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• How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

• An independent variable is something the researcher changes or controls.
• A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias  will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in  if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

• H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
• H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

• Sampling methods
• Simple random sampling
• Stratified sampling
• Cluster sampling
• Likert scales
• Reproducibility

 Statistics

• Null hypothesis
• Statistical power
• Probability distribution
• Effect size
• Poisson distribution

Research bias

• Optimism bias
• Cognitive bias
• Implicit bias
• Hawthorne effect
• Anchoring bias
• Explicit bias

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A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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Statistics By Jim

Making statistics intuitive

Statistical Hypothesis Testing Overview

By Jim Frost 59 Comments

In this blog post, I explain why you need to use statistical hypothesis testing and help you navigate the essential terminology. Hypothesis testing is a crucial procedure to perform when you want to make inferences about a population using a random sample. These inferences include estimating population properties such as the mean, differences between means, proportions, and the relationships between variables.

This post provides an overview of statistical hypothesis testing. If you need to perform hypothesis tests, consider getting my book, Hypothesis Testing: An Intuitive Guide .

Why You Should Perform Statistical Hypothesis Testing

Hypothesis testing is a form of inferential statistics that allows us to draw conclusions about an entire population based on a representative sample. You gain tremendous benefits by working with a sample. In most cases, it is simply impossible to observe the entire population to understand its properties. The only alternative is to collect a random sample and then use statistics to analyze it.

While samples are much more practical and less expensive to work with, there are trade-offs. When you estimate the properties of a population from a sample, the sample statistics are unlikely to equal the actual population value exactly.  For instance, your sample mean is unlikely to equal the population mean. The difference between the sample statistic and the population value is the sample error.

Differences that researchers observe in samples might be due to sampling error rather than representing a true effect at the population level. If sampling error causes the observed difference, the next time someone performs the same experiment the results might be different. Hypothesis testing incorporates estimates of the sampling error to help you make the correct decision. Learn more about Sampling Error .

For example, if you are studying the proportion of defects produced by two manufacturing methods, any difference you observe between the two sample proportions might be sample error rather than a true difference. If the difference does not exist at the population level, you won’t obtain the benefits that you expect based on the sample statistics. That can be a costly mistake!

Let’s cover some basic hypothesis testing terms that you need to know.

Background information : Difference between Descriptive and Inferential Statistics and Populations, Parameters, and Samples in Inferential Statistics

Hypothesis Testing

Hypothesis testing is a statistical analysis that uses sample data to assess two mutually exclusive theories about the properties of a population. Statisticians call these theories the null hypothesis and the alternative hypothesis. A hypothesis test assesses your sample statistic and factors in an estimate of the sample error to determine which hypothesis the data support.

When you can reject the null hypothesis, the results are statistically significant, and your data support the theory that an effect exists at the population level.

The effect is the difference between the population value and the null hypothesis value. The effect is also known as population effect or the difference. For example, the mean difference between the health outcome for a treatment group and a control group is the effect.

Typically, you do not know the size of the actual effect. However, you can use a hypothesis test to help you determine whether an effect exists and to estimate its size. Hypothesis tests convert your sample effect into a test statistic, which it evaluates for statistical significance. Learn more about Test Statistics .

An effect can be statistically significant, but that doesn’t necessarily indicate that it is important in a real-world, practical sense. For more information, read my post about Statistical vs. Practical Significance .

Null Hypothesis

The null hypothesis is one of two mutually exclusive theories about the properties of the population in hypothesis testing. Typically, the null hypothesis states that there is no effect (i.e., the effect size equals zero). The null is often signified by H 0 .

In all hypothesis testing, the researchers are testing an effect of some sort. The effect can be the effectiveness of a new vaccination, the durability of a new product, the proportion of defect in a manufacturing process, and so on. There is some benefit or difference that the researchers hope to identify.

However, it’s possible that there is no effect or no difference between the experimental groups. In statistics, we call this lack of an effect the null hypothesis. Therefore, if you can reject the null, you can favor the alternative hypothesis, which states that the effect exists (doesn’t equal zero) at the population level.

You can think of the null as the default theory that requires sufficiently strong evidence against in order to reject it.

For example, in a 2-sample t-test, the null often states that the difference between the two means equals zero.

When you can reject the null hypothesis, your results are statistically significant. Learn more about Statistical Significance: Definition & Meaning .

Related post : Understanding the Null Hypothesis in More Detail

Alternative Hypothesis

The alternative hypothesis is the other theory about the properties of the population in hypothesis testing. Typically, the alternative hypothesis states that a population parameter does not equal the null hypothesis value. In other words, there is a non-zero effect. If your sample contains sufficient evidence, you can reject the null and favor the alternative hypothesis. The alternative is often identified with H 1 or H A .

For example, in a 2-sample t-test, the alternative often states that the difference between the two means does not equal zero.

You can specify either a one- or two-tailed alternative hypothesis:

If you perform a two-tailed hypothesis test, the alternative states that the population parameter does not equal the null value. For example, when the alternative hypothesis is H A : μ ≠ 0, the test can detect differences both greater than and less than the null value.

A one-tailed alternative has more power to detect an effect but it can test for a difference in only one direction. For example, H A : μ > 0 can only test for differences that are greater than zero.

Related posts : Understanding T-tests and One-Tailed and Two-Tailed Hypothesis Tests Explained

P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is correct. In simpler terms, p-values tell you how strongly your sample data contradict the null. Lower p-values represent stronger evidence against the null. You use P-values in conjunction with the significance level to determine whether your data favor the null or alternative hypothesis.

Related post : Interpreting P-values Correctly

Significance Level (Alpha)

For instance, a significance level of 0.05 signifies a 5% risk of deciding that an effect exists when it does not exist.

Use p-values and significance levels together to help you determine which hypothesis the data support. If the p-value is less than your significance level, you can reject the null and conclude that the effect is statistically significant. In other words, the evidence in your sample is strong enough to be able to reject the null hypothesis at the population level.

Related posts : Graphical Approach to Significance Levels and P-values and Conceptual Approach to Understanding Significance Levels

Types of Errors in Hypothesis Testing

Statistical hypothesis tests are not 100% accurate because they use a random sample to draw conclusions about entire populations. There are two types of errors related to drawing an incorrect conclusion.

• False positives: You reject a null that is true. Statisticians call this a Type I error . The Type I error rate equals your significance level or alpha (α).
• False negatives: You fail to reject a null that is false. Statisticians call this a Type II error. Generally, you do not know the Type II error rate. However, it is a larger risk when you have a small sample size , noisy data, or a small effect size. The type II error rate is also known as beta (β).

Statistical power is the probability that a hypothesis test correctly infers that a sample effect exists in the population. In other words, the test correctly rejects a false null hypothesis. Consequently, power is inversely related to a Type II error. Power = 1 – β. Learn more about Power in Statistics .

Related posts : Types of Errors in Hypothesis Testing and Estimating a Good Sample Size for Your Study Using Power Analysis

Which Type of Hypothesis Test is Right for You?

There are many different types of procedures you can use. The correct choice depends on your research goals and the data you collect. Do you need to understand the mean or the differences between means? Or, perhaps you need to assess proportions. You can even use hypothesis testing to determine whether the relationships between variables are statistically significant.

To choose the proper statistical procedure, you’ll need to assess your study objectives and collect the correct type of data . This background research is necessary before you begin a study.

Related Post : Hypothesis Tests for Continuous, Binary, and Count Data

Statistical tests are crucial when you want to use sample data to make conclusions about a population because these tests account for sample error. Using significance levels and p-values to determine when to reject the null hypothesis improves the probability that you will draw the correct conclusion.

To see an alternative approach to these traditional hypothesis testing methods, learn about bootstrapping in statistics !

If you want to see examples of hypothesis testing in action, I recommend the following posts that I have written:

• How Effective Are Flu Shots? This example shows how you can use statistics to test proportions.
• Fatality Rates in Star Trek . This example shows how to use hypothesis testing with categorical data.
• Busting Myths About the Battle of the Sexes . A fun example based on a Mythbusters episode that assess continuous data using several different tests.
• Are Yawns Contagious? Another fun example inspired by a Mythbusters episode.

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Reader Interactions

January 14, 2024 at 8:43 am

Hello professor Jim, how are you doing! Pls. What are the properties of a population and their examples? Thanks for your time and understanding.

January 14, 2024 at 12:57 pm

Please read my post about Populations vs. Samples for more information and examples.

Also, please note there is a search bar in the upper-right margin of my website. Use that to search for topics.

July 5, 2023 at 7:05 am

Hello, I have a question as I read your post. You say in p-values section

“P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is correct. In simpler terms, p-values tell you how strongly your sample data contradict the null. Lower p-values represent stronger evidence against the null.”

But according to your definition of effect, the null states that an effect does not exist, correct? So what I assume you want to say is that “P-values are the probability that you would obtain the effect observed in your sample, or larger, if the null hypothesis is **incorrect**.”

July 6, 2023 at 5:18 am

Hi Shrinivas,

The correct definition of p-value is that it is a probability that exists in the context of a true null hypothesis. So, the quotation is correct in stating “if the null hypothesis is correct.”

Essentially, the p-value tells you the likelihood of your observed results (or more extreme) if the null hypothesis is true. It gives you an idea of whether your results are surprising or unusual if there is no effect.

Hence, with sufficiently low p-values, you reject the null hypothesis because it’s telling you that your sample results were unlikely to have occurred if there was no effect in the population.

I hope that helps make it more clear. If not, let me know I’ll attempt to clarify!

May 8, 2023 at 12:47 am

Thanks a lot Ny best regards

May 7, 2023 at 11:15 pm

Hi Jim Can you tell me something about size effect? Thanks

May 8, 2023 at 12:29 am

Here’s a post that I’ve written about Effect Sizes that will hopefully tell you what you need to know. Please read that. Then, if you have any more specific questions about effect sizes, please post them there. Thanks!

January 7, 2023 at 4:19 pm

Hi Jim, I have only read two pages so far but I am really amazed because in few paragraphs you made me clearly understand the concepts of months of courses I received in biostatistics! Thanks so much for this work you have done it helps a lot!

January 10, 2023 at 3:25 pm

Thanks so much!

June 17, 2021 at 1:45 pm

Can you help in the following question: Rocinante36 is priced at ₹7 lakh and has been designed to deliver a mileage of 22 km/litre and a top speed of 140 km/hr. Formulate the null and alternative hypotheses for mileage and top speed to check whether the new models are performing as per the desired design specifications.

April 19, 2021 at 1:51 pm

Its indeed great to read your work statistics.

I have a doubt regarding the one sample t-test. So as per your book on hypothesis testing with reference to page no 45, you have mentioned the difference between “the sample mean and the hypothesised mean is statistically significant”. So as per my understanding it should be quoted like “the difference between the population mean and the hypothesised mean is statistically significant”. The catch here is the hypothesised mean represents the sample mean.

Please help me understand this.

Regards Rajat

April 19, 2021 at 3:46 pm

Thanks for buying my book. I’m so glad it’s been helpful!

The test is performed on the sample but the results apply to the population. Hence, if the difference between the sample mean (observed in your study) and the hypothesized mean is statistically significant, that suggests that population does not equal the hypothesized mean.

For one sample tests, the hypothesized mean is not the sample mean. It is a mean that you want to use for the test value. It usually represents a value that is important to your research. In other words, it’s a value that you pick for some theoretical/practical reasons. You pick it because you want to determine whether the population mean is different from that particular value.

I hope that helps!

November 5, 2020 at 6:24 am

Jim, you are such a magnificent statistician/economist/econometrician/data scientist etc whatever profession. Your work inspires and simplifies the lives of so many researchers around the world. I truly admire you and your work. I will buy a copy of each book you have on statistics or econometrics. Keep doing the good work. Remain ever blessed

November 6, 2020 at 9:47 pm

Hi Renatus,

Thanks so much for you very kind comments. You made my day!! I’m so glad that my website has been helpful. And, thanks so much for supporting my books! 🙂

November 2, 2020 at 9:32 pm

Hi Jim, I hope you are aware of 2019 American Statistical Association’s official statement on Statistical Significance: https://www.tandfonline.com/doi/full/10.1080/00031305.2019.1583913 In case you do not bother reading the full article, may I quote you the core message here: “We conclude, based on our review of the articles in this special issue and the broader literature, that it is time to stop using the term “statistically significant” entirely. Nor should variants such as “significantly different,” “p < 0.05,” and “nonsignificant” survive, whether expressed in words, by asterisks in a table, or in some other way."

With best wishes,

November 3, 2020 at 2:09 am

I’m definitely aware of the debate surrounding how to use p-values most effectively. However, I need to correct you on one point. The link you provide is NOT a statement by the American Statistical Association. It is an editorial by several authors.

There is considerable debate over this issue. There are problems with p-values. However, as the authors state themselves, much of the problem is over people’s mindsets about how to use p-values and their incorrect interpretations about what statistical significance does and does not mean.

If you were to read my website more thoroughly, you’d be aware that I share many of their concerns and I address them in multiple posts. One of the authors’ key points is the need to be thoughtful and conduct thoughtful research and analysis. I emphasize this aspect in multiple posts on this topic. I’ll ask you to read the following three because they all address some of the authors’ concerns and suggestions. But you might run across others to read as well.

Five Tips for Using P-values to Avoid Being Misled How to Interpret P-values Correctly P-values and the Reproducibility of Experimental Results

September 24, 2020 at 11:52 pm

HI Jim, i just want you to know that you made explanation for Statistics so simple! I should say lesser and fewer words that reduce the complexity. All the best! 🙂

September 25, 2020 at 1:03 am

Thanks, Rene! Your kind words mean a lot to me! I’m so glad it has been helpful!

September 23, 2020 at 2:21 am

Honestly, I never understood stats during my entire M.Ed course and was another nightmare for me. But how easily you have explained each concept, I have understood stats way beyond my imagination. Thank you so much for helping ignorant research scholars like us. Looking forward to get hardcopy of your book. Kindly tell is it available through flipkart?

September 24, 2020 at 11:14 pm

I’m so happy to hear that my website has been helpful!

I checked on flipkart and it appears like my books are not available there. I’m never exactly sure where they’re available due to the vagaries of different distribution channels. They are available on Amazon in India.

Introduction to Statistics: An Intuitive Guide (Amazon IN) Hypothesis Testing: An Intuitive Guide (Amazon IN)

July 26, 2020 at 11:57 am

Dear Jim I am a teacher from India . I don’t have any background in statistics, and still I should tell that in a single read I can follow your explanations . I take my entire biostatistics class for botany graduates with your explanations. Thanks a lot. May I know how I can avail your books in India

July 28, 2020 at 12:31 am

Right now my books are only available as ebooks from my website. However, soon I’ll have some exciting news about other ways to obtain it. Stay tuned! I’ll announce it on my email list. If you’re not already on it, you can sign up using the form that is in the right margin of my website.

June 22, 2020 at 2:02 pm

Also can you please let me if this book covers topics like EDA and principal component analysis?

June 22, 2020 at 2:07 pm

This book doesn’t cover principal components analysis. Although, I wouldn’t really classify that as a hypothesis test. In the future, I might write a multivariate analysis book that would cover this and others. But, that’s well down the road.

My Introduction to Statistics covers EDA. That’s the largely graphical look at your data that you often do prior to hypothesis testing. The Introduction book perfectly leads right into the Hypothesis Testing book.

June 22, 2020 at 1:45 pm

Thanks for the detailed explanation. It does clear my doubts. I saw that your book related to hypothesis testing has the topics that I am studying currently. I am looking forward to purchasing it.

Regards, Take Care

June 19, 2020 at 1:03 pm

For this particular article I did not understand a couple of statements and it would great if you could help: 1)”If sample error causes the observed difference, the next time someone performs the same experiment the results might be different.” 2)”If the difference does not exist at the population level, you won’t obtain the benefits that you expect based on the sample statistics.”

I discovered your articles by chance and now I keep coming back to read & understand statistical concepts. These articles are very informative & easy to digest. Thanks for the simplifying things.

June 20, 2020 at 9:53 pm

I’m so happy to hear that you’ve found my website to be helpful!

To answer your questions, keep in mind that a central tenant of inferential statistics is that the random sample that a study drew was only one of an infinite number of possible it could’ve drawn. Each random sample produces different results. Most results will cluster around the population value assuming they used good methodology. However, random sampling error always exists and makes it so that population estimates from a sample almost never exactly equal the correct population value.

So, imagine that we’re studying a medication and comparing the treatment and control groups. Suppose that the medicine is truly not effect and that the population difference between the treatment and control group is zero (i.e., no difference.) Despite the true difference being zero, most sample estimates will show some degree of either a positive or negative effect thanks to random sampling error. So, just because a study has an observed difference does not mean that a difference exists at the population level. So, on to your questions:

1. If the observed difference is just random error, then it makes sense that if you collected another random sample, the difference could change. It could change from negative to positive, positive to negative, more extreme, less extreme, etc. However, if the difference exists at the population level, most random samples drawn from the population will reflect that difference. If the medicine has an effect, most random samples will reflect that fact and not bounce around on both sides of zero as much.

2. This is closely related to the previous answer. If there is no difference at the population level, but say you approve the medicine because of the observed effects in a sample. Even though your random sample showed an effect (which was really random error), that effect doesn’t exist. So, when you start using it on a larger scale, people won’t benefit from the medicine. That’s why it’s important to separate out what is easily explained by random error versus what is not easily explained by it.

I think reading my post about how hypothesis tests work will help clarify this process. Also, in about 24 hours (as I write this), I’ll be releasing my new ebook about Hypothesis Testing!

May 29, 2020 at 5:23 am

Hi Jim, I really enjoy your blog. Can you please link me on your blog where you discuss about Subgroup analysis and how it is done? I need to use non parametric and parametric statistical methods for my work and also do subgroup analysis in order to identify potential groups of patients that may benefit more from using a treatment than other groups.

May 29, 2020 at 2:12 pm

Hi, I don’t have a specific article about subgroup analysis. However, subgroup analysis is just the dividing up of a larger sample into subgroups and then analyzing those subgroups separately. You can use the various analyses I write about on the subgroups.

Alternatively, you can include the subgroups in regression analysis as an indicator variable and include that variable as a main effect and an interaction effect to see how the relationships vary by subgroup without needing to subdivide your data. I write about that approach in my article about comparing regression lines . This approach is my preferred approach when possible.

April 19, 2020 at 7:58 am

sir is confidence interval is a part of estimation?

April 17, 2020 at 3:36 pm

Sir can u plz briefly explain alternatives of hypothesis testing? I m unable to find the answer

April 18, 2020 at 1:22 am

Assuming you want to draw conclusions about populations by using samples (i.e., inferential statistics ), you can use confidence intervals and bootstrap methods as alternatives to the traditional hypothesis testing methods.

March 9, 2020 at 10:01 pm

Hi JIm, could you please help with activities that can best teach concepts of hypothesis testing through simulation, Also, do you have any question set that would enhance students intuition why learning hypothesis testing as a topic in introductory statistics. Thanks.

March 5, 2020 at 3:48 pm

Hi Jim, I’m studying multiple hypothesis testing & was wondering if you had any material that would be relevant. I’m more trying to understand how testing multiple samples simultaneously affects your results & more on the Bonferroni Correction

March 5, 2020 at 4:05 pm

I write about multiple comparisons (aka post hoc tests) in the ANOVA context . I don’t talk about Bonferroni Corrections specifically but I cover related types of corrections. I’m not sure if that exactly addresses what you want to know but is probably the closest I have already written. I hope it helps!

January 14, 2020 at 9:03 pm

Thank you! Have a great day/evening.

January 13, 2020 at 7:10 pm

Any help would be greatly appreciated. What is the difference between The Hypothesis Test and The Statistical Test of Hypothesis?

January 14, 2020 at 11:02 am

They sound like the same thing to me. Unless this is specialized terminology for a particular field or the author was intending something specific, I’d guess they’re one and the same.

April 1, 2019 at 10:00 am

so these are the only two forms of Hypothesis used in statistical testing?

April 1, 2019 at 10:02 am

Are you referring to the null and alternative hypothesis? If so, yes, that’s those are the standard hypotheses in a statistical hypothesis test.

April 1, 2019 at 9:57 am

year very insightful post, thanks for the write up

October 27, 2018 at 11:09 pm

hi there, am upcoming statistician, out of all blogs that i have read, i have found this one more useful as long as my problem is concerned. thanks so much

October 27, 2018 at 11:14 pm

Hi Stano, you’re very welcome! Thanks for your kind words. They mean a lot! I’m happy to hear that my posts were able to help you. I’m sure you will be a fantastic statistician. Best of luck with your studies!

October 26, 2018 at 11:39 am

Dear Jim, thank you very much for your explanations! I have a question. Can I use t-test to compare two samples in case each of them have right bias?

October 26, 2018 at 12:00 pm

Hi Tetyana,

You’re very welcome!

The term “right bias” is not a standard term. Do you by chance mean right skewed distributions? In other words, if you plot the distribution for each group on a histogram they have longer right tails? These are not the symmetrical bell-shape curves of the normal distribution.

If that’s the case, yes you can as long as you exceed a specific sample size within each group. I include a table that contains these sample size requirements in my post about nonparametric vs parametric analyses .

Bias in statistics refers to cases where an estimate of a value is systematically higher or lower than the true value. If this is the case, you might be able to use t-tests, but you’d need to be sure to understand the nature of the bias so you would understand what the results are really indicating.

I hope this helps!

April 2, 2018 at 7:28 am

Simple and upto the point 👍 Thank you so much.

April 2, 2018 at 11:11 am

Hi Kalpana, thanks! And I’m glad it was helpful!

March 26, 2018 at 8:41 am

Am I correct if I say: Alpha – Probability of wrongly rejection of null hypothesis P-value – Probability of wrongly acceptance of null hypothesis

March 28, 2018 at 3:14 pm

You’re correct about alpha. Alpha is the probability of rejecting the null hypothesis when the null is true.

Unfortunately, your definition of the p-value is a bit off. The p-value has a fairly convoluted definition. It is the probability of obtaining the effect observed in a sample, or more extreme, if the null hypothesis is true. The p-value does NOT indicate the probability that either the null or alternative is true or false. Although, those are very common misinterpretations. To learn more, read my post about how to interpret p-values correctly .

March 2, 2018 at 6:10 pm

I recently started reading your blog and it is very helpful to understand each concept of statistical tests in easy way with some good examples. Also, I recommend to other people go through all these blogs which you posted. Specially for those people who have not statistical background and they are facing to many problems while studying statistical analysis.

Thank you for your such good blogs.

March 3, 2018 at 10:12 pm

Hi Amit, I’m so glad that my blog posts have been helpful for you! It means a lot to me that you took the time to write such a nice comment! Also, thanks for recommending by blog to others! I try really hard to write posts about statistics that are easy to understand.

January 17, 2018 at 7:03 am

I recently started reading your blog and I find it very interesting. I am learning statistics by my own, and I generally do many google search to understand the concepts. So this blog is quite helpful for me, as it have most of the content which I am looking for.

January 17, 2018 at 3:56 pm

Hi Shashank, thank you! And, I’m very glad to hear that my blog is helpful!

January 2, 2018 at 2:28 pm

thank u very much sir.

January 2, 2018 at 2:36 pm

You’re very welcome, Hiral!

November 21, 2017 at 12:43 pm

Thank u so much sir….your posts always helps me to be a #statistician

November 21, 2017 at 2:40 pm

Hi Sachin, you’re very welcome! I’m happy that you find my posts to be helpful!

November 19, 2017 at 8:22 pm

great post as usual, but it would be nice to see an example.

November 19, 2017 at 8:27 pm

Thank you! At the end of this post, I have links to four other posts that show examples of hypothesis tests in action. You’ll find what you’re looking for in those posts!

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What is a Hypothesis – Types, Examples and Writing Guide

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Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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What Is Hypothesis Testing?

• How It Works

4 Step Process

The bottom line.

• Fundamental Analysis

Hypothesis Testing: 4 Steps and Example

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population or a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, intending to provide evidence on the plausibility of the null hypothesis. Statistical analysts measure and examine a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis. Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

• State the hypotheses.
• Formulate an analysis plan, which outlines how the data will be evaluated.
• Carry out the plan and analyze the sample data.
• Analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Example of Hypothesis Testing

If an individual wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct. Mathematically, the null hypothesis is represented as Ho: P = 0.5. The alternative hypothesis is shown as "Ha" and is identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

When Did Hypothesis Testing Begin?

Some statisticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

Hypothesis testing refers to a statistical process that helps researchers determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. All hypothesis testing methods have the same four-step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

Sage. " Introduction to Hypothesis Testing ," Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples ."

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Hypothesis Testing – A Deep Dive into Hypothesis Testing, The Backbone of Statistical Inference

• September 21, 2023

Explore the intricacies of hypothesis testing, a cornerstone of statistical analysis. Dive into methods, interpretations, and applications for making data-driven decisions.

In this Blog post we will learn:

• What is Hypothesis Testing?
• Steps in Hypothesis Testing 2.1. Set up Hypotheses: Null and Alternative 2.2. Choose a Significance Level (α) 2.3. Calculate a test statistic and P-Value 2.4. Make a Decision
• Example : Testing a new drug.
• Example in python

1. What is Hypothesis Testing?

In simple terms, hypothesis testing is a method used to make decisions or inferences about population parameters based on sample data. Imagine being handed a dice and asked if it’s biased. By rolling it a few times and analyzing the outcomes, you’d be engaging in the essence of hypothesis testing.

Think of hypothesis testing as the scientific method of the statistics world. Suppose you hear claims like “This new drug works wonders!” or “Our new website design boosts sales.” How do you know if these statements hold water? Enter hypothesis testing.

2. Steps in Hypothesis Testing

• Set up Hypotheses : Begin with a null hypothesis (H0) and an alternative hypothesis (Ha).
• Choose a Significance Level (α) : Typically 0.05, this is the probability of rejecting the null hypothesis when it’s actually true. Think of it as the chance of accusing an innocent person.
• Calculate Test statistic and P-Value : Gather evidence (data) and calculate a test statistic.
• p-value : This is the probability of observing the data, given that the null hypothesis is true. A small p-value (typically ≤ 0.05) suggests the data is inconsistent with the null hypothesis.
• Decision Rule : If the p-value is less than or equal to α, you reject the null hypothesis in favor of the alternative.

2.1. Set up Hypotheses: Null and Alternative

Before diving into testing, we must formulate hypotheses. The null hypothesis (H0) represents the default assumption, while the alternative hypothesis (H1) challenges it.

For instance, in drug testing, H0 : “The new drug is no better than the existing one,” H1 : “The new drug is superior .”

2.2. Choose a Significance Level (α)

When You collect and analyze data to test H0 and H1 hypotheses. Based on your analysis, you decide whether to reject the null hypothesis in favor of the alternative, or fail to reject / Accept the null hypothesis.

The significance level, often denoted by $α$, represents the probability of rejecting the null hypothesis when it is actually true.

In other words, it’s the risk you’re willing to take of making a Type I error (false positive).

Type I Error (False Positive) :

• Symbolized by the Greek letter alpha (α).
• Occurs when you incorrectly reject a true null hypothesis . In other words, you conclude that there is an effect or difference when, in reality, there isn’t.
• The probability of making a Type I error is denoted by the significance level of a test. Commonly, tests are conducted at the 0.05 significance level , which means there’s a 5% chance of making a Type I error .
• Commonly used significance levels are 0.01, 0.05, and 0.10, but the choice depends on the context of the study and the level of risk one is willing to accept.

Example : If a drug is not effective (truth), but a clinical trial incorrectly concludes that it is effective (based on the sample data), then a Type I error has occurred.

Type II Error (False Negative) :

• Symbolized by the Greek letter beta (β).
• Occurs when you accept a false null hypothesis . This means you conclude there is no effect or difference when, in reality, there is.
• The probability of making a Type II error is denoted by β. The power of a test (1 – β) represents the probability of correctly rejecting a false null hypothesis.

Example : If a drug is effective (truth), but a clinical trial incorrectly concludes that it is not effective (based on the sample data), then a Type II error has occurred.

Balancing the Errors :

In practice, there’s a trade-off between Type I and Type II errors. Reducing the risk of one typically increases the risk of the other. For example, if you want to decrease the probability of a Type I error (by setting a lower significance level), you might increase the probability of a Type II error unless you compensate by collecting more data or making other adjustments.

It’s essential to understand the consequences of both types of errors in any given context. In some situations, a Type I error might be more severe, while in others, a Type II error might be of greater concern. This understanding guides researchers in designing their experiments and choosing appropriate significance levels.

2.3. Calculate a test statistic and P-Value

Test statistic : A test statistic is a single number that helps us understand how far our sample data is from what we’d expect under a null hypothesis (a basic assumption we’re trying to test against). Generally, the larger the test statistic, the more evidence we have against our null hypothesis. It helps us decide whether the differences we observe in our data are due to random chance or if there’s an actual effect.

P-value : The P-value tells us how likely we would get our observed results (or something more extreme) if the null hypothesis were true. It’s a value between 0 and 1. – A smaller P-value (typically below 0.05) means that the observation is rare under the null hypothesis, so we might reject the null hypothesis. – A larger P-value suggests that what we observed could easily happen by random chance, so we might not reject the null hypothesis.

2.4. Make a Decision

Relationship between $α$ and P-Value

When conducting a hypothesis test:

• We first choose a significance level ($α$), which sets a threshold for making decisions.

We then calculate the p-value from our sample data and the test statistic.

Finally, we compare the p-value to our chosen $α$:

• If $p−value≤α$: We reject the null hypothesis in favor of the alternative hypothesis. The result is said to be statistically significant.
• If $p−value>α$: We fail to reject the null hypothesis. There isn’t enough statistical evidence to support the alternative hypothesis.

3. Example : Testing a new drug.

Imagine we are investigating whether a new drug is effective at treating headaches faster than drug B.

Setting Up the Experiment : You gather 100 people who suffer from headaches. Half of them (50 people) are given the new drug (let’s call this the ‘Drug Group’), and the other half are given a sugar pill, which doesn’t contain any medication.

• Set up Hypotheses : Before starting, you make a prediction:
• Null Hypothesis (H0): The new drug has no effect. Any difference in healing time between the two groups is just due to random chance.
• Alternative Hypothesis (H1): The new drug does have an effect. The difference in healing time between the two groups is significant and not just by chance.
• Choose a Significance Level (α) : Typically 0.05, this is the probability of rejecting the null hypothesis when it’s actually true

Calculate Test statistic and P-Value : After the experiment, you analyze the data. The “test statistic” is a number that helps you understand the difference between the two groups in terms of standard units.

For instance, let’s say:

• The average healing time in the Drug Group is 2 hours.
• The average healing time in the Placebo Group is 3 hours.

The test statistic helps you understand how significant this 1-hour difference is. If the groups are large and the spread of healing times in each group is small, then this difference might be significant. But if there’s a huge variation in healing times, the 1-hour difference might not be so special.

Imagine the P-value as answering this question: “If the new drug had NO real effect, what’s the probability that I’d see a difference as extreme (or more extreme) as the one I found, just by random chance?”

For instance:

• P-value of 0.01 means there’s a 1% chance that the observed difference (or a more extreme difference) would occur if the drug had no effect. That’s pretty rare, so we might consider the drug effective.
• P-value of 0.5 means there’s a 50% chance you’d see this difference just by chance. That’s pretty high, so we might not be convinced the drug is doing much.
• If the P-value is less than ($α$) 0.05: the results are “statistically significant,” and they might reject the null hypothesis , believing the new drug has an effect.
• If the P-value is greater than ($α$) 0.05: the results are not statistically significant, and they don’t reject the null hypothesis , remaining unsure if the drug has a genuine effect.

4. Example in python

For simplicity, let’s say we’re using a t-test (common for comparing means). Let’s dive into Python:

Making a Decision : “The results are statistically significant! p-value < 0.05 , The drug seems to have an effect!” If not, we’d say, “Looks like the drug isn’t as miraculous as we thought.”

5. Conclusion

Hypothesis testing is an indispensable tool in data science, allowing us to make data-driven decisions with confidence. By understanding its principles, conducting tests properly, and considering real-world applications, you can harness the power of hypothesis testing to unlock valuable insights from your data.

More Articles

F statistic formula – explained, correlation – connecting the dots, the role of correlation in data analysis, sampling and sampling distributions – a comprehensive guide on sampling and sampling distributions, law of large numbers – a deep dive into the world of statistics, central limit theorem – a deep dive into central limit theorem and its significance in statistics, similar articles, complete introduction to linear regression in r, how to implement common statistical significance tests and find the p value, logistic regression – a complete tutorial with examples in r.

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Hypothesis tests #

Formal hypothesis testing is perhaps the most prominent and widely-employed form of statistical analysis. It is sometimes seen as the most rigorous and definitive part of a statistical analysis, but it is also the source of many statistical controversies. The currently-prevalent approach to hypothesis testing dates to developments that took place between 1925 and 1940, especially the work of Ronald Fisher , Jerzy Neyman , and Egon Pearson .

In recent years, many prominent statisticians have argued that less emphasis should be placed on the formal hypothesis testing approaches developed in the early twentieth century, with a correspondingly greater emphasis on other forms of uncertainty analysis. Our goal here is to give an overview of some of the well-established and widely-used approaches for hypothesis testing. We will also provide some perspectives on how these tools can be effectively used, and discuss their limitations. We will also discuss some new approaches to hypothesis testing that may eventually come to be as prominent as these classical approaches.

A falsifiable hypothesis is a statement, or hypothesis, that can be contradicted with evidence. In empirical (data-driven) research, this evidence will always be obtained through the data. In statistical hypothesis testing, the hypothesis that we formally test is called the null hypothesis . The alternative hypothesis is a second hypothesis that is our proposed explanation for what happens if the null hypothesis is wrong.

Test statistics #

The key element of a statistical hypothesis test is the test statistic , which (like any statistic) is a function of the data. A test statistic takes our entire dataset, and reduces it to one number. This one number ideally should contain all the information in the data that is relevant for assessing the two hypotheses of interest, and exclude any aspects of the data that are irrelevant for assessing the two hypotheses. The test statistic measures evidence against the null hypothesis. Most test statistics are constructed so that a value of zero represents the lowest possible level of evidence against the null hypothesis. Test statistic values that deviate from zero represent greater levels of evidence against the null hypothesis. The larger the magnitude of the test statistic, the stronger the evidence against the null hypothesis.

A major theme of statistical research is to devise effective ways to construct test statistics. Many useful ways to do this have been devised, and there is no single approach that is always the best. In this introductory course, we will focus on tests that starting with an estimate of a quantity that is relevant for assessing the hypotheses, then proceed by standardizing this estimate by dividing it by its standard error. This approach is sometimes referred to as “Wald testing”, after Abraham Wald .

Testing the equality of two proportions #

As a basic example, let’s consider risk perception related to COVID-19. As you will see below, hypothesis testing can appear at first to be a fairly elaborate exercise. Using this example, we describe each aspect of this exercise in detail below.

The data and research question #

The data shown below are simulated but are designed to reflect actual surveys conducted in the United States in March of 2020. Partipants were asked whether they perceive that they have a substantial risk of dying if they are infected with the novel coronavirus. The number of people stating each response, stratified on age, are shown below (only two age groups are shown):

Each subject’s response is binary – they either perceive themselves to be high risk, or not to be at high risk. When working with this type of data, we are usually interested in the proportion of people who provide each response within each stratum (age group). These are conditional proportions, conditioning on the age group. The numerical values of the conditional proportions are given below:

There are four conditional proportions in the table above – the proportion of younger people who perceive themselves to be at higher risk, 0.110=25/(25+202); the proportion of younger people who do not perceive themselves to be at high risk, 0.890=202/(25+202); the proportion of older people who perceive themselves to be at high risk 0.195=30/(30+124); and the proportion of older people who do not perceive themselves to be at high risk, 0.805=124/(30+124).

The trend in the data is that younger people perceive themselves to be at lower risk of dying than older people, by a difference of 0.195-0.110=0.085 (in terms of proportions). But is this trend only present in this sample, or is it generalizable to a broader population (say the entire US population)? That is the goal of conducting a statistical hypothesis test in this setting.

The population structure #

Corresponding to our data above is the unobserved population structure, which we can denote as follows

The symbols \(p\) and \(q\) in the table above are population parameters . These are quantitites that we do not know, and wish to assess using the data. In this case, our null hypothesis can be expressed as the statement \(p = q\) . We can estimate \(p\) using the sample proportion \(\hat{p} = 0.110\) , and similarly estimate \(q\) using \(\hat{q} = 0.195\) . However these estimates do not immediately provide us with a way of expressing the evidence relating to the hypothesis that \(p=q\) . This is provided by the test statistic.

A test statistic #

As noted above, a test statistic is a reduction of the data to one number that captures all of the relevant information for assessing the hypotheses. A natural first choice for a test statistic here would be the difference in sample proportions between the two age groups, which is 0.195 - 0.110 = 0.085. There is a difference of 0.085 between the perceived risks of death in the younger and older age groups.

The difference in rates (0.085) does not on its own make a good test statistic, although it is a good start toward obtaining one. The reason for this is that the evidence underlying this difference in rates depends also on the absolute rates (0.110 and 0.195), and on the sample sizes (227 and 154). If we only know that the difference in rates is 0.085, this is not sufficient to evaluate the hypothesis in a statistical manner. A given difference in rates is much stronger evidence if it is obtained from a larger sample. If we have a difference of 0.085 with a very large sample, say one million people, then we should be almost certain that the true rates differ (i.e. the data are highly incompatiable with the hypothesis that \(p=q\) ). If we have the same difference in rates of 0.085, but with a small sample, say 50 people per age group, then there would be almost no evidence for a true difference in the rates (i.e. the data are compatiable with the hypothesis \(p=q\) ).

To address this issue, we need to consider the uncertainty in the estimated rate difference, which is 0.085. Recall that the estimated rate difference is obtained from the sample and therefore is almost certain to deviate somewhat from the true rate difference in the population (which is unknown). Recall from our study of standard errors that the standard error for an estimated proportion is \(\sqrt{p(1-p)/n}\) , where \(p\) is the outcome probability (here the outcome is that a person perceives a high risk of dying), and \(n\) is the sample size.

In the present analysis, we are comparing two proportions, so we have two standard errors. The estimated standard error for the younger people is \(\sqrt{0.11\cdot 0.89/227} \approx 0.021\) . The estimated standard error for the older people is \(\sqrt{0.195\cdot 0.805/154} \approx 0.032\) . Note that both standard errors are estimated, rather than exact, because we are plugging in estimates of the rates (0.11 and 0.195). Also note that the standard error for the rate among older people is greater than that for younger people. This is because the sample size for older people is smaller, and also because the estimated rate for older people is closer to 1/2.

In our previous discussion of standard errors, we saw how standard errors for independent quantities \(A\) and \(B\) can be used to obtain the standard error for the difference \(A-B\) . Applying that result here, we see that the standard error for the estimated difference in rates 0.195-0.11=0.085 is \(\sqrt{0.021^2 + 0.032^2} \approx 0.038\) .

The final step in constructing our test statistic is to construct a Z-score from the estimated difference in rates. As with all Z-scores, we proceed by taking the estimated difference in rates, and then divide it by its standard error. Thus, we get a test statistic value of \(0.085 / 0.038 \approx 2.24\) .

A test statistic value of 2.24 is not very close to zero, so there is some evidence against the null hypothesis. But the strength of this evidence remains unclear. Thus, we must consider how to calibrate this evidence in a way that makes it more interpretable.

Calibrating the evidence in the test statistic #

By the central limit theorem (CLT), a Z-score approximately follows a normal distribution. When the null hypothesis holds, the Z-score approximately follows the standard normal distribution (recall that a standard normal distribution is a normal distribution with expected value equal to 0 and variance equal to 1). If the null hypothesis does not hold, then the test statistic continues to approximately follow a normal distribution, but it is not the standard normal distribution.

A test statistic of zero represents the least possible evidence against the null hypothesis. Here, we will obtain a test statistic of zero when the two proportions being compared are identical, i.e. exactly the same proportions of younger and older people perceive a substantial risk of dying from a disease. Even if the test statistic is exactly zero, this does not guarantee that the null hypothesis is true. However it is the least amount of evidence that the data can present against the null hypothesis.

In a hypothesis testing setting using normally-distrbuted Z-scores, as is the case here (due to the CLT), the standard normal distribution is the reference distribution for our test statistic. If the Z-score falls in the center of the reference distribution, there is no evidence against the null hypothesis. If the Z-score falls into either tail of the reference distribution, then there is evidence against the null distribution, and the further into the tails of the reference distribution the Z-score falls, the greater the evidence.

The most conventional way to quantify the evidence in our test statistic is through a probability called the p-value . The p-value has a somewhat complex definition that many people find difficult to grasp. It is the probability of observing as much or more evidence against the null hypothesis as we actually observe, calculated when the null hypothesis is assumed to be true. We will discuss some ways to think about this more intuitively below.

For our purposes, “evidence against the null hypothesis” is reflected in how far into the tails of the reference distribution the Z-score (test statistic) falls. We observed a test statistic of 2.24 in our COVID risk perception analysis. Recall that due to the “empirical rule”, 95% of the time, a draw from a standard normal distribution falls between -2 and 2. Thus, the p-value must be less than 0.05, since 2.24 falls outside this interval. The p-value can be calculated using a computer, in this case it happens to be approximately 0.025.

As stated above, the p-value tells us how likely it would be for us to obtain as much evidence against the the null hypothesis as we observed in our actual data analysis, if we were certain that the null hypothesis were true. When the null hypothesis holds, any evidence against the null hypothesis is spurious. Thus, we will want to see stronger evidence against the null from our actual analysis than we would see if we know that the null hypothesis were true. A smaller p-value therefore reflects more evidence against the null hypothesis than a larger p-value.

By convention, p-values of 0.05 or smaller are considered to represent sufficiently strong evidence against the null hypothesis to make a finding “statistically significant”. This threshold of 0.05 was chosen arbitrarily 100 years ago, and there is no objective reason for it. In recent years, people have argued that either a lesser or a greater p-value threshold should be used. But largely due to convention, the practice of deeming p-values smaller than 0.05 to be statistically significant continues.

Summary of this example #

Here is a restatement of the above discussion, using slightly different language. In our analysis of COVID risk perceptions, we found a difference in proportions of 0.085 between younger and older subjects, with younger people perceiving a lower risk of dying. This is a difference based on the sample of data that we observed, but what we really want to know is whether there is a difference in COVID risk perception in the population (say, all US adults).

Suppose that in fact there is no difference in risk perception between younger and older people. For instance, suppose that in the population, 15% of people believe that they have a substantial risk of dying should they become infected with the novel coronavirus, regardless of their age. Even though the rates are equal in this imaginary population (both being 15%), the rates in our sample would typically not be equal. Around 3% of the time (0.024=2.4% to be exact), if the rates are actually equal in the population, we would see a test statistic that is 2.4 or larger. Since 3% represents a fairly rare event, we can conclude that our observed data are not compatible with the null hypothesis. We can also say that there is statistically significant evidence against the null hypothesis, and that we have “rejected” the null hypothesis at the 3% level.

In this data analysis, as in any data analysis, we cannot confirm definitively that the alternative hypothesis is true. But based on our data and the analysis performed above, we can claim that there is substantial evidence against the null hypothesis, using standard criteria for what is considered to be “substantial evidence”.

Comparison of means #

A very common setting where hypothesis testing is used arises when we wish to compare the means of a quantitative measurement obtained for two populations. Imagine, for example, that we have two ways of manufacturing a battery, and we wish to assess which approach yields batteries that are longer-lasting in actual use. To do this, suppose we obtain data that tells us the number of charge cycles that were completed in 200 batteries of type A, and in 300 batteries of type B. For the test developed below to be meaningful, the data must be independent and identically distributed samples.

The raw data for this study consists of 500 numbers, but it turns out that the most relevant information from the data is contained in the sample means and sample standard deviations computed within each battery type. Note that this is a huge reduction in complexity, since we started with 500 measurements and are able to summarize this down to just four numbers.

Suppose the summary statistics are as follows, where \(\bar{x}\) , \(\hat{\sigma}_x\) , and \(n\) denote the sample mean, sample standard deviation, and sample size, respectively.

The simplest measure comparing the two manufacturing approaches is the difference 420 - 403 = 17. That is, batteries of type A tend to have 17 more charge cycles compared to batteries of type B. This difference is present in our sample, but is it also true that the entire population of type A batteries has more charge cycles than the entire population of type B batteries? That is the goal of conducting a hypothesis test.

The next step in the present analysis is to divide the mean difference, which is 17, by its standard error. As we have seen, the standard error of the mean, or SEM, is \(\sigma/n\) , where \(\sigma\) is the standard deviation and \(n\) is the sample size. Since \(\sigma\) is almost never known, we plug in its estimate \(\hat{\sigma}\) . For the type A batteries, the estimated SEM is thus \(70/\sqrt{200} \approx 4.95\) , and for the type B batteries the estimated SEM is \(90/\sqrt{300} \approx 5.2\) .

Since we are comparing two estimated means that are obtained from independent samples, we can pool the standard deviations to obtain an overall standard deviation of \(\sqrt{4.95^2 + 5.2^2} \approx 7.18\) . We can now obtain our test statistic \(17/7.18 \approx 2.37\) .

The test statistic can be calibrated against a standard normal reference distribution. The probability of observing a standard normal value that is greater in magnitude than 2.37 is 0.018 (this can be obtained from a computer). This is the p-value, and since it is smaller than the conventional threshold of 0.05, we can claim that there is a statistically significant difference between the average number of charge cycles for the two types of batteries, with the A batteries having more charge cycles on average.

The analysis illustrated here is called a two independent samples Z-test , or just a two sample Z-test . It may be the most commonly employed of all statistical tests. It is also common to see the very similar two sample t-test , which is different only in that it uses the Student t distribution rather than the normal (Gaussian) distribution to calculate the p-values. In fact, there are quite a few minor variations on this testing framework, including “one sided” and “two sided” tests, and tests based on different ways of pooling the variance. Due to the CLT, if the sample size is modestly large (which is the case here), the results of all of these tests will be almost identical. For simplicity, we only cover the Z-test in this course.

Assessment of a correlation #

The tests for comparing proportions and means presented above are quite similar in many ways. To provide one more example of a hypothesis test that is somewhat different, we consider a test for a correlation coefficient.

Recall that the sample correlation coefficient \(\hat{r}\) is used to assess the relationship, or association, between two quantities X and Y that are measured on the same units. For example, we may ask whether two biomarkers, serum creatinine and D-dimer, are correlated with each other. These biomarkers are both commonly used in medical settings and are obtained using blood tests. D-dimer is used to assess whether a person has blood clots, and serum creatinine is used to measure kidney performance.

Suppose we are interested in whether there is a correlation in the population between D-dimer and serum creatinine. The population correlation coefficient between these two quantitites can be denoted \(r\) . Our null hypothesis is \(r=0\) . Suppose that we observe a sample correlation coefficient of \(\hat{r}=0.15\) , using an independent and identically distributed sample of pairs \((x, y)\) , where \(x\) is a D-dimer measurement and \(y\) is a serum creatinine measurement. Are these data consistent with the null hypothesis?

As above, we proceed by constructing a test statistic by taking the estimated statistic and dividing it by its standard error. The approximate standard error for \(\hat{r}\) is \(1/\sqrt{n}\) , where \(n\) is the sample size. The test statistic is therefore \(\sqrt{n}\cdot \hat{r} \approx 1.48\) .

We now calibrate this test statistic by comparing it to a standard normal reference distribution. Recall from the empirical rule that 5% of the time, a standard normal value falls outside the interval (-2, 2). Therefore, if the test statistic is smaller than 2 in magnitude, as is the case here, its p-value is greater than 0.05. Thus, in this case we know that the p-value will exceed 0.05 without calculating it, and therefore there is no basis for claiming that D-dimer and serum creatinine levels are correlated in this population.

Sampling properties of p-values #

A p-value is the most common way of calibrating evidence. Smaller p-values indicate stronger evidence against a null hypothesis. By convention, if the p-value is smaller than some threshold, usually 0.05, we reject the null hypothesis and declare a finding to be “statistically significant”. How can we understand more deeply what this means? One major concern should be obtaining a small p-value when the null hypothesis is true. If the null hypothesis is true, then it is incorrect to reject it. If we reject the null hypothesis, we are making a false claim. This can never be prevented with complete certainty, but we would like to have a very clear understanding of how likely it is to reject the null hypothesis when the null hypothesis is in fact true.

P-values have a special property that when the null distribution is true, the probability of observing a p-value smaller than 0.05 is 0.05 (5%). In fact, the probability of observing a p-value smaller than \(t\) is equal to \(t\) , for any threshold \(t\) . For example, the probability of observing a p-value smaller than 0.1, when the null hypothesis is true, is 10%.

This fact gives a more concrete understanding of how strong the evidence is for a particular p-value. If we always reject the null hypothesis when the p-value is 0.1 or smaller, then over the long run we will reject the null hypothesis 10% of the time when the null hypothesis is true. If we always reject the null hypothesis when the p-value is 0.05 or smaller, then over the long run we will reject the null hypothesis 5% of the time when the null hypothesis is true.

The approach to hypothesis testing discussed above largely follows the framework developed by RA Fisher around 1925. Note that although we mentioned the alternative hypothesis above, we never actually used it. A more elaborate approach to hypothesis testing was developed somewhat later by Egon Pearson and Jerzy Neyman. The “Neyman-Pearson” approach to hypothesis testing is even more formal than Fisher’s approach, and is most suited to highly planned research efforts in which the study is carefully designed, then executed. While ideally all research projects should be carried out this way, in reality we often conduct research using data that are already available, rather than using data that are specifically collected to address the research question.

Neyman-Pearson hypothesis testing involves specifying an alternative hypothesis that we anticipate encountering. Usually this alternative hypothesis represents a realistic guess about what we might find once the data are collected. In each of the three examples above, imagine that the data are not yet collected, and we are asked to specify an alternative hypothesis. We may arrive at the following:

In comparing risk perceptions for COVID, we may anticipate that older people will perceive a 30% risk of dying, and younger people will anticipate a 5% risk of dying.

In comparing the number of charge cycles for two types of batteries, we may anticipate that batter type A will have on average 500 charge cycles, and battery type B will have on average 400 charge cycles.

In assessing the correlation between D-dimer and serum creatinine levels, we may anticipate a correlation of 0.3.

Note that none of the numbers stated here are data-driven – they are specified before any data are collected, so they do not match the results from the data, which were collected only later. These alternative hypotheses are all essentially speculations, based perhaps on related data or theoretical considerations.

There are several benefits of specifying an explicit alternative hypothesis, as done here, even though it is not strictly necessary and can be avoided entirely by adopting Fisher’s approach to hypothesis testing. One benefit of specifying an alternative hypothesis is that we can use it to assess the power of our planned study, which can in turn inform the design of the study, in particular the sample size. The power is the probability of rejecting the null hypothesis when the alternative hypothesis is true. That is, it is the probability of discovering something real. The power should be contrasted with the level of a hypothesis test, which is the probability of rejecting the null hypothesis when the null hypothesis is true. That is, the level is the probability of “discovering” something that is not real.

To calculate the power, recall that for many of the test statistics that we are considering here, the test statistic has the form \(\hat{\theta}/{\rm SE}(\hat{\theta})\) , where \(\hat{\theta}\) is an estimate. For example, \(\hat{\theta}\) ) may be the correlation coefficient between D-dimer and serum creatinine levels. As stated above, the power is the probability of rejecting the null hypothesis when the alternative hypothesis is true. Suppose we decide to reject the null hypothesis when the test statistic is greater than 2, which is approximately equivalent to rejecting the null hypothesis when the p-value is less than 0.05. The following calculation tells us how to obtain the power in this setting:

Under the alternative hypothesis, \(\sqrt{n}(\hat{r} - r)\) approximately follows a standard normal distribution. Therefore, if \(r\) and \(n\) are given, we can easily use the computer to obtain the probability of observing a value greater than \(2 - \sqrt{n}r\) . This gives us the power of the test. For example, if we anticipate \(r=0.3\) and plan to collect data for \(n=100\) observations, the power is 0.84. This is generally considered to be good power – if the true value of \(r\) is in fact 0.3, we would reject the null hypothesis 84% of the time.

A study usually has poor power because it has too small of a sample size. Poorly powered studies can be very misleading, but since large sample sizes are expensive to collect, a lot of research is conducted using sample sizes that yield moderate or even low power. If a study has low power, it is unlikely to reject the null hypothesis even when the alternative hypothesis is true, but it remains possible to reject the null hypothesis when the null hypothesis is true (usually this probability is 5%). Therefore the most likely outcome of a poorly powered study may be an incorrectly rejected null hypothesis.

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A Comprehensive Guide to Hypotheses Tests in Statistics

You will learn the essentials of hypothesis tests, from fundamental concepts to practical applications in statistics.

• Null and alternative hypotheses guide hypothesis tests.
• Significance level and p-value aid decision-making.
• Parametric tests assume specific probability distributions.
• Non-parametric tests offer flexible assumptions.
• Confidence intervals provide estimate precision.  

Introduction to Hypotheses Tests

Hypothesis testing is a statistical tool used to make decisions based on data.

It involves making assumptions about a population parameter and testing its validity using a population sample.

Hypothesis tests help us draw conclusions and make informed decisions in various fields like business, research, and science.

Null and Alternative Hypotheses

The null hypothesis (H0) is an initial claim about a population parameter, typically representing no effect or no difference.

The alternative hypothesis (H1) opposes the null hypothesis, suggesting an effect or difference.

Hypothesis tests aim to determine if there is evidence for the null hypothesis rejection in favor of the alternative hypothesis.

Significance Levels and P-values

The significance level (α), often set at 0.05 or 5%, serves as a threshold for determining if we should reject the null hypothesis.

A p-value, calculated during hypothesis testing, represents the probability of observing the test statistic if the null hypothesis is true.

Suppose the p-value is less than the significance level. We reject the null hypothesis, in that case, indicating that the alternative hypothesis is more likely.

Parametric and Non-Parametric Tests

Parametric tests assume the data follows a specific probability distribution, usually the normal distribution. Examples include the Student’s t-test.

Non-parametric tests do not require such assumptions and are helpful when dealing with data that do not meet the assumptions of parametric tests. Examples include the Mann-Whitney U test.

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Commonly Used Hypotheses Tests

Independent samples t-test:  This analysis compares the means of two independent groups.

Paired samples t-test:  Compares the means of two related groups (e.g., before and after treatment).

Chi-squared test:  Determines if there is a significant association, in a contingency table, between two categorical variables.

Analysis of Variance (ANOVA):  Compares the means of three or more independent groups to determine whether significant differences exist.

Pearson’s Correlation Coefficient (Pearson’s r):  Quantifies the strength and direction of a linear association between two continuous variables.

Simple Linear Regression:  Evaluate whether a significant linear relationship exists between a predictor variable (X) and a continuous outcome variable (y).

Logistic Regression:  Determines the relationship between one or more predictor variables (continuous or categorical) and a binary outcome variable (e.g., success or failure).

Levene’s Test:  Tests the equality of variances between two or more groups, often used as an assumption checks for ANOVA.

Shapiro-Wilk Test:  Assesses the null hypothesis that a data sample is drawn from a population with a normal distribution.

Interpreting the Results of Hypotheses Tests

To interpret the hypothesis test results, compare the p-value to the chosen significance level.

If the p-value falls below the significance level, reject the null hypothesis and infer that a notable effect or difference exists.

Otherwise, fail to reject the null hypothesis, meaning there is insufficient evidence to support the alternative hypothesis.

Other Relevant Information

In addition to understanding the basics of hypothesis tests, it’s crucial to consider other relevant information when interpreting the results.

For example, factors such as effect size, statistical power, and confidence intervals can provide valuable insights and help you make more informed decisions.

Effect size

The effect size represents a quantitative measurement of the strength or magnitude of the observed relationship or effect between variables. It aids in evaluating the practical significance of the results. A statistically significant outcome may not necessarily imply practical relevance. At the same time, a substantial effect size can suggest meaningful findings, even when statistical significance appears marginal.

Statistical power

The power of a test represents the likelihood of accurately rejecting the null hypothesis when it is incorrect. In other words, it’s the likelihood that the test will detect an effect when it exists. Factors affecting the power of a test include the sample size, effect size, and significance level. Enhanced power reduces the likelihood of making an error of Type II — failing to reject the null hypothesis when it ought to be rejected.

Confidence intervals

A confidence interval represents a range where the true population parameter is expected to be found with a specified confidence level (e.g., 95%). Confidence intervals provide additional context to hypothesis testing, helping to assess the estimate’s precision and offering a better understanding of the uncertainty surrounding the results.

By considering these additional aspects when interpreting the results of hypothesis tests, you can gain a more comprehensive understanding of the data and make more informed conclusions.

Hypothesis testing is an indispensable statistical tool for drawing meaningful inferences and making informed data-based decisions.

By comprehending the essential concepts such as null and alternative hypotheses, significance levels, p-values, and the distinction between parametric and non-parametric tests, you can proficiently apply hypothesis testing to a wide range of real-world situations.

Additionally, understanding the importance of effect sizes, statistical power, and confidence intervals will enhance your ability to interpret the results and make better decisions.

With many applications across various fields, including medicine, psychology, business, and environmental sciences, hypothesis testing is a versatile and valuable method for research and data analysis.

A comprehensive grasp of hypothesis testing techniques will enable professionals and researchers to strengthen their decision-making processes, optimize strategies, and deepen their understanding of the relationships between variables, leading to more impactful results and discoveries.

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• > Machine Learning
• > Statistics

What is Hypothesis Testing? Types and Methods

• Soumyaa Rawat
• Jul 23, 2021

Hypothesis Testing  

Hypothesis testing is the act of testing a hypothesis or a supposition in relation to a statistical parameter. Analysts implement hypothesis testing in order to test if a hypothesis is plausible or not. 

In data science and statistics , hypothesis testing is an important step as it involves the verification of an assumption that could help develop a statistical parameter. For instance, a researcher establishes a hypothesis assuming that the average of all odd numbers is an even number. 

In order to find the plausibility of this hypothesis, the researcher will have to test the hypothesis using hypothesis testing methods. Unlike a hypothesis that is ‘supposed’ to stand true on the basis of little or no evidence, hypothesis testing is required to have plausible evidence in order to establish that a statistical hypothesis is true. 

Perhaps this is where statistics play an important role. A number of components are involved in this process. But before understanding the process involved in hypothesis testing in research methodology, we shall first understand the types of hypotheses that are involved in the process. Let us get started! 

Types of Hypotheses

In data sampling, different types of hypothesis are involved in finding whether the tested samples test positive for a hypothesis or not. In this segment, we shall discover the different types of hypotheses and understand the role they play in hypothesis testing.

Alternative Hypothesis

Alternative Hypothesis (H1) or the research hypothesis states that there is a relationship between two variables (where one variable affects the other). The alternative hypothesis is the main driving force for hypothesis testing. 

It implies that the two variables are related to each other and the relationship that exists between them is not due to chance or coincidence. 

When the process of hypothesis testing is carried out, the alternative hypothesis is the main subject of the testing process. The analyst intends to test the alternative hypothesis and verifies its plausibility.

Null Hypothesis

The Null Hypothesis (H0) aims to nullify the alternative hypothesis by implying that there exists no relation between two variables in statistics. It states that the effect of one variable on the other is solely due to chance and no empirical cause lies behind it. 

The null hypothesis is established alongside the alternative hypothesis and is recognized as important as the latter. In hypothesis testing, the null hypothesis has a major role to play as it influences the testing against the alternative hypothesis. 

(Must read: What is ANOVA test? )

Non-Directional Hypothesis

The Non-directional hypothesis states that the relation between two variables has no direction. 

Simply put, it asserts that there exists a relation between two variables, but does not recognize the direction of effect, whether variable A affects variable B or vice versa. 

Directional Hypothesis

The Directional hypothesis, on the other hand, asserts the direction of effect of the relationship that exists between two variables. 

Herein, the hypothesis clearly states that variable A affects variable B, or vice versa. 

Statistical Hypothesis

A statistical hypothesis is a hypothesis that can be verified to be plausible on the basis of statistics. 

By using data sampling and statistical knowledge, one can determine the plausibility of a statistical hypothesis and find out if it stands true or not. 

(Related blog: z-test vs t-test )

Performing Hypothesis Testing  

Now that we have understood the types of hypotheses and the role they play in hypothesis testing, let us now move on to understand the process in a better manner. 

In hypothesis testing, a researcher is first required to establish two hypotheses - alternative hypothesis and null hypothesis in order to begin with the procedure. 

To establish these two hypotheses, one is required to study data samples, find a plausible pattern among the samples, and pen down a statistical hypothesis that they wish to test. 

A random population of samples can be drawn, to begin with hypothesis testing. Among the two hypotheses, alternative and null, only one can be verified to be true. Perhaps the presence of both hypotheses is required to make the process successful. 

At the end of the hypothesis testing procedure, either of the hypotheses will be rejected and the other one will be supported. Even though one of the two hypotheses turns out to be true, no hypothesis can ever be verified 100%. 

(Read also: Types of data sampling techniques )

Therefore, a hypothesis can only be supported based on the statistical samples and verified data. Here is a step-by-step guide for hypothesis testing.

Establish the hypotheses

First things first, one is required to establish two hypotheses - alternative and null, that will set the foundation for hypothesis testing. 

These hypotheses initiate the testing process that involves the researcher working on data samples in order to either support the alternative hypothesis or the null hypothesis. 

Generate a testing plan

Once the hypotheses have been formulated, it is now time to generate a testing plan. A testing plan or an analysis plan involves the accumulation of data samples, determining which statistic is to be considered and laying out the sample size. 

All these factors are very important while one is working on hypothesis testing.

Analyze data samples

As soon as a testing plan is ready, it is time to move on to the analysis part. Analysis of data samples involves configuring statistical values of samples, drawing them together, and deriving a pattern out of these samples. 

While analyzing the data samples, a researcher needs to determine a set of things -

Significance Level - The level of significance in hypothesis testing indicates if a statistical result could have significance if the null hypothesis stands to be true.

Testing Method - The testing method involves a type of sampling-distribution and a test statistic that leads to hypothesis testing. There are a number of testing methods that can assist in the analysis of data samples. 

Test statistic - Test statistic is a numerical summary of a data set that can be used to perform hypothesis testing.

P-value - The P-value interpretation is the probability of finding a sample statistic to be as extreme as the test statistic, indicating the plausibility of the null hypothesis. 

Infer the results

The analysis of data samples leads to the inference of results that establishes whether the alternative hypothesis stands true or not. When the P-value is less than the significance level, the null hypothesis is rejected and the alternative hypothesis turns out to be plausible. 

Methods of Hypothesis Testing

As we have already looked into different aspects of hypothesis testing, we shall now look into the different methods of hypothesis testing. All in all, there are 2 most common types of hypothesis testing methods. They are as follows -

Frequentist Hypothesis Testing

The frequentist hypothesis or the traditional approach to hypothesis testing is a hypothesis testing method that aims on making assumptions by considering current data. 

The supposed truths and assumptions are based on the current data and a set of 2 hypotheses are formulated. A very popular subtype of the frequentist approach is the Null Hypothesis Significance Testing (NHST). 

The NHST approach (involving the null and alternative hypothesis) has been one of the most sought-after methods of hypothesis testing in the field of statistics ever since its inception in the mid-1950s. 

Bayesian Hypothesis Testing

A much unconventional and modern method of hypothesis testing, the Bayesian Hypothesis Testing claims to test a particular hypothesis in accordance with the past data samples, known as prior probability, and current data that lead to the plausibility of a hypothesis. 

The result obtained indicates the posterior probability of the hypothesis. In this method, the researcher relies on ‘prior probability and posterior probability’ to conduct hypothesis testing on hand. 

On the basis of this prior probability, the Bayesian approach tests a hypothesis to be true or false. The Bayes factor, a major component of this method, indicates the likelihood ratio among the null hypothesis and the alternative hypothesis. 

The Bayes factor is the indicator of the plausibility of either of the two hypotheses that are established for hypothesis testing.  

(Also read - Introduction to Bayesian Statistics ) 

To conclude, hypothesis testing, a way to verify the plausibility of a supposed assumption can be done through different methods - the Bayesian approach or the Frequentist approach. 

Although the Bayesian approach relies on the prior probability of data samples, the frequentist approach assumes without a probability. A number of elements involved in hypothesis testing are - significance level, p-level, test statistic, and method of hypothesis testing. 

(Also read: Introduction to probability distributions )

A significant way to determine whether a hypothesis stands true or not is to verify the data samples and identify the plausible hypothesis among the null hypothesis and alternative hypothesis. 

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Lesson 10 of 24 By Avijeet Biswal

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In today’s data-driven world, decisions are based on data all the time. Hypothesis plays a crucial role in that process, whether it may be making business decisions, in the health sector, academia, or in quality improvement. Without hypothesis and hypothesis tests, you risk drawing the wrong conclusions and making bad decisions. In this tutorial, you will look at Hypothesis Testing in Statistics.

What Is Hypothesis Testing in Statistics?

Hypothesis Testing is a type of statistical analysis in which you put your assumptions about a population parameter to the test. It is used to estimate the relationship between 2 statistical variables.

Let's discuss few examples of statistical hypothesis from real-life - 

• A teacher assumes that 60% of his college's students come from lower-middle-class families.
• A doctor believes that 3D (Diet, Dose, and Discipline) is 90% effective for diabetic patients.

Now that you know about hypothesis testing, look at the two types of hypothesis testing in statistics.

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Importance of Hypothesis Testing in Data Analysis

Here is what makes hypothesis testing so important in data analysis and why it is key to making better decisions:

Avoiding Misleading Conclusions (Type I and Type II Errors)

One of the biggest benefits of hypothesis testing is that it helps you avoid jumping to the wrong conclusions. For instance, a Type I error could occur if a company launches a new product thinking it will be a hit, only to find out later that the data misled them. A Type II error might happen when a company overlooks a potentially successful product because their testing wasn’t thorough enough. By setting up the right significance level and carefully calculating the p-value, hypothesis testing minimizes the chances of these errors, leading to more accurate results.

Making Smarter Choices

Hypothesis testing is key to making smarter, evidence-based decisions. Let’s say a city planner wants to determine if building a new park will increase community engagement. By testing the hypothesis using data from similar projects, they can make an informed choice. Similarly, a teacher might use hypothesis testing to see if a new teaching method actually improves student performance. It’s about taking the guesswork out of decisions and relying on solid evidence instead.

Optimizing Business Tactics

In business, hypothesis testing is invaluable for testing new ideas and strategies before fully committing to them. For example, an e-commerce company might want to test whether offering free shipping increases sales. By using hypothesis testing, they can compare sales data from customers who received free shipping offers and those who didn’t. This allows them to base their business decisions on data, not hunches, reducing the risk of costly mistakes.

Hypothesis Testing Formula

Z = ( x̅ – μ0 ) / (σ /√n)

• Here, x̅ is the sample mean,
• μ0 is the population mean,
• σ is the standard deviation,
• n is the sample size.

How Hypothesis Testing Works?

An analyst performs hypothesis testing on a statistical sample to present evidence of the plausibility of the null hypothesis. Measurements and analyses are conducted on a random sample of the population to test a theory. Analysts use a random population sample to test two hypotheses: the null and alternative hypotheses.

The null hypothesis is typically an equality hypothesis between population parameters; for example, a null hypothesis may claim that the population means return equals zero. The alternate hypothesis is essentially the inverse of the null hypothesis (e.g., the population means the return is not equal to zero). As a result, they are mutually exclusive, and only one can be correct. One of the two possibilities, however, will always be correct.

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Null Hypothesis and Alternative Hypothesis

The Null Hypothesis is the assumption that the event will not occur. A null hypothesis has no bearing on the study's outcome unless it is rejected.

H0 is the symbol for it, and it is pronounced H-naught.

The Alternate Hypothesis is the logical opposite of the null hypothesis. The acceptance of the alternative hypothesis follows the rejection of the null hypothesis. H1 is the symbol for it.

Let's understand this with an example.

A sanitizer manufacturer claims that its product kills 95 percent of germs on average. 

To put this company's claim to the test, create a null and alternate hypothesis.

H0 (Null Hypothesis): Average = 95%.

Alternative Hypothesis (H1): The average is less than 95%.

Another straightforward example to understand this concept is determining whether or not a coin is fair and balanced. The null hypothesis states that the probability of a show of heads is equal to the likelihood of a show of tails. In contrast, the alternate theory states that the probability of a show of heads and tails would be very different.

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Hypothesis Testing Calculation With Examples

Let's consider a hypothesis test for the average height of women in the United States. Suppose our null hypothesis is that the average height is 5'4". We gather a sample of 100 women and determine their average height is 5'5". The standard deviation of population is 2.

To calculate the z-score, we would use the following formula:

z = ( x̅ – μ0 ) / (σ /√n)

z = (5'5" - 5'4") / (2" / √100)

z = 0.5 / (0.045)

We will reject the null hypothesis as the z-score of 11.11 is very large and conclude that there is evidence to suggest that the average height of women in the US is greater than 5'4".

Steps in Hypothesis Testing

Hypothesis testing is a statistical method to determine if there is enough evidence in a sample of data to infer that a certain condition is true for the entire population. Here’s a breakdown of the typical steps involved in hypothesis testing:

Formulate Hypotheses

• Null Hypothesis (H0): This hypothesis states that there is no effect or difference, and it is the hypothesis you attempt to reject with your test.
• Alternative Hypothesis (H1 or Ha): This hypothesis is what you might believe to be true or hope to prove true. It is usually considered the opposite of the null hypothesis.

Choose the Significance Level (α)

The significance level, often denoted by alpha (α), is the probability of rejecting the null hypothesis when it is true. Common choices for α are 0.05 (5%), 0.01 (1%), and 0.10 (10%).

Select the Appropriate Test

Choose a statistical test based on the type of data and the hypothesis. Common tests include t-tests, chi-square tests, ANOVA, and regression analysis. The selection depends on data type, distribution, sample size, and whether the hypothesis is one-tailed or two-tailed.

Collect Data

Gather the data that will be analyzed in the test. To infer conclusions accurately, this data should be representative of the population.

Calculate the Test Statistic

Based on the collected data and the chosen test, calculate a test statistic that reflects how much the observed data deviates from the null hypothesis.

Determine the p-value

The p-value is the probability of observing test results at least as extreme as the results observed, assuming the null hypothesis is correct. It helps determine the strength of the evidence against the null hypothesis.

Make a Decision

Compare the p-value to the chosen significance level:

• If the p-value ≤ α: Reject the null hypothesis, suggesting sufficient evidence in the data supports the alternative hypothesis.
• If the p-value > α: Do not reject the null hypothesis, suggesting insufficient evidence to support the alternative hypothesis.

Report the Results

Present the findings from the hypothesis test, including the test statistic, p-value, and the conclusion about the hypotheses.

Perform Post-hoc Analysis (if necessary)

Depending on the results and the study design, further analysis may be needed to explore the data more deeply or to address multiple comparisons if several hypotheses were tested simultaneously.

Types of Hypothesis Testing

To determine whether a discovery or relationship is statistically significant, hypothesis testing uses a z-test. It usually checks to see if two means are the same (the null hypothesis). Only when the population standard deviation is known and the sample size is 30 data points or more, can a z-test be applied.

A statistical test called a t-test is employed to compare the means of two groups. To determine whether two groups differ or if a procedure or treatment affects the population of interest, it is frequently used in hypothesis testing.

3. Chi-Square 

You utilize a Chi-square test for hypothesis testing concerning whether your data is as predicted. To determine if the expected and observed results are well-fitted, the Chi-square test analyzes the differences between categorical variables from a random sample. The test's fundamental premise is that the observed values in your data should be compared to the predicted values that would be present if the null hypothesis were true.

ANOVA , or Analysis of Variance, is a statistical method used to compare the means of three or more groups. It’s particularly useful when you want to see if there are significant differences between multiple groups. For instance, in business, a company might use ANOVA to analyze whether three different stores are performing differently in terms of sales. It’s also widely used in fields like medical research and social sciences, where comparing group differences can provide valuable insights.

Hypothesis Testing and Confidence Intervals

Both confidence intervals and hypothesis tests are inferential techniques that depend on approximating the sample distribution. Data from a sample is used to estimate a population parameter using confidence intervals. Data from a sample is used in hypothesis testing to examine a given hypothesis. We must have a postulated parameter to conduct hypothesis testing.

Bootstrap distributions and randomization distributions are created using comparable simulation techniques. The observed sample statistic is the focal point of a bootstrap distribution, whereas the null hypothesis value is the focal point of a randomization distribution.

A variety of feasible population parameter estimates are included in confidence ranges. In this lesson, we created just two-tailed confidence intervals. There is a direct connection between these two-tail confidence intervals and these two-tail hypothesis tests. The results of a two-tailed hypothesis test and two-tailed confidence intervals typically provide the same results. In other words, a hypothesis test at the 0.05 level will virtually always fail to reject the null hypothesis if the 95% confidence interval contains the predicted value. A hypothesis test at the 0.05 level will nearly certainly reject the null hypothesis if the 95% confidence interval does not include the hypothesized parameter.

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Simple and Composite Hypothesis Testing

Depending on the population distribution, you can classify the statistical hypothesis into two types.

Simple Hypothesis: A simple hypothesis specifies an exact value for the parameter.

Composite Hypothesis: A composite hypothesis specifies a range of values.

A company is claiming that their average sales for this quarter are 1000 units. This is an example of a simple hypothesis.

Suppose the company claims that the sales are in the range of 900 to 1000 units. Then this is a case of a composite hypothesis.

One-Tailed and Two-Tailed Hypothesis Testing

The One-Tailed test, also called a directional test, considers a critical region of data that would result in the null hypothesis being rejected if the test sample falls into it, inevitably meaning the acceptance of the alternate hypothesis.

In a one-tailed test, the critical distribution area is one-sided, meaning the test sample is either greater or lesser than a specific value.

In two tails, the test sample is checked to be greater or less than a range of values in a Two-Tailed test, implying that the critical distribution area is two-sided.

If the sample falls within this range, the alternate hypothesis will be accepted, and the null hypothesis will be rejected.

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Right Tailed Hypothesis Testing

If the larger than (>) sign appears in your hypothesis statement, you are using a right-tailed test, also known as an upper test. Or, to put it another way, the disparity is to the right. For instance, you can contrast the battery life before and after a change in production. Your hypothesis statements can be the following if you want to know if the battery life is longer than the original (let's say 90 hours):

• The null hypothesis is (H0 <= 90) or less change.
• A possibility is that battery life has risen (H1) > 90.

The crucial point in this situation is that the alternate hypothesis (H1), not the null hypothesis, decides whether you get a right-tailed test.

Left Tailed Hypothesis Testing

Alternative hypotheses that assert the true value of a parameter is lower than the null hypothesis are tested with a left-tailed test; they are indicated by the asterisk "<".

Suppose H0: mean = 50 and H1: mean not equal to 50

According to the H1, the mean can be greater than or less than 50. This is an example of a Two-tailed test.

In a similar manner, if H0: mean >=50, then H1: mean <50

Here the mean is less than 50. It is called a One-tailed test.

Type 1 and Type 2 Error

A hypothesis test can result in two types of errors.

Type 1 Error: A Type-I error occurs when sample results reject the null hypothesis despite being true.

Type 2 Error: A Type-II error occurs when the null hypothesis is not rejected when it is false, unlike a Type-I error.

Suppose a teacher evaluates the examination paper to decide whether a student passes or fails.

H0: Student has passed

H1: Student has failed

Type I error will be the teacher failing the student [rejects H0] although the student scored the passing marks [H0 was true]. 

Type II error will be the case where the teacher passes the student [do not reject H0] although the student did not score the passing marks [H1 is true].

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Practice Problems on Hypothesis Testing

Here are the practice problems on hypothesis testing that will help you understand how to apply these concepts in real-world scenarios:

A telecom service provider claims that customers spend an average of ₹400 per month, with a standard deviation of ₹25. However, a random sample of 50 customer bills shows a mean of ₹250 and a standard deviation of ₹15. Does this sample data support the service provider’s claim?

Solution: Let’s break this down:

• Null Hypothesis (H0): The average amount spent per month is ₹400.
• Alternate Hypothesis (H1): The average amount spent per month is not ₹400.
• Population Standard Deviation (σ): ₹25
• Sample Size (n): 50
• Sample Mean (x̄): ₹250

1. Calculate the z-value:

z=250-40025/50 −42.42

2. Compare with critical z-values: For a 5% significance level, critical z-values are -1.96 and +1.96. Since -42.42 is far outside this range, we reject the null hypothesis. The sample data suggests that the average amount spent is significantly different from ₹400.

Out of 850 customers, 400 made online grocery purchases. Can we conclude that more than 50% of customers are moving towards online grocery shopping?

Solution: Here’s how to approach it:

• Proportion of customers who shopped online (p): 400 / 850 = 0.47
• Null Hypothesis (H0): The proportion of online shoppers is 50% or more.
• Alternate Hypothesis (H1): The proportion of online shoppers is less than 50%.
• Sample Size (n): 850
• Significance Level (α): 5%

z=p-PP(1-P)/n

z=0.47-0.500.50.5/850  −1.74

2. Compare with the critical z-value: For a 5% significance level (one-tailed test), the critical z-value is -1.645. Since -1.74 is less than -1.645, we reject the null hypothesis. This means the data does not support the idea that most customers are moving towards online grocery shopping.

In a study of code quality, Team A has 250 errors in 1000 lines of code, and Team B has 300 errors in 800 lines of code. Can we say Team B performs worse than Team A?

Solution: Let’s analyze it:

• Proportion of errors for Team A (pA): 250 / 1000 = 0.25
• Proportion of errors for Team B (pB): 300 / 800 = 0.375
• Null Hypothesis (H0): Team B’s error rate is less than or equal to Team A’s.
• Alternate Hypothesis (H1): Team B’s error rate is greater than Team A’s.
• Sample Size for Team A (nA): 1000
• Sample Size for Team B (nB): 800

p=nApA+nBpBnA+nB

p=10000.25+8000.3751000+800 ≈ 0.305

z=​pA−pB​p(1-p)(1nA+1nB)

z=​0.25−0.375​0.305(1-0.305) (11000+1800) ≈ −5.72

2. Compare with the critical z-value: For a 5% significance level (one-tailed test), the critical z-value is +1.645. Since -5.72 is far less than +1.645, we reject the null hypothesis. The data indicates that Team B’s performance is significantly worse than Team A’s.

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Applications of Hypothesis Testing

Apart from the practical problems, let's look at the real-world applications of hypothesis testing across various fields:

Medicine and Healthcare

In medicine, hypothesis testing plays a pivotal role in assessing the success of new treatments. For example, researchers may want to find out if a new exercise regimen improves heart health. By comparing data from patients who followed the program to those who didn’t, they can determine if the exercise significantly improves health outcomes. Such rigorous testing allows medical professionals to rely on proven methods rather than assumptions.

Quality Control and Manufacturing

In manufacturing, ensuring product quality is vital, and hypothesis testing helps maintain those standards. Suppose a beverage company introduces a new bottling process and wants to verify if it reduces contamination. By analyzing samples from the new and old processes, hypothesis testing can reveal whether the new method reduces the risk of contamination. This allows manufacturers to implement improvements that enhance product safety and quality confidently.

Education and Learning

In education and learning, hypothesis testing is a tool to evaluate the impact of innovative teaching techniques. Imagine a situation where teachers introduce project-based learning to boost critical thinking skills. By comparing the performance of students who engaged in project-based learning with those in traditional settings, educators can test their hypothesis. The results can help educators make informed choices about adopting new teaching strategies.

Environmental Science

Hypothesis testing is essential in environmental science for evaluating the effectiveness of conservation measures. For example, scientists might explore whether a new water management strategy improves river health. By collecting and comparing data on water quality before and after the implementation of the strategy, they can determine whether the intervention leads to positive changes. Such findings are crucial for guiding environmental decisions that have long-term impacts.

Marketing and Advertising

In marketing, businesses use hypothesis testing to refine their approaches. For instance, a clothing brand might test if offering limited-time discounts increases customer loyalty. By running campaigns with and without the discount and analyzing the outcomes, they can assess if the strategy boosts customer retention. Data-driven insights from hypothesis testing enable companies to design marketing strategies that resonate with their audience and drive growth.

Limitations of Hypothesis Testing

Hypothesis testing has some limitations that researchers should be aware of:

• It cannot prove or establish the truth: Hypothesis testing provides evidence to support or reject a hypothesis, but it cannot confirm the absolute truth of the research question.
• Results are sample-specific: Hypothesis testing is based on analyzing a sample from a population, and the conclusions drawn are specific to that particular sample.
• Possible errors: During hypothesis testing, there is a chance of committing type I error (rejecting a true null hypothesis) or type II error (failing to reject a false null hypothesis).
• Assumptions and requirements: Different tests have specific assumptions and requirements that must be met to accurately interpret results.

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After reading this tutorial, you would have a much better understanding of hypothesis testing, one of the most important concepts in the field of Data Science . The majority of hypotheses are based on speculation about observed behavior, natural phenomena, or established theories.

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1. What is hypothesis testing in statistics with example?

Hypothesis testing is a statistical method used to determine if there is enough evidence in a sample data to draw conclusions about a population. It involves formulating two competing hypotheses, the null hypothesis (H0) and the alternative hypothesis (Ha), and then collecting data to assess the evidence. An example: testing if a new drug improves patient recovery (Ha) compared to the standard treatment (H0) based on collected patient data.

2. What is H0 and H1 in statistics?

In statistics, H0​ and H1​ represent the null and alternative hypotheses. The null hypothesis, H0​, is the default assumption that no effect or difference exists between groups or conditions. The alternative hypothesis, H1​, is the competing claim suggesting an effect or a difference. Statistical tests determine whether to reject the null hypothesis in favor of the alternative hypothesis based on the data.

3. What is a simple hypothesis with an example?

A simple hypothesis is a specific statement predicting a single relationship between two variables. It posits a direct and uncomplicated outcome. For example, a simple hypothesis might state, "Increased sunlight exposure increases the growth rate of sunflowers." Here, the hypothesis suggests a direct relationship between the amount of sunlight (independent variable) and the growth rate of sunflowers (dependent variable), with no additional variables considered.

4. What are the 3 major types of hypothesis?

The three major types of hypotheses are:

• Null Hypothesis (H0): Represents the default assumption, stating that there is no significant effect or relationship in the data.
• Alternative Hypothesis (Ha): Contradicts the null hypothesis and proposes a specific effect or relationship that researchers want to investigate.
• Nondirectional Hypothesis: An alternative hypothesis that doesn't specify the direction of the effect, leaving it open for both positive and negative possibilities.

5. What software tools can assist with hypothesis testing?

Several software tools offering distinct features can help with hypothesis testing. R and RStudio are popular for their advanced statistical capabilities. The Python ecosystem, including libraries like SciPy and Statsmodels, also supports hypothesis testing. SAS and SPSS are well-established tools for comprehensive statistical analysis. For basic testing, Excel offers simple built-in functions.

6. How do I interpret the results of a hypothesis test?

Interpreting hypothesis test results involves comparing the p-value to the significance level (alpha). If the p-value is less than or equal to alpha, you can reject the null hypothesis, indicating statistical significance. This suggests that the observed effect is unlikely to have occurred by chance, validating your analysis findings.

7. Why is sample size important in hypothesis testing?

Sample size is crucial in hypothesis testing as it affects the test’s power. A larger sample size increases the likelihood of detecting a true effect, reducing the risk of Type II errors. Conversely, a small sample may lack the statistical power needed to identify differences, potentially leading to inaccurate conclusions.

8. Can hypothesis testing be used for non-numerical data?

Yes, hypothesis testing can be applied to non-numerical data through non-parametric tests. These tests are ideal when data doesn't meet parametric assumptions or when dealing with categorical data. Non-parametric tests, like the Chi-square or Mann-Whitney U test, provide robust methods for analyzing non-numerical data and drawing meaningful conclusions.

9. How do I choose the proper hypothesis test?

Selecting the right hypothesis test depends on several factors: the objective of your analysis, the type of data (numerical or categorical), and the sample size. Consider whether you're comparing means, proportions, or associations, and whether your data follows a normal distribution. The correct choice ensures accurate results tailored to your research question.

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About the Author

Avijeet is a Senior Research Analyst at Simplilearn. Passionate about Data Analytics, Machine Learning, and Deep Learning, Avijeet is also interested in politics, cricket, and football.

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Hypothesis Testing

About hypothesis testing.

Watch the video for a brief overview of hypothesis testing:

Can’t see the video? Click here to watch it on YouTube.

Contents (Click to skip to the section):

What is a Hypothesis?

What is hypothesis testing.

• Hypothesis Testing Examples (One Sample Z Test).
• Hypothesis Test on a Mean (TI 83).

Bayesian Hypothesis Testing.

• More Hypothesis Testing Articles
• Hypothesis Tests in One Picture
• Critical Values

What is the Null Hypothesis?

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A hypothesis is an educated guess about something in the world around you. It should be testable, either by experiment or observation. For example:

• A new medicine you think might work.
• A way of teaching you think might be better.
• A possible location of new species.
• A fairer way to administer standardized tests.

It can really be anything at all as long as you can put it to the test.

What is a Hypothesis Statement?

If you are going to propose a hypothesis, it’s customary to write a statement. Your statement will look like this: “If I…(do this to an independent variable )….then (this will happen to the dependent variable ).” For example:

• If I (decrease the amount of water given to herbs) then (the herbs will increase in size).
• If I (give patients counseling in addition to medication) then (their overall depression scale will decrease).
• If I (give exams at noon instead of 7) then (student test scores will improve).
• If I (look in this certain location) then (I am more likely to find new species).

A good hypothesis statement should:

• Include an “if” and “then” statement (according to the University of California).
• Include both the independent and dependent variables.
• Be testable by experiment, survey or other scientifically sound technique.
• Be based on information in prior research (either yours or someone else’s).
• Have design criteria (for engineering or programming projects).

Hypothesis testing can be one of the most confusing aspects for students, mostly because before you can even perform a test, you have to know what your null hypothesis is. Often, those tricky word problems that you are faced with can be difficult to decipher. But it’s easier than you think; all you need to do is:

• Figure out your null hypothesis,
• State your null hypothesis,
• Choose what kind of test you need to perform,
• Either support or reject the null hypothesis .

If you trace back the history of science, the null hypothesis is always the accepted fact. Simple examples of null hypotheses that are generally accepted as being true are:

• DNA is shaped like a double helix.
• There are 8 planets in the solar system (excluding Pluto).
• Taking Vioxx can increase your risk of heart problems (a drug now taken off the market).

How do I State the Null Hypothesis?

You won’t be required to actually perform a real experiment or survey in elementary statistics (or even disprove a fact like “Pluto is a planet”!), so you’ll be given word problems from real-life situations. You’ll need to figure out what your hypothesis is from the problem. This can be a little trickier than just figuring out what the accepted fact is. With word problems, you are looking to find a fact that is nullifiable (i.e. something you can reject).

Hypothesis Testing Examples #1: Basic Example

A researcher thinks that if knee surgery patients go to physical therapy twice a week (instead of 3 times), their recovery period will be longer. Average recovery times for knee surgery patients is 8.2 weeks.

The hypothesis statement in this question is that the researcher believes the average recovery time is more than 8.2 weeks. It can be written in mathematical terms as: H 1 : μ > 8.2

Next, you’ll need to state the null hypothesis .  That’s what will happen if the researcher is wrong . In the above example, if the researcher is wrong then the recovery time is less than or equal to 8.2 weeks. In math, that’s: H 0 μ ≤ 8.2

Rejecting the null hypothesis

Ten or so years ago, we believed that there were 9 planets in the solar system. Pluto was demoted as a planet in 2006. The null hypothesis of “Pluto is a planet” was replaced by “Pluto is not a planet.” Of course, rejecting the null hypothesis isn’t always that easy— the hard part is usually figuring out what your null hypothesis is in the first place.

Hypothesis Testing Examples (One Sample Z Test)

The one sample z test isn’t used very often (because we rarely know the actual population standard deviation ). However, it’s a good idea to understand how it works as it’s one of the simplest tests you can perform in hypothesis testing. In English class you got to learn the basics (like grammar and spelling) before you could write a story; think of one sample z tests as the foundation for understanding more complex hypothesis testing. This page contains two hypothesis testing examples for one sample z-tests .

One Sample Hypothesis Testing Example: One Tailed Z Test

Watch the video for an example:

A principal at a certain school claims that the students in his school are above average intelligence. A random sample of thirty students IQ scores have a mean score of 112.5. Is there sufficient evidence to support the principal’s claim? The mean population IQ is 100 with a standard deviation of 15.

Step 1: State the Null hypothesis . The accepted fact is that the population mean is 100, so: H 0 : μ = 100.

Step 2: State the Alternate Hypothesis . The claim is that the students have above average IQ scores, so: H 1 : μ > 100. The fact that we are looking for scores “greater than” a certain point means that this is a one-tailed test.

Step 4: State the alpha level . If you aren’t given an alpha level , use 5% (0.05).

Step 5: Find the rejection region area (given by your alpha level above) from the z-table . An area of .05 is equal to a z-score of 1.645.

Step 6: If Step 6 is greater than Step 5, reject the null hypothesis. If it’s less than Step 5, you cannot reject the null hypothesis. In this case, it is more (4.56 > 1.645), so you can reject the null.

One Sample Hypothesis Testing Examples: #3

Watch the video for an example of a two-tailed z-test:

Blood glucose levels for obese patients have a mean of 100 with a standard deviation of 15. A researcher thinks that a diet high in raw cornstarch will have a positive or negative effect on blood glucose levels. A sample of 30 patients who have tried the raw cornstarch diet have a mean glucose level of 140. Test the hypothesis that the raw cornstarch had an effect.

• State the null hypothesis : H 0 :μ=100
• State the alternate hypothesis : H 1 :≠100
• State your alpha level. We’ll use 0.05 for this example. As this is a two-tailed test, split the alpha into two. 0.05/2=0.025
• Find the z-score associated with your alpha level . You’re looking for the area in one tail only . A z-score for 0.75(1-0.025=0.975) is 1.96. As this is a two-tailed test, you would also be considering the left tail (z = 1.96)
•   If Step 5 is less than -1.96 or greater than 1.96 (Step 3), reject the null hypothesis . In this case, it is greater, so you can reject the null.

*This process is made much easier if you use a TI-83 or Excel to calculate the z-score (the “critical value”). See:

• Critical z value TI 83
• Z Score in Excel

Hypothesis Testing Examples: Mean (Using TI 83)

You can use the TI 83 calculator for hypothesis testing, but the calculator won’t figure out the null and alternate hypotheses; that’s up to you to read the question and input it into the calculator.

Example problem : A sample of 200 people has a mean age of 21 with a population standard deviation (σ) of 5. Test the hypothesis that the population mean is 18.9 at α = 0.05.

Step 1: State the null hypothesis. In this case, the null hypothesis is that the population mean is 18.9, so we write: H 0 : μ = 18.9

Step 2: State the alternative hypothesis. We want to know if our sample, which has a mean of 21 instead of 18.9, really is different from the population, therefore our alternate hypothesis: H 1 : μ ≠ 18.9

Step 3: Press Stat then press the right arrow twice to select TESTS.

Step 4: Press 1 to select 1:Z-Test… . Press ENTER.

Step 5: Use the right arrow to select Stats .

Step 6: Enter the data from the problem: μ 0 : 18.9 σ: 5 x : 21 n: 200 μ: ≠μ 0

Step 7: Arrow down to Calculate and press ENTER. The calculator shows the p-value: p = 2.87 × 10 -9

This is smaller than our alpha value of .05. That means we should reject the null hypothesis .

Bayesian Hypothesis Testing: What is it?

Bayesian hypothesis testing helps to answer the question: Can the results from a test or survey be repeated? Why do we care if a test can be repeated? Let’s say twenty people in the same village came down with leukemia. A group of researchers find that cell-phone towers are to blame. However, a second study found that cell-phone towers had nothing to do with the cancer cluster in the village. In fact, they found that the cancers were completely random. If that sounds impossible, it actually can happen! Clusters of cancer can happen simply by chance . There could be many reasons why the first study was faulty. One of the main reasons could be that they just didn’t take into account that sometimes things happen randomly and we just don’t know why.

It’s good science to let people know if your study results are solid, or if they could have happened by chance. The usual way of doing this is to test your results with a p-value . A p value is a number that you get by running a hypothesis test on your data. A P value of 0.05 (5%) or less is usually enough to claim that your results are repeatable. However, there’s another way to test the validity of your results: Bayesian Hypothesis testing. This type of testing gives you another way to test the strength of your results.

Traditional testing (the type you probably came across in elementary stats or AP stats) is called Non-Bayesian. It is how often an outcome happens over repeated runs of the experiment. It’s an objective view of whether an experiment is repeatable. Bayesian hypothesis testing is a subjective view of the same thing. It takes into account how much faith you have in your results. In other words, would you wager money on the outcome of your experiment?

Differences Between Traditional and Bayesian Hypothesis Testing.

Traditional testing (Non Bayesian) requires you to repeat sampling over and over, while Bayesian testing does not. The main different between the two is in the first step of testing: stating a probability model. In Bayesian testing you add prior knowledge to this step. It also requires use of a posterior probability , which is the conditional probability given to a random event after all the evidence is considered.

Arguments for Bayesian Testing.

Many researchers think that it is a better alternative to traditional testing, because it:

• Includes prior knowledge about the data.
• Takes into account personal beliefs about the results.

Arguments against.

• Including prior data or knowledge isn’t justifiable.
• It is difficult to calculate compared to non-Bayesian testing.

Back to top

Hypothesis Testing Articles

• What is Ad Hoc Testing?
• Composite Hypothesis Test
• What is a Rejection Region?
• What is a Two Tailed Test?
• How to Decide if a Hypothesis Test is a One Tailed Test or a Two Tailed Test.
• How to Decide if a Hypothesis is a Left Tailed Test or a Right-Tailed Test.
• How to State the Null Hypothesis in Statistics.
• How to Find a Critical Value .
• How to Support or Reject a Null Hypothesis.

Specific Tests:

• Brunner Munzel Test (Generalized Wilcoxon Test).
• Chi Square Test for Normality.
• Cochran-Mantel-Haenszel Test.
• Granger Causality Test .
• Hotelling’s T-Squared.
• KPSS Test .
• What is a Likelihood-Ratio Test?
• Log rank test .
• MANCOVA Assumptions.
• MANCOVA Sample Size.
• Marascuilo Procedure
• Rao’s Spacing Test
• Rayleigh test of uniformity.
• Sequential Probability Ratio Test.
• How to Run a Sign Test.
• T Test: one sample.
• T-Test: Two sample .
• Welch’s ANOVA .
• Welch’s Test for Unequal Variances .
• Z-Test: one sample .
• Z Test: Two Proportion.
• Wald Test .

Related Articles:

• What is an Acceptance Region?
• How to Calculate Chebyshev’s Theorem.
• Contrast Analysis
• Decision Rule.
• Degrees of Freedom .
• Directional Test
• False Discovery Rate
• How to calculate the Least Significant Difference.
• Levels in Statistics.
• How to Calculate Margin of Error.
• Mean Difference (Difference in Means)
• The Multiple Testing Problem .
• What is the Neyman-Pearson Lemma?
• What is an Omnibus Test?
• One Sample Median Test .
• How to Find a Sample Size (General Instructions).
• Sig 2(Tailed) meaning in results
• What is a Standardized Test Statistic?
• How to Find Standard Error
• Standardized values: Example.
• How to Calculate a T-Score.
• T-Score Vs. a Z.Score.
• Testing a Single Mean.
• Unequal Sample Sizes.
• Uniformly Most Powerful Tests.
• How to Calculate a Z-Score.

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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A Beginner’s Guide to Hypothesis Testing in Business

• 30 Mar 2021

Becoming a more data-driven decision-maker can bring several benefits to your organization, enabling you to identify new opportunities to pursue and threats to abate. Rather than allowing subjective thinking to guide your business strategy, backing your decisions with data can empower your company to become more innovative and, ultimately, profitable.

If you’re new to data-driven decision-making, you might be wondering how data translates into business strategy. The answer lies in generating a hypothesis and verifying or rejecting it based on what various forms of data tell you.

Below is a look at hypothesis testing and the role it plays in helping businesses become more data-driven.

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What Is Hypothesis Testing?

To understand what hypothesis testing is, it’s important first to understand what a hypothesis is.

A hypothesis or hypothesis statement seeks to explain why something has happened, or what might happen, under certain conditions. It can also be used to understand how different variables relate to each other. Hypotheses are often written as if-then statements; for example, “If this happens, then this will happen.”

Hypothesis testing , then, is a statistical means of testing an assumption stated in a hypothesis. While the specific methodology leveraged depends on the nature of the hypothesis and data available, hypothesis testing typically uses sample data to extrapolate insights about a larger population.

Hypothesis Testing in Business

When it comes to data-driven decision-making, there’s a certain amount of risk that can mislead a professional. This could be due to flawed thinking or observations, incomplete or inaccurate data , or the presence of unknown variables. The danger in this is that, if major strategic decisions are made based on flawed insights, it can lead to wasted resources, missed opportunities, and catastrophic outcomes.

The real value of hypothesis testing in business is that it allows professionals to test their theories and assumptions before putting them into action. This essentially allows an organization to verify its analysis is correct before committing resources to implement a broader strategy.

As one example, consider a company that wishes to launch a new marketing campaign to revitalize sales during a slow period. Doing so could be an incredibly expensive endeavor, depending on the campaign’s size and complexity. The company, therefore, may wish to test the campaign on a smaller scale to understand how it will perform.

In this example, the hypothesis that’s being tested would fall along the lines of: “If the company launches a new marketing campaign, then it will translate into an increase in sales.” It may even be possible to quantify how much of a lift in sales the company expects to see from the effort. Pending the results of the pilot campaign, the business would then know whether it makes sense to roll it out more broadly.

Related: 9 Fundamental Data Science Skills for Business Professionals

Key Considerations for Hypothesis Testing

1. alternative hypothesis and null hypothesis.

In hypothesis testing, the hypothesis that’s being tested is known as the alternative hypothesis . Often, it’s expressed as a correlation or statistical relationship between variables. The null hypothesis , on the other hand, is a statement that’s meant to show there’s no statistical relationship between the variables being tested. It’s typically the exact opposite of whatever is stated in the alternative hypothesis.

For example, consider a company’s leadership team that historically and reliably sees $12 million in monthly revenue. They want to understand if reducing the price of their services will attract more customers and, in turn, increase revenue.

In this case, the alternative hypothesis may take the form of a statement such as: “If we reduce the price of our flagship service by five percent, then we’ll see an increase in sales and realize revenues greater than $12 million in the next month.”

The null hypothesis, on the other hand, would indicate that revenues wouldn’t increase from the base of $12 million, or might even decrease.

Check out the video below about the difference between an alternative and a null hypothesis, and subscribe to our YouTube channel for more explainer content.

2. Significance Level and P-Value

Statistically speaking, if you were to run the same scenario 100 times, you’d likely receive somewhat different results each time. If you were to plot these results in a distribution plot, you’d see the most likely outcome is at the tallest point in the graph, with less likely outcomes falling to the right and left of that point.

With this in mind, imagine you’ve completed your hypothesis test and have your results, which indicate there may be a correlation between the variables you were testing. To understand your results' significance, you’ll need to identify a p-value for the test, which helps note how confident you are in the test results.

In statistics, the p-value depicts the probability that, assuming the null hypothesis is correct, you might still observe results that are at least as extreme as the results of your hypothesis test. The smaller the p-value, the more likely the alternative hypothesis is correct, and the greater the significance of your results.

3. One-Sided vs. Two-Sided Testing

When it’s time to test your hypothesis, it’s important to leverage the correct testing method. The two most common hypothesis testing methods are one-sided and two-sided tests , or one-tailed and two-tailed tests, respectively.

Typically, you’d leverage a one-sided test when you have a strong conviction about the direction of change you expect to see due to your hypothesis test. You’d leverage a two-sided test when you’re less confident in the direction of change.

4. Sampling

To perform hypothesis testing in the first place, you need to collect a sample of data to be analyzed. Depending on the question you’re seeking to answer or investigate, you might collect samples through surveys, observational studies, or experiments.

A survey involves asking a series of questions to a random population sample and recording self-reported responses.

Observational studies involve a researcher observing a sample population and collecting data as it occurs naturally, without intervention.

Finally, an experiment involves dividing a sample into multiple groups, one of which acts as the control group. For each non-control group, the variable being studied is manipulated to determine how the data collected differs from that of the control group.

Learn How to Perform Hypothesis Testing

Hypothesis testing is a complex process involving different moving pieces that can allow an organization to effectively leverage its data and inform strategic decisions.

If you’re interested in better understanding hypothesis testing and the role it can play within your organization, one option is to complete a course that focuses on the process. Doing so can lay the statistical and analytical foundation you need to succeed.

Do you want to learn more about hypothesis testing? Explore Business Analytics —one of our online business essentials courses —and download our Beginner’s Guide to Data & Analytics .

About the Author

Hypothesis Testing - Analysis of Variance (ANOVA)

Lisa Sullivan, PhD

Professor of Biostatistics

Boston University School of Public Health

Introduction

This module will continue the discussion of hypothesis testing, where a specific statement or hypothesis is generated about a population parameter, and sample statistics are used to assess the likelihood that the hypothesis is true. The hypothesis is based on available information and the investigator's belief about the population parameters. The specific test considered here is called analysis of variance (ANOVA) and is a test of hypothesis that is appropriate to compare means of a continuous variable in two or more independent comparison groups. For example, in some clinical trials there are more than two comparison groups. In a clinical trial to evaluate a new medication for asthma, investigators might compare an experimental medication to a placebo and to a standard treatment (i.e., a medication currently being used). In an observational study such as the Framingham Heart Study, it might be of interest to compare mean blood pressure or mean cholesterol levels in persons who are underweight, normal weight, overweight and obese.  

The technique to test for a difference in more than two independent means is an extension of the two independent samples procedure discussed previously which applies when there are exactly two independent comparison groups. The ANOVA technique applies when there are two or more than two independent groups. The ANOVA procedure is used to compare the means of the comparison groups and is conducted using the same five step approach used in the scenarios discussed in previous sections. Because there are more than two groups, however, the computation of the test statistic is more involved. The test statistic must take into account the sample sizes, sample means and sample standard deviations in each of the comparison groups.

If one is examining the means observed among, say three groups, it might be tempting to perform three separate group to group comparisons, but this approach is incorrect because each of these comparisons fails to take into account the total data, and it increases the likelihood of incorrectly concluding that there are statistically significate differences, since each comparison adds to the probability of a type I error. Analysis of variance avoids these problemss by asking a more global question, i.e., whether there are significant differences among the groups, without addressing differences between any two groups in particular (although there are additional tests that can do this if the analysis of variance indicates that there are differences among the groups).

The fundamental strategy of ANOVA is to systematically examine variability within groups being compared and also examine variability among the groups being compared.

Learning Objectives

After completing this module, the student will be able to:

• Perform analysis of variance by hand
• Appropriately interpret results of analysis of variance tests
• Distinguish between one and two factor analysis of variance tests
• Identify the appropriate hypothesis testing procedure based on type of outcome variable and number of samples

The ANOVA Approach

Consider an example with four independent groups and a continuous outcome measure. The independent groups might be defined by a particular characteristic of the participants such as BMI (e.g., underweight, normal weight, overweight, obese) or by the investigator (e.g., randomizing participants to one of four competing treatments, call them A, B, C and D). Suppose that the outcome is systolic blood pressure, and we wish to test whether there is a statistically significant difference in mean systolic blood pressures among the four groups. The sample data are organized as follows:

The hypotheses of interest in an ANOVA are as follows:

• H 0 : μ 1 = μ 2 = μ 3 ... = μ k
• H 1 : Means are not all equal.

where k = the number of independent comparison groups.

In this example, the hypotheses are:

• H 0 : μ 1 = μ 2 = μ 3 = μ 4
• H 1 : The means are not all equal.

The null hypothesis in ANOVA is always that there is no difference in means. The research or alternative hypothesis is always that the means are not all equal and is usually written in words rather than in mathematical symbols. The research hypothesis captures any difference in means and includes, for example, the situation where all four means are unequal, where one is different from the other three, where two are different, and so on. The alternative hypothesis, as shown above, capture all possible situations other than equality of all means specified in the null hypothesis.

Test Statistic for ANOVA

The test statistic for testing H 0 : μ 1 = μ 2 = ... =   μ k is:

and the critical value is found in a table of probability values for the F distribution with (degrees of freedom) df 1 = k-1, df 2 =N-k. The table can be found in "Other Resources" on the left side of the pages.

NOTE: The test statistic F assumes equal variability in the k populations (i.e., the population variances are equal, or s 1 2 = s 2 2 = ... = s k 2 ). This means that the outcome is equally variable in each of the comparison populations. This assumption is the same as that assumed for appropriate use of the test statistic to test equality of two independent means. It is possible to assess the likelihood that the assumption of equal variances is true and the test can be conducted in most statistical computing packages. If the variability in the k comparison groups is not similar, then alternative techniques must be used.

The F statistic is computed by taking the ratio of what is called the "between treatment" variability to the "residual or error" variability. This is where the name of the procedure originates. In analysis of variance we are testing for a difference in means (H 0 : means are all equal versus H 1 : means are not all equal) by evaluating variability in the data. The numerator captures between treatment variability (i.e., differences among the sample means) and the denominator contains an estimate of the variability in the outcome. The test statistic is a measure that allows us to assess whether the differences among the sample means (numerator) are more than would be expected by chance if the null hypothesis is true. Recall in the two independent sample test, the test statistic was computed by taking the ratio of the difference in sample means (numerator) to the variability in the outcome (estimated by Sp).  

The decision rule for the F test in ANOVA is set up in a similar way to decision rules we established for t tests. The decision rule again depends on the level of significance and the degrees of freedom. The F statistic has two degrees of freedom. These are denoted df 1 and df 2 , and called the numerator and denominator degrees of freedom, respectively. The degrees of freedom are defined as follows:

df 1 = k-1 and df 2 =N-k,

where k is the number of comparison groups and N is the total number of observations in the analysis.   If the null hypothesis is true, the between treatment variation (numerator) will not exceed the residual or error variation (denominator) and the F statistic will small. If the null hypothesis is false, then the F statistic will be large. The rejection region for the F test is always in the upper (right-hand) tail of the distribution as shown below.

Rejection Region for F   Test with a =0.05, df 1 =3 and df 2 =36 (k=4, N=40)

For the scenario depicted here, the decision rule is: Reject H 0 if F > 2.87.

The ANOVA Procedure

We will next illustrate the ANOVA procedure using the five step approach. Because the computation of the test statistic is involved, the computations are often organized in an ANOVA table. The ANOVA table breaks down the components of variation in the data into variation between treatments and error or residual variation. Statistical computing packages also produce ANOVA tables as part of their standard output for ANOVA, and the ANOVA table is set up as follows: 

where  

• X = individual observation,
• k = the number of treatments or independent comparison groups, and
• N = total number of observations or total sample size.

The ANOVA table above is organized as follows.

• The first column is entitled "Source of Variation" and delineates the between treatment and error or residual variation. The total variation is the sum of the between treatment and error variation.
• The second column is entitled "Sums of Squares (SS)" . The between treatment sums of squares is

and is computed by summing the squared differences between each treatment (or group) mean and the overall mean. The squared differences are weighted by the sample sizes per group (n j ). The error sums of squares is:

and is computed by summing the squared differences between each observation and its group mean (i.e., the squared differences between each observation in group 1 and the group 1 mean, the squared differences between each observation in group 2 and the group 2 mean, and so on). The double summation ( SS ) indicates summation of the squared differences within each treatment and then summation of these totals across treatments to produce a single value. (This will be illustrated in the following examples). The total sums of squares is:

and is computed by summing the squared differences between each observation and the overall sample mean. In an ANOVA, data are organized by comparison or treatment groups. If all of the data were pooled into a single sample, SST would reflect the numerator of the sample variance computed on the pooled or total sample. SST does not figure into the F statistic directly. However, SST = SSB + SSE, thus if two sums of squares are known, the third can be computed from the other two.

• The third column contains degrees of freedom . The between treatment degrees of freedom is df 1 = k-1. The error degrees of freedom is df 2 = N - k. The total degrees of freedom is N-1 (and it is also true that (k-1) + (N-k) = N-1).
• The fourth column contains "Mean Squares (MS)" which are computed by dividing sums of squares (SS) by degrees of freedom (df), row by row. Specifically, MSB=SSB/(k-1) and MSE=SSE/(N-k). Dividing SST/(N-1) produces the variance of the total sample. The F statistic is in the rightmost column of the ANOVA table and is computed by taking the ratio of MSB/MSE.  

A clinical trial is run to compare weight loss programs and participants are randomly assigned to one of the comparison programs and are counseled on the details of the assigned program. Participants follow the assigned program for 8 weeks. The outcome of interest is weight loss, defined as the difference in weight measured at the start of the study (baseline) and weight measured at the end of the study (8 weeks), measured in pounds.  

Three popular weight loss programs are considered. The first is a low calorie diet. The second is a low fat diet and the third is a low carbohydrate diet. For comparison purposes, a fourth group is considered as a control group. Participants in the fourth group are told that they are participating in a study of healthy behaviors with weight loss only one component of interest. The control group is included here to assess the placebo effect (i.e., weight loss due to simply participating in the study). A total of twenty patients agree to participate in the study and are randomly assigned to one of the four diet groups. Weights are measured at baseline and patients are counseled on the proper implementation of the assigned diet (with the exception of the control group). After 8 weeks, each patient's weight is again measured and the difference in weights is computed by subtracting the 8 week weight from the baseline weight. Positive differences indicate weight losses and negative differences indicate weight gains. For interpretation purposes, we refer to the differences in weights as weight losses and the observed weight losses are shown below.

Is there a statistically significant difference in the mean weight loss among the four diets?  We will run the ANOVA using the five-step approach.

• Step 1. Set up hypotheses and determine level of significance

H 0 : μ 1 = μ 2 = μ 3 = μ 4 H 1 : Means are not all equal              α=0.05

• Step 2. Select the appropriate test statistic.  

The test statistic is the F statistic for ANOVA, F=MSB/MSE.

• Step 3. Set up decision rule.  

The appropriate critical value can be found in a table of probabilities for the F distribution(see "Other Resources"). In order to determine the critical value of F we need degrees of freedom, df 1 =k-1 and df 2 =N-k. In this example, df 1 =k-1=4-1=3 and df 2 =N-k=20-4=16. The critical value is 3.24 and the decision rule is as follows: Reject H 0 if F > 3.24.

• Step 4. Compute the test statistic.  

To organize our computations we complete the ANOVA table. In order to compute the sums of squares we must first compute the sample means for each group and the overall mean based on the total sample.  

We can now compute

So, in this case:

Next we compute,

SSE requires computing the squared differences between each observation and its group mean. We will compute SSE in parts. For the participants in the low calorie diet:  

For the participants in the low fat diet:  

For the participants in the low carbohydrate diet:  

For the participants in the control group:

We can now construct the ANOVA table .

• Step 5. Conclusion.  

We reject H 0 because 8.43 > 3.24. We have statistically significant evidence at α=0.05 to show that there is a difference in mean weight loss among the four diets.    

ANOVA is a test that provides a global assessment of a statistical difference in more than two independent means. In this example, we find that there is a statistically significant difference in mean weight loss among the four diets considered. In addition to reporting the results of the statistical test of hypothesis (i.e., that there is a statistically significant difference in mean weight losses at α=0.05), investigators should also report the observed sample means to facilitate interpretation of the results. In this example, participants in the low calorie diet lost an average of 6.6 pounds over 8 weeks, as compared to 3.0 and 3.4 pounds in the low fat and low carbohydrate groups, respectively. Participants in the control group lost an average of 1.2 pounds which could be called the placebo effect because these participants were not participating in an active arm of the trial specifically targeted for weight loss. Are the observed weight losses clinically meaningful?

Another ANOVA Example

Calcium is an essential mineral that regulates the heart, is important for blood clotting and for building healthy bones. The National Osteoporosis Foundation recommends a daily calcium intake of 1000-1200 mg/day for adult men and women. While calcium is contained in some foods, most adults do not get enough calcium in their diets and take supplements. Unfortunately some of the supplements have side effects such as gastric distress, making them difficult for some patients to take on a regular basis.  

 A study is designed to test whether there is a difference in mean daily calcium intake in adults with normal bone density, adults with osteopenia (a low bone density which may lead to osteoporosis) and adults with osteoporosis. Adults 60 years of age with normal bone density, osteopenia and osteoporosis are selected at random from hospital records and invited to participate in the study. Each participant's daily calcium intake is measured based on reported food intake and supplements. The data are shown below.   

Is there a statistically significant difference in mean calcium intake in patients with normal bone density as compared to patients with osteopenia and osteoporosis? We will run the ANOVA using the five-step approach.

H 0 : μ 1 = μ 2 = μ 3 H 1 : Means are not all equal                            α=0.05

In order to determine the critical value of F we need degrees of freedom, df 1 =k-1 and df 2 =N-k.   In this example, df 1 =k-1=3-1=2 and df 2 =N-k=18-3=15. The critical value is 3.68 and the decision rule is as follows: Reject H 0 if F > 3.68.

To organize our computations we will complete the ANOVA table. In order to compute the sums of squares we must first compute the sample means for each group and the overall mean.  

 If we pool all N=18 observations, the overall mean is 817.8.

We can now compute:

Substituting:

SSE requires computing the squared differences between each observation and its group mean. We will compute SSE in parts. For the participants with normal bone density:

For participants with osteopenia:

For participants with osteoporosis:

We do not reject H 0 because 1.395 < 3.68. We do not have statistically significant evidence at a =0.05 to show that there is a difference in mean calcium intake in patients with normal bone density as compared to osteopenia and osterporosis. Are the differences in mean calcium intake clinically meaningful? If so, what might account for the lack of statistical significance?

One-Way ANOVA in R

The video below by Mike Marin demonstrates how to perform analysis of variance in R. It also covers some other statistical issues, but the initial part of the video will be useful to you.

Two-Factor ANOVA

The ANOVA tests described above are called one-factor ANOVAs. There is one treatment or grouping factor with k > 2 levels and we wish to compare the means across the different categories of this factor. The factor might represent different diets, different classifications of risk for disease (e.g., osteoporosis), different medical treatments, different age groups, or different racial/ethnic groups. There are situations where it may be of interest to compare means of a continuous outcome across two or more factors. For example, suppose a clinical trial is designed to compare five different treatments for joint pain in patients with osteoarthritis. Investigators might also hypothesize that there are differences in the outcome by sex. This is an example of a two-factor ANOVA where the factors are treatment (with 5 levels) and sex (with 2 levels). In the two-factor ANOVA, investigators can assess whether there are differences in means due to the treatment, by sex or whether there is a difference in outcomes by the combination or interaction of treatment and sex. Higher order ANOVAs are conducted in the same way as one-factor ANOVAs presented here and the computations are again organized in ANOVA tables with more rows to distinguish the different sources of variation (e.g., between treatments, between men and women). The following example illustrates the approach.

Consider the clinical trial outlined above in which three competing treatments for joint pain are compared in terms of their mean time to pain relief in patients with osteoarthritis. Because investigators hypothesize that there may be a difference in time to pain relief in men versus women, they randomly assign 15 participating men to one of the three competing treatments and randomly assign 15 participating women to one of the three competing treatments (i.e., stratified randomization). Participating men and women do not know to which treatment they are assigned. They are instructed to take the assigned medication when they experience joint pain and to record the time, in minutes, until the pain subsides. The data (times to pain relief) are shown below and are organized by the assigned treatment and sex of the participant.

Table of Time to Pain Relief by Treatment and Sex

The analysis in two-factor ANOVA is similar to that illustrated above for one-factor ANOVA. The computations are again organized in an ANOVA table, but the total variation is partitioned into that due to the main effect of treatment, the main effect of sex and the interaction effect. The results of the analysis are shown below (and were generated with a statistical computing package - here we focus on interpretation). 

 ANOVA Table for Two-Factor ANOVA

There are 4 statistical tests in the ANOVA table above. The first test is an overall test to assess whether there is a difference among the 6 cell means (cells are defined by treatment and sex). The F statistic is 20.7 and is highly statistically significant with p=0.0001. When the overall test is significant, focus then turns to the factors that may be driving the significance (in this example, treatment, sex or the interaction between the two). The next three statistical tests assess the significance of the main effect of treatment, the main effect of sex and the interaction effect. In this example, there is a highly significant main effect of treatment (p=0.0001) and a highly significant main effect of sex (p=0.0001). The interaction between the two does not reach statistical significance (p=0.91). The table below contains the mean times to pain relief in each of the treatments for men and women (Note that each sample mean is computed on the 5 observations measured under that experimental condition).  

Mean Time to Pain Relief by Treatment and Gender

Treatment A appears to be the most efficacious treatment for both men and women. The mean times to relief are lower in Treatment A for both men and women and highest in Treatment C for both men and women. Across all treatments, women report longer times to pain relief (See below).  

Notice that there is the same pattern of time to pain relief across treatments in both men and women (treatment effect). There is also a sex effect - specifically, time to pain relief is longer in women in every treatment.  

Suppose that the same clinical trial is replicated in a second clinical site and the following data are observed.

Table - Time to Pain Relief by Treatment and Sex - Clinical Site 2

The ANOVA table for the data measured in clinical site 2 is shown below.

Table - Summary of Two-Factor ANOVA - Clinical Site 2

Notice that the overall test is significant (F=19.4, p=0.0001), there is a significant treatment effect, sex effect and a highly significant interaction effect. The table below contains the mean times to relief in each of the treatments for men and women.  

Table - Mean Time to Pain Relief by Treatment and Gender - Clinical Site 2

Notice that now the differences in mean time to pain relief among the treatments depend on sex. Among men, the mean time to pain relief is highest in Treatment A and lowest in Treatment C. Among women, the reverse is true. This is an interaction effect (see below).  

Notice above that the treatment effect varies depending on sex. Thus, we cannot summarize an overall treatment effect (in men, treatment C is best, in women, treatment A is best).    

When interaction effects are present, some investigators do not examine main effects (i.e., do not test for treatment effect because the effect of treatment depends on sex). This issue is complex and is discussed in more detail in a later module. 

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Understanding Hypothesis Testing

Hypothesis testing involves formulating assumptions about population parameters based on sample statistics and rigorously evaluating these assumptions against empirical evidence. This article sheds light on the significance of hypothesis testing and the critical steps involved in the process.

What is Hypothesis Testing?

A hypothesis is an assumption or idea, specifically a statistical claim about an unknown population parameter. For example, a judge assumes a person is innocent and verifies this by reviewing evidence and hearing testimony before reaching a verdict.

Hypothesis testing is a statistical method that is used to make a statistical decision using experimental data. Hypothesis testing is basically an assumption that we make about a population parameter. It evaluates two mutually exclusive statements about a population to determine which statement is best supported by the sample data. 

To test the validity of the claim or assumption about the population parameter:

• A sample is drawn from the population and analyzed.
• The results of the analysis are used to decide whether the claim is true or not.

Example: You say an average height in the class is 30 or a boy is taller than a girl. All of these is an assumption that we are assuming, and we need some statistical way to prove these. We need some mathematical conclusion whatever we are assuming is true.

Defining Hypotheses

• Null hypothesis (H 0 ): In statistics, the null hypothesis is a general statement or default position that there is no relationship between two measured cases or no relationship among groups. In other words, it is a basic assumption or made based on the problem knowledge. Example : A company’s mean production is 50 units/per da H 0 : [Tex]\mu [/Tex] = 50.
• Alternative hypothesis (H 1 ): The alternative hypothesis is the hypothesis used in hypothesis testing that is contrary to the null hypothesis.  Example: A company’s production is not equal to 50 units/per day i.e. H 1 : [Tex]\mu [/Tex] [Tex]\ne [/Tex] 50.

Key Terms of Hypothesis Testing

• Level of significance : It refers to the degree of significance in which we accept or reject the null hypothesis. 100% accuracy is not possible for accepting a hypothesis, so we, therefore, select a level of significance that is usually 5%. This is normally denoted with  [Tex]\alpha[/Tex] and generally, it is 0.05 or 5%, which means your output should be 95% confident to give a similar kind of result in each sample.
• P-value: The P value , or calculated probability, is the probability of finding the observed/extreme results when the null hypothesis(H0) of a study-given problem is true. If your P-value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample claims to support the alternative hypothesis.
• Test Statistic: The test statistic is a numerical value calculated from sample data during a hypothesis test, used to determine whether to reject the null hypothesis. It is compared to a critical value or p-value to make decisions about the statistical significance of the observed results.
• Critical value : The critical value in statistics is a threshold or cutoff point used to determine whether to reject the null hypothesis in a hypothesis test.
• Degrees of freedom: Degrees of freedom are associated with the variability or freedom one has in estimating a parameter. The degrees of freedom are related to the sample size and determine the shape.

Why do we use Hypothesis Testing?

Hypothesis testing is an important procedure in statistics. Hypothesis testing evaluates two mutually exclusive population statements to determine which statement is most supported by sample data. When we say that the findings are statistically significant, thanks to hypothesis testing. 

One-Tailed and Two-Tailed Test

One tailed test focuses on one direction, either greater than or less than a specified value. We use a one-tailed test when there is a clear directional expectation based on prior knowledge or theory. The critical region is located on only one side of the distribution curve. If the sample falls into this critical region, the null hypothesis is rejected in favor of the alternative hypothesis.

One-Tailed Test

There are two types of one-tailed test:

• Left-Tailed (Left-Sided) Test: The alternative hypothesis asserts that the true parameter value is less than the null hypothesis. Example: H 0 ​: [Tex]\mu \geq 50 [/Tex] and H 1 : [Tex]\mu < 50 [/Tex]
• Right-Tailed (Right-Sided) Test : The alternative hypothesis asserts that the true parameter value is greater than the null hypothesis. Example: H 0 : [Tex]\mu \leq50 [/Tex] and H 1 : [Tex]\mu > 50 [/Tex]

Two-Tailed Test

A two-tailed test considers both directions, greater than and less than a specified value.We use a two-tailed test when there is no specific directional expectation, and want to detect any significant difference.

Example: H 0 : [Tex]\mu = [/Tex] 50 and H 1 : [Tex]\mu \neq 50 [/Tex]

To delve deeper into differences into both types of test: Refer to link

What are Type 1 and Type 2 errors in Hypothesis Testing?

In hypothesis testing, Type I and Type II errors are two possible errors that researchers can make when drawing conclusions about a population based on a sample of data. These errors are associated with the decisions made regarding the null hypothesis and the alternative hypothesis.

• Type I error: When we reject the null hypothesis, although that hypothesis was true. Type I error is denoted by alpha( [Tex]\alpha [/Tex] ).
• Type II errors : When we accept the null hypothesis, but it is false. Type II errors are denoted by beta( [Tex]\beta [/Tex] ).

How does Hypothesis Testing work?

Step 1: define null and alternative hypothesis.

State the null hypothesis ( [Tex]H_0 [/Tex] ), representing no effect, and the alternative hypothesis ( [Tex]H_1 [/Tex] ​), suggesting an effect or difference.

We first identify the problem about which we want to make an assumption keeping in mind that our assumption should be contradictory to one another, assuming Normally distributed data.

Step 2 – Choose significance level

Select a significance level ( [Tex]\alpha [/Tex] ), typically 0.05, to determine the threshold for rejecting the null hypothesis. It provides validity to our hypothesis test, ensuring that we have sufficient data to back up our claims. Usually, we determine our significance level beforehand of the test. The p-value is the criterion used to calculate our significance value.

Step 3 – Collect and Analyze data.

Gather relevant data through observation or experimentation. Analyze the data using appropriate statistical methods to obtain a test statistic.

Step 4-Calculate Test Statistic

The data for the tests are evaluated in this step we look for various scores based on the characteristics of data. The choice of the test statistic depends on the type of hypothesis test being conducted.

There are various hypothesis tests, each appropriate for various goal to calculate our test. This could be a Z-test , Chi-square , T-test , and so on.

• Z-test : If population means and standard deviations are known. Z-statistic is commonly used.
• t-test : If population standard deviations are unknown. and sample size is small than t-test statistic is more appropriate.
• Chi-square test : Chi-square test is used for categorical data or for testing independence in contingency tables
• F-test : F-test is often used in analysis of variance (ANOVA) to compare variances or test the equality of means across multiple groups.

We have a smaller dataset, So, T-test is more appropriate to test our hypothesis.

T-statistic is a measure of the difference between the means of two groups relative to the variability within each group. It is calculated as the difference between the sample means divided by the standard error of the difference. It is also known as the t-value or t-score.

Step 5 – Comparing Test Statistic:

In this stage, we decide where we should accept the null hypothesis or reject the null hypothesis. There are two ways to decide where we should accept or reject the null hypothesis.

Method A: Using Crtical values

Comparing the test statistic and tabulated critical value we have,

• If Test Statistic>Critical Value: Reject the null hypothesis.
• If Test Statistic≤Critical Value: Fail to reject the null hypothesis.

Note: Critical values are predetermined threshold values that are used to make a decision in hypothesis testing. To determine critical values for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Method B: Using P-values

We can also come to an conclusion using the p-value,

• If the p-value is less than or equal to the significance level i.e. ( [Tex]p\leq\alpha [/Tex] ), you reject the null hypothesis. This indicates that the observed results are unlikely to have occurred by chance alone, providing evidence in favor of the alternative hypothesis.
• If the p-value is greater than the significance level i.e. ( [Tex]p\geq \alpha[/Tex] ), you fail to reject the null hypothesis. This suggests that the observed results are consistent with what would be expected under the null hypothesis.

Note : The p-value is the probability of obtaining a test statistic as extreme as, or more extreme than, the one observed in the sample, assuming the null hypothesis is true. To determine p-value for hypothesis testing, we typically refer to a statistical distribution table , such as the normal distribution or t-distribution tables based on.

Step 7- Interpret the Results

At last, we can conclude our experiment using method A or B.

Calculating test statistic

To validate our hypothesis about a population parameter we use statistical functions . We use the z-score, p-value, and level of significance(alpha) to make evidence for our hypothesis for normally distributed data .

1. Z-statistics:

When population means and standard deviations are known.

[Tex]z = \frac{\bar{x} – \mu}{\frac{\sigma}{\sqrt{n}}}[/Tex]

• [Tex]\bar{x} [/Tex] is the sample mean,
• μ represents the population mean, 
• σ is the standard deviation
• and n is the size of the sample.

2. T-Statistics

T test is used when n<30,

t-statistic calculation is given by:

[Tex]t=\frac{x̄-μ}{s/\sqrt{n}} [/Tex]

• t = t-score,
• x̄ = sample mean
• μ = population mean,
• s = standard deviation of the sample,
• n = sample size

3. Chi-Square Test

Chi-Square Test for Independence categorical Data (Non-normally distributed) using:

[Tex]\chi^2 = \sum \frac{(O_{ij} – E_{ij})^2}{E_{ij}}[/Tex]

• [Tex]O_{ij}[/Tex] is the observed frequency in cell [Tex]{ij} [/Tex]
• i,j are the rows and columns index respectively.
• [Tex]E_{ij}[/Tex] is the expected frequency in cell [Tex]{ij}[/Tex] , calculated as : [Tex]\frac{{\text{{Row total}} \times \text{{Column total}}}}{{\text{{Total observations}}}}[/Tex]

Real life Examples of Hypothesis Testing

Let’s examine hypothesis testing using two real life situations,

Case A: D oes a New Drug Affect Blood Pressure?

Imagine a pharmaceutical company has developed a new drug that they believe can effectively lower blood pressure in patients with hypertension. Before bringing the drug to market, they need to conduct a study to assess its impact on blood pressure.

• Before Treatment: 120, 122, 118, 130, 125, 128, 115, 121, 123, 119
• After Treatment: 115, 120, 112, 128, 122, 125, 110, 117, 119, 114

Step 1 : Define the Hypothesis

• Null Hypothesis : (H 0 )The new drug has no effect on blood pressure.
• Alternate Hypothesis : (H 1 )The new drug has an effect on blood pressure.

Step 2: Define the Significance level

Let’s consider the Significance level at 0.05, indicating rejection of the null hypothesis.

If the evidence suggests less than a 5% chance of observing the results due to random variation.

Step 3 : Compute the test statistic

Using paired T-test analyze the data to obtain a test statistic and a p-value.

The test statistic (e.g., T-statistic) is calculated based on the differences between blood pressure measurements before and after treatment.

t = m/(s/√n)

• m  = mean of the difference i.e X after, X before
• s  = standard deviation of the difference (d) i.e d i ​= X after, i ​− X before,
• n  = sample size,

then, m= -3.9, s= 1.8 and n= 10

we, calculate the , T-statistic = -9 based on the formula for paired t test

Step 4: Find the p-value

The calculated t-statistic is -9 and degrees of freedom df = 9, you can find the p-value using statistical software or a t-distribution table.

thus, p-value = 8.538051223166285e-06

Step 5: Result

• If the p-value is less than or equal to 0.05, the researchers reject the null hypothesis.
• If the p-value is greater than 0.05, they fail to reject the null hypothesis.

Conclusion: Since the p-value (8.538051223166285e-06) is less than the significance level (0.05), the researchers reject the null hypothesis. There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

Python Implementation of Case A

Let’s create hypothesis testing with python, where we are testing whether a new drug affects blood pressure. For this example, we will use a paired T-test. We’ll use the scipy.stats library for the T-test.

Scipy is a mathematical library in Python that is mostly used for mathematical equations and computations.

We will implement our first real life problem via python,

import numpy as np from scipy import stats # Data before_treatment = np . array ([ 120 , 122 , 118 , 130 , 125 , 128 , 115 , 121 , 123 , 119 ]) after_treatment = np . array ([ 115 , 120 , 112 , 128 , 122 , 125 , 110 , 117 , 119 , 114 ]) # Step 1: Null and Alternate Hypotheses # Null Hypothesis: The new drug has no effect on blood pressure. # Alternate Hypothesis: The new drug has an effect on blood pressure. null_hypothesis = "The new drug has no effect on blood pressure." alternate_hypothesis = "The new drug has an effect on blood pressure." # Step 2: Significance Level alpha = 0.05 # Step 3: Paired T-test t_statistic , p_value = stats . ttest_rel ( after_treatment , before_treatment ) # Step 4: Calculate T-statistic manually m = np . mean ( after_treatment - before_treatment ) s = np . std ( after_treatment - before_treatment , ddof = 1 ) # using ddof=1 for sample standard deviation n = len ( before_treatment ) t_statistic_manual = m / ( s / np . sqrt ( n )) # Step 5: Decision if p_value <= alpha : decision = "Reject" else : decision = "Fail to reject" # Conclusion if decision == "Reject" : conclusion = "There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different." else : conclusion = "There is insufficient evidence to claim a significant difference in average blood pressure before and after treatment with the new drug." # Display results print ( "T-statistic (from scipy):" , t_statistic ) print ( "P-value (from scipy):" , p_value ) print ( "T-statistic (calculated manually):" , t_statistic_manual ) print ( f "Decision: { decision } the null hypothesis at alpha= { alpha } ." ) print ( "Conclusion:" , conclusion )

T-statistic (from scipy): -9.0 P-value (from scipy): 8.538051223166285e-06 T-statistic (calculated manually): -9.0 Decision: Reject the null hypothesis at alpha=0.05. Conclusion: There is statistically significant evidence that the average blood pressure before and after treatment with the new drug is different.

In the above example, given the T-statistic of approximately -9 and an extremely small p-value, the results indicate a strong case to reject the null hypothesis at a significance level of 0.05. 

• The results suggest that the new drug, treatment, or intervention has a significant effect on lowering blood pressure.
• The negative T-statistic indicates that the mean blood pressure after treatment is significantly lower than the assumed population mean before treatment.

Case B : Cholesterol level in a population

Data: A sample of 25 individuals is taken, and their cholesterol levels are measured.

Cholesterol Levels (mg/dL): 205, 198, 210, 190, 215, 205, 200, 192, 198, 205, 198, 202, 208, 200, 205, 198, 205, 210, 192, 205, 198, 205, 210, 192, 205.

Populations Mean = 200

Population Standard Deviation (σ): 5 mg/dL(given for this problem)

Step 1: Define the Hypothesis

• Null Hypothesis (H 0 ): The average cholesterol level in a population is 200 mg/dL.
• Alternate Hypothesis (H 1 ): The average cholesterol level in a population is different from 200 mg/dL.

As the direction of deviation is not given , we assume a two-tailed test, and based on a normal distribution table, the critical values for a significance level of 0.05 (two-tailed) can be calculated through the z-table and are approximately -1.96 and 1.96.

The test statistic is calculated by using the z formula Z = [Tex](203.8 – 200) / (5 \div \sqrt{25}) [/Tex] ​ and we get accordingly , Z =2.039999999999992.

Step 4: Result

Since the absolute value of the test statistic (2.04) is greater than the critical value (1.96), we reject the null hypothesis. And conclude that, there is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL

Python Implementation of Case B

import scipy.stats as stats import math import numpy as np # Given data sample_data = np . array ( [ 205 , 198 , 210 , 190 , 215 , 205 , 200 , 192 , 198 , 205 , 198 , 202 , 208 , 200 , 205 , 198 , 205 , 210 , 192 , 205 , 198 , 205 , 210 , 192 , 205 ]) population_std_dev = 5 population_mean = 200 sample_size = len ( sample_data ) # Step 1: Define the Hypotheses # Null Hypothesis (H0): The average cholesterol level in a population is 200 mg/dL. # Alternate Hypothesis (H1): The average cholesterol level in a population is different from 200 mg/dL. # Step 2: Define the Significance Level alpha = 0.05 # Two-tailed test # Critical values for a significance level of 0.05 (two-tailed) critical_value_left = stats . norm . ppf ( alpha / 2 ) critical_value_right = - critical_value_left # Step 3: Compute the test statistic sample_mean = sample_data . mean () z_score = ( sample_mean - population_mean ) / \ ( population_std_dev / math . sqrt ( sample_size )) # Step 4: Result # Check if the absolute value of the test statistic is greater than the critical values if abs ( z_score ) > max ( abs ( critical_value_left ), abs ( critical_value_right )): print ( "Reject the null hypothesis." ) print ( "There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL." ) else : print ( "Fail to reject the null hypothesis." ) print ( "There is not enough evidence to conclude that the average cholesterol level in the population is different from 200 mg/dL." )

Reject the null hypothesis. There is statistically significant evidence that the average cholesterol level in the population is different from 200 mg/dL.

Limitations of Hypothesis Testing

• Although a useful technique, hypothesis testing does not offer a comprehensive grasp of the topic being studied. Without fully reflecting the intricacy or whole context of the phenomena, it concentrates on certain hypotheses and statistical significance.
• The accuracy of hypothesis testing results is contingent on the quality of available data and the appropriateness of statistical methods used. Inaccurate data or poorly formulated hypotheses can lead to incorrect conclusions.
• Relying solely on hypothesis testing may cause analysts to overlook significant patterns or relationships in the data that are not captured by the specific hypotheses being tested. This limitation underscores the importance of complimenting hypothesis testing with other analytical approaches.

Hypothesis testing stands as a cornerstone in statistical analysis, enabling data scientists to navigate uncertainties and draw credible inferences from sample data. By systematically defining null and alternative hypotheses, choosing significance levels, and leveraging statistical tests, researchers can assess the validity of their assumptions. The article also elucidates the critical distinction between Type I and Type II errors, providing a comprehensive understanding of the nuanced decision-making process inherent in hypothesis testing. The real-life example of testing a new drug’s effect on blood pressure using a paired T-test showcases the practical application of these principles, underscoring the importance of statistical rigor in data-driven decision-making.

Frequently Asked Questions (FAQs)

1. what are the 3 types of hypothesis test.

There are three types of hypothesis tests: right-tailed, left-tailed, and two-tailed. Right-tailed tests assess if a parameter is greater, left-tailed if lesser. Two-tailed tests check for non-directional differences, greater or lesser.

2.What are the 4 components of hypothesis testing?

Null Hypothesis ( [Tex]H_o [/Tex] ): No effect or difference exists. Alternative Hypothesis ( [Tex]H_1 [/Tex] ): An effect or difference exists. Significance Level ( [Tex]\alpha [/Tex] ): Risk of rejecting null hypothesis when it’s true (Type I error). Test Statistic: Numerical value representing observed evidence against null hypothesis.

3.What is hypothesis testing in ML?

Statistical method to evaluate the performance and validity of machine learning models. Tests specific hypotheses about model behavior, like whether features influence predictions or if a model generalizes well to unseen data.

4.What is the difference between Pytest and hypothesis in Python?

Pytest purposes general testing framework for Python code while Hypothesis is a Property-based testing framework for Python, focusing on generating test cases based on specified properties of the code.

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Computer Science > Cryptography and Security

Title: a statistical viewpoint on differential privacy: hypothesis testing, representation and blackwell's theorem.

Abstract: Differential privacy is widely considered the formal privacy for privacy-preserving data analysis due to its robust and rigorous guarantees, with increasingly broad adoption in public services, academia, and industry. Despite originating in the cryptographic context, in this review paper we argue that, fundamentally, differential privacy can be considered a \textit{pure} statistical concept. By leveraging a theorem due to David Blackwell, our focus is to demonstrate that the definition of differential privacy can be formally motivated from a hypothesis testing perspective, thereby showing that hypothesis testing is not merely convenient but also the right language for reasoning about differential privacy. This insight leads to the definition of $f$-differential privacy, which extends other differential privacy definitions through a representation theorem. We review techniques that render $f$-differential privacy a unified framework for analyzing privacy bounds in data analysis and machine learning. Applications of this differential privacy definition to private deep learning, private convex optimization, shuffled mechanisms, and U.S.~Census data are discussed to highlight the benefits of analyzing privacy bounds under this framework compared to existing alternatives.

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