cons of living with the Kia EV9 for a week.

2024 Kia EV9

2024 Kia EV9

2024 Kia EV9

Pro: EV9 features great packaging for the family

Like the Kia Telluride but better, the EV9 fits the family with ease. At 5-foot-10 I could comfortably sit in all three rows with the seats adjusted for a comfortable seating position. My kids never complained once about space, and they complain about everything. The front seat headrests have pillowy mesh design with a metal surround like something found on a Herman Miller Aeron desk chair. This thing’s road trip worthy, though I had to explain to my kids that my tester’s power reclining second-row seats shouldn’t be reclined while in motion for safety reasons. That did induce some whining. 

2024 Kia EV9

Con: EV9 over- and under-thinks certain family features

My loaded GT-Line AWD tester featured power-operated second-row seats that could slide, recline, and even had foot rests. Tapping the button on the top of the seat back to slide and fold the second row for third-row access was a painfully slow experience. What parent has this kind of time? It’s similar to what’s found in the BMW X7, and it’s excruciatingly slow there as well, but this isn’t a luxury vehicle, despite the nearly $80,000 price tag. The second row needs to be able to slide and tilt quicker, whether electronically or manually.

Families getting into the EV9 will note the rear door handles don’t feature a touch-to-unlock sensor like most vehicles in this price class. Even the Nissan Pathfinder and Jeep Grand Cherokee L feature this, though notably it’s missing on the Kia Telluride and Hyundai Palisade as well.

2024 Kia EV9

Pro: EV9 surprises with efficiency

Despite its blocky exterior design the EV9 is actually efficient. Base EV9s feature a 215-hp single rear motor paired with a 76.1-kwh battery pack for 230 miles of EPA-rated range. I had the larger 99.8-kwh battery with two electric motors rated at 379 hp and all-wheel drive delivering an EPA-rated range of 270 miles.  Most of the week I actually averaged between 3.0 and 3.1 mi/kwh in mixed suburban driving, which is efficient given the EV9’s size, weight, and design. Eventually some higher highway speeds and a little more aggressive driving dropped my efficiency down to 2.8 mi/kwh over the course of 222 miles. 

2024 Kia EV9

Con: EV9 gets to pricey

The EV9 isn’t a luxury vehicle, doesn’t get bought or serviced at a luxury dealership, doesn’t wear a luxury badge, or feature luxury materials in its cabin. Base models cost $56,395 including a $1,495 destination fee, which is the cost of a nearly loaded Telluride. It’s also about $7,000 less than a three-row Tesla Model X and $18,000 less than the Rivian R1S. The longest range EV9 with 304 miles costs $60,695, but it’ll cost $65,395 or more to get a dual-motor model with all-wheel drive. My loaded Long Range AWD GT-Line model cost a luxury-like $78,430 thanks to the $2,000 second-row relaxation package, optional $695 blue paint (absolutely worth it), $225 carpeted floor mats, and $115 cargo area carpeted mat. That’s a lot of coin for the Kia dealership experience and recycled interior materials, which are nice, but not at the level of a BMW or even a loaded Grand Cherokee L Summit.

2024 Kia EV9

Pro: EV9 features toggles and buttons

Key functions in the EV9 aren’t buried in, or even require, the three screens on the dashboard. There’s a roller knob for the audio volume; toggles for the climate control temp, fan speed, and  air direction; real buttons, rollers, and rockers on the steering wheel; and hard buttons for the charge port door, frunk, tailgate, and display brightness. There’s even buttons on the doors to control the heated and ventilated front and rear seats, which my kids made sure I was aware of.

2024 Kia EV9

Con: Kia’s smartphone app feels behind

Kia’s impressive family car of the future feels behind when it comes to the smartphone user experience. The Apple iOS app, like Hyundai’s, works, but it’s a full league behind apps by Tesla, Rivian, and Lucid. The app at times was slow, required constant loading, and hilariously and frustratingly required confirmation for functions like locking the car, unlocking the car, or starting the climate control system. The confirmation would come with a delay and I was left waiting to wonder if the request was executed. 

It’s clear the EV9 is the three-row electric crossover SUV for families ready to move on from gas. It’s the best value in its electric three-row segment (for now) and is a great family car.

_______________________________________

2024 Kia EV9 Long Range AWD GT-Line

Base price : $56,395, including a $1,495 destination fee

Price as tested : $78,430

Drivetrain : 379 hp combined, dual-motor AWD

EPA range : 270 miles

The hits : Efficient, well packaged, real buttons, sharp design

The misses : Gets expensive, tolerable iOS app, slow-moving second row

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Kia EV9: The Car Connection Best Car To Buy 2024

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assignment on test cross

IMAGES

  1. Test Cross

    assignment on test cross

  2. Test Cross (Single, Two, Triple Gene)- Definition, Examples, Uses

    assignment on test cross

  3. Test Cross- Definition and Examples

    assignment on test cross

  4. Testcross

    assignment on test cross

  5. What is Test cross? Made easy with example

    assignment on test cross

  6. Explain back cross and test cross with example

    assignment on test cross

VIDEO

  1. Test Cross

  2. Create Assignment Test Premiere

  3. Fact/Value/Policy Assignment- Opposition Cross Examination

  4. Mid Test Cross Culture Understanding

  5. Genetics: 3-point Test Cross Practice Question (made rly quickly)

  6. Day 8

COMMENTS

  1. Test Cross

    The typical example of the test cross is the origin experiment Mendel conducted himself, to determine the genotype of a yellow pea. As seen in the image below, the alleles Y and y are used for the yellow and green versions of the allele, respectively. The yellow allele, Y, is dominant over the y allele. Therefore, in an organism with the ...

  2. Test Cross (Single, Two, Triple Gene)- Definition, Examples, Uses

    Also called 'single gene test cross', is a type of testcross where only one type of gene or phenotypic character is studied. Among different characters of test individuals, only one of the dominant characters is considered. In a monohybrid test cross, a 1:1 phenotypic ratio is obtained if the test individual is heterozygous.

  3. PDF Test Cross Worksheet (A

    TEST-CROSS RULES: A. Always cross the organism with the unknown genotype with an organism that is Homozygous Recessive (two lowercase alleles) B. If possible, examine large numbers of offspring to ensure accuracy in determining the unknown genotype. Traits. 1. In fruit flies, red eyes are dominant over brown eyes.

  4. PDF BIO102 Test Cross

    Unit 1.5.2 Assignment Test Cross Genetic testcrosses are used when the genotype of an organism is unknown. Test crosses have been around since the earliest genetics work, including Thomas Hunt Morgan's work with fruit flies and sex-linked genes (Miko 2008). Test crosses are simple. For example, if you have an individual plant that is tall

  5. Assignment 2 Testcross-2

    Assignment 2: T estcr oss. A testcross is a valuable way to use a genetic cross to determine the genotype of an organism that shows. a dominant phenotyp e but unknown genotype. F or inst ance, using Mendel's peas, a pea plan t with.

  6. Test Cross- Definition and Examples

    Test Cross. A test cross is a genetic technique discovered by Gregor Mendel that entails mating an individual with all phenotypically recessive individuals, to ascertain the zygosity of the former by evaluating the proportions of offspring phenotypes. Zygosity can be homozygous or heterozygous. Heterozygous individuals have one dominant and one ...

  7. Test Crosses

    Now, say you carry out the test cross and obtain 400 progeny. You sort these progeny by phenotype and discover that you have 200 flies with a yellow body and red eyes, as well as 200 progeny with ...

  8. Test cross

    These test cross experiments became hallmarks in the discovery of sex-linked traits. Test cross types. A test cross involves crossing an individual organism with a dominant genotype or phenotype with another organism exhibiting a recessive genotype or phenotype. To better grasp the concept of test crossing, let's explore various types of ...

  9. 2.5: The Dihybrid Test Cross

    While the cross of an F 1 x F 1 gives a ratio of 9:3:3:1, there is a better, easier cross to test for independent assortment: the dihybrid test cross. In a dihybrid test cross, independent assortment is seen as a ratio of 1:1:1:1, which is easier to score than the 9:3:3:1 ratio. This test cross will also be easier to use when testing for linkage.

  10. 2.5 The Dihybrid Test Cross

    2.5 The Dihybrid Test Cross. While the cross of an F 1 x F 1 gives a ratio of 9:3:3:1, there is a better, easier cross to test for independent assortment: the dihybrid test cross. In a dihybrid test cross, independent assortment is seen as a ratio of 1:1:1:1, which is easier to score than the 9:3:3:1 ratio. This test cross will also be easier ...

  11. The test cross

    The test cross is carried out in order to determine whether a dominant phenotype is homozygous or heterozygous. This is always crossed with the homozygous recessive. If the dominant phenotype is heterozygous, we would expect 50% of the offspring to show the recessive phenotype.

  12. test cross

    test cross. A test cross is a way to explore the genotpye of an organism. Early use of the test cross was as an experimental mating test used to determine what alleles are present in the genotype ...

  13. CROSS PREDICTIONS Flashcards

    Unit 5 - Assignment 4 Learn with flashcards, games, and more — for free. ... The results of a test cross of a tall tomato plant was 100% tall. Fill in the punnett square if all the recessive alleles (genes) came from pollen during the cross test. ⁣ ⁣ ⁣⁣ 〡T⠀〡T ...

  14. Three Point Test Cross: Multiple Point Gene Mapping

    First we will explain the steps and then we will show how to use them to map the three genes. Step 1: Identify the parental gametes. These are CsW and cSw, that combination which came from the parent generation and the combination made by the trihybrid at the highest frequency. Step 2: Classify the recombinants.

  15. Testcross (Backcross; concepts of parental, F1, and F2 generations)

    A test cross is a way to determine whether an organism that expressed a dominant trait was homozygous or heterozygous; backcross is the mating between parent and offspring to preserve the parental genotype; P represents parent, F1 (filial 1) represents the children of the parent and F2 represents the children of the F1.. In genetics, dominant alleles are assigned capital letters (e.g., AA ...

  16. Monohybrid and Test Cross Practice Problems WS 1A

    Monohybrid & Test Cross Practice WS 1A Name: _____ Honors Biology - Introduction to Genetics Objective: In this activity you will practice with one trait crosses (monohybrid) as well as test crosses. Use the following diagram to answer questions 1-5. 1. Describe the following monohybrid cross of plants.

  17. Chapter 11 Genetics 1 Assignment Flashcards

    A plant grown from a [round, yellow] seed is crossed with a plant grown from a [wrinkled, yellow] seed. This cross produces four progeny types in the F1: [round, yellow], [wrinkled, yellow], [round, green], and [wrinkled, green]. Use this information to deduce the genotypes of the parent plants. Indicate the genotypes by dragging the correct ...

  18. Dihybrid cross and the law of independent assortment

    Flag. Patrick Kang. 2 months ago. Dihybrid cross is just the name given to crossing of two identically heterozygous individuals, and it happens that it results in a 9:3:3:1 ratio. You can cross any two individuals by using a Punnet square, but they would not have the same 9:3:3:1 ratio. ( 1 vote) Upvote.

  19. Lab: Mouse Genetics (One Trait) Assignment: Reflect on the ...

    Study with Quizlet and memorize flashcards containing terms like Throughout the reflection, make sure you have a copy of the Student Guide and your data table. In your experiment, you tested this hypothesis: Hypothesis: If either parent mouse passes a dominant allele, then the offspring will have black fur. You confirmed the above in steps 1 through 3, in which you changed the genotypes of the ...

  20. Testcrosses

    Tetrahymena Thermophila. Eduardo Orias, in Methods in Cell Biology, 2012. G Test-Crossing Progeny Cell Lines. Because of dominance, phenotypic tests of young cultures are generally insufficient to directly determine their genotype, and testcrosses are required after the cells have become sexually mature. A general, simple, and convenient way to testcross a cell line is to cross it either to a ...

  21. What Is Test Cross?

    Test Cross: A test cross is a cross between an individual with an unknown genotype with a homozygous recessive genotype. The test cross research was initially used by Gregor Johann Mendel. It is used to know whether the trait which is dominant is heterozygous or homozygous. Suggest Corrections. 82. Similar questions.

  22. Cross Matching : Types, Principle, Procedure and Interpretation

    Major Cross Match. Prepare donor and recipient's blood sample: Donor's red cells and recipient's serum/plasma. Prepare 3-5% saline cell suspension of red cells. Label a test tube. Add two drops of recipient's serum and one drop of donor cell suspension. Mix and incubate the tubes at 37 degree Celsius for about 60 minutes.

  23. Critical days of Trump trial will test whether he can exercise ...

    The Assignment with Audie Cornish ... Critical days of Trump trial will test whether he can exercise discipline and restraint ... He can therefore be expected to face an even more withering cross ...

  24. Astros pitcher Ronel Blanco ejected after foreign-substance check vs. A

    Astros pitcher Ronel Blanco ejected after foreign-substance check vs. A's, could face 10-game suspension Blanco threw the only no-hitter of the 2024 MLB season to date on April 1

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    The three-part docuseries features 60-minute episodes. Peacock. This inside look at the Bronx Zoo includes first-hand accounts of that season from Yankees legends such as Don Mattingly and Bernie ...

  26. Test drive: 2024 Volkswagen Atlas Cross Sport takes two steps forward

    Every Atlas Cross Sport (and Atlas) are now powered by a new 2.0-liter turbo-4 rated at 296 hp and 273 lb-ft of torque. Before anyone breaks out the slide rules, that's down 7 hp and up 6 lb-ft ...

  27. Lab: Mouse Genetics (One Trait) Assignment: Reflect on the Lab

    8 mice with black fur, 1 mouse with white fur. 5 mice with black fur, 6 mice with white fur. 6 mice with black fur, 2 mice with white fur. There are genes that govern the eye color of mice, too. Suppose that a true-breeding mouse with red eyes is crossed with a true-breeding mouse with black eyes. All of the offspring mice have black eyes.

  28. Test drive: 2024 Kia EV9 remains a winner

    Test drive: 2024 Volkswagen Atlas Cross Sport takes two steps forward, one back. Test drive: 2024 Kia EV9 remains a winner. Test drive: 2024 Chevrolet Trailblazer aims for all-weather value.

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    Performance is perfectly adequate. The 7.4-second -60-mph time undercuts that of both the new gas Crosstrek and the outgoing PHEV version by more than a half second, and of the Niro PHEV by ...

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