null and alternative hypothesis chi square

Hypothesis Testing - Chi Squared Test

Lisa Sullivan, PhD

Professor of Biostatistics

Boston University School of Public Health

Introduction

This module will continue the discussion of hypothesis testing, where a specific statement or hypothesis is generated about a population parameter, and sample statistics are used to assess the likelihood that the hypothesis is true. The hypothesis is based on available information and the investigator's belief about the population parameters. The specific tests considered here are called chi-square tests and are appropriate when the outcome is discrete (dichotomous, ordinal or categorical). For example, in some clinical trials the outcome is a classification such as hypertensive, pre-hypertensive or normotensive. We could use the same classification in an observational study such as the Framingham Heart Study to compare men and women in terms of their blood pressure status - again using the classification of hypertensive, pre-hypertensive or normotensive status.  

The technique to analyze a discrete outcome uses what is called a chi-square test. Specifically, the test statistic follows a chi-square probability distribution. We will consider chi-square tests here with one, two and more than two independent comparison groups.

Learning Objectives

After completing this module, the student will be able to:

• Perform chi-square tests by hand
• Appropriately interpret results of chi-square tests
• Identify the appropriate hypothesis testing procedure based on type of outcome variable and number of samples

Tests with One Sample, Discrete Outcome

Here we consider hypothesis testing with a discrete outcome variable in a single population. Discrete variables are variables that take on more than two distinct responses or categories and the responses can be ordered or unordered (i.e., the outcome can be ordinal or categorical). The procedure we describe here can be used for dichotomous (exactly 2 response options), ordinal or categorical discrete outcomes and the objective is to compare the distribution of responses, or the proportions of participants in each response category, to a known distribution. The known distribution is derived from another study or report and it is again important in setting up the hypotheses that the comparator distribution specified in the null hypothesis is a fair comparison. The comparator is sometimes called an external or a historical control.   

In one sample tests for a discrete outcome, we set up our hypotheses against an appropriate comparator. We select a sample and compute descriptive statistics on the sample data. Specifically, we compute the sample size (n) and the proportions of participants in each response

Test Statistic for Testing H 0 : p 1 = p 10 , p 2 = p 20 , ..., p k = p k0

We find the critical value in a table of probabilities for the chi-square distribution with degrees of freedom (df) = k-1. In the test statistic, O = observed frequency and E=expected frequency in each of the response categories. The observed frequencies are those observed in the sample and the expected frequencies are computed as described below. χ 2 (chi-square) is another probability distribution and ranges from 0 to ∞. The test above statistic formula above is appropriate for large samples, defined as expected frequencies of at least 5 in each of the response categories.  

When we conduct a χ 2 test, we compare the observed frequencies in each response category to the frequencies we would expect if the null hypothesis were true. These expected frequencies are determined by allocating the sample to the response categories according to the distribution specified in H 0 . This is done by multiplying the observed sample size (n) by the proportions specified in the null hypothesis (p 10 , p 20 , ..., p k0 ). To ensure that the sample size is appropriate for the use of the test statistic above, we need to ensure that the following: min(np 10 , n p 20 , ..., n p k0 ) > 5.  

The test of hypothesis with a discrete outcome measured in a single sample, where the goal is to assess whether the distribution of responses follows a known distribution, is called the χ 2 goodness-of-fit test. As the name indicates, the idea is to assess whether the pattern or distribution of responses in the sample "fits" a specified population (external or historical) distribution. In the next example we illustrate the test. As we work through the example, we provide additional details related to the use of this new test statistic.  

A University conducted a survey of its recent graduates to collect demographic and health information for future planning purposes as well as to assess students' satisfaction with their undergraduate experiences. The survey revealed that a substantial proportion of students were not engaging in regular exercise, many felt their nutrition was poor and a substantial number were smoking. In response to a question on regular exercise, 60% of all graduates reported getting no regular exercise, 25% reported exercising sporadically and 15% reported exercising regularly as undergraduates. The next year the University launched a health promotion campaign on campus in an attempt to increase health behaviors among undergraduates. The program included modules on exercise, nutrition and smoking cessation. To evaluate the impact of the program, the University again surveyed graduates and asked the same questions. The survey was completed by 470 graduates and the following data were collected on the exercise question:

Based on the data, is there evidence of a shift in the distribution of responses to the exercise question following the implementation of the health promotion campaign on campus? Run the test at a 5% level of significance.

In this example, we have one sample and a discrete (ordinal) outcome variable (with three response options). We specifically want to compare the distribution of responses in the sample to the distribution reported the previous year (i.e., 60%, 25%, 15% reporting no, sporadic and regular exercise, respectively). We now run the test using the five-step approach.  

• Step 1. Set up hypotheses and determine level of significance.

The null hypothesis again represents the "no change" or "no difference" situation. If the health promotion campaign has no impact then we expect the distribution of responses to the exercise question to be the same as that measured prior to the implementation of the program.

H 0 : p 1 =0.60, p 2 =0.25, p 3 =0.15,  or equivalently H 0 : Distribution of responses is 0.60, 0.25, 0.15  

H 1 :   H 0 is false.          α =0.05

Notice that the research hypothesis is written in words rather than in symbols. The research hypothesis as stated captures any difference in the distribution of responses from that specified in the null hypothesis. We do not specify a specific alternative distribution, instead we are testing whether the sample data "fit" the distribution in H 0 or not. With the χ 2 goodness-of-fit test there is no upper or lower tailed version of the test.

• Step 2. Select the appropriate test statistic.  

The test statistic is:

We must first assess whether the sample size is adequate. Specifically, we need to check min(np 0 , np 1, ..., n p k ) > 5. The sample size here is n=470 and the proportions specified in the null hypothesis are 0.60, 0.25 and 0.15. Thus, min( 470(0.65), 470(0.25), 470(0.15))=min(282, 117.5, 70.5)=70.5. The sample size is more than adequate so the formula can be used.

• Step 3. Set up decision rule.  

The decision rule for the χ 2 test depends on the level of significance and the degrees of freedom, defined as degrees of freedom (df) = k-1 (where k is the number of response categories). If the null hypothesis is true, the observed and expected frequencies will be close in value and the χ 2 statistic will be close to zero. If the null hypothesis is false, then the χ 2 statistic will be large. Critical values can be found in a table of probabilities for the χ 2 distribution. Here we have df=k-1=3-1=2 and a 5% level of significance. The appropriate critical value is 5.99, and the decision rule is as follows: Reject H 0 if χ 2 > 5.99.

• Step 4. Compute the test statistic.  

We now compute the expected frequencies using the sample size and the proportions specified in the null hypothesis. We then substitute the sample data (observed frequencies) and the expected frequencies into the formula for the test statistic identified in Step 2. The computations can be organized as follows.

Notice that the expected frequencies are taken to one decimal place and that the sum of the observed frequencies is equal to the sum of the expected frequencies. The test statistic is computed as follows:

• Step 5. Conclusion.  

We reject H 0 because 8.46 > 5.99. We have statistically significant evidence at α=0.05 to show that H 0 is false, or that the distribution of responses is not 0.60, 0.25, 0.15.  The p-value is p < 0.005.  

In the χ 2 goodness-of-fit test, we conclude that either the distribution specified in H 0 is false (when we reject H 0 ) or that we do not have sufficient evidence to show that the distribution specified in H 0 is false (when we fail to reject H 0 ). Here, we reject H 0 and concluded that the distribution of responses to the exercise question following the implementation of the health promotion campaign was not the same as the distribution prior. The test itself does not provide details of how the distribution has shifted. A comparison of the observed and expected frequencies will provide some insight into the shift (when the null hypothesis is rejected). Does it appear that the health promotion campaign was effective?  

Consider the following: 

If the null hypothesis were true (i.e., no change from the prior year) we would have expected more students to fall in the "No Regular Exercise" category and fewer in the "Regular Exercise" categories. In the sample, 255/470 = 54% reported no regular exercise and 90/470=19% reported regular exercise. Thus, there is a shift toward more regular exercise following the implementation of the health promotion campaign. There is evidence of a statistical difference, is this a meaningful difference? Is there room for improvement?

The National Center for Health Statistics (NCHS) provided data on the distribution of weight (in categories) among Americans in 2002. The distribution was based on specific values of body mass index (BMI) computed as weight in kilograms over height in meters squared. Underweight was defined as BMI< 18.5, Normal weight as BMI between 18.5 and 24.9, overweight as BMI between 25 and 29.9 and obese as BMI of 30 or greater. Americans in 2002 were distributed as follows: 2% Underweight, 39% Normal Weight, 36% Overweight, and 23% Obese. Suppose we want to assess whether the distribution of BMI is different in the Framingham Offspring sample. Using data from the n=3,326 participants who attended the seventh examination of the Offspring in the Framingham Heart Study we created the BMI categories as defined and observed the following:

• Step 1.  Set up hypotheses and determine level of significance.

H 0 : p 1 =0.02, p 2 =0.39, p 3 =0.36, p 4 =0.23     or equivalently

H 0 : Distribution of responses is 0.02, 0.39, 0.36, 0.23

H 1 :   H 0 is false.        α=0.05

The formula for the test statistic is:

We must assess whether the sample size is adequate. Specifically, we need to check min(np 0 , np 1, ..., n p k ) > 5. The sample size here is n=3,326 and the proportions specified in the null hypothesis are 0.02, 0.39, 0.36 and 0.23. Thus, min( 3326(0.02), 3326(0.39), 3326(0.36), 3326(0.23))=min(66.5, 1297.1, 1197.4, 765.0)=66.5. The sample size is more than adequate, so the formula can be used.

Here we have df=k-1=4-1=3 and a 5% level of significance. The appropriate critical value is 7.81 and the decision rule is as follows: Reject H 0 if χ 2 > 7.81.

We now compute the expected frequencies using the sample size and the proportions specified in the null hypothesis. We then substitute the sample data (observed frequencies) into the formula for the test statistic identified in Step 2. We organize the computations in the following table.

The test statistic is computed as follows:

We reject H 0 because 233.53 > 7.81. We have statistically significant evidence at α=0.05 to show that H 0 is false or that the distribution of BMI in Framingham is different from the national data reported in 2002, p < 0.005.  

Again, the χ 2   goodness-of-fit test allows us to assess whether the distribution of responses "fits" a specified distribution. Here we show that the distribution of BMI in the Framingham Offspring Study is different from the national distribution. To understand the nature of the difference we can compare observed and expected frequencies or observed and expected proportions (or percentages). The frequencies are large because of the large sample size, the observed percentages of patients in the Framingham sample are as follows: 0.6% underweight, 28% normal weight, 41% overweight and 30% obese. In the Framingham Offspring sample there are higher percentages of overweight and obese persons (41% and 30% in Framingham as compared to 36% and 23% in the national data), and lower proportions of underweight and normal weight persons (0.6% and 28% in Framingham as compared to 2% and 39% in the national data). Are these meaningful differences?

In the module on hypothesis testing for means and proportions, we discussed hypothesis testing applications with a dichotomous outcome variable in a single population. We presented a test using a test statistic Z to test whether an observed (sample) proportion differed significantly from a historical or external comparator. The chi-square goodness-of-fit test can also be used with a dichotomous outcome and the results are mathematically equivalent.  

In the prior module, we considered the following example. Here we show the equivalence to the chi-square goodness-of-fit test.

The NCHS report indicated that in 2002, 75% of children aged 2 to 17 saw a dentist in the past year. An investigator wants to assess whether use of dental services is similar in children living in the city of Boston. A sample of 125 children aged 2 to 17 living in Boston are surveyed and 64 reported seeing a dentist over the past 12 months. Is there a significant difference in use of dental services between children living in Boston and the national data?

We presented the following approach to the test using a Z statistic. 

• Step 1. Set up hypotheses and determine level of significance

H 0 : p = 0.75

H 1 : p ≠ 0.75                               α=0.05

We must first check that the sample size is adequate. Specifically, we need to check min(np 0 , n(1-p 0 )) = min( 125(0.75), 125(1-0.75))=min(94, 31)=31. The sample size is more than adequate so the following formula can be used

This is a two-tailed test, using a Z statistic and a 5% level of significance. Reject H 0 if Z < -1.960 or if Z > 1.960.

We now substitute the sample data into the formula for the test statistic identified in Step 2. The sample proportion is:

We reject H 0 because -6.15 < -1.960. We have statistically significant evidence at a =0.05 to show that there is a statistically significant difference in the use of dental service by children living in Boston as compared to the national data. (p < 0.0001).  

We now conduct the same test using the chi-square goodness-of-fit test. First, we summarize our sample data as follows:

H 0 : p 1 =0.75, p 2 =0.25     or equivalently H 0 : Distribution of responses is 0.75, 0.25 

We must assess whether the sample size is adequate. Specifically, we need to check min(np 0 , np 1, ...,np k >) > 5. The sample size here is n=125 and the proportions specified in the null hypothesis are 0.75, 0.25. Thus, min( 125(0.75), 125(0.25))=min(93.75, 31.25)=31.25. The sample size is more than adequate so the formula can be used.

Here we have df=k-1=2-1=1 and a 5% level of significance. The appropriate critical value is 3.84, and the decision rule is as follows: Reject H 0 if χ 2 > 3.84. (Note that 1.96 2 = 3.84, where 1.96 was the critical value used in the Z test for proportions shown above.)

(Note that (-6.15) 2 = 37.8, where -6.15 was the value of the Z statistic in the test for proportions shown above.)

We reject H 0 because 37.8 > 3.84. We have statistically significant evidence at α=0.05 to show that there is a statistically significant difference in the use of dental service by children living in Boston as compared to the national data.  (p < 0.0001). This is the same conclusion we reached when we conducted the test using the Z test above. With a dichotomous outcome, Z 2 = χ 2 !   In statistics, there are often several approaches that can be used to test hypotheses. 

Tests for Two or More Independent Samples, Discrete Outcome

Here we extend that application of the chi-square test to the case with two or more independent comparison groups. Specifically, the outcome of interest is discrete with two or more responses and the responses can be ordered or unordered (i.e., the outcome can be dichotomous, ordinal or categorical). We now consider the situation where there are two or more independent comparison groups and the goal of the analysis is to compare the distribution of responses to the discrete outcome variable among several independent comparison groups.  

The test is called the χ 2 test of independence and the null hypothesis is that there is no difference in the distribution of responses to the outcome across comparison groups. This is often stated as follows: The outcome variable and the grouping variable (e.g., the comparison treatments or comparison groups) are independent (hence the name of the test). Independence here implies homogeneity in the distribution of the outcome among comparison groups.    

The null hypothesis in the χ 2 test of independence is often stated in words as: H 0 : The distribution of the outcome is independent of the groups. The alternative or research hypothesis is that there is a difference in the distribution of responses to the outcome variable among the comparison groups (i.e., that the distribution of responses "depends" on the group). In order to test the hypothesis, we measure the discrete outcome variable in each participant in each comparison group. The data of interest are the observed frequencies (or number of participants in each response category in each group). The formula for the test statistic for the χ 2 test of independence is given below.

Test Statistic for Testing H 0 : Distribution of outcome is independent of groups

and we find the critical value in a table of probabilities for the chi-square distribution with df=(r-1)*(c-1).

Here O = observed frequency, E=expected frequency in each of the response categories in each group, r = the number of rows in the two-way table and c = the number of columns in the two-way table.   r and c correspond to the number of comparison groups and the number of response options in the outcome (see below for more details). The observed frequencies are the sample data and the expected frequencies are computed as described below. The test statistic is appropriate for large samples, defined as expected frequencies of at least 5 in each of the response categories in each group.  

The data for the χ 2 test of independence are organized in a two-way table. The outcome and grouping variable are shown in the rows and columns of the table. The sample table below illustrates the data layout. The table entries (blank below) are the numbers of participants in each group responding to each response category of the outcome variable.

Table - Possible outcomes are are listed in the columns; The groups being compared are listed in rows.

In the table above, the grouping variable is shown in the rows of the table; r denotes the number of independent groups. The outcome variable is shown in the columns of the table; c denotes the number of response options in the outcome variable. Each combination of a row (group) and column (response) is called a cell of the table. The table has r*c cells and is sometimes called an r x c ("r by c") table. For example, if there are 4 groups and 5 categories in the outcome variable, the data are organized in a 4 X 5 table. The row and column totals are shown along the right-hand margin and the bottom of the table, respectively. The total sample size, N, can be computed by summing the row totals or the column totals. Similar to ANOVA, N does not refer to a population size here but rather to the total sample size in the analysis. The sample data can be organized into a table like the above. The numbers of participants within each group who select each response option are shown in the cells of the table and these are the observed frequencies used in the test statistic.

The test statistic for the χ 2 test of independence involves comparing observed (sample data) and expected frequencies in each cell of the table. The expected frequencies are computed assuming that the null hypothesis is true. The null hypothesis states that the two variables (the grouping variable and the outcome) are independent. The definition of independence is as follows:

 Two events, A and B, are independent if P(A|B) = P(A), or equivalently, if P(A and B) = P(A) P(B).

The second statement indicates that if two events, A and B, are independent then the probability of their intersection can be computed by multiplying the probability of each individual event. To conduct the χ 2 test of independence, we need to compute expected frequencies in each cell of the table. Expected frequencies are computed by assuming that the grouping variable and outcome are independent (i.e., under the null hypothesis). Thus, if the null hypothesis is true, using the definition of independence:

P(Group 1 and Response Option 1) = P(Group 1) P(Response Option 1).

 The above states that the probability that an individual is in Group 1 and their outcome is Response Option 1 is computed by multiplying the probability that person is in Group 1 by the probability that a person is in Response Option 1. To conduct the χ 2 test of independence, we need expected frequencies and not expected probabilities . To convert the above probability to a frequency, we multiply by N. Consider the following small example.

The data shown above are measured in a sample of size N=150. The frequencies in the cells of the table are the observed frequencies. If Group and Response are independent, then we can compute the probability that a person in the sample is in Group 1 and Response category 1 using:

P(Group 1 and Response 1) = P(Group 1) P(Response 1),

P(Group 1 and Response 1) = (25/150) (62/150) = 0.069.

Thus if Group and Response are independent we would expect 6.9% of the sample to be in the top left cell of the table (Group 1 and Response 1). The expected frequency is 150(0.069) = 10.4.   We could do the same for Group 2 and Response 1:

P(Group 2 and Response 1) = P(Group 2) P(Response 1),

P(Group 2 and Response 1) = (50/150) (62/150) = 0.138.

The expected frequency in Group 2 and Response 1 is 150(0.138) = 20.7.

Thus, the formula for determining the expected cell frequencies in the χ 2 test of independence is as follows:

Expected Cell Frequency = (Row Total * Column Total)/N.

The above computes the expected frequency in one step rather than computing the expected probability first and then converting to a frequency.  

In a prior example we evaluated data from a survey of university graduates which assessed, among other things, how frequently they exercised. The survey was completed by 470 graduates. In the prior example we used the χ 2 goodness-of-fit test to assess whether there was a shift in the distribution of responses to the exercise question following the implementation of a health promotion campaign on campus. We specifically considered one sample (all students) and compared the observed distribution to the distribution of responses the prior year (a historical control). Suppose we now wish to assess whether there is a relationship between exercise on campus and students' living arrangements. As part of the same survey, graduates were asked where they lived their senior year. The response options were dormitory, on-campus apartment, off-campus apartment, and at home (i.e., commuted to and from the university). The data are shown below.

Based on the data, is there a relationship between exercise and student's living arrangement? Do you think where a person lives affect their exercise status? Here we have four independent comparison groups (living arrangement) and a discrete (ordinal) outcome variable with three response options. We specifically want to test whether living arrangement and exercise are independent. We will run the test using the five-step approach.  

H 0 : Living arrangement and exercise are independent

H 1 : H 0 is false.                α=0.05

The null and research hypotheses are written in words rather than in symbols. The research hypothesis is that the grouping variable (living arrangement) and the outcome variable (exercise) are dependent or related.   

• Step 2.  Select the appropriate test statistic.  

The condition for appropriate use of the above test statistic is that each expected frequency is at least 5. In Step 4 we will compute the expected frequencies and we will ensure that the condition is met.

The decision rule depends on the level of significance and the degrees of freedom, defined as df = (r-1)(c-1), where r and c are the numbers of rows and columns in the two-way data table.   The row variable is the living arrangement and there are 4 arrangements considered, thus r=4. The column variable is exercise and 3 responses are considered, thus c=3. For this test, df=(4-1)(3-1)=3(2)=6. Again, with χ 2 tests there are no upper, lower or two-tailed tests. If the null hypothesis is true, the observed and expected frequencies will be close in value and the χ 2 statistic will be close to zero. If the null hypothesis is false, then the χ 2 statistic will be large. The rejection region for the χ 2 test of independence is always in the upper (right-hand) tail of the distribution. For df=6 and a 5% level of significance, the appropriate critical value is 12.59 and the decision rule is as follows: Reject H 0 if c 2 > 12.59.

We now compute the expected frequencies using the formula,

Expected Frequency = (Row Total * Column Total)/N.

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency.   The expected frequencies are shown in parentheses.

Notice that the expected frequencies are taken to one decimal place and that the sums of the observed frequencies are equal to the sums of the expected frequencies in each row and column of the table.  

Recall in Step 2 a condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 9.6) and therefore it is appropriate to use the test statistic.

We reject H 0 because 60.5 > 12.59. We have statistically significant evidence at a =0.05 to show that H 0 is false or that living arrangement and exercise are not independent (i.e., they are dependent or related), p < 0.005.  

Again, the χ 2 test of independence is used to test whether the distribution of the outcome variable is similar across the comparison groups. Here we rejected H 0 and concluded that the distribution of exercise is not independent of living arrangement, or that there is a relationship between living arrangement and exercise. The test provides an overall assessment of statistical significance. When the null hypothesis is rejected, it is important to review the sample data to understand the nature of the relationship. Consider again the sample data. 

Because there are different numbers of students in each living situation, it makes the comparisons of exercise patterns difficult on the basis of the frequencies alone. The following table displays the percentages of students in each exercise category by living arrangement. The percentages sum to 100% in each row of the table. For comparison purposes, percentages are also shown for the total sample along the bottom row of the table.

From the above, it is clear that higher percentages of students living in dormitories and in on-campus apartments reported regular exercise (31% and 23%) as compared to students living in off-campus apartments and at home (10% each).  

Test Yourself

 Pancreaticoduodenectomy (PD) is a procedure that is associated with considerable morbidity. A study was recently conducted on 553 patients who had a successful PD between January 2000 and December 2010 to determine whether their Surgical Apgar Score (SAS) is related to 30-day perioperative morbidity and mortality. The table below gives the number of patients experiencing no, minor, or major morbidity by SAS category.  

Question: What would be an appropriate statistical test to examine whether there is an association between Surgical Apgar Score and patient outcome? Using 14.13 as the value of the test statistic for these data, carry out the appropriate test at a 5% level of significance. Show all parts of your test.

In the module on hypothesis testing for means and proportions, we discussed hypothesis testing applications with a dichotomous outcome variable and two independent comparison groups. We presented a test using a test statistic Z to test for equality of independent proportions. The chi-square test of independence can also be used with a dichotomous outcome and the results are mathematically equivalent.  

In the prior module, we considered the following example. Here we show the equivalence to the chi-square test of independence.

A randomized trial is designed to evaluate the effectiveness of a newly developed pain reliever designed to reduce pain in patients following joint replacement surgery. The trial compares the new pain reliever to the pain reliever currently in use (called the standard of care). A total of 100 patients undergoing joint replacement surgery agreed to participate in the trial. Patients were randomly assigned to receive either the new pain reliever or the standard pain reliever following surgery and were blind to the treatment assignment. Before receiving the assigned treatment, patients were asked to rate their pain on a scale of 0-10 with higher scores indicative of more pain. Each patient was then given the assigned treatment and after 30 minutes was again asked to rate their pain on the same scale. The primary outcome was a reduction in pain of 3 or more scale points (defined by clinicians as a clinically meaningful reduction). The following data were observed in the trial.

We tested whether there was a significant difference in the proportions of patients reporting a meaningful reduction (i.e., a reduction of 3 or more scale points) using a Z statistic, as follows. 

H 0 : p 1 = p 2    

H 1 : p 1 ≠ p 2                             α=0.05

Here the new or experimental pain reliever is group 1 and the standard pain reliever is group 2.

We must first check that the sample size is adequate. Specifically, we need to ensure that we have at least 5 successes and 5 failures in each comparison group or that:

In this example, we have

Therefore, the sample size is adequate, so the following formula can be used:

Reject H 0 if Z < -1.960 or if Z > 1.960.

We now substitute the sample data into the formula for the test statistic identified in Step 2. We first compute the overall proportion of successes:

We now substitute to compute the test statistic.

• Step 5.  Conclusion.  

We now conduct the same test using the chi-square test of independence.  

H 0 : Treatment and outcome (meaningful reduction in pain) are independent

H 1 :   H 0 is false.         α=0.05

The formula for the test statistic is:  

For this test, df=(2-1)(2-1)=1. At a 5% level of significance, the appropriate critical value is 3.84 and the decision rule is as follows: Reject H0 if χ 2 > 3.84. (Note that 1.96 2 = 3.84, where 1.96 was the critical value used in the Z test for proportions shown above.)

We now compute the expected frequencies using:

The computations can be organized in a two-way table. The top number in each cell of the table is the observed frequency and the bottom number is the expected frequency. The expected frequencies are shown in parentheses.

A condition for the appropriate use of the test statistic was that each expected frequency is at least 5. This is true for this sample (the smallest expected frequency is 22.0) and therefore it is appropriate to use the test statistic.

(Note that (2.53) 2 = 6.4, where 2.53 was the value of the Z statistic in the test for proportions shown above.)

Chi-Squared Tests in R

The video below by Mike Marin demonstrates how to perform chi-squared tests in the R programming language.

Answer to Problem on Pancreaticoduodenectomy and Surgical Apgar Scores

We have 3 independent comparison groups (Surgical Apgar Score) and a categorical outcome variable (morbidity/mortality). We can run a Chi-Squared test of independence.

H 0 : Apgar scores and patient outcome are independent of one another.

H A : Apgar scores and patient outcome are not independent.

Chi-squared = 14.3

Since 14.3 is greater than 9.49, we reject H 0.

There is an association between Apgar scores and patient outcome. The lowest Apgar score group (0 to 4) experienced the highest percentage of major morbidity or mortality (16 out of 57=28%) compared to the other Apgar score groups.

11.3 - Chi-Square Test of Independence

The chi-square (\(\chi^2\)) test of independence is used to test for a relationship between two categorical variables. Recall that if two categorical variables are independent, then \(P(A) = P(A \mid B)\). The chi-square test of independence uses this fact to compute expected values for the cells in a two-way contingency table under the assumption that the two variables are independent (i.e., the null hypothesis is true).

Even if two variables are independent in the population, samples will vary due to random sampling variation. The chi-square test is used to determine if there is evidence that the two variables are not independent in the population using the same hypothesis testing logic that we used with one mean, one proportion, etc.

Again, we will be using the five step hypothesis testing procedure:

The assumptions are that the sample is randomly drawn from the population and that all expected values are at least 5 (we will see what expected values are later).

Our hypotheses are:

     \(H_0:\) There is not a relationship between the two variables in the population (they are independent)

     \(H_a:\) There is a relationship between the two variables in the population (they are dependent)

Note: When you're writing the hypotheses for a given scenario, use the names of the variables, not the generic "two variables."

The p-value can be found using Minitab. Look up the area to the right of your chi-square test statistic on a chi-square distribution with the correct degrees of freedom. Chi-square tests are always right-tailed tests. 

If \(p \leq \alpha\) reject the null hypothesis.

If \(p>\alpha\) fail to reject the null hypothesis.

Write a conclusion in terms of the original research question.

11.3.1 - Example: Gender and Online Learning

Gender and online learning.

A sample of 314 Penn State students was asked if they have ever taken an online course. Their genders were also recorded. The contingency table below was constructed. Use a chi-square test of independence to determine if there is a relationship between gender and whether or not someone has taken an online course.

\(H_0:\) There is not a relationship between gender and whether or not someone has taken an online course (they are independent)

\(H_a:\) There is a relationship between gender and whether or not someone has taken an online course (they are dependent)

Looking ahead to our calculations of the expected values, we can see that all expected values are at least 5. This means that the sampling distribution can be approximated using the \(\chi^2\) distribution. 

In order to compute the chi-square test statistic we must know the observed and expected values for each cell. We are given the observed values in the table above. We must compute the expected values. The table below includes the row and column totals.

Note that all expected values are at least 5, thus this assumption of the \(\chi^2\) test of independence has been met. 

Observed and expected counts are often presented together in a contingency table. In the table below, expected values are presented in parentheses.

\(\chi^2=\sum \dfrac{(O-E)^2}{E} \)

\(\chi^2=\dfrac{(43-46.586)^2}{46.586}+\dfrac{(63-59.414)^2}{59.414}+\dfrac{(95-91.414)^2}{91.414}+\dfrac{(113-116.586)^2}{116.586}=0.276+0.216+0.141+0.110=0.743\)

The chi-square test statistic is 0.743

\(df=(number\;of\;rows-1)(number\;of\;columns-1)=(2-1)(2-1)=1\)

We can determine the p-value by constructing a chi-square distribution plot with 1 degree of freedom and finding the area to the right of 0.743.

\(p = 0.388702\)

\(p>\alpha\), therefore we fail to reject the null hypothesis.

There is not enough evidence to conclude that gender and whether or not an individual has completed an online course are related.

Note that we cannot say for sure that these two categorical variables are independent, we can only say that we do not have enough evidence that they are dependent.

11.3.2 - Minitab: Test of Independence

Raw vs summarized data.

If you have a data file with the responses for individual cases then you have "raw data" and can follow the directions below. If you have a table filled with data, then you have "summarized data." There is an example of conducting a chi-square test of independence using summarized data on a later page. After data entry the procedure is the same for both data entry methods.

Minitab ®  – Chi-square Test Using Raw Data

Research question : Is there a relationship between where a student sits in class and whether they have ever cheated?

• Null hypothesis : Seat location and cheating are not related in the population. 
• Alternative hypothesis : Seat location and cheating are related in the population.

To perform a chi-square test of independence in Minitab using raw data:

• Open Minitab file: class_survey.mpx
• Select Stat > Tables > Chi-Square Test for Association
• Select Raw data (categorical variables) from the dropdown.
• Choose the variable  Seating  to insert it into the  Rows  box
• Choose the variable  Ever_Cheat  to insert it into the  Columns  box
• Click the Statistics button and check the boxes  Chi-square test for association  and  Expected cell counts
• Click  OK and OK

This should result in the following output:

Rows: Seating Columns: Ever_Cheat

Chi-square test.

All expected values are at least 5 so we can use the Pearson chi-square test statistic. Our results are \(\chi^2 (2) = 1.539\). \(p = 0.463\). Because our \(p\) value is greater than the standard alpha level of 0.05, we fail to reject the null hypothesis. There is not enough evidence of a relationship in the population between seat location and whether a student has cheated.

11.3.2.1 - Example: Raw Data

Example: dog & cat ownership.

Is there a relationship between dog and cat ownership in the population of all World Campus STAT 200 students? Let's conduct an hypothesis test using the dataset: fall2016stdata.mpx

 \(H_0:\) There is not a relationship between dog ownership and cat ownership in the population of all World Campus STAT 200 students \(H_a:\) There is a relationship between dog ownership and cat ownership in the population of all World Campus STAT 200 students

Assumption: All expected counts are at least 5. The expected counts here are 176.02, 75.98, 189.98, and 82.02, so this assumption has been met.

Let's use Minitab to calculate the test statistic and p-value.

• After entering the data, select Stat > Tables > Cross Tabulation and Chi-Square
• Enter Dog in the Rows box
• Enter Cat in the Columns box
• Select the Chi-Square button and in the new window check the box for the Chi-square test and Expected cell counts

Rows: Dog Columns: Cat

Since the assumption was met in step 1, we can use the Pearson chi-square test statistic.

\(Pearson\;\chi^2 = 1.771\)

\(p = 0.183\)

Our p value is greater than the standard 0.05 alpha level, so we fail to reject the null hypothesis.

There is not enough evidence of a relationship between dog ownership and cat ownership in the population of all World Campus STAT 200 students.

11.3.2.2 - Example: Summarized Data

Example: coffee and tea preference.

Is there a relationship between liking tea and liking coffee?

The following table shows data collected from a random sample of 100 adults. Each were asked if they liked coffee (yes or no) and if they liked tea (yes or no).

Let's use the 5 step hypothesis testing procedure to address this research question.

 \(H_0:\) Liking coffee an liking tea are not related (i.e., independent) in the population \(H_a:\) Liking coffee and liking tea are related (i.e., dependent) in the population

Assumption: All expected counts are at least 5.

• Select Stat > Tables > Cross Tabulation and Chi-Square
• Select Summarized data in a two-way table from the dropdown
• Enter the columns Likes Coffee-Yes and Likes Coffee-No in the Columns containing the table box
• For the row labels enter Likes Tea (leave the column labels blank)
• Select the Chi-Square button and check the boxes for Chi-square test and Expected cell counts .

Rows: Likes Tea  Columns: Worksheet columns

\(Pearson\;\chi^2 = 10.774\)

\(p = 0.001\)

Our p value is less than the standard 0.05 alpha level, so we reject the null hypothesis.

There is evidence of a relationship between between liking coffee and liking tea in the population.

11.3.3 - Relative Risk

A chi-square test of independence will give you information concerning whether or not a relationship between two categorical variables in the population is likely. As was the case with the single sample and two sample hypothesis tests that you learned earlier this semester, with a large sample size statistical power is high and the probability of rejecting the null hypothesis is high, even if the relationship is relatively weak. In addition to examining statistical significance by looking at the p value, we can also examine practical significance by computing the  relative risk .

In Lesson 2 you learned that risk is often used to describe the probability of an event occurring. Risk can also be used to compare the probabilities in two different groups. First, we'll review risk, then you'll be introduced to the concept of relative risk.

The  risk  of an outcome can be expressed as a fraction or as the percent of a group that experiences the outcome.

Examples of Risk

60 out of 1000 teens have asthma. The risk is \(\frac{60}{1000}=.06\). This means that 6% of all teens experience asthma.

45 out of 100 children get the flu each year. The risk is \(\frac{45}{100}=.45\) or 45%

Thus, relative risk gives the risk for group 1 as a multiple of the risk for group 2.

Example of Relative Risk

Suppose that the risk of a child getting the flu this year is .45 and the risk of an adult getting the flu this year is .10. What is the relative risk of children compared to adults?

• \(Relative\;risk=\dfrac{.45}{.10}=4.5\)

Children are 4.5 times more likely than adults to get the flu this year.

Watch out for relative risk statistics where no baseline information is given about the actual risk. For instance, it doesn't mean much to say that beer drinkers have twice the risk of stomach cancer as non-drinkers unless we know the actual risks. The risk of stomach cancer might actually be very low, even for beer drinkers. For example, 2 in a million is twice the size of 1 in a million but is would still be a very low risk. This is known as the  baseline  with which other risks are compared.

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• SPSS Tutorials

Chi-Square Test of Independence

Spss tutorials: chi-square test of independence.

• The SPSS Environment
• The Data View Window
• Using SPSS Syntax
• Data Creation in SPSS
• Importing Data into SPSS
• Variable Types
• Date-Time Variables in SPSS
• Defining Variables
• Creating a Codebook
• Computing Variables
• Computing Variables: Mean Centering
• Computing Variables: Recoding Categorical Variables
• Computing Variables: Recoding String Variables into Coded Categories (Automatic Recode)
• rank transform converts a set of data values by ordering them from smallest to largest, and then assigning a rank to each value. In SPSS, the Rank Cases procedure can be used to compute the rank transform of a variable." href="https://libguides.library.kent.edu/SPSS/RankCases" style="" >Computing Variables: Rank Transforms (Rank Cases)
• Weighting Cases
• Sorting Data
• Grouping Data
• Descriptive Stats for One Numeric Variable (Explore)
• Descriptive Stats for One Numeric Variable (Frequencies)
• Descriptive Stats for Many Numeric Variables (Descriptives)
• Descriptive Stats by Group (Compare Means)
• Frequency Tables
• Working with "Check All That Apply" Survey Data (Multiple Response Sets)
• Pearson Correlation
• One Sample t Test
• Paired Samples t Test
• Independent Samples t Test
• One-Way ANOVA
• How to Cite the Tutorials

Sample Data Files

Our tutorials reference a dataset called "sample" in many examples. If you'd like to download the sample dataset to work through the examples, choose one of the files below:

• Data definitions (*.pdf)
• Data - Comma delimited (*.csv)
• Data - Tab delimited (*.txt)
• Data - Excel format (*.xlsx)
• Data - SAS format (*.sas7bdat)
• Data - SPSS format (*.sav)
• SPSS Syntax (*.sps) Syntax to add variable labels, value labels, set variable types, and compute several recoded variables used in later tutorials.
• SAS Syntax (*.sas) Syntax to read the CSV-format sample data and set variable labels and formats/value labels.

The Chi-Square Test of Independence determines whether there is an association between categorical variables (i.e., whether the variables are independent or related). It is a nonparametric test.

This test is also known as:

• Chi-Square Test of Association.

This test utilizes a contingency table to analyze the data. A contingency table (also known as a cross-tabulation , crosstab , or two-way table ) is an arrangement in which data is classified according to two categorical variables. The categories for one variable appear in the rows, and the categories for the other variable appear in columns. Each variable must have two or more categories. Each cell reflects the total count of cases for a specific pair of categories.

There are several tests that go by the name "chi-square test" in addition to the Chi-Square Test of Independence. Look for context clues in the data and research question to make sure what form of the chi-square test is being used.

Common Uses

The Chi-Square Test of Independence is commonly used to test the following:

• Statistical independence or association between two categorical variables.

The Chi-Square Test of Independence can only compare categorical variables. It cannot make comparisons between continuous variables or between categorical and continuous variables. Additionally, the Chi-Square Test of Independence only assesses associations between categorical variables, and can not provide any inferences about causation.

If your categorical variables represent "pre-test" and "post-test" observations, then the chi-square test of independence is not appropriate . This is because the assumption of the independence of observations is violated. In this situation, McNemar's Test is appropriate.

Data Requirements

Your data must meet the following requirements:

• Two categorical variables.
• Two or more categories (groups) for each variable.
• There is no relationship between the subjects in each group.
• The categorical variables are not "paired" in any way (e.g. pre-test/post-test observations).
• Expected frequencies for each cell are at least 1.
• Expected frequencies should be at least 5 for the majority (80%) of the cells.

The null hypothesis ( H 0 ) and alternative hypothesis ( H 1 ) of the Chi-Square Test of Independence can be expressed in two different but equivalent ways:

H 0 : "[ Variable 1 ] is independent of [ Variable 2 ]" H 1 : "[ Variable 1 ] is not independent of [ Variable 2 ]"

H 0 : "[ Variable 1 ] is not associated with [ Variable 2 ]" H 1 :  "[ Variable 1 ] is associated with [ Variable 2 ]"

Test Statistic

The test statistic for the Chi-Square Test of Independence is denoted Χ 2 , and is computed as:

$$ \chi^{2} = \sum_{i=1}^{R}{\sum_{j=1}^{C}{\frac{(o_{ij} - e_{ij})^{2}}{e_{ij}}}} $$

\(o_{ij}\) is the observed cell count in the i th row and j th column of the table

\(e_{ij}\) is the expected cell count in the i th row and j th column of the table, computed as

$$ e_{ij} = \frac{\mathrm{ \textrm{row } \mathit{i}} \textrm{ total} * \mathrm{\textrm{col } \mathit{j}} \textrm{ total}}{\textrm{grand total}} $$

The quantity ( o ij - e ij ) is sometimes referred to as the residual of cell ( i , j ), denoted \(r_{ij}\).

The calculated Χ 2 value is then compared to the critical value from the Χ 2 distribution table with degrees of freedom df = ( R - 1)( C - 1) and chosen confidence level. If the calculated Χ 2 value > critical Χ 2 value, then we reject the null hypothesis.

Data Set-Up

There are two different ways in which your data may be set up initially. The format of the data will determine how to proceed with running the Chi-Square Test of Independence. At minimum, your data should include two categorical variables (represented in columns) that will be used in the analysis. The categorical variables must include at least two groups. Your data may be formatted in either of the following ways:

If you have the raw data (each row is a subject):

• Cases represent subjects, and each subject appears once in the dataset. That is, each row represents an observation from a unique subject.
• The dataset contains at least two nominal categorical variables (string or numeric). The categorical variables used in the test must have two or more categories.

If you have frequencies (each row is a combination of factors):

An example of using the chi-square test for this type of data can be found in the Weighting Cases tutorial .

• Each row in the dataset represents a distinct combination of the categories.
• The value in the "frequency" column for a given row is the number of unique subjects with that combination of categories.
• You should have three variables: one representing each category, and a third representing the number of occurrences of that particular combination of factors.
• Before running the test, you must activate Weight Cases, and set the frequency variable as the weight.

Run a Chi-Square Test of Independence

In SPSS, the Chi-Square Test of Independence is an option within the Crosstabs procedure. Recall that the Crosstabs procedure creates a contingency table or two-way table , which summarizes the distribution of two categorical variables.

To create a crosstab and perform a chi-square test of independence, click  Analyze > Descriptive Statistics > Crosstabs .

A Row(s): One or more variables to use in the rows of the crosstab(s). You must enter at least one Row variable.

B Column(s): One or more variables to use in the columns of the crosstab(s). You must enter at least one Column variable.

Also note that if you specify one row variable and two or more column variables, SPSS will print crosstabs for each pairing of the row variable with the column variables. The same is true if you have one column variable and two or more row variables, or if you have multiple row and column variables. A chi-square test will be produced for each table. Additionally, if you include a layer variable, chi-square tests will be run for each pair of row and column variables within each level of the layer variable.

C Layer: An optional "stratification" variable. If you have turned on the chi-square test results and have specified a layer variable, SPSS will subset the data with respect to the categories of the layer variable, then run chi-square tests between the row and column variables. (This is not equivalent to testing for a three-way association, or testing for an association between the row and column variable after controlling for the layer variable.)

D Statistics: Opens the Crosstabs: Statistics window, which contains fifteen different inferential statistics for comparing categorical variables.

To run the Chi-Square Test of Independence, make sure that the Chi-square box is checked.

E Cells: Opens the Crosstabs: Cell Display window, which controls which output is displayed in each cell of the crosstab. (Note: in a crosstab, the cells are the inner sections of the table. They show the number of observations for a given combination of the row and column categories.) There are three options in this window that are useful (but optional) when performing a Chi-Square Test of Independence:

1 Observed : The actual number of observations for a given cell. This option is enabled by default.

2 Expected : The expected number of observations for that cell (see the test statistic formula).

3 Unstandardized Residuals : The "residual" value, computed as observed minus expected.

F Format: Opens the Crosstabs: Table Format window, which specifies how the rows of the table are sorted.

Example: Chi-square Test for 3x2 Table

Problem statement.

In the sample dataset, respondents were asked their gender and whether or not they were a cigarette smoker. There were three answer choices: Nonsmoker, Past smoker, and Current smoker. Suppose we want to test for an association between smoking behavior (nonsmoker, current smoker, or past smoker) and gender (male or female) using a Chi-Square Test of Independence (we'll use α = 0.05).

Before the Test

Before we test for "association", it is helpful to understand what an "association" and a "lack of association" between two categorical variables looks like. One way to visualize this is using clustered bar charts. Let's look at the clustered bar chart produced by the Crosstabs procedure.

This is the chart that is produced if you use Smoking as the row variable and Gender as the column variable (running the syntax later in this example):

The "clusters" in a clustered bar chart are determined by the row variable (in this case, the smoking categories). The color of the bars is determined by the column variable (in this case, gender). The height of each bar represents the total number of observations in that particular combination of categories.

This type of chart emphasizes the differences within the categories of the row variable. Notice how within each smoking category, the heights of the bars (i.e., the number of males and females) are very similar. That is, there are an approximately equal number of male and female nonsmokers; approximately equal number of male and female past smokers; approximately equal number of male and female current smokers. If there were an association between gender and smoking, we would expect these counts to differ between groups in some way.

Running the Test

• Open the Crosstabs dialog ( Analyze > Descriptive Statistics > Crosstabs ).
• Select Smoking as the row variable, and Gender as the column variable.
• Click Statistics . Check Chi-square , then click Continue .
• (Optional) Check the box for Display clustered bar charts .

The first table is the Case Processing summary, which tells us the number of valid cases used for analysis. Only cases with nonmissing values for both smoking behavior and gender can be used in the test.

The next tables are the crosstabulation and chi-square test results.

The key result in the Chi-Square Tests table is the Pearson Chi-Square.

• The value of the test statistic is 3.171.
• The footnote for this statistic pertains to the expected cell count assumption (i.e., expected cell counts are all greater than 5): no cells had an expected count less than 5, so this assumption was met.
• Because the test statistic is based on a 3x2 crosstabulation table, the degrees of freedom (df) for the test statistic is $$ df = (R - 1)*(C - 1) = (3 - 1)*(2 - 1) = 2*1 = 2 $$.
• The corresponding p-value of the test statistic is p = 0.205.

Decision and Conclusions

Since the p-value is greater than our chosen significance level ( α = 0.05), we do not reject the null hypothesis. Rather, we conclude that there is not enough evidence to suggest an association between gender and smoking.

Based on the results, we can state the following:

• No association was found between gender and smoking behavior ( Χ 2 (2)> = 3.171, p = 0.205).

Example: Chi-square Test for 2x2 Table

Let's continue the row and column percentage example from the Crosstabs tutorial, which described the relationship between the variables RankUpperUnder (upperclassman/underclassman) and LivesOnCampus (lives on campus/lives off-campus). Recall that the column percentages of the crosstab appeared to indicate that upperclassmen were less likely than underclassmen to live on campus:

• The proportion of underclassmen who live off campus is 34.8%, or 79/227.
• The proportion of underclassmen who live on campus is 65.2%, or 148/227.
• The proportion of upperclassmen who live off campus is 94.4%, or 152/161.
• The proportion of upperclassmen who live on campus is 5.6%, or 9/161.

Suppose that we want to test the association between class rank and living on campus using a Chi-Square Test of Independence (using α = 0.05).

The clustered bar chart from the Crosstabs procedure can act as a complement to the column percentages above. Let's look at the chart produced by the Crosstabs procedure for this example:

The height of each bar represents the total number of observations in that particular combination of categories. The "clusters" are formed by the row variable (in this case, class rank). This type of chart emphasizes the differences within the underclassmen and upperclassmen groups. Here, the differences in number of students living on campus versus living off-campus is much starker within the class rank groups.

• Select RankUpperUnder as the row variable, and LiveOnCampus as the column variable.
• (Optional) Click Cells . Under Counts, check the boxes for Observed and Expected , and under Residuals, click Unstandardized . Then click Continue .

The first table is the Case Processing summary, which tells us the number of valid cases used for analysis. Only cases with nonmissing values for both class rank and living on campus can be used in the test.

The next table is the crosstabulation. If you elected to check off the boxes for Observed Count, Expected Count, and Unstandardized Residuals, you should see the following table:

With the Expected Count values shown, we can confirm that all cells have an expected value greater than 5.

These numbers can be plugged into the chi-square test statistic formula:

$$ \chi^{2} = \sum_{i=1}^{R}{\sum_{j=1}^{C}{\frac{(o_{ij} - e_{ij})^{2}}{e_{ij}}}} = \frac{(-56.147)^{2}}{135.147} + \frac{(56.147)^{2}}{91.853} + \frac{(56.147)^{2}}{95.853} + \frac{(-56.147)^{2}}{65.147} = 138.926 $$

We can confirm this computation with the results in the Chi-Square Tests table:

The row of interest here is Pearson Chi-Square and its footnote.

• The value of the test statistic is 138.926.
• Because the crosstabulation is a 2x2 table, the degrees of freedom (df) for the test statistic is $$ df = (R - 1)*(C - 1) = (2 - 1)*(2 - 1) = 1 $$.
• The corresponding p-value of the test statistic is so small that it is cut off from display. Instead of writing "p = 0.000", we instead write the mathematically correct statement p < 0.001.

Since the p-value is less than our chosen significance level α = 0.05, we can reject the null hypothesis, and conclude that there is an association between class rank and whether or not students live on-campus.

• There was a significant association between class rank and living on campus ( Χ 2 (1) = 138.9, p < .001).
• << Previous: Analyzing Data
• Next: Pearson Correlation >>
• Last Updated: May 10, 2024 1:32 PM
• URL: https://libguides.library.kent.edu/SPSS

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Statistics Made Easy

Chi-Square Goodness of Fit Test: Definition, Formula, and Example

A  Chi-Square goodness of fit test  is used to determine whether or not a categorical variable follows a hypothesized distribution.

This tutorial explains the following:

• The motivation for performing a Chi-Square goodness of fit test.
• The formula to perform a Chi-Square goodness of fit test.
• An example of how to perform a Chi-Square goodness of fit test.

Chi-Square Goodness of Fit Test: Motivation

A Chi-Square goodness of fit test can be used in a wide variety of settings. Here are a few examples:

• We want to know if a die is fair, so we roll it 50 times and record the number of times it lands on each number.
• We want to know if an equal number of people come into a shop each day of the week, so we count the number of people who come in each day during a random week.
• We want to know if the percentage of M&M’s that come in a bag are as follows: 20% yellow, 30% blue, 30% red, 20% other. To test this, we open a random bag of M&M’s and count how many of each color appear.

In each of these scenarios, we want to know if some variable follows a hypothesized distribution. In each scenario, we can use a Chi-Square goodness of fit test to determine if there is a statistically significant difference in the number of expected counts for each level of a variable compared to the observed counts.

Chi-Square Goodness of Fit Test: Formula

A Chi-Square goodness of fit test uses the following null and alternative hypotheses:

• H 0 : (null hypothesis)  A variable follows a hypothesized distribution.
• H 1 : (alternative hypothesis)  A variable does not follow a hypothesized distribution.

We use the following formula to calculate the Chi-Square test statistic X 2 :

X 2 = Σ(O-E) 2  / E

• Σ:  is a fancy symbol that means “sum”
• O:  observed value
• E:  expected value

If the p-value that corresponds to the test statistic X 2  with n-1 degrees of freedom (where n is the number of categories) is less than your chosen significance level (common choices are 0.10, 0.05, and 0.01) then you can reject the null hypothesis.

Chi-Square Goodness of Fit Test: Example

A shop owner claims that an equal number of customers come into his shop each weekday. To test this hypothesis, an independent researcher records the number of customers that come into the shop on a given week and finds the following:

• Monday:  50 customers
• Tuesday:  60 customers
• Wednesday:  40 customers
• Thursday:  47 customers
• Friday:  53 customers

We will use the following steps to perform a Chi-Square goodness of fit test to determine if the data is consistent with the shop owner’s claim.

Step 1: Define the hypotheses.

We will perform the Chi-Square goodness of fit test using the following hypotheses:

• H 0 :  An equal number of customers come into the shop each day.
• H 1 :  An equal number of customers do not come into the shop each day.

Step 2: Calculate (O-E) 2  / E for each day.

There were a total of 250 customers that came into the shop during the week. Thus, if we expected an equal amount to come in each day then the expected value “E” for each day would be 50.

• Monday:  (50-50) 2  / 50 = 0
• Tuesday:  (60-50) 2  / 50 = 2
• Wednesday:  (40-50) 2  / 50 = 2
• Thursday:  (47-50) 2  / 50 = 0.18
• Friday:  (53-50) 2  / 50 = 0.18

Step 3: Calculate the test statistic X 2 .

X 2  = Σ(O-E) 2  / E = 0 + 2 + 2 + 0.18 + 0.18 =  4.36

Step 4: Calculate the p-value of the test statistic X 2 .

According to the Chi-Square Score to P Value Calculator , the p-value associated with X 2  = 4.36 and n-1 = 5-1 = 4 degrees of freedom is  0.359472 .

Step 5: Draw a conclusion.

Since this p-value is not less than 0.05, we fail to reject the null hypothesis. This means we do not have sufficient evidence to say that the true distribution of customers is different from the distribution that the shop owner claimed.

Note:  You can also perform this entire test by simply using the Chi-Square Goodness of Fit Test Calculator .

Additional Resources

The following tutorials explain how to perform a Chi-Square goodness of fit test using different statistical programs:

How to Perform a Chi-Square Goodness of Fit Test in Excel How to Perform a Chi-Square Goodness of Fit Test in Stata How to Perform a Chi-Square Goodness of Fit Test in SPSS How to Perform a Chi-Square Goodness of Fit Test in Python How to Perform a Chi-Square Goodness of Fit Test in R Chi-Square Goodness of Fit Test on a TI-84 Calculator Chi-Square Goodness of Fit Test Calculator

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Hey there. My name is Zach Bobbitt. I have a Masters of Science degree in Applied Statistics and I’ve worked on machine learning algorithms for professional businesses in both healthcare and retail. I’m passionate about statistics, machine learning, and data visualization and I created Statology to be a resource for both students and teachers alike.  My goal with this site is to help you learn statistics through using simple terms, plenty of real-world examples, and helpful illustrations.

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A test of a single variance assumes that the underlying distribution is normal . The null and alternative hypotheses are stated in terms of the population variance (or population standard deviation). The test statistic is:

\[\chi^{2} = \frac{(n-1)s^{2}}{\sigma^{2}} \label{test}\]

• \(n\) is the the total number of data
• \(s^{2}\) is the sample variance
• \(\sigma^{2}\) is the population variance

You may think of \(s\) as the random variable in this test. The number of degrees of freedom is \(df = n - 1\). A test of a single variance may be right-tailed, left-tailed, or two-tailed. The next example will show you how to set up the null and alternative hypotheses. The null and alternative hypotheses contain statements about the population variance.

Example \(\PageIndex{1}\)

Math instructors are not only interested in how their students do on exams, on average, but how the exam scores vary. To many instructors, the variance (or standard deviation) may be more important than the average.

Suppose a math instructor believes that the standard deviation for his final exam is five points. One of his best students thinks otherwise. The student claims that the standard deviation is more than five points. If the student were to conduct a hypothesis test, what would the null and alternative hypotheses be?

Even though we are given the population standard deviation, we can set up the test using the population variance as follows.

• \(H_{0}: \sigma^{2} = 5^{2}\)
• \(H_{a}: \sigma^{2} > 5^{2}\)

Exercise \(\PageIndex{1}\)

A SCUBA instructor wants to record the collective depths each of his students dives during their checkout. He is interested in how the depths vary, even though everyone should have been at the same depth. He believes the standard deviation is three feet. His assistant thinks the standard deviation is less than three feet. If the instructor were to conduct a test, what would the null and alternative hypotheses be?

• \(H_{0}: \sigma^{2} = 3^{2}\)
• \(H_{a}: \sigma^{2} > 3^{2}\)

Example \(\PageIndex{2}\)

With individual lines at its various windows, a post office finds that the standard deviation for normally distributed waiting times for customers on Friday afternoon is 7.2 minutes. The post office experiments with a single, main waiting line and finds that for a random sample of 25 customers, the waiting times for customers have a standard deviation of 3.5 minutes.

With a significance level of 5%, test the claim that a single line causes lower variation among waiting times (shorter waiting times) for customers .

Since the claim is that a single line causes less variation, this is a test of a single variance. The parameter is the population variance, \(\sigma^{2}\), or the population standard deviation, \(\sigma\).

Random Variable: The sample standard deviation, \(s\), is the random variable. Let \(s = \text{standard deviation for the waiting times}\).

• \(H_{0}: \sigma^{2} = 7.2^{2}\)
• \(H_{a}: \sigma^{2} < 7.2^{2}\)

The word "less" tells you this is a left-tailed test.

Distribution for the test: \(\chi^{2}_{24}\), where:

• \(n = \text{the number of customers sampled}\)
• \(df = n - 1 = 25 - 1 = 24\)

Calculate the test statistic (Equation \ref{test}):

\[\chi^{2} = \frac{(n-1)s^{2}}{\sigma^{2}} = \frac{(25-1)(3.5)^{2}}{7.2^{2}} = 5.67 \nonumber\]

where \(n = 25\), \(s = 3.5\), and \(\sigma = 7.2\).

Probability statement: \(p\text{-value} = P(\chi^{2} < 5.67) = 0.000042\)

Compare \(\alpha\) and the \(p\text{-value}\) :

\[\alpha = 0.05 (p\text{-value} = 0.000042 \alpha > p\text{-value} \nonumber\]

Make a decision: Since \(\alpha > p\text{-value}\), reject \(H_{0}\). This means that you reject \(\sigma^{2} = 7.2^{2}\). In other words, you do not think the variation in waiting times is 7.2 minutes; you think the variation in waiting times is less.

Conclusion: At a 5% level of significance, from the data, there is sufficient evidence to conclude that a single line causes a lower variation among the waiting times or with a single line, the customer waiting times vary less than 7.2 minutes.

In 2nd DISTR , use 7:χ2cdf . The syntax is (lower, upper, df) for the parameter list. For Example , χ2cdf(-1E99,5.67,24) . The \(p\text{-value} = 0.000042\).

Exercise \(\PageIndex{2}\)

The FCC conducts broadband speed tests to measure how much data per second passes between a consumer’s computer and the internet. As of August of 2012, the standard deviation of Internet speeds across Internet Service Providers (ISPs) was 12.2 percent. Suppose a sample of 15 ISPs is taken, and the standard deviation is 13.2. An analyst claims that the standard deviation of speeds is more than what was reported. State the null and alternative hypotheses, compute the degrees of freedom, the test statistic, sketch the graph of the p -value, and draw a conclusion. Test at the 1% significance level.

• \(H_{0}: \sigma^{2} = 12.2^{2}\)
• \(H_{a}: \sigma^{2} > 12.2^{2}\)

In 2nd DISTR , use7: χ2cdf . The syntax is (lower, upper, df) for the parameter list. χ2cdf(16.39,10^99,14) . The \(p\text{-value} = 0.2902\).

\(df = 14\)

\[\text{chi}^{2} \text{test statistic} = 16.39 \nonumber\]

The \(p\text{-value}\) is \(0.2902\), so we decline to reject the null hypothesis. There is not enough evidence to suggest that the variance is greater than \(12.2^{2}\).

• “AppleInsider Price Guides.” Apple Insider, 2013. Available online at http://appleinsider.com/mac_price_guide (accessed May 14, 2013).
• Data from the World Bank, June 5, 2012.

To test variability, use the chi-square test of a single variance. The test may be left-, right-, or two-tailed, and its hypotheses are always expressed in terms of the variance (or standard deviation).

Formula Review

\(\chi^{2} = \frac{(n-1) \cdot s^{2}}{\sigma^{2}}\) Test of a single variance statistic where:

\(n: \text{sample size}\)

\(s: \text{sample standard deviation}\)

\(\sigma: \text{population standard deviation}\)

\(df = n – 1 \text{Degrees of freedom}\)

Test of a Single Variance

• Use the test to determine variation.
• The degrees of freedom is the \(\text{number of samples} - 1\).
• The test statistic is \(\frac{(n-1) \cdot s^{2}}{\sigma^{2}}\), where \(n = \text{the total number of data}\), \(s^{2} = \text{sample variance}\), and \(\sigma^{2} = \text{population variance}\).
• The test may be left-, right-, or two-tailed.

Use the following information to answer the next three exercises: An archer’s standard deviation for his hits is six (data is measured in distance from the center of the target). An observer claims the standard deviation is less.

Exercise \(\PageIndex{3}\)

What type of test should be used?

a test of a single variance

Exercise \(\PageIndex{4}\)

State the null and alternative hypotheses.

Exercise \(\PageIndex{5}\)

Is this a right-tailed, left-tailed, or two-tailed test?

a left-tailed test

Use the following information to answer the next three exercises: The standard deviation of heights for students in a school is 0.81. A random sample of 50 students is taken, and the standard deviation of heights of the sample is 0.96. A researcher in charge of the study believes the standard deviation of heights for the school is greater than 0.81.

Exercise \(\PageIndex{6}\)

\(H_{0}: \sigma^{2} = 0.81^{2}\);

\(H_{a}: \sigma^{2} > 0.81^{2}\)

\(df =\) ________

Use the following information to answer the next four exercises: The average waiting time in a doctor’s office varies. The standard deviation of waiting times in a doctor’s office is 3.4 minutes. A random sample of 30 patients in the doctor’s office has a standard deviation of waiting times of 4.1 minutes. One doctor believes the variance of waiting times is greater than originally thought.

Exercise \(\PageIndex{7}\)

Exercise \(\pageindex{8}\).

What is the test statistic?

Exercise \(\PageIndex{9}\)

What is the \(p\text{-value}\)?

Exercise \(\PageIndex{10}\)

What can you conclude at the 5% significance level?

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Understanding the Null Hypothesis in Chi-Square

The null hypothesis in chi square testing suggests no significant difference between a study’s observed and expected frequencies. It assumes any observed difference is due to chance and not because of a meaningful statistical relationship.

Introduction

The chi-square test is a valuable tool in statistical analysis. It’s a non-parametric test applied when the data are qualitative or categorical. This test helps to establish whether there is a significant association between 2 categorical variables in a sample population.

Central to any chi-square test is the concept of the null hypothesis. In the context of chi-square, the null hypothesis assumes no significant difference exists between the categories’ observed and expected frequencies. Any difference seen is likely due to chance or random error rather than a meaningful statistical difference.

• The chi-square null hypothesis assumes no significant difference between observed and expected frequencies.
• Failing to reject the null hypothesis doesn’t prove it true, only that data lacks strong evidence against it.
• A p-value < the significance level indicates a significant association between variables.

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Understanding the Concept of Null Hypothesis in Chi Square

The null hypothesis in chi-square tests is essentially a statement of no effect or no relationship. When it comes to categorical data, it indicates that the distribution of categories for one variable is not affected by the distribution of categories of the other variable.

For example, if we compare the preference for different types of fruit among men and women, the null hypothesis would state that the preference is independent of gender. The alternative hypothesis, on the other hand, would suggest a dependency between the two.

Steps to Formulate the Null Hypothesis in Chi-Square Tests

Formulating the null hypothesis is a critical step in any chi-square test. First, identify the variables being tested. Then, once the variables are determined, the null hypothesis can be formulated to state no association between them.

Next, collect your data. This data must be frequencies or counts of categories, not percentages or averages. Once the data is collected, you can calculate the expected frequency for each category under the null hypothesis.

Finally, use the chi-square formula to calculate the chi-square statistic. This will help determine whether to reject or fail to reject the null hypothesis.

Practical Example and Case Study

Consider a study evaluating whether smoking status is independent of a lung cancer diagnosis. The null hypothesis would state that smoking status (smoker or non-smoker) is independent of cancer diagnosis (yes or no).

If we find a p-value less than our significance level (typically 0.05) after conducting the chi-square test, we would reject the null hypothesis and conclude that smoking status is not independent of lung cancer diagnosis, suggesting a significant association between the two.

Observed Table

Expected table, common misunderstandings and pitfalls.

One common misunderstanding is the interpretation of failing to reject the null hypothesis. It’s important to remember that failing to reject the null does not prove it true. Instead, it merely suggests that our data do not provide strong enough evidence against it.

Another pitfall is applying the chi-square test to inappropriate data. The chi-square test requires categorical or nominal data. Applying it to ordinal or continuous data without proper binning or categorization can lead to incorrect results.

The null hypothesis in chi-square testing is a powerful tool in statistical analysis. It provides a means to differentiate between observed variations due to random chance versus those that may signify a significant effect or relationship. As we continue to generate more data in various fields, the importance of understanding and correctly applying chi-square tests and the concept of the null hypothesis grows.

Recommended Articles

Interested in diving deeper into statistics? Explore our range of statistical analysis and data science articles to broaden your understanding. Visit our blog now!

• Simple Null Hypothesis – an overview (External Link)
• Chi-Square Calculator: Enhance Your Data Analysis Skills
• Effect Size for Chi-Square Tests: Unveiling its Significance
• What is the Difference Between the T-Test vs. Chi-Square Test?
• Understanding the Assumptions for Chi-Square Test of Independence
• How to Report Chi-Square Test Results in APA Style: A Step-By-Step Guide

Frequently Asked Questions (FAQs)

It’s a statistical test used to determine if there’s a significant association between two categorical variables.

The null hypothesis suggests no significant difference between observed and expected frequencies exists. The alternative hypothesis suggests a significant difference.

No, we never “accept” the null hypothesis. We only fail to reject it if the data doesn’t provide strong evidence against it.

Rejecting the null hypothesis implies a significant difference between observed and expected frequencies, suggesting an association between variables.

Chi-Square tests are appropriate for categorical or nominal data.

The significance level, often 0.05, is the probability threshold below which the null hypothesis can be rejected.

A p-value < the significance level indicates a significant association between variables, leading to rejecting the null hypothesis.

Using the Chi-Square test for improper data, like ordinal or continuous data, without proper categorization can lead to incorrect results.

Identify the variables, state their independence, collect data, calculate expected frequencies, and apply the Chi-Square formula.

Understanding the null hypothesis is essential for correctly interpreting and applying Chi-Square tests, helping to make informed decisions based on data.

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9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 66
• H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 45
• H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : p __ 0.40
• H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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9.4: Probability and Chi-Square Analysis

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Mendel’s Observations

Probability: Past Punnett Squares

Punnett Squares are convenient for predicting the outcome of monohybrid or dihybrid crosses. The expectation of two heterozygous parents is 3:1 in a single trait cross or 9:3:3:1 in a two-trait cross. Performing a three or four trait cross becomes very messy. In these instances, it is better to follow the rules of probability. Probability is the chance that an event will occur expressed as a fraction or percentage. In the case of a monohybrid cross, 3:1 ratio means that there is a \(\frac{3}{4}\) (0.75) chance of the dominant phenotype with a \(\frac{1}{4}\) (0.25) chance of a recessive phenotype.

A single die has a 1 in 6 chance of being a specific value. In this case, there is a \(\frac{1}{6}\) probability of rolling a 3. It is understood that rolling a second die simultaneously is not influenced by the first and is therefore independent. This second die also has a \(\frac{1}{6}\) chance of being a 3.

We can understand these rules of probability by applying them to the dihybrid cross and realizing we come to the same outcome as the 2 monohybrid Punnett Squares as with the single dihybrid Punnett Square.

This forked line method of calculating probability of offspring with various genotypes and phenotypes can be scaled and applied to more characteristics.

The Chi-Square Test

The χ 2 statistic is used in genetics to illustrate if there are deviations from the expected outcomes of the alleles in a population. The general assumption of any statistical test is that there are no significant deviations between the measured results and the predicted ones. This lack of deviation is called the null hypothesis ( H 0 ). X 2 statistic uses a distribution table to compare results against at varying levels of probabilities or critical values . If the X 2 value is greater than the value at a specific probability, then the null hypothesis has been rejected and a significant deviation from predicted values was observed. Using Mendel’s laws, we can count phenotypes after a cross to compare against those predicted by probabilities (or a Punnett Square).

In order to use the table, one must determine the stringency of the test. The lower the p-value, the more stringent the statistics. Degrees of Freedom ( DF ) are also calculated to determine which value on the table to use. Degrees of Freedom is the number of classes or categories there are in the observations minus 1. DF=n-1

In the example of corn kernel color and texture, there are 4 classes: Purple & Smooth, Purple & Wrinkled, Yellow & Smooth, Yellow & Wrinkled. Therefore, DF = 4 – 1 = 3 and choosing p < 0.05 to be the threshold for significance (rejection of the null hypothesis), the X 2 must be greater than 7.82 in order to be significantly deviating from what is expected. With this dihybrid cross example, we expect a ratio of 9:3:3:1 in phenotypes where 1/16th of the population are recessive for both texture and color while \(\frac{9}{16}\) of the population display both color and texture as the dominant. \(\frac{3}{16}\) will be dominant for one phenotype while recessive for the other and the remaining \(\frac{3}{16}\) will be the opposite combination.

With this in mind, we can predict or have expected outcomes using these ratios. Taking a total count of 200 events in a population, 9/16(200)=112.5 and so forth. Formally, the χ 2 value is generated by summing all combinations of:

\[\frac{(Observed-Expected)^2}{Expected}\]

Chi-Square Test: Is This Coin Fair or Weighted? (Activity)

• Everyone in the class should flip a coin 2x and record the result (assumes class is 24).
• 50% of 48 results should be 24.
• 24 heads and 24 tails are already written in the “Expected” column.
• As a class, compile the results in the “Observed” column (total of 48 coin flips).
• In the last column, subtract the expected heads from the observed heads and square it, then divide by the number of expected heads.
• In the last column, subtract the expected tails from the observed tails and square it, then divide by the number of expected tails.
• Add the values together from the last column to generate the X 2 value.
• There are 2 classes or categories (head or tail), so DF = 2 – 1 = 1.
• Were the coin flips fair (not significantly deviating from 50:50)?

Let’s say that the coin tosses yielded 26 Heads and 22 Tails. Can we assume that the coin was unfair? If we toss a coin an odd number of times (eg. 51), then we would expect that the results would yield 25.5 (50%) Heads and 25.5 (50%) Tails. But this isn’t a possibility. This is when the X 2 test is important as it delineates whether 26:25 or 30:21 etc. are within the probability for a fair coin.

Chi-Square Test of Kernel Coloration and Texture in an F 2 Population (Activity)

• From the counts, one can assume which phenotypes are dominant and recessive.
• Fill in the “Observed” category with the appropriate counts.
• Fill in the “Expected Ratio” with either 9/16, 3/16 or 1/16.
• The total number of the counted event was 200, so multiply the “Expected Ratio” x 200 to generate the “Expected Number” fields.
• Calculate the \(\frac{(Observed-Expected)^2}{Expected}\) for each phenotype combination
• Add all \(\frac{(Observed-Expected)^2}{Expected}\) values together to generate the X 2 value and compare with the value on the table where DF=3.
• What would it mean if the Null Hypothesis was rejected? Can you explain a case in which we have observed values that are significantly altered from what is expected?