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Molecular Biosciences Theses and Dissertations

Theses/dissertations from 2023 2023.

Exploring strain variation and bacteriophage predation in the gut microbiome of Ciona robusta , Celine Grace F. Atkinson

Distinct Nrf2 Signaling Thresholds Mediate Lung Tumor Initiation and Progression , Janine M. DeBlasi

Thermodynamic frustration of TAD2 and PRR contribute to autoinhibition of p53 , Emily Gregory

Utilization of Detonation Nanodiamonds: Nanocarrier for Gene Therapy in Non-Small Cell Lung Cancer , Allan E. Gutierrez

Role of HLA-DRB1 Fucosylation in Anti-Melanoma Immunity , Daniel K. Lester

Targeting BET Proteins Downregulates miR-33a To Promote Synergy with PIM Inhibitors in CMML , Christopher T. Letson

Regulated Intramembrane Proteolysis by M82 Peptidases: The Role of PrsS in the Staphylococcus aureus Stress Response , Baylie M. Schott

Histone Deacetylase 8 is a Novel Therapeutic Target for Mantle Cell Lymphoma and Preserves Natural Killer Cell Cytotoxic Function , January M. Watters

Theses/Dissertations from 2022 2022

Regulation of the Heat Shock Response via Lysine Acetyltransferase CBP-1 and in Neurodegenerative Disease in Caenorhabditis elegans , Lindsey N. Barrett

Determining the Role of Dendritic Cells During Response to Treatment with Paclitaxel/Anti-TIM-3 , Alycia Gardner

Cell-free DNA Methylation Signatures in Cancer Detection and Classification , Jinyong Huang

The Role Of Eicosanoid Metabolism in Mammalian Wound Healing and Inflammation , Kenneth D. Maus

A Holistic Investigation of Acidosis in Breast Cancer , Bryce Ordway

Characterizing the Impact of Postharvest Temperature Stress on Polyphenol Profiles of Red and White-Fruited Strawberry Cultivars , Alyssa N. Smith

Theses/Dissertations from 2021 2021

Multifaceted Approach to Understanding Acinetobacter baumannii Biofilm Formation and Drug Resistance , Jessie L. Allen

Cellular And Molecular Alterations Associated with Ovarian and Renal Cancer Pathophysiology , Ravneet Kaur Chhabra

Ecology and diversity of boletes of the southeastern United States , Arian Farid

CircREV1 Expression in Triple-Negative Breast Cancer , Meagan P. Horton

Microbial Dark Matter: Culturing the Uncultured in Search of Novel Chemotaxonomy , Sarah J. Kennedy

The Multifaceted Role of CCAR-1 in the Alternative Splicing and Germline Regulation in Caenorhabditis elegans , Doreen Ikhuva Lugano

Unraveling the Role of Novel G5 Peptidase Family Proteins in Virulence and Cell Envelope Biogenesis of Staphylococcus aureus , Stephanie M. Marroquin

Cytoplasmic Polyadenylation Element Binding Protein 2 Alternative Splicing Regulates HIF1α During Chronic Hypoxia , Emily M. Mayo

Transcriptomic and Functional Investigation of Bacterial Biofilm Formation , Brooke R. Nemec

A Functional Characterization of the Omega (ω) subunit of RNA Polymerase in Staphylococcus aureus , Shrushti B. Patil

The Role Of Cpeb2 Alternative Splicing In TNBC Metastasis , Shaun C. Stevens

Screening Next-generation Fluorine-19 Probe and Preparation of Yeast-derived G Proteins for GPCR Conformation and Dynamics Study , Wenjie Zhao

Theses/Dissertations from 2020 2020

Understanding the Role of Cereblon in Hematopoiesis Through Structural and Functional Analyses , Afua Adutwumwa Akuffo

To Mid-cell and Beyond: Characterizing the Roles of GpsB and YpsA in Cell Division Regulation in Gram-positive Bacteria , Robert S. Brzozowski

Spatiotemporal Changes of Microbial Community Assemblages and Functions in the Subsurface , Madison C. Davis

New Mechanisms That Regulate DNA Double-Strand Break-Induced Gene Silencing and Genome Integrity , Dante Francis DeAscanis

Regulation of the Heat Shock Response and HSF-1 Nuclear Stress Bodies in C. elegans , Andrew Deonarine

New Mechanisms that Control FACT Histone Chaperone and Transcription-mediated Genome Stability , Angelo Vincenzo de Vivo Diaz

Targeting the ESKAPE Pathogens by Botanical and Microbial Approaches , Emily Dilandro

Succession in native groundwater microbial communities in response to effluent wastewater , Chelsea M. Dinon

Role of ceramide-1 phosphate in regulation of sphingolipid and eicosanoid metabolism in lung epithelial cells , Brittany A. Dudley

Allosteric Control of Proteins: New Methods and Mechanisms , Nalvi Duro

Microbial Community Structures in Three Bahamian Blue Holes , Meghan J. Gordon

A Novel Intramolecular Interaction in P53 , Fan He

The Impact of Myeloid-Mediated Co-Stimulation and Immunosuppression on the Anti-Tumor Efficacy of Adoptive T cell Therapy , Pasquale Patrick Innamarato

Investigating Mechanisms of Immune Suppression Secondary to an Inflammatory Microenvironment , Wendy Michelle Kandell

Posttranslational Modification and Protein Disorder Regulate Protein-Protein Interactions and DNA Binding Specificity of p53 , Robin Levy

Mechanistic and Translational Studies on Skeletal Malignancies , Jeremy McGuire

Novel Long Non-Coding RNA CDLINC Promotes NSCLC Progression , Christina J. Moss

Genome Maintenance Roles of Polycomb Transcriptional Repressors BMI1 and RNF2 , Anthony Richard Sanchez IV

The Ecology and Conservation of an Urban Karst Subterranean Estuary , Robert J. Scharping

Biological and Proteomic Characterization of Cornus officinalis on Human 1.1B4 Pancreatic β Cells: Exploring Use for T1D Interventional Application , Arielle E. Tawfik

Evaluation of Aging and Genetic Mutation Variants on Tauopathy , Amber M. Tetlow

Theses/Dissertations from 2019 2019

Investigating the Proteinaceous Regulome of the Acinetobacter baumannii , Leila G. Casella

Functional Characterization of the Ovarian Tumor Domain Deubiquitinating Enzyme 6B , Jasmin M. D'Andrea

Integrated Molecular Characterization of Lung Adenocarcinoma with Implications for Immunotherapy , Nicholas T. Gimbrone

The Role of Secreted Proteases in Regulating Disease Progression in Staphylococcus aureus , Brittney D. Gimza

Advanced Proteomic and Epigenetic Characterization of Ethanol-Induced Microglial Activation , Jennifer Guergues Guergues

Understanding immunometabolic and suppressive factors that impact cancer development , Rebecca Swearingen Hesterberg

Biochemical and Proteomic Approaches to Determine the Impact Level of Each Step of the Supply Chain on Tomato Fruit Quality , Robert T. Madden

Enhancing Immunotherapeutic Interventions for Treatment of Chronic Lymphocytic Leukemia , Kamira K. Maharaj

Characterization of the Autophagic-Iron Axis in the Pathophysiology of Endometriosis and Epithelial Ovarian Cancers , Stephanie Rockfield

Understanding the Influence of the Cancer Microenvironment on Metabolism and Metastasis , Shonagh Russell

Modeling of Interaction of Ions with Ether- and Ester-linked Phospholipids , Matthew W. Saunders

Novel Insights into the Multifaceted Roles of BLM in the Maintenance of Genome Stability , Vivek M. Shastri

Conserved glycine residues control transient helicity and disorder in the cold regulated protein, Cor15a , Oluwakemi Sowemimo

A Novel Cytokine Response Modulatory Function of MEK Inhibitors Mediates Therapeutic Efficacy , Mengyu Xie

Novel Strategies on Characterizing Biologically Specific Protein-protein Interaction Networks , Bi Zhao

Theses/Dissertations from 2018 2018

Characterization of the Transcriptional Elongation Factor ELL3 in B cells and Its Role in B-cell Lymphoma Proliferation and Survival , Lou-Ella M.m. Alexander

Identification of Regulatory miRNAs Associated with Ethanol-Induced Microglial Activation Using Integrated Proteomic and Transcriptomic Approaches , Brandi Jo Cook

Molecular Phylogenetics of Floridian Boletes , Arian Farid

MYC Distant Enhancers Underlie Ovarian Cancer Susceptibility at the 8q24.21 Locus , Anxhela Gjyshi Gustafson

Quantitative Proteomics to Support Translational Cancer Research , Melissa Hoffman

A Systems Chemical Biology Approach for Dissecting Differential Molecular Mechanisms of Action of Clinical Kinase Inhibitors in Lung Cancer , Natalia Junqueira Sumi

Investigating the Roles of Fucosylation and Calcium Signaling in Melanoma Invasion , Tyler S. Keeley

Synthesis, Oxidation, and Distribution of Polyphenols in Strawberry Fruit During Cold Storage , Katrina E. Kelly

Investigation of Alcohol-Induced Changes in Hepatic Histone Modifications Using Mass Spectrometry Based Proteomics , Crystina Leah Kriss

Off-Target Based Drug Repurposing Using Systems Pharmacology , Brent M. Kuenzi

Investigation of Anemarrhena asphodeloides and its Constituent Timosaponin-AIII as Novel, Naturally Derived Adjunctive Therapeutics for the Treatment of Advanced Pancreatic Cancer , Catherine B. MarElia

The Role of Phosphohistidine Phosphatase 1 in Ethanol-induced Liver Injury , Daniel Richard Martin

Theses/Dissertations from 2017 2017

Changing the Pathobiological Paradigm in Myelodysplastic Syndromes: The NLRP3 Inflammasome Drives the MDS Phenotype , Ashley Basiorka

Modeling of Dynamic Allostery in Proteins Enabled by Machine Learning , Mohsen Botlani-Esfahani

Uncovering Transcriptional Activators and Targets of HSF-1 in Caenorhabditis elegans , Jessica Brunquell

The Role of Sgs1 and Exo1 in the Maintenance of Genome Stability. , Lillian Campos-Doerfler

Mechanisms of IKBKE Activation in Cancer , Sridevi Challa

Discovering Antibacterial and Anti-Resistance Agents Targeting Multi-Drug Resistant ESKAPE Pathogens , Renee Fleeman

Functional Roles of Matrix Metalloproteinases in Bone Metastatic Prostate Cancer , Jeremy S. Frieling

Disorder Levels of c-Myb Transactivation Domain Regulate its Binding Affinity to the KIX Domain of CREB Binding Protein , Anusha Poosapati

Role of Heat Shock Transcription Factor 1 in Ovarian Cancer Epithelial-Mesenchymal Transition and Drug Sensitivity , Chase David Powell

Cell Division Regulation in Staphylococcus aureus , Catherine M. Spanoudis

A Novel Approach to the Discovery of Natural Products From Actinobacteria , Rahmy Tawfik

Non-classical regulators in Staphylococcus aureus , Andy Weiss

Theses/Dissertations from 2016 2016

In Vitro and In Vivo Antioxidant Capacity of Synthetic and Natural Polyphenolic Compounds Identified from Strawberry and Fruit Juices , Marvin Abountiolas

Quantitative Proteomic Investigation of Disease Models of Type 2 Diabetes , Mark Gabriel Athanason

CMG Helicase Assembly and Activation: Regulation by c-Myc through Chromatin Decondensation and Novel Therapeutic Avenues for Cancer Treatment , Victoria Bryant

Computational Modeling of Allosteric Stimulation of Nipah Virus Host Binding Protein , Priyanka Dutta

Cell Cycle Arrest by TGFß1 is Dependent on the Inhibition of CMG Helicase Assembly and Activation , Brook Samuel Nepon-Sixt

Gene Expression Profiling and the Role of HSF1 in Ovarian Cancer in 3D Spheroid Models , Trillitye Paullin

VDR-RIPK1 Interaction and its Implications in Cell Death and Cancer Intervention , Waise Quarni

Regulation of nAChRs and Stemness by Nicotine and E-cigarettes in NSCLC , Courtney Schaal

Targeting Histone Deacetylases in Melanoma and T-cells to Improve Cancer Immunotherapy , Andressa Sodre De Castro Laino

Nonreplicative DNA Helicases Involved in Maintaining Genome Stability , Salahuddin Syed

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Home > CNS > BIOCHEM > MCB > MCB_DISS

Molecular and Cellular Biology Dissertations Collection

Dissertations from 2024 2024.

POSTSYNAPTIC CHANGES IN INHIBITORY SIGNALING ARE NOT NECESSARY FOR EXPERIENCE-DEPENDENT MAINTENANCE OF THE VISUAL PATHWAY , Parag S. Juvale and Parag S. Juvale, Molecular and Cellular Biology

INVESTIGATING THE ESCAPE MECHANISM OF SRE BEARING MRNA TRANSCRIPTS DURING VIRAL HOST SHUTOFF , Daniel MacVeigh-Fierro, Molecular and Cellular Biology

Visual Experience is Necessary for Maintenance but not Development of Lateral Inhibition in Superior Colliculus , Christine M. Otfinoski, Molecular and Cellular Biology

Dissertations from 2023 2023

Establishing Personalized Medicine in Fabry Disease Through Functional Analysis of Disease Mutants , Efecan Aral, Molecular and Cellular Biology

PROTEIN TRAFFICKING IN THE ENDOPLASMIC RETICULUM MEDIATED BY A TPR-CONTAINING ADAPTER PROTEIN , Nathan P. Canniff, Molecular and Cellular Biology

THE GENOMIC DYNAMICS OF FERMENTED FOOD MICROBES , Katherine Chacón-Vargas, Molecular and Cellular Biology

Characterizing the roles of the variable linker and hub domains in CaMKII activation , Noelle Dziedzic, Molecular and Cellular Biology

Intracellular Delivery of siRNA Therapeutics through Polymer Nanoassemblies , Taewon Jeon, Molecular and Cellular Biology

Investigating Signaling Mechanisms of Neuronal Maturation and Synapse Function Using Human Induced Neuronal Cells , Danny B. McSweeney, Molecular and Cellular Biology

Investigating Conformational Isomers of Protein Oligomers using Native Mass Spectrometry , Stacey G. Nash, Molecular and Cellular Biology

TRANSCRIPTIONAL REGULATION OF BONE FORMATION, MECHANOSENSING, AND EVOLUTION , Emily R. Tetrault, Molecular and Cellular Biology

Molecular analysis of phase separation on the plant cell surface , Robert Yvon, Molecular and Cellular Biology

Dissertations from 2022 2022

CHARACTERIZING THE MULTIFACETED ROLES OF THE PROTEASOMAL DEUBIQUITINASE UCH37 IN PROTEOSTASIS , Heather A. Bisbee, Molecular and Cellular Biology

Synthesis and targeting of the bacterial cell wall , Alam García-Heredia, Molecular and Cellular Biology

Genetic and Environmental Regulation of Plant Growth , Kirk J-M MacKinnon, Molecular and Cellular Biology

Principles of AAA+ Proteases , Samar Mahmoud, Molecular and Cellular Biology

CHEMICAL BIOLOGY APPROACHES FOR TRACKING AND MANIPULATION OF MACROPHAGE PHENOTYPES , Javier A. Mas Rosario, Molecular and Cellular Biology

Exposure to Endocrine Disrupting Chemicals and the Effects on Inflammation and Mammary Tumor Progression , Stephanie Morin, Molecular and Cellular Biology

STRUCTURAL STUDIES OF CaMKII ACTIVATION AND OLIGOMERIZATION , Can Özden, Molecular and Cellular Biology

Human Saposin B Ligand Binding and Presentation to α-Galactosidase A , Thomas K. Sawyer, Molecular and Cellular Biology

EXTRACELLULAR MATRIX STIFFNESS AS A CUE TO SHAPE PHENOTYPIC EVOLUTION OF TRIPLE NEGATIVE BREAST CANCER , Ning-Hsuan Tseng, Molecular and Cellular Biology

Dissertations from 2021 2021

Substrate selection in endoplasmic reticulum protein quality control , Ben Adams, Molecular and Cellular Biology

MECHANISMS OF MITOTIC CHECKPOINT SILENCING BY THE DISORDERED KINETOCHORE PROTEIN SPC105 , Margaux Audett, Molecular and Cellular Biology

DISCOVERING MECHANISMS DRIVING ADAPTIVE EVOLUTION IN THE CROSS-KINGDOM FUNGAL PATHOGEN FUSARIUM OXYSPORUM , Dilay Hazal Ayhan, Molecular and Cellular Biology

BIOCHEMICAL STUDIES OF FABRY DISEASE AND FUCOSIDOSIS , Cameron Butova, Molecular and Cellular Biology

PHARMACOLOGICAL CHAPERONING OF HUMAN LYSOSOMAL NEURAMINIDASE 1 , Di Chu, Molecular and Cellular Biology

RAPID CELL PHENOTYPING USING ARRAY-BASED SENSORS: APPLICATIONS IN CANCER STEM CELL THERAPY AND HIGH-CONTENT SCREENING , Yingying Geng, Molecular and Cellular Biology

Mechanistic Insights into Diverse Protease Adaptor Functions , NATHAN J. KUHLMANN, Molecular and Cellular Biology

It Takes a Village to Build a Brain: Defining the Heterogenous Glial and Neural Crest Contributions to Zebrafish Forebrain Development and Neurogenesis , Jake Schnabl, Molecular and Cellular Biology

Understanding how CaMKII holoenzyme dynamics facilities activation-triggered subunit exchange , Ana P. Torres-Ocampo, Molecular and Cellular Biology

APPLYING GENOME-WIDE ASSOCIATION IN THE FUNGAL PATHOGEN ASPERGILLUS FUMIGATUS TO IDENTIFY THE GENETIC CONTRIBUTORS OF ANTIFUNGAL SENSITIVITY AND THERMOTOLERANCE , Shu Zhao, Molecular and Cellular Biology

Dissertations from 2020 2020

Exploring Signatures of Host-Microbial Coevolution Between Colonic Bifidobacterium Species and Host Dietary Carbohydrates , Korin Albert, Molecular and Cellular Biology

Defining the let-7 microRNA-mediated molecular mechanisms regulating T cell differentiation , Constance C. Angelou, Molecular and Cellular Biology

INTER-INDIVIDUAL VARIATION IN RESPONSE TO ESTROGEN AND IMPLICATIONS FOR BREAST CANCER RISK , Amye L. Black, Molecular and Cellular Biology

TPR-CONTAINING PROTEINS CONTROL PROTEIN ORGANIZATION AND HOMEOSTASIS FOR THE ENDOPLASMIC RETICULUM , Jill Bradley-Graham, Molecular and Cellular Biology

Dissecting regulatory mechanisms of quorum sensing pathways in Bacillus subtilis , Patrick Hill, Molecular and Cellular Biology

Multivesicular Release in Hair Cell Ribbon Synapses in the Bullfrog Amphibian Papilla , Fuu-Jiun Hwang, Molecular and Cellular Biology

ROLES OF RECN DNA REPAIR PROTEIN AND PRIA DNA HELICASE IN MAINTAINING GENOMIC INTEGRITY OF ESCHERICHIA COLI K-12 , Anastasiia N. Klimova, Molecular and Cellular Biology

Hedgehog Signaling (Hh/Gli) Regulates Neurogenesis in the Zebrafish Hypothalamus , Ira Male, Molecular and Cellular Biology

A Rational Design Approach to Developing Second Generation Fabry Disease Treatments , Matthew Metcalf, Molecular and Cellular Biology

Improved Molecular Diagnostics for Soil-Transmitted Helminths , Nils Pilotte, Molecular and Cellular Biology

CHARACTERIZING ADAPTIVE NON-CODING CHANGES IN THE REGULATION OF HUMAN GENE EXPRESSION , Jason Pizzollo, Molecular and Cellular Biology

REDOX SIGNALING IN THE ZEBRAFISH EMBRYO AND IMPLICATIONS FOR ENDOCRINE PANCREAS MORPHOGENESIS , Archit Rastogi, Molecular and Cellular Biology

Liver Kinase B1 isoform expression as a regulator of T cell phenotypic stability , Heather Sherman, Molecular and Cellular Biology

Engineering mesothelin-binding proteins as targeted cancer diagnostics and therapeutics , Allison Rita Sirois, Molecular and Cellular Biology

ACCESSORY GENES CONTRIBUTE TO REWIRING THE TRANSCRIPTIONAL NETWORK IN FUSARIUM OXYSPORUM , He Yang, Molecular and Cellular Biology

The Lon protease integrates protein quality control with DNA damage responses in Caulobacter crescentus , Rilee D. Zeinert, Molecular and Cellular Biology

DE-CODING THE IMPACT OF EVOLVED CHANGES IN GENE EXPRESSION AND CELLULAR PHENOTYPE ON PRIMATE EVOLUTION , Trisha Zintel, Molecular and Cellular Biology

Dissertations from 2019 2019

ENGINEERING OF AN ANTIBODY-CONJUGATED NANOGEL PLATFORM FOR TARGETED DRUG DELIVERY TO T LYMPHOCYTES , Mine Canakci, Molecular and Cellular Biology

ENHANCING NANOPORE BASED BIOSENSENING TECHNOLOGY USING PORE FORMING PROTEINS , Christina M. Chisholm, Molecular and Cellular Biology

CHARACTERIZATION OF β-2-MICROGLOBULIN PRE-AMYLOID OLIGOMERS AND THEIR ROLE IN AMYLOID INHIBITION , Tyler M. Marcinko, Molecular and Cellular Biology

ENVIRONMENTAL RISK FACTORS FOR INFLAMMATORY BOWEL DISEASE: TRICLOSAN AND OTHER CONSUMER ANTIMICROBIALS , Katherine Z. Sanidad, Molecular and Cellular Biology

Let-7 MiRNAs Program The Fate Of CD8 T Cells , Alexandria Wells, Molecular and Cellular Biology

Dissertations from 2018 2018

TARGETED LOCALIZATION OF MICROTUBULE-SEVERING ENZYMES TO CREATE NEW LOCAL TOOLS TO STUDY CELL DIVISION , Siddheshwari Advani, Molecular and Cellular Biology

MECHANISM OF REGULATION OF KINESINS EG5 AND KIF15 BY TPX2 , Sai Keshavan Balchand, Molecular and Cellular Biology

EXAMINING sHSP-SUBSTRATE CAPTURE AND CHAPERONE NETWORK COORDINATION THROUGH CROSS-LINKING , Keith Ballard, Molecular and Cellular Biology

REGULATION OF EXPRESSION OF THE  T CELL WC1 CO-RECEPTOR AND PATTERN RECOGNITION RECEPTOR MULTIGENE FAMILY , Payal Damani-Yokota, Molecular and Cellular Biology

Structural studies of acid alpha glucosidase and Pompe disease , Derrick Deming, Molecular and Cellular Biology

The Role of the Metallochaperone HypA in the Acid Survival and Activities of Nickel Enzymes in Helicobacter pylori , Heidi Hu, Molecular and Cellular Biology

IDENTIFYING FUNCTIONAL COMPONENTS OF THE ENDOPLASMIC RETICULUM QUALITY CONTROL AND DEGRADATION FACTOR EDEM1 , Lydia Lamriben, Molecular and Cellular Biology

Motor Protein Regulation in Mammalian Mitosis , Barbara Mann, Molecular and Cellular Biology

EPIGENETIC REGULATION OF GENOMIC IMPRINTING DURING EARLY MAMMALIAN EMBRYONIC DEVELOPMENT , Chelsea Marcho, Molecular and Cellular Biology

Cadherin-11 Ectodomain Signaling in Cranial Neural Crest Migration , Ketan Mathavan, Molecular and Cellular Biology

STUDIES OF DYNEIN ANCHORING PROTEIN IN BUDDING YEAST SACCHAROMYCES CEREVISIAE , Safia Omer, Molecular and Cellular Biology

Defining and Manipulating the Function of Protein Kinase C-theta in Graft versus Host Responses , Emrah Ilker Ozay, Molecular and Cellular Biology

EXTRINSIC AND INTRINSIC FACTORS IN LIVER DEVELOPMENT , Amrita Palaria, Molecular and Cellular Biology

Studies on the P. aeruginosa T3S translocon assembly: interaction of PopD with membranes , Yuzhou Tang, Molecular and Cellular Biology

CANONICAL NOTCH SIGNALING POSITIVELY REGULATES MIR-155 TRANSCRIPTION IN A NFκB-DEPENDENT MANNER , Joe Torres, Molecular and Cellular Biology

CLPXP FUNCTIONS IN CAULOBACTER AS A UNIVERSAL AND SPECIES-SPECIFIC PROTEASE , Robert Vass, Molecular and Cellular Biology

CHARACTERIZATION OF SHE1 SPINDLE ROLE USING CEULLULAR, BIOCHEMICAL, AND BIOPHYSICAL METHODS , Yili Zhu, Molecular and Cellular Biology

Dissertations from 2017 2017

Notch1 Modification and Signaling in T Helper Cell Differentiation , Karthik Chandiran, Molecular and Cellular Biology

Pore forming protein assembly and the use in nanopore sensing: a study on E. coli proteins ClyA and OmpG , Monifa Fahie, Molecular and Cellular Biology

Adaptors at Work: Regulation of Bacterial Proteolysis by Adaptor Hierarchies , Kamal Joshi, Molecular and Cellular Biology

THE KEY QUESTION IN SYMBIOTIC NITROGEN FIXATION: HOW DOES HOST MAINTAIN A BACTERIAL SYMBIONT? , Onur Oztas, Molecular and Cellular Biology

Dissecting Molecular Pathways that ensure Proper Chromosome Segregation and Cell Division , Anna Ye, Molecular and Cellular Biology

Dissertations from 2016 2016

The Role of NOTCH3 Signaling in T Helper Cell Differentiation and Induction of EAE , Furkan Ayaz, Molecular and Cellular Biology

Studies of Kinetochore Mechanobiology in Drosophila , Stuart Cane, Molecular and Cellular Biology

Investigating the impact of small molecule ligands and the proteostasis network on protein folding inside the cell , Karan Hingorani, Molecular and Cellular Biology

The Cellular Context of Estradiol Regulation in the Zebra Finch Auditory Forebrain , Maaya Ikeda, Molecular and Cellular Biology

Exploring the Impact of the E. coli Proteostasis Network on the Folding Fate of Proteins with Different Intrinsic Biophysical Properties , Kristine Faye R. Pobre, Molecular and Cellular Biology

DNA-Based Epigenetic Changes in Recurrent and Tamoxifen-Resistant Breast Cancer , Kristin E. Williams, Molecular and Cellular Biology

Alternative Methods for the Treatment of Chemo-Resistant Cancers , Kaitlyn Wong, Molecular and Cellular Biology

Inhibition of DNA Methylation in Acquired Tamoxifen-Resistant Breast Cancer: Cell Line Model and Clinical Implications , Stephanie Zimmers, Molecular and Cellular Biology

Dissertations from 2015 2015

Uncovering New Roles for the Cell Surface Protease ADAM13 in Neural Crest Migration , Genevieve Abbruzzese, Molecular and Cellular Biology

Biophysical Characterization of Katanin’s Regulation of Microtubules , Megan E. Bailey, Molecular and Cellular Biology

How to Build a Spinal Cord: Exploring Radial Glial Proliferation and their Contribution to Embryonic Neurogenesis , Kimberly A. Johnson, Molecular and Cellular Biology

NEW INSIGHTS INTO THE ROLE OF THE UDP-GLUCOSE: GLYCOPROTEIN GLUCOSYLTRANSFERASE 1 IN THE ENDOPLASMIC RETICULUM QUALITY CONTROL , Abla Tannous, Molecular and Cellular Biology

Dissertations from 2014 2014

THE ESTRADIOL-INDUCED TRANSCRIPTOME OF THE FEMALE MOUSE ANTEROVENTRAL PERIVENTRICULAR NUCLEUS: MORE THAN JUST A KISS , Leah K. Aggison, Molecular and Cellular Biology

CHARACTERIZATION OF Ca2+ INFLUX PATHWAY(S) DURING MOUSE OOCYTE MATURATION , Banyoon Cheon, Molecular and Cellular Biology

Biophysical Studies of Axonal Transport , Leslie Cyle Conway, Molecular and Cellular Biology

NON-CANONICAL NOTCH SIGNALING REGULATES ACTIVATION AND DIFFERENTIATION OF PERIPHERAL CD4+ T CELLS , Anushka Dongre, Molecular and Cellular Biology

Engineering Probes to Detect Cholesterol Accessibility on Membranes Using Perfringolysin O , Benjamin B. Johnson, Molecular and Cellular Biology

MASS SPECTROMETRY BASED EXPERIMENTAL STRATEGIES TO CHARACTERIZE NATIVE AND NON-NATIVE DISULFIDE BONDS IN CYSTEINE-RICH PROTEIN THERAPEUTICS , Adriana Z. Kita, Molecular and Cellular Biology

STRUCTURAL BIOLOGY AND PHARMACOLOGY OF HUMAN CATHEPSIN A AND NEURAMINIDASE 1 , Nilima Kolli, Molecular and Cellular Biology

NOVEL STRATEGIES TO MODULATE SYNAPTIC COMMUNICATION AND INVESTIGATE THE ROLE OF HDAC6 IN ALZHEIMER’S DISEASE , Kathryne A. Medeiros, Molecular and Cellular Biology

THE IDENTIFICATION AND CHARACTERIZATION OF A GROUP OF ER TPR-CONTAINING ADAPTER PROTEINS , johan C. Sunryd, Molecular and Cellular Biology

Dissertations from 2013 2013

Regulation and Action of Skp2 and Rhoa in Cell and Tumor Models: Investigation into the Molecular Mechanisms Responsible for the Aggressive Phenotype of Triplenegative Breast Cancer , Katrina D. Fagan-Solis, Molecular and Cellular Biology

The Unavoidable Threat of Aggregation: Implications for Folding and Function of a β-Rich Protein , Mylene Hazelle Anne Ferrolino, Molecular and Cellular Biology

The Role of YKL-40 in the Progression of Glioblastoma , Ralph Anthony Francescone, Molecular and Cellular Biology

WC1 Functions as a Co-Receptor and A Pattern Recognition Receptor In Bovine Gamma delta T Cells , Hao-Ting Hsu, Molecular and Cellular Biology

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Home > Life Sciences > Microbiology and Molecular Biology > Theses and Dissertations

Microbiology and Molecular Biology Theses and Dissertations

Theses/dissertations from 2024 2024.

Characterization of Cellular Metabolism Regulation by the Transcription Factor Centromere Binding Factor 1 (Cbf1) , Spencer Ellsworth

Theses/Dissertations from 2023 2023

Elucidating the Architecture of the TclIJN Complex that Converts Cysteine to Thiazoles in the Biosynthesis of Micrococcin , Diana G. Calvopina Chavez

Manipulating and Assaying Chromatin Architecture Around Enhancer Elements in vivo , John Lawrence Carter

Halophilic Genes that Impact Plant Growth in Saline Soils , Mckay A. Meinzer

Characterizing Stress Granule Regulation by PAS Kinase, Ataxin-2 and Ptc6 and Investigating the Lifespan of Covid-19 Virus on Currency , Colleen R. Newey

Changes in RNA Expression of HuT78 Cells Resulting From the HIV-1 Viral Protein R R77Q Mutation , Joshua S. Ramsey

Theses/Dissertations from 2022 2022

Biofilm Characterization and the Potential Role of eDNA in Horizontal Gene Transfer in Hospital and Meat Isolates of Staphylococcus aureus and Their Biofilms , Ashley Lynne Ball

Novel Patterns for Nucleosome Positioning: From in vitro to in vivo , David Andrew Bates

The Effects of Polymorphisms of Viral Protein R of HIV-1 on the Induction of Apoptosis in Primary Cells and the Characterization of Twelve Novel Bacillus anthracis Bacteriophage , Jacob D. Fairholm

Analysis of the Cytopathogenic Effect of Different HIV-1 Vpr Isoforms on Primary Human CD4+ T Cells and a Model Cell Line , Jonatan Josue Fierro Nieves

The Role of Chitinase A in Mastitis-Associated Escherichia coli Pathogenesis , Weston D. Hutchison

Big Data Meta-Analyses of Transcriptional Responses of Human Samples to Orthohantavirus Infection and Shotgun Metagenomics From Crohn's Disease Patients. , John L. Krapohl

An Exploration of Factors that Impact Uptake of Human Papillomavirus Vaccines , David Samuel Redd

Genomic Analysis and Therapeutic Development of Bacteriophages to Treat Bacterial Infections and Parasitic Infestations , Daniel W. Thompson

The Use of Nucleotide Salvage Pathway Enzymes as Suitable Tumor Targets for Antibody-Based and Adoptive Cell Therapies , Edwin J. Velazquez

Comparative Sequence Analysis Elucidates the Evolutionary Patterns of Yersinia pestis in New Mexico over Thirty-Two Years , M. Elizabeth Warren

Regulation of T Cell Activation by the CD5 Co-Receptor and Altered Peptides, Characterization of Thymidine Kinase-Specific Antibodies, and Integrating Genomics Education in Society , Kiara Vaden Whitley

Theses/Dissertations from 2021 2021

Evolution and Selection: From Suppression of Metabolic Deficiencies to Bacteriophage Host Range and Resistance , Daniel Kurt Arens

Identifying Sinorhizobium meliloti Genes that Determine Fitness Outcomes , Alexander B. Benedict

Pushing the Limits of SARS-CoV-2 Survival: How SARS-CoV-2 Responds to Quaternary Ammonium Compounds and Wastewater , Benjamin Hawthorne Ogilvie

Mutations in HIV-1 Vpr Affect Pathogenesis in T-Lymphocytes and Novel Strategies to Contain the Current COVID-19 Pandemic , Antonio Solis Leal

Theses/Dissertations from 2020 2020

Staphylococcus aureus Metal Acquisition in Milk and Mammary Gland Tissue , Shalee Killpack Carlson

Antimicrobial Peptide Development: From Massively Parallel Peptide Sequencing to Bioinformatic Motif Identification , Alexander K. Erikson

A Comparison of Chikungunya Virus Infection, Dissemination, and Cytokine Induction in Human and Murine Macrophages and Characterization of RAG2-/-γc-/- Mice as an Animal Model to Study Neurotropic Chikungunya Disease , Israel Guerrero

The Effects of Immune Regulation and Dysregulation: Helper T Cell Receptor Affinity, Systemic Lupus Erythematosus and Cancer Risk, and Vaccine Hesitancy , Deborah K. Johnson

Identification of Genes that Determine Fitness, Virulence, and Disease Outcomes in Mastitis Associated Eschericia coli , Michael Andrew Olson

Theses/Dissertations from 2019 2019

Investigation of Thymidine Kinase 1 in Cancer Progression , Eliza Esther King Bitter

Ribosomally Synthesized and Post-Translationally Modified Peptides as Potential Scaffolds for Peptide Engineering , Devan Bursey

Bioaerosols Associated with Evaporative Cooler Use in Low-Income Homes in Semi-Arid Climates , Ashlin Elaine Cowger

PAS Kinase and TOR, Controllers of Cell Growth and Proliferation , Brooke Jasmyn Cozzens

Regulation of Immune Cell Activation and Functionby the nBMPp2 Protein andthe CD5 Co-Receptor , Claudia Mercedes Freitas

Characterizing Novel Pathways for Regulation and Function of Ataxin-2 , Elise Spencer Melhado

Interactions Between the Organellar Pol1A, Pol1B, and Twinkle DNA Replication Proteins and Their Role in Plant Organelle DNA Replication , Stewart Anthony Morley

SNFing Glucose to PASs Mitochondrial Dysfunction: The Role of Two Sensory Protein Kinases in Metabolic Diseases , Kai Li Ong

Characterizing the Function of PAS kinase in Cellular Metabolism and Neurodegenerative Disease , Jenny Adele Pape

Isolation, Characterization, and Genomic Comparison of Bacteriophages of Enterobacteriales Order , Ruchira Sharma

Isolation, Genetic Characterization and Clinical Application of Bacteriophages of Pathogenic Bacterial Species , Trever Leon Thurgood

Investigation of Therapeutic Immune Cell Metabolism , Josephine Anna Tueller

Theses/Dissertations from 2018 2018

Innate Immune Cell Phenotypes Are Dictated by Distinct Epigenetic Reprogramming , Kevin Douglas Adams

Bacteriophages for Treating American Foulbrood and the Neutralization of Paenibacillus larvae Spores , Thomas Scott Brady

Methods for Detection of and Therapy for Carbapenem-Resistant Enterobacteriaceae , Olivia Tateoka Brown

The Diversity Found Among Carbapenem-Resistant Bacteria , Galen Edward Card

Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides , Emma Kay Dallon

Gut Microbiota Regulates the Interplay Between Diet and Genetics to Influence Insulin Resistance , Jeralyn Jones Franson

The Antimicrobial Properties of Honey and Their Effect on Pathogenic Bacteria , Shreena Himanshu Mody

The Ability of Novel Phage to Infect Virulent Bacillus anthracis Isolates , Hyrum Smith Shumway

Galleria Mellonella as an Alternate Infection Model for Burkholderia Species and a Comparison of Suspension and Surface Test Methods for Evaluating Sporicidal Efficacy , Joseph D. Thiriot

The Clinical Significance of HPRT as a Diagnostic and Therapeutic Biomarker for Hematological and Solid Malignancies , Michelle Hannah Townsend

Biomarker Analysis and Clinical Relevance of Thymidine Kinase 1 in Solid and Hematological Malignancies , Evita Giraldez Weagel

Hospital and meat associated Staphylococcus aureus and Their Biofilm Characteristics , Trevor Michael Wienclaw

Theses/Dissertations from 2017 2017

Comparison of Cytokine Expression and Bacterial Growth During Periparturient and Mid Lactation Mastitis in a Mouse Model , Rhonda Nicole Chronis

Influence of Epstein-Barr Virus on Systemic Lupus Erythematosus Disease Development and the Role of Depression on Disease Progression , Caleb Cornaby

The Effects of Nucleosome Positioning and Chromatin Architecture on Transgene Expression , Colton E. Kempton

Phosphate Signaling Through Alternate Conformations of the PstSCAB Phosphate Transporter , Ramesh Krishna Vuppada

Acetobacter fabarum Genes Influencing Drosophila melanogaster Phenotypes , Kylie MaKay White

Theses/Dissertations from 2016 2016

The Path to Understanding Salt Tolerance: Global Profiling of Genes Using Transcriptomics of the Halophyte Suaeda fruticosa , Joann Diray Arce

Genetic and Biochemical Analysis of the Micrococcin Biosynthetic Pathway , Philip Ross Bennallack

Characterizing Interaction Between PASK and PBP1/ ATXN2 to Regulate Cell Growth and Proliferation , Nidhi Rajan Choksi

The Activity of Alkaline Glutaraldehyde Against Bacterial Endospores and Select Non-Enveloped Viruses , Justen Thalmus Despain

The Role of Viral Interleukin-6 in Tumor Development of Kaposi's Sarcoma-Associated Herpesvirus Lymphomas , Rebecca A. Fullwood

The Role of the Transcriptional Antiterminator RfaH in Lipopolysaccharide Synthesis, Resistance to Antimicrobial Peptides, and Virulence of Yersinia pseudotuberculosis and Yersinia pestis , Jared Michael Hoffman

A CryAB Interactome Reveals Clientele Specificity and Dysfunction of Mutants Associated with Human Disease , Whitney Katherine Hoopes

The pmrHFIJKLM Operon in Yersinia pseudotuberculosis Enhances Resistance to CCL28 and Promotes Phagocytic Engulfment by Neutrophils , Lauren Elizabeth Johnson

Characterization of Five Brevibacillus Bacteriophages and Their Genomes , Michael Allen Sheflo

Analysis of Nucleosome Isolation and Recovery: From In Silico Invitrosomes to In Vivo Nucleosomes , Collin Brendan Skousen

Human Herpesvirus 6A Infection and Immunopathogenesis in Humanized Rag2 -/-γc-/- Mice and Relevance to HIV/AIDS and Autoimmunity , Anne Tanner

Theses/Dissertations from 2015 2015

Identifying and Characterizing Yeast PAS Kinase 1 Substrates Reveals Regulation of Mitochondrial and Cell Growth Pathways , Desiree DeMille

The Detection and Molecular Evolution of Francisella tularensis Subspecies , Mark K. Gunnell

Isolation and Host Range of Staphylococcus aureus Bacteriophages and Use for Decontamination of Fomites , Kyle C. Jensen

The Antioxidant and DNA Repair Capacities of Resveratrol, Piceatannol, and Pterostilbene , Justin Ryan Livingston

High Salinity Stabilizes Bacterial Community Composition and Activity Through Time , Tylan Wayne Magnusson

Advancing Phage Genomics and Honeybee Health Through Discovery and Characterization of Paenibacillaceae Bacteriophages , Bryan Douglas Merrill

Specialized Replication Operons Control Rhizobial Plasmid Copy Number in Developing Symbiotic Cells , Clarice Lorraine Perry

Gene Networks Involved in Competitive Root Colonization and Nodulation in the Sinorhizobium meliloti-Medicago truncatula Symbiosis , Ryan D. VanYperen

Theses/Dissertations from 2014 2014

Snf1 Mediated Phosphorylation and Activation of PAS Kinase , Bryan D. Badal

Studies of PhoU in Escherichia coli: Metal Binding, Dimerization,Protein/Protein Interactions, and a Signaling Complex Model , Stewart G. Gardner

Pharmacologic Immunomodulation of Macrophage Activation by Caffeine , Ryan Perry Steck

Analysis of Nucleosome Mobility, Fragility, and Recovery: From Embryonic Stem Cells to Invitrosomes , Ashley Nicolle Wright

Enhancing Protein and Enzyme Stability Through Rationally Engineered Site-Specific Immobilization Utilizing Non-Canonical Amino Acids , Jeffrey Chun Wu

Theses/Dissertations from 2013 2013

Thymidine Kinase 1: Diagnostic and Prognostic Significance in Malignancy , Melissa Marie Alegre

Promoter Polymorphisms in Interferon Regulatory Factor 5 , Daniel N. Clark

Modulators of Symbiotic Outcome in Sinorhizobium meliloti , Matthew B. Crook

Evidences for Protein-Protein Interactions Between PstB and PhoU in the Phosphate Signaling Complex of Escherichia coli , Kristine Dawn Johns

Identification of the Binding Partners for HspB2 and CryAB Reveals Myofibril and Mitochondrial Protein Interactions and Non-Redundant Roles for Small Heat Shock Proteins , Kelsey Murphey Langston

A Quadruplex Real-Time PCR Assay for the Rapid Detection and Differentiation of the Burkholderia pseudomallei Complex: B. mallei , B. pseudomallei , and B. thailandensis , Chinn-woan Lowe

The Role of Nuclear BMP2 in the Cell Cycle and Tumorigenesis , Brandt Alan Nichols

Nuclear BMP2 and the Immune Response , Daniel S. Olsen

Hypersaline Lake Environments Exhibit Reduced Microbial Dormancy , Joshua Christopher Vert

Theses/Dissertations from 2012 2012

Characterization of the Cellular and Organellar Dynamics that Occur with a Partial Depletion of Mitochondrial DNA when Arabidopsis Organellar DNA Polymerase IB is Mutated , John D. Cupp

Effect of Antioxidants and Oxidative Stress on Different Cancer Cell Types , Gaytri Devi Gupta Elera

Effects of Chemical Stimulation and Tumor Co-Incubation on Macrophage Activation and Aggressiveness, Measured Through Phagocytosis and Respiratory Burst , Bo Marcus Gustafsson

Loss of the Lipopolysaccharide Core Biosynthesis rfaD Gene Increases Antimicrobial Chemokine Binding and Bacterial Susceptibility to CCL28 and Polymyxin: A Model for Understanding the Interface of Antimicrobial Chemokines and Bacterial Host Defense Avoidance Mechanisms , Cynthia S. Lew

Partial Characterization of the Antimicrobial Activity of CCL28 , Bin Liu

Characterizing the Role of HspB2 in Cardiac Metabolism and Muscle Structure Using Yeast and Mammalian Systems , Jonathan Paul Neubert

Humanized Mice as a Model to Study Human Viral Pathogenesis and Novel Antiviral Drugs , Freddy Mauricio Sanchez Tumbaco

Transgene Delivery via Microelectromechanical Systems , Aubrey Marie Mueller Wilson

Theses/Dissertations from 2011 2011

Antioxidants in Cancer Research and Prevention: Assay Comparison, Structure-Function Analysis, and Food Product Analysis , Andrew Robert Garrett

Characterization of the Role Nuclear Bmp2 (nBmp2) Plays in Regulating Gene Expression , Fialka Grigorova

Theses/Dissertations from 2010 2010

Effects of Diabetic State and Gender on Pro-Inflammatory Cytokine Secretion by Human Macrophages Infected with Burkholderia pseudomallei , Annette J. Blam

Organellar DNA Polymerases Gamma I and II in Arabidopsis thaliana , Jeffrey M. Brammer

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Department of Biochemistry and Molecular Biology Theses and Dissertations

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This collection contains theses and dissertations submitted by graduate students under the Department of Biochemistry and Molecular Biology for either a Master of Science degree or a Doctor of Philosophy degree.

Recent Submissions

OPTIMIZED ISOLATION AND QUANTIFICATION OF IN VIVO DISTRIBUTION OF EXOSOMES FOR POTENTIAL TARGETED THERANOSTIC APPLICATION

THE ROLE OF GPR109A IN REGULATION OF RETINAL ANGIOGENESIS AND BLOOD-RETINAL BARRIER AS A POTENTIAL THERAPEUTIC TARGET IN DIABETIC RETINOPATHY

A Molecular Basis of Chemoresistance in Bladder Cancer

Osteopontin as a novel immune checkpoint, gene regulation by the putative campylobacter jejuni diguanylate cyclase cbrr, glycosaminoglycans, chondroitinase, and molecular subtypes in bladder cancer.

HLA-G DIMER PROLONGS KIDNEY ALLOGRAFT SURVIVAL BY INHIBITING CD8+T CELL ACTIVATION AND GRANZYME B EXPRESSION

Investigating the Role of the Hdac3 Co-Repressor Complex in Glucocorticoid Signaling-Mediated Bone Marrow Lipid Storage with Age

CHOLINESTERASE INHIBITOR TOXICITY: MECHANISTIC STUDIES AND THERAPEUTIC STRATEGIES FOCUSED ON AXONAL TRANSPORT

Primary Tumor-Induced Immunity Is Suppressed By Surgery-Induced Inflammation In The Presence Of Residual Tumor Cells

DISCOVERY AND VALIDATION OF A NOVEL NEUTROPHIL ACTIVATION MARKER ASSOCIATED WITH OBESITY

Role of arginase in obesity-induced visceral adipose tissue dysregulation and endothelial dysfunction.

DNA METHYLATION AS A KEY PLAYER IN INFLAMMATION-MEDIATED COLON TUMORIGENESIS

The c-MYC oncogene deregulates global DNA methylation and hydroxymethylation to control genome-wide gene expression for tumor maintenance in leukemia/lymphoma

The Role of GPR109A in NAD+ Metabolism in Aging RPE

Immune regulation of tumor cell plasticity: A promising molecular target in breast cancer metastasis

THE TUMOR SECRETORY FACTOR ZAG PROMOTES WHITE ADIPOSE TISSUE BROWNING AND ENERGY WASTING IN CACHEXIA

Canonical wnt signaling in antigen presenting cells regulates microbiota-induced inflammation and immune cell homeostasis in the colon.

CXCR2 EXPRESSING TUMOR CELLS DRIVE VASCULAR MIMICRY IN ANTI-ANGIOGENIC THERAPY RESISTANT GLIOBLASTOMA

MECHANISM OF 12/15 LIPOXYGENASE-INDUCED RETINAL MICROVASCULAR DYSFUNCTION IN DIABETIC RETINOPATHY

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• Recent Ph.D. Dissertation Research Topics

August of 2017

Novel Cell Surface Anchoring Mechanism of Prokaryotic Secreted Protein - Mohd Farid Abdul Halim, Ph.D.

The Genetic Architecture Underlying Rapid Seasonal Evolution in Natural Populations of Drosophila melanogaster - Emily Behrman, Ph.D.

Developmental Functions of MIR156 and MIR157 in Arabidopsis - Jia He, Ph.D.

Dedifferentiation of Cytotoxic Lympthocyte into Central Memory CD8+T Cells:  Lessons from Antiviral T-Stem Cells on the Architecture of Aging and Immunotherapy - Jonathan Johnnidis, Ph.D.

Class of 2017

Proteolytic Activation by ADAMTS3 Defines Distinct Mechanisms of Lymphangiogenesis Mediated by VEGFC and VEGFD - Hung Bui, Ph.D.

Transcript Diversity in the Protozoan Parasite Toxoplasma Gondii - Maria Diaz, Ph.D.

Plant-soil Feedback and Succession in Heavy Metal Soils - Lee Dietterich, Ph.D.

Genome Editing Approach to Uncover Microtubule-Actin Crosslinking Factor (MACF1) Essential Domains in Establishing Oocyte Polarity and Nuclear Positioning - Matias Escobar, Ph.D.

Investigation of the Spatiotemporal Dynamics of cAMP and PKA Signaling and the Role of HCN4 Subunits in Anxiety-Related Behavior and Memory - Vincent Luczak, Ph.D.

The C-REL Transcription Factor Controls Metabolism and Proliferation of Human T Cells - George Luo, Ph.D.

Space, Time and Change: Investigations in Soil Bacterial Diversity in the Montane Steppe of Mongalia - Aurora MacRae-Crerar, Ph.D .

Population Genetics of Borrelia Burgdorferi in the Eastern and Midwestern United States - Stephanie Seifert, Ph.D.

Hypermutability in Asexuals: Investigating the Effects of Deleterious Mutations - Tanya Singh, Ph.D.  

Class of 2016

Molecular and Cellular Approaches Toward Understanding Dynein-Driven Motility - Swathi Ayloo, Ph.D.

Cell Biology of Cheating - Mechanisms of Chromosomes Segregation during Female Meiosis -  Lukas Chmatal, Ph.D.

Detecting Selection on Noncoding Nucleotide Variations: Methods and Applications -  Yang Ding, Ph.D.

Short Term Adult Plasticity in Drosophila melanogaster and its Role in Climatic A daptation - Vinayak Mathur. Ph.D.

A Study of the Role of Gata6 in Definitive Endoderm Specification and β Cell Functionality by Genome Engineering of the Pluripotent Stem Cells  -  Amita Tiyaboonchai, Ph.D.

Phenotypic Variation in the Dogwhelk, Nucella lapillus:  an Integration of Ecology, Karyotype and Phenotypic Plasticity -  Katie Vazquez, Ph.D.

Genomic, Evolutionary and Functional Analyses of Diapause in  Drosophila melanogster -  Xiaqing Zhao, Ph.D.

Selective Forces that Shape the VLS Antigenic Variation System in Borrelia Burgdorferi -  Wei  Zhou, Ph.D.

Class of 2015

A Role of SWI/SNF Complex in ABA-dependent Drought Responsive Gene Expression in Arabidopsis thaliana - Soon Ki Han, Ph.D.

Mechanisms Underlying the Role of Drosophila TIMELESS in the Molecular Cl ock and in the Circadian Response to Light - A Reum Jang, Ph.D.

The Genetic Basis of Phenotypic Plasticity in Natural Populations of Drosophila - Katherine O'Brien, Ph.D.

Spatial and Temporal Variation in Wild Pollination Services in the Mongolian Steppe - Dan Song, Ph.D.

 Promoting T-snare Binary Complex Assembly and Membrane Fusion by the Exocyst Protein Sec3 - Peng Yue, Ph.D.

Novel Regulators in the Germline Stem Cell Niche of Drosophila Testis - Qi Zheng, Ph.D.

Class of 2014

Change and Impact of microRNA Modification with Age in Drosophila melanogaster - Masashi Abe, Ph.D.

Mitotic Kinases in Meiosis - Olga Davydenko, Ph.D.

Higher-Order Chromatin Organization in Hematopoietic Transcription - Wulan Deng, Ph.D.

Effects of Temperature on Global Gene Expression in Natural Strains of Budding Yeast - Hoa Giang, Ph.D.

Regulators of Mouse and Human Beta Cell Proliferation - Yang Jiao, Ph.D.

Trial-by-Trial Coding of Instructive Signals in the Cerebellum:  Insights from Eyeblink Conditioning in Mice - Farzaneh Najafi, Ph.D. 

The Molecular Mechanism of Synaptic Tagging and Capture - Jung Whan Park, Ph.D.

Dynamics and Fate of the Inner Membrane complex in Toxoplasma gondii - Dinkorma Toure Ouologuem, Ph.D.

Regulation of Adipose Tissue Development and Function by Nuclear Receptors PPARg and Rev-erba - Fenfen Wang, Ph.D.

Towards a Better Understanding of Nonstructural Carbohydrate Storage and Carbon Limitation in Trees - Erin Wiley, Ph.D.

Exo70 Generates Membrane Curvature for Morphogenesis and Cell Migration - Yuting Zhao, Ph.D.

Class of 2013

Higher-order Chromatin Organization in Hematopoietic Transcription -  Wulan Deng, Ph.D.

Multiscale Analyses of the Population Structure of Triatoma infestans in an Urban Area  -  Erica Foley, Ph.D.

The Impact of Terrestrial Dissolved Organic Carbon on Stream Ecosystem Function through an Investigation of Hydrologic Sources in White Clay Creek, PA -  Christine McLaughlin, Ph.D.

  Biology of Respiratory and Cardiac Functional Deterioration in a Translational Model for Duchenne Muscular Dystrophy -  Andrew Mead, Ph.D.

  Species-Specific Effects on the Oxygen Isotope Ratio of Tree-Ring Cellulose -  Xin Song, Ph.D.

  The interplay between Lewy body-like alpha-synuclein aggregates and protein degradation pathways in a cell-based model of Parkinson’s disease  -  Selcuk Tanik, Ph.D.

  Structure and Function of H. volcanii flagella -  Manuela Tripepi, Ph.D.

Class of 2012

Investigation of the Methane Cycle and Associated Microbial Community of a Pine Forest Soil  - Emma Aronson, Ph.D.

A Role for Late Meristem Identity2 in the Reproductive Development of Arabidopsis  - Jennifer Pastore, Ph.D.

The Molecular Mechanism of Vegetation Phase Change: Regulation of miR156 Expression and Action  - Li Yang, Ph.D.

Meiotic Origins of Maternal Age-Related Aneuploidy  - Teresa Chiang, Ph.D.

Decoding Cytochrome C Oxidase Biogenesis: New Insights into Copper Trafficking  - Nursel Ekici, Ph.D.

Evolution of Molecular Function in Mammalian Neurons  - Chantal Francis, Ph.D.

The Cytoskeletal Mechanisms of Cell-Cell Junction Formation in Endothelial Cells  - Matthew Hoelzle, Ph.D.

Microrna Precursor Programmed Silencing Complex Assembly and Regulatory Pathways in Mammals  - Xuhang Liu, Ph.D.

The Role of EPAC Signaling in Memory Consolidation and Sleep Deprivation  - Nan Ma, Ph.D.

Behavior and Socioendocrinology of Adult and Male Rhesus Macaques of Cayo Santiago  - Maria Rakhovskaya, Ph.D.

Molecular Insight into Mechanisms of CAG-Repeat RNA Toxicity in Polyglutamine Disease from Drosophila  - Shin-Yi Shieh, Ph.D.

The Evolution of a High Mutation Rate and Declining Fitness in Asexual Populations  - Christopher Gentile Ph.D.

Selection on Mutators in Experimental Saccharomyces Cerevisiae Populations: Recombination, Clonal Interference, and the Evolution of Mutation Rates  - Yevgeniy Raynes, Ph.D.

Class of 2011

Socioecology, Acoustic Communication and Demography of Asian Elephants in Sri Lanka  - Shermin deSilva, Ph.D.

The Role of Exocyst in Exocytosis and Cell Migration  - Jianglan Liu, Ph.D.

YY1 Controls Immunoglobulin Class Switch Recombination and Nuclear Aid Levels  - Kristina Zaprazna, Ph.D.

Mechanisms of HDAC2 Function in the Regulation of Adult Cardiac Hypertrophy and Embryonic Myocyte Proliferation  - Wenting Zhu, Ph.D.

Actin-Independent Mechanisms of Targeting Formin mDIA2 to the Plasma Membrane  - Roman Gorelik, Ph.D.

Roles of Protein Factors in Regulation of Imprinted Gene Expression  - Shu Lin, Ph.D.

Functional Plasticity of Hippocampal Glutamatergic Synapses in Development and Disease  - Xiaoyu Peng, Ph.D.

Physiological Bases of Plant Shrinkage and its Demographic Implications  - Roberto Salguero-Gomez, Ph.D.

Class of 2010  

Unplugged/Musk Signaling Coordinates Pre- and Post-synaptic Development at the Neuromuscular Junction  - Lili Jing, PhD

Spatially Varying Selection on Shell Color Phenotype in the Flat Periwinkle Littorina Obtusata  - Megan Phifer-Rixey, PhD

Niche Partitioning Among Arbuscular Mycorrhizal Fungi and Consequences for Host Plant Performance  - Jennifer H. Doherty, PhD

Microrna Pathways Modulate Aging and Neurodegenerative Diseases  - Nan Liu, PhD

The Rab3A Locus: Studies of Neuronal Expression and Effects on Behavior  - Rui Liu, PhD

The Evolutionary Genetics of Life History in Drosophila Melanogaster  - Annalise Bloss Paaby, PhD

Using Evolutionary Genomics to Elucidate Parasite Biology and Host-Pathogen Interactions  - Lucia Peixoto, PhD

Class of 2009  

Characterizing the Role of the Exocyst Component EX070 in Exocytosis and Actin Dynamics in Budding Yeast Saccharomyces Cerevisiae  - Bing He, PhD

Slit Down-Regulates CRGAP Function to Mediate Axon Repulsion in the Embryonic Drosphila CNS  - Ming Li, PhD

Molecular Basis of Chve Function in Sugar Binding and Virulence in Agrobacterium Tumefaciens  - Fanglian He, PhD

Spatial and Temporal Variation in Streambed Bacterial Community Composition and Correlation with Einvironmental Variables and Disturbance  - Karen Rowley Hogan, PhD

Squint, The Arabidopsis   Ortholog of Cyclophilin40, Affects RNA Silencing  - Michael Smith, PhD

Spatial Regulation of CDC42 by its GTPase-Activating Proteins RGA1 and BEM2 in Budding Yeast Saccharomyces Cerevisiae  - Zongtian Tong, PhD

Class of 2008

Molecular and Cellular Mechanism of Axon Guidance During Visual System Development in Zebrafis h - Michael Gyda, PhD

Phylogeny and Evolution of Prokaryotic Genomes  - Fan Ge, PhD

The Role of the Exocyst Complex in Polarized Exocytosis  - Puyue Wang, PhD

Evolutionary Ecology of Host Plant Use by an Insect Herbivore in a Highly Seasonal Neotropical Dry Forest  - Salvatore Agosta, PhD

Variation Among Dogwhelks (Nucella lapillus) Modifies Predator-prey Interactions within Intertidal Assemblages  - Jonathan Fisher, PhD

Peeking into the Black Box: The Structure and Function of Soil Microbial Communities in Response to Increasing Nitrogen Availability  - Richard Lucas, PhD

Taxonomic and Functional Diversity of AM Fungi in Serpentine and Prairie Grasslands  - Baoming Ji, PhD

Dissecting Different Modules of Cytochrome C Maturation in Rhodobacter Capsulatu s - Serdar Turkarslan, PhD

Role of Exocytosis in Cell Polarization  - Xiaoli Sun, PhD

Class of 2007

The Analysis of the PI3K/AKT Pathway in Human Ovarian Cancer with a Combination of Genomic and Genetic Appoaches  - Jia Huang, PhD

Examination of the Twin-Arginine Translocation Pathway in Bacteria and Archaea  - Kieran Dilks, PhD

Spatial Orientation in Columbian Ground Squirrels (Spermophilus Columbianus)  - Anna Vlasak, PhD

From Protein to RNA: Study of SCA3 Polyglutamine Disease in Drosophila   - Lingbo Li, PhD

Novel Insignts Into Vir Gene Regulation in Agrobacterium Tumefaciens  - Gauri Nair, PhD

Spatial and Genetic Patterns of Crane Flies (Diptera: Tipuloidea) from Lake Hovsgol Region, Mongolia  - Bazartseren Boldgiv, PhD

Aging in Plants at Multiple Scales  - Lori Spindler, PhD

Metabolic Pool, Metabolic Rates and Diapause in Drosophila Melanogster  - Christine Wills, PhD

Phylogenetics and Diversification of the Neotropical Serrasalminae (Ostariophysi: Characiformes)  - Wasila Dahdal, PhD

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About the PhD in Biochemistry and Molecular Biology Program

In the Biochemistry and Molecular Biology PhD program, faculty, and students work together to increase knowledge of the biochemical and molecular bases of normal and abnormal cellular processes. Our program trains students to be successful independent scientists and gives them the knowledge, research training, and leadership skills to continue to provide new insights into the biomedical issues that have a profound impact on public health.

Students engage in a rigorous course curriculum and a range of structured and informal activities outside the classroom and lab to build their skills. They will pursue their thesis research in the lab of one of our over forty training faculty across the Johns Hopkins Bloomberg School of Public Health and the Johns Hopkins School of Medicine.

Visit our dedicated PhD program website to learn more about the diverse research training opportunities of the program.

PhD in Biochemistry and Molecular Biology Program Highlights

Our position within the School of Public Health provides a unique setting in which students learn how biochemistry, molecular biology, physical chemistry, cell biology, and genetics can be used to solve significant problems in public health and medicine. Our program offers:

• Training faculty from across the School of Public Health and the School of Medicine
• A strong grounding in the science of biomedical and public health research through a core curriculum that includes courses taught by leading experts from the Schools of Public Health and Medicine
• Training outside the lab and classroom in key skills such as communications and leadership
• Opportunities to build strong communications skills through a range of speaking venues including journal club, research colloquium, department retreats, and national meetings
• Access to the Johns Hopkins School of Medicine Professional Development and Career Office , offering excellent career services and professional development,  including the BMB-required OPTIONS program, a guided process of career exploration for paths from medicine to biotech to academia and beyond for careers paths from medicine to biotech to academia and beyond
• Opportunities to participate in community service and outreach, with a focus on our East Baltimore neighborhoods, through the Johns Hopkins University community engagement and service-learning center, SOURCE

Training faculty across the School of Public Health and the School of Medicine

Schools that students can take courses in: Public Health, Arts & Sciences, Medicine, and Engineering

Two-month rotations in the first year prior to selecting thesis lab

Average number of incoming students in the BMB PhD degree program each year

What Can You Do With a PhD In Biochemistry And Molecular Biology?

The Biochemistry and Molecular Biology PhD program prepares students for a range of biomedical and health sciences careers, including in academia, industry, policy, and beyond. Visit the Graduate Employment Outcomes Dashboard to learn about Bloomberg School graduates' employment status, sector, and salaries.

Sample Careers

• Research Scientist
• Science Policy Adviser
• Biotech Executive
• Senior Scientist
• Patent Lawyer
• Science Policy Analyst/Advocate
• Science Writer/Journalist
• Biological Sciences Teacher

Topic Areas

The BMB PhD program faculty conduct research to gain new insights into the cellular and molecular mechanisms underlying normal and abnormal cellular processes, and their relevance as targets for improving health and treating disease. Our training program places particular emphasis on mechanistic approaches to research problems.

Common topic areas within our faculty's diverse research interests include:

• Biophysics and Structural Biology
• Cancer Biology
• Chemical Biology and Proteomics
• Cell Biology
• Cellular Stress and Cell Signaling
• Genetics, Genomics, and Gene Regulation
• Immunology and Infectious Diseases
• Translational Research

Curriculum for the PhD in Biochemistry and Molecular Biology

The BMB PhD offers students a rigorous course curriculum, including a set of common core classes from the Schools of Public Health and Medicine. A rich array of seminar programs and journal clubs are also available to all students.

Browse an overview of the requirements for this PhD program in the JHU  Academic Catalogue  and explore all course offerings in the Bloomberg School  Course Directory .

Courses in core curriculum

Minimum elective credits

Seminars on current research presented by experts from across Johns Hopkins and other biomedical research institutions

Courses available across Johns Hopkins Schools of Public Health, Medicine, and Arts and Sciences

Admissions Requirements

For the general admissions requirements see our How to Apply page. The specific program also requires:

Prior Work Experience

Laboratory research experience (from academia, industry, etc.) is required

Prior Coursework

Strong background in the sciences, particularly in chemistry, biochemistry, or biology

Standardized Test Scores

Standardized test scores (GRE) are optional for this program. The admissions committee will make no assumptions if a standardized test score is omitted from an application, but will require evidence of quantitative/analytical ability through other application components such as academic transcripts and/or supplemental questions.  Applications will be reviewed holistically based on all application components.

Program Faculty Spotlight

Ashani T. Weeraratna

Ashi Weeraratna, PhD, studies how cancer cells move to distant sites and how changes in the normal cells around a tumor contribute to their movement, especially as we age.

Michael J. Matunis

Michael Matunis, PhD, studies how protein modification by SUMO—the small ubiquitin-related modifier—drives changes in key cellular pathways from stress response to DNA repair.

Jennifer M. Kavran

Jennifer Kavran, PhD, MS, MPhil, is a biophysicist who investigates how cells communicate with each other and their environment.

Danfeng Cai

Danfeng Cai, PhD, combines advanced microscopy, genomics, and proteomics to tease out the functions of protein condensates in cells, with a focus on cancer.

Vivien Thomas PhD Scholars

The  Vivien Thomas Scholars Initiative (VTSI)  is an endowed fellowship program at Johns Hopkins for PhD students in STEM fields. It provides full tuition, stipend, and benefits while also providing targeted mentoring, networking, community, and professional development opportunities. Students who have attended a historically Black college and university (HBCU) or other minority serving institution (MSI) for undergraduate study are eligible to apply. To be considered for the VTSI, you will need to submit a SOPHAS application, VTSI supplementary materials, and all supporting documents (letters, transcripts, and test scores) by December 1 , 202 3 . VTSI applicants are eligible for an application fee waiver , but the fee waiver must be requested by November 15, 202 3 and prior to submission of the SOPHAS application.

All full-time PhD students receive the following support for all years of the program: full tuition and fees, individual health insurance, University Health Services fee, vision insurance, dental insurance, and a stipend for living expenses for students who remain in good academic standing. PhD students are required to serve as a teaching assistant for at least one term, in either their 2nd or 3rd year.

Need-Based Relocation Grants Students who  are admitted to PhD programs at JHU starting in Fall 2023 or beyond can apply to receive a $1500 need-based grant to offset the costs of relocating to be able to attend JHU.   These grants provide funding to a portion of incoming students who, without this money, may otherwise not be able to afford to relocate to JHU for their PhD program. This is not a merit-based grant. Applications will be evaluated solely based on financial need.  View more information about the need-based relocation grants for PhD students .

Questions about the program? We're happy to help.

Mike Matunis, PhD PhD Program Director

Roza Selimyan , PhD BMB Executive Director for Academic Affairs and Education Programs

Erika Vaitekunas Administrative Specialist

[email protected]

Compare Programs

• Check out similar programs at the Bloomberg School to find the best fit.
• Master of Science (ScM), Offered by BMB
• Master of Health Science (MHS), Offered by BMB
• Master of Health Science (MHS), Offered by MMI
• Doctor of Philosophy (PhD), Offered by MMI
• Master of Health Science (MHS) in Environmental Health
• Doctor of Philosophy (PhD) in Environmental Health

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• PhD in Molecular Biology, Cell Biology & Biochemistry

The PhD in Molecular Biology, Cell Biology & Biochemistry (MCBB) is designed to build a solid foundation in related fields in the molecular biosciences through coursework and seminars, and to develop the skills for achieving cutting-edge research accomplishments. The program is based on an interdisciplinary environment and a breadth of approaches and biological interests, including faculty from several departments (e.g., Biology, Biomedical Engineering, Chemistry, Health Sciences). The primary goal of the degree is to prepare students for successful careers in academia, research institutions, or industry (e.g., biotechnology and pharmaceutical industries), or in government as policymakers. The program accepts applicants with degrees in related disciplines where the focus has been on biology, chemistry, biotechnology, biochemistry, or a relevant field.

Learning Outcomes

• Demonstrate academic mastery in the interrelated biological disciplines encompassing at least two of three biological areas: 1) molecular biology, 2) cell biology, and 3) biochemistry, plus in computational analysis.
• Achieve research expertise and complete a body of original research that advances a specific field of study in the molecular biosciences.
• Follow the ethics for appropriate behavior in the scientific discipline.
• Be able to teach and promote the field of biological sciences.
• Be prepared to enter the job market.

Course Requirements

For details on requirements regarding grade point averages, annual reports, and responsible conduct in research, please see the MCBB graduate program guide .

A total of 64 credits is required. Of these, a minimum of 32 credits must derive from lecture or seminar courses, and a minimum of 12 credits from elective courses. Remaining coursework normally consists of research credits. Incoming students with previous graduate credits, including from a master’s degree at a different institution, may apply to transfer course credits toward the PhD degree. Course requirements are as follows:

• CAS MB 583 Progress in Cell & Molecular Biology (first semester)
• CAS MB 584 Progress in Cell & Molecular Biology (second semester)
• GRS BI 735 Advanced Cell Biology
• GRS BI 753 Advanced Molecular Biology
• GRS MB 697 A Bridge to Knowledge: A Practical Seminar for First-Year Graduate Students
• GRS MB 721 Graduate Biochemistry or MB 722 Advanced Biochemistry
• A graduate-level quantitative course from a list of options
• 12 credits in elective courses

Language Requirement

There is no foreign language requirement for this degree.

Qualifying Examination

This examination is designed to test the student’s general knowledge encompassing molecular biology, cell biology, and biochemistry, as well as the student’s grasp of the proposed research project and ability to synthesize findings and propose and experimentally test hypotheses. It consists of an oral qualifying exam where the student will defend a written research proposal. The exam is administered by a committee of no fewer than five faculty, at least four of whom must be members of the MCBB program (including the thesis advisor). The qualifying exam should be completed within 2.5 years of matriculation into the MCBB PhD program. Any student failing this examination, at the discretion of the examination committee, may have the opportunity to take it again. Failure of the second examination will be grounds for automatic dismissal from the PhD program and the loss of any further financial aid, although the student may still be eligible for the MA provided that those degree requirements are met.

Dissertation and Final Oral Examination

Candidates shall demonstrate their abilities for independent study in a dissertation that presents original research or creative scholarship. A prospectus for the dissertation must be approved by the readers, the Director of Graduate Studies, and the Department Chair/Program Director. Candidates must pass a final oral examination in which they defend their dissertation as a valuable contribution to knowledge in their field and demonstrate a mastery of their field of specialization in relation to their dissertation. All portions of the dissertation and final oral examination must be completed as outlined in the GRS General Requirements for the Doctor of Philosophy Degree .

Teaching Requirement

The PhD program requires a minimum of two semesters of teaching during a graduate student’s career.

Students electing to leave the PhD program may leave with a master’s degree if they have completed their core course requirements, have obtained 32 credits, and have passed their Qualifying Exam, or have otherwise satisfied the requirements of the master’s degree .

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Ph.D. Program

The training for a Ph.D. in Biology is focused on helping students achieve their goals of being a successful research scientist and teacher, at the highest level. Students work closely with an established advisor and meet regularly with a committee of faculty members to facilitate their progress. The Biology Ph.D. program is part of the larger Biosciences community at Stanford, which includes doctorate programs in the basic science departments at Stanford Medical School. 

There are two tracks within the Biology Ph.D. program:

• Cell, Molecular and Organismal Biology
• Ecology and Evolution

(Previously a part of the Department of Biology Hopkins Marine Station is now a part of the Oceans Department within  Stanford Doerr School of Sustainability )

All  tracks are focused on excellence in research and teaching in their respective areas; where there are differences between the tracks, they are indicated in the links below. 

Requirements & Forms

Dissertation defense, cellular and molecular biology training program, stanford biology ph.d. preview program, career development resources.

Thesis Defense

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• Thesis Advisor Responsibilities More
• Thesis Student Responsibilities More
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• Curric. Certification More
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• Conduct More

The Thesis Defense is a presentation of the student’s independent research topic, an overview of the experiments completed, and a summary of the analyzed data and results. Most students are able to complete their PhD within five to six years after entering the CMB Program. The Thesis Defense must be completed within five years after completion of the Preliminary Exam. For more information on this policy, see the Graduate School Academic Policies and Procedures website. It is also important to be aware of the Graduate School’s Degree Deadlines when deciding when to defend and deposit your thesis.

We encourage you to review Preparing for Graduation (a list of resources compiled by CMB to help you prepare for your thesis defense and graduation) well in advance of scheduling your thesis defense. If an in person meeting is not possible, please see the CMB Virtual Meeting Guide .

The UW-Madison Committee on the Future of the Dissertation has created some general Proposed Standards for Examining Dissertations , which might be useful to students and their committees as they prepare their dissertations. Be sure to detail your contributions and the contributions of others on the title page of each research chapter in your written dissertation. For example, if data or specific figures were generated by others in your lab, acknowledge this contribution. In addition, if the work in the chapter has been published, provide complete citations including the title, names of all authors, book or journal, volume number, page numbers, and year of publication. You can also find sample dissertations through the Proquest UW-Madison Dissertations & Theses search on the UW-Madison Libraries website (note: we’ve had the best luck finding theses using the searches under the “In Library Databases” section). If a publication or a patent is pending, students may request an embargo on their thesis for up to two years, so it is possible that recent theses will not yet be available.

Requirements to Complete

A few months prior to the thesis defense (“six-month meeting”):.

• A few months prior to the thesis defense, students should have a meeting with their committee to determine whether they are ready to move on to the thesis defense. The committee should agree the student is ready before the thesis defense is scheduled. This meeting usually occurs about six months prior to the defense but the timing can vary significantly. At the six-month meeting, students should present an outline of their thesis to their committee (an outline example can be found on the Progress Report page under Six-Month Meeting). Please fill out and submit a Progress Report Form and check the box indicating that this is your six-month meeting.

Sample Thesis Outline:

I. Chapter 1: Introduction*

II. Chapter 2: Paper 1 (published, submitted, or in preparation)**

III. Chapter 3: Paper 2 (same as Chapter 2)

IV. Chapter 4: Paper 3 (same as Chapter 2)

V. Chapter 5: Future directions

VI. Appendix 1: Unpublished data***

VII. Appendix 2: Unpublished data (same as Appendix 1)

*Provide a chapter title that encapsulates the topic for the entire thesis. The title page for each of the research chapters should indicate your contributions to the work (e.g., Figures 1-5) as well as the contributions of others.

**Provide the paper title. It would also be helpful to include subheadings (A, B, C…) with the major findings of the paper. If the paper has been published, you should provide the citation.

***Provide a title for data that do not fit into one of the paper chapters but constitute an important part of your thesis research.  Each appendix could include single or multiple figures.

CMB’s thesis format is the same as the one outlined by the Graduate School.

At Least Three Weeks Prior to the Thesis Defense:

• Schedule the Thesis Defense with the Thesis Committee
• Send an email notification with the scheduled date, defense location, defense time, thesis title, and listing of thesis committee members to the CMB Office
• The CMB Office will then request a warrant from the Graduate School
• Upon approval, the Graduate School will send an email notification to the student and the CMB Office. The CMB Office will then send additional details to the student
• Read through the Graduate School’s page on “ Guide to Preparing Your Doctoral Dissertation ” and follow the instructions outlined here to submit your Thesis Defense.
• If changes in the Thesis Committee or date of the dissertation are made after a warrant has been requested, please notify the CMB Office immediately so we can request edits to the warrant.

At Least Two Weeks Prior to the Thesis Defense:

• Submit a copy of the Thesis Defense to all members of the Thesis Committee
• The CMB Office will create a Thesis Defense flyer and distribute this announcement to the current CMB faculty trainer and graduate student email list, publish it in the weekly e-newsletter, and post it on the calendar of events on the CMB website
• Notify your departmental payroll coordinator of the date you are expecting to defend and deposit your thesis

At the Thesis Defense:

• All five committee members must be present
• Give a presentation about the research
• Defend and answer questions asked by the Thesis Committee

Following the Thesis Defense:

• Request electronic signatures of all Thesis Committee members on the Thesis Defense warrant through MyGradPortal
• All corrections and revisions of the Thesis Defense must be made before submitting your Thesis Defense
• Submit Thesis Defense electronically

Submit the Following Materials:

• Alumni Information Form
• Thesis Defense warrant via MyGradPortal
• Survey of Earned Doctorates (SED) certificate of completion
• Graduate School Doctoral Exit Survey (DES) certificate of completion

Professionally Print Your Thesis:

If you’d like to turn your thesis into a lasting keepsake, here’s how:

• Navigate to Houchen Bindery , where they specialize in on demand printing.
• Printing Suggestions: Go for a vertical layout, print on both sides, and choose a sleek black binding with either white or gold printing. You will need to note how many pages need color, as it is a factor in the final price.
• Make sure the spine of your printed thesis includes: Last Name, First Name M.I. – Ph.D. – 2024 (Example: Wassarman, David A. – Ph.D. – 2024)
• Expect your professionally printed thesis to be ready in around 2-3 weeks.

Harvard University COVID-19 updates

Undergraduate Molecular and Cellular Biology (MCB) Research and Thesis

Research and thesis.

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Concentrators are encouraged to do research in any area of the life sciences of interest. Many labs on the Cambridge campus as well as labs at Harvard Medical School or affiliated hospitals host undergraduate researchers. Talk to the ADUS, Dominic Mao ,, Concentration Advisor Monique Brewster , or to the Co-Head-Tutors, about how to find a lab that matches your interests. You may also contact the undergraduate science research advisor, Kate Penner

Useful links:

• How to get involved with research.
• Open undergraduate research positions
• URAF Independent Research Fellowships

A senior thesis is a year-long research for credit course worth 8 credits and is letter graded. Students must identify a faculty sponsor that is willing to host and mentor them latest by the end of Spring semester of Junior year (students may decide to start earlier) since the thesis proposal is due mid-July before senior year. Faculty sponsors must be a Harvard faculty or an affiliate of Harvard. Once your proposal is accepted, you may enroll in MCB/CPB 99AB. Students enrolled in 99AB are expected to work ~15 hours/week during term time. Research mentors are expected to provide training on both the science and writing. The undergraduate office will provide supplemental support by hosting a number of workshops and exercises aimed at improving the quality of presentation and writing.

A full list of MCB and CPB undergraduate theses is available here.

General Catalog

Biochemistry and molecular biology, phd.

This is the first version of the 2024–25 General Catalog. Please check back regularly for changes. The final edition and the historical PDF will be published during the fall semester.

The Doctor of Philosophy in biochemistry and molecular biology requires a minimum of 72 s.h. of graduate credit (34 s.h. of coursework and 38 s.h. of research). Students must maintain a cumulative grade-point average of at least 3.00 to earn the degree. Qualified students interested in earning the Doctor of Medicine along with the PhD may apply to the  Medical Scientist Training Program , which offers a combined MD/PhD program.

Students have the opportunity to tailor their curriculum with courses that enhance their educational goals. They take a combination of graduate-level courses that include a first-year laboratory research rotation course, and seminar courses.

The PhD with a major in biochemistry and molecular biology requires the following coursework.

Core Curriculum

Typical curriculum, first year, fall,  first year, spring, second year, fall, second year, spring, examples of elective coursework, additional requirements, laboratory rotations.

Students rotate through at least three different laboratories during their first academic year; they enroll in  BMB:5261 Research Techniques . The laboratory rotations are approximately ten weeks each. At the conclusion of each rotation, a student meets with an advisory committee of three faculty members. A student is required to present the research and training completed during that rotation. The advisory committee writes a short evaluation of the student's performance and assigns a grade for the laboratory work. The evaluation and grade becomes part of the student's departmental record.

Students participate in the formal teaching programs of the department for at least one semester. First-year students as well as students who are within a year of receiving their PhD degree are usually not asked to teach. Teaching may take a variety of forms, including tutoring, leading discussions and laboratory groups, correcting examinations, preparing teaching materials, and lecturing.

Thesis Research Proposal

During the fall semester of the second year, students in collaboration with their thesis advisor prepare a detailed thesis proposal that describes the proposed research to be conducted for the dissertation as part of  BMB:5282 Seminar .

Comprehensive Examination

The comprehensive examination has two parts: a written proposal and an oral defense of the proposal. The examination must be taken before June 30 of the second year.

Written Report of Comprehensive Examination

Students receive their topic by March 1 and their written examination is submitted to their committee by April 22. The written proposal should have a cover page followed by no more than 20 pages. For more information, a detailed guide is located in the Department of Biochemistry and Molecular Biology  Graduate Student Manual .

Oral Presentation of Comprehensive Examination

Questions during the oral examination may come from the examination proposal, the PhD thesis proposal, or other general areas of biochemistry and molecular biology. To pass the oral comprehensive examination, students must perform satisfactorily both in defense of the examination proposal and in answering general biochemistry and molecular biology questions that are germane to the proposal or that are important for a full understanding of the proposed experiments and their interpretation.

The Fifth-Semester Seminar

After successful completion of the comprehensive examination, usually the fall semester of the third year (the fifth semester), students update and revise the written PhD thesis proposal prepared during the fall semester of the second year (prior to the comprehensive examination), and present a seminar on the thesis research to the department at one of the weekly biochemistry and molecular biology workshops.

The Fourth-Year Workshop

In the fourth year, during fall or spring, students are asked to present at one of the weekly departmental workshops. The presentation is based on their research.

The Fifth-Year Retreat

The Department of Biochemistry and Molecular Biology holds a yearly retreat where students and faculty present their current research. Students in their fifth year may be asked to give an oral presentation at the retreat.

Final Examination

The five-member PhD thesis committee serves as an advisory body for preparation of the thesis. This committee meets with students to review the material that is expected to be incorporated in the thesis. Although meetings of the candidates with the committee should be yearly, the candidates, thesis advisor, or the committee can request a meeting at any time. A final draft of the thesis must be given to all members of the committee two weeks before the final examination. The final examination takes the form of a seminar presented to the department. This presentation is announced according to Graduate College policy. Questions, comments, and discussion then follow. After the seminar, candidates meet with their committee for the final thesis defense. The PhD is not awarded until the thesis is signed. In some cases, revisions may be required.

Students may work toward the Doctor of Medicine degree and a PhD in biochemistry and molecular biology in a combined degree program offered by the Department Biochemistry and Molecular Biology and the Carver College of Medicine. Applicants must be admitted to both programs before they may be admitted to the combined degree program. See the  Medical Scientist Training Program  (Carver College of Medicine) in the catalog.

Applicants must have a baccalaureate degree from a regionally accredited U.S. college or university, or an equivalent degree from another country as determined by the Office of Admissions. Those who apply must have an undergraduate grade-point average of at least 3.00. Applicants must meet the admission requirements of the Graduate College; see the  Manual of Rules and Regulations  on the Graduate College website.

Appropriate preparation includes one-year, college-level courses in organic and physical chemistry, biology, physics, and mathematics through calculus. Students are expected to have had one or more introductory courses in biochemistry.

Students admitted to the PhD program in biochemistry and molecular biology routinely receive a stipend and tuition support.

Graduates have secured a variety of career positions, including in academic institutions and the government, and as scientists, physicians, lecturers, and science educators. Some go on to pursue postdoctoral or additional training, and others land jobs in business and industry.

Sample Plan of Study

Sample plans represent one way to complete a program of study. Actual course selection and sequence will vary and should be discussed with an academic advisor. For additional sample plans, see  MyUI .

This sample plan is being reviewed and will be added at a later date.

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The Biochemistry and Molecular Biology doctorate degree program at IU School of Medicine typically takes five years to complete. The program includes two years of course work and three years of conducting original research full-time, culminating in a PhD thesis. This program prepares graduates for careers in academic research and teaching or in the business and industry side of bioscience.

Program Requirements

• A minimum of 30 credit hours of didactic coursework
• A minimum of 45 credit hours of research (B855)
• A minimum of 90 credit hours consisting of required and elective courses
• A scholarly thesis based on approved original research with oversight from a faculty mentor. Potential research topics include structural biology, signal transduction, drug development, regulation of gene expression, cancer biology and metabolic disorders.
• Minimum 3.0 GPA and a B- or better in all PhD coursework

Biochemistry PhD students must take at least two (of the available eight) two-credit Biochemistry core courses (BIOC-B811, GRDM-G805, G807, G817, G848, G852, G825, G826), which are offered among spring electives for the Indiana Biomedical Gateway Program (IBGP) or in the fall. These may be taken in year one or later years.

• GRDM-G715 Biomed I – Biomedical Science I – Biochemical Basis of Biological Processes*: 2 credits
• GRDM-G716 Biomed II – Biomedical Science II – Molecular Biology and Genetics*: 2 credits
• GRDM-G717 Biomed III – Biomedical Science III – Cellular Basis of Systems Biology*: 2 credits
• GRDM-G505 Responsible Conduct of Research*: 1 credit
• GRDM-G700 Translating Foundational Science to Contemporary Knowledge*: 1 credit
• GRDM-G702 Entering Biomedical Science Research*: 1 credit
• GRDM-G718 Research in Biomedical Science (first rotation): 2 credits

Biochemistry Core Electives

• GRDM-G805 Diabetes and Obesity: 2 credits
• GRDM-G825 Advanced Topics in Molecular Biology: 2 credits
• GRDM-G826 Synthetic Biology and Biologics: 2 credits

Research Communication (one of the following)

• COMM-C534 Distilling Your Message*: 1 credit
• COMM-C533 Improvisation for Scientists*: 1 credit
• ENG-W533 Science Writing for Public Readers*: 1 credit
• GRDM-G507 Reagent Validation as a Means for Enhanced Research Reproducibility*: 1 credit
• GRDM-G718 Research in Biomedical Science Rotations 2 and 3: 4 credits
• Indiana Biomedical Gateway Program open enrollment electives
• GRDM-G817 Molecular basis of cell structure and function: 2 credits
• GRDM-G852 Concepts of cancer biology: 2 credits
• GRDM-G807 Structural and chemical biology: 2 credits
• GRDM-G848 Bioinformatics applications to proteomics and genomics: 2 credits

Biochemistry Course Descriptions

Fall- Total of 10 credit hours

• GRDM-G855 Experimental Design and Research Biostatistics*: 1 credit
• BIOC- B890 Seminar: 1 credit
• Major course requirements: variable
• Minor course requirements: variable
• BIOC-B855 Research: variable

Spring- Total of 10 credit hours

• BIOC-B803 Advanced Biochemistry: 1 credit

During years 3 through 5, the student will take didactic courses if needed to fulfill either requirements for the biochemistry major or their chosen minor. In year 3, the student will typically register for a total of 10 cr. hours each fall and spring, including 1 cr. hour of BIOC-B890. 4 credit hours of B890 are required before advancing to candidacy.   The student will advance to candidacy upon completion of both major and minor coursework and having reached a total of 30 or more didactic hours of coursework as well as successful defense of their thesis proposal which serves as the students qualifying examination.

Work will continue in the field of the candidate’s thesis. Emphasis will be on the ability to pursue research with relative independence and responsibility.

MD/PhD students are not required to take GRDM-G715-717 but are expected to perform lab rotations during summer breaks from medical school classes. MD/PhD students must take two or more of the two-credit Biochemistry core courses (GRDM-G805, G807, G817, G852, G825, G826, G848) along with other courses required of IBMG and Biochemistry and Molecular Biology PhD students (GRDM-G505, G655, G855, BIOC-B803 and B890 [4x]).

• Financial Aid  PhD students with eligibility to work in the U.S. receive a competitive annual stipend without a Teaching Assistant requirement ($29,000 for the 2020-2021 academic year) as well as tuition scholarships and health and dental insurance. Find additional financial aid information from the IUPUI Office of Student Financial Services .

Bioscience PhD Programs

Main navigation, molecular biology program.

Exceptionally collaborative and rigorous interdisciplinary graduate training environment designed to equip students with a strong foundation for a career in the modern biomolecular sciences.

• More than 180 labs in eight diverse departments of Biochemistry, Human Genetics, Neurobiology, Oncological Sciences, Pharmacology & Toxicology, the School of Biological Sciences, the  Division of Pathology in the Department of Microbiology and Immunology, and Nutrition and Integrative Physiology
• Students carry out cutting-edge thesis research in world-class facilities
• Curriculum provides a solid background in key areas of modern molecular biology
• Designed to teach independent, critical thinking skills, and grant writing
• Electives give students a wide variety of options in tailoring coursework
• After the first year, students leave the Program and formally join one of the seven science departments above to continue thesis research in the laboratory of a Program faculty member
• Join our research community!

Program Benefits

Students who are accepted into the program enjoy the following benefits:

• Annual fellowship / Living stipend support ( $37,000 for the 2024-2025 academic year )
• $1,000 Starting Allocation
• Tuition Waver
• Health Insurance
• Outstanding Core Facilities
• Collaborative Environment

Interest Groups

One of the strengths of the MB program are the cross-departmental interest groups, which facilitate collaboration and offer opportunities for students to become more engaged in specific research topics. Interest groups comprise communities of University of Utah students and faculty with shared research interests and goals.

Biochemistry / Structural Biology Interest Group

Associated laboratories aim to understand the molecular basis for important biological processes by using a wide variety of experimental approaches, including x-ray crystallography, NMR, electron microscopy, enzymology, virology, and protein design.

Cancer / Cell Biology Interest Group

The genesis of human cancer arises from alterations in fundamental cell biological processes.  The members of the Cancer/Cell Biology Interest Group study normal and cancer cell physiology in order to understand cancer from its beginnings and use that information to create and improve cancer treatments.

Developmental Biology Interest Group

Recent studies in developmental biology have been at the leading edge of modern biological research. Through combined genetic, genomic and molecular approaches, studies of bacteria, yeast, C. elegans, Drosophila, mammals, Xenopus, chick and zebrafish have provided a strong foundation for understanding the common developmental pathways shared by all higher organisms.

Gene Expression Interest Group

Regulation of gene expression is fundamental to cell identity, fate, and growth control. Thus, researchers interested in the regulation of gene expression come from a wide range of biological disciplines including cancer biology, developmental biology, human genetics and cell signaling (among others).

Genetics Interest Group

Associated laboratories utilize the power of genetics to answer important questions in biology, ranging from basic developmental processes to the diagnosis and treatment of genetic disease including understand the underlying pathophysiology of genetic disorders.

Microbiology / Immunology Interest Group

Microbiology and Immunology are disciplines with great impact on human biology and health. Associated laboratories are enhanced by a community approach that promotes interactions between clinician scientists and investigators studying basic questions in immunology and host-pathogen interactions.

Neurobiology Interest Group

The human brain is perhaps the most complex object known—it has more neurons than the Milky Way galaxy has stars. Understanding how nervous systems are put together, work, and change is one of the main challenges facing biologists today. At the University of Utah we are exceptionally strong in using model systems to study neurobiology to increase knowledge of the mechanisms and molecules that underlie the development and function of nervous systems.

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Molecular Biology (M.Sc./Ph.D.)

• Application

The international programme is a joint venture of the University of Göttingen, the University Medical Center, the German Primate Center and the Max Planck Institute for Multidisciplinary Sciences. Leading scientists of all partners offer research-oriented training across a broad spectrum of modern molecular life sciences with access to their state-of-the art laboratories during methods courses and individually supervised research projects.

After one year of intensive course work, students of this integrated Master/PhD programme can continue with a six-month thesis to obtain a Master of Science degree, or join the PhD part directly (fast-track option).

The award-winning program is recognized as an International Max Planck Research School, offering stipends to all of its students. A dedicated team of staff members provides a variety of support, individual counselling and career guidance.

Get to know us

Programme Homepage

Horizons in Molecular Biologye

Incoming Office

Programme description.

The integrated Master/PhD program offers excellent study and research conditions to prepare aspiring young scientists for a professional career in academia or science-related professions in the private or public sector. At any stage of their studies, the students are exposed to a broad spectrum of theoretical and practical training, individual counselling and guidance to prepare for taking well-informed decisions on their next career steps.

Study contents

In 36 lecture weeks (accompanied by tutorials), 20 methods courses during the first three months of the master's program, and three eight-week research projects (lab rotations), students acquire in-depth knowledge in the following subject areas of molecular biosciences:

Molecular Biology, Biochemistry, Structural Biology, Genetics, Bioinformatics, Biophysics, Metabolic Physiology, Cell Biology, Oncology, Immunobiology, Neurobiology, Developmental Biology, Microbiology, Systems Biology, Biotechnology.

Professional skills such as good scientific practice, scientific writing, presentation techniques, design of scientific illustrations, and handling of experimental animals are taught in workshops and applied during lab rotations and reporting seminars.

In addition, all foreign students can participate in free German language courses, which are optionally offered at different levels as introductory intensive courses and evening courses throughout the study program.

During the subsequent PhD studies, students benefit from the wide range of qualifications (scientific methods courses, professional skills workshops, industry excursions, language courses, other events) offered by the Graduate School GAUSS and the Graduate Center GGNB.

Annual retreats of the Molecular Biology program, the student-organized international symposium Horizons in Molecular Biology and regular cultural nights contribute to a close personal and scientific exchange.

Financial support

Master's stipends: All students are supported by a stipend of the Max Planck Society at the amount of 900 €/month during their entire master's studies, unless they receive comparable scholarships from other sources.

PhD funding: During their PhD studies, students are usually funded by the supervising institution through work/funding contracts. The program has as budget available for start-up, bridging, or wrap-up funding as needed.

Student activities: Budgets are available for students to attend international scientific conferences and student-organized, scientific or cultural events.

Counselling and support

Newly admitted students receive info letters in preparation of their studies, advice, and a variety of administrative support as part of a two-week orientation program prior to their first year of study. Throughout their studies, students receive individual counselling from members of the programme regarding their course of study and the advancement of their skills, interests, and personal development.

The Molecular Biology program is regularly evaluated by independent external reviewers and has been rated several times as a model best practice example. Together with the Neurosciences program, it received the 2004 prize for excellent support services for foreign students by the Federal Foreign Minister and was awarded the label "Top 10 International Master's Degree Courses made in Germany" by "Stifterverband für die Deutsche Wissenschaft" together with the German Academic Exchange Service (DAAD) in a national contest.

Yearbook and Newsletter

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Related programmes

• Neurosciences (M.Sc./Ph.D./MD-Ph.D.)
• Molecular Life Sciences: Microbiology, Biotechnology and Biochemistry (M.Sc.)
• Developmental, Neural and Behavioral Biology (M.Sc.)
• Molecular Medicine (M.Sc.)

Intensive course program in the first year

During the first year of research-oriented training, students earn 90 credits (ECTS) in a combination of theoretical and practical modules between October and July:

• Lectures / Tutorials (October-July)
• Methods courses (October-December)
• Short presentations by research groups (October-November)
• Professional skills (November-January)
• Research projects / Lab rotations (January-June)
• Master seminar (March-June)

The first year of study concludes with a written and two oral Master’s examinations in August.

Integrated Master’s/PhD concept

After successful completion of the Master’s examinations, a six-month Master’s thesis (30 credits ECTS) leads to the award of the Master of Science degree concluding the Master’s program after 18 months. The majority of students continues in the Molecular Biology program with their PhD research without the need for panel-based admission interviews.

Alternatively, students who have passed the Master’s examinations at the end of the first year with good or excellent results qualify for direct admission to a three-year doctoral project in one of the participating research groups without being required to complete a Master's thesis first (fast-track option).

Regulations and module directory

• Current und older versions

Previous education

• Bachelor’s degree or equivalent.
• 120 credits (ECTS or equivalent) at the time of application.
• Degree program in molecular biosciences, cell biology, biochemistry, biophysics, biotechnology, or a closely related discipline.

Language requirements

• Very good knowledge of English is required.
• If English is not the native language, the language skills can be proven, for example, by an internationally recognized language test, an English-language bachelor's degree or an extended stay in an English-speaking country.
• Knowledge of the German language is not required.

Three-stage admission process

The online application portal is open for each application round from September 15 to January 15 (start of studies: October).

In addition to the information on personal background and motivation and the upload of all relevant documents, the contact details of two reviewers for letters of recommendation should also be provided. The reviewers should be informed by the students in time about the planned application.

All applicants will be informed in the second half of January whether they have qualified for the second selection round (15-minute online interviews at the end of January).

During a third selection round in mid-February, admission decisions are made on the basis of two personal interviews (30 minutes each).

Academic Advising

Dr. Steffen Burkhardt

Justus-von-Liebig-Weg 11 37077 Göttingen

Phone: +49 551 39-26685/26686 E-Mail: [email protected]

Molecular Biology Office

Kerstin Grüniger

Phone: +49 551 39-26686 E-Mail: [email protected]

Modal title

Address:  2001 New Science Facility Phone:  906-227-2310 Fax:  906-227-1063 Web Address:  https://nmu.edu/biology/ Department Email:  [email protected] Department Code:  1254840944 Type:  Department

Biology at NMU

The Biology Department offers two master of science degrees, Biology and Integrated Biosciences.  Students create individual programs of study that are designed to meet specific needs.

The thesis program in Biology may be broadly based and include course work in several areas of biology, or it may provide for concentration within a specific area of biology.  One of the strengths of these master’s degree programs is that they provide the opportunity for students to participate in an in‐depth graduate research project. Many of these projects utilize field-oriented research because of the unique geographical setting of the university. Laboratory research is also supported in such areas as neurobiology, genetics, virology, immunology and physiology. Department facilities include a green house, aquatics laboratory, herbarium, Lake Superior research boat, the Longyear Forest, confocal microscope, molecular biology labs, a small‐animal colony, and a zoological collection. The members of the Biology Department faculty all have doctoral degrees, and most are actively involved in the department’s graduate program. Thesis advisors for students in this program are drawn from the Biology Department faculty who maintain graduate faculty Level 3 status.

Biology Program Mission

The graduate program in Biology provides quality graduate education through student-faculty collaboration in biological research and scholarly activity. Graduates will have a strong understanding of biology and the principles that guide the design and execution of high-quality research. Graduates will have substantial research experience and will be able to successfully communicate scientific information to the public and the scientific community.

Integrated Biosciences

The Master of Science degree program in Integrated Biosciences is designed to meet the needs of students with an interests in the intersection of biology and the other sciences, technology and engineering fields such as chemistry, physics, geology, atmospherics, climatology, mathematics and computer science. Student thesis projects in this program emphasize the interdisciplinary nature of natural sciences and biology, drawing on diverse knowledge bases and skill sets. Projects are developed at the discretion of the student and the student’s interdisciplinary graduate advisory committee. The advisory committee will review the student’s background and determine what training and/or coursework will be required.

Integrated Biosciences Mission

The program in Integrated Biosciences provides quality graduate education through student‐faculty collaboration in interdisciplinary natural science research and scholarly activity with links to biology. Graduates will have a strong understanding of interdisciplinary natural sciences and the principles that guide the design and execution of high‐quality research. Graduates will have substantial research experience and will be able to successfully communicate scientific information to the public and the scientific community.

Synthetic DNA nanopores for direct molecular transmission between lipid vesicles †

* Corresponding authors

a School of Engineering, Tokyo Institute of Technology, 403, Ishikawadai Bldg. 3, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan E-mail: [email protected]

b Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8563, Japan

c Department of Maternal-Fetal Biology, National Center for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan

Lipid vesicles hold potential as artificial cells in bottom-up synthetic biology, and as tools in drug delivery and biosensing. Transmitting molecular signals is a key function for vesicle-based systems. One strategy to achieve this function is by releasing molecular signals from vesicles through nanopores. Nevertheless, in this strategy, an excess of molecular signals may be required to reach the targets, due to the dispersion of the signals during diffusion. The key to achieving the efficient utilization of signals is to shorten the distance between the sender vesicle and the target. Here, we present a pair of DNA nanopores that can connect and form a direct molecular pathway between vesicles. The nanopores are self-assembled from nine single DNA strands, including six 14-nucleotide single-stranded overhangs as sticky-end segments, enabling them to bind with each other. Incorporating nanopores shortens the distance between different populations of vesicles, allowing less diffusion of molecules into bulk solution. To further reduce the loss of molecules, a DNA nanocap is added to one of the nanopore's openings. The nanocap can be removed through the toehold-mediated DNA strand displacement when the nanopore meets its counterpart. Our DNA nanopores provide a novel molecular transmission tool to lipid vesicles-based systems.

Supplementary files

• Supplementary information PDF (6260K)

Article information

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Synthetic DNA nanopores for direct molecular transmission between lipid vesicles

Z. Peng, S. Kanno, K. Shimba, Y. Miyamoto and T. Yagi, Nanoscale , 2024, Advance Article , DOI: 10.1039/D4NR01344A

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