hypothesis oriented research

What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

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Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

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This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism, run a free check.

Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

• Manuscript Preparation

What is and How to Write a Good Hypothesis in Research?

• 4 minute read
• 319.9K views

Table of Contents

One of the most important aspects of conducting research is constructing a strong hypothesis. But what makes a hypothesis in research effective? In this article, we’ll look at the difference between a hypothesis and a research question, as well as the elements of a good hypothesis in research. We’ll also include some examples of effective hypotheses, and what pitfalls to avoid.

What is a Hypothesis in Research?

Simply put, a hypothesis is a research question that also includes the predicted or expected result of the research. Without a hypothesis, there can be no basis for a scientific or research experiment. As such, it is critical that you carefully construct your hypothesis by being deliberate and thorough, even before you set pen to paper. Unless your hypothesis is clearly and carefully constructed, any flaw can have an adverse, and even grave, effect on the quality of your experiment and its subsequent results.

Research Question vs Hypothesis

It’s easy to confuse research questions with hypotheses, and vice versa. While they’re both critical to the Scientific Method, they have very specific differences. Primarily, a research question, just like a hypothesis, is focused and concise. But a hypothesis includes a prediction based on the proposed research, and is designed to forecast the relationship of and between two (or more) variables. Research questions are open-ended, and invite debate and discussion, while hypotheses are closed, e.g. “The relationship between A and B will be C.”

A hypothesis is generally used if your research topic is fairly well established, and you are relatively certain about the relationship between the variables that will be presented in your research. Since a hypothesis is ideally suited for experimental studies, it will, by its very existence, affect the design of your experiment. The research question is typically used for new topics that have not yet been researched extensively. Here, the relationship between different variables is less known. There is no prediction made, but there may be variables explored. The research question can be casual in nature, simply trying to understand if a relationship even exists, descriptive or comparative.

How to Write Hypothesis in Research

Writing an effective hypothesis starts before you even begin to type. Like any task, preparation is key, so you start first by conducting research yourself, and reading all you can about the topic that you plan to research. From there, you’ll gain the knowledge you need to understand where your focus within the topic will lie.

Remember that a hypothesis is a prediction of the relationship that exists between two or more variables. Your job is to write a hypothesis, and design the research, to “prove” whether or not your prediction is correct. A common pitfall is to use judgments that are subjective and inappropriate for the construction of a hypothesis. It’s important to keep the focus and language of your hypothesis objective.

An effective hypothesis in research is clearly and concisely written, and any terms or definitions clarified and defined. Specific language must also be used to avoid any generalities or assumptions.

Use the following points as a checklist to evaluate the effectiveness of your research hypothesis:

• Predicts the relationship and outcome
• Simple and concise – avoid wordiness
• Clear with no ambiguity or assumptions about the readers’ knowledge
• Observable and testable results
• Relevant and specific to the research question or problem

Research Hypothesis Example

Perhaps the best way to evaluate whether or not your hypothesis is effective is to compare it to those of your colleagues in the field. There is no need to reinvent the wheel when it comes to writing a powerful research hypothesis. As you’re reading and preparing your hypothesis, you’ll also read other hypotheses. These can help guide you on what works, and what doesn’t, when it comes to writing a strong research hypothesis.

Here are a few generic examples to get you started.

Eating an apple each day, after the age of 60, will result in a reduction of frequency of physician visits.

Budget airlines are more likely to receive more customer complaints. A budget airline is defined as an airline that offers lower fares and fewer amenities than a traditional full-service airline. (Note that the term “budget airline” is included in the hypothesis.

Workplaces that offer flexible working hours report higher levels of employee job satisfaction than workplaces with fixed hours.

Each of the above examples are specific, observable and measurable, and the statement of prediction can be verified or shown to be false by utilizing standard experimental practices. It should be noted, however, that often your hypothesis will change as your research progresses.

Language Editing Plus

Elsevier’s Language Editing Plus service can help ensure that your research hypothesis is well-designed, and articulates your research and conclusions. Our most comprehensive editing package, you can count on a thorough language review by native-English speakers who are PhDs or PhD candidates. We’ll check for effective logic and flow of your manuscript, as well as document formatting for your chosen journal, reference checks, and much more.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

About the author

Muhammad Hassan

Researcher, Academic Writer, Web developer

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Research Hypothesis: What It Is, Types + How to Develop?

A research study starts with a question. Researchers worldwide ask questions and create research hypotheses. The effectiveness of research relies on developing a good research hypothesis. Examples of research hypotheses can guide researchers in writing effective ones.

In this blog, we’ll learn what a research hypothesis is, why it’s important in research, and the different types used in science. We’ll also guide you through creating your research hypothesis and discussing ways to test and evaluate it.

What is a Research Hypothesis?

A hypothesis is like a guess or idea that you suggest to check if it’s true. A research hypothesis is a statement that brings up a question and predicts what might happen.

It’s really important in the scientific method and is used in experiments to figure things out. Essentially, it’s an educated guess about how things are connected in the research.

A research hypothesis usually includes pointing out the independent variable (the thing they’re changing or studying) and the dependent variable (the result they’re measuring or watching). It helps plan how to gather and analyze data to see if there’s evidence to support or deny the expected connection between these variables.

Importance of Hypothesis in Research

Hypotheses are really important in research. They help design studies, allow for practical testing, and add to our scientific knowledge. Their main role is to organize research projects, making them purposeful, focused, and valuable to the scientific community. Let’s look at some key reasons why they matter:

• A research hypothesis helps test theories.

A hypothesis plays a pivotal role in the scientific method by providing a basis for testing existing theories. For example, a hypothesis might test the predictive power of a psychological theory on human behavior.

• It serves as a great platform for investigation activities.

It serves as a launching pad for investigation activities, which offers researchers a clear starting point. A research hypothesis can explore the relationship between exercise and stress reduction.

• Hypothesis guides the research work or study.

A well-formulated hypothesis guides the entire research process. It ensures that the study remains focused and purposeful. For instance, a hypothesis about the impact of social media on interpersonal relationships provides clear guidance for a study.

• Hypothesis sometimes suggests theories.

In some cases, a hypothesis can suggest new theories or modifications to existing ones. For example, a hypothesis testing the effectiveness of a new drug might prompt a reconsideration of current medical theories.

• It helps in knowing the data needs.

A hypothesis clarifies the data requirements for a study, ensuring that researchers collect the necessary information—a hypothesis guiding the collection of demographic data to analyze the influence of age on a particular phenomenon.

• The hypothesis explains social phenomena.

Hypotheses are instrumental in explaining complex social phenomena. For instance, a hypothesis might explore the relationship between economic factors and crime rates in a given community.

• Hypothesis provides a relationship between phenomena for empirical Testing.

Hypotheses establish clear relationships between phenomena, paving the way for empirical testing. An example could be a hypothesis exploring the correlation between sleep patterns and academic performance.

• It helps in knowing the most suitable analysis technique.

A hypothesis guides researchers in selecting the most appropriate analysis techniques for their data. For example, a hypothesis focusing on the effectiveness of a teaching method may lead to the choice of statistical analyses best suited for educational research.

Characteristics of a Good Research Hypothesis

A hypothesis is a specific idea that you can test in a study. It often comes from looking at past research and theories. A good hypothesis usually starts with a research question that you can explore through background research. For it to be effective, consider these key characteristics:

• Clear and Focused Language: A good hypothesis uses clear and focused language to avoid confusion and ensure everyone understands it.
• Related to the Research Topic: The hypothesis should directly relate to the research topic, acting as a bridge between the specific question and the broader study.
• Testable: An effective hypothesis can be tested, meaning its prediction can be checked with real data to support or challenge the proposed relationship.
• Potential for Exploration: A good hypothesis often comes from a research question that invites further exploration. Doing background research helps find gaps and potential areas to investigate.
• Includes Variables: The hypothesis should clearly state both the independent and dependent variables, specifying the factors being studied and the expected outcomes.
• Ethical Considerations: Check if variables can be manipulated without breaking ethical standards. It’s crucial to maintain ethical research practices.
• Predicts Outcomes: The hypothesis should predict the expected relationship and outcome, acting as a roadmap for the study and guiding data collection and analysis.
• Simple and Concise: A good hypothesis avoids unnecessary complexity and is simple and concise, expressing the essence of the proposed relationship clearly.
• Clear and Assumption-Free: The hypothesis should be clear and free from assumptions about the reader’s prior knowledge, ensuring universal understanding.
• Observable and Testable Results: A strong hypothesis implies research that produces observable and testable results, making sure the study’s outcomes can be effectively measured and analyzed.

When you use these characteristics as a checklist, it can help you create a good research hypothesis. It’ll guide improving and strengthening the hypothesis, identifying any weaknesses, and making necessary changes. Crafting a hypothesis with these features helps you conduct a thorough and insightful research study.

Types of Research Hypotheses

The research hypothesis comes in various types, each serving a specific purpose in guiding the scientific investigation. Knowing the differences will make it easier for you to create your own hypothesis. Here’s an overview of the common types:

01. Null Hypothesis

The null hypothesis states that there is no connection between two considered variables or that two groups are unrelated. As discussed earlier, a hypothesis is an unproven assumption lacking sufficient supporting data. It serves as the statement researchers aim to disprove. It is testable, verifiable, and can be rejected.

For example, if you’re studying the relationship between Project A and Project B, assuming both projects are of equal standard is your null hypothesis. It needs to be specific for your study.

02. Alternative Hypothesis

The alternative hypothesis is basically another option to the null hypothesis. It involves looking for a significant change or alternative that could lead you to reject the null hypothesis. It’s a different idea compared to the null hypothesis.

When you create a null hypothesis, you’re making an educated guess about whether something is true or if there’s a connection between that thing and another variable. If the null view suggests something is correct, the alternative hypothesis says it’s incorrect. 

For instance, if your null hypothesis is “I’m going to be $1000 richer,” the alternative hypothesis would be “I’m not going to get $1000 or be richer.”

03. Directional Hypothesis

The directional hypothesis predicts the direction of the relationship between independent and dependent variables. They specify whether the effect will be positive or negative.

If you increase your study hours, you will experience a positive association with your exam scores. This hypothesis suggests that as you increase the independent variable (study hours), there will also be an increase in the dependent variable (exam scores).

04. Non-directional Hypothesis

The non-directional hypothesis predicts the existence of a relationship between variables but does not specify the direction of the effect. It suggests that there will be a significant difference or relationship, but it does not predict the nature of that difference.

For example, you will find no notable difference in test scores between students who receive the educational intervention and those who do not. However, once you compare the test scores of the two groups, you will notice an important difference.

05. Simple Hypothesis

A simple hypothesis predicts a relationship between one dependent variable and one independent variable without specifying the nature of that relationship. It’s simple and usually used when we don’t know much about how the two things are connected.

For example, if you adopt effective study habits, you will achieve higher exam scores than those with poor study habits.

06. Complex Hypothesis

A complex hypothesis is an idea that specifies a relationship between multiple independent and dependent variables. It is a more detailed idea than a simple hypothesis.

While a simple view suggests a straightforward cause-and-effect relationship between two things, a complex hypothesis involves many factors and how they’re connected to each other.

For example, when you increase your study time, you tend to achieve higher exam scores. The connection between your study time and exam performance is affected by various factors, including the quality of your sleep, your motivation levels, and the effectiveness of your study techniques.

If you sleep well, stay highly motivated, and use effective study strategies, you may observe a more robust positive correlation between the time you spend studying and your exam scores, unlike those who may lack these factors.

07. Associative Hypothesis

An associative hypothesis proposes a connection between two things without saying that one causes the other. Basically, it suggests that when one thing changes, the other changes too, but it doesn’t claim that one thing is causing the change in the other.

For example, you will likely notice higher exam scores when you increase your study time. You can recognize an association between your study time and exam scores in this scenario.

Your hypothesis acknowledges a relationship between the two variables—your study time and exam scores—without asserting that increased study time directly causes higher exam scores. You need to consider that other factors, like motivation or learning style, could affect the observed association.

08. Causal Hypothesis

A causal hypothesis proposes a cause-and-effect relationship between two variables. It suggests that changes in one variable directly cause changes in another variable.

For example, when you increase your study time, you experience higher exam scores. This hypothesis suggests a direct cause-and-effect relationship, indicating that the more time you spend studying, the higher your exam scores. It assumes that changes in your study time directly influence changes in your exam performance.

09. Empirical Hypothesis

An empirical hypothesis is a statement based on things we can see and measure. It comes from direct observation or experiments and can be tested with real-world evidence. If an experiment proves a theory, it supports the idea and shows it’s not just a guess. This makes the statement more reliable than a wild guess.

For example, if you increase the dosage of a certain medication, you might observe a quicker recovery time for patients. Imagine you’re in charge of a clinical trial. In this trial, patients are given varying dosages of the medication, and you measure and compare their recovery times. This allows you to directly see the effects of different dosages on how fast patients recover.

This way, you can create a research hypothesis: “Increasing the dosage of a certain medication will lead to a faster recovery time for patients.”

10. Statistical Hypothesis

A statistical hypothesis is a statement or assumption about a population parameter that is the subject of an investigation. It serves as the basis for statistical analysis and testing. It is often tested using statistical methods to draw inferences about the larger population.

In a hypothesis test, statistical evidence is collected to either reject the null hypothesis in favor of the alternative hypothesis or fail to reject the null hypothesis due to insufficient evidence.

For example, let’s say you’re testing a new medicine. Your hypothesis could be that the medicine doesn’t really help patients get better. So, you collect data and use statistics to see if your guess is right or if the medicine actually makes a difference.

If the data strongly shows that the medicine does help, you say your guess was wrong, and the medicine does make a difference. But if the proof isn’t strong enough, you can stick with your original guess because you didn’t get enough evidence to change your mind.

How to Develop a Research Hypotheses?

Step 1: identify your research problem or topic..

Define the area of interest or the problem you want to investigate. Make sure it’s clear and well-defined.

Start by asking a question about your chosen topic. Consider the limitations of your research and create a straightforward problem related to your topic. Once you’ve done that, you can develop and test a hypothesis with evidence.

Step 2: Conduct a literature review

Review existing literature related to your research problem. This will help you understand the current state of knowledge in the field, identify gaps, and build a foundation for your hypothesis. Consider the following questions:

• What existing research has been conducted on your chosen topic?
• Are there any gaps or unanswered questions in the current literature?
• How will the existing literature contribute to the foundation of your research?

Step 3: Formulate your research question

Based on your literature review, create a specific and concise research question that addresses your identified problem. Your research question should be clear, focused, and relevant to your field of study.

Step 4: Identify variables

Determine the key variables involved in your research question. Variables are the factors or phenomena that you will study and manipulate to test your hypothesis.

• Independent Variable: The variable you manipulate or control.
• Dependent Variable: The variable you measure to observe the effect of the independent variable.

Step 5: State the Null hypothesis

The null hypothesis is a statement that there is no significant difference or effect. It serves as a baseline for comparison with the alternative hypothesis.

Step 6: Select appropriate methods for testing the hypothesis

Choose research methods that align with your study objectives, such as experiments, surveys, or observational studies. The selected methods enable you to test your research hypothesis effectively.

Creating a research hypothesis usually takes more than one try. Expect to make changes as you collect data. It’s normal to test and say no to a few hypotheses before you find the right answer to your research question.

Testing and Evaluating Hypotheses

Testing hypotheses is a really important part of research. It’s like the practical side of things. Here, real-world evidence will help you determine how different things are connected. Let’s explore the main steps in hypothesis testing:

• State your research hypothesis.

Before testing, clearly articulate your research hypothesis. This involves framing both a null hypothesis, suggesting no significant effect or relationship, and an alternative hypothesis, proposing the expected outcome.

• Collect data strategically.

Plan how you will gather information in a way that fits your study. Make sure your data collection method matches the things you’re studying.

Whether through surveys, observations, or experiments, this step demands precision and adherence to the established methodology. The quality of data collected directly influences the credibility of study outcomes.

• Perform an appropriate statistical test.

Choose a statistical test that aligns with the nature of your data and the hypotheses being tested. Whether it’s a t-test, chi-square test, ANOVA, or regression analysis, selecting the right statistical tool is paramount for accurate and reliable results.

• Decide if your idea was right or wrong.

Following the statistical analysis, evaluate the results in the context of your null hypothesis. You need to decide if you should reject your null hypothesis or not.

• Share what you found.

When discussing what you found in your research, be clear and organized. Say whether your idea was supported or not, and talk about what your results mean. Also, mention any limits to your study and suggest ideas for future research.

The Role of QuestionPro to Develop a Good Research Hypothesis

QuestionPro is a survey and research platform that provides tools for creating, distributing, and analyzing surveys. It plays a crucial role in the research process, especially when you’re in the initial stages of hypothesis development. Here’s how QuestionPro can help you to develop a good research hypothesis:

• Survey design and data collection: You can use the platform to create targeted questions that help you gather relevant data.
• Exploratory research: Through surveys and feedback mechanisms on QuestionPro, you can conduct exploratory research to understand the landscape of a particular subject.
• Literature review and background research: QuestionPro surveys can collect sample population opinions, experiences, and preferences. This data and a thorough literature evaluation can help you generate a well-grounded hypothesis by improving your research knowledge.
• Identifying variables: Using targeted survey questions, you can identify relevant variables related to their research topic.
• Testing assumptions: You can use surveys to informally test certain assumptions or hypotheses before formalizing a research hypothesis.
• Data analysis tools: QuestionPro provides tools for analyzing survey data. You can use these tools to identify the collected data’s patterns, correlations, or trends.
• Refining your hypotheses: As you collect data through QuestionPro, you can adjust your hypotheses based on the real-world responses you receive.

A research hypothesis is like a guide for researchers in science. It’s a well-thought-out idea that has been thoroughly tested. This idea is crucial as researchers can explore different fields, such as medicine, social sciences, and natural sciences. The research hypothesis links theories to real-world evidence and gives researchers a clear path to explore and make discoveries.

QuestionPro Research Suite is a helpful tool for researchers. It makes creating surveys, collecting data, and analyzing information easily. It supports all kinds of research, from exploring new ideas to forming hypotheses. With a focus on using data, it helps researchers do their best work.

Are you interested in learning more about QuestionPro Research Suite? Take advantage of QuestionPro’s free trial to get an initial look at its capabilities and realize the full potential of your research efforts.

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How to Write a Research Hypothesis

• Research Process
• Peer Review

Since grade school, we've all been familiar with hypotheses. The hypothesis is an essential step of the scientific method. But what makes an effective research hypothesis, how do you create one, and what types of hypotheses are there? We answer these questions and more.

Updated on April 27, 2022

What is a research hypothesis?

General hypothesis.

Since grade school, we've all been familiar with the term “hypothesis.” A hypothesis is a fact-based guess or prediction that has not been proven. It is an essential step of the scientific method. The hypothesis of a study is a drive for experimentation to either prove the hypothesis or dispute it.

Research Hypothesis

A research hypothesis is more specific than a general hypothesis. It is an educated, expected prediction of the outcome of a study that is testable.

What makes an effective research hypothesis?

A good research hypothesis is a clear statement of the relationship between a dependent variable(s) and independent variable(s) relevant to the study that can be disproven.

Research hypothesis checklist

Once you've written a possible hypothesis, make sure it checks the following boxes:

• It must be testable: You need a means to prove your hypothesis. If you can't test it, it's not a hypothesis.
• It must include a dependent and independent variable: At least one independent variable ( cause ) and one dependent variable ( effect ) must be included.
• The language must be easy to understand: Be as clear and concise as possible. Nothing should be left to interpretation.
• It must be relevant to your research topic: You probably shouldn't be talking about cats and dogs if your research topic is outer space. Stay relevant to your topic.

How to create an effective research hypothesis

Pose it as a question first.

Start your research hypothesis from a journalistic approach. Ask one of the five W's: Who, what, when, where, or why.

A possible initial question could be: Why is the sky blue?

Do the preliminary research

Once you have a question in mind, read research around your topic. Collect research from academic journals.

If you're looking for information about the sky and why it is blue, research information about the atmosphere, weather, space, the sun, etc.

Write a draft hypothesis

Once you're comfortable with your subject and have preliminary knowledge, create a working hypothesis. Don't stress much over this. Your first hypothesis is not permanent. Look at it as a draft.

Your first draft of a hypothesis could be: Certain molecules in the Earth's atmosphere are responsive to the sky being the color blue.

Make your working draft perfect

Take your working hypothesis and make it perfect. Narrow it down to include only the information listed in the “Research hypothesis checklist” above.

Now that you've written your working hypothesis, narrow it down. Your new hypothesis could be: Light from the sun hitting oxygen molecules in the sky makes the color of the sky appear blue.

Write a null hypothesis

Your null hypothesis should be the opposite of your research hypothesis. It should be able to be disproven by your research.

In this example, your null hypothesis would be: Light from the sun hitting oxygen molecules in the sky does not make the color of the sky appear blue.

Why is it important to have a clear, testable hypothesis?

One of the main reasons a manuscript can be rejected from a journal is because of a weak hypothesis. “Poor hypothesis, study design, methodology, and improper use of statistics are other reasons for rejection of a manuscript,” says Dr. Ish Kumar Dhammi and Dr. Rehan-Ul-Haq in Indian Journal of Orthopaedics.

According to Dr. James M. Provenzale in American Journal of Roentgenology , “The clear declaration of a research question (or hypothesis) in the Introduction is critical for reviewers to understand the intent of the research study. It is best to clearly state the study goal in plain language (for example, “We set out to determine whether condition x produces condition y.”) An insufficient problem statement is one of the more common reasons for manuscript rejection.”

Characteristics that make a hypothesis weak include:

• Unclear variables
• Unoriginality
• Too general
• Too specific

A weak hypothesis leads to weak research and methods . The goal of a paper is to prove or disprove a hypothesis - or to prove or disprove a null hypothesis. If the hypothesis is not a dependent variable of what is being studied, the paper's methods should come into question.

A strong hypothesis is essential to the scientific method. A hypothesis states an assumed relationship between at least two variables and the experiment then proves or disproves that relationship with statistical significance. Without a proven and reproducible relationship, the paper feeds into the reproducibility crisis. Learn more about writing for reproducibility .

In a study published in The Journal of Obstetrics and Gynecology of India by Dr. Suvarna Satish Khadilkar, she reviewed 400 rejected manuscripts to see why they were rejected. Her studies revealed that poor methodology was a top reason for the submission having a final disposition of rejection.

Aside from publication chances, Dr. Gareth Dyke believes a clear hypothesis helps efficiency.

“Developing a clear and testable hypothesis for your research project means that you will not waste time, energy, and money with your work,” said Dyke. “Refining a hypothesis that is both meaningful, interesting, attainable, and testable is the goal of all effective research.”

Types of research hypotheses

There can be overlap in these types of hypotheses.

Simple hypothesis

A simple hypothesis is a hypothesis at its most basic form. It shows the relationship of one independent and one independent variable.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable).

Complex hypothesis

A complex hypothesis shows the relationship of two or more independent and dependent variables.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable) and heart disease (dependent variable).

Directional hypothesis

A directional hypothesis guesses which way the results of an experiment will go. It uses words like increase, decrease, higher, lower, positive, negative, more, or less. It is also frequently used in statistics.

Example: Humans exposed to radiation have a higher risk of cancer than humans not exposed to radiation.

Non-directional hypothesis

A non-directional hypothesis says there will be an effect on the dependent variable, but it does not say which direction.

Associative hypothesis

An associative hypothesis says that when one variable changes, so does the other variable.

Alternative hypothesis

An alternative hypothesis states that the variables have a relationship.

• The opposite of a null hypothesis

Example: An apple a day keeps the doctor away.

Null hypothesis

A null hypothesis states that there is no relationship between the two variables. It is posed as the opposite of what the alternative hypothesis states.

Researchers use a null hypothesis to work to be able to reject it. A null hypothesis:

• Can never be proven
• Can only be rejected
• Is the opposite of an alternative hypothesis

Example: An apple a day does not keep the doctor away.

Logical hypothesis

A logical hypothesis is a suggested explanation while using limited evidence.

Example: Bats can navigate in the dark better than tigers.

In this hypothesis, the researcher knows that tigers cannot see in the dark, and bats mostly live in darkness.

Empirical hypothesis

An empirical hypothesis is also called a “working hypothesis.” It uses the trial and error method and changes around the independent variables.

• An apple a day keeps the doctor away.
• Two apples a day keep the doctor away.
• Three apples a day keep the doctor away.

In this case, the research changes the hypothesis as the researcher learns more about his/her research.

Statistical hypothesis

A statistical hypothesis is a look of a part of a population or statistical model. This type of hypothesis is especially useful if you are making a statement about a large population. Instead of having to test the entire population of Illinois, you could just use a smaller sample of people who live there.

Example: 70% of people who live in Illinois are iron deficient.

Causal hypothesis

A causal hypothesis states that the independent variable will have an effect on the dependent variable.

Example: Using tobacco products causes cancer.

Final thoughts

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How to Develop a Good Research Hypothesis

The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.

This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis

Table of Contents

What is Hypothesis?

Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study.  Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).

What is a Research Hypothesis?

Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Characteristics of a Good Research Hypothesis

As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.

A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.

To help you formulate a promising research hypothesis, you should ask yourself the following questions:

• Is the language clear and focused?
• What is the relationship between your hypothesis and your research topic?
• Is your hypothesis testable? If yes, then how?
• What are the possible explanations that you might want to explore?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate your variables without hampering the ethical standards?
• Does your research predict the relationship and outcome?
• Is your research simple and concise (avoids wordiness)?
• Is it clear with no ambiguity or assumptions about the readers’ knowledge
• Is your research observable and testable results?
• Is it relevant and specific to the research question or problem?

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.

Source: Educational Hub

How to formulate a research hypothesis.

A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.

1. State the problem that you are trying to solve.

Make sure that the hypothesis clearly defines the topic and the focus of the experiment.

2. Try to write the hypothesis as an if-then statement.

Follow this template: If a specific action is taken, then a certain outcome is expected.

3. Define the variables

Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.

Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.

4. Scrutinize the hypothesis

Evaluate assumptions, predictions, and evidence rigorously to refine your understanding.

Types of Research Hypothesis

The types of research hypothesis are stated below:

1. Simple Hypothesis

It predicts the relationship between a single dependent variable and a single independent variable.

2. Complex Hypothesis

It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis

It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.

4. Non-directional Hypothesis

It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.

5. Associative and Causal Hypothesis

The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.

6. Null Hypothesis

Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.

7. Alternative Hypothesis

It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Research Hypothesis Examples of Independent and Dependent Variables

Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).

Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)

You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.

More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.

Importance of a Testable Hypothesis

To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:

• There must be a possibility to prove that the hypothesis is true.
• There must be a possibility to prove that the hypothesis is false.
• The results of the hypothesis must be reproducible.

Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.

What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Frequently Asked Questions

The steps to write a research hypothesis are: 1. Stating the problem: Ensure that the hypothesis defines the research problem 2. Writing a hypothesis as an 'if-then' statement: Include the action and the expected outcome of your study by following a ‘if-then’ structure. 3. Defining the variables: Define the variables as Dependent or Independent based on their dependency to other factors. 4. Scrutinizing the hypothesis: Identify the type of your hypothesis

Hypothesis testing is a statistical tool which is used to make inferences about a population data to draw conclusions for a particular hypothesis.

Hypothesis in statistics is a formal statement about the nature of a population within a structured framework of a statistical model. It is used to test an existing hypothesis by studying a population.

Research hypothesis is a statement that introduces a research question and proposes an expected result. It forms the basis of scientific experiments.

The different types of hypothesis in research are: • Null hypothesis: Null hypothesis is a negative statement to support the researcher’s findings that there is no relationship between two variables. • Alternate hypothesis: Alternate hypothesis predicts the relationship between the two variables of the study. • Directional hypothesis: Directional hypothesis specifies the expected direction to be followed to determine the relationship between variables. • Non-directional hypothesis: Non-directional hypothesis does not predict the exact direction or nature of the relationship between the two variables. • Simple hypothesis: Simple hypothesis predicts the relationship between a single dependent variable and a single independent variable. • Complex hypothesis: Complex hypothesis predicts the relationship between two or more independent and dependent variables. • Associative and casual hypothesis: Associative and casual hypothesis predicts the relationship between two or more independent and dependent variables. • Empirical hypothesis: Empirical hypothesis can be tested via experiments and observation. • Statistical hypothesis: A statistical hypothesis utilizes statistical models to draw conclusions about broader populations.

Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.

Thanks a lot for your valuable guidance.

I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.

Useful piece!

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It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market

Nicely explained

It is really a useful for me Kindly give some examples of hypothesis

It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated

clear and concise. thanks.

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How to Write a Research Hypothesis: Good & Bad Examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Bad hypothesis examples, tips for writing a research hypothesis.

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

Perfect Your Manuscript With Professional Editing

Now that you know how to write a strong research hypothesis for your research paper, you might be interested in our free AI proofreader , Wordvice AI, which finds and fixes errors in grammar, punctuation, and word choice in academic texts. Or if you are interested in human proofreading , check out our English editing services , including research paper editing and manuscript editing .

On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

On This Page:

A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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Research Questions & Hypotheses

Generally, in quantitative studies, reviewers expect hypotheses rather than research questions. However, both research questions and hypotheses serve different purposes and can be beneficial when used together.

Research Questions

Clarify the research’s aim (farrugia et al., 2010).

• Research often begins with an interest in a topic, but a deep understanding of the subject is crucial to formulate an appropriate research question.
• Descriptive: “What factors most influence the academic achievement of senior high school students?”
• Comparative: “What is the performance difference between teaching methods A and B?”
• Relationship-based: “What is the relationship between self-efficacy and academic achievement?”
• Increasing knowledge about a subject can be achieved through systematic literature reviews, in-depth interviews with patients (and proxies), focus groups, and consultations with field experts.
• Some funding bodies, like the Canadian Institute for Health Research, recommend conducting a systematic review or a pilot study before seeking grants for full trials.
• The presence of multiple research questions in a study can complicate the design, statistical analysis, and feasibility.
• It’s advisable to focus on a single primary research question for the study.
• The primary question, clearly stated at the end of a grant proposal’s introduction, usually specifies the study population, intervention, and other relevant factors.
• The FINER criteria underscore aspects that can enhance the chances of a successful research project, including specifying the population of interest, aligning with scientific and public interest, clinical relevance, and contribution to the field, while complying with ethical and national research standards.
• The P ICOT approach is crucial in developing the study’s framework and protocol, influencing inclusion and exclusion criteria and identifying patient groups for inclusion.
• Defining the specific population, intervention, comparator, and outcome helps in selecting the right outcome measurement tool.
• The more precise the population definition and stricter the inclusion and exclusion criteria, the more significant the impact on the interpretation, applicability, and generalizability of the research findings.
• A restricted study population enhances internal validity but may limit the study’s external validity and generalizability to clinical practice.
• A broadly defined study population may better reflect clinical practice but could increase bias and reduce internal validity.
• An inadequately formulated research question can negatively impact study design, potentially leading to ineffective outcomes and affecting publication prospects.

Checklist: Good research questions for social science projects (Panke, 2018)

Research Hypotheses

Present the researcher’s predictions based on specific statements.

• These statements define the research problem or issue and indicate the direction of the researcher’s predictions.
• Formulating the research question and hypothesis from existing data (e.g., a database) can lead to multiple statistical comparisons and potentially spurious findings due to chance.
• The research or clinical hypothesis, derived from the research question, shapes the study’s key elements: sampling strategy, intervention, comparison, and outcome variables.
• Hypotheses can express a single outcome or multiple outcomes.
• After statistical testing, the null hypothesis is either rejected or not rejected based on whether the study’s findings are statistically significant.
• Hypothesis testing helps determine if observed findings are due to true differences and not chance.
• Hypotheses can be 1-sided (specific direction of difference) or 2-sided (presence of a difference without specifying direction).
• 2-sided hypotheses are generally preferred unless there’s a strong justification for a 1-sided hypothesis.
• A solid research hypothesis, informed by a good research question, influences the research design and paves the way for defining clear research objectives.

Types of Research Hypothesis

• In a Y-centered research design, the focus is on the dependent variable (DV) which is specified in the research question. Theories are then used to identify independent variables (IV) and explain their causal relationship with the DV.
• Example: “An increase in teacher-led instructional time (IV) is likely to improve student reading comprehension scores (DV), because extensive guided practice under expert supervision enhances learning retention and skill mastery.”
• Hypothesis Explanation: The dependent variable (student reading comprehension scores) is the focus, and the hypothesis explores how changes in the independent variable (teacher-led instructional time) affect it.
• In X-centered research designs, the independent variable is specified in the research question. Theories are used to determine potential dependent variables and the causal mechanisms at play.
• Example: “Implementing technology-based learning tools (IV) is likely to enhance student engagement in the classroom (DV), because interactive and multimedia content increases student interest and participation.”
• Hypothesis Explanation: The independent variable (technology-based learning tools) is the focus, with the hypothesis exploring its impact on a potential dependent variable (student engagement).
• Probabilistic hypotheses suggest that changes in the independent variable are likely to lead to changes in the dependent variable in a predictable manner, but not with absolute certainty.
• Example: “The more teachers engage in professional development programs (IV), the more their teaching effectiveness (DV) is likely to improve, because continuous training updates pedagogical skills and knowledge.”
• Hypothesis Explanation: This hypothesis implies a probable relationship between the extent of professional development (IV) and teaching effectiveness (DV).
• Deterministic hypotheses state that a specific change in the independent variable will lead to a specific change in the dependent variable, implying a more direct and certain relationship.
• Example: “If the school curriculum changes from traditional lecture-based methods to project-based learning (IV), then student collaboration skills (DV) are expected to improve because project-based learning inherently requires teamwork and peer interaction.”
• Hypothesis Explanation: This hypothesis presumes a direct and definite outcome (improvement in collaboration skills) resulting from a specific change in the teaching method.
• Example : “Students who identify as visual learners will score higher on tests that are presented in a visually rich format compared to tests presented in a text-only format.”
• Explanation : This hypothesis aims to describe the potential difference in test scores between visual learners taking visually rich tests and text-only tests, without implying a direct cause-and-effect relationship.
• Example : “Teaching method A will improve student performance more than method B.”
• Explanation : This hypothesis compares the effectiveness of two different teaching methods, suggesting that one will lead to better student performance than the other. It implies a direct comparison but does not necessarily establish a causal mechanism.
• Example : “Students with higher self-efficacy will show higher levels of academic achievement.”
• Explanation : This hypothesis predicts a relationship between the variable of self-efficacy and academic achievement. Unlike a causal hypothesis, it does not necessarily suggest that one variable causes changes in the other, but rather that they are related in some way.

Tips for developing research questions and hypotheses for research studies

• Perform a systematic literature review (if one has not been done) to increase knowledge and familiarity with the topic and to assist with research development.
• Learn about current trends and technological advances on the topic.
• Seek careful input from experts, mentors, colleagues, and collaborators to refine your research question as this will aid in developing the research question and guide the research study.
• Use the FINER criteria in the development of the research question.
• Ensure that the research question follows PICOT format.
• Develop a research hypothesis from the research question.
• Ensure that the research question and objectives are answerable, feasible, and clinically relevant.

If your research hypotheses are derived from your research questions, particularly when multiple hypotheses address a single question, it’s recommended to use both research questions and hypotheses. However, if this isn’t the case, using hypotheses over research questions is advised. It’s important to note these are general guidelines, not strict rules. If you opt not to use hypotheses, consult with your supervisor for the best approach.

Farrugia, P., Petrisor, B. A., Farrokhyar, F., & Bhandari, M. (2010). Practical tips for surgical research: Research questions, hypotheses and objectives.  Canadian journal of surgery. Journal canadien de chirurgie ,  53 (4), 278–281.

Hulley, S. B., Cummings, S. R., Browner, W. S., Grady, D., & Newman, T. B. (2007). Designing clinical research. Philadelphia.

Panke, D. (2018). Research design & method selection: Making good choices in the social sciences.  Research Design & Method Selection , 1-368.

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Perspective: Dimensions of the scientific method

Eberhard o. voit.

Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America

The scientific method has been guiding biological research for a long time. It not only prescribes the order and types of activities that give a scientific study validity and a stamp of approval but also has substantially shaped how we collectively think about the endeavor of investigating nature. The advent of high-throughput data generation, data mining, and advanced computational modeling has thrown the formerly undisputed, monolithic status of the scientific method into turmoil. On the one hand, the new approaches are clearly successful and expect the same acceptance as the traditional methods, but on the other hand, they replace much of the hypothesis-driven reasoning with inductive argumentation, which philosophers of science consider problematic. Intrigued by the enormous wealth of data and the power of machine learning, some scientists have even argued that significant correlations within datasets could make the entire quest for causation obsolete. Many of these issues have been passionately debated during the past two decades, often with scant agreement. It is proffered here that hypothesis-driven, data-mining–inspired, and “allochthonous” knowledge acquisition, based on mathematical and computational models, are vectors spanning a 3D space of an expanded scientific method. The combination of methods within this space will most certainly shape our thinking about nature, with implications for experimental design, peer review and funding, sharing of result, education, medical diagnostics, and even questions of litigation.

The traditional scientific method: Hypothesis-driven deduction

Research is the undisputed core activity defining science. Without research, the advancement of scientific knowledge would come to a screeching halt. While it is evident that researchers look for new information or insights, the term “research” is somewhat puzzling. Never mind the prefix “re,” which simply means “coming back and doing it again and again,” the word “search” seems to suggest that the research process is somewhat haphazard, that not much of a strategy is involved in the process. One might argue that research a few hundred years ago had the character of hoping for enough luck to find something new. The alchemists come to mind in their quest to turn mercury or lead into gold, or to discover an elixir for eternal youth, through methods we nowadays consider laughable.

Today’s sciences, in stark contrast, are clearly different. Yes, we still try to find something new—and may need a good dose of luck—but the process is anything but unstructured. In fact, it is prescribed in such rigor that it has been given the widely known moniker “scientific method.” This scientific method has deep roots going back to Aristotle and Herophilus (approximately 300 BC), Avicenna and Alhazen (approximately 1,000 AD), Grosseteste and Robert Bacon (approximately 1,250 AD), and many others, but solidified and crystallized into the gold standard of quality research during the 17th and 18th centuries [ 1 – 7 ]. In particular, Sir Francis Bacon (1561–1626) and René Descartes (1596–1650) are often considered the founders of the scientific method, because they insisted on careful, systematic observations of high quality, rather than metaphysical speculations that were en vogue among the scholars of the time [ 1 , 8 ]. In contrast to their peers, they strove for objectivity and insisted that observations, rather than an investigator’s preconceived ideas or superstitions, should be the basis for formulating a research idea [ 7 , 9 ].

Bacon and his 19th century follower John Stuart Mill explicitly proposed gaining knowledge through inductive reasoning: Based on carefully recorded observations, or from data obtained in a well-planned experiment, generalized assertions were to be made about similar yet (so far) unobserved phenomena [ 7 ]. Expressed differently, inductive reasoning attempts to derive general principles or laws directly from empirical evidence [ 10 ]. An example is the 19th century epigram of the physician Rudolf Virchow, Omnis cellula e cellula . There is no proof that indeed “every cell derives from a cell,” but like Virchow, we have made the observation time and again and never encountered anything suggesting otherwise.

In contrast to induction, the widely accepted, traditional scientific method is based on formulating and testing hypotheses. From the results of these tests, a deduction is made whether the hypothesis is presumably true or false. This type of hypotheticodeductive reasoning goes back to William Whewell, William Stanley Jevons, and Charles Peirce in the 19th century [ 1 ]. By the 20th century, the deductive, hypothesis-based scientific method had become deeply ingrained in the scientific psyche, and it is now taught as early as middle school in order to teach students valid means of discovery [ 8 , 11 , 12 ]. The scientific method has not only guided most research studies but also fundamentally influenced how we think about the process of scientific discovery.

Alas, because biology has almost no general laws, deduction in the strictest sense is difficult. It may therefore be preferable to use the term abduction, which refers to the logical inference toward the most plausible explanation, given a set of observations, although this explanation cannot be proven and is not necessarily true.

Over the decades, the hypothesis-based scientific method did experience variations here and there, but its conceptual scaffold remained essentially unchanged ( Fig 1 ). Its key is a process that begins with the formulation of a hypothesis that is to be rigorously tested, either in the wet lab or computationally; nonadherence to this principle is seen as lacking rigor and can lead to irreproducible results [ 1 , 13 – 15 ].

The central concept of the traditional scientific method is a falsifiable hypothesis regarding some phenomenon of interest. This hypothesis is to be tested experimentally or computationally. The test results support or refute the hypothesis, triggering a new round of hypothesis formulation and testing.

Going further, the prominent philosopher of science Sir Karl Popper argued that a scientific hypothesis can never be verified but that it can be disproved by a single counterexample. He therefore demanded that scientific hypotheses had to be falsifiable, because otherwise, testing would be moot [ 16 , 17 ] (see also [ 18 ]). As Gillies put it, “successful theories are those that survive elimination through falsification” [ 19 ]. Kelley and Scott agreed to some degree but warned that complete insistence on falsifiability is too restrictive as it would mark many computational techniques, statistical hypothesis testing, and even Darwin’s theory of evolution as nonscientific [ 20 ].

While the hypothesis-based scientific method has been very successful, its exclusive reliance on deductive reasoning is dangerous because according to the so-called Duhem–Quine thesis, hypothesis testing always involves an unknown number of explicit or implicit assumptions, some of which may steer the researcher away from hypotheses that seem implausible, although they are, in fact, true [ 21 ]. According to Kuhn, this bias can obstruct the recognition of paradigm shifts [ 22 ], which require the rethinking of previously accepted “truths” and the development of radically new ideas [ 23 , 24 ]. The testing of simultaneous alternative hypotheses [ 25 – 27 ] ameliorates this problem to some degree but not entirely.

The traditional scientific method is often presented in discrete steps, but it should really be seen as a form of critical thinking, subject to review and independent validation [ 8 ]. It has proven very influential, not only by prescribing valid experimentation, but also for affecting the way we attempt to understand nature [ 18 ], for teaching [ 8 , 12 ], reporting, publishing, and otherwise sharing information [ 28 ], for peer review and the awarding of funds by research-supporting agencies [ 29 , 30 ], for medical diagnostics [ 7 ], and even in litigation [ 31 ].

A second dimension of the scientific method: Data-mining–inspired induction

A major shift in biological experimentation occurred with the–omics revolution of the early 21st century. All of a sudden, it became feasible to perform high-throughput experiments that generated thousands of measurements, typically characterizing the expression or abundances of very many—if not all—genes, proteins, metabolites, or other biological quantities in a sample.

The strategy of measuring large numbers of items in a nontargeted fashion is fundamentally different from the traditional scientific method and constitutes a new, second dimension of the scientific method. Instead of hypothesizing and testing whether gene X is up-regulated under some altered condition, the leading question becomes which of the thousands of genes in a sample are up- or down-regulated. This shift in focus elevates the data to the supreme role of revealing novel insights by themselves ( Fig 2 ). As an important, generic advantage over the traditional strategy, this second dimension is free of a researcher’s preconceived notions regarding the molecular mechanisms governing the phenomenon of interest, which are otherwise the key to formulating a hypothesis. The prominent biologists Patrick Brown and David Botstein commented that “the patterns of expression will often suffice to begin de novo discovery of potential gene functions” [ 32 ].

Data-driven research begins with an untargeted exploration, in which the data speak for themselves. Machine learning extracts patterns from the data, which suggest hypotheses that are to be tested in the lab or computationally.

This data-driven, discovery-generating approach is at once appealing and challenging. On the one hand, very many data are explored simultaneously and essentially without bias. On the other hand, the large datasets supporting this approach create a genuine challenge to understanding and interpreting the experimental results because the thousands of data points, often superimposed with a fair amount of noise, make it difficult to detect meaningful differences between sample and control. This situation can only be addressed with computational methods that first “clean” the data, for instance, through the statistically valid removal of outliers, and then use machine learning to identify statistically significant, distinguishing molecular profiles or signatures. In favorable cases, such signatures point to specific biological pathways, whereas other signatures defy direct explanation but may become the launch pad for follow-up investigations [ 33 ].

Today’s scientists are very familiar with this discovery-driven exploration of “what’s out there” and might consider it a quaint quirk of history that this strategy was at first widely chastised and ridiculed as a “fishing expedition” [ 30 , 34 ]. Strict traditionalists were outraged that rigor was leaving science with the new approach and that sufficient guidelines were unavailable to assure the validity and reproducibility of results [ 10 , 35 , 36 ].

From the view point of philosophy of science, this second dimension of the scientific method uses inductive reasoning and reflects Bacon’s idea that observations can and should dictate the research question to be investigated [ 1 , 7 ]. Allen [ 36 ] forcefully rejected this type of reasoning, stating “the thinking goes, we can now expect computer programs to derive significance, relevance and meaning from chunks of information, be they nucleotide sequences or gene expression profiles… In contrast with this view, many are convinced that no purely logical process can turn observation into understanding.” His conviction goes back to the 18th century philosopher David Hume and again to Popper, who identified as the overriding problem with inductive reasoning that it can never truly reveal causality, even if a phenomenon is observed time and again [ 16 , 17 , 37 , 38 ]. No number of observations, even if they always have the same result, can guard against an exception that would violate the generality of a law inferred from these observations [ 1 , 35 ]. Worse, Popper argued, through inference by induction, we cannot even know the probability of something being true [ 10 , 17 , 36 ].

Others argued that data-driven and hypothesis-driven research actually do not differ all that much in principle, as long as there is cycling between developing new ideas and testing them with care [ 27 ]. In fact, Kell and Oliver [ 34 ] maintained that the exclusive acceptance of hypothesis-driven programs misrepresents the complexities of biological knowledge generation. Similarly refuting the prominent rule of deduction, Platt [ 26 ] and Beard and Kushmerick [ 27 ] argued that repeated inductive reasoning, called strong inference, corresponds to a logically sound decision tree of disproving or refining hypotheses that can rapidly yield firm conclusions; nonetheless, Platt had to admit that inductive inference is not as certain as deduction, because it projects into the unknown. Lander compared the task of obtaining causality by induction to the problem of inferring the design of a microprocessor from input-output readings, which in a strict sense is impossible, because the microprocessor could be arbitrarily complicated; even so, inference often leads to novel insights and therefore is valuable [ 39 ].

An interesting special case of almost pure inductive reasoning is epidemiology, where hypothesis-driven reasoning is rare and instead, the fundamental question is whether data-based evidence is sufficient to associate health risks with specific causes [ 31 , 34 ].

Recent advances in machine learning and “big-data” mining have driven the use of inductive reasoning to unprecedented heights. As an example, machine learning can greatly assist in the discovery of patterns, for instance, in biological sequences [ 40 ]. Going a step further, a pithy article by Andersen [ 41 ] proffered that we may not need to look for causality or mechanistic explanations anymore if we just have enough correlation: “With enough data, the numbers speak for themselves, correlation replaces causation, and science can advance even without coherent models or unified theories.”

Of course, the proposal to abandon the quest for causality caused pushback on philosophical as well as mathematical grounds. Allen [ 10 , 35 ] considered the idea “absurd” that data analysis could enhance understanding in the absence of a hypothesis. He felt confident “that even the formidable combination of computing power with ease of access to data cannot produce a qualitative shift in the way that we do science: the making of hypotheses remains an indispensable component in the growth of knowledge” [ 36 ]. Succi and Coveney [ 42 ] refuted the “most extravagant claims” of big-data proponents very differently, namely by analyzing the theories on which machine learning is founded. They contrasted the assumptions underlying these theories, such as the law of large numbers, with the mathematical reality of complex biological systems. Specifically, they carefully identified genuine features of these systems, such as nonlinearities, nonlocality of effects, fractal aspects, and high dimensionality, and argued that they fundamentally violate some of the statistical assumptions implicitly underlying big-data analysis, like independence of events. They concluded that these discrepancies “may lead to false expectations and, at their nadir, even to dangerous social, economical and political manipulation.” To ameliorate the situation, the field of big-data analysis would need new strong theorems characterizing the validity of its methods and the numbers of data required for obtaining reliable insights. Succi and Coveney go as far as stating that too many data are just as bad as insufficient data [ 42 ].

While philosophical doubts regarding inductive methods will always persist, one cannot deny that -omics-based, high-throughput studies, combined with machine learning and big-data analysis, have been very successful [ 43 ]. Yes, induction cannot truly reveal general laws, no matter how large the datasets, but they do provide insights that are very different from what science had offered before and may at least suggest novel patterns, trends, or principles. As a case in point, if many transcriptomic studies indicate that a particular gene set is involved in certain classes of phenomena, there is probably some truth to the observation, even though it is not mathematically provable. Kepler’s laws of astronomy were arguably derived solely from inductive reasoning [ 34 ].

Notwithstanding the opposing views on inductive methods, successful strategies shape how we think about science. Thus, to take advantage of all experimental options while ensuring quality of research, we must not allow that “anything goes” but instead identify and characterize standard operating procedures and controls that render this emerging scientific method valid and reproducible. A laudable step in this direction was the wide acceptance of “minimum information about a microarray experiment” (MIAME) standards for microarray experiments [ 44 ].

A third dimension of the scientific method: Allochthonous reasoning

Parallel to the blossoming of molecular biology and the rapid rise in the power and availability of computing in the late 20th century, the use of mathematical and computational models became increasingly recognized as relevant and beneficial for understanding biological phenomena. Indeed, mathematical models eventually achieved cornerstone status in the new field of computational systems biology.

Mathematical modeling has been used as a tool of biological analysis for a long time [ 27 , 45 – 48 ]. Interesting for the discussion here is that the use of mathematical and computational modeling in biology follows a scientific approach that is distinctly different from the traditional and the data-driven methods, because it is distributed over two entirely separate domains of knowledge. One consists of the biological reality of DNA, elephants, and roses, whereas the other is the world of mathematics, which is governed by numbers, symbols, theorems, and abstract work protocols. Because the ways of thinking—and even the languages—are different in these two realms, I suggest calling this type of knowledge acquisition “allochthonous” (literally Greek: in or from a “piece of land different from where one is at home”; one could perhaps translate it into modern lingo as “outside one’s comfort zone”). De facto, most allochthonous reasoning in biology presently refers to mathematics and computing, but one might also consider, for instance, the application of methods from linguistics in the analysis of DNA sequences or proteins [ 49 ].

One could argue that biologists have employed “models” for a long time, for instance, in the form of “model organisms,” cell lines, or in vitro experiments, which more or less faithfully reflect features of the organisms of true interest but are easier to manipulate. However, this type of biological model use is rather different from allochthonous reasoning, as it does not leave the realm of biology and uses the same language and often similar methodologies.

A brief discussion of three experiences from our lab may illustrate the benefits of allochthonous reasoning. (1) In a case study of renal cell carcinoma, a dynamic model was able to explain an observed yet nonintuitive metabolic profile in terms of the enzymatic reaction steps that had been altered during the disease [ 50 ]. (2) A transcriptome analysis had identified several genes as displaying significantly different expression patterns during malaria infection in comparison to the state of health. Considered by themselves and focusing solely on genes coding for specific enzymes of purine metabolism, the findings showed patterns that did not make sense. However, integrating the changes in a dynamic model revealed that purine metabolism globally shifted, in response to malaria, from guanine compounds to adenine, inosine, and hypoxanthine [ 51 ]. (3) Data capturing the dynamics of malaria parasites suggested growth rates that were biologically impossible. Speculation regarding possible explanations led to the hypothesis that many parasite-harboring red blood cells might “hide” from circulation and therewith from detection in the blood stream. While experimental testing of the feasibility of the hypothesis would have been expensive, a dynamic model confirmed that such a concealment mechanism could indeed quantitatively explain the apparently very high growth rates [ 52 ]. In all three cases, the insights gained inductively from computational modeling would have been difficult to obtain purely with experimental laboratory methods. Purely deductive allochthonous reasoning is the ultimate goal of the search for design and operating principles [ 53 – 55 ], which strives to explain why certain structures or functions are employed by nature time and again. An example is a linear metabolic pathway, in which feedback inhibition is essentially always exerted on the first step [ 56 , 57 ]. This generality allows the deduction that a so far unstudied linear pathway is most likely (or even certain to be) inhibited at the first step. Not strictly deductive—but rather abductive—was a study in our lab in which we analyzed time series data with a mathematical model that allowed us to infer the most likely regulatory structure of a metabolic pathway [ 58 , 59 ].

A typical allochthonous investigation begins in the realm of biology with the formulation of a hypothesis ( Fig 3 ). Instead of testing this hypothesis with laboratory experiments, the system encompassing the hypothesis is moved into the realm of mathematics. This move requires two sets of ingredients. One set consists of the simplification and abstraction of the biological system: Any distracting details that seem unrelated to the hypothesis and its context are omitted or represented collectively with other details. This simplification step carries the greatest risk of the entire modeling approach, as omission of seemingly negligible but, in truth, important details can easily lead to wrong results. The second set of ingredients consists of correspondence rules that translate every biological component or process into the language of mathematics [ 60 , 61 ].

This mathematical and computational approach is distributed over two realms, which are connected by correspondence rules.

Once the system is translated, it has become an entirely mathematical construct that can be analyzed purely with mathematical and computational means. The results of this analysis are also strictly mathematical. They typically consist of values of variables, magnitudes of processes, sensitivity patterns, signs of eigenvalues, or qualitative features like the onset of oscillations or the potential for limit cycles. Correspondence rules are used again to move these results back into the realm of biology. As an example, the mathematical result that “two eigenvalues have positive real parts” does not make much sense to many biologists, whereas the interpretation that “the system is not stable at the steady state in question” is readily explained. New biological insights may lead to new hypotheses, which are tested either by experiments or by returning once more to the realm of mathematics. The model design, diagnosis, refinements, and validation consist of several phases, which have been discussed widely in the biomathematical literature. Importantly, each iteration of a typical modeling analysis consists of a move from the biological to the mathematical realm and back.

The reasoning within the realm of mathematics is often deductive, in the form of an Aristotelian syllogism, such as the well-known “All men are mortal; Socrates is a man; therefore, Socrates is mortal.” However, the reasoning may also be inductive, as it is the case with large-scale Monte-Carlo simulations that generate arbitrarily many “observations,” although they cannot reveal universal principles or theorems. An example is a simulation randomly drawing numbers in an attempt to show that every real number has an inverse. The simulation will always attest to this hypothesis but fail to discover the truth because it will never randomly draw 0. Generically, computational models may be considered sets of hypotheses, formulated as equations or as algorithms that reflect our perception of a complex system [ 27 ].

Impact of the multidimensional scientific method on learning

Almost all we know in biology has come from observation, experimentation, and interpretation. The traditional scientific method not only offered clear guidance for this knowledge gathering, but it also fundamentally shaped the way we think about the exploration of nature. When presented with a new research question, scientists were trained to think immediately in terms of hypotheses and alternatives, pondering the best feasible ways of testing them, and designing in their minds strong controls that would limit the effects of known or unknown confounders. Shaped by the rigidity of this ever-repeating process, our thinking became trained to move forward one well-planned step at a time. This modus operandi was rigid and exact. It also minimized the erroneous pursuit of long speculative lines of thought, because every step required testing before a new hypothesis was formed. While effective, the process was also very slow and driven by ingenuity—as well as bias—on the scientist’s part. This bias was sometimes a hindrance to necessary paradigm shifts [ 22 ].

High-throughput data generation, big-data analysis, and mathematical-computational modeling changed all that within a few decades. In particular, the acceptance of inductive principles and of the allochthonous use of nonbiological strategies to answer biological questions created an unprecedented mix of successes and chaos. To the horror of traditionalists, the importance of hypotheses became minimized, and the suggestion spread that the data would speak for themselves [ 36 ]. Importantly, within this fog of “anything goes,” the fundamental question arose how to determine whether an experiment was valid.

Because agreed-upon operating procedures affect research progress and interpretation, thinking, teaching, and sharing of results, this question requires a deconvolution of scientific strategies. Here I proffer that the single scientific method of the past should be expanded toward a vector space of scientific methods, with spanning vectors that correspond to different dimensions of the scientific method ( Fig 4 ).

The traditional hypothesis-based deductive scientific method is expanded into a 3D space that allows for synergistic blends of methods that include data-mining–inspired, inductive knowledge acquisition, and mathematical model-based, allochthonous reasoning.

Obviously, all three dimensions have their advantages and drawbacks. The traditional, hypothesis-driven deductive method is philosophically “clean,” except that it is confounded by preconceptions and assumptions. The data-mining–inspired inductive method cannot offer universal truths but helps us explore very large spaces of factors that contribute to a phenomenon. Allochthonous, model-based reasoning can be performed mentally, with paper and pencil, through rigorous analysis, or with a host of computational methods that are precise and disprovable [ 27 ]. At the same time, they are incomparable faster, cheaper, and much more comprehensive than experiments in molecular biology. This reduction in cost and time, and the increase in coverage, may eventually have far-reaching consequences, as we can already fathom from much of modern physics.

Due to its long history, the traditional dimension of the scientific method is supported by clear and very strong standard operating procedures. Similarly, strong procedures need to be developed for the other two dimensions. The MIAME rules for microarray analysis provide an excellent example [ 44 ]. On the mathematical modeling front, no such rules are generally accepted yet, but trends toward them seem to emerge at the horizon. For instance, it seems to be becoming common practice to include sensitivity analyses in typical modeling studies and to assess the identifiability or sloppiness of ensembles of parameter combinations that fit a given dataset well [ 62 , 63 ].

From a philosophical point of view, it seems unlikely that objections against inductive reasoning will disappear. However, instead of pitting hypothesis-based deductive reasoning against inductivism, it seems more beneficial to determine how the different methods can be synergistically blended ( cf . [ 18 , 27 , 34 , 42 ]) as linear combinations of the three vectors of knowledge acquisition ( Fig 4 ). It is at this point unclear to what degree the identified three dimensions are truly independent of each other, whether additional dimensions should be added [ 24 ], or whether the different versions could be amalgamated into a single scientific method [ 18 ], especially if it is loosely defined as a form of critical thinking [ 8 ]. Nobel Laureate Percy Bridgman even concluded that “science is what scientists do, and there are as many scientific methods as there are individual scientists” [ 8 , 64 ].

Combinations of the three spanning vectors of the scientific method have been emerging for some time. Many biologists already use inductive high-throughput methods to develop specific hypotheses that are subsequently tested with deductive or further inductive methods [ 34 , 65 ]. In terms of including mathematical modeling, physics and geology have been leading the way for a long time, often by beginning an investigation in theory, before any actual experiment is performed. It will benefit biology to look into this strategy and to develop best practices of allochthonous reasoning.

The blending of methods may take quite different shapes. Early on, Ideker and colleagues [ 65 ] proposed an integrated experimental approach for pathway analysis that offered a glimpse of new experimental strategies within the space of scientific methods. In a similar vein, Covert and colleagues [ 66 ] included computational methods into such an integrated approach. Additional examples of blended analyses in systems biology can be seen in other works, such as [ 43 , 67 – 73 ]. Generically, it is often beneficial to start with big data, determine patterns in associations and correlations, then switch to the mathematical realm in order to filter out spurious correlations in a high-throughput fashion. If this procedure is executed in an iterative manner, the “surviving” associations have an increased level of confidence and are good candidates for further experimental or computational testing (personal communication from S. Chandrasekaran).

If each component of a blended scientific method follows strict, commonly agreed guidelines, “linear combinations” within the 3D space can also be checked objectively, per deconvolution. In addition, guidelines for synergistic blends of component procedures should be developed. If we carefully monitor such blends, time will presumably indicate which method is best for which task and how the different approaches optimally inform each other. For instance, it will be interesting to study whether there is an optimal sequence of experiments along the three axes for a particular class of tasks. Big-data analysis together with inductive reasoning might be optimal for creating initial hypotheses and possibly refuting wrong speculations (“we had thought this gene would be involved, but apparently it isn’t”). If the logic of an emerging hypotheses can be tested with mathematical and computational tools, it will almost certainly be faster and cheaper than an immediate launch into wet-lab experimentation. It is also likely that mathematical reasoning will be able to refute some apparently feasible hypothesis and suggest amendments. Ultimately, the “surviving” hypotheses must still be tested for validity through conventional experiments. Deconvolving current practices and optimizing the combination of methods within the 3D or higher-dimensional space of scientific methods will likely result in better planning of experiments and in synergistic blends of approaches that have the potential capacity of addressing some of the grand challenges in biology.

Acknowledgments

The author is very grateful to Dr. Sriram Chandrasekaran and Ms. Carla Kumbale for superb suggestions and invaluable feedback.

Funding Statement

This work was supported in part by grants from the National Science Foundation ( https://www.nsf.gov/div/index.jsp?div=MCB ) grant NSF-MCB-1517588 (PI: EOV), NSF-MCB-1615373 (PI: Diana Downs) and the National Institute of Environmental Health Sciences ( https://www.niehs.nih.gov/ ) grant NIH-2P30ES019776-05 (PI: Carmen Marsit). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Brief report: Benevolent childhood experiences, parental toxic stress, and maternal emotional reaction to infant crying

• Published: 30 May 2024

Cite this article

• Joana Baptista   ORCID: orcid.org/0000-0002-7581-3909 1 , 2 , 3 ,
• Fanni Oláh 1 ,
• Cláudia Ramos 1 ,
• Bárbara Claro 1 &
• Inês Almeida 1  

Infant crying elicits varied parental responses. While maternal stress has been associated with increased frustration in response to crying, early positive experiences may foster child-oriented reactions. This study explored the links between Benevolent Childhood Experiences (BCEs), parental toxic stress, and maternal emotional reactions to crying. The sample included 95 mothers with infants aged 9 to 15 months. Maternal responses were assessed using the My Emotions Questionnaire (Leerkes & Qu in Infant Mental Health Journal, 41 (1), 94-107, 2020 ). Mothers also completed the Benevolent Childhood Experiences Scale (Narayan et al. in Child Abuse & Neglect, 78 , 19–30, 2018 ) and the Functional Impact of Toxic Stress for Parents (Moreno et al. Children and Youth Services Review, 131 , 106280, 2021 ) to measure BCEs and toxic stress, respectively. Contrary to the hypothesis, BCEs were not significantly linked to child-centered emotions. Parental toxic stress emerged as a significant predictor of maternal anxiety and frustration in response to crying. While this exploratory studied provided valuable insights, more research on the mechanism underlying reactions to crying is warranted.

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The data that support the findings of this study are available on request from the corresponding author. The data is not publicly available due to privacy or ethical restrictions.

Bornstein, M. H., Putnick, D. L., Rigo, P., Esposito, G., Swain, J. E., Suwalsky, J. T., & Venuti, P. (2017). Neurobiology of culturally common maternal responses to infant cry. Pnas , 114 (45), E9465–E9473. https://doi.org/10.1073/pnas.1712022114

Article   PubMed   PubMed Central   Google Scholar  

Håkansson, U., Watten, R., Söderström, K., Skårderud, F., & Øie, M. G. (2018). Adverse and adaptive childhood experiences are associated with parental reflective functioning in mothers with substance use disorder. Child Abuse & Neglect , 81 , 259–273. https://doi.org/10.1016/j.chiabu.2018.05.007

Article   Google Scholar  

Hiraoka, D., & Nomura, M. (2017). Would situational stress be harmful for anyone? The influence of situational factors and trait empathy on women’s response to infant crying. Infant Behavior and Development , 48 , 147–156. https://doi.org/10.1016/j.infbeh.2017.04.005

Article   PubMed   Google Scholar  

Leerkes, E. M., & Qu, J. (2020). The my emotions Questionnaire: A self-report of mothers’ emotional responses to infant crying. Infant Mental Health Journal , 41 (1), 94–107. https://doi.org/10.1002/imhj.21830

Leerkes, E. M., Bailes, L., Swingler, M. M., Augustine, M. A., & Norcross, P. L. (2021). A comprehensive model of women’s social cognition and responsiveness to infant crying: Integrating personality, emotion, executive function, and sleep. Infant Behavior and Development , 64 , 101577. https://doi.org/10.1016/j.infbeh.2021.101577

Lyons-Ruth, K., Bureau, J. F., Easterbrooks, M. A., Obsuth, I., Hennighausen, K., & Vulliez-Coady, L. (2013). Parsing the construct of maternal insensitivity: Distinct longitudinal pathways associated with early maternal withdrawal. Attachment & Human Development , 15 (5–6), 562–582. https://doi.org/10.1080/14616734.2013.841051

McElwain, N. L., & Booth-LaForce, C. (2006). Maternal sensitivity to infant distress and nondistress as predictors of infant-mother attachment security. Journal of Family Psychology , 20 (2), 247–255. https://doi.org/10.1037/0893-3200.20.2.247

Merrick, J. S., Narayan, A. J., Atzl, V. M., Harris, W. W., & Lieberman, A. F. (2020). Type versus timing of adverse and benevolent childhood experiences for pregnant women’s psychological and reproductive health. Children and Youth Services Review , 114 , 105056. https://doi.org/10.1016/j.childyouth.2020.105056

Moreno, A. J., Byers, K., Monahan, E., Robinson, J. L., & McCrae, J. (2021). Beyond overwhelmed: A new measure of the functional impact of toxic stress on parents of young children. Children and Youth Services Review , 131 , 106280. https://doi.org/10.1016/j.childyouth.2021.106280

Morris, A. S., Hays-Grudo, J., Zapata, M. I., Treat, A., & Kerr, K. L. (2021). Adverse and protective childhood experiences and parenting attitudes: The role of cumulative protection in understanding resilience. Adversity and Resilience Science , 2 (3), 181–192. https://doi.org/10.1007/s42844-021-00036-8

Murray, A. D. (1979). Infant crying as an elicitor of parental behavior: An examination of two models. Psychological Bulletin , 86 , 191–215. https://doi.org/10.1037/0033-2909.86.1.191

Narayan, A. J., Ippen, C. G., Harris, W. W., & Lieberman, A. F. (2017). Assessing angels in the nursery: A pilot study of childhood memories of benevolent caregiving as protective influences. Infant Mental Health Journal , 38 (4), 461–474. https://doi.org/10.1002/imhj.21653

Narayan, A. J., Rivera, L. M., Bernstein, R. E., Harris, W. W., & Lieberman, A. F. (2018). Positive childhood experiences predict less psychopathology and stress in pregnant women with childhood adversity: A pilot study of the benevolent childhood experiences (BCEs) scale. Child Abuse & Neglect , 78 , 19–30. https://doi.org/10.1016/j.chiabu.2017.09.022

Pizzagalli, D. A. (2014). Depression, stress, and anhedonia: Toward a synthesis and integrated model. Annual Review of Clinical Psychology , 10 , 393–423. https://doi.org/10.1146/annurev-clinpsy-050212-185606

Rahman, B., Lee, M., & Rilling, J. K. (2023). Frustration responses of single and partnered mothers to prolonged infant crying. Journal of Family Psychology , 37 (5), 699–708. https://doi.org/10.1037/fam0001077

Strathearn, L., & Kim, S. (2013). Mothers’ amygdala response to positive or negative infant affect is modulated by personal relevance. Frontiers in Neuroscience , 7 , 1–10. https://doi.org/10.3389/fnins.2013.00176

Tognasso, G., Gorla, L., Ambrosini, C., Figurella, F., De Carli, P., Parolin, L., Sarracino, D., & Santona, A. (2022). Parenting stress, maternal self-efficacy and confidence in caretaking in a sample of mothers with newborns (0–1 Month). International Journal of Environmental Research and Public Health , 19 (15), 9651. https://doi.org/10.3390/ijerph19159651

Wang, W., Dong, Y., Liu, X., Bai, Y., & Zang, L. (2020). The effect of parents’ education on the academic and non-cognitive outcomes of their children: Evidence from China. Children and Youth Services Review , 117 , 105307. https://doi.org/10.1016/j.childyouth.2020.105307

Zeifman, D. M., & St James-Roberts, I. (2017). Parenting the crying infant. Current Opinion in Psychology , 15 , 149–154. https://doi.org/10.1016/j.copsyc.2017.02.009

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Acknowledgements

This work was supported by CIS-IUL by Portuguese national funds through FCT— Fundação para a Ciência e a Tecnologia, I.P. ( https://doi.org/10.54499/UIDB/03125/2020 ). The authors are very grateful to all the mothers who generously participated in this study.

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Joana Baptista, Fanni Oláh, Cláudia Ramos, Bárbara Claro & Inês Almeida

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Baptista, J., Oláh, F., Ramos, C. et al. Brief report: Benevolent childhood experiences, parental toxic stress, and maternal emotional reaction to infant crying. Curr Psychol (2024). https://doi.org/10.1007/s12144-024-06176-1

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Accepted : 20 May 2024

Published : 30 May 2024

DOI : https://doi.org/10.1007/s12144-024-06176-1

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