abstract quantitative research example

• How to Write An Abstract For Research Papers: Tips & Examples

In many ways, an abstract is like a trailer of a movie or the synopsis of your favorite book. Its job is to whet the reader’s appetite by sharing important information about your work. After reading a well-written abstract, one should have enough interest to explore the full research thesis. 

So how do you write an interesting abstract that captures the core of your study? First, you need to understand your research objectives and match them with the key results of your study. In this article, we will share some tips for writing an effective abstract, plus samples you can learn from. 

What is an Abstract in Research Writing?

In simple terms, an abstract is a concise write-up that gives an overview of your systematic investigation. According to Grammarly, it is a self-contained summary of a larger work, and it serves as a preview of the bigger document. 

It usually appears at the beginning of your thesis or research paper and helps the reader to have an overview of your work without going into great detail. This means that when someone reads your abstract, it should give them a clear idea of the purpose of your systematic investigation, your problem statement, key results, and any gaps requiring further investigation. 

So how long should your abstract be to capture all of these details? The reality is you don’t need a lot of words to capture key pieces of information in your abstract. Typically, 6–7 sentences made up of 150–250 words should be just right. 

Read: Writing Research Proposals: Tips, Examples & Mistakes

What are the Characteristics of a Good Abstract? 

• A good abstract clearly states the aims and objectives of the research.
• It outlines the research methodology for data gathering , processing and analysis. 
• A good abstract summarizes specific research results.
• It states the key conclusions of the systematic investigation.
• It is brief yet straight to the point. 
• A good abstract is unified and coherent. 
• It is easy to understand and devoid of technical jargon. 
• It is written in an unbiased and objective manner. 

What is the Purpose of an Abstract? 

Every abstract has two major purposes. First, it communicates the relevance of your systematic investigation to readers. After reading your abstract, people can determine how relevant your study is to their primary or secondary research purpose. 

The second purpose of an abstract is to communicate your key findings to those who don’t have time to read the whole paper. Research papers typically run into tens of pages so it takes time to read and digest them. To help readers grasp the core ideas in a systematic investigation, it pays to have a well-written abstract that outlines important information concerning your study. 

In all, your abstract should accurately outline the most important information in your research. Many times, it determines whether people would go ahead to read your dissertation. Abstracts are often indexed along with keywords on academic databases, so they make your thesis easily findable.

Learn About: How to Write a Problem Statement for your Research

What are the Sections of an Abstract?

You already know the key pieces of information that your abstract should communicate. These details are broken into six important sections of the abstract which are: 

• The Introduction or Background
• Research Methodology
• Aims and Objectives 
• Limitations

Let’s discuss them in detail. 

• The Introduction or Background 

The introduction or background is the shortest part of your abstract and usually consists of 2–3 sentences. In fact, some researchers write a single sentence as the introduction of their abstract. The whole idea here is to take the reader through the important events leading to your research. 

Understandably, this information may appear difficult to convey in a few sentences. To help out, consider answering these two questions in the background to your study : 

• What is already known about the subject, related to the paper in question? 
• What is not known about the subject (this is the focus of your study)? 

As much as possible, ensure that your abstract’s introduction doesn’t eat into the word count for the other key information. 

• Research Methodology 

This is the section where you spell out any theories and methods adopted for your study. Ideally, you should cover what has been done and how you went about it to achieve the results of your systematic investigation. It is usually the second-longest section in the abstract. 

In the research methodology section, you should also state the type of research you embarked on; that is, qualitative research or quantitative research —this will inform your research methods too. If you’ve conducted quantitative research, your abstract should contain information like the sample size, data collection methods , sampling technique, and duration of your experiment. 

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In the end, readers are most interested in the results you’ve achieved with your study. This means you should take time to outline every relevant outcome and show how they affect your research population . Typically, the results section should be the longest one in your abstract and nothing should compromise its range and quality. 

An important thing you should do here is spelled out facts and figures about research outcomes. Instead of a vague statement like, “we noticed that response rates differed greatly between high-income and low-income respondents”, try this: “The response rate was higher in high-income respondents than in their low-income counterparts (59% vs 30%, respectively; P

• Conclusion 

Like the introduction, your conclusion should contain a few sentences that wrap up your abstract. Most researchers express a theoretical opinion about the implications of their study, here. 

Your conclusion should contain three important elements: 

• The primary take-home message
• The additional findings of importance
• The perspective 

Although the conclusion of your abstract should be short, it has a great impact on how readers perceive your study. So, take advantage of this section to reiterate the core message in your systematic investigation. Also, make sure any statements here reflect the true outcomes and methods of your research. 

• Limitations 

Chances are you must have faced certain challenges in the course of your research—it could be at the data collection phase or during sampling . Whatever these challenges are, it pays to let your readers know about them, and the impact they had on your study. 

For example, if you had to switch to convenience sampling or snowball sampling due to difficulties in contacting well-suited research participants, you should include this in your abstract. Also, a lack of previous studies in the research area could pose a limitation on your study. Research limitations provide an opportunity to make suggestions for further research. 

Research aims and objectives speak to what you want to achieve with your study. Typically, research aims focus on a project’s long-term outcomes while the objectives focus on the immediate, short-term outcome of the investigation. You may summarize both using a single paragraph comprising a few sentences.

Stating your aims and objectives will give readers a clear idea of the scope, depth, and direction that your research will ultimately take. Readers would measure your research outcomes against stated aims and objectives to know if you achieved the purpose of your study. 

Use For Free: Research Form Templates

Abstract Writing Styles and General Guidelines 

Now that you know the different sections plus information that your abstract should contain, let’s look at how to write an abstract for your research paper.

A common question that comes up is, should I write my abstract first or last? It’s best to write your abstract after you’ve finished working on the research because you have full information to present to your readers. However, you can always create a draft at the beginning of your systematic investigation and fill in the gaps later.  

Does writing an abstract seem like a herculean task? Here are a few tips to help out. 

1. Always create a framework for your abstract 

Before you start writing, take time to develop a detailed outline for your abstract. Break it into sections and sketch the main and supporting points for each section. You can list keywords plus 1–2 sentences that capture your core messaging. 

2. Read Other Abstracts 

Abstracts are one of the most common research documents, and thousands of them have been written in time past. So, before writing yours, try to study a couple of samples from others. You can find lots of dissertation abstract examples in thesis and dissertation databases.

3. Steer Clear of Jargon As Much As Possible 

While writing your abstract, emphasize clarity over style. This means you should communicate in simple terms and avoid unnecessary filler words and ambiguous sentences. Remember, your abstract should be understandable to readers who are not familiar with your topic. 

4. Focus on Your Research

It goes without saying that your abstract should be solely focused on your research and what you’ve discovered. It’s not the time to cite primary and secondary data sources unless this is absolutely necessary. 

This doesn’t mean you should ignore the scholarly background of your work. You might include a sentence or two summarizing the scholarly background to show the relevance of your work to a broader debate, but there’s no need to mention specific publications. 

Going further, here are some abstract writing guidelines from the University of Bergen: 

• An abstract briefly explains the salient aspects of the content. 
• Abstracts should be accurate and succinct, self-contained, and readable.  
• The abstract should paraphrase and summarise rather than quote from the paper.
• Abstracts should relate only to the paper to be presented/assessed.

Types of Abstracts with Examples 

According to the University of Adelaide, there are two major types of abstracts written for research purposes. First, we have informative abstracts and descriptive abstracts. 

1. Informative Abstract  

An informative abstract is the more common type of abstract written for academic research. It highlights the most important aspects of your systematic investigation without going into unnecessary or irrelevant details that the reader might not find useful. 

The length varies according to discipline, but an informative abstract is rarely more than 10% of the length of the entire work. In the case of longer work, it may be much less.

In any informative abstract, you’d touch on information like the purpose, method, scope, results, and conclusion of your study. By now, you’re thinking, “this is the type of abstract we’ve been discussing all along”, and you wouldn’t be far from the truth. 

Advantages of Informative Abstracts

• These abstracts save time for both the researcher and the readers. 
• It’s easy to refer to these abstracts as secondary research sources. 

Disadvantages of Informative Abstracts

• These types of abstracts lack personality.

Example of an Informative Abstract

• Sample Informative Abstract Based on Experimental Work From Colorado State University
• Sample Informative Abstract Based on Non-experimental Work From Colorado State University

2. Descriptive Abstract 

A descriptive abstract reads like a synopsis and focuses on enticing the reader with interesting information. They don’t care as much for data and details, and instead read more like overviews that don’t give too much away. 

You’d find descriptive abstracts in artistic criticism pieces and entertainment research as opposed to scientific investigations. This type of abstract makes no judgments about the work, nor does it provide results or conclusions of the research. They are usually written in 100 words or less. 

Advantages of Descriptive Abstracts

• It gives a very brief overview of the research paper. 
• It is easier to write descriptive abstracts compared to informational abstracts. 

Disadvantages of Descriptive Abstracts

• They are suitable for scientific research. 
• Descriptive abstracts might omit relevant information that deepens your knowledge of the systematic investigation.

Example of Descriptive Abstracts 

• Sample Descriptive Abstract From Colorado State University

FAQs About Writing Abstracts in Research Papers

1. How Long Should an Abstract Be?

A typical abstract should be about six sentences long or less than 150 words. Most universities have specific word count requirements that fall within 150–300 words. 

2. How Do You Start an Abstract Sentence?

There are several ways to start your abstract. Consider the following methods: 

• State a problem or uncertainty
• Make a general statement with the present research action.
• State the purpose or objective of your research
• State a real-world phenomena or a standard practice.

3. Should you cite in an abstract?

While you can refer to information from specific research papers, there’s no need to cite sources in your abstract. Your abstract should focus on your original research, not on the work of others. 

4. What should not be included in an abstract?

An abstract shouldn’t have numeric references, bibliographies, sections, or even footnotes. 

5. Which tense is used in writing an abstract?

An abstract should be written in the third-person present tense. Use the simple past tense when describing your methodology and specific findings from your study. 

Writing an abstract might appear challenging but with these steps, you should get it right. The easiest approach to writing a good abstract is centering it on key information including your research problem and objectives, methodology, and key results.

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Writing an Abstract for Your Research Paper

Definition and Purpose of Abstracts

An abstract is a short summary of your (published or unpublished) research paper, usually about a paragraph (c. 6-7 sentences, 150-250 words) long. A well-written abstract serves multiple purposes:

• an abstract lets readers get the gist or essence of your paper or article quickly, in order to decide whether to read the full paper;
• an abstract prepares readers to follow the detailed information, analyses, and arguments in your full paper;
• and, later, an abstract helps readers remember key points from your paper.

It’s also worth remembering that search engines and bibliographic databases use abstracts, as well as the title, to identify key terms for indexing your published paper. So what you include in your abstract and in your title are crucial for helping other researchers find your paper or article.

If you are writing an abstract for a course paper, your professor may give you specific guidelines for what to include and how to organize your abstract. Similarly, academic journals often have specific requirements for abstracts. So in addition to following the advice on this page, you should be sure to look for and follow any guidelines from the course or journal you’re writing for.

The Contents of an Abstract

Abstracts contain most of the following kinds of information in brief form. The body of your paper will, of course, develop and explain these ideas much more fully. As you will see in the samples below, the proportion of your abstract that you devote to each kind of information—and the sequence of that information—will vary, depending on the nature and genre of the paper that you are summarizing in your abstract. And in some cases, some of this information is implied, rather than stated explicitly. The Publication Manual of the American Psychological Association , which is widely used in the social sciences, gives specific guidelines for what to include in the abstract for different kinds of papers—for empirical studies, literature reviews or meta-analyses, theoretical papers, methodological papers, and case studies.

Here are the typical kinds of information found in most abstracts:

• the context or background information for your research; the general topic under study; the specific topic of your research
• the central questions or statement of the problem your research addresses
• what’s already known about this question, what previous research has done or shown
• the main reason(s) , the exigency, the rationale , the goals for your research—Why is it important to address these questions? Are you, for example, examining a new topic? Why is that topic worth examining? Are you filling a gap in previous research? Applying new methods to take a fresh look at existing ideas or data? Resolving a dispute within the literature in your field? . . .
• your research and/or analytical methods
• your main findings , results , or arguments
• the significance or implications of your findings or arguments.

Your abstract should be intelligible on its own, without a reader’s having to read your entire paper. And in an abstract, you usually do not cite references—most of your abstract will describe what you have studied in your research and what you have found and what you argue in your paper. In the body of your paper, you will cite the specific literature that informs your research.

When to Write Your Abstract

Although you might be tempted to write your abstract first because it will appear as the very first part of your paper, it’s a good idea to wait to write your abstract until after you’ve drafted your full paper, so that you know what you’re summarizing.

What follows are some sample abstracts in published papers or articles, all written by faculty at UW-Madison who come from a variety of disciplines. We have annotated these samples to help you see the work that these authors are doing within their abstracts.

Choosing Verb Tenses within Your Abstract

The social science sample (Sample 1) below uses the present tense to describe general facts and interpretations that have been and are currently true, including the prevailing explanation for the social phenomenon under study. That abstract also uses the present tense to describe the methods, the findings, the arguments, and the implications of the findings from their new research study. The authors use the past tense to describe previous research.

The humanities sample (Sample 2) below uses the past tense to describe completed events in the past (the texts created in the pulp fiction industry in the 1970s and 80s) and uses the present tense to describe what is happening in those texts, to explain the significance or meaning of those texts, and to describe the arguments presented in the article.

The science samples (Samples 3 and 4) below use the past tense to describe what previous research studies have done and the research the authors have conducted, the methods they have followed, and what they have found. In their rationale or justification for their research (what remains to be done), they use the present tense. They also use the present tense to introduce their study (in Sample 3, “Here we report . . .”) and to explain the significance of their study (In Sample 3, This reprogramming . . . “provides a scalable cell source for. . .”).

Sample Abstract 1

From the social sciences.

Reporting new findings about the reasons for increasing economic homogamy among spouses

Gonalons-Pons, Pilar, and Christine R. Schwartz. “Trends in Economic Homogamy: Changes in Assortative Mating or the Division of Labor in Marriage?” Demography , vol. 54, no. 3, 2017, pp. 985-1005.

Sample Abstract 2

From the humanities.

Analyzing underground pulp fiction publications in Tanzania, this article makes an argument about the cultural significance of those publications

Emily Callaci. “Street Textuality: Socialism, Masculinity, and Urban Belonging in Tanzania’s Pulp Fiction Publishing Industry, 1975-1985.” Comparative Studies in Society and History , vol. 59, no. 1, 2017, pp. 183-210.

Sample Abstract/Summary 3

From the sciences.

Reporting a new method for reprogramming adult mouse fibroblasts into induced cardiac progenitor cells

Lalit, Pratik A., Max R. Salick, Daryl O. Nelson, Jayne M. Squirrell, Christina M. Shafer, Neel G. Patel, Imaan Saeed, Eric G. Schmuck, Yogananda S. Markandeya, Rachel Wong, Martin R. Lea, Kevin W. Eliceiri, Timothy A. Hacker, Wendy C. Crone, Michael Kyba, Daniel J. Garry, Ron Stewart, James A. Thomson, Karen M. Downs, Gary E. Lyons, and Timothy J. Kamp. “Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors.” Cell Stem Cell , vol. 18, 2016, pp. 354-367.

Sample Abstract 4, a Structured Abstract

Reporting results about the effectiveness of antibiotic therapy in managing acute bacterial sinusitis, from a rigorously controlled study

Note: This journal requires authors to organize their abstract into four specific sections, with strict word limits. Because the headings for this structured abstract are self-explanatory, we have chosen not to add annotations to this sample abstract.

Wald, Ellen R., David Nash, and Jens Eickhoff. “Effectiveness of Amoxicillin/Clavulanate Potassium in the Treatment of Acute Bacterial Sinusitis in Children.” Pediatrics , vol. 124, no. 1, 2009, pp. 9-15.

“OBJECTIVE: The role of antibiotic therapy in managing acute bacterial sinusitis (ABS) in children is controversial. The purpose of this study was to determine the effectiveness of high-dose amoxicillin/potassium clavulanate in the treatment of children diagnosed with ABS.

METHODS : This was a randomized, double-blind, placebo-controlled study. Children 1 to 10 years of age with a clinical presentation compatible with ABS were eligible for participation. Patients were stratified according to age (<6 or ≥6 years) and clinical severity and randomly assigned to receive either amoxicillin (90 mg/kg) with potassium clavulanate (6.4 mg/kg) or placebo. A symptom survey was performed on days 0, 1, 2, 3, 5, 7, 10, 20, and 30. Patients were examined on day 14. Children’s conditions were rated as cured, improved, or failed according to scoring rules.

RESULTS: Two thousand one hundred thirty-five children with respiratory complaints were screened for enrollment; 139 (6.5%) had ABS. Fifty-eight patients were enrolled, and 56 were randomly assigned. The mean age was 6630 months. Fifty (89%) patients presented with persistent symptoms, and 6 (11%) presented with nonpersistent symptoms. In 24 (43%) children, the illness was classified as mild, whereas in the remaining 32 (57%) children it was severe. Of the 28 children who received the antibiotic, 14 (50%) were cured, 4 (14%) were improved, 4(14%) experienced treatment failure, and 6 (21%) withdrew. Of the 28children who received placebo, 4 (14%) were cured, 5 (18%) improved, and 19 (68%) experienced treatment failure. Children receiving the antibiotic were more likely to be cured (50% vs 14%) and less likely to have treatment failure (14% vs 68%) than children receiving the placebo.

CONCLUSIONS : ABS is a common complication of viral upper respiratory infections. Amoxicillin/potassium clavulanate results in significantly more cures and fewer failures than placebo, according to parental report of time to resolution.” (9)

Some Excellent Advice about Writing Abstracts for Basic Science Research Papers, by Professor Adriano Aguzzi from the Institute of Neuropathology at the University of Zurich:

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An abstract summarizes, usually in one paragraph of 300 words or less, the major aspects of the entire paper in a prescribed sequence that includes: 1) the overall purpose of the study and the research problem(s) you investigated; 2) the basic design of the study; 3) major findings or trends found as a result of your analysis; and, 4) a brief summary of your interpretations and conclusions.

Writing an Abstract. The Writing Center. Clarion University, 2009; Writing an Abstract for Your Research Paper. The Writing Center, University of Wisconsin, Madison; Koltay, Tibor. Abstracts and Abstracting: A Genre and Set of Skills for the Twenty-first Century . Oxford, UK: Chandos Publishing, 2010;

Importance of a Good Abstract

Sometimes your professor will ask you to include an abstract, or general summary of your work, with your research paper. The abstract allows you to elaborate upon each major aspect of the paper and helps readers decide whether they want to read the rest of the paper. Therefore, enough key information [e.g., summary results, observations, trends, etc.] must be included to make the abstract useful to someone who may want to examine your work.

How do you know when you have enough information in your abstract? A simple rule-of-thumb is to imagine that you are another researcher doing a similar study. Then ask yourself: if your abstract was the only part of the paper you could access, would you be happy with the amount of information presented there? Does it tell the whole story about your study? If the answer is "no" then the abstract likely needs to be revised.

Farkas, David K. “A Scheme for Understanding and Writing Summaries.” Technical Communication 67 (August 2020): 45-60;  How to Write a Research Abstract. Office of Undergraduate Research. University of Kentucky; Staiger, David L. “What Today’s Students Need to Know about Writing Abstracts.” International Journal of Business Communication January 3 (1966): 29-33; Swales, John M. and Christine B. Feak. Abstracts and the Writing of Abstracts . Ann Arbor, MI: University of Michigan Press, 2009.

Structure and Writing Style

I.  Types of Abstracts

To begin, you need to determine which type of abstract you should include with your paper. There are four general types.

Critical Abstract A critical abstract provides, in addition to describing main findings and information, a judgment or comment about the study’s validity, reliability, or completeness. The researcher evaluates the paper and often compares it with other works on the same subject. Critical abstracts are generally 400-500 words in length due to the additional interpretive commentary. These types of abstracts are used infrequently.

Descriptive Abstract A descriptive abstract indicates the type of information found in the work. It makes no judgments about the work, nor does it provide results or conclusions of the research. It does incorporate key words found in the text and may include the purpose, methods, and scope of the research. Essentially, the descriptive abstract only describes the work being summarized. Some researchers consider it an outline of the work, rather than a summary. Descriptive abstracts are usually very short, 100 words or less. Informative Abstract The majority of abstracts are informative. While they still do not critique or evaluate a work, they do more than describe it. A good informative abstract acts as a surrogate for the work itself. That is, the researcher presents and explains all the main arguments and the important results and evidence in the paper. An informative abstract includes the information that can be found in a descriptive abstract [purpose, methods, scope] but it also includes the results and conclusions of the research and the recommendations of the author. The length varies according to discipline, but an informative abstract is usually no more than 300 words in length.

Highlight Abstract A highlight abstract is specifically written to attract the reader’s attention to the study. No pretense is made of there being either a balanced or complete picture of the paper and, in fact, incomplete and leading remarks may be used to spark the reader’s interest. In that a highlight abstract cannot stand independent of its associated article, it is not a true abstract and, therefore, rarely used in academic writing.

II.  Writing Style

Use the active voice when possible , but note that much of your abstract may require passive sentence constructions. Regardless, write your abstract using concise, but complete, sentences. Get to the point quickly and always use the past tense because you are reporting on a study that has been completed.

Abstracts should be formatted as a single paragraph in a block format and with no paragraph indentations. In most cases, the abstract page immediately follows the title page. Do not number the page. Rules set forth in writing manual vary but, in general, you should center the word "Abstract" at the top of the page with double spacing between the heading and the abstract. The final sentences of an abstract concisely summarize your study’s conclusions, implications, or applications to practice and, if appropriate, can be followed by a statement about the need for additional research revealed from the findings.

Composing Your Abstract

Although it is the first section of your paper, the abstract should be written last since it will summarize the contents of your entire paper. A good strategy to begin composing your abstract is to take whole sentences or key phrases from each section of the paper and put them in a sequence that summarizes the contents. Then revise or add connecting phrases or words to make the narrative flow clearly and smoothly. Note that statistical findings should be reported parenthetically [i.e., written in parentheses].

Before handing in your final paper, check to make sure that the information in the abstract completely agrees with what you have written in the paper. Think of the abstract as a sequential set of complete sentences describing the most crucial information using the fewest necessary words. The abstract SHOULD NOT contain:

• A catchy introductory phrase, provocative quote, or other device to grab the reader's attention,
• Lengthy background or contextual information,
• Redundant phrases, unnecessary adverbs and adjectives, and repetitive information;
• Acronyms or abbreviations,
• References to other literature [say something like, "current research shows that..." or "studies have indicated..."],
• Using ellipticals [i.e., ending with "..."] or incomplete sentences,
• Jargon or terms that may be confusing to the reader,
• Citations to other works, and
• Any sort of image, illustration, figure, or table, or references to them.

Abstract. Writing Center. University of Kansas; Abstract. The Structure, Format, Content, and Style of a Journal-Style Scientific Paper. Department of Biology. Bates College; Abstracts. The Writing Center. University of North Carolina; Borko, Harold and Seymour Chatman. "Criteria for Acceptable Abstracts: A Survey of Abstracters' Instructions." American Documentation 14 (April 1963): 149-160; Abstracts. The Writer’s Handbook. Writing Center. University of Wisconsin, Madison; Hartley, James and Lucy Betts. "Common Weaknesses in Traditional Abstracts in the Social Sciences." Journal of the American Society for Information Science and Technology 60 (October 2009): 2010-2018; Koltay, Tibor. Abstracts and Abstracting: A Genre and Set of Skills for the Twenty-first Century. Oxford, UK: Chandos Publishing, 2010; Procter, Margaret. The Abstract. University College Writing Centre. University of Toronto; Riordan, Laura. “Mastering the Art of Abstracts.” The Journal of the American Osteopathic Association 115 (January 2015 ): 41-47; Writing Report Abstracts. The Writing Lab and The OWL. Purdue University; Writing Abstracts. Writing Tutorial Services, Center for Innovative Teaching and Learning. Indiana University; Koltay, Tibor. Abstracts and Abstracting: A Genre and Set of Skills for the Twenty-First Century . Oxford, UK: 2010; Writing an Abstract for Your Research Paper. The Writing Center, University of Wisconsin, Madison.

Writing Tip

Never Cite Just the Abstract!

Citing to just a journal article's abstract does not confirm for the reader that you have conducted a thorough or reliable review of the literature. If the full-text is not available, go to the USC Libraries main page and enter the title of the article [NOT the title of the journal]. If the Libraries have a subscription to the journal, the article should appear with a link to the full-text or to the journal publisher page where you can get the article. If the article does not appear, try searching Google Scholar using the link on the USC Libraries main page. If you still can't find the article after doing this, contact a librarian or you can request it from our free i nterlibrary loan and document delivery service .

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• How to Write an Abstract | Steps & Examples

How to Write an Abstract | Steps & Examples

Published on 1 March 2019 by Shona McCombes . Revised on 10 October 2022 by Eoghan Ryan.

An abstract is a short summary of a longer work (such as a dissertation or research paper ). The abstract concisely reports the aims and outcomes of your research, so that readers know exactly what your paper is about.

Although the structure may vary slightly depending on your discipline, your abstract should describe the purpose of your work, the methods you’ve used, and the conclusions you’ve drawn.

One common way to structure your abstract is to use the IMRaD structure. This stands for:

• Introduction

Abstracts are usually around 100–300 words, but there’s often a strict word limit, so make sure to check the relevant requirements.

In a dissertation or thesis , include the abstract on a separate page, after the title page and acknowledgements but before the table of contents .

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Table of contents

Abstract example, when to write an abstract, step 1: introduction, step 2: methods, step 3: results, step 4: discussion, tips for writing an abstract, frequently asked questions about abstracts.

Hover over the different parts of the abstract to see how it is constructed.

This paper examines the role of silent movies as a mode of shared experience in the UK during the early twentieth century. At this time, high immigration rates resulted in a significant percentage of non-English-speaking citizens. These immigrants faced numerous economic and social obstacles, including exclusion from public entertainment and modes of discourse (newspapers, theater, radio).

Incorporating evidence from reviews, personal correspondence, and diaries, this study demonstrates that silent films were an affordable and inclusive source of entertainment. It argues for the accessible economic and representational nature of early cinema. These concerns are particularly evident in the low price of admission and in the democratic nature of the actors’ exaggerated gestures, which allowed the plots and action to be easily grasped by a diverse audience despite language barriers.

Keywords: silent movies, immigration, public discourse, entertainment, early cinema, language barriers.

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You will almost always have to include an abstract when:

• Completing a thesis or dissertation
• Submitting a research paper to an academic journal
• Writing a book proposal
• Applying for research grants

It’s easiest to write your abstract last, because it’s a summary of the work you’ve already done. Your abstract should:

• Be a self-contained text, not an excerpt from your paper
• Be fully understandable on its own
• Reflect the structure of your larger work

Start by clearly defining the purpose of your research. What practical or theoretical problem does the research respond to, or what research question did you aim to answer?

You can include some brief context on the social or academic relevance of your topic, but don’t go into detailed background information. If your abstract uses specialised terms that would be unfamiliar to the average academic reader or that have various different meanings, give a concise definition.

After identifying the problem, state the objective of your research. Use verbs like “investigate,” “test,” “analyse,” or “evaluate” to describe exactly what you set out to do.

This part of the abstract can be written in the present or past simple tense  but should never refer to the future, as the research is already complete.

• This study will investigate the relationship between coffee consumption and productivity.
• This study investigates the relationship between coffee consumption and productivity.

Next, indicate the research methods that you used to answer your question. This part should be a straightforward description of what you did in one or two sentences. It is usually written in the past simple tense, as it refers to completed actions.

• Structured interviews will be conducted with 25 participants.
• Structured interviews were conducted with 25 participants.

Don’t evaluate validity or obstacles here — the goal is not to give an account of the methodology’s strengths and weaknesses, but to give the reader a quick insight into the overall approach and procedures you used.

Next, summarise the main research results . This part of the abstract can be in the present or past simple tense.

• Our analysis has shown a strong correlation between coffee consumption and productivity.
• Our analysis shows a strong correlation between coffee consumption and productivity.
• Our analysis showed a strong correlation between coffee consumption and productivity.

Depending on how long and complex your research is, you may not be able to include all results here. Try to highlight only the most important findings that will allow the reader to understand your conclusions.

Finally, you should discuss the main conclusions of your research : what is your answer to the problem or question? The reader should finish with a clear understanding of the central point that your research has proved or argued. Conclusions are usually written in the present simple tense.

• We concluded that coffee consumption increases productivity.
• We conclude that coffee consumption increases productivity.

If there are important limitations to your research (for example, related to your sample size or methods), you should mention them briefly in the abstract. This allows the reader to accurately assess the credibility and generalisability of your research.

If your aim was to solve a practical problem, your discussion might include recommendations for implementation. If relevant, you can briefly make suggestions for further research.

If your paper will be published, you might have to add a list of keywords at the end of the abstract. These keywords should reference the most important elements of the research to help potential readers find your paper during their own literature searches.

Be aware that some publication manuals, such as APA Style , have specific formatting requirements for these keywords.

It can be a real challenge to condense your whole work into just a couple of hundred words, but the abstract will be the first (and sometimes only) part that people read, so it’s important to get it right. These strategies can help you get started.

Read other abstracts

The best way to learn the conventions of writing an abstract in your discipline is to read other people’s. You probably already read lots of journal article abstracts while conducting your literature review —try using them as a framework for structure and style.

You can also find lots of dissertation abstract examples in thesis and dissertation databases .

Reverse outline

Not all abstracts will contain precisely the same elements. For longer works, you can write your abstract through a process of reverse outlining.

For each chapter or section, list keywords and draft one to two sentences that summarise the central point or argument. This will give you a framework of your abstract’s structure. Next, revise the sentences to make connections and show how the argument develops.

Write clearly and concisely

A good abstract is short but impactful, so make sure every word counts. Each sentence should clearly communicate one main point.

To keep your abstract or summary short and clear:

• Avoid passive sentences: Passive constructions are often unnecessarily long. You can easily make them shorter and clearer by using the active voice.
• Avoid long sentences: Substitute longer expressions for concise expressions or single words (e.g., “In order to” for “To”).
• Avoid obscure jargon: The abstract should be understandable to readers who are not familiar with your topic.
• Avoid repetition and filler words: Replace nouns with pronouns when possible and eliminate unnecessary words.
• Avoid detailed descriptions: An abstract is not expected to provide detailed definitions, background information, or discussions of other scholars’ work. Instead, include this information in the body of your thesis or paper.

If you’re struggling to edit down to the required length, you can get help from expert editors with Scribbr’s professional proofreading services .

Check your formatting

If you are writing a thesis or dissertation or submitting to a journal, there are often specific formatting requirements for the abstract—make sure to check the guidelines and format your work correctly. For APA research papers you can follow the APA abstract format .

Checklist: Abstract

The word count is within the required length, or a maximum of one page.

The abstract appears after the title page and acknowledgements and before the table of contents .

I have clearly stated my research problem and objectives.

I have briefly described my methodology .

I have summarized the most important results .

I have stated my main conclusions .

I have mentioned any important limitations and recommendations.

The abstract can be understood by someone without prior knowledge of the topic.

You've written a great abstract! Use the other checklists to continue improving your thesis or dissertation.

An abstract is a concise summary of an academic text (such as a journal article or dissertation ). It serves two main purposes:

• To help potential readers determine the relevance of your paper for their own research.
• To communicate your key findings to those who don’t have time to read the whole paper.

Abstracts are often indexed along with keywords on academic databases, so they make your work more easily findable. Since the abstract is the first thing any reader sees, it’s important that it clearly and accurately summarises the contents of your paper.

An abstract for a thesis or dissertation is usually around 150–300 words. There’s often a strict word limit, so make sure to check your university’s requirements.

The abstract is the very last thing you write. You should only write it after your research is complete, so that you can accurately summarize the entirety of your thesis or paper.

Avoid citing sources in your abstract . There are two reasons for this:

• The abstract should focus on your original research, not on the work of others.
• The abstract should be self-contained and fully understandable without reference to other sources.

There are some circumstances where you might need to mention other sources in an abstract: for example, if your research responds directly to another study or focuses on the work of a single theorist. In general, though, don’t include citations unless absolutely necessary.

The abstract appears on its own page, after the title page and acknowledgements but before the table of contents .

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What this handout is about

This handout provides definitions and examples of the two main types of abstracts: descriptive and informative. It also provides guidelines for constructing an abstract and general tips for you to keep in mind when drafting. Finally, it includes a few examples of abstracts broken down into their component parts.

What is an abstract?

An abstract is a self-contained, short, and powerful statement that describes a larger work. Components vary according to discipline. An abstract of a social science or scientific work may contain the scope, purpose, results, and contents of the work. An abstract of a humanities work may contain the thesis, background, and conclusion of the larger work. An abstract is not a review, nor does it evaluate the work being abstracted. While it contains key words found in the larger work, the abstract is an original document rather than an excerpted passage.

Why write an abstract?

You may write an abstract for various reasons. The two most important are selection and indexing. Abstracts allow readers who may be interested in a longer work to quickly decide whether it is worth their time to read it. Also, many online databases use abstracts to index larger works. Therefore, abstracts should contain keywords and phrases that allow for easy searching.

Say you are beginning a research project on how Brazilian newspapers helped Brazil’s ultra-liberal president Luiz Ignácio da Silva wrest power from the traditional, conservative power base. A good first place to start your research is to search Dissertation Abstracts International for all dissertations that deal with the interaction between newspapers and politics. “Newspapers and politics” returned 569 hits. A more selective search of “newspapers and Brazil” returned 22 hits. That is still a fair number of dissertations. Titles can sometimes help winnow the field, but many titles are not very descriptive. For example, one dissertation is titled “Rhetoric and Riot in Rio de Janeiro.” It is unclear from the title what this dissertation has to do with newspapers in Brazil. One option would be to download or order the entire dissertation on the chance that it might speak specifically to the topic. A better option is to read the abstract. In this case, the abstract reveals the main focus of the dissertation:

This dissertation examines the role of newspaper editors in the political turmoil and strife that characterized late First Empire Rio de Janeiro (1827-1831). Newspaper editors and their journals helped change the political culture of late First Empire Rio de Janeiro by involving the people in the discussion of state. This change in political culture is apparent in Emperor Pedro I’s gradual loss of control over the mechanisms of power. As the newspapers became more numerous and powerful, the Emperor lost his legitimacy in the eyes of the people. To explore the role of the newspapers in the political events of the late First Empire, this dissertation analyzes all available newspapers published in Rio de Janeiro from 1827 to 1831. Newspapers and their editors were leading forces in the effort to remove power from the hands of the ruling elite and place it under the control of the people. In the process, newspapers helped change how politics operated in the constitutional monarchy of Brazil.

From this abstract you now know that although the dissertation has nothing to do with modern Brazilian politics, it does cover the role of newspapers in changing traditional mechanisms of power. After reading the abstract, you can make an informed judgment about whether the dissertation would be worthwhile to read.

Besides selection, the other main purpose of the abstract is for indexing. Most article databases in the online catalog of the library enable you to search abstracts. This allows for quick retrieval by users and limits the extraneous items recalled by a “full-text” search. However, for an abstract to be useful in an online retrieval system, it must incorporate the key terms that a potential researcher would use to search. For example, if you search Dissertation Abstracts International using the keywords “France” “revolution” and “politics,” the search engine would search through all the abstracts in the database that included those three words. Without an abstract, the search engine would be forced to search titles, which, as we have seen, may not be fruitful, or else search the full text. It’s likely that a lot more than 60 dissertations have been written with those three words somewhere in the body of the entire work. By incorporating keywords into the abstract, the author emphasizes the central topics of the work and gives prospective readers enough information to make an informed judgment about the applicability of the work.

When do people write abstracts?

• when submitting articles to journals, especially online journals
• when applying for research grants
• when writing a book proposal
• when completing the Ph.D. dissertation or M.A. thesis
• when writing a proposal for a conference paper
• when writing a proposal for a book chapter

Most often, the author of the entire work (or prospective work) writes the abstract. However, there are professional abstracting services that hire writers to draft abstracts of other people’s work. In a work with multiple authors, the first author usually writes the abstract. Undergraduates are sometimes asked to draft abstracts of books/articles for classmates who have not read the larger work.

Types of abstracts

There are two types of abstracts: descriptive and informative. They have different aims, so as a consequence they have different components and styles. There is also a third type called critical, but it is rarely used. If you want to find out more about writing a critique or a review of a work, see the UNC Writing Center handout on writing a literature review . If you are unsure which type of abstract you should write, ask your instructor (if the abstract is for a class) or read other abstracts in your field or in the journal where you are submitting your article.

Descriptive abstracts

A descriptive abstract indicates the type of information found in the work. It makes no judgments about the work, nor does it provide results or conclusions of the research. It does incorporate key words found in the text and may include the purpose, methods, and scope of the research. Essentially, the descriptive abstract describes the work being abstracted. Some people consider it an outline of the work, rather than a summary. Descriptive abstracts are usually very short—100 words or less.

Informative abstracts

The majority of abstracts are informative. While they still do not critique or evaluate a work, they do more than describe it. A good informative abstract acts as a surrogate for the work itself. That is, the writer presents and explains all the main arguments and the important results and evidence in the complete article/paper/book. An informative abstract includes the information that can be found in a descriptive abstract (purpose, methods, scope) but also includes the results and conclusions of the research and the recommendations of the author. The length varies according to discipline, but an informative abstract is rarely more than 10% of the length of the entire work. In the case of a longer work, it may be much less.

Here are examples of a descriptive and an informative abstract of this handout on abstracts . Descriptive abstract:

The two most common abstract types—descriptive and informative—are described and examples of each are provided.

Informative abstract:

Abstracts present the essential elements of a longer work in a short and powerful statement. The purpose of an abstract is to provide prospective readers the opportunity to judge the relevance of the longer work to their projects. Abstracts also include the key terms found in the longer work and the purpose and methods of the research. Authors abstract various longer works, including book proposals, dissertations, and online journal articles. There are two main types of abstracts: descriptive and informative. A descriptive abstract briefly describes the longer work, while an informative abstract presents all the main arguments and important results. This handout provides examples of various types of abstracts and instructions on how to construct one.

Which type should I use?

Your best bet in this case is to ask your instructor or refer to the instructions provided by the publisher. You can also make a guess based on the length allowed; i.e., 100-120 words = descriptive; 250+ words = informative.

How do I write an abstract?

The format of your abstract will depend on the work being abstracted. An abstract of a scientific research paper will contain elements not found in an abstract of a literature article, and vice versa. However, all abstracts share several mandatory components, and there are also some optional parts that you can decide to include or not. When preparing to draft your abstract, keep the following key process elements in mind:

• Reason for writing: What is the importance of the research? Why would a reader be interested in the larger work?
• Problem: What problem does this work attempt to solve? What is the scope of the project? What is the main argument/thesis/claim?
• Methodology: An abstract of a scientific work may include specific models or approaches used in the larger study. Other abstracts may describe the types of evidence used in the research.
• Results: Again, an abstract of a scientific work may include specific data that indicates the results of the project. Other abstracts may discuss the findings in a more general way.
• Implications: What changes should be implemented as a result of the findings of the work? How does this work add to the body of knowledge on the topic?

(This list of elements is adapted with permission from Philip Koopman, “How to Write an Abstract.” )

All abstracts include:

• A full citation of the source, preceding the abstract.
• The most important information first.
• The same type and style of language found in the original, including technical language.
• Key words and phrases that quickly identify the content and focus of the work.
• Clear, concise, and powerful language.

Abstracts may include:

• The thesis of the work, usually in the first sentence.
• Background information that places the work in the larger body of literature.
• The same chronological structure as the original work.

How not to write an abstract:

• Do not refer extensively to other works.
• Do not add information not contained in the original work.
• Do not define terms.

If you are abstracting your own writing

When abstracting your own work, it may be difficult to condense a piece of writing that you have agonized over for weeks (or months, or even years) into a 250-word statement. There are some tricks that you could use to make it easier, however.

Reverse outlining:

This technique is commonly used when you are having trouble organizing your own writing. The process involves writing down the main idea of each paragraph on a separate piece of paper– see our short video . For the purposes of writing an abstract, try grouping the main ideas of each section of the paper into a single sentence. Practice grouping ideas using webbing or color coding .

For a scientific paper, you may have sections titled Purpose, Methods, Results, and Discussion. Each one of these sections will be longer than one paragraph, but each is grouped around a central idea. Use reverse outlining to discover the central idea in each section and then distill these ideas into one statement.

Cut and paste:

To create a first draft of an abstract of your own work, you can read through the entire paper and cut and paste sentences that capture key passages. This technique is useful for social science research with findings that cannot be encapsulated by neat numbers or concrete results. A well-written humanities draft will have a clear and direct thesis statement and informative topic sentences for paragraphs or sections. Isolate these sentences in a separate document and work on revising them into a unified paragraph.

If you are abstracting someone else’s writing

When abstracting something you have not written, you cannot summarize key ideas just by cutting and pasting. Instead, you must determine what a prospective reader would want to know about the work. There are a few techniques that will help you in this process:

Identify key terms:

Search through the entire document for key terms that identify the purpose, scope, and methods of the work. Pay close attention to the Introduction (or Purpose) and the Conclusion (or Discussion). These sections should contain all the main ideas and key terms in the paper. When writing the abstract, be sure to incorporate the key terms.

Highlight key phrases and sentences:

Instead of cutting and pasting the actual words, try highlighting sentences or phrases that appear to be central to the work. Then, in a separate document, rewrite the sentences and phrases in your own words.

Don’t look back:

After reading the entire work, put it aside and write a paragraph about the work without referring to it. In the first draft, you may not remember all the key terms or the results, but you will remember what the main point of the work was. Remember not to include any information you did not get from the work being abstracted.

Revise, revise, revise

No matter what type of abstract you are writing, or whether you are abstracting your own work or someone else’s, the most important step in writing an abstract is to revise early and often. When revising, delete all extraneous words and incorporate meaningful and powerful words. The idea is to be as clear and complete as possible in the shortest possible amount of space. The Word Count feature of Microsoft Word can help you keep track of how long your abstract is and help you hit your target length.

Example 1: Humanities abstract

Kenneth Tait Andrews, “‘Freedom is a constant struggle’: The dynamics and consequences of the Mississippi Civil Rights Movement, 1960-1984” Ph.D. State University of New York at Stony Brook, 1997 DAI-A 59/02, p. 620, Aug 1998

This dissertation examines the impacts of social movements through a multi-layered study of the Mississippi Civil Rights Movement from its peak in the early 1960s through the early 1980s. By examining this historically important case, I clarify the process by which movements transform social structures and the constraints movements face when they try to do so. The time period studied includes the expansion of voting rights and gains in black political power, the desegregation of public schools and the emergence of white-flight academies, and the rise and fall of federal anti-poverty programs. I use two major research strategies: (1) a quantitative analysis of county-level data and (2) three case studies. Data have been collected from archives, interviews, newspapers, and published reports. This dissertation challenges the argument that movements are inconsequential. Some view federal agencies, courts, political parties, or economic elites as the agents driving institutional change, but typically these groups acted in response to the leverage brought to bear by the civil rights movement. The Mississippi movement attempted to forge independent structures for sustaining challenges to local inequities and injustices. By propelling change in an array of local institutions, movement infrastructures had an enduring legacy in Mississippi.

Now let’s break down this abstract into its component parts to see how the author has distilled his entire dissertation into a ~200 word abstract.

What the dissertation does This dissertation examines the impacts of social movements through a multi-layered study of the Mississippi Civil Rights Movement from its peak in the early 1960s through the early 1980s. By examining this historically important case, I clarify the process by which movements transform social structures and the constraints movements face when they try to do so.

How the dissertation does it The time period studied in this dissertation includes the expansion of voting rights and gains in black political power, the desegregation of public schools and the emergence of white-flight academies, and the rise and fall of federal anti-poverty programs. I use two major research strategies: (1) a quantitative analysis of county-level data and (2) three case studies.

What materials are used Data have been collected from archives, interviews, newspapers, and published reports.

Conclusion This dissertation challenges the argument that movements are inconsequential. Some view federal agencies, courts, political parties, or economic elites as the agents driving institutional change, but typically these groups acted in response to movement demands and the leverage brought to bear by the civil rights movement. The Mississippi movement attempted to forge independent structures for sustaining challenges to local inequities and injustices. By propelling change in an array of local institutions, movement infrastructures had an enduring legacy in Mississippi.

Keywords social movements Civil Rights Movement Mississippi voting rights desegregation

Example 2: Science Abstract

Luis Lehner, “Gravitational radiation from black hole spacetimes” Ph.D. University of Pittsburgh, 1998 DAI-B 59/06, p. 2797, Dec 1998

The problem of detecting gravitational radiation is receiving considerable attention with the construction of new detectors in the United States, Europe, and Japan. The theoretical modeling of the wave forms that would be produced in particular systems will expedite the search for and analysis of detected signals. The characteristic formulation of GR is implemented to obtain an algorithm capable of evolving black holes in 3D asymptotically flat spacetimes. Using compactification techniques, future null infinity is included in the evolved region, which enables the unambiguous calculation of the radiation produced by some compact source. A module to calculate the waveforms is constructed and included in the evolution algorithm. This code is shown to be second-order convergent and to handle highly non-linear spacetimes. In particular, we have shown that the code can handle spacetimes whose radiation is equivalent to a galaxy converting its whole mass into gravitational radiation in one second. We further use the characteristic formulation to treat the region close to the singularity in black hole spacetimes. The code carefully excises a region surrounding the singularity and accurately evolves generic black hole spacetimes with apparently unlimited stability.

This science abstract covers much of the same ground as the humanities one, but it asks slightly different questions.

Why do this study The problem of detecting gravitational radiation is receiving considerable attention with the construction of new detectors in the United States, Europe, and Japan. The theoretical modeling of the wave forms that would be produced in particular systems will expedite the search and analysis of the detected signals.

What the study does The characteristic formulation of GR is implemented to obtain an algorithm capable of evolving black holes in 3D asymptotically flat spacetimes. Using compactification techniques, future null infinity is included in the evolved region, which enables the unambiguous calculation of the radiation produced by some compact source. A module to calculate the waveforms is constructed and included in the evolution algorithm.

Results This code is shown to be second-order convergent and to handle highly non-linear spacetimes. In particular, we have shown that the code can handle spacetimes whose radiation is equivalent to a galaxy converting its whole mass into gravitational radiation in one second. We further use the characteristic formulation to treat the region close to the singularity in black hole spacetimes. The code carefully excises a region surrounding the singularity and accurately evolves generic black hole spacetimes with apparently unlimited stability.

Keywords gravitational radiation (GR) spacetimes black holes

Works consulted

We consulted these works while writing this handout. This is not a comprehensive list of resources on the handout’s topic, and we encourage you to do your own research to find additional publications. Please do not use this list as a model for the format of your own reference list, as it may not match the citation style you are using. For guidance on formatting citations, please see the UNC Libraries citation tutorial . We revise these tips periodically and welcome feedback.

Belcher, Wendy Laura. 2009. Writing Your Journal Article in Twelve Weeks: A Guide to Academic Publishing Success. Thousand Oaks, CA: Sage Press.

Koopman, Philip. 1997. “How to Write an Abstract.” Carnegie Mellon University. October 1997. http://users.ece.cmu.edu/~koopman/essays/abstract.html .

Lancaster, F.W. 2003. Indexing And Abstracting in Theory and Practice , 3rd ed. London: Facet Publishing.

You may reproduce it for non-commercial use if you use the entire handout and attribute the source: The Writing Center, University of North Carolina at Chapel Hill

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The Dissertation Abstract: 101

How to write a clear & concise abstract (with examples).

By:   Madeline Fink (MSc) Reviewed By: Derek Jansen (MBA)   | June 2020

So, you’ve (finally) finished your thesis or dissertation or thesis. Now it’s time to write up your abstract (sometimes also called the executive summary). If you’re here, chances are you’re not quite sure what you need to cover in this section, or how to go about writing it. Fear not – we’ll explain it all in plain language , step by step , with clear examples .

Overview: The Dissertation/Thesis Abstract

• What exactly is a dissertation (or thesis) abstract
• What’s the purpose and function of the abstract
• Why is the abstract so important
• How to write a high-quality dissertation abstract
• Example/sample of a quality abstract
• Quick tips to write a high-quality dissertation abstract

What is an abstract?

Simply put, the abstract in a dissertation or thesis is a short (but well structured) summary that outlines the most important points of your research (i.e. the key takeaways). The abstract is usually 1 paragraph or about 300-500 words long (about one page), but but this can vary between universities.

A quick note regarding terminology – strictly speaking, an abstract and an executive summary are two different things when it comes to academic publications. Typically, an abstract only states what the research will be about, but doesn’t explore the findings – whereas an executive summary covers both . However, in the context of a dissertation or thesis, the abstract usually covers both, providing a summary of the full project.

In terms of content, a good dissertation abstract usually covers the following points:

• The purpose of the research (what’s it about and why’s that important)
• The methodology (how you carried out the research)
• The key research findings (what answers you found)
• The implications of these findings (what these answers mean)

We’ll explain each of these in more detail a little later in this post. Buckle up.

What’s the purpose of the abstract?

A dissertation abstract has two main functions:

The first purpose is to  inform potential readers  of the main idea of your research without them having to read your entire piece of work. Specifically, it needs to communicate what your research is about (what were you trying to find out) and what your findings were . When readers are deciding whether to read your dissertation or thesis, the abstract is the first part they’ll consider. 

The second purpose of the abstract is to  inform search engines and dissertation databases  as they index your dissertation or thesis. The keywords and phrases in your abstract (as well as your keyword list) will often be used by these search engines to categorize your work and make it accessible to users. 

Simply put, your abstract is your shopfront display window – it’s what passers-by (both human and digital) will look at before deciding to step inside. 

Why’s it so important?

The short answer – because most people don’t have time to read your full dissertation or thesis! Time is money, after all…

If you think back to when you undertook your literature review , you’ll quickly realise just how important abstracts are! Researchers reviewing the literature on any given topic face a mountain of reading, so they need to optimise their approach. A good dissertation abstract gives the reader a “TLDR” version of your work – it helps them decide whether to continue to read it in its entirety. So, your abstract, as your shopfront display window, needs to “sell” your research to time-poor readers.

You might be thinking, “but I don’t plan to publish my dissertation”. Even so, you still need to provide an impactful abstract for your markers. Your ability to concisely summarise your work is one of the things they’re assessing, so it’s vital to invest time and effort into crafting an enticing shop window.  

A good abstract also has an added purpose for grad students . As a freshly minted graduate, your dissertation or thesis is often your most significant professional accomplishment and highlights where your unique expertise lies. Potential employers who want to know about this expertise are likely to only read the abstract (as opposed to reading your entire document) – so it needs to be good!

Think about it this way – if your thesis or dissertation were a book, then the abstract would be the blurb on the back cover. For better or worse, readers will absolutely judge your book by its cover .

How to write your abstract

As we touched on earlier, your abstract should cover four important aspects of your research: the purpose , methodology , findings , and implications . Therefore, the structure of your dissertation or thesis abstract needs to reflect these four essentials, in the same order.  Let’s take a closer look at each of them, step by step:

Step 1: Describe the purpose and value of your research

Here you need to concisely explain the purpose and value of your research. In other words, you need to explain what your research set out to discover and why that’s important. When stating the purpose of research, you need to clearly discuss the following:

• What were your research aims and research questions ?
• Why were these aims and questions important?

It’s essential to make this section extremely clear, concise and convincing . As the opening section, this is where you’ll “hook” your reader (marker) in and get them interested in your project. If you don’t put in the effort here, you’ll likely lose their interest.

Step 2: Briefly outline your study’s methodology

In this part of your abstract, you need to very briefly explain how you went about answering your research questions . In other words, what research design and methodology you adopted in your research. Some important questions to address here include:

• Did you take a qualitative or quantitative approach ?
• Who/what did your sample consist of?
• How did you collect your data?
• How did you analyse your data?

Simply put, this section needs to address the “ how ” of your research. It doesn’t need to be lengthy (this is just a summary, after all), but it should clearly address the four questions above.

Need a helping hand?

Step 3: Present your key findings

Next, you need to briefly highlight the key findings . Your research likely produced a wealth of data and findings, so there may be a temptation to ramble here. However, this section is just about the key findings – in other words, the answers to the original questions that you set out to address.

Again, brevity and clarity are important here. You need to concisely present the most important findings for your reader.

Step 4: Describe the implications of your research

Have you ever found yourself reading through a large report, struggling to figure out what all the findings mean in terms of the bigger picture? Well, that’s the purpose of the implications section – to highlight the “so what?” of your research. 

In this part of your abstract, you should address the following questions:

• What is the impact of your research findings on the industry /field investigated? In other words, what’s the impact on the “real world”. 
• What is the impact of your findings on the existing body of knowledge ? For example, do they support the existing research?
• What might your findings mean for future research conducted on your topic?

If you include these four essential ingredients in your dissertation abstract, you’ll be on headed in a good direction.

Example: Dissertation/thesis abstract

Here is an example of an abstract from a master’s thesis, with the purpose , methods , findings , and implications colour coded.

The U.S. citizenship application process is a legal and symbolic journey shaped by many cultural processes. This research project aims to bring to light the experiences of immigrants and citizenship applicants living in Dallas, Texas, to promote a better understanding of Dallas’ increasingly diverse population. Additionally, the purpose of this project is to provide insights to a specific client, the office of Dallas Welcoming Communities and Immigrant Affairs, about Dallas’ lawful permanent residents who are eligible for citizenship and their reasons for pursuing citizenship status . The data for this project was collected through observation at various citizenship workshops and community events, as well as through semi-structured interviews with 14 U.S. citizenship applicants . Reasons for applying for U.S. citizenship discussed in this project include a desire for membership in U.S. society, access to better educational and economic opportunities, improved ease of travel and the desire to vote. Barriers to the citizenship process discussed in this project include the amount of time one must dedicate to the application, lack of clear knowledge about the process and the financial cost of the application. Other themes include the effects of capital on applicant’s experience with the citizenship process, symbolic meanings of citizenship, transnationalism and ideas of deserving and undeserving surrounding the issues of residency and U.S. citizenship. These findings indicate the need for educational resources and mentorship for Dallas-area residents applying for U.S. citizenship, as well as a need for local government programs that foster a sense of community among citizenship applicants and their neighbours.

Practical tips for writing your abstract

When crafting the abstract for your dissertation or thesis, the most powerful technique you can use is to try and put yourself in the shoes of a potential reader. Assume the reader is not an expert in the field, but is interested in the research area. In other words, write for the intelligent layman, not for the seasoned topic expert. 

Start by trying to answer the question “why should I read this dissertation?”

Remember the WWHS.

Make sure you include the  what , why ,  how , and  so what  of your research in your abstract:

• What you studied (who and where are included in this part)
• Why the topic was important
• How you designed your study (i.e. your research methodology)
• So what were the big findings and implications of your research

Keep it simple.

Use terminology appropriate to your field of study, but don’t overload your abstract with big words and jargon that cloud the meaning and make your writing difficult to digest. A good abstract should appeal to all levels of potential readers and should be a (relatively) easy read. Remember, you need to write for the intelligent layman.

Be specific.

When writing your abstract, clearly outline your most important findings and insights and don’t worry about “giving away” too much about your research – there’s no need to withhold information. This is the one way your abstract is not like a blurb on the back of a book – the reader should be able to clearly understand the key takeaways of your thesis or dissertation after reading the abstract. Of course, if they then want more detail, they need to step into the restaurant and try out the menu.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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How to Write an Abstract for a Research Paper | Examples

What is a research paper abstract?

Research paper abstracts summarize your study quickly and succinctly to journal editors and researchers and prompt them to read further. But with the ubiquity of online publication databases, writing a compelling abstract is even more important today than it was in the days of bound paper manuscripts.

Abstracts exist to “sell”  your work, and they could thus be compared to the “executive summary” of a business resume: an official briefing on what is most important about your research. Or the “gist” of your research. With the majority of academic transactions being conducted online, this means that you have even less time to impress readers–and increased competition in terms of other abstracts out there to read.

The APCI (Academic Publishing and Conferences International) notes that there are  12 questions or “points” considered in the selection process  for journals and conferences and stresses the importance of having an abstract that ticks all of these boxes. Because it is often the ONLY chance you have to convince readers to keep reading, it is important that you spend time and energy crafting an abstract that faithfully represents the central parts of your study and captivates your audience.

With that in mind, follow these suggestions when structuring and writing your abstract, and learn how exactly to put these ideas into a solid abstract that will captivate your target readers.

Before Writing Your Abstract

How long should an abstract be.

All abstracts are written with the same essential objective: to give a summary of your study. But there are two basic styles of abstract: descriptive and informative . Here is a brief delineation of the two:

Of the two types of abstracts, informative abstracts are much more common, and they are widely used for submission to journals and conferences. Informative abstracts apply to lengthier and more technical research and are common in the sciences, engineering, and psychology, while descriptive abstracts are more likely used in humanities and social science papers. The best method of determining which abstract type you need to use is to follow the instructions for journal submissions and to read as many other published articles in those journals as possible.

Research Abstract Guidelines and Requirements

As any article about research writing will tell you, authors must always closely follow the specific guidelines and requirements indicated in the Guide for Authors section of their target journal’s website. The same kind of adherence to conventions should be applied to journal publications, for consideration at a conference, and even when completing a class assignment.

Each publisher has particular demands when it comes to formatting and structure. Here are some common questions addressed in the journal guidelines:

• Is there a maximum or minimum word/character length?
• What are the style and formatting requirements?
• What is the appropriate abstract type?
• Are there any specific content or organization rules that apply?

There are of course other rules to consider when composing a research paper abstract. But if you follow the stated rules the first time you submit your manuscript, you can avoid your work being thrown in the “circular file” right off the bat.

Identify Your Target Readership

The main purpose of your abstract is to lead researchers to the full text of your research paper. In scientific journals, abstracts let readers decide whether the research discussed is relevant to their own interests or study. Abstracts also help readers understand your main argument quickly. Consider these questions as you write your abstract:

• Are other academics in your field the main target of your study?
• Will your study perhaps be useful to members of the general public?
• Do your study results include the wider implications presented in the abstract?

Outlining and Writing Your Abstract

What to include in an abstract.

Just as your  research paper title  should cover as much ground as possible in a few short words, your abstract must cover  all  parts of your study in order to fully explain your paper and research. Because it must accomplish this task in the space of only a few hundred words, it is important not to include ambiguous references or phrases that will confuse the reader or mislead them about the content and objectives of your research. Follow these  dos  and  don’ts  when it comes to what kind of writing to include:

• Avoid acronyms or abbreviations since these will need to be explained in order to make sense to the reader, which takes up valuable abstract space. Instead, explain these terms in the Introduction section of the main text.
• Only use references to people or other works if they are well-known. Otherwise, avoid referencing anything outside of your study in the abstract.
• Never include tables, figures, sources, or long quotations in your abstract; you will have plenty of time to present and refer to these in the body of your paper.

Use keywords in your abstract to focus your topic

A vital search tool is the research paper keywords section, which lists the most relevant terms directly underneath the abstract. Think of these keywords as the “tubes” that readers will seek and enter—via queries on databases and search engines—to ultimately land at their destination, which is your paper. Your abstract keywords should thus be words that are commonly used in searches but should also be highly relevant to your work and found in the text of your abstract. Include 5 to 10 important words or short phrases central to your research in both the abstract and the keywords section.

For example, if you are writing a paper on the prevalence of obesity among lower classes that crosses international boundaries, you should include terms like “obesity,” “prevalence,” “international,” “lower classes,” and “cross-cultural.” These are terms that should net a wide array of people interested in your topic of study. Look at our nine rules for choosing keywords for your research paper if you need more input on this.

Research Paper Abstract Structure

As mentioned above, the abstract (especially the informative abstract) acts as a surrogate or synopsis of your research paper, doing almost as much work as the thousands of words that follow it in the body of the main text. In the hard sciences and most social sciences, the abstract includes the following sections and organizational schema.

Each section is quite compact—only a single sentence or two, although there is room for expansion if one element or statement is particularly interesting or compelling. As the abstract is almost always one long paragraph, the individual sections should naturally merge into one another to create a holistic effect. Use the following as a checklist to ensure that you have included all of the necessary content in your abstract.

1) Identify your purpose and motivation

So your research is about rabies in Brazilian squirrels. Why is this important? You should start your abstract by explaining why people should care about this study—why is it significant to your field and perhaps to the wider world? And what is the exact purpose of your study; what are you trying to achieve? Start by answering the following questions:

• What made you decide to do this study or project?
• Why is this study important to your field or to the lay reader?
• Why should someone read your entire article?

In summary, the first section of your abstract should include the importance of the research and its impact on related research fields or on the wider scientific domain.

2) Explain the research problem you are addressing

Stating the research problem that your study addresses is the corollary to why your specific study is important and necessary. For instance, even if the issue of “rabies in Brazilian squirrels” is important, what is the problem—the “missing piece of the puzzle”—that your study helps resolve?

You can combine the problem with the motivation section, but from a perspective of organization and clarity, it is best to separate the two. Here are some precise questions to address:

• What is your research trying to better understand or what problem is it trying to solve?
• What is the scope of your study—does it try to explain something general or specific?
• What is your central claim or argument?

3) Discuss your research approach

Your specific study approach is detailed in the Methods and Materials section .  You have already established the importance of the research, your motivation for studying this issue, and the specific problem your paper addresses. Now you need to discuss  how  you solved or made progress on this problem—how you conducted your research. If your study includes your own work or that of your team, describe that here. If in your paper you reviewed the work of others, explain this here. Did you use analytic models? A simulation? A double-blind study? A case study? You are basically showing the reader the internal engine of your research machine and how it functioned in the study. Be sure to:

• Detail your research—include methods/type of the study, your variables, and the extent of the work
• Briefly present evidence to support your claim
• Highlight your most important sources

4) Briefly summarize your results

Here you will give an overview of the outcome of your study. Avoid using too many vague qualitative terms (e.g, “very,” “small,” or “tremendous”) and try to use at least some quantitative terms (i.e., percentages, figures, numbers). Save your qualitative language for the conclusion statement. Answer questions like these:

• What did your study yield in concrete terms (e.g., trends, figures, correlation between phenomena)?
• How did your results compare to your hypothesis? Was the study successful?
• Where there any highly unexpected outcomes or were they all largely predicted?

5) State your conclusion

In the last section of your abstract, you will give a statement about the implications and  limitations of the study . Be sure to connect this statement closely to your results and not the area of study in general. Are the results of this study going to shake up the scientific world? Will they impact how people see “Brazilian squirrels”? Or are the implications minor? Try not to boast about your study or present its impact as  too  far-reaching, as researchers and journals will tend to be skeptical of bold claims in scientific papers. Answer one of these questions:

• What are the exact effects of these results on my field? On the wider world?
• What other kind of study would yield further solutions to problems?
• What other information is needed to expand knowledge in this area?

After Completing the First Draft of Your Abstract

Revise your abstract.

The abstract, like any piece of academic writing, should be revised before being considered complete. Check it for  grammatical and spelling errors  and make sure it is formatted properly.

Get feedback from a peer

Getting a fresh set of eyes to review your abstract is a great way to find out whether you’ve summarized your research well. Find a reader who understands research papers but is not an expert in this field or is not affiliated with your study. Ask your reader to summarize what your study is about (including all key points of each section). This should tell you if you have communicated your key points clearly.

In addition to research peers, consider consulting with a professor or even a specialist or generalist writing center consultant about your abstract. Use any resource that helps you see your work from another perspective.

Consider getting professional editing and proofreading

While peer feedback is quite important to ensure the effectiveness of your abstract content, it may be a good idea to find an academic editor  to fix mistakes in grammar, spelling, mechanics, style, or formatting. The presence of basic errors in the abstract may not affect your content, but it might dissuade someone from reading your entire study. Wordvice provides English editing services that both correct objective errors and enhance the readability and impact of your work.

Additional Abstract Rules and Guidelines

Write your abstract after completing your paper.

Although the abstract goes at the beginning of your manuscript, it does not merely introduce your research topic (that is the job of the title), but rather summarizes your entire paper. Writing the abstract last will ensure that it is complete and consistent with the findings and statements in your paper.

Keep your content in the correct order

Both questions and answers should be organized in a standard and familiar way to make the content easier for readers to absorb. Ideally, it should mimic the overall format of your essay and the classic “introduction,” “body,” and “conclusion” form, even if the parts are not neatly divided as such.

Write the abstract from scratch

Because the abstract is a self-contained piece of writing viewed separately from the body of the paper, you should write it separately as well. Never copy and paste direct quotes from the paper and avoid paraphrasing sentences in the paper. Using new vocabulary and phrases will keep your abstract interesting and free of redundancies while conserving space.

Don’t include too many details in the abstract

Again, the density of your abstract makes it incompatible with including specific points other than possibly names or locations. You can make references to terms, but do not explain or define them in the abstract. Try to strike a balance between being specific to your study and presenting a relatively broad overview of your work.

Wordvice Resources

If you think your abstract is fine now but you need input on abstract writing or require English editing services (including paper editing ), then head over to the Wordvice academic resources page, where you will find many more articles, for example on writing the Results , Methods , and Discussion sections of your manuscript, on choosing a title for your paper , or on how to finalize your journal submission with a strong cover letter .    

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A Practical Guide to Writing Quantitative and Qualitative Research Questions and Hypotheses in Scholarly Articles

Edward barroga.

1 Department of General Education, Graduate School of Nursing Science, St. Luke’s International University, Tokyo, Japan.

Glafera Janet Matanguihan

2 Department of Biological Sciences, Messiah University, Mechanicsburg, PA, USA.

The development of research questions and the subsequent hypotheses are prerequisites to defining the main research purpose and specific objectives of a study. Consequently, these objectives determine the study design and research outcome. The development of research questions is a process based on knowledge of current trends, cutting-edge studies, and technological advances in the research field. Excellent research questions are focused and require a comprehensive literature search and in-depth understanding of the problem being investigated. Initially, research questions may be written as descriptive questions which could be developed into inferential questions. These questions must be specific and concise to provide a clear foundation for developing hypotheses. Hypotheses are more formal predictions about the research outcomes. These specify the possible results that may or may not be expected regarding the relationship between groups. Thus, research questions and hypotheses clarify the main purpose and specific objectives of the study, which in turn dictate the design of the study, its direction, and outcome. Studies developed from good research questions and hypotheses will have trustworthy outcomes with wide-ranging social and health implications.

INTRODUCTION

Scientific research is usually initiated by posing evidenced-based research questions which are then explicitly restated as hypotheses. 1 , 2 The hypotheses provide directions to guide the study, solutions, explanations, and expected results. 3 , 4 Both research questions and hypotheses are essentially formulated based on conventional theories and real-world processes, which allow the inception of novel studies and the ethical testing of ideas. 5 , 6

It is crucial to have knowledge of both quantitative and qualitative research 2 as both types of research involve writing research questions and hypotheses. 7 However, these crucial elements of research are sometimes overlooked; if not overlooked, then framed without the forethought and meticulous attention it needs. Planning and careful consideration are needed when developing quantitative or qualitative research, particularly when conceptualizing research questions and hypotheses. 4

There is a continuing need to support researchers in the creation of innovative research questions and hypotheses, as well as for journal articles that carefully review these elements. 1 When research questions and hypotheses are not carefully thought of, unethical studies and poor outcomes usually ensue. Carefully formulated research questions and hypotheses define well-founded objectives, which in turn determine the appropriate design, course, and outcome of the study. This article then aims to discuss in detail the various aspects of crafting research questions and hypotheses, with the goal of guiding researchers as they develop their own. Examples from the authors and peer-reviewed scientific articles in the healthcare field are provided to illustrate key points.

DEFINITIONS AND RELATIONSHIP OF RESEARCH QUESTIONS AND HYPOTHESES

A research question is what a study aims to answer after data analysis and interpretation. The answer is written in length in the discussion section of the paper. Thus, the research question gives a preview of the different parts and variables of the study meant to address the problem posed in the research question. 1 An excellent research question clarifies the research writing while facilitating understanding of the research topic, objective, scope, and limitations of the study. 5

On the other hand, a research hypothesis is an educated statement of an expected outcome. This statement is based on background research and current knowledge. 8 , 9 The research hypothesis makes a specific prediction about a new phenomenon 10 or a formal statement on the expected relationship between an independent variable and a dependent variable. 3 , 11 It provides a tentative answer to the research question to be tested or explored. 4

Hypotheses employ reasoning to predict a theory-based outcome. 10 These can also be developed from theories by focusing on components of theories that have not yet been observed. 10 The validity of hypotheses is often based on the testability of the prediction made in a reproducible experiment. 8

Conversely, hypotheses can also be rephrased as research questions. Several hypotheses based on existing theories and knowledge may be needed to answer a research question. Developing ethical research questions and hypotheses creates a research design that has logical relationships among variables. These relationships serve as a solid foundation for the conduct of the study. 4 , 11 Haphazardly constructed research questions can result in poorly formulated hypotheses and improper study designs, leading to unreliable results. Thus, the formulations of relevant research questions and verifiable hypotheses are crucial when beginning research. 12

CHARACTERISTICS OF GOOD RESEARCH QUESTIONS AND HYPOTHESES

Excellent research questions are specific and focused. These integrate collective data and observations to confirm or refute the subsequent hypotheses. Well-constructed hypotheses are based on previous reports and verify the research context. These are realistic, in-depth, sufficiently complex, and reproducible. More importantly, these hypotheses can be addressed and tested. 13

There are several characteristics of well-developed hypotheses. Good hypotheses are 1) empirically testable 7 , 10 , 11 , 13 ; 2) backed by preliminary evidence 9 ; 3) testable by ethical research 7 , 9 ; 4) based on original ideas 9 ; 5) have evidenced-based logical reasoning 10 ; and 6) can be predicted. 11 Good hypotheses can infer ethical and positive implications, indicating the presence of a relationship or effect relevant to the research theme. 7 , 11 These are initially developed from a general theory and branch into specific hypotheses by deductive reasoning. In the absence of a theory to base the hypotheses, inductive reasoning based on specific observations or findings form more general hypotheses. 10

TYPES OF RESEARCH QUESTIONS AND HYPOTHESES

Research questions and hypotheses are developed according to the type of research, which can be broadly classified into quantitative and qualitative research. We provide a summary of the types of research questions and hypotheses under quantitative and qualitative research categories in Table 1 .

Research questions in quantitative research

In quantitative research, research questions inquire about the relationships among variables being investigated and are usually framed at the start of the study. These are precise and typically linked to the subject population, dependent and independent variables, and research design. 1 Research questions may also attempt to describe the behavior of a population in relation to one or more variables, or describe the characteristics of variables to be measured ( descriptive research questions ). 1 , 5 , 14 These questions may also aim to discover differences between groups within the context of an outcome variable ( comparative research questions ), 1 , 5 , 14 or elucidate trends and interactions among variables ( relationship research questions ). 1 , 5 We provide examples of descriptive, comparative, and relationship research questions in quantitative research in Table 2 .

Hypotheses in quantitative research

In quantitative research, hypotheses predict the expected relationships among variables. 15 Relationships among variables that can be predicted include 1) between a single dependent variable and a single independent variable ( simple hypothesis ) or 2) between two or more independent and dependent variables ( complex hypothesis ). 4 , 11 Hypotheses may also specify the expected direction to be followed and imply an intellectual commitment to a particular outcome ( directional hypothesis ) 4 . On the other hand, hypotheses may not predict the exact direction and are used in the absence of a theory, or when findings contradict previous studies ( non-directional hypothesis ). 4 In addition, hypotheses can 1) define interdependency between variables ( associative hypothesis ), 4 2) propose an effect on the dependent variable from manipulation of the independent variable ( causal hypothesis ), 4 3) state a negative relationship between two variables ( null hypothesis ), 4 , 11 , 15 4) replace the working hypothesis if rejected ( alternative hypothesis ), 15 explain the relationship of phenomena to possibly generate a theory ( working hypothesis ), 11 5) involve quantifiable variables that can be tested statistically ( statistical hypothesis ), 11 6) or express a relationship whose interlinks can be verified logically ( logical hypothesis ). 11 We provide examples of simple, complex, directional, non-directional, associative, causal, null, alternative, working, statistical, and logical hypotheses in quantitative research, as well as the definition of quantitative hypothesis-testing research in Table 3 .

Research questions in qualitative research

Unlike research questions in quantitative research, research questions in qualitative research are usually continuously reviewed and reformulated. The central question and associated subquestions are stated more than the hypotheses. 15 The central question broadly explores a complex set of factors surrounding the central phenomenon, aiming to present the varied perspectives of participants. 15

There are varied goals for which qualitative research questions are developed. These questions can function in several ways, such as to 1) identify and describe existing conditions ( contextual research question s); 2) describe a phenomenon ( descriptive research questions ); 3) assess the effectiveness of existing methods, protocols, theories, or procedures ( evaluation research questions ); 4) examine a phenomenon or analyze the reasons or relationships between subjects or phenomena ( explanatory research questions ); or 5) focus on unknown aspects of a particular topic ( exploratory research questions ). 5 In addition, some qualitative research questions provide new ideas for the development of theories and actions ( generative research questions ) or advance specific ideologies of a position ( ideological research questions ). 1 Other qualitative research questions may build on a body of existing literature and become working guidelines ( ethnographic research questions ). Research questions may also be broadly stated without specific reference to the existing literature or a typology of questions ( phenomenological research questions ), may be directed towards generating a theory of some process ( grounded theory questions ), or may address a description of the case and the emerging themes ( qualitative case study questions ). 15 We provide examples of contextual, descriptive, evaluation, explanatory, exploratory, generative, ideological, ethnographic, phenomenological, grounded theory, and qualitative case study research questions in qualitative research in Table 4 , and the definition of qualitative hypothesis-generating research in Table 5 .

Qualitative studies usually pose at least one central research question and several subquestions starting with How or What . These research questions use exploratory verbs such as explore or describe . These also focus on one central phenomenon of interest, and may mention the participants and research site. 15

Hypotheses in qualitative research

Hypotheses in qualitative research are stated in the form of a clear statement concerning the problem to be investigated. Unlike in quantitative research where hypotheses are usually developed to be tested, qualitative research can lead to both hypothesis-testing and hypothesis-generating outcomes. 2 When studies require both quantitative and qualitative research questions, this suggests an integrative process between both research methods wherein a single mixed-methods research question can be developed. 1

FRAMEWORKS FOR DEVELOPING RESEARCH QUESTIONS AND HYPOTHESES

Research questions followed by hypotheses should be developed before the start of the study. 1 , 12 , 14 It is crucial to develop feasible research questions on a topic that is interesting to both the researcher and the scientific community. This can be achieved by a meticulous review of previous and current studies to establish a novel topic. Specific areas are subsequently focused on to generate ethical research questions. The relevance of the research questions is evaluated in terms of clarity of the resulting data, specificity of the methodology, objectivity of the outcome, depth of the research, and impact of the study. 1 , 5 These aspects constitute the FINER criteria (i.e., Feasible, Interesting, Novel, Ethical, and Relevant). 1 Clarity and effectiveness are achieved if research questions meet the FINER criteria. In addition to the FINER criteria, Ratan et al. described focus, complexity, novelty, feasibility, and measurability for evaluating the effectiveness of research questions. 14

The PICOT and PEO frameworks are also used when developing research questions. 1 The following elements are addressed in these frameworks, PICOT: P-population/patients/problem, I-intervention or indicator being studied, C-comparison group, O-outcome of interest, and T-timeframe of the study; PEO: P-population being studied, E-exposure to preexisting conditions, and O-outcome of interest. 1 Research questions are also considered good if these meet the “FINERMAPS” framework: Feasible, Interesting, Novel, Ethical, Relevant, Manageable, Appropriate, Potential value/publishable, and Systematic. 14

As we indicated earlier, research questions and hypotheses that are not carefully formulated result in unethical studies or poor outcomes. To illustrate this, we provide some examples of ambiguous research question and hypotheses that result in unclear and weak research objectives in quantitative research ( Table 6 ) 16 and qualitative research ( Table 7 ) 17 , and how to transform these ambiguous research question(s) and hypothesis(es) into clear and good statements.

a These statements were composed for comparison and illustrative purposes only.

b These statements are direct quotes from Higashihara and Horiuchi. 16

a This statement is a direct quote from Shimoda et al. 17

The other statements were composed for comparison and illustrative purposes only.

CONSTRUCTING RESEARCH QUESTIONS AND HYPOTHESES

To construct effective research questions and hypotheses, it is very important to 1) clarify the background and 2) identify the research problem at the outset of the research, within a specific timeframe. 9 Then, 3) review or conduct preliminary research to collect all available knowledge about the possible research questions by studying theories and previous studies. 18 Afterwards, 4) construct research questions to investigate the research problem. Identify variables to be accessed from the research questions 4 and make operational definitions of constructs from the research problem and questions. Thereafter, 5) construct specific deductive or inductive predictions in the form of hypotheses. 4 Finally, 6) state the study aims . This general flow for constructing effective research questions and hypotheses prior to conducting research is shown in Fig. 1 .

Research questions are used more frequently in qualitative research than objectives or hypotheses. 3 These questions seek to discover, understand, explore or describe experiences by asking “What” or “How.” The questions are open-ended to elicit a description rather than to relate variables or compare groups. The questions are continually reviewed, reformulated, and changed during the qualitative study. 3 Research questions are also used more frequently in survey projects than hypotheses in experiments in quantitative research to compare variables and their relationships.

Hypotheses are constructed based on the variables identified and as an if-then statement, following the template, ‘If a specific action is taken, then a certain outcome is expected.’ At this stage, some ideas regarding expectations from the research to be conducted must be drawn. 18 Then, the variables to be manipulated (independent) and influenced (dependent) are defined. 4 Thereafter, the hypothesis is stated and refined, and reproducible data tailored to the hypothesis are identified, collected, and analyzed. 4 The hypotheses must be testable and specific, 18 and should describe the variables and their relationships, the specific group being studied, and the predicted research outcome. 18 Hypotheses construction involves a testable proposition to be deduced from theory, and independent and dependent variables to be separated and measured separately. 3 Therefore, good hypotheses must be based on good research questions constructed at the start of a study or trial. 12

In summary, research questions are constructed after establishing the background of the study. Hypotheses are then developed based on the research questions. Thus, it is crucial to have excellent research questions to generate superior hypotheses. In turn, these would determine the research objectives and the design of the study, and ultimately, the outcome of the research. 12 Algorithms for building research questions and hypotheses are shown in Fig. 2 for quantitative research and in Fig. 3 for qualitative research.

EXAMPLES OF RESEARCH QUESTIONS FROM PUBLISHED ARTICLES

• EXAMPLE 1. Descriptive research question (quantitative research)
• - Presents research variables to be assessed (distinct phenotypes and subphenotypes)
• “BACKGROUND: Since COVID-19 was identified, its clinical and biological heterogeneity has been recognized. Identifying COVID-19 phenotypes might help guide basic, clinical, and translational research efforts.
• RESEARCH QUESTION: Does the clinical spectrum of patients with COVID-19 contain distinct phenotypes and subphenotypes? ” 19
• EXAMPLE 2. Relationship research question (quantitative research)
• - Shows interactions between dependent variable (static postural control) and independent variable (peripheral visual field loss)
• “Background: Integration of visual, vestibular, and proprioceptive sensations contributes to postural control. People with peripheral visual field loss have serious postural instability. However, the directional specificity of postural stability and sensory reweighting caused by gradual peripheral visual field loss remain unclear.
• Research question: What are the effects of peripheral visual field loss on static postural control ?” 20
• EXAMPLE 3. Comparative research question (quantitative research)
• - Clarifies the difference among groups with an outcome variable (patients enrolled in COMPERA with moderate PH or severe PH in COPD) and another group without the outcome variable (patients with idiopathic pulmonary arterial hypertension (IPAH))
• “BACKGROUND: Pulmonary hypertension (PH) in COPD is a poorly investigated clinical condition.
• RESEARCH QUESTION: Which factors determine the outcome of PH in COPD?
• STUDY DESIGN AND METHODS: We analyzed the characteristics and outcome of patients enrolled in the Comparative, Prospective Registry of Newly Initiated Therapies for Pulmonary Hypertension (COMPERA) with moderate or severe PH in COPD as defined during the 6th PH World Symposium who received medical therapy for PH and compared them with patients with idiopathic pulmonary arterial hypertension (IPAH) .” 21
• EXAMPLE 4. Exploratory research question (qualitative research)
• - Explores areas that have not been fully investigated (perspectives of families and children who receive care in clinic-based child obesity treatment) to have a deeper understanding of the research problem
• “Problem: Interventions for children with obesity lead to only modest improvements in BMI and long-term outcomes, and data are limited on the perspectives of families of children with obesity in clinic-based treatment. This scoping review seeks to answer the question: What is known about the perspectives of families and children who receive care in clinic-based child obesity treatment? This review aims to explore the scope of perspectives reported by families of children with obesity who have received individualized outpatient clinic-based obesity treatment.” 22
• EXAMPLE 5. Relationship research question (quantitative research)
• - Defines interactions between dependent variable (use of ankle strategies) and independent variable (changes in muscle tone)
• “Background: To maintain an upright standing posture against external disturbances, the human body mainly employs two types of postural control strategies: “ankle strategy” and “hip strategy.” While it has been reported that the magnitude of the disturbance alters the use of postural control strategies, it has not been elucidated how the level of muscle tone, one of the crucial parameters of bodily function, determines the use of each strategy. We have previously confirmed using forward dynamics simulations of human musculoskeletal models that an increased muscle tone promotes the use of ankle strategies. The objective of the present study was to experimentally evaluate a hypothesis: an increased muscle tone promotes the use of ankle strategies. Research question: Do changes in the muscle tone affect the use of ankle strategies ?” 23

EXAMPLES OF HYPOTHESES IN PUBLISHED ARTICLES

• EXAMPLE 1. Working hypothesis (quantitative research)
• - A hypothesis that is initially accepted for further research to produce a feasible theory
• “As fever may have benefit in shortening the duration of viral illness, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response when taken during the early stages of COVID-19 illness .” 24
• “In conclusion, it is plausible to hypothesize that the antipyretic efficacy of ibuprofen may be hindering the benefits of a fever response . The difference in perceived safety of these agents in COVID-19 illness could be related to the more potent efficacy to reduce fever with ibuprofen compared to acetaminophen. Compelling data on the benefit of fever warrant further research and review to determine when to treat or withhold ibuprofen for early stage fever for COVID-19 and other related viral illnesses .” 24
• EXAMPLE 2. Exploratory hypothesis (qualitative research)
• - Explores particular areas deeper to clarify subjective experience and develop a formal hypothesis potentially testable in a future quantitative approach
• “We hypothesized that when thinking about a past experience of help-seeking, a self distancing prompt would cause increased help-seeking intentions and more favorable help-seeking outcome expectations .” 25
• “Conclusion
• Although a priori hypotheses were not supported, further research is warranted as results indicate the potential for using self-distancing approaches to increasing help-seeking among some people with depressive symptomatology.” 25
• EXAMPLE 3. Hypothesis-generating research to establish a framework for hypothesis testing (qualitative research)
• “We hypothesize that compassionate care is beneficial for patients (better outcomes), healthcare systems and payers (lower costs), and healthcare providers (lower burnout). ” 26
• Compassionomics is the branch of knowledge and scientific study of the effects of compassionate healthcare. Our main hypotheses are that compassionate healthcare is beneficial for (1) patients, by improving clinical outcomes, (2) healthcare systems and payers, by supporting financial sustainability, and (3) HCPs, by lowering burnout and promoting resilience and well-being. The purpose of this paper is to establish a scientific framework for testing the hypotheses above . If these hypotheses are confirmed through rigorous research, compassionomics will belong in the science of evidence-based medicine, with major implications for all healthcare domains.” 26
• EXAMPLE 4. Statistical hypothesis (quantitative research)
• - An assumption is made about the relationship among several population characteristics ( gender differences in sociodemographic and clinical characteristics of adults with ADHD ). Validity is tested by statistical experiment or analysis ( chi-square test, Students t-test, and logistic regression analysis)
• “Our research investigated gender differences in sociodemographic and clinical characteristics of adults with ADHD in a Japanese clinical sample. Due to unique Japanese cultural ideals and expectations of women's behavior that are in opposition to ADHD symptoms, we hypothesized that women with ADHD experience more difficulties and present more dysfunctions than men . We tested the following hypotheses: first, women with ADHD have more comorbidities than men with ADHD; second, women with ADHD experience more social hardships than men, such as having less full-time employment and being more likely to be divorced.” 27
• “Statistical Analysis
• ( text omitted ) Between-gender comparisons were made using the chi-squared test for categorical variables and Students t-test for continuous variables…( text omitted ). A logistic regression analysis was performed for employment status, marital status, and comorbidity to evaluate the independent effects of gender on these dependent variables.” 27

EXAMPLES OF HYPOTHESIS AS WRITTEN IN PUBLISHED ARTICLES IN RELATION TO OTHER PARTS

• EXAMPLE 1. Background, hypotheses, and aims are provided
• “Pregnant women need skilled care during pregnancy and childbirth, but that skilled care is often delayed in some countries …( text omitted ). The focused antenatal care (FANC) model of WHO recommends that nurses provide information or counseling to all pregnant women …( text omitted ). Job aids are visual support materials that provide the right kind of information using graphics and words in a simple and yet effective manner. When nurses are not highly trained or have many work details to attend to, these job aids can serve as a content reminder for the nurses and can be used for educating their patients (Jennings, Yebadokpo, Affo, & Agbogbe, 2010) ( text omitted ). Importantly, additional evidence is needed to confirm how job aids can further improve the quality of ANC counseling by health workers in maternal care …( text omitted )” 28
• “ This has led us to hypothesize that the quality of ANC counseling would be better if supported by job aids. Consequently, a better quality of ANC counseling is expected to produce higher levels of awareness concerning the danger signs of pregnancy and a more favorable impression of the caring behavior of nurses .” 28
• “This study aimed to examine the differences in the responses of pregnant women to a job aid-supported intervention during ANC visit in terms of 1) their understanding of the danger signs of pregnancy and 2) their impression of the caring behaviors of nurses to pregnant women in rural Tanzania.” 28
• EXAMPLE 2. Background, hypotheses, and aims are provided
• “We conducted a two-arm randomized controlled trial (RCT) to evaluate and compare changes in salivary cortisol and oxytocin levels of first-time pregnant women between experimental and control groups. The women in the experimental group touched and held an infant for 30 min (experimental intervention protocol), whereas those in the control group watched a DVD movie of an infant (control intervention protocol). The primary outcome was salivary cortisol level and the secondary outcome was salivary oxytocin level.” 29
• “ We hypothesize that at 30 min after touching and holding an infant, the salivary cortisol level will significantly decrease and the salivary oxytocin level will increase in the experimental group compared with the control group .” 29
• EXAMPLE 3. Background, aim, and hypothesis are provided
• “In countries where the maternal mortality ratio remains high, antenatal education to increase Birth Preparedness and Complication Readiness (BPCR) is considered one of the top priorities [1]. BPCR includes birth plans during the antenatal period, such as the birthplace, birth attendant, transportation, health facility for complications, expenses, and birth materials, as well as family coordination to achieve such birth plans. In Tanzania, although increasing, only about half of all pregnant women attend an antenatal clinic more than four times [4]. Moreover, the information provided during antenatal care (ANC) is insufficient. In the resource-poor settings, antenatal group education is a potential approach because of the limited time for individual counseling at antenatal clinics.” 30
• “This study aimed to evaluate an antenatal group education program among pregnant women and their families with respect to birth-preparedness and maternal and infant outcomes in rural villages of Tanzania.” 30
• “ The study hypothesis was if Tanzanian pregnant women and their families received a family-oriented antenatal group education, they would (1) have a higher level of BPCR, (2) attend antenatal clinic four or more times, (3) give birth in a health facility, (4) have less complications of women at birth, and (5) have less complications and deaths of infants than those who did not receive the education .” 30

Research questions and hypotheses are crucial components to any type of research, whether quantitative or qualitative. These questions should be developed at the very beginning of the study. Excellent research questions lead to superior hypotheses, which, like a compass, set the direction of research, and can often determine the successful conduct of the study. Many research studies have floundered because the development of research questions and subsequent hypotheses was not given the thought and meticulous attention needed. The development of research questions and hypotheses is an iterative process based on extensive knowledge of the literature and insightful grasp of the knowledge gap. Focused, concise, and specific research questions provide a strong foundation for constructing hypotheses which serve as formal predictions about the research outcomes. Research questions and hypotheses are crucial elements of research that should not be overlooked. They should be carefully thought of and constructed when planning research. This avoids unethical studies and poor outcomes by defining well-founded objectives that determine the design, course, and outcome of the study.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Conceptualization: Barroga E, Matanguihan GJ.
• Methodology: Barroga E, Matanguihan GJ.
• Writing - original draft: Barroga E, Matanguihan GJ.
• Writing - review & editing: Barroga E, Matanguihan GJ.

A QUANTITATIVE ASSESSMENT OF VISUAL FUNCTION FOR YOUNG AND MEDICALLY COMPLEX CHILDREN WITH CEREBRAL VISUAL IMPAIRMENT: DEVELOPMENT AND INTER-RATER RELIABILITY

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Background Cerebral Visual Impairment (CVI) is the most common cause of low vision in children. Standardized, quantifiable measures of visual function are needed.

Objective This study developed and evaluated a new method for quantifying visual function in young and medically complex children with CVI using remote videoconferencing.

Methods Children diagnosed with CVI who had been unable to complete clinic-based recognition acuity tests were recruited from a low-vision rehabilitation clinic(n=22)Video-based Visual Function Assessment (VFA) was implemented using videoconference technology. Three low-vision rehabilitation clinicians independently scored recordings of each child’s VFA. Interclass correlations for inter-rater reliability was analyzed using intraclass correlations (ICC). Correlations were estimated between the video-based VFA scores and both clinically obtained acuity measures and children’s cognitive age equivalence.

Results Inter-rater reliability was analyzed using intraclass correlations (ICC). Correlations were estimated between the VFA scores, clinically obtained acuity measures, and cognitive age equivalence. ICCs showed good agreement (ICC and 95% CI 0.835 (0.701-0.916)) on VFA scores across raters and agreement was comparable to that from previous, similar studies. VFA scores strongly correlated (r= -0.706, p=0.002) with clinically obtained acuity measures. VFA scores and the cognitive age equivalence were moderately correlated (r= 0.518, p=0.005), with notable variation in VFA scores for participants below a ten month cognitive age-equivalence. The variability in VFA scores among children with lowest cognitive age-equivalence may have been an artifact of the study’s scoring method, or may represent existent variability in visual function for children with the lowest cognitive age-equivalence.

Conclusions Our new VFA is a reliable, quantitative measure of visual function for young and medically complex children with CVI. Future study of the VFA intrarater reliability and validity is warranted.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

This work was supported by the EyeSight Foundation of Alabama, Alie B. Gorrie Low Vision Research Fund and Research to Prevent Blindness. Additional support came from the National Institutes of Health [UL1 TR003096 to R.O.] and Grant T32 HS013852.

Author Declarations

I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.

The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

IRB of the University of Alabama at Birmingham gave ethical approval for this work

I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals.

I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).

I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable.

Data Availability

All data produced in the present study are available upon reasonable request to the authors

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Exploring Quantitative Biological Major, Trace, and Ultratrace Elements Composition and Qualitative Primary-Secondary Metabolites in Lamiaceae Medicinal Plants from Turkey

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• Published: 14 May 2024

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• Enes Tekman 1 ,
• Tugay Asgarlı 2 ,
• Hafize Yuca 2 ,
• Alptuğ Atila 3 ,
• Ömer Çeçen 4 &
• Songül Karakaya 1  

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Medicinal plants comprise a spectrum of constituents, encompassing both organic and inorganic elements. Elemental composition of 27 species of medicinal plants of Lamiaceae (including 17 endemic) family grown in Turkey was carried out by ICP-MS. The following elements were determined in analysed samples: Na, Mg, Al, K, Ca, Sc, Cr, Mn, Fe, Co, Zn, As, Rb, Sr, Cs, Ba, La, Ce, Sm, U, Se. Quantitative analysis of specific primary and secondary metabolites was carried out. Na and K are major constituents in plants. The concentrations of Na range from 332,495.590 g/kg (in sample 10SA) to 279,690.674 g/kg (in sample 4SA), while those of K vary from 67,492.456 g/kg (in sample 15SA) to 3347.612 g/kg (in sample 1A). Some metals such as Al, Cr, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Cs, and Ba were also detected. Flavonoids, carbohydrates and tannins were present in all sample. Saponins were found in all samples except 1C and 2O. Coumarin were detected in samples 2N, 1 T, 1O, 1Z, 3SA, 1C, 4SA, 6SA, 8SA, 1 M, 11SA, 13SA, 2O, 14SA, 1H, and 16SI. Lipids were present in samples 6S, 9S, 1A, 10S, 1 M, 11SA, 12SA, 13SA, 14SA, and 16SI. Plants contain essential, rare earth, and trace elements at mg/kg concentrations, while major elements such as K and Na are present in high levels. Toxic element As (arsenic) was detected in all analyzed plants, but in most samples, its concentration was below the threshold set by World Health Organization.

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Introduction

Medicinal plants comprise a spectrum of constituents, encompassing both organic and inorganic elements. The presence of macronutrients and trace (micro-) elements in medicinal plants serves as a rich source, playing a pivotal role in preventing a myriad of diseases [ 1 ]. Herbal remedies derived from medicinal plants have been employed as therapeutic agents since ancient times. Various plant components, including leaves, flowers, stems, roots, seeds, and bark, are utilized either individually or in synergistic combinations for their medicinal properties. The efficacy of these plants lies in their bioactive phytochemical constituents, which elicit specific physiological responses in the human body. Phytochemicals can be broadly categorized into two groups based on their functions within the plant. Primary metabolites (PMs), such as sugars, amino acids, proteins, lipids, and chlorophyll, are essential for basic growth processes. In contrast, secondary metabolites (SMs), including alkaloids, essential oils, flavonoids, tannins, terpenoids, saponins, phenolic compounds, and cardiac glycosides, play a crucial role in the plant's defense mechanisms against herbivores and other inter-species threats. These SMs contribute to the intricate pharmacological profile of medicinal plants, making them valuable resources in traditional and modern medicine alike. SMs in plants serve not only as a diverse array of natural products but also play a crucial role in fortifying the plant's defense mechanisms against pathogenic assaults and environmental stressors. Possessing noteworthy biological activities, these plant SM are gaining prominence as key components in medicinal formulations and food additives, catering to therapeutic, aromatic, and culinary needs. Research into plant secondary metabolites has witnessed a steady rise over the past five decades [ 2 , 3 , 4 , 5 ].

In the realm of agricultural science, prevailing challenges affecting global crop production encompass issues related to nutrient management, the presence of heavy and toxic metals, and the quest for optimal plant productivity. Plants, enduring for numerous decades, exhibit a diverse composition of elements in varying proportions. The elements present in plants are broadly categorized as either macronutrients, including Ca, K, Mg, N, P, and S, or micronutrients such as B, Cu, Cl, Fe, Mn, Mo, Na, and Zn. These elements play a pivotal role in the developmental processes and growth of plants, underscoring their significance in the agricultural landscape [ 6 ]. Plants frequently assimilate nutrients in quantities surpassing their immediate requirements, necessitating the storage of these excess nutrients within plant tissues until they are needed. Despite the selective uptake of essential micro- and macronutrients and the implementation of sophisticated exclusion mechanisms, plants unavoidably absorb elements that possess toxicity [ 7 ]. Elements exist in various forms in nature, and their presence is indispensable for the body to execute diverse functions. Trace elements hold paramount importance in facilitating cellular functions at biological, chemical, and molecular levels. These elements play a pivotal role in orchestrating important biochemical reactions by serving as cofactors for numerous enzymes and acting as central components for stabilizing the structures of enzymes and proteins. Certain elements exert control over crucial biological processes by binding to molecules on the receptor sites of cell membranes or by altering the membrane structure to impede the entry of specific molecules into the cell. The functions of trace elements exhibit a dual nature; at normal levels, they are crucial for stabilizing cellular structures, yet in deficiency states, they may stimulate alternative pathways, leading to various diseases. Disruptions in the balance of trace elements can lead to the onset of pathological conditions and diseases [ 8 , 9 ].

The Lamiaceae , commonly known as the mint family, exhibits a broad distribution across various natural ecosystems, encompassing 236 genera. Characterized by square stems in cross-section, opposite leaves, and zygomorphic flowers with five united petals and sepals, these aromatic plants are cultivated for their straightforward propagation through methods such as stem cutting [ 10 ]. The Lamiaceae (Labiatae) family boasts numerous medicinal plants of considerable value. Within this family, there are an estimated 6900 to 7200 species. Among the most prolific genera are Salvia (with approximately 900 species), Scutellaria (360 species), Stachys (300 species), Plectranthus (300 species), Hyptis (280 species), Teucrium (250 species), Vitex (250 species), Thymus (220 species), and Nepeta (200 species) [ 11 ]. Lamiaceae stands as the third-largest family in terms of taxon number and the fourth-largest based on species count in Turkey. With 48 genera and a total of 782 taxa (603 species, 179 subspecies, and varieties), 346 of these taxa (comprising 271 species, 75 subspecies, and varieties) are endemic, constituting approximately 44% of the family's diversity in the country (data updated as of February 1, 2017). Additionally, there are 23 hybrid species, 19 of which exhibit endemism (82%). These findings underscore Turkey's significance as one of the primary centers of diversity for Lamiaceae in the Old World. The flora of Turkey holds significant importance for the Lamiaceae family [ 10 , 12 ]. Lamiaceae is celebrated for housing a variety of active secondary metabolites that hold substantial biological and economic significance. These compounds encompass volatile oils containing monoterpenes and sesquiterpenes, diterpenes, triterpenes, phenolic acids, flavonoids, and other substances, each contributing diverse properties [ 13 ].

The majority of species within this plant family are known for their aromatic qualities and contain essential oils, rendering them highly valuable in the fields of cosmetics, perfumery, agriculture, food, and medicine [ 14 ]. Throughout history, the species belonging to the Lamiaceae family have held a storied tradition of utilization for flavoring, food preservation, and medicinal applications, owing to their dual benefits of healing and preventative attributes. It is widely acknowledged that each species possesses a unique and intricate blend of bioactive compounds, where each component plays a role in its overall bioactivity. These plants are highly esteemed for their capability to produce an extensive array of secondary metabolites, showcasing potent antibacterial, antioxidant, anti-inflammatory, antimicrobial, antiviral, and anticancer activities [ 15 ].

The trace elements are presently recognized and classified by the World Health Organization (WHO) into three groups: essential elements, elements considered likely to be essential, and elements with potential toxicity. Man necessitates essential trace elements in quantities spanning from 50 µg to 18 mg per day, wherein they function as catalytic or structural components within larger molecules [ 16 ]. In our study, the major elements identified in Lamiaceae plants are sodium (Na) and potassium (K). Sodium and potassium play indispensable roles in human health, serving as crucial ions within the body and being intricately linked to numerous physiological and pathophysiological processes [ 17 ].

In this research, we undertook an evaluation of various elements in 27 (including 17 endemic) herb species belonging to the Lamiaceae family, gathered in Turkey, utilizing ICP-MS analysis. Moreover, a quantitative analysis of specific primary and secondary metabolites was carried out. A substantial number of the analyzed herbs are readily available and widely utilized voluntarily by the entire population of the country.

Material and Method

Plant materials.

The names of the plants used, the localities where they were collected, their herbarium numbers and endemism status are given in Table  1 . A map showcasing the plant collecting sites across Turkey is presented in Fig.  1 .

A map showcasing the plant collecting sites across Turkey

The extraction process utilized in the study involved a mobile maceration technique, wherein 10 g of the dried flowering above-ground parts of each plant were meticulously weighed and then transformed into powder form. Subsequently, this powdered plant material was subjected to a mobile maceration process with 150 ml methanol (after 8 h, it was filtered and 150 ml of methanol was added again and this process was continued for 3 days), which typically involves soaking the plant material in a solvent at room temperature for a specific duration. During the mobile maceration process, the powdered plant material was immersed in a suitable solvent. The choice of solvent can vary depending on the specific compounds being targeted for extraction and their solubility characteristics. Commonly used solvents for maceration with methanol. The duration of maceration typically ranged from 3 to 8 h at room temperature. This duration allows sufficient time for the solvent to penetrate the plant material and extract the desired compounds. After the designated maceration period, the filtrates obtained from each day's extraction were systematically combined. Subsequently, the combined filtrates underwent evaporation using a rotavapor. Rotavapor, short for rotary evaporator, is a common laboratory instrument used for gentle evaporation of solvents from a mixture under reduced pressure. This process helps concentrate the extracted compounds, leaving behind a more concentrated extract for further analysis. Overall, the mobile maceration process employed in the study involved soaking the powdered plant material in a solvent for a specific duration at room temperature to facilitate the extraction of bioactive compounds. This method allows for efficient extraction while preserving the integrity of the extracted compounds. At the conclusion of each day, the filtrates were systematically amalgamated and subjected to evaporation using a rotavapor.

Qualitative Analysis of Secondary and Primary Metabolites

Detection of alkaloids.

The methanolic extract of each species was weighed 0.5 g, 10 ml of 70% ethanol solution (containing 6% H 2 SO 4 ) was added, boiled for 1 min and was cooled. Some of the extract was separated into 2 tubes and Mayer and Dragendorff reagents were added. The samples were checked for the appearance of precipitates and further experiments were continued for the samples in which precipitates were observed. After this control, the ethanol extract was alkalinized with a 25% Na 2 CO 3 solution. Then, extracted with 15 ml of chloroform, the chloroformed layer was taken into a clean separating funnel and extracted with 15 ml of 10% acetic acid solution. The acetic acid phase was divided into three separate tubes and one tube was kept as a control, the second tube added Mayer's reagents and the third tube added Dragendorff's reagents. It was checked for the precipitate formation [ 18 , 19 , 20 ].

Detection of Flavonoids

The methanolic extract of each species was weighed 0.5 g and extracted with water. The test solution was extracted with ethyl acetate into a separatory funnel and the ethyl acetate phase was capsulated and dried in a water bath. The residue remaining in the capsule was dissolved with a mixture of HCl:MeOH:H 2 O (1:1:1) and transferred to a test tube. After adding Mg powder, the color was observed and the flavonoid was identified [ 18 , 19 , 20 ].

Detection of Cardioactive Heterosides

The methanolic extract of each species was weighed 0.5 g and prepared as a solution with 10 ml 70% ethanol. Add 1 ml of concentrated lead subacetate solution to the solution, mixed and filtered. The filtrate was extracted with 10 ml of chloroform, the chloroform layer was separated, taken into a capsule and evaporated. Add 3 ml of 3.5% glacial acetic acid solution of FeCl 3 to the capsule and carefully transfer to 2 ml of concentrated sulfuric acid in a test tube to form a layer. The colors seen on the surface of the separation and in the acetic acid layers were observed [ 18 , 19 , 20 ].

Detection of Saponosides

The methanolic extract of each species was taken 0.5 g and placed in a test tube with 10 ml of hot water. After cooling, the tube was shaken strongly for 10 s. If saponoside was present, a 1–10 cm high foam layer was observed which remained stable for at least 10 min. Then 1–2 drops of 2N HCl were added and the persistence of the foam layer was observed. The methanolic extract of 0.5 g of each species was dissolved in chloroform and filtered. Add an equal volume of concentrated H 2 SO 4 to the filtrate. The presence of fluorescence color in the chloroform layer and in the acidic layer was observed. The reaction is based on the coloration of the [ 18 , 19 , 20 ].

Detection of Anthocyanosides

The methanol extract of each species was extracted in 50% ethanol. The extract was filtered, the filtrate was separated into five portions and the following reactions were performed:

The addition of diluted H 2 SO 4 resulted in the formation of red color.

First NaOH solution was added, then diluted HCl was added and the colors were observed.

The precipitate was observed with a 10% lead acetate solution.

Added a volume of amyl alcohol, shaken and observed the coloration of the layers [ 18 , 19 , 20 ].

Detection of Tannins

The methanol extract of each species was taken in a spatula to make an aqueous extract. The extract was divided into 3 parts and the following reactions were applied:

2 ml of 1% 1% saline gelatin solution (saturated with NaCl) was added and the precipitate was observed.

Tannins give a color reaction with iron salts. This reaction was determined with 5% FeCl 3 . Olive green and blue-black color formation was observed.

Brominated water was added and the precipitate were observed [ 18 , 19 , 20 ].

Detection of Anthracene Heterosides

The methanol extract of each species was taken at 0.5 g and boiled with diluted H 2 SO 4 . After filtering while hot, the filtrate was cooled. The extract was extracted with ether to eliminate the ether layer and shaken with 10% ammonia solution. The color in the underlying ammonia layer was observed [ 18 , 19 , 20 ].

Detection of Coumarin

The methanol extract of each species was taken 0.5 g, extracted in 10 ml ethanol and filtered. The filtrate was concentrated to dryness in a capsule and 1N NaOH solution was added. It was transferred into a tube and checked for fluorescence color at UV 366 nm [ 18 , 19 , 20 ].

Detection of Starch

The methanol extract of each species was taken 0.5 g and aqueous extract was prepared. Add a few drops of 0.1 N Iodine solution. The formation of purple color was checked [ 18 , 19 , 20 ].

Detection of Lipid

The extract of each species was made with petroleum ether and 5 ml was taken in a glass tube. It was concentrated in a water bath and applied as a stain on a filter paper. The filter paper was allowed to dry in an oven at 100 °C for 5 min. The oily stain was observed on the paper [ 18 , 19 , 20 ].

Detection of Carbohydrates

The methanol extract of each species was weighed 1 g and 6 ml of water was added to make an aqueous extract. It was filtered and the filtrate was divided into 3 tubes. The following experiments were applied to the solutions in the tubes respectively:

2 ml of Fehling A and 2 ml of Fehling B solutions were added to an empty tube and mixed. Then the test solution was added to it. The tube was heated and red colored precipitate.

1–2 drops of 15% solution of 1-naphthol in ethanol was added to 1 ml of the test solution. To this mixture, 1 ml of concentrated H 2 SO 4 . Purple ring formation was observed between the two layers.

Add a few crystals of resorcin to 1 ml of test solution. To this 1 ml of concentrated HCl was added and kept in a water bath. The presence of red color was observed [ 18 , 19 , 20 ].

Detection of Cyanogenetic Heterosites

0.5 g of the methanol extract of each species was taken into a flask and some water was added. The filter paper was wetted first with picric acid and then with sodium carbonate solution. The wetted filter paper was dropped into the flask. The flask was heated and red color formation was observed on the yellow colored filter paper [ 18 , 19 , 20 ].

ICP-MS Analysis of 21 Elements

The determination of elemental concentrations in the solution was carried out using an Inductively Coupled Plasma-Mass Spectrometer (ICP-MS), specifically the Agilent 7800 series provided by Agilent Technologies in Japan. For sample introduction, a glass MicroMist nebulizer from U-series in Australia and a double-pass quartz spray chamber from the USA were employed. The plasma system included a quartz torch (2.5 mm) and nickel components, including a sample cone and skimmer cone for the x-lens. Before sample analysis, all quartz and nickel components underwent a meticulous cleaning process. Quartz and glass elements were soaked in a 5–10% HNO 3 solution overnight, thoroughly rinsed with distilled water, dried in an oven, and then installed on the device. Nickel components, such as sample and skimmer cone pieces, underwent ultrasonic baths in pure water, 5% HNO 3 solution, and distilled water successively for five minutes each. Following this, they were cleaned with a cotton ball, rinsed thoroughly with distilled water, and dried in the oven before installation on the device. Before analysis, the device underwent a 45-min helium gas purge. Activation was followed by adjusting parameters, including Plasma gas (15 L/min), auxiliary gas (1 L/min), carrier gas (1 L/min), makeup/dilution gas (1 L/min), and carrier gas pressure (1.45 kPa). The plasma gas, auxiliary gas, carrier gas, and makeup/dilution gas were Argon (Ar).Torch axis, resolution axis, EM, standard lenses tune, plasma correction, full spectrum, and performance report tests were carried out sequentially. Calibration of the device utilized a tuning solution (1 µg/L Ce, Co, Li, Mg, Tl, Y). The values obtained during tuning were scrutinized to identify any deviations in the device. Standard solutions, prepared from stock solutions, were then analyzed, and calibration curves were verified within the standard reference range (0–10, 25, 50, 100, 250, 500 g/kg, mg/kg, and µg/kg for Na, Mg, Al, K, Ca, Sc, Cr, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Cs, Ba, La, Ce, Sm, and U elements.

Qualitative Analysis of Secondary Metabolites

The qualitative analysis of primary and secondary metabolites for the samples is detailed in Table  2 . Flavonoids were identified in all samples, while tannins were present in every sample. Saponins were found in all samples except 1C and 2O. Coumarin was detected in various samples, including 2N, 1 T, 1O, 1Z, 3SA, 1C, 4SA, 6SA, 8SA, 1 M, 11SA, 13SA, 2O, 14SA, 1H, and 16SI. Cardioactive heterosides were absent in all samples. Starch was identified solely in samples 4SA and 10SA. Lipids were present in samples 6S, 9S, 1A, 10S, 1 M, 11SA, 12SA, 13SA, 14SA, and 16SI, while carbohydrates were observed in all samples. Notably, no alkaloids, anthocyanosides, anthracene heterosides, or cyanogenetic heterosides were detected among the samples. The qualitative analysis data of primary and secondary metabolites for the methanolic extracts were provided in Table  2 .

Elemental Analysis

The analysis of the elemental composition of methanol extracts revealed notable variations in element concentrations, as outlined in Table  3 . Using ICP-MS, the methanol extracts were investigated for the presence of 21 elements, including Na, Mg, Al, K, Ca, Sc, Cr, Mn, Fe, Co, Zn, As, Rb, Sr, Cs, Ba, La, Ce, Sm, U, and Se. The results indicated elevated levels of Na [ranging from 332,495.590 g/kg (in sample 10SA) to 279,690.674 g/kg (in sample 4SA) g/kg] and K [ranging from 67,492.456 g/kg (in sample 15SA) to 3347.612 g/kg (in sample 1A)]. Some heavy metals such as Al, Cr, Mn, Fe, Co, Zn, As, Se, Rb, Sr, Cs, Ba, La, Ce, Sm (except samples 1SI, 2SA-12SA), and U (including samples 15SA and 16 SI) were also detected.

A comprehensive review of literature from 2002 to 2018 revealed various classes of secondary metabolites in this family, including flavonoids, fatty derivatives, and sterols [ 13 ].

In a review article, a total of 217 articles were selected from the initial search focusing on Lamiaceae , specifically those recognizing Mexican genera and species. The bioactive constituents identified in these genera predominantly include terpenes (both volatile and non-volatile) and phenolic compounds, particularly flavonoids in the form of glycosides and aglycones [ 22 ].

A review provided an overview of Nepeta species, focusing on their phytochemical characteristics. Terpenoids and phenolic compounds were predominantly identified through the application of chromatographic and spectroscopic techniques [ 23 ]. In another review, major constituents as nepetalactones, iridoids and their glucosides, diterpenes, triterpenes, and flavonoids, as well as essential oil, have been identified within Nepeta species [ 24 ]. The phytochemical composition and trace elements of Nepeta suavis were examined in an analysis. The findings unveiled the existence of bioactive components, including flavonoids, alkaloids, phenols, saponins, and tannins. Furthermore, the herb proved to be a rich reservoir of essential minerals such as Na, K, Ca, Mg, Zn, Fe, and P [ 25 ]. The volatile oils stand out as the primary constituents of the Thymus genus. In addition to these, the genus is rich in flavonoids, phenylpropanoids, tannins, organic acids, terpenoids, and phytosterols [ 26 ]. The Origanum genus exhibits chemical variations. Oregano, recognized for its distinctive flavor, is primarily associated with several plant species known for producing essential oils rich in carvacrol. Additionally, the genus consists of a diverse range of compounds, including terpenes, phenols such as phenolic acids, and flavonoids [ 27 ]. Numerous chemical constituents have been elucidated within the Sideritis genus, encompassing terpenes, flavonoids, essential oils, iridoids, coumarins, lignans, and sterols. Diterpenes, flavonoids, and essential oils are consistently present in nearly every species, serving as the primary components responsible for the pharmacological activity [ 28 ].

In a study, the aerial parts of Sideritis and Origanum species were analyzed for mineral contents, flavonoids, total phenols, and anthocyanins. K was found to be high in both plant species. In Sideritis , K contents ranged from 10,184.91 mg/kg ( Sideritis libanotica subsp. linearis ) to 17,182.86 mg/kg ( S. hispida ), while in Origanum , they ranged from 10,265.40 mg/kg ( Origanum majorana ) to 21,293.79 mg/kg ( O. vulgare ). Crude protein contents in Sideritis varied between 1.55% ( S. libanotica subsp. linearis ) and 7.83% ( S. perfoliata ), whereas protein contents in Origanum species ranged from 1.99% ( O. leptocladum ) to 5.51% ( O. vulgare ). Flavonoid contents in Sideritis plants ranged from 246.34 ( S. libatotica subsp. linearis ) to 2013.33 ( S. hololeuca ), and in Origanum plants, they varied from 345.38 ( O. onites ) to 1730.47 ( O. majorana ). For Origanum , K contents ranged between 10,265.40 mg/kg ( O. majorana ) and 21,293.79 mg/kg ( O. vulgare ) [ 29 ].

The terpenoids and flavonoids constitute the primary secondary metabolite constituents of Salvia , with over 80% being terpenoids, particularly abietane and clerodane diterpenoids. Notably, sesquiterpenoids and triterpenoids are relatively scarce in Salvia species. Numerous studies highlight the presence of flavonoids, triterpenoids, and monoterpenes, particularly in the flowers and leaves, while diterpenoids are predominantly found in the roots. However, literature surveys indicate that certain American Salvia species also contain diterpenoids in aerial parts, and in some Salvia species, triterpenoids and flavones are present in the roots [ 30 ]

In a research, Salvia officinalis , S. sclarea , S. pratensis , and S. nemorosa originating from Hungary and Transylvania were investigated for their tannin and flavonoid content. Significant differences (p < 0.05) were observed in tannin content between Hungarian and Transylvanian S. officinalis and flavonoid content between Hungarian and Transylvanian S. sclarea . Chromium content was notably high in all examined species. The element concentrations also differed significantly in aqueous extracts, with distinct dissolution rates. Notably, Hungarian S. officinalis exhibited elevated concentrations of Al, Fe, Mn, and Ti, possibly linked to soil pollution. Zinc accumulation was highest in Transylvanian S. officinalis and S. pratensis , while Hungarian S. nemorosa demonstrated elevated Li content. Chromium content was notably high in Hungarian S. officinalis and S. sclarea . Dissolution rates of elements varied widely among sage teas, showcasing significant differences in element concentrations (Al, B, Ba, Cu, Fe, K, Mg, Mn, Na, P, S, Zn) and dissolution characteristics based on the sample and the element studied [ 31 ].

The genus Ziziphora has been extensively studied, and previous reports in the literature highlight its species as rich sources of valuable bioactive compounds, including sterols, fatty acids, caffeoyl derivatives, and flavonoids. Notably, among the different groups of natural compounds, flavonoids, flavones, and their derivatives exhibit the highest frequency in the separated and characterized bioactive compounds, contributing significantly to each profile [ 32 ].

In literature, studies have indicated that Cyclotrichium essential oils are abundant in phenolic and alcoholic compounds, along with monoterpenes. Phenolic compounds are widely believed to be abundant in most Cyclotrichium species. C. origanifolium is rich in flavonoid [ 33 ]. According to our knowledge, this is the first elemental analysis conducted on the Cyclotrichium genus.

A comprehensive review aimed to provide an in-depth summary of the genus Ajuga L. Currently, more than 280 chemical constituents have been isolated and characterized from Ajuga species, with neo-clerodane diterpenes and diterpenoids, phytoecdysteroids, flavonoids, and iridoids identified as the major bioactive compounds [ 34 ]. This study represents the inaugural investigation into the elemental analysis of Ajuga chamaepitys .

In a comprehensive literature review on the genus Marrubium , chemical characteristics were investigated. The biological effects of the plants are often attributed to the presence of diterpenes, sterols, phenylpropanoids, and flavonoids [ 35 ].

Hyssop ( Hyssopus sp. ) genus has been known with volatile oils. The genus also containe flavonoid in leaves, flowers, stalks, and roots during full flowering. Furthermore, hyssop seeds have fatty acid content in oil [ 36 ].

In a study conducted similarly to our research, the elemental composition of 45 medicinal plant species belonging to the Lamiaceae family in the Republic of Moldova was investigated. Various elements, including essential, rare earth, and trace elements (measured in μg/L), as well as major elements such as Ca, Cl, K, Mg, and Na (measured in mg/L), were determined in the analyzed samples. Despite the presence of the toxic element As in all plants, its concentration in the majority of samples was below the limit set [ 37 ].

The study conducted in Pakistan, aimed to investigate the phytochemical, and elemental aspects of five crucial medicinal plants from the Lamiaceae family, namely Mentha longifolia , M. piperita , M. spicata , Ocimum basilicum , and Rosmarinus officinalis , collected in Peshawar district. Quantitative analysis of macro- and microminerals identified 13 elements (C, N, O, Mg, K, P, S, Ca, Al, Si, Fe, Cl, and Na) present in varying amounts among species. Methanol extracts from leaves were analyzed for phytochemical constituents such as saponins, flavonoids, tannins, terpenoids, phlobatannins, steroids, and anthraquinones [ 38 ].

Another research aimed to analyze the elemental content of six medicinal plant species from the Lamiaceae family, predominantly found in the western part of Romania. The findings indicate that these medicinal plants are rich sources of nutrients, with high potassium (K) content, followed by calcium (Ca), magnesium (Mg), iron (Fe), and zinc (Zn) [ 39 ].

A study explored the mineral elements of extract from N. italica subsp. cadmea . Mineral elements (P, Mg, K, Fe, Cu) were found in the extract [ 40 ]. A paper focused on the analysis of trace elements in the Indian medicinal plant Nepeta hindostana . The plant was identified as a rich source of essential trace elements as Na, K, Mg, Zn, Fe, and Mn [ 41 ].

A study investigated the concentrations of eleven mineral and trace elements (Mg, Ca, K, Fe, Mn, Al, Zn, Cu, Cd, Ni, and Pb) in various Thymus species in Turkey, including the Thymus cilicicus . The elemental concentrations (mg/g) in T. cilicicus were found to be 0.40 ± 0.02 (Pb), 0.20 ± 0.10 (Ni), 0.04 ± 0.01 (Cd), 10.70 ± 2.30 (Cu), 40.80 ± 4.70 (Zn), 191.0 ± 8.0 (Al), 19.0 ± 2.0 (Mn), 189.0 ± 2.0 (Fe), 12,250.0 ± 672.0 (K), 14,417.0 ± 1909.0 (Ca), and 4127.0 ± 1200.0 (Mg). T. cilicicus stands out as particularly rich in essential elements, with the highest concentrations observed for Ca, K, and Mg [ 42 ].

In a study, elemental concentrations in Ziziphora medicinal plants were analyzed. Samples were collected from the Eynali mountain region in the north of Tabriz, Iran. The study revealed varying concentrations of Al, Ca, K, Mg, Mn, Na, V, Cl, and Ti elements in powdered Ziziphora plants. Notably, Ziziphora plants exhibited richness in essential elements such as Mg, K, and Ca, all of importance for human health. Importantly, the concentration of the potentially toxic element, Al, was determined to be below the levels permitted by the World Health Organization (WHO) [ 43 ].

In research, to rationalize its medicinal applications and establish a biogeochemical link, the mineral elements (Na, K, Ca, Mg, Zn, Mn, Cu, Fe, Cr) present in the leaves and roots of Ajuga bracteosa were investigated. The herb exhibited comparatively higher amounts of chromium (25 mg per 100 g in leaves and 20 mg in roots), which may be associated with its traditional use as a remedy for diabetes. Additionally, the herb contains significantly higher levels of potassium (139 mg per 100 g in leaves and 159 mg in roots) compared to sodium (21 mg per 100 g in leaves and 29 mg in roots), suggesting a potential correlation with its use in managing hypertension [ 44 ].

According to our knowledge, this is the first elemental analysis conducted on the Marrubium globosum. In another study, instrumental neutron activation analysis identified twenty-two chemical elements in Marrubium vulgare . The study revealed that K was the dominant chemical element in the plant, constituting 4.40% of the mass. Ca and Fe mass fractions were also relatively high. Importantly, potential toxic elements in this Lamiaceae plant were found to be within the safety limits recommended by WHO/FAO [ 45 ].

A study considered four harvest heights (15, 25, 35, and 45 cm from the tip of the Hyssopus officinalis ) along with the residual stalks. Dependent variables included the accumulated content of elements (N, K, P, Ca, Mg, Cu, Zn, and Pb) at different heights. Results revealed that moving from the upper shoots towards the ground led to an increase in Mg, Ca, Cu, Zn, and Pb content by 22.67%, 43.74%, 12.87%, 39.02%, and 85.04%, respectively. Conversely, a downward trend was observed for N (50.16%) and K (6.41%) content, while an upward trend was noted for P (29.06%) content. In residual stalks, moving from upper shoots to the ground resulted in decreased Mg, Ca, Cu, Zn, and Pb contents by 1.01%, 21.03%, 9.11%, 17.02%, and 51.06%, respectively. However, N and P contents increased by 60.59% and 3.15%, respectively, with a 34.74% increase in P content [ 46 ].

The comprehensive analysis of primary and secondary metabolites, alongside elemental composition evaluation of methanol extracts, offers profound insights into the characteristics and potential functionalities of the samples. The ubiquitous presence of flavonoids and tannins across all samples hints at the antioxidant capabilities and potentially broader health-promoting effects of the plants under study. Flavonoids, renowned for their antioxidant and anti-inflammatory properties, stand as focal points in numerous health studies. Conversely, tannins, recognized for antimicrobial and antiviral attributes, contribute to the overall pharmacological profile of these plants. The absence of cardioactive heterosides suggests a lack of pronounced effects on cardiac function, which could be advantageous or constraining depending on the intended application of these botanicals. The detection of coumarin in varied samples sparks interest due to its diverse pharmacological spectrum, encompassing anticoagulant, anti-inflammatory, and antimicrobial facets. This discovery implies that the plants may harbor therapeutic potential extending beyond mere antioxidant capacities. The presence of lipids in several samples and the occurrence of starch in select ones accentuate the nutritional diversity inherent in these botanicals. Lipids, vital constituents of cellular membranes, and starch, a pivotal energy source, contribute to the overall nutritional value of these plants. Regarding elemental composition, the elevated levels of sodium and potassium throughout the samples hint at their potential mineral richness. However, the identification of heavy metals like aluminum, chromium, and arsenic raises concerns regarding potential toxicity, particularly in samples exhibiting higher concentrations of these elements. The provided text offers a comprehensive overview of studies focusing on the Lamiaceae family, exploring the elemental composition and phytochemical characteristics of various plant species within this family. These studies shed light on the diverse array of bioactive compounds present in Lamiaceae plants, ranging from essential minerals to secondary metabolites like flavonoids, terpenoids, and phenolic compounds. One notable aspect highlighted in the text is the prevalence of essential elements such as K, Ca, Mg, and Fe across different Lamiaceae species. These elements play crucial roles in various physiological processes, including enzymatic reactions, cellular signalling, and structural support. The abundance of these minerals underscores the potential nutritional significance of Lamiaceae plants in human diets. Additionally, the presence of bioactive compounds like flavonoids, terpenoids, and phenolic compounds underscores the therapeutic potential of Lamiaceae plants. These compounds have been associated with various pharmacological activities, including antioxidant, anti-inflammatory, and antimicrobial properties. The diverse chemical profile of Lamiaceae plants suggests their potential utility in traditional medicine and as sources of novel therapeutic agents. Furthermore, the studies discussed in the text also address the elemental variability among different Lamiaceae species, as well as the influence of environmental factors on elemental composition. Understanding these variations is crucial for assessing the nutritional quality and potential health benefits of Lamiaceae plants, as well as for elucidating their ecological roles and adaptation strategies. Overall, the findings presented in the text underscore the importance of interdisciplinary approaches in studying plant chemistry and ecology. Integrating elemental analysis with phytochemical profiling provides valuable insights into the nutritional, medicinal, and ecological significance of Lamiaceae plants, ultimately contributing to our understanding of their roles in human health and natural ecosystems.

In summary, these findings underscore the intricate nature of plant metabolites and emphasize the necessity for exhaustive analysis in deciphering their potential health implications. Further investigations, encompassing pharmacological and toxicological inquiries, are imperative for a comprehensive understanding of the therapeutic prospects and safety profiles associated with these botanicals.

In conclusion, the comprehensive analysis of 27 medicinal plant species from the Lamiaceae family in Turkey revealed a diverse spectrum of constituents, encompassing both organic and inorganic elements. The elemental composition, including 18 endemic species, was determined using ICP/MS, highlighting the presence of essential, rare earth, and trace elements at mg/kg concentrations. Major elements such as Na and K were found in elevated levels, demonstrating the richness of these plants in important nutrients. Despite the widespread detection of the toxic element As (arsenic), the concentrations in most samples remained below the World Health Organization's established threshold, assuring the safety of these medicinal plants. The inclusion of specific primary and secondary metabolites, such as flavonoids, tannins, saponins, coumarin, starch, lipids, and carbohydrates, further underscored the diverse chemical composition of these plants. Overall, this study provides valuable insights into the elemental and chemical profiles of medicinal plants from the Lamiaceae family, contributing to our understanding of their potential therapeutic properties and nutritional benefits. The presence of essential elements in trace amounts, coupled with the relatively low levels of toxic elements, reinforces the potential of these plants as valuable resources for traditional and modern medicine.

Data Availability

Data will be made available on request.

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Acknowledgements

Enes TEKMAN extends appreciation for the scholarship granted by the Turkish Scientific and Technical Research Council (TÜBİTAK) and acknowledges the assistance received during their postgraduate program.

Open access funding provided by the Scientific and Technological Research Council of Türkiye (TÜBİTAK).

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Enes Tekman & Songül Karakaya

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Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey

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Department of Plant and Animal Production, Vocational School of Technical Sciences, Karamanoğlu Mehmetbey University, 70200, Karaman, Turkey

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Contributions

Conceptualization: E.T., T.A., H.Y., A.A., Ö.Ç., S.K. Data curation: E.T., T.A., H.Y., A.A., Ö.Ç., S.K. Formal analysis and investigation: E.T., T.A., H.Y., A.A., S.K. Software: H.Y., S.K. Supervision: S.K. Resourches: Ö.Ç. Writing original draft: E.T., H.Y., S.K. Review and editing: H.Y., S.K. The manuscript has been checked and approved by all authors.

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Correspondence to Songül Karakaya .

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Tekman, E., Asgarlı, T., Yuca, H. et al. Exploring Quantitative Biological Major, Trace, and Ultratrace Elements Composition and Qualitative Primary-Secondary Metabolites in Lamiaceae Medicinal Plants from Turkey. Biol Trace Elem Res (2024). https://doi.org/10.1007/s12011-024-04219-z

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Received : 15 March 2024

Accepted : 02 May 2024

Published : 14 May 2024

DOI : https://doi.org/10.1007/s12011-024-04219-z

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