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Null Hypothesis: Definition, Rejecting & Examples

By Jim Frost 6 Comments

What is a Null Hypothesis?

The null hypothesis in statistics states that there is no difference between groups or no relationship between variables. It is one of two mutually exclusive hypotheses about a population in a hypothesis test.

• Null Hypothesis H 0 : No effect exists in the population.
• Alternative Hypothesis H A : The effect exists in the population.

In every study or experiment, researchers assess an effect or relationship. This effect can be the effectiveness of a new drug, building material, or other intervention that has benefits. There is a benefit or connection that the researchers hope to identify. Unfortunately, no effect may exist. In statistics, we call this lack of an effect the null hypothesis. Researchers assume that this notion of no effect is correct until they have enough evidence to suggest otherwise, similar to how a trial presumes innocence.

In this context, the analysts don’t necessarily believe the null hypothesis is correct. In fact, they typically want to reject it because that leads to more exciting finds about an effect or relationship. The new vaccine works!

You can think of it as the default theory that requires sufficiently strong evidence to reject. Like a prosecutor, researchers must collect sufficient evidence to overturn the presumption of no effect. Investigators must work hard to set up a study and a data collection system to obtain evidence that can reject the null hypothesis.

Related post : What is an Effect in Statistics?

Null Hypothesis Examples

Null hypotheses start as research questions that the investigator rephrases as a statement indicating there is no effect or relationship.

After reading these examples, you might think they’re a bit boring and pointless. However, the key is to remember that the null hypothesis defines the condition that the researchers need to discredit before suggesting an effect exists.

Let’s see how you reject the null hypothesis and get to those more exciting findings!

When to Reject the Null Hypothesis

So, you want to reject the null hypothesis, but how and when can you do that? To start, you’ll need to perform a statistical test on your data. The following is an overview of performing a study that uses a hypothesis test.

The first step is to devise a research question and the appropriate null hypothesis. After that, the investigators need to formulate an experimental design and data collection procedures that will allow them to gather data that can answer the research question. Then they collect the data. For more information about designing a scientific study that uses statistics, read my post 5 Steps for Conducting Studies with Statistics .

After data collection is complete, statistics and hypothesis testing enter the picture. Hypothesis testing takes your sample data and evaluates how consistent they are with the null hypothesis. The p-value is a crucial part of the statistical results because it quantifies how strongly the sample data contradict the null hypothesis.

When the sample data provide sufficient evidence, you can reject the null hypothesis. In a hypothesis test, this process involves comparing the p-value to your significance level .

Rejecting the Null Hypothesis

Reject the null hypothesis when the p-value is less than or equal to your significance level. Your sample data favor the alternative hypothesis, which suggests that the effect exists in the population. For a mnemonic device, remember—when the p-value is low, the null must go!

When you can reject the null hypothesis, your results are statistically significant. Learn more about Statistical Significance: Definition & Meaning .

Failing to Reject the Null Hypothesis

Conversely, when the p-value is greater than your significance level, you fail to reject the null hypothesis. The sample data provides insufficient data to conclude that the effect exists in the population. When the p-value is high, the null must fly!

Note that failing to reject the null is not the same as proving it. For more information about the difference, read my post about Failing to Reject the Null .

That’s a very general look at the process. But I hope you can see how the path to more exciting findings depends on being able to rule out the less exciting null hypothesis that states there’s nothing to see here!

Let’s move on to learning how to write the null hypothesis for different types of effects, relationships, and tests.

Related posts : How Hypothesis Tests Work and Interpreting P-values

How to Write a Null Hypothesis

The null hypothesis varies by the type of statistic and hypothesis test. Remember that inferential statistics use samples to draw conclusions about populations. Consequently, when you write a null hypothesis, it must make a claim about the relevant population parameter . Further, that claim usually indicates that the effect does not exist in the population. Below are typical examples of writing a null hypothesis for various parameters and hypothesis tests.

Related posts : Descriptive vs. Inferential Statistics and Populations, Parameters, and Samples in Inferential Statistics

Group Means

T-tests and ANOVA assess the differences between group means. For these tests, the null hypothesis states that there is no difference between group means in the population. In other words, the experimental conditions that define the groups do not affect the mean outcome. Mu (µ) is the population parameter for the mean, and you’ll need to include it in the statement for this type of study.

For example, an experiment compares the mean bone density changes for a new osteoporosis medication. The control group does not receive the medicine, while the treatment group does. The null states that the mean bone density changes for the control and treatment groups are equal.

• Null Hypothesis H 0 : Group means are equal in the population: µ 1 = µ 2 , or µ 1 – µ 2 = 0
• Alternative Hypothesis H A : Group means are not equal in the population: µ 1 ≠ µ 2 , or µ 1 – µ 2 ≠ 0.

Group Proportions

Proportions tests assess the differences between group proportions. For these tests, the null hypothesis states that there is no difference between group proportions. Again, the experimental conditions did not affect the proportion of events in the groups. P is the population proportion parameter that you’ll need to include.

For example, a vaccine experiment compares the infection rate in the treatment group to the control group. The treatment group receives the vaccine, while the control group does not. The null states that the infection rates for the control and treatment groups are equal.

• Null Hypothesis H 0 : Group proportions are equal in the population: p 1 = p 2 .
• Alternative Hypothesis H A : Group proportions are not equal in the population: p 1 ≠ p 2 .

Correlation and Regression Coefficients

Some studies assess the relationship between two continuous variables rather than differences between groups.

In these studies, analysts often use either correlation or regression analysis . For these tests, the null states that there is no relationship between the variables. Specifically, it says that the correlation or regression coefficient is zero. As one variable increases, there is no tendency for the other variable to increase or decrease. Rho (ρ) is the population correlation parameter and beta (β) is the regression coefficient parameter.

For example, a study assesses the relationship between screen time and test performance. The null states that there is no correlation between this pair of variables. As screen time increases, test performance does not tend to increase or decrease.

• Null Hypothesis H 0 : The correlation in the population is zero: ρ = 0.
• Alternative Hypothesis H A : The correlation in the population is not zero: ρ ≠ 0.

For all these cases, the analysts define the hypotheses before the study. After collecting the data, they perform a hypothesis test to determine whether they can reject the null hypothesis.

The preceding examples are all for two-tailed hypothesis tests. To learn about one-tailed tests and how to write a null hypothesis for them, read my post One-Tailed vs. Two-Tailed Tests .

Related post : Understanding Correlation

Neyman, J; Pearson, E. S. (January 1, 1933).  On the Problem of the most Efficient Tests of Statistical Hypotheses .  Philosophical Transactions of the Royal Society A .  231  (694–706): 289–337.

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January 11, 2024 at 2:57 pm

Thanks for the reply.

January 10, 2024 at 1:23 pm

Hi Jim, In your comment you state that equivalence test null and alternate hypotheses are reversed. For hypothesis tests of data fits to a probability distribution, the null hypothesis is that the probability distribution fits the data. Is this correct?

January 10, 2024 at 2:15 pm

Those two separate things, equivalence testing and normality tests. But, yes, you’re correct for both.

Hypotheses are switched for equivalence testing. You need to “work” (i.e., collect a large sample of good quality data) to be able to reject the null that the groups are different to be able to conclude they’re the same.

With typical hypothesis tests, if you have low quality data and a low sample size, you’ll fail to reject the null that they’re the same, concluding they’re equivalent. But that’s more a statement about the low quality and small sample size than anything to do with the groups being equal.

So, equivalence testing make you work to obtain a finding that the groups are the same (at least within some amount you define as a trivial difference).

For normality testing, and other distribution tests, the null states that the data follow the distribution (normal or whatever). If you reject the null, you have sufficient evidence to conclude that your sample data don’t follow the probability distribution. That’s a rare case where you hope to fail to reject the null. And it suffers from the problem I describe above where you might fail to reject the null simply because you have a small sample size. In that case, you’d conclude the data follow the probability distribution but it’s more that you don’t have enough data for the test to register the deviation. In this scenario, if you had a larger sample size, you’d reject the null and conclude it doesn’t follow that distribution.

I don’t know of any equivalence testing type approach for distribution fit tests where you’d need to work to show the data follow a distribution, although I haven’t looked for one either!

February 20, 2022 at 9:26 pm

Is a null hypothesis regularly (always) stated in the negative? “there is no” or “does not”

February 23, 2022 at 9:21 pm

Typically, the null hypothesis includes an equal sign. The null hypothesis states that the population parameter equals a particular value. That value is usually one that represents no effect. In the case of a one-sided hypothesis test, the null still contains an equal sign but it’s “greater than or equal to” or “less than or equal to.” If you wanted to translate the null hypothesis from its native mathematical expression, you could use the expression “there is no effect.” But the mathematical form more specifically states what it’s testing.

It’s the alternative hypothesis that typically contains does not equal.

There are some exceptions. For example, in an equivalence test where the researchers want to show that two things are equal, the null hypothesis states that they’re not equal.

In short, the null hypothesis states the condition that the researchers hope to reject. They need to work hard to set up an experiment and data collection that’ll gather enough evidence to be able to reject the null condition.

February 15, 2022 at 9:32 am

Dear sir I always read your notes on Research methods.. Kindly tell is there any available Book on all these..wonderfull Urgent

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• Null and Alternative Hypotheses | Definitions & Examples

Null and Alternative Hypotheses | Definitions & Examples

Published on 5 October 2022 by Shaun Turney . Revised on 6 December 2022.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis (H 0 ): There’s no effect in the population .
• Alternative hypothesis (H A ): There’s an effect in the population.

The effect is usually the effect of the independent variable on the dependent variable .

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, differences between null and alternative hypotheses, how to write null and alternative hypotheses, frequently asked questions about null and alternative hypotheses.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”, the null hypothesis (H 0 ) answers “No, there’s no effect in the population.” On the other hand, the alternative hypothesis (H A ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample.

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept. Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect”, “no difference”, or “no relationship”. When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis (H A ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect”, “a difference”, or “a relationship”. When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes > or <). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question
• They both make claims about the population
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis (H 0 ): Independent variable does not affect dependent variable .
• Alternative hypothesis (H A ): Independent variable affects dependent variable .

Test-specific

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Writing Null Hypotheses in Research and Statistics

Last Updated: January 17, 2024 Fact Checked

This article was co-authored by Joseph Quinones and by wikiHow staff writer, Jennifer Mueller, JD . Joseph Quinones is a High School Physics Teacher working at South Bronx Community Charter High School. Joseph specializes in astronomy and astrophysics and is interested in science education and science outreach, currently practicing ways to make physics accessible to more students with the goal of bringing more students of color into the STEM fields. He has experience working on Astrophysics research projects at the Museum of Natural History (AMNH). Joseph recieved his Bachelor's degree in Physics from Lehman College and his Masters in Physics Education from City College of New York (CCNY). He is also a member of a network called New York City Men Teach. There are 7 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 28,834 times.

Are you working on a research project and struggling with how to write a null hypothesis? Well, you've come to the right place! Start by recognizing that the basic definition of "null" is "none" or "zero"—that's your biggest clue as to what a null hypothesis should say. Keep reading to learn everything you need to know about the null hypothesis, including how it relates to your research question and your alternative hypothesis as well as how to use it in different types of studies.

Things You Should Know

• Write a research null hypothesis as a statement that the studied variables have no relationship to each other, or that there's no difference between 2 groups.

• Adjust the format of your null hypothesis to match the statistical method you used to test it, such as using "mean" if you're comparing the mean between 2 groups.

What is a null hypothesis?

• Research hypothesis: States in plain language that there's no relationship between the 2 variables or there's no difference between the 2 groups being studied.
• Statistical hypothesis: States the predicted outcome of statistical analysis through a mathematical equation related to the statistical method you're using.

Examples of Null Hypotheses

Null Hypothesis vs. Alternative Hypothesis

• For example, your alternative hypothesis could state a positive correlation between 2 variables while your null hypothesis states there's no relationship. If there's a negative correlation, then both hypotheses are false.

• You need additional data or evidence to show that your alternative hypothesis is correct—proving the null hypothesis false is just the first step.
• In smaller studies, sometimes it's enough to show that there's some relationship and your hypothesis could be correct—you can leave the additional proof as an open question for other researchers to tackle.

How do I test a null hypothesis?

• Group means: Compare the mean of the variable in your sample with the mean of the variable in the general population. [6] X Research source
• Group proportions: Compare the proportion of the variable in your sample with the proportion of the variable in the general population. [7] X Research source
• Correlation: Correlation analysis looks at the relationship between 2 variables—specifically, whether they tend to happen together. [8] X Research source
• Regression: Regression analysis reveals the correlation between 2 variables while also controlling for the effect of other, interrelated variables. [9] X Research source

Templates for Null Hypotheses

• Research null hypothesis: There is no difference in the mean [dependent variable] between [group 1] and [group 2].

• Research null hypothesis: The proportion of [dependent variable] in [group 1] and [group 2] is the same.

• Research null hypothesis: There is no correlation between [independent variable] and [dependent variable] in the population.

• Research null hypothesis: There is no relationship between [independent variable] and [dependent variable] in the population.

Expert Q&A

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Expert Interview

Thanks for reading our article! If you’d like to learn more about physics, check out our in-depth interview with Joseph Quinones .

• ↑ https://online.stat.psu.edu/stat100/lesson/10/10.1
• ↑ https://online.stat.psu.edu/stat501/lesson/2/2.12
• ↑ https://support.minitab.com/en-us/minitab/21/help-and-how-to/statistics/basic-statistics/supporting-topics/basics/null-and-alternative-hypotheses/
• ↑ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5635437/
• ↑ https://online.stat.psu.edu/statprogram/reviews/statistical-concepts/hypothesis-testing
• ↑ https://education.arcus.chop.edu/null-hypothesis-testing/
• ↑ https://sphweb.bumc.bu.edu/otlt/mph-modules/bs/bs704_hypothesistest-means-proportions/bs704_hypothesistest-means-proportions_print.html

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9.1 Null and Alternative Hypotheses

The actual test begins by considering two hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 , the — null hypothesis: a statement of no difference between sample means or proportions or no difference between a sample mean or proportion and a population mean or proportion. In other words, the difference equals 0.

H a —, the alternative hypothesis: a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make a decision. There are two options for a decision. They are reject H 0 if the sample information favors the alternative hypothesis or do not reject H 0 or decline to reject H 0 if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

Example 9.1

H 0 : No more than 30 percent of the registered voters in Santa Clara County voted in the primary election. p ≤ 30 H a : More than 30 percent of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25 percent. State the null and alternative hypotheses.

Example 9.2

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are the following: H 0 : μ = 2.0 H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 66
• H a : μ __ 66

Example 9.3

We want to test if college students take fewer than five years to graduate from college, on the average. The null and alternative hypotheses are the following: H 0 : μ ≥ 5 H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : μ __ 45
• H a : μ __ 45

Example 9.4

An article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third of the students pass. The same article stated that 6.6 percent of U.S. students take advanced placement exams and 4.4 percent pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6 percent. State the null and alternative hypotheses. H 0 : p ≤ 0.066 H a : p > 0.066

On a state driver’s test, about 40 percent pass the test on the first try. We want to test if more than 40 percent pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses.

• H 0 : p __ 0.40
• H a : p __ 0.40

Collaborative Exercise

Bring to class a newspaper, some news magazines, and some internet articles. In groups, find articles from which your group can write null and alternative hypotheses. Discuss your hypotheses with the rest of the class.

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Null Hypothesis Definition and Examples, How to State

What is the null hypothesis, how to state the null hypothesis, null hypothesis overview.

Why is it Called the “Null”?

The word “null” in this context means that it’s a commonly accepted fact that researchers work to nullify . It doesn’t mean that the statement is null (i.e. amounts to nothing) itself! (Perhaps the term should be called the “nullifiable hypothesis” as that might cause less confusion).

Why Do I need to Test it? Why not just prove an alternate one?

The short answer is, as a scientist, you are required to ; It’s part of the scientific process. Science uses a battery of processes to prove or disprove theories, making sure than any new hypothesis has no flaws. Including both a null and an alternate hypothesis is one safeguard to ensure your research isn’t flawed. Not including the null hypothesis in your research is considered very bad practice by the scientific community. If you set out to prove an alternate hypothesis without considering it, you are likely setting yourself up for failure. At a minimum, your experiment will likely not be taken seriously.

• Null hypothesis : H 0 : The world is flat.
• Alternate hypothesis: The world is round.

Several scientists, including Copernicus , set out to disprove the null hypothesis. This eventually led to the rejection of the null and the acceptance of the alternate. Most people accepted it — the ones that didn’t created the Flat Earth Society !. What would have happened if Copernicus had not disproved the it and merely proved the alternate? No one would have listened to him. In order to change people’s thinking, he first had to prove that their thinking was wrong .

How to State the Null Hypothesis from a Word Problem

You’ll be asked to convert a word problem into a hypothesis statement in statistics that will include a null hypothesis and an alternate hypothesis . Breaking your problem into a few small steps makes these problems much easier to handle.

Step 2: Convert the hypothesis to math . Remember that the average is sometimes written as μ.

H 1 : μ > 8.2

Broken down into (somewhat) English, that’s H 1 (The hypothesis): μ (the average) > (is greater than) 8.2

Step 3: State what will happen if the hypothesis doesn’t come true. If the recovery time isn’t greater than 8.2 weeks, there are only two possibilities, that the recovery time is equal to 8.2 weeks or less than 8.2 weeks.

H 0 : μ ≤ 8.2

Broken down again into English, that’s H 0 (The null hypothesis): μ (the average) ≤ (is less than or equal to) 8.2

How to State the Null Hypothesis: Part Two

But what if the researcher doesn’t have any idea what will happen.

Example Problem: A researcher is studying the effects of radical exercise program on knee surgery patients. There is a good chance the therapy will improve recovery time, but there’s also the possibility it will make it worse. Average recovery times for knee surgery patients is 8.2 weeks. 

Step 1: State what will happen if the experiment doesn’t make any difference. That’s the null hypothesis–that nothing will happen. In this experiment, if nothing happens, then the recovery time will stay at 8.2 weeks.

H 0 : μ = 8.2

Broken down into English, that’s H 0 (The null hypothesis): μ (the average) = (is equal to) 8.2

Step 2: Figure out the alternate hypothesis . The alternate hypothesis is the opposite of the null hypothesis. In other words, what happens if our experiment makes a difference?

H 1 : μ ≠ 8.2

In English again, that’s H 1 (The  alternate hypothesis): μ (the average) ≠ (is not equal to) 8.2

That’s How to State the Null Hypothesis!

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Gonick, L. (1993). The Cartoon Guide to Statistics . HarperPerennial. Kotz, S.; et al., eds. (2006), Encyclopedia of Statistical Sciences , Wiley.

15 Null Hypothesis Examples

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A null hypothesis is a general assertion or default position that there is no relationship or effect between two measured phenomena.

It’s a critical part of statistics, data analysis, and the scientific method . This concept forms the basis of testing statistical significance and allows researchers to be objective in their conclusions.

A null hypothesis helps to eliminate biases and ensures that the observed results are not due to chance. The rejection or failure to reject the null hypothesis helps in guiding the course of research.

Null Hypothesis Definition

The null hypothesis, often denoted as H 0 , is the hypothesis in a statistical test which proposes no statistical significance exists in a set of observed data.

It hypothesizes that any kind of difference or importance you see in a data set is due to chance.

Null hypotheses are typically proposed to be negated or disproved by statistical tests, paving way for the acceptance of an alternate hypothesis.

Importantly, a null hypothesis cannot be proven true; it can only be supported or rejected with confidence.

Should evidence – via statistical analysis – contradict the null hypothesis, it is rejected in favor of an alternative hypothesis. In essence, the null hypothesis is a tool to challenge and disprove that there is no effect or relationship between variables.

Video Explanation

I like to show this video to my students which outlines a null hypothesis really clearly and engagingly, using real life studies by research students! The into explains it really well:

“There’s an idea in science called the null hypothesis and it works like this: when you’re setting out to prove a theory, your default answer should be “it’s not going to work” and you have to convince the world otherwise through clear results”

Here’s the full video:

Null Hypothesis Examples

• Equality of Means: The null hypothesis posits that the average of group A does not differ from the average of group B. It suggests that any observed difference between the two group means is due to sampling or experimental error.
• No Correlation: The null hypothesis states there is no correlation between the variable X and variable Y in the population. It means that any correlation seen in sample data occurred by chance.
• Drug Effectiveness: The null hypothesis proposes that a new drug does not reduce the number of days to recover from a disease compared to a standard drug. Any observed difference is merely by chance and not due to the new drug.
• Classroom Teaching Method: The null hypothesis states that a new teaching method does not result in improved test scores compared to the traditional teaching method. Any improvement in scores can be attributed to chance or other unrelated factors.
• Smoking and Life Expectancy: The null hypothesis asserts that the average life expectancy of smokers is the same as that of non-smokers. Any perceived difference in life expectancy is due to random variation or other factors.
• Brand Preference: The null hypothesis suggests that the proportion of consumers preferring Brand A is the same as those preferring Brand B. Any observed preference in the sample is due to random selection.
• Vaccination Efficacy: The null hypothesis states that the efficacy of Vaccine A does not differ from that of Vaccine B. Any differences observed in a sample are due to chance or other confounding factors.
• Diet and Weight Loss: The null hypothesis proposes that following a specific diet does not result in more weight loss than not following the diet. Any weight loss observed among dieters is considered random or influenced by other factors.
• Exercise and Heart Rate: The null hypothesis states that regular exercise does not lower resting heart rate compared to no exercise. Any lower heart rates observed in exercisers could be due to chance or other unrelated factors.
• Climate Change: The null hypothesis asserts that the average global temperature this decade is not higher than the previous decade. Any observed temperature increase can be attributed to random variation or unaccounted factors.
• Gender Wage Gap: The null hypothesis posits that men and women earn the same average wage for the same job. Any observed wage disparity is due to chance or non-gender related factors.
• Psychotherapy Effectiveness: The null hypothesis states that patients undergoing psychotherapy do not show more improvement than those not undergoing therapy. Any improvement in the
• Energy Drink Consumption and Sleep: The null hypothesis proposes that consuming energy drinks does not affect the quantity of sleep. Any observed differences in sleep duration among energy drink consumers is due to random variation or other factors.
• Organic Food and Health: The null hypothesis asserts that consuming organic food does not lead to better health outcomes compared to consuming non-organic food. Any health differences observed in consumers of organic food are considered random or attributed to other confounding factors.
• Online Learning Effectiveness: The null hypothesis states that students learning online do not perform differently on exams than students learning in traditional classrooms. Any difference in performance can be attributed to chance or unrelated factors.

Null Hypothesis vs Alternative Hypothesis

An alternative hypothesis is the direct contrast to the null hypothesis. It posits that there is a statistically significant relationship or effect between the variables being observed.

If the null hypothesis is rejected based on the test data, the alternative hypothesis is accepted.

Importantly, while the null hypothesis is typically a statement of ‘no effect’ or ‘no difference,’ the alternative hypothesis states that there is an effect or difference.

Comprehension Checkpoint: How does the null hypothesis help to ensure that research is objective and unbiased?

Applications of the Null Hypothesis in Research

The null hypothesis plays a critical role in numerous research settings, promoting objectivity and ensuring findings aren’t due to random chance.

• Clinical Trials: Null hypothesis is used extensively in medical and pharmaceutical research. For example, when testing a new drug’s effectiveness, the null hypothesis might state that the drug has no effect on the disease. If data contradicts this, the null hypothesis is rejected, suggesting the drug might be effective.
• Business and Economics: Businesses use null hypotheses to make informed decisions. For instance, a company might use a null hypothesis to test if a new marketing strategy improves sales. If data suggests a significant increase in sales, the null hypothesis is rejected, and the new strategy may be implemented.
• Psychological Research: Psychologists use null hypotheses to test theories about behavior. For instance, a null hypothesis might state there’s no link between stress and sleep quality. Rejecting this hypothesis based on collected data could help establish a correlation between the two variables.
• Environmental Science: Null hypotheses are used to understand environmental changes. For instance, researchers might form a null hypothesis stating there is no significant difference in air quality before and after a policy change. If this hypothesis is rejected, it indicates the policy may have impacted air quality.
• Education: Educators and researchers often use null hypotheses to improve teaching methods. For example, a null hypothesis might propose a new teaching technique doesn’t enhance student performance. If data contradicts this, the technique may be beneficial.

In all these areas, the null hypothesis helps minimize bias, enabling researchers to support their findings with statistically significant data. It forms the backbone of many scientific research methodologies , promoting a disciplined approach to uncovering new knowledge.

See More Hypothesis Examples Here

The null hypothesis is a cornerstone of statistical analysis and empirical research. It serves as a starting point for investigations, providing a baseline premise that the observed effects are due to chance. By understanding and applying the concept of the null hypothesis, researchers can test the validity of their assumptions, making their findings more robust and reliable. In essence, the null hypothesis ensures that the scientific exploration remains objective, systematic, and free from unintended bias.

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Research Hypothesis In Psychology: Types, & Examples

Saul McLeod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul McLeod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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• Knowledge Base

Methodology

• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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• Math Article

Null Hypothesis

In mathematics, Statistics deals with the study of research and surveys on the numerical data. For taking surveys, we have to define the hypothesis. Generally, there are two types of hypothesis. One is a null hypothesis, and another is an alternative hypothesis .

In probability and statistics, the null hypothesis is a comprehensive statement or default status that there is zero happening or nothing happening. For example, there is no connection among groups or no association between two measured events. It is generally assumed here that the hypothesis is true until any other proof has been brought into the light to deny the hypothesis. Let us learn more here with definition, symbol, principle, types and example, in this article.

Table of contents:

• Comparison with Alternative Hypothesis

Null Hypothesis Definition

The null hypothesis is a kind of hypothesis which explains the population parameter whose purpose is to test the validity of the given experimental data. This hypothesis is either rejected or not rejected based on the viability of the given population or sample . In other words, the null hypothesis is a hypothesis in which the sample observations results from the chance. It is said to be a statement in which the surveyors wants to examine the data. It is denoted by H 0 .

Null Hypothesis Symbol

In statistics, the null hypothesis is usually denoted by letter H with subscript ‘0’ (zero), such that H 0 . It is pronounced as H-null or H-zero or H-nought. At the same time, the alternative hypothesis expresses the observations determined by the non-random cause. It is represented by H 1 or H a .

Null Hypothesis Principle

The principle followed for null hypothesis testing is, collecting the data and determining the chances of a given set of data during the study on some random sample, assuming that the null hypothesis is true. In case if the given data does not face the expected null hypothesis, then the outcome will be quite weaker, and they conclude by saying that the given set of data does not provide strong evidence against the null hypothesis because of insufficient evidence. Finally, the researchers tend to reject that.

Null Hypothesis Formula

Here, the hypothesis test formulas are given below for reference.

The formula for the null hypothesis is:

H 0 :  p = p 0

The formula for the alternative hypothesis is:

H a = p >p 0 , < p 0 ≠ p 0

The formula for the test static is:

Remember that,  p 0  is the null hypothesis and p – hat is the sample proportion.

Also, read:

Types of Null Hypothesis

There are different types of hypothesis. They are:

Simple Hypothesis

It completely specifies the population distribution. In this method, the sampling distribution is the function of the sample size.

Composite Hypothesis

The composite hypothesis is one that does not completely specify the population distribution.

Exact Hypothesis

Exact hypothesis defines the exact value of the parameter. For example μ= 50

Inexact Hypothesis

This type of hypothesis does not define the exact value of the parameter. But it denotes a specific range or interval. For example 45< μ <60

Null Hypothesis Rejection

Sometimes the null hypothesis is rejected too. If this hypothesis is rejected means, that research could be invalid. Many researchers will neglect this hypothesis as it is merely opposite to the alternate hypothesis. It is a better practice to create a hypothesis and test it. The goal of researchers is not to reject the hypothesis. But it is evident that a perfect statistical model is always associated with the failure to reject the null hypothesis.

How do you Find the Null Hypothesis?

The null hypothesis says there is no correlation between the measured event (the dependent variable) and the independent variable. We don’t have to believe that the null hypothesis is true to test it. On the contrast, you will possibly assume that there is a connection between a set of variables ( dependent and independent).

When is Null Hypothesis Rejected?

The null hypothesis is rejected using the P-value approach. If the P-value is less than or equal to the α, there should be a rejection of the null hypothesis in favour of the alternate hypothesis. In case, if P-value is greater than α, the null hypothesis is not rejected.

Null Hypothesis and Alternative Hypothesis

Now, let us discuss the difference between the null hypothesis and the alternative hypothesis.

Null Hypothesis Examples

Here, some of the examples of the null hypothesis are given below. Go through the below ones to understand the concept of the null hypothesis in a better way.

If a medicine reduces the risk of cardiac stroke, then the null hypothesis should be “the medicine does not reduce the chance of cardiac stroke”. This testing can be performed by the administration of a drug to a certain group of people in a controlled way. If the survey shows that there is a significant change in the people, then the hypothesis is rejected.

Few more examples are:

1). Are there is 100% chance of getting affected by dengue?

Ans: There could be chances of getting affected by dengue but not 100%.

2). Do teenagers are using mobile phones more than grown-ups to access the internet?

Ans: Age has no limit on using mobile phones to access the internet.

3). Does having apple daily will not cause fever?

Ans: Having apple daily does not assure of not having fever, but increases the immunity to fight against such diseases.

4). Do the children more good in doing mathematical calculations than grown-ups?

Ans: Age has no effect on Mathematical skills.

In many common applications, the choice of the null hypothesis is not automated, but the testing and calculations may be automated. Also, the choice of the null hypothesis is completely based on previous experiences and inconsistent advice. The choice can be more complicated and based on the variety of applications and the diversity of the objectives. 

The main limitation for the choice of the null hypothesis is that the hypothesis suggested by the data is based on the reasoning which proves nothing. It means that if some hypothesis provides a summary of the data set, then there would be no value in the testing of the hypothesis on the particular set of data. 

Frequently Asked Questions on Null Hypothesis

What is meant by the null hypothesis.

In Statistics, a null hypothesis is a type of hypothesis which explains the population parameter whose purpose is to test the validity of the given experimental data.

What are the benefits of hypothesis testing?

Hypothesis testing is defined as a form of inferential statistics, which allows making conclusions from the entire population based on the sample representative.

When a null hypothesis is accepted and rejected?

The null hypothesis is either accepted or rejected in terms of the given data. If P-value is less than α, then the null hypothesis is rejected in favor of the alternative hypothesis, and if the P-value is greater than α, then the null hypothesis is accepted in favor of the alternative hypothesis.

Why is the null hypothesis important?

The importance of the null hypothesis is that it provides an approximate description of the phenomena of the given data. It allows the investigators to directly test the relational statement in a research study.

How to accept or reject the null hypothesis in the chi-square test?

If the result of the chi-square test is bigger than the critical value in the table, then the data does not fit the model, which represents the rejection of the null hypothesis.

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10.1 - setting the hypotheses: examples.

A significance test examines whether the null hypothesis provides a plausible explanation of the data. The null hypothesis itself does not involve the data. It is a statement about a parameter (a numerical characteristic of the population). These population values might be proportions or means or differences between means or proportions or correlations or odds ratios or any other numerical summary of the population. The alternative hypothesis is typically the research hypothesis of interest. Here are some examples.

Example 10.2: Hypotheses with One Sample of One Categorical Variable Section  

About 10% of the human population is left-handed. Suppose a researcher at Penn State speculates that students in the College of Arts and Architecture are more likely to be left-handed than people found in the general population. We only have one sample since we will be comparing a population proportion based on a sample value to a known population value.

• Research Question : Are artists more likely to be left-handed than people found in the general population?
• Response Variable : Classification of the student as either right-handed or left-handed

State Null and Alternative Hypotheses

• Null Hypothesis : Students in the College of Arts and Architecture are no more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Art and Architecture = 10% or p = .10).
• Alternative Hypothesis : Students in the College of Arts and Architecture are more likely to be left-handed than people in the general population (population percent of left-handed students in the College of Arts and Architecture > 10% or p > .10). This is a one-sided alternative hypothesis.

Example 10.3: Hypotheses with One Sample of One Measurement Variable Section  

A generic brand of the anti-histamine Diphenhydramine markets a capsule with a 50 milligram dose. The manufacturer is worried that the machine that fills the capsules has come out of calibration and is no longer creating capsules with the appropriate dosage.

• Research Question : Does the data suggest that the population mean dosage of this brand is different than 50 mg?
• Response Variable : dosage of the active ingredient found by a chemical assay.
• Null Hypothesis : On the average, the dosage sold under this brand is 50 mg (population mean dosage = 50 mg).
• Alternative Hypothesis : On the average, the dosage sold under this brand is not 50 mg (population mean dosage ≠ 50 mg). This is a two-sided alternative hypothesis.

Example 10.4: Hypotheses with Two Samples of One Categorical Variable Section  

Many people are starting to prefer vegetarian meals on a regular basis. Specifically, a researcher believes that females are more likely than males to eat vegetarian meals on a regular basis.

• Research Question : Does the data suggest that females are more likely than males to eat vegetarian meals on a regular basis?
• Response Variable : Classification of whether or not a person eats vegetarian meals on a regular basis
• Explanatory (Grouping) Variable: Sex
• Null Hypothesis : There is no sex effect regarding those who eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis = population percent of males who eat vegetarian meals on a regular basis or p females = p males ).
• Alternative Hypothesis : Females are more likely than males to eat vegetarian meals on a regular basis (population percent of females who eat vegetarian meals on a regular basis > population percent of males who eat vegetarian meals on a regular basis or p females > p males ). This is a one-sided alternative hypothesis.

Example 10.5: Hypotheses with Two Samples of One Measurement Variable Section  

Obesity is a major health problem today. Research is starting to show that people may be able to lose more weight on a low carbohydrate diet than on a low fat diet.

• Research Question : Does the data suggest that, on the average, people are able to lose more weight on a low carbohydrate diet than on a low fat diet?
• Response Variable : Weight loss (pounds)
• Explanatory (Grouping) Variable : Type of diet
• Null Hypothesis : There is no difference in the mean amount of weight loss when comparing a low carbohydrate diet with a low fat diet (population mean weight loss on a low carbohydrate diet = population mean weight loss on a low fat diet).
• Alternative Hypothesis : The mean weight loss should be greater for those on a low carbohydrate diet when compared with those on a low fat diet (population mean weight loss on a low carbohydrate diet > population mean weight loss on a low fat diet). This is a one-sided alternative hypothesis.

Example 10.6: Hypotheses about the relationship between Two Categorical Variables Section  

• Research Question : Do the odds of having a stroke increase if you inhale second hand smoke ? A case-control study of non-smoking stroke patients and controls of the same age and occupation are asked if someone in their household smokes.
• Variables : There are two different categorical variables (Stroke patient vs control and whether the subject lives in the same household as a smoker). Living with a smoker (or not) is the natural explanatory variable and having a stroke (or not) is the natural response variable in this situation.
• Null Hypothesis : There is no relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is = 1).
• Alternative Hypothesis : There is a relationship between whether or not a person has a stroke and whether or not a person lives with a smoker (odds ratio between stroke and second-hand smoke situation is > 1). This is a one-tailed alternative.

This research question might also be addressed like example 11.4 by making the hypotheses about comparing the proportion of stroke patients that live with smokers to the proportion of controls that live with smokers.

Example 10.7: Hypotheses about the relationship between Two Measurement Variables Section  

• Research Question : A financial analyst believes there might be a positive association between the change in a stock's price and the amount of the stock purchased by non-management employees the previous day (stock trading by management being under "insider-trading" regulatory restrictions).
• Variables : Daily price change information (the response variable) and previous day stock purchases by non-management employees (explanatory variable). These are two different measurement variables.
• Null Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) = 0.
• Alternative Hypothesis : The correlation between the daily stock price change (\$) and the daily stock purchases by non-management employees (\$) > 0. This is a one-sided alternative hypothesis.

Example 10.8: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples Section  

• Research Question : Is there a linear relationship between the amount of the bill (\$) at a restaurant and the tip (\$) that was left. Is the strength of this association different for family restaurants than for fine dining restaurants?
• Variables : There are two different measurement variables. The size of the tip would depend on the size of the bill so the amount of the bill would be the explanatory variable and the size of the tip would be the response variable.
• Null Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the same at family restaurants as it is at fine dining restaurants.
• Alternative Hypothesis : The correlation between the amount of the bill (\$) at a restaurant and the tip (\$) that was left is the difference at family restaurants then it is at fine dining restaurants. This is a two-sided alternative hypothesis.

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Chapter 13: Inferential Statistics

Understanding Null Hypothesis Testing

Learning Objectives

• Explain the purpose of null hypothesis testing, including the role of sampling error.
• Describe the basic logic of null hypothesis testing.
• Describe the role of relationship strength and sample size in determining statistical significance and make reasonable judgments about statistical significance based on these two factors.

The Purpose of Null Hypothesis Testing

As we have seen, psychological research typically involves measuring one or more variables for a sample and computing descriptive statistics for that sample. In general, however, the researcher’s goal is not to draw conclusions about that sample but to draw conclusions about the population that the sample was selected from. Thus researchers must use sample statistics to draw conclusions about the corresponding values in the population. These corresponding values in the population are called  parameters . Imagine, for example, that a researcher measures the number of depressive symptoms exhibited by each of 50 clinically depressed adults and computes the mean number of symptoms. The researcher probably wants to use this sample statistic (the mean number of symptoms for the sample) to draw conclusions about the corresponding population parameter (the mean number of symptoms for clinically depressed adults).

Unfortunately, sample statistics are not perfect estimates of their corresponding population parameters. This is because there is a certain amount of random variability in any statistic from sample to sample. The mean number of depressive symptoms might be 8.73 in one sample of clinically depressed adults, 6.45 in a second sample, and 9.44 in a third—even though these samples are selected randomly from the same population. Similarly, the correlation (Pearson’s  r ) between two variables might be +.24 in one sample, −.04 in a second sample, and +.15 in a third—again, even though these samples are selected randomly from the same population. This random variability in a statistic from sample to sample is called  sampling error . (Note that the term error  here refers to random variability and does not imply that anyone has made a mistake. No one “commits a sampling error.”)

One implication of this is that when there is a statistical relationship in a sample, it is not always clear that there is a statistical relationship in the population. A small difference between two group means in a sample might indicate that there is a small difference between the two group means in the population. But it could also be that there is no difference between the means in the population and that the difference in the sample is just a matter of sampling error. Similarly, a Pearson’s  r  value of −.29 in a sample might mean that there is a negative relationship in the population. But it could also be that there is no relationship in the population and that the relationship in the sample is just a matter of sampling error.

In fact, any statistical relationship in a sample can be interpreted in two ways:

• There is a relationship in the population, and the relationship in the sample reflects this.
• There is no relationship in the population, and the relationship in the sample reflects only sampling error.

The purpose of null hypothesis testing is simply to help researchers decide between these two interpretations.

The Logic of Null Hypothesis Testing

Null hypothesis testing  is a formal approach to deciding between two interpretations of a statistical relationship in a sample. One interpretation is called the   null hypothesis  (often symbolized  H 0  and read as “H-naught”). This is the idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error. Informally, the null hypothesis is that the sample relationship “occurred by chance.” The other interpretation is called the  alternative hypothesis  (often symbolized as  H 1 ). This is the idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

Again, every statistical relationship in a sample can be interpreted in either of these two ways: It might have occurred by chance, or it might reflect a relationship in the population. So researchers need a way to decide between them. Although there are many specific null hypothesis testing techniques, they are all based on the same general logic. The steps are as follows:

• Assume for the moment that the null hypothesis is true. There is no relationship between the variables in the population.
• Determine how likely the sample relationship would be if the null hypothesis were true.
• If the sample relationship would be extremely unlikely, then reject the null hypothesis  in favour of the alternative hypothesis. If it would not be extremely unlikely, then  retain the null hypothesis .

Following this logic, we can begin to understand why Mehl and his colleagues concluded that there is no difference in talkativeness between women and men in the population. In essence, they asked the following question: “If there were no difference in the population, how likely is it that we would find a small difference of  d  = 0.06 in our sample?” Their answer to this question was that this sample relationship would be fairly likely if the null hypothesis were true. Therefore, they retained the null hypothesis—concluding that there is no evidence of a sex difference in the population. We can also see why Kanner and his colleagues concluded that there is a correlation between hassles and symptoms in the population. They asked, “If the null hypothesis were true, how likely is it that we would find a strong correlation of +.60 in our sample?” Their answer to this question was that this sample relationship would be fairly unlikely if the null hypothesis were true. Therefore, they rejected the null hypothesis in favour of the alternative hypothesis—concluding that there is a positive correlation between these variables in the population.

A crucial step in null hypothesis testing is finding the likelihood of the sample result if the null hypothesis were true. This probability is called the  p value . A low  p  value means that the sample result would be unlikely if the null hypothesis were true and leads to the rejection of the null hypothesis. A high  p  value means that the sample result would be likely if the null hypothesis were true and leads to the retention of the null hypothesis. But how low must the  p  value be before the sample result is considered unlikely enough to reject the null hypothesis? In null hypothesis testing, this criterion is called  α (alpha)  and is almost always set to .05. If there is less than a 5% chance of a result as extreme as the sample result if the null hypothesis were true, then the null hypothesis is rejected. When this happens, the result is said to be  statistically significant . If there is greater than a 5% chance of a result as extreme as the sample result when the null hypothesis is true, then the null hypothesis is retained. This does not necessarily mean that the researcher accepts the null hypothesis as true—only that there is not currently enough evidence to conclude that it is true. Researchers often use the expression “fail to reject the null hypothesis” rather than “retain the null hypothesis,” but they never use the expression “accept the null hypothesis.”

The Misunderstood  p  Value

The  p  value is one of the most misunderstood quantities in psychological research (Cohen, 1994) [1] . Even professional researchers misinterpret it, and it is not unusual for such misinterpretations to appear in statistics textbooks!

The most common misinterpretation is that the  p  value is the probability that the null hypothesis is true—that the sample result occurred by chance. For example, a misguided researcher might say that because the  p  value is .02, there is only a 2% chance that the result is due to chance and a 98% chance that it reflects a real relationship in the population. But this is incorrect . The  p  value is really the probability of a result at least as extreme as the sample result  if  the null hypothesis  were  true. So a  p  value of .02 means that if the null hypothesis were true, a sample result this extreme would occur only 2% of the time.

You can avoid this misunderstanding by remembering that the  p  value is not the probability that any particular  hypothesis  is true or false. Instead, it is the probability of obtaining the  sample result  if the null hypothesis were true.

Role of Sample Size and Relationship Strength

Recall that null hypothesis testing involves answering the question, “If the null hypothesis were true, what is the probability of a sample result as extreme as this one?” In other words, “What is the  p  value?” It can be helpful to see that the answer to this question depends on just two considerations: the strength of the relationship and the size of the sample. Specifically, the stronger the sample relationship and the larger the sample, the less likely the result would be if the null hypothesis were true. That is, the lower the  p  value. This should make sense. Imagine a study in which a sample of 500 women is compared with a sample of 500 men in terms of some psychological characteristic, and Cohen’s  d  is a strong 0.50. If there were really no sex difference in the population, then a result this strong based on such a large sample should seem highly unlikely. Now imagine a similar study in which a sample of three women is compared with a sample of three men, and Cohen’s  d  is a weak 0.10. If there were no sex difference in the population, then a relationship this weak based on such a small sample should seem likely. And this is precisely why the null hypothesis would be rejected in the first example and retained in the second.

Of course, sometimes the result can be weak and the sample large, or the result can be strong and the sample small. In these cases, the two considerations trade off against each other so that a weak result can be statistically significant if the sample is large enough and a strong relationship can be statistically significant even if the sample is small. Table 13.1 shows roughly how relationship strength and sample size combine to determine whether a sample result is statistically significant. The columns of the table represent the three levels of relationship strength: weak, medium, and strong. The rows represent four sample sizes that can be considered small, medium, large, and extra large in the context of psychological research. Thus each cell in the table represents a combination of relationship strength and sample size. If a cell contains the word  Yes , then this combination would be statistically significant for both Cohen’s  d  and Pearson’s  r . If it contains the word  No , then it would not be statistically significant for either. There is one cell where the decision for  d  and  r  would be different and another where it might be different depending on some additional considerations, which are discussed in Section 13.2 “Some Basic Null Hypothesis Tests”

Although Table 13.1 provides only a rough guideline, it shows very clearly that weak relationships based on medium or small samples are never statistically significant and that strong relationships based on medium or larger samples are always statistically significant. If you keep this lesson in mind, you will often know whether a result is statistically significant based on the descriptive statistics alone. It is extremely useful to be able to develop this kind of intuitive judgment. One reason is that it allows you to develop expectations about how your formal null hypothesis tests are going to come out, which in turn allows you to detect problems in your analyses. For example, if your sample relationship is strong and your sample is medium, then you would expect to reject the null hypothesis. If for some reason your formal null hypothesis test indicates otherwise, then you need to double-check your computations and interpretations. A second reason is that the ability to make this kind of intuitive judgment is an indication that you understand the basic logic of this approach in addition to being able to do the computations.

Statistical Significance Versus Practical Significance

Table 13.1 illustrates another extremely important point. A statistically significant result is not necessarily a strong one. Even a very weak result can be statistically significant if it is based on a large enough sample. This is closely related to Janet Shibley Hyde’s argument about sex differences (Hyde, 2007) [2] . The differences between women and men in mathematical problem solving and leadership ability are statistically significant. But the word  significant  can cause people to interpret these differences as strong and important—perhaps even important enough to influence the college courses they take or even who they vote for. As we have seen, however, these statistically significant differences are actually quite weak—perhaps even “trivial.”

This is why it is important to distinguish between the  statistical  significance of a result and the  practical  significance of that result.  Practical significance refers to the importance or usefulness of the result in some real-world context. Many sex differences are statistically significant—and may even be interesting for purely scientific reasons—but they are not practically significant. In clinical practice, this same concept is often referred to as “clinical significance.” For example, a study on a new treatment for social phobia might show that it produces a statistically significant positive effect. Yet this effect still might not be strong enough to justify the time, effort, and other costs of putting it into practice—especially if easier and cheaper treatments that work almost as well already exist. Although statistically significant, this result would be said to lack practical or clinical significance.

Key Takeaways

• Null hypothesis testing is a formal approach to deciding whether a statistical relationship in a sample reflects a real relationship in the population or is just due to chance.
• The logic of null hypothesis testing involves assuming that the null hypothesis is true, finding how likely the sample result would be if this assumption were correct, and then making a decision. If the sample result would be unlikely if the null hypothesis were true, then it is rejected in favour of the alternative hypothesis. If it would not be unlikely, then the null hypothesis is retained.
• The probability of obtaining the sample result if the null hypothesis were true (the  p  value) is based on two considerations: relationship strength and sample size. Reasonable judgments about whether a sample relationship is statistically significant can often be made by quickly considering these two factors.
• Statistical significance is not the same as relationship strength or importance. Even weak relationships can be statistically significant if the sample size is large enough. It is important to consider relationship strength and the practical significance of a result in addition to its statistical significance.
• Discussion: Imagine a study showing that people who eat more broccoli tend to be happier. Explain for someone who knows nothing about statistics why the researchers would conduct a null hypothesis test.
• The correlation between two variables is  r  = −.78 based on a sample size of 137.
• The mean score on a psychological characteristic for women is 25 ( SD  = 5) and the mean score for men is 24 ( SD  = 5). There were 12 women and 10 men in this study.
• In a memory experiment, the mean number of items recalled by the 40 participants in Condition A was 0.50 standard deviations greater than the mean number recalled by the 40 participants in Condition B.
• In another memory experiment, the mean scores for participants in Condition A and Condition B came out exactly the same!
• A student finds a correlation of  r  = .04 between the number of units the students in his research methods class are taking and the students’ level of stress.

Long Descriptions

“Null Hypothesis” long description: A comic depicting a man and a woman talking in the foreground. In the background is a child working at a desk. The man says to the woman, “I can’t believe schools are still teaching kids about the null hypothesis. I remember reading a big study that conclusively disproved it years ago.” [Return to “Null Hypothesis”]

“Conditional Risk” long description: A comic depicting two hikers beside a tree during a thunderstorm. A bolt of lightning goes “crack” in the dark sky as thunder booms. One of the hikers says, “Whoa! We should get inside!” The other hiker says, “It’s okay! Lightning only kills about 45 Americans a year, so the chances of dying are only one in 7,000,000. Let’s go on!” The comic’s caption says, “The annual death rate among people who know that statistic is one in six.” [Return to “Conditional Risk”]

Media Attributions

• Null Hypothesis by XKCD  CC BY-NC (Attribution NonCommercial)
• Conditional Risk by XKCD  CC BY-NC (Attribution NonCommercial)
• Cohen, J. (1994). The world is round: p < .05. American Psychologist, 49 , 997–1003. ↵
• Hyde, J. S. (2007). New directions in the study of gender similarities and differences. Current Directions in Psychological Science, 16 , 259–263. ↵

Values in a population that correspond to variables measured in a study.

The random variability in a statistic from sample to sample.

A formal approach to deciding between two interpretations of a statistical relationship in a sample.

The idea that there is no relationship in the population and that the relationship in the sample reflects only sampling error.

The idea that there is a relationship in the population and that the relationship in the sample reflects this relationship in the population.

When the relationship found in the sample would be extremely unlikely, the idea that the relationship occurred “by chance” is rejected.

When the relationship found in the sample is likely to have occurred by chance, the null hypothesis is not rejected.

The probability that, if the null hypothesis were true, the result found in the sample would occur.

How low the p value must be before the sample result is considered unlikely in null hypothesis testing.

When there is less than a 5% chance of a result as extreme as the sample result occurring and the null hypothesis is rejected.

Research Methods in Psychology - 2nd Canadian Edition Copyright © 2015 by Paul C. Price, Rajiv Jhangiani, & I-Chant A. Chiang is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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Module 9: Hypothesis Testing With One Sample

Null and alternative hypotheses, learning outcomes.

• Describe hypothesis testing in general and in practice

The actual test begins by considering two  hypotheses . They are called the null hypothesis and the alternative hypothesis . These hypotheses contain opposing viewpoints.

H 0 : The null hypothesis: It is a statement about the population that either is believed to be true or is used to put forth an argument unless it can be shown to be incorrect beyond a reasonable doubt.

H a : The alternative hypothesis : It is a claim about the population that is contradictory to H 0 and what we conclude when we reject H 0 .

Since the null and alternative hypotheses are contradictory, you must examine evidence to decide if you have enough evidence to reject the null hypothesis or not. The evidence is in the form of sample data.

After you have determined which hypothesis the sample supports, you make adecision. There are two options for a  decision . They are “reject H 0 ” if the sample information favors the alternative hypothesis or “do not reject H 0 ” or “decline to reject H 0 ” if the sample information is insufficient to reject the null hypothesis.

Mathematical Symbols Used in  H 0 and H a :

H 0 always has a symbol with an equal in it. H a never has a symbol with an equal in it. The choice of symbol depends on the wording of the hypothesis test. However, be aware that many researchers (including one of the co-authors in research work) use = in the null hypothesis, even with > or < as the symbol in the alternative hypothesis. This practice is acceptable because we only make the decision to reject or not reject the null hypothesis.

H 0 : No more than 30% of the registered voters in Santa Clara County voted in the primary election. p ≤ 30

H a : More than 30% of the registered voters in Santa Clara County voted in the primary election. p > 30

A medical trial is conducted to test whether or not a new medicine reduces cholesterol by 25%. State the null and alternative hypotheses.

H 0 : The drug reduces cholesterol by 25%. p = 0.25

H a : The drug does not reduce cholesterol by 25%. p ≠ 0.25

We want to test whether the mean GPA of students in American colleges is different from 2.0 (out of 4.0). The null and alternative hypotheses are:

H 0 : μ = 2.0

H a : μ ≠ 2.0

We want to test whether the mean height of eighth graders is 66 inches. State the null and alternative hypotheses. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : μ __ 66 H a : μ __ 66

• H 0 : μ = 66
• H a : μ ≠ 66

We want to test if college students take less than five years to graduate from college, on the average. The null and alternative hypotheses are:

H 0 : μ ≥ 5

H a : μ < 5

We want to test if it takes fewer than 45 minutes to teach a lesson plan. State the null and alternative hypotheses. Fill in the correct symbol ( =, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : μ __ 45 H a : μ __ 45

• H 0 : μ ≥ 45
• H a : μ < 45

In an issue of U.S. News and World Report , an article on school standards stated that about half of all students in France, Germany, and Israel take advanced placement exams and a third pass. The same article stated that 6.6% of U.S. students take advanced placement exams and 4.4% pass. Test if the percentage of U.S. students who take advanced placement exams is more than 6.6%. State the null and alternative hypotheses.

H 0 : p ≤ 0.066

H a : p > 0.066

On a state driver’s test, about 40% pass the test on the first try. We want to test if more than 40% pass on the first try. Fill in the correct symbol (=, ≠, ≥, <, ≤, >) for the null and alternative hypotheses. H 0 : p __ 0.40 H a : p __ 0.40

• H 0 : p = 0.40
• H a : p > 0.40

Concept Review

In a  hypothesis test , sample data is evaluated in order to arrive at a decision about some type of claim. If certain conditions about the sample are satisfied, then the claim can be evaluated for a population. In a hypothesis test, we: Evaluate the null hypothesis , typically denoted with H 0 . The null is not rejected unless the hypothesis test shows otherwise. The null statement must always contain some form of equality (=, ≤ or ≥) Always write the alternative hypothesis , typically denoted with H a or H 1 , using less than, greater than, or not equals symbols, i.e., (≠, >, or <). If we reject the null hypothesis, then we can assume there is enough evidence to support the alternative hypothesis. Never state that a claim is proven true or false. Keep in mind the underlying fact that hypothesis testing is based on probability laws; therefore, we can talk only in terms of non-absolute certainties.

Formula Review

H 0 and H a are contradictory.

• OpenStax, Statistics, Null and Alternative Hypotheses. Provided by : OpenStax. Located at : http://cnx.org/contents/[email protected]:58/Introductory_Statistics . License : CC BY: Attribution
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• Simple hypothesis testing | Probability and Statistics | Khan Academy. Authored by : Khan Academy. Located at : https://youtu.be/5D1gV37bKXY . License : All Rights Reserved . License Terms : Standard YouTube License

Null Hypothesis Definition and Examples

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In a scientific experiment, the null hypothesis is the proposition that there is no effect or no relationship between phenomena or populations. If the null hypothesis is true, any observed difference in phenomena or populations would be due to sampling error (random chance) or experimental error. The null hypothesis is useful because it can be tested and found to be false, which then implies that there is a relationship between the observed data. It may be easier to think of it as a nullifiable hypothesis or one that the researcher seeks to nullify. The null hypothesis is also known as the H 0, or no-difference hypothesis.

The alternate hypothesis, H A or H 1 , proposes that observations are influenced by a non-random factor. In an experiment, the alternate hypothesis suggests that the experimental or independent variable has an effect on the dependent variable .

How to State a Null Hypothesis

There are two ways to state a null hypothesis. One is to state it as a declarative sentence, and the other is to present it as a mathematical statement.

For example, say a researcher suspects that exercise is correlated to weight loss, assuming diet remains unchanged. The average length of time to achieve a certain amount of weight loss is six weeks when a person works out five times a week. The researcher wants to test whether weight loss takes longer to occur if the number of workouts is reduced to three times a week.

The first step to writing the null hypothesis is to find the (alternate) hypothesis. In a word problem like this, you're looking for what you expect to be the outcome of the experiment. In this case, the hypothesis is "I expect weight loss to take longer than six weeks."

This can be written mathematically as: H 1 : μ > 6

In this example, μ is the average.

Now, the null hypothesis is what you expect if this hypothesis does not happen. In this case, if weight loss isn't achieved in greater than six weeks, then it must occur at a time equal to or less than six weeks. This can be written mathematically as:

H 0 : μ ≤ 6

The other way to state the null hypothesis is to make no assumption about the outcome of the experiment. In this case, the null hypothesis is simply that the treatment or change will have no effect on the outcome of the experiment. For this example, it would be that reducing the number of workouts would not affect the time needed to achieve weight loss:

H 0 : μ = 6

Null Hypothesis Examples

"Hyperactivity is unrelated to eating sugar " is an example of a null hypothesis. If the hypothesis is tested and found to be false, using statistics, then a connection between hyperactivity and sugar ingestion may be indicated. A significance test is the most common statistical test used to establish confidence in a null hypothesis.

Another example of a null hypothesis is "Plant growth rate is unaffected by the presence of cadmium in the soil ." A researcher could test the hypothesis by measuring the growth rate of plants grown in a medium lacking cadmium, compared with the growth rate of plants grown in mediums containing different amounts of cadmium. Disproving the null hypothesis would set the groundwork for further research into the effects of different concentrations of the element in soil.

Why Test a Null Hypothesis?

You may be wondering why you would want to test a hypothesis just to find it false. Why not just test an alternate hypothesis and find it true? The short answer is that it is part of the scientific method. In science, propositions are not explicitly "proven." Rather, science uses math to determine the probability that a statement is true or false. It turns out it's much easier to disprove a hypothesis than to positively prove one. Also, while the null hypothesis may be simply stated, there's a good chance the alternate hypothesis is incorrect.

For example, if your null hypothesis is that plant growth is unaffected by duration of sunlight, you could state the alternate hypothesis in several different ways. Some of these statements might be incorrect. You could say plants are harmed by more than 12 hours of sunlight or that plants need at least three hours of sunlight, etc. There are clear exceptions to those alternate hypotheses, so if you test the wrong plants, you could reach the wrong conclusion. The null hypothesis is a general statement that can be used to develop an alternate hypothesis, which may or may not be correct.

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Once you have developed a clear and focused research question or set of research questions, you’ll be ready to conduct further research, a literature review, on the topic to help you make an educated guess about the answer to your question(s). This educated guess is called a hypothesis.

In research, there are two types of hypotheses: null and alternative. They work as a complementary pair, each stating that the other is wrong.

• Null Hypothesis (H 0 ) – This can be thought of as the implied hypothesis. “Null” meaning “nothing.”  This hypothesis states that there is no difference between groups or no relationship between variables. The null hypothesis is a presumption of status quo or no change.
• Alternative Hypothesis (H a ) – This is also known as the claim. This hypothesis should state what you expect the data to show, based on your research on the topic. This is your answer to your research question.

Null Hypothesis:   H 0 : There is no difference in the salary of factory workers based on gender. Alternative Hypothesis :  H a : Male factory workers have a higher salary than female factory workers.

Null Hypothesis :  H 0 : There is no relationship between height and shoe size. Alternative Hypothesis :  H a : There is a positive relationship between height and shoe size.

Null Hypothesis :  H 0 : Experience on the job has no impact on the quality of a brick mason’s work. Alternative Hypothesis :  H a : The quality of a brick mason’s work is influenced by on-the-job experience.

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Null Hypothesis

A hypothesis that states that there is no relationship between two population parameters

What is the Null Hypothesis?

The null hypothesis states that there is no relationship between two population parameters, i.e., an independent variable and a dependent variable . If the hypothesis shows a relationship between the two parameters, the outcome could be due to an experimental or sampling error. However, if the null hypothesis returns false, there is a relationship in the measured phenomenon.

The null hypothesis is useful because it can be tested to conclude whether or not there is a relationship between two measured phenomena. It can inform the user whether the results obtained are due to chance or manipulating a phenomenon. Testing a hypothesis sets the stage for rejecting or accepting a hypothesis within a certain confidence level.

Two main approaches to statistical inference in a null hypothesis can be used– significance testing by Ronald Fisher and hypothesis testing by Jerzy Neyman and Egon Pearson. Fisher’s significance testing approach states that a null hypothesis is rejected if the measured data is significantly unlikely to have occurred (the null hypothesis is false). Therefore, the null hypothesis is rejected and replaced with an alternative hypothesis.

If the observed outcome is consistent with the position held by the null hypothesis, the hypothesis is accepted. On the other hand, the hypothesis testing by Neyman and Pearson is compared to an alternative hypothesis to make a conclusion about the observed data. The two hypotheses are differentiated based on observed data.

• A null hypothesis refers to a hypothesis that states that there is no relationship between two population parameters.
• Researchers reject or disprove the null hypothesis to set the stage for further experimentation or research that explains the position of interest.
• The inverse of a null hypothesis is an alternative hypothesis, which states that there is statistical significance between two variables.

How the Null Hypothesis Works

A null hypothesis is a theory based on insufficient evidence that requires further testing to prove whether the observed data is true or false. For example, a null hypothesis statement can be “the rate of plant growth is not affected by sunlight.” It can be tested by measuring the growth of plants in the presence of sunlight and comparing this with the growth of plants in the absence of sunlight.

Rejecting the null hypothesis sets the stage for further experimentation to see a relationship between the two variables exists. Rejecting a null hypothesis does not necessarily mean that the experiment did not produce the required results, but it sets the stage for further experimentation.

To differentiate the null hypothesis from other forms of hypothesis, a null hypothesis is written as H 0 , while the alternate hypothesis is written as H A or H 1 . A significance test is used to establish confidence in a null hypothesis and determine whether the observed data is not due to chance or manipulation of data.

Researchers test the hypothesis by examining a random sample of the plants being grown with or without sunlight. If the outcome demonstrates a statistically significant change in the observed change, the null hypothesis is rejected.

Null Hypothesis Example

The annual return of ABC Limited bonds is assumed to be 7.5%. To test if the scenario is true or false, we take the null hypothesis to be “the mean annual return for ABC limited bond is not 7.5%.” To test the hypothesis, we first accept the null hypothesis.

Any information that is against the stated null hypothesis is taken to be the alternative hypothesis for the purpose of testing the hypotheses. In such a case, the alternative hypothesis is “the mean annual return of ABC Limited is 7.5%.”

We take samples of the annual returns of the bond for the last five years to calculate the sample mean for the previous five years. The result is then compared to the assumed annual return average of 7.5% to test the null hypothesis.

The average annual returns for the five-year period are 7.5%; the null hypothesis is rejected. Consequently, the alternative hypothesis is accepted.

What is an Alternative Hypothesis?

An alternative hypothesis is the inverse of a null hypothesis. An alternative hypothesis and a null hypothesis are mutually exclusive, which means that only one of the two hypotheses can be true.

A statistical significance exists between the two variables. If samples used to test the null hypothesis return false, it means that the alternate hypothesis is true, and there is statistical significance between the two variables.

Purpose of Hypothesis Testing

Hypothesis testing is a statistical process of testing an assumption regarding a phenomenon or population parameter. It is a critical part of the scientific method, which is a systematic approach to assessing theories through observations and determining the probability that a stated statement is true or false.

A good theory can make accurate predictions. For an analyst who makes predictions, hypothesis testing is a rigorous way of backing up his prediction with statistical analysis. It also helps determine sufficient statistical evidence that favors a certain hypothesis about the population parameter.

Additional Resources

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1.4 Hypothesis testing

8 min read • august 20, 2024

Hypothesis testing is a crucial statistical method in causal inference. It helps researchers make decisions about population parameters based on sample data, using null and alternative hypotheses to assess the significance of treatment effects and compare groups in experiments.

The process involves formulating hypotheses, selecting appropriate tests, and interpreting results. Key concepts include significance levels, p-values, and different types of errors. Researchers must consider limitations and practical significance when drawing conclusions about causal relationships.

Hypothesis testing overview

• Hypothesis testing is a statistical method used to make decisions about population parameters based on sample data
• It involves formulating a null hypothesis ( H 0 H_0 H 0 ​ ) and an alternative hypothesis ( H a H_a H a ​ ), and using probability to determine whether to reject or fail to reject the null hypothesis
• Hypothesis testing is a crucial tool in causal inference for assessing the significance of treatment effects and comparing groups in experiments

Null and alternative hypotheses

• The null hypothesis ( H 0 H_0 H 0 ​ ) states that there is no significant difference or relationship between variables, or that a parameter equals a specific value
• The alternative hypothesis ( H a H_a H a ​ ) is the opposite of the null hypothesis and represents the claim being tested, such as the existence of a significant difference or relationship
• H 0 H_0 H 0 ​ : The mean weight of a population is 150 lbs; H a H_a H a ​ : The mean weight is not 150 lbs
• H 0 H_0 H 0 ​ : There is no association between smoking and lung cancer; H a H_a H a ​ : There is an association between smoking and lung cancer

Significance level and p-values

• The significance level ( α \alpha α ) is the probability threshold for rejecting the null hypothesis, typically set at 0.05 or 0.01
• The p-value is the probability of observing a test statistic as extreme as or more extreme than the one calculated from the sample data, assuming the null hypothesis is true
• If the p-value is less than the significance level, the null hypothesis is rejected; otherwise, we fail to reject the null hypothesis
• A smaller p-value provides stronger evidence against the null hypothesis

One-tailed vs two-tailed tests

• A one-tailed test is used when the alternative hypothesis specifies a direction (greater than or less than), focusing on one tail of the distribution
• A two-tailed test is used when the alternative hypothesis does not specify a direction (not equal to), considering both tails of the distribution
• The choice between a one-tailed or two-tailed test depends on the research question and prior knowledge about the direction of the effect

Type I and Type II errors

• The probability of a Type I error is equal to the significance level ( α \alpha α )
• The probability of a Type II error is denoted by β \beta β
• The goal is to minimize both types of errors, but there is a trade-off between them

Power of a test

• The power of a test is the probability of rejecting the null hypothesis when it is actually false (i.e., correctly detecting a significant effect)
• Power is calculated as 1 − β 1 - \beta 1 − β , where β \beta β is the probability of a Type II error
• Factors that influence power include sample size, effect size , significance level, and test type
• Higher power reduces the risk of Type II errors and increases the likelihood of detecting true effects

Common hypothesis tests

• Various hypothesis tests are used depending on the type of data, distribution, and research question
• Some common tests include the z-test , t-test , chi-square test , and F-test
• Each test has specific assumptions and is suitable for different scenarios

Z-test for population mean

• The z-test is used to test hypotheses about a population mean when the population standard deviation is known and the sample size is large (n > 30) or the population is normally distributed
• The test statistic ( z z z ) is calculated as: z = x ˉ − μ 0 σ / n z = \frac{\bar{x} - \mu_0}{\sigma / \sqrt{n}} z = σ / n ​ x ˉ − μ 0 ​ ​ , where x ˉ \bar{x} x ˉ is the sample mean, μ 0 \mu_0 μ 0 ​ is the hypothesized population mean, σ \sigma σ is the population standard deviation, and n n n is the sample size
• The z-test assumes that the data are independently and identically distributed (i.i.d.) and that the population is normally distributed

T-test for sample mean

• The t-test is used to test hypotheses about a population mean when the population standard deviation is unknown and the sample size is small (n < 30), or when comparing the means of two independent or paired samples
• The test statistic ( t t t ) is calculated as: t = x ˉ − μ 0 s / n t = \frac{\bar{x} - \mu_0}{s / \sqrt{n}} t = s / n ​ x ˉ − μ 0 ​ ​ , where x ˉ \bar{x} x ˉ is the sample mean, μ 0 \mu_0 μ 0 ​ is the hypothesized population mean, s s s is the sample standard deviation, and n n n is the sample size
• The t-test assumes that the data are i.i.d. and that the population is normally distributed or that the sample size is large enough for the Central Limit Theorem to apply

Chi-square test for independence

• The chi-square test is used to test for the independence of two categorical variables
• It compares the observed frequencies in a contingency table to the expected frequencies under the null hypothesis of independence
• The test statistic ( χ 2 \chi^2 χ 2 ) is calculated as: χ 2 = ∑ ( O − E ) 2 E \chi^2 = \sum \frac{(O - E)^2}{E} χ 2 = ∑ E ( O − E ) 2 ​ , where O O O is the observed frequency and E E E is the expected frequency for each cell
• The chi-square test assumes that the expected frequencies are not too small (usually at least 5) and that the observations are independent

F-test for equality of variances

• The F-test is used to test for the equality of variances between two populations
• It compares the ratio of the sample variances to determine if they are significantly different
• The test statistic ( F F F ) is calculated as: F = s 1 2 s 2 2 F = \frac{s_1^2}{s_2^2} F = s 2 2 ​ s 1 2 ​ ​ , where s 1 2 s_1^2 s 1 2 ​ and s 2 2 s_2^2 s 2 2 ​ are the sample variances of the two populations
• The F-test assumes that the data are i.i.d., the populations are normally distributed, and the samples are independent

Steps in hypothesis testing

• Hypothesis testing follows a systematic procedure to ensure valid and reliable results
• The steps include formulating hypotheses, selecting an appropriate test, calculating the test statistic, determining the critical value, and making a decision based on the p-value

Formulating hypotheses

• Clearly state the null hypothesis ( H 0 H_0 H 0 ​ ) and the alternative hypothesis ( H a H_a H a ​ ) based on the research question and available information
• Ensure that the hypotheses are mutually exclusive and exhaustive
• Consider the implications of rejecting or failing to reject the null hypothesis

Selecting appropriate test

• Choose the appropriate hypothesis test based on the type of data (categorical or numerical), the distribution of the data (normal or non-normal), the sample size, and the research question
• Consider the assumptions of each test and whether they are met by the data
• Determine whether a one-tailed or two-tailed test is appropriate based on the alternative hypothesis

Calculating test statistic

• Calculate the test statistic using the appropriate formula for the selected hypothesis test
• Substitute the sample data and hypothesized values into the formula
• Double-check the calculations to ensure accuracy

Determining critical value

• Determine the critical value(s) based on the significance level ( α \alpha α ) and the type of test (one-tailed or two-tailed)
• Use statistical tables or software to find the critical value(s) for the specific test and degrees of freedom
• The critical value(s) represent the boundary between the rejection and non-rejection regions of the distribution

Making decision to reject or fail to reject

• Compare the calculated test statistic to the critical value(s) or calculate the p-value
• If the test statistic falls in the rejection region or the p-value is less than the significance level, reject the null hypothesis; otherwise, fail to reject the null hypothesis
• Interpret the decision in the context of the research question and the implications for the study

Interpreting results

• After conducting a hypothesis test, it is essential to interpret the results correctly and consider their implications
• Interpretation should include confidence intervals, effect sizes, practical significance, and limitations of the test

Confidence intervals

• A confidence interval is a range of values that is likely to contain the true population parameter with a certain level of confidence (usually 95%)
• Confidence intervals provide more information than a simple hypothesis test by indicating the precision and uncertainty of the estimate
• A narrow confidence interval suggests a more precise estimate, while a wide confidence interval indicates greater uncertainty

Effect size and practical significance

• The effect size measures the magnitude of the difference or relationship between variables, independent of the sample size
• Common effect size measures include Cohen's d, Pearson's r, and odds ratios
• Practical significance refers to the real-world importance or relevance of the effect, beyond statistical significance
• A statistically significant result may not be practically significant if the effect size is small or the consequences are minimal

Limitations of hypothesis testing

• It does not prove causality, only the existence of a significant relationship or difference
• It is sensitive to sample size, with large samples more likely to yield significant results even for small effects
• It can be affected by violations of assumptions, such as non-normality or lack of independence
• It does not account for multiple testing, which can inflate the Type I error rate
• Researchers should be cautious in drawing conclusions based solely on hypothesis tests and consider other factors, such as study design, data quality, and theoretical plausibility

Applications in causal inference

• Hypothesis testing is a key tool in causal inference for assessing the significance of treatment effects and comparing groups in experiments
• It helps researchers determine whether observed differences or relationships are likely due to chance or to a genuine causal effect

Testing for significant treatment effects

• In randomized controlled trials (RCTs) and other experimental designs, hypothesis testing is used to assess the significance of the difference between treatment and control groups
• A significant result suggests that the treatment has a causal effect on the outcome, while a non-significant result indicates that the observed difference could be due to chance
• Example: Testing whether a new drug significantly reduces blood pressure compared to a placebo

Comparing groups in experiments

• Hypothesis testing is also used to compare multiple groups in experiments, such as different treatment conditions or subpopulations
• Tests like ANOVA (analysis of variance) and post-hoc comparisons help determine which groups differ significantly from each other
• Example: Comparing the effectiveness of three different teaching methods on student performance

Assessing validity of causal claims

• Hypothesis testing can be used to assess the validity of causal claims made in observational studies or quasi-experiments
• By testing for significant associations between variables or differences between groups, researchers can evaluate the strength of the evidence for a causal relationship
• However, hypothesis testing alone cannot establish causality, as other factors like confounding and reverse causation must be considered

Hypothesis testing vs estimation approaches

• While hypothesis testing focuses on making decisions about the existence of effects or differences, estimation approaches aim to quantify the size and uncertainty of effects
• Estimation methods, such as confidence intervals and Bayesian analysis, provide more informative results than simple hypothesis tests
• In causal inference, a combination of hypothesis testing and estimation approaches is often used to assess the significance, magnitude, and precision of causal effects

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• Published: 23 August 2024

Ventral attention network connectivity is linked to cortical maturation and cognitive ability in childhood

• Hao-Ming Dong   ORCID: orcid.org/0000-0002-0176-2969 1 ,
• Xi-Han Zhang   ORCID: orcid.org/0000-0003-2630-0063 1 ,
• Loïc Labache   ORCID: orcid.org/0000-0002-5733-0743 1 ,
• Shaoshi Zhang 2 ,
• Leon Qi Rong Ooi   ORCID: orcid.org/0000-0002-3546-4580 2 ,
• B. T. Thomas Yeo   ORCID: orcid.org/0000-0002-0119-3276 2 , 3 , 4 ,
• Daniel S. Margulies   ORCID: orcid.org/0000-0002-8880-9204 5 ,
• Avram J. Holmes   ORCID: orcid.org/0000-0001-6583-803X 6   na1 &
• Xi-Nian Zuo   ORCID: orcid.org/0000-0001-9110-585X 7 , 8 , 9   na1  

Nature Neuroscience ( 2024 ) Cite this article

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• Cognitive neuroscience

The human brain experiences functional changes through childhood and adolescence, shifting from an organizational framework anchored within sensorimotor and visual regions into one that is balanced through interactions with later-maturing aspects of association cortex. Here, we link this profile of functional reorganization to the development of ventral attention network connectivity across independent datasets. We demonstrate that maturational changes in cortical organization link preferentially to within-network connectivity and heightened degree centrality in the ventral attention network, whereas connectivity within network-linked vertices predicts cognitive ability. This connectivity is associated closely with maturational refinement of cortical organization. Children with low ventral attention network connectivity exhibit adolescent-like topographical profiles, suggesting that attentional systems may be relevant in understanding how brain functions are refined across development. These data suggest a role for attention networks in supporting age-dependent shifts in cortical organization and cognition across childhood and adolescence.

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The development of structural covariance networks during the transition from childhood to adolescence

Functional connectivity development along the sensorimotor-association axis enhances the cortical hierarchy

A shifting role of thalamocortical connectivity in the emergence of cortical functional organization

Data availability.

Data from the CCNP dataset used here are available at CCNP—Lifespan Brain-Mind Development Data Community at Science Data Bank ( https://ccnp.scidb.cn/en ) including both anonymized neuroimaging data ( https://doi.org/10.57760/sciencedb.07860 ) and unthresholded whole-brain connectivity matrices grouped by relevant ages (children and adolescents) ( https://doi.org/10.11922/sciencedb.00886 ). The raw CCNP data are available from the website upon reasonable request. The ABCD data used in this report came from the Annual Release v.2.0 ( https://doi.org/10.15154/1503209 ) of the ABCD BIDS Community Collection (ABCC; NDA Collection 3165). Source data are provided with this paper.

Code availability

Code is available via GitHub: (1) preprocessing CCNP datasets ( https://github.com/zuoxinian/CCS ); (2) preprocessing ABCD datasets ( https://github.com/ThomasYeoLab/ABCD_scripts ); (3) FC gradient analysis ( https://github.com/NeuroanatomyAndConnectivity/gradient_analysis ); and (4) Gradient maturation analysis ( https://github.com/HolmesLab/GradientMaturation ).

Casey, B. J., Heller, A. S., Gee, D. G. & Cohen, A. O. Development of the emotional brain. Neurosci. Lett. 693 , 29–34 (2019).

Article   CAS   PubMed   Google Scholar  

Casey, B. J., Getz, S. & Galvan, A. The adolescent brain. Dev. Rev. 28 , 62–77 (2008).

Article   CAS   PubMed   PubMed Central   Google Scholar  

Luna, B. et al. Maturation of widely distributed brain function subserves cognitive development. Neuroimage 13 , 786–793 (2001).

Kang, H. J. et al. Spatio-temporal transcriptome of the human brain. Nature 478 , 483–489 (2011).

Huttenlocher, P. R. & Dabholkar, A. S. Regional differences in synaptogenesis in human cerebral cortex. J. Comp. Neurol. 387 , 167–178 (1997).

Paquola, C. et al. Shifts in myeloarchitecture characterise adolescent development of cortical gradients. eLife 8 , e50482 (2019).

Zilles, K., Palomero-Gallagher, N. & Amunts, K. Development of cortical folding during evolution and ontogeny. Trends Neurosci. 36 , 275–284 (2013).

Reardon, P. K. et al. Normative brain size variation and brain shape diversity in humans. Science 360 , 1222–1227 (2018).

Bethlehem, R. A. I. et al. Brain charts for the human lifespan. Nature 604 , 525–533 (2022).

Margulies, D. S. et al. Situating the default-mode network along a principal gradient of macroscale cortical organization. Proc. Natl Acad. Sci. USA 113 , 12574–12579 (2016).

Yeo, B. T. et al. The organization of the human cerebral cortex estimated by intrinsic functional connectivity. J. Neurophysiol. 106 , 1125–1165 (2011).

Article   PubMed   Google Scholar  

Gao, W. et al. Temporal and spatial evolution of brain network topology during the first two years of life. PLoS ONE 6 , e25278 (2011).

Fair, D. A. et al. Functional brain networks develop from a ‘Local to Distributed’ organization. PLoS Comput. Biol. 5 , e1000381 (2009).

Article   PubMed   PubMed Central   Google Scholar  

Dong, H. M., Margulies, D. S., Zuo, X. N. & Holmes, A. J. Shifting gradients of macroscale cortical organization mark the transition from childhood to adolescence. Proc. Natl Acad. Sci. USA 118 , e2024448118 (2021).

Somerville, L. H., Hare, T. & Casey, B. J. Frontostriatal maturation predicts cognitive control failure to appetitive cues in adolescents. J. Cogn. Neurosci. 23 , 2123–2134 (2011).

Tottenham, N. & Sheridan, M. A. A review of adversity, the amygdala and the hippocampus: a consideration of developmental timing. Front. Hum. Neurosci. 3 , 68 (2009).

PubMed   Google Scholar  

Sydnor, V. J. et al. Neurodevelopment of the association cortices: patterns, mechanisms, and implications for psychopathology. Neuron 109 , 2820–2846 (2021).

Fair, D. A. et al. Development of distinct control networks through segregation and integration. Proc. Natl Acad. Sci. USA 104 , 13507–13512 (2007).

Betzel, R. F. et al. Changes in structural and functional connectivity among resting-state networks across the human lifespan. Neuroimage 102 , 345–357 (2014).

Betzel, R. F. et al. Generative models of the human connectome. Neuroimage 124 , 1054–1064 (2016).

Zuo, X. N. et al. Human connectomics across the life span. Trends Cogn. Sci. 21 , 32–45 (2017).

Tooley, U. A., Bassett, D. S. & Mackey, A. P. Functional brain network community structure in childhood: Unfinished territories and fuzzy boundaries. Neuroimage 247 , 118843 (2022).

Mesulam, M. M. From sensation to cognition. Brain 121 , 1013–1052 (1998).

Sepulcre, J., Sabuncu, M. R., Yeo, T. B., Liu, H. & Johnson, K. A. Stepwise connectivity of the modal cortex reveals the multimodal organization of the human brain. J. Neurosci. 32 , 10649–10661 (2012).

Power, J. D. et al. Functional network organization of the human brain. Neuron 72 , 665–678 (2011).

Dosenbach, N. U. et al. Distinct brain networks for adaptive and stable task control in humans. Proc. Natl Acad. Sci. USA 104 , 11073–11078 (2007).

Seeley, W. W. et al. Dissociable intrinsic connectivity networks for salience processing and executive control. J. Neurosci. 27 , 2349–2356 (2007).

Dosenbach, N. U., Fair, D. A., Cohen, A. L., Schlaggar, B. L. & Petersen, S. E. A dual-networks architecture of top-down control. Trends Cogn. Sci. 12 , 99–105 (2008).

Dosenbach, N. U. et al. A core system for the implementation of task sets. Neuron 50 , 799–812 (2006).

Labache, L., Ge, T., Yeo, B. T. T. & Holmes, A. J. Language network lateralization is reflected throughout the macroscale functional organization of cortex. Nat. Commun. 14 , 3405 (2023).

Liu, S. et al. Chinese color nest project: an accelerated longitudinal brain-mind cohort. Dev. Cogn. Neurosci. 52 , 101020 (2021).

Alexander-Bloch, A. F. et al. On testing for spatial correspondence between maps of human brain structure and function. Neuroimage 178 , 540–551 (2018).

Schaefer, A. et al. Local-global parcellation of the human cerebral cortex from intrinsic functional connectivity MRI. Cereb. Cortex 28 , 3095–3114 (2018).

Gogtay, N. et al. Dynamic mapping of human cortical development during childhood through early adulthood. Proc. Natl Acad. Sci. USA 101 , 8174–8179 (2004).

Langs, G. et al. Identifying shared brain networks in individuals by decoupling functional and anatomical variability. Cereb. Cortex 26 , 4004–4014 (2016).

Coifman, R. R. & Lafon, S. Diffusion maps. Appl. Comput. Harmon. A 21 , 5–30 (2006).

Article   Google Scholar  

Fan, X. R. et al. A longitudinal resource for population neuroscience of school-age children and adolescents in China. Sci. Data 10 , 545 (2023).

Casey, B. J. et al. The Adolescent Brain Cognitive Development (ABCD) study: imaging acquisition across 21 sites. Dev. Cogn. Neurosci. 32 , 43–54 (2018).

Luciana, M. et al. Adolescent neurocognitive development and impacts of substance use: overview of the adolescent brain cognitive development (ABCD) baseline neurocognition battery. Dev. Cogn. Neurosci. 32 , 67–79 (2018).

Ricard, J. A. et al. Confronting racially exclusionary practices in the acquisition and analyses of neuroimaging data. Nat. Neurosci. 26 , 4–11 (2023).

Li, J. et al. Cross-ethnicity/race generalization failure of behavioral prediction from resting-state functional connectivity. Sci. Adv. 8 , eabj1812 (2022).

Fox, M. D. et al. The human brain is intrinsically organized into dynamic, anticorrelated functional networks. Proc. Natl Acad. Sci. USA 102 , 9673–9678 (2005).

Corbetta, M. & Shulman, G. L. Control of goal-directed and stimulus-driven attention in the brain. Nat. Rev. Neurosci. 3 , 201–215 (2002).

Gordon, E. M. et al. Precision functional mapping of individual human brains. Neuron 95 , 791–807 e797 (2017).

Sridharan, D., Levitin, D. J. & Menon, V. A critical role for the right fronto-insular cortex in switching between central-executive and default-mode networks. Proc. Natl Acad. Sci. USA 105 , 12569–12574 (2008).

Farrant, K. & Uddin, L. Q. Asymmetric development of dorsal and ventral attention networks in the human brain. Dev. Cogn. Neurosci. 12 , 165–174 (2015).

Casey, B. J., Giedd, J. N. & Thomas, K. M. Structural and functional brain development and its relation to cognitive development. Biol. Psychol. 54 , 241–257 (2000).

Molloy, M. F. et al. Effect of extremely preterm birth on adolescent brain network organization. Brain Connect. 13 , 394–409 (2023).

Huizinga, M., Dolan, C. V. & van der Molen, M. W. Age-related change in executive function: developmental trends and a latent variable analysis. Neuropsychologia 44 , 2017–2036 (2006).

Luna, B., Garver, K. E., Urban, T. A., Lazar, N. A. & Sweeney, J. A. Maturation of cognitive processes from late childhood to adulthood. Child Dev. 75 , 1357–1372 (2004).

Gordon, E. M. et al. A somato-cognitive action network alternates with effector regions in motor cortex. Nature 617 , 351–359 (2023).

Pfisterer, U. & Khodosevich, K. Neuronal survival in the brain: neuron type-specific mechanisms. Cell Death Dis. 8 , e2643 (2017).

Bullmore, E. & Sporns, O. The economy of brain network organization. Nat. Rev. Neurosci. 13 , 336–349 (2012).

Gee, D. G. et al. Early developmental emergence of human amygdala-prefrontal connectivity after maternal deprivation. Proc. Natl Acad. Sci. USA 110 , 15638–15643 (2013).

Dong, H. M. et al. Charting brain growth in tandem with brain templates at school age. Sci. Bull. 65 , 1924–1934 (2020).

Yang, N. et al. Chinese color nest project: growing up in China (in Chinese). Chin. Sci. Bull. 62 , 3008–3022 (2017).

Auchter, A. M. et al. A description of the ABCD organizational structure and communication framework. Dev Cogn Neurosci 32 , 8–15 (2018).

Clark, D. B. et al. Biomedical ethics and clinical oversight in multisite observational neuroimaging studies with children and adolescents: the ABCD experience. Dev. Cogn. Neurosci. 32 , 143–154 (2018).

Manjon, J. V. & Coupe, P. volBrain: an online MRI brain volumetry system. Front. Neuroinform. 10 , 30 (2016).

Xu, T. et al. A connectome computation system for discovery science of brain. Sci. Bull. 60 , 86–95 (2015).

Xing, X. X. et al. Connectome computation system: 2015–2021 updates. Sci. Bull. 67 , 448–451 (2022).

Friston, K. J. et al. Statistical parametric maps in functional imaging: a general linear approach. Hum. Brain Mapp. 2 , 189–210 (1994).

Jenkinson, M., Beckmann, C. F., Behrens, T. E., Woolrich, M. W. & Smith, S. M. FSL. Neuroimage 62 , 782–790 (2012).

Cox, R. W. AFNI: software for analysis and visualization of functional magnetic resonance neuroimages. Comput. Biomed. Res. 29 , 162–173 (1996).

Fischl, B. FreeSurfer. Neuroimage 62 , 774–781 (2012).

Pruim, R. H. R. et al. ICA-AROMA: a robust ICA-based strategy for removing motion artifacts from fMRI data. Neuroimage 112 , 267–277 (2015).

Hagler, D. J. Jr. et al. Image processing and analysis methods for the adolescent brain cognitive development study. Neuroimage 202 , 116091 (2019).

Chen, J. et al. Shared and unique brain network features predict cognitive, personality, and mental health scores in the ABCD study. Nat. Commun. 13 , 2217 (2022).

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Acknowledgements

This work was supported by the STI 2030—the major projects of the Brain Science and Brain-Inspired Intelligence Technology (2021ZD0200500 to X.-N.Z.), the National Institute of Mental Health (grants R01MH120080 and R01MH123245 to A.J.H.), the Major Fund for International Collaboration of National Natural Science Foundation of China (81220108014 to X.-N.Z.) and the National Basic Science Data Center ‘Interdisciplinary Brain Database for In vivo Population Imaging’ (ID-BRAIN to X.-N.Z.). B.T.T.Y. is supported by the NUS Yong Loo Lin School of Medicine (NUHSRO/2020/124/TMR/LOA), the Singapore National Medical Research Council (NMRC) LCG (OFLCG19May-0035), NMRC CTG-IIT (CTGIIT23jan-0001), NMRC STaR (STaR20nov-0003), Singapore Ministry of Health (MOH) Centre Grant (CG21APR1009), the Temasek Foundation (TF2223-IMH-01) and the United States NIH (R01MH120080 and R01MH133334). Any opinions, findings and conclusions or recommendations expressed in this material are those of the authors and do not reflect the views of the Singapore NMRC, MOH or Temasek Foundation.

Author information

These authors contributed equally: Avram J. Holmes, Xi-Nian Zuo.

Authors and Affiliations

Department of Psychology, Yale University, New Haven, CT, USA

Hao-Ming Dong, Xi-Han Zhang & Loïc Labache

Centre for Sleep and Cognition and Centre for Translational Magnetic Resonance Research, Yong Loo Lin School of Medicine, Singapore, National University of Singapore, Singapore, Singapore

Shaoshi Zhang, Leon Qi Rong Ooi & B. T. Thomas Yeo

Department of Electrical and Computer Engineering, National University of Singapore, Singapore, Singapore

B. T. Thomas Yeo

N.1 Institute for Health and Institute for Digital Medicine, National University of Singapore, Singapore, Singapore

Centre National de la Recherche Scientifique, Frontlab, Institut du Cerveau et de la Moelle Epinière, Paris, France

Daniel S. Margulies

Department of Psychiatry, Brain Health Institute, Rutgers University, Piscataway, NJ, USA

Avram J. Holmes

State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China

Xi-Nian Zuo

National Basic Science Data Center, Beijing, China

Developmental Population Neuroscience Research Center, IDG/McGovern Institute for Brain Research, Beijing Normal University, Beijing, China

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Contributions

H.-M.D., A.J.H. and X.-N.Z. designed the research. A.J.H. and X.-N.Z. supervised the research. H.-M.D. and A.J.H. conducted analyses and made figures. X.-H.Z., L.L., S.Z., L.Q.R.O. and B.T.T.Y. conducted validation analyses based on the ABCD dataset. H.-M.D., A.J.H. and X.-N.Z. wrote the initial draft. X.-H.Z., L.L., S.Z., L.Q.R.O., B.T.T.Y. and D.S.M. edited the paper.

Corresponding authors

Correspondence to Hao-Ming Dong , Avram J. Holmes or Xi-Nian Zuo .

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Extended data

Extended data fig. 1 the transition from unimodal to transmodal organization revealed by the increasing in percentiles of functional connectome..

The threshold of connectivity matrices was adjusted in children and adolescent group and then redrive the gradients. The results revealed a marked transition in functional connectivity strength from childhood to adolescence. With the 95% threshold retaining the strongest connections, a unimodal organization was evident in both children and adolescents as their primary gradients. However, as additional weaker connections are included in the functional connectome at a 90% threshold, the primary gradients diverged for children and adolescents, revealing a unimodal and transmodal organization, respectively. Yet, with an 85% threshold incorporating even more weaker connections, the primary gradients converged into a transmodal organization for both children and adolescents.

Source data

Extended data fig. 2 gradient maps in low ventral attention connectivity groups derived from longitudinal data..

A set of child participants (n=22) were identified from the low ventral attention group who were also subsequently scanned in their adolescence. Surface maps exhibit a stable adolescent-like gradient architecture in both childhood and adolescence. Their first gradient in childhood is highly correlated (r=0.9429, p<0.01, two-sided spin test) with the first gradient that in their adolescence. A consistent group profile that was also evident when considering their second gradients in both childhood and adolescence (r=0.9353, p<0.01, two-sided spin test).

Extended Data Fig. 3 Gradient maps in high ventral attention connectivity groups derived from longitudinal data.

A set of child participants (n=21) were identified from the high ventral attention group who were also subsequently scanned in their adolescence. Surface maps exhibit a developmentally normative pattern of gradient reversals from their childhood to adolescence. Their first gradient in childhood was highly correlated with their second gradient in adolescence (absolute r=0.9793, p<0.01, two-sided spin test), while their second gradient in childhood were highly correlated with the first gradient in their adolescence (absolute r=0.9748, p<0.01, two-sided spin test).

Extended Data Fig. 4 Gradient maps with functional networks dropped off separately.

Virtual lesion analyses were performed for all the networks respectively. It is revealed that in children group, the drop off of visual, somato/motor, ventral attention and frontoparietal networks generating transmodal organization in the first gradient, while the drop off of dorsal attention, limbic and default networks conserve the unimodal organization in the first gradient. Readers should interpret these maps with caution as functional networks each contain distinct numbers of vertices along the cortical sheet. Accordingly, the direct examination across the canonical networks, is likely biased by their relative sizes.

Supplementary information

Supplementary information.

Supplementary Discussion and Tables 1–17.

Reporting Summary

Source data figs. 1–5 and extended data figs. 1–4.

Degree centrality values, Euclidean values and network-level mean and standard gradient values after dropping off ventral attention network and the null distribution generated by the permutation test. Config file for plotting Chord diagram. Gradient values of High/Low ventral attention groups in the CCNP dataset. Gradient values of high/low ventral attention groups in the ABCD dataset. Gradient values of adolescents and children groups with different percentiles of functional connectome. Gradient values in low ventral attention connectivity groups derived from longitudinal data. Gradient values in high ventral attention connectivity groups derived from longitudinal data. Gradient maps with functional networks dropped off separately.

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Dong, HM., Zhang, XH., Labache, L. et al. Ventral attention network connectivity is linked to cortical maturation and cognitive ability in childhood. Nat Neurosci (2024). https://doi.org/10.1038/s41593-024-01736-x

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Received : 19 May 2023

Accepted : 18 July 2024

Published : 23 August 2024

DOI : https://doi.org/10.1038/s41593-024-01736-x

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